ORF » Species Summary » Homo sapiens » CD28 cDNA ORF clone

CD28 cDNA ORF clone, Homo sapiens(human)

The following CD28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CD28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu22476 NM_006139.4
Latest version!		 Homo sapiens CD28 molecule (CD28), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$49.50-$69.30
$99.00
OHu07814 NM_001243078.1
Latest version!		 Homo sapiens CD28 molecule (CD28), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $69.30
$99.00
OHu07986 NM_001243077.2
Latest version!		 Homo sapiens CD28 molecule (CD28), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $69.30
$99.00
OHu56712 XM_011512194.2
Latest version!		 Homo sapiens CD28 molecule (CD28), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OHu56713 XM_011512195.3
Latest version!		 Homo sapiens CD28 molecule (CD28), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $69.30
$99.00
OHu56715 XM_011512197.2
Latest version!		 Homo sapiens CD28 molecule (CD28), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $69.30
$99.00
View Old Accession Versions >>
OHu22476 NM_006139.3 Homo sapiens CD28 molecule (CD28), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$49.50-$69.30
$99.00
OHu56713 XM_011512195.2 Homo sapiens CD28 molecule (CD28), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $69.30
$99.00
OHu07986 NM_001243077.1 Homo sapiens CD28 molecule (CD28), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $69.30
$99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CD28 ( NM_006139.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006130.1 Homo sapiens CD28 molecule (CD28), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu22476
    Clone ID Related Accession (Same CDS sequence) NM_006139.4 , NM_006139.3
    Accession Version NM_006139.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 663bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1577635200000
    Organism Homo sapiens(human)
    Product T-cell-specific surface glycoprotein CD28 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC125238.5, AK292986.1, J02988.1, DA939711.1, AW241446.1, AA311148.1 and BF509568.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_006139.3. Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J02988.1, ERR279829.6684.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000324106.9/ ENSP00000324890.7 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCTCAGGC TGCTCTTGGC TCTCAACTTA TTCCCTTCAA TTCAAGTAAC AGGAAACAAG 
    ATTTTGGTGA AGCAGTCGCC CATGCTTGTA GCGTACGACA ATGCGGTCAA CCTTAGCTGC 
    AAGTATTCCT ACAATCTCTT CTCAAGGGAG TTCCGGGCAT CCCTTCACAA AGGACTGGAT 
    AGTGCTGTGG AAGTCTGTGT TGTATATGGG AATTACTCCC AGCAGCTTCA GGTTTACTCA 
    AAAACGGGGT TCAACTGTGA TGGGAAATTG GGCAATGAAT CAGTGACATT CTACCTCCAG 
    AATTTGTATG TTAACCAAAC AGATATTTAC TTCTGCAAAA TTGAAGTTAT GTATCCTCCT 
    CCTTACCTAG ACAATGAGAA GAGCAATGGA ACCATTATCC ATGTGAAAGG GAAACACCTT 
    TGTCCAAGTC CCCTATTTCC CGGACCTTCT AAGCCCTTTT GGGTGCTGGT GGTGGTTGGT 
    GGAGTCCTGG CTTGCTATAG CTTGCTAGTA ACAGTGGCCT TTATTATTTT CTGGGTGAGG 
    AGTAAGAGGA GCAGGCTCCT GCACAGTGAC TACATGAACA TGACTCCCCG CCGCCCCGGG 
    CCCACCCGCA AGCATTACCA GCCCTATGCC CCACCACGCG ACTTCGCAGC CTATCGCTCC 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_006130.1
    CDS59..721
    Translation

    Target ORF information:

    RefSeq Version NM_006139.4
    Organism Homo sapiens(human)
    Definition Homo sapiens CD28 molecule (CD28), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_006139.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCTCAGGC TGCTCTTGGC TCTCAACTTA TTCCCTTCAA TTCAAGTAAC AGGAAACAAG 
    ATTTTGGTGA AGCAGTCGCC CATGCTTGTA GCGTACGACA ATGCGGTCAA CCTTAGCTGC 
    AAGTATTCCT ACAATCTCTT CTCAAGGGAG TTCCGGGCAT CCCTTCACAA AGGACTGGAT 
    AGTGCTGTGG AAGTCTGTGT TGTATATGGG AATTACTCCC AGCAGCTTCA GGTTTACTCA 
    AAAACGGGGT TCAACTGTGA TGGGAAATTG GGCAATGAAT CAGTGACATT CTACCTCCAG 
    AATTTGTATG TTAACCAAAC AGATATTTAC TTCTGCAAAA TTGAAGTTAT GTATCCTCCT 
    CCTTACCTAG ACAATGAGAA GAGCAATGGA ACCATTATCC ATGTGAAAGG GAAACACCTT 
    TGTCCAAGTC CCCTATTTCC CGGACCTTCT AAGCCCTTTT GGGTGCTGGT GGTGGTTGGT 
    GGAGTCCTGG CTTGCTATAG CTTGCTAGTA ACAGTGGCCT TTATTATTTT CTGGGTGAGG 
    AGTAAGAGGA GCAGGCTCCT GCACAGTGAC TACATGAACA TGACTCCCCG CCGCCCCGGG 
    CCCACCCGCA AGCATTACCA GCCCTATGCC CCACCACGCG ACTTCGCAGC CTATCGCTCC 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CD28 ( NM_006139.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006130.1 Homo sapiens CD28 molecule (CD28), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu22476
    Clone ID Related Accession (Same CDS sequence) NM_006139.4 , NM_006139.3
    Accession Version NM_006139.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 663bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1514390400000
    Organism Homo sapiens(human)
    Product T-cell-specific surface glycoprotein CD28 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC125238.5, AK292986.1, J02988.1, DA939711.1, AW241446.1, AA311148.1 and BF509568.1. This sequence is a reference standard in the RefSeqGene project. On Jul 20, 2011 this sequence version replaced NM_006139.2. Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279829.6684.1, J02988.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCTCAGGC TGCTCTTGGC TCTCAACTTA TTCCCTTCAA TTCAAGTAAC AGGAAACAAG 
    ATTTTGGTGA AGCAGTCGCC CATGCTTGTA GCGTACGACA ATGCGGTCAA CCTTAGCTGC 
    AAGTATTCCT ACAATCTCTT CTCAAGGGAG TTCCGGGCAT CCCTTCACAA AGGACTGGAT 
    AGTGCTGTGG AAGTCTGTGT TGTATATGGG AATTACTCCC AGCAGCTTCA GGTTTACTCA 
    AAAACGGGGT TCAACTGTGA TGGGAAATTG GGCAATGAAT CAGTGACATT CTACCTCCAG 
    AATTTGTATG TTAACCAAAC AGATATTTAC TTCTGCAAAA TTGAAGTTAT GTATCCTCCT 
    CCTTACCTAG ACAATGAGAA GAGCAATGGA ACCATTATCC ATGTGAAAGG GAAACACCTT 
    TGTCCAAGTC CCCTATTTCC CGGACCTTCT AAGCCCTTTT GGGTGCTGGT GGTGGTTGGT 
    GGAGTCCTGG CTTGCTATAG CTTGCTAGTA ACAGTGGCCT TTATTATTTT CTGGGTGAGG 
    AGTAAGAGGA GCAGGCTCCT GCACAGTGAC TACATGAACA TGACTCCCCG CCGCCCCGGG 
    CCCACCCGCA AGCATTACCA GCCCTATGCC CCACCACGCG ACTTCGCAGC CTATCGCTCC 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_006130.1
    CDS223..885
    Translation

    Target ORF information:

    RefSeq Version NM_006139.3
    Organism Homo sapiens(human)
    Definition Homo sapiens CD28 molecule (CD28), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_006139.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCTCAGGC TGCTCTTGGC TCTCAACTTA TTCCCTTCAA TTCAAGTAAC AGGAAACAAG 
    ATTTTGGTGA AGCAGTCGCC CATGCTTGTA GCGTACGACA ATGCGGTCAA CCTTAGCTGC 
    AAGTATTCCT ACAATCTCTT CTCAAGGGAG TTCCGGGCAT CCCTTCACAA AGGACTGGAT 
    AGTGCTGTGG AAGTCTGTGT TGTATATGGG AATTACTCCC AGCAGCTTCA GGTTTACTCA 
    AAAACGGGGT TCAACTGTGA TGGGAAATTG GGCAATGAAT CAGTGACATT CTACCTCCAG 
    AATTTGTATG TTAACCAAAC AGATATTTAC TTCTGCAAAA TTGAAGTTAT GTATCCTCCT 
    CCTTACCTAG ACAATGAGAA GAGCAATGGA ACCATTATCC ATGTGAAAGG GAAACACCTT 
    TGTCCAAGTC CCCTATTTCC CGGACCTTCT AAGCCCTTTT GGGTGCTGGT GGTGGTTGGT 
    GGAGTCCTGG CTTGCTATAG CTTGCTAGTA ACAGTGGCCT TTATTATTTT CTGGGTGAGG 
    AGTAAGAGGA GCAGGCTCCT GCACAGTGAC TACATGAACA TGACTCCCCG CCGCCCCGGG 
    CCCACCCGCA AGCATTACCA GCCCTATGCC CCACCACGCG ACTTCGCAGC CTATCGCTCC 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CD28 ( NM_001243078.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001230007.1 Homo sapiens CD28 molecule (CD28), transcript variant 3, mRNA.
    Price & Availability↑
    CloneID OHu07814
    Clone ID Related Accession (Same CDS sequence) NM_001243078.1
    Accession Version NM_001243078.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 306bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1577116800000
    Organism Homo sapiens(human)
    Product T-cell-specific surface glycoprotein CD28 isoform 3 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC125238.5, AF222342.1, AK303421.1, J02988.1, DA939711.1, AW241446.1, AA311148.1 and BF509568.1. Transcript Variant: This variant (3) lacks an in-frame coding exon compared to variant 1. This results in a shorter isoform (3) missing an internal protein segment compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HY256813.1, HY069338.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820687, SAMN01820689 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length. 

    1
    61
    121
    181
    241
    301
    ATGCTCAGGC TGCTCTTGGC TCTCAACTTA TTCCCTTCAA TTCAAGTAAC AGGGAAACAC 
    CTTTGTCCAA GTCCCCTATT TCCCGGACCT TCTAAGCCCT TTTGGGTGCT GGTGGTGGTT 
    GGTGGAGTCC TGGCTTGCTA TAGCTTGCTA GTAACAGTGG CCTTTATTAT TTTCTGGGTG 
    AGGAGTAAGA GGAGCAGGCT CCTGCACAGT GACTACATGA ACATGACTCC CCGCCGCCCC 
    GGGCCCACCC GCAAGCATTA CCAGCCCTAT GCCCCACCAC GCGACTTCGC AGCCTATCGC 
    TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001230007.1
    CDS223..528
    Translation

    Target ORF information:

    RefSeq Version NM_001243078.1
    Organism Homo sapiens(human)
    Definition Homo sapiens CD28 molecule (CD28), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001243078.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    ATGCTCAGGC TGCTCTTGGC TCTCAACTTA TTCCCTTCAA TTCAAGTAAC AGGGAAACAC 
    CTTTGTCCAA GTCCCCTATT TCCCGGACCT TCTAAGCCCT TTTGGGTGCT GGTGGTGGTT 
    GGTGGAGTCC TGGCTTGCTA TAGCTTGCTA GTAACAGTGG CCTTTATTAT TTTCTGGGTG 
    AGGAGTAAGA GGAGCAGGCT CCTGCACAGT GACTACATGA ACATGACTCC CCGCCGCCCC 
    GGGCCCACCC GCAAGCATTA CCAGCCCTAT GCCCCACCAC GCGACTTCGC AGCCTATCGC 
    TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CD28 ( NM_001243077.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001230006.1 Homo sapiens CD28 molecule (CD28), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu07986
    Clone ID Related Accession (Same CDS sequence) NM_001243077.1 , NM_001243077.2
    Accession Version NM_001243077.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 372bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1550764800000
    Organism Homo sapiens(human)
    Product T-cell-specific surface glycoprotein CD28 isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC125238.5, AF222341.1, AK303421.1, J02988.1, DA939711.1, AW241446.1, AA311148.1 and BF509568.1. Transcript Variant: This variant (2) uses an alternate, in-frame donor splice site at one of the coding exons compared to variant 1. This results in a shorter isoform (2) missing an internal protein segment compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR991236.1, AF222341.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length. 

    1
    61
    121
    181
    241
    301
    361
    ATGCTCAGGC TGCTCTTGGC TCTCAACTTA TTCCCTTCAA TTCAAGTAAC AGGAAACAAG 
    ATTTTGGTGA AGCAGTCGCC CATGCTTGTA GCGTACGACA ATGCGGTCAA CCTTAGCTGG 
    AAACACCTTT GTCCAAGTCC CCTATTTCCC GGACCTTCTA AGCCCTTTTG GGTGCTGGTG 
    GTGGTTGGTG GAGTCCTGGC TTGCTATAGC TTGCTAGTAA CAGTGGCCTT TATTATTTTC 
    TGGGTGAGGA GTAAGAGGAG CAGGCTCCTG CACAGTGACT ACATGAACAT GACTCCCCGC 
    CGCCCCGGGC CCACCCGCAA GCATTACCAG CCCTATGCCC CACCACGCGA CTTCGCAGCC 
    TATCGCTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001230006.1
    CDS223..594
    Translation

    Target ORF information:

    RefSeq Version NM_001243077.1
    Organism Homo sapiens(human)
    Definition Homo sapiens CD28 molecule (CD28), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001243077.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    ATGCTCAGGC TGCTCTTGGC TCTCAACTTA TTCCCTTCAA TTCAAGTAAC AGGAAACAAG 
    ATTTTGGTGA AGCAGTCGCC CATGCTTGTA GCGTACGACA ATGCGGTCAA CCTTAGCTGG 
    AAACACCTTT GTCCAAGTCC CCTATTTCCC GGACCTTCTA AGCCCTTTTG GGTGCTGGTG 
    GTGGTTGGTG GAGTCCTGGC TTGCTATAGC TTGCTAGTAA CAGTGGCCTT TATTATTTTC 
    TGGGTGAGGA GTAAGAGGAG CAGGCTCCTG CACAGTGACT ACATGAACAT GACTCCCCGC 
    CGCCCCGGGC CCACCCGCAA GCATTACCAG CCCTATGCCC CACCACGCGA CTTCGCAGCC 
    TATCGCTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CD28 ( NM_001243077.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001230006.1 Homo sapiens CD28 molecule (CD28), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu07986
    Clone ID Related Accession (Same CDS sequence) NM_001243077.1 , NM_001243077.2
    Accession Version NM_001243077.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 372bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1577980800000
    Organism Homo sapiens(human)
    Product T-cell-specific surface glycoprotein CD28 isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC125238.5, AF222341.1, AK303421.1, J02988.1, DA939711.1, AW241446.1, AA311148.1 and BF509568.1. On May 31, 2019 this sequence version replaced NM_001243077.1. Transcript Variant: This variant (2) uses an alternate, in-frame donor splice site at one of the coding exons compared to variant 1. This results in a shorter isoform (2) missing an internal protein segment compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR991236.1, AF222341.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length. 

    1
    61
    121
    181
    241
    301
    361
    ATGCTCAGGC TGCTCTTGGC TCTCAACTTA TTCCCTTCAA TTCAAGTAAC AGGAAACAAG 
    ATTTTGGTGA AGCAGTCGCC CATGCTTGTA GCGTACGACA ATGCGGTCAA CCTTAGCTGG 
    AAACACCTTT GTCCAAGTCC CCTATTTCCC GGACCTTCTA AGCCCTTTTG GGTGCTGGTG 
    GTGGTTGGTG GAGTCCTGGC TTGCTATAGC TTGCTAGTAA CAGTGGCCTT TATTATTTTC 
    TGGGTGAGGA GTAAGAGGAG CAGGCTCCTG CACAGTGACT ACATGAACAT GACTCCCCGC 
    CGCCCCGGGC CCACCCGCAA GCATTACCAG CCCTATGCCC CACCACGCGA CTTCGCAGCC 
    TATCGCTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001230006.1
    CDS59..430
    Translation

    Target ORF information:

    RefSeq Version NM_001243077.2
    Organism Homo sapiens(human)
    Definition Homo sapiens CD28 molecule (CD28), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001243077.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    ATGCTCAGGC TGCTCTTGGC TCTCAACTTA TTCCCTTCAA TTCAAGTAAC AGGAAACAAG 
    ATTTTGGTGA AGCAGTCGCC CATGCTTGTA GCGTACGACA ATGCGGTCAA CCTTAGCTGG 
    AAACACCTTT GTCCAAGTCC CCTATTTCCC GGACCTTCTA AGCCCTTTTG GGTGCTGGTG 
    GTGGTTGGTG GAGTCCTGGC TTGCTATAGC TTGCTAGTAA CAGTGGCCTT TATTATTTTC 
    TGGGTGAGGA GTAAGAGGAG CAGGCTCCTG CACAGTGACT ACATGAACAT GACTCCCCGC 
    CGCCCCGGGC CCACCCGCAA GCATTACCAG CCCTATGCCC CACCACGCGA CTTCGCAGCC 
    TATCGCTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CD28 ( XM_011512194.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510496.1 Homo sapiens CD28 molecule (CD28), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OHu56712
    Clone ID Related Accession (Same CDS sequence) XM_011512194.2
    Accession Version XM_011512194.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 705bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1575475200000
    Organism Homo sapiens(human)
    Product T-cell-specific surface glycoprotein CD28 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512194.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCCTTGTG GTTTGAGTGC CTTGATCATG TGCCCTAAGG GGATGGTGGC GGTGGTGGTG 
    GCCGTGGATG ACGGAGACTC TCAGGCCTTG GCAGGAAACA AGATTTTGGT GAAGCAGTCG 
    CCCATGCTTG TAGCGTACGA CAATGCGGTC AACCTTAGCT GCAAGTATTC CTACAATCTC 
    TTCTCAAGGG AGTTCCGGGC ATCCCTTCAC AAAGGACTGG ATAGTGCTGT GGAAGTCTGT 
    GTTGTATATG GGAATTACTC CCAGCAGCTT CAGGTTTACT CAAAAACGGG GTTCAACTGT 
    GATGGGAAAT TGGGCAATGA ATCAGTGACA TTCTACCTCC AGAATTTGTA TGTTAACCAA 
    ACAGATATTT ACTTCTGCAA AATTGAAGTT ATGTATCCTC CTCCTTACCT AGACAATGAG 
    AAGAGCAATG GAACCATTAT CCATGTGAAA GGGAAACACC TTTGTCCAAG TCCCCTATTT 
    CCCGGACCTT CTAAGCCCTT TTGGGTGCTG GTGGTGGTTG GTGGAGTCCT GGCTTGCTAT 
    AGCTTGCTAG TAACAGTGGC CTTTATTATT TTCTGGGTGA GGAGTAAGAG GAGCAGGCTC 
    CTGCACAGTG ACTACATGAA CATGACTCCC CGCCGCCCCG GGCCCACCCG CAAGCATTAC 
    CAGCCCTATG CCCCACCACG CGACTTCGCA GCCTATCGCT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011510496.1
    CDS16..720
    Translation

    Target ORF information:

    RefSeq Version XM_011512194.2
    Organism Homo sapiens(human)
    Definition Homo sapiens CD28 molecule (CD28), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_011512194.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCCTTGTG GTTTGAGTGC CTTGATCATG TGCCCTAAGG GGATGGTGGC GGTGGTGGTG 
    GCCGTGGATG ACGGAGACTC TCAGGCCTTG GCAGGAAACA AGATTTTGGT GAAGCAGTCG 
    CCCATGCTTG TAGCGTACGA CAATGCGGTC AACCTTAGCT GCAAGTATTC CTACAATCTC 
    TTCTCAAGGG AGTTCCGGGC ATCCCTTCAC AAAGGACTGG ATAGTGCTGT GGAAGTCTGT 
    GTTGTATATG GGAATTACTC CCAGCAGCTT CAGGTTTACT CAAAAACGGG GTTCAACTGT 
    GATGGGAAAT TGGGCAATGA ATCAGTGACA TTCTACCTCC AGAATTTGTA TGTTAACCAA 
    ACAGATATTT ACTTCTGCAA AATTGAAGTT ATGTATCCTC CTCCTTACCT AGACAATGAG 
    AAGAGCAATG GAACCATTAT CCATGTGAAA GGGAAACACC TTTGTCCAAG TCCCCTATTT 
    CCCGGACCTT CTAAGCCCTT TTGGGTGCTG GTGGTGGTTG GTGGAGTCCT GGCTTGCTAT 
    AGCTTGCTAG TAACAGTGGC CTTTATTATT TTCTGGGTGA GGAGTAAGAG GAGCAGGCTC 
    CTGCACAGTG ACTACATGAA CATGACTCCC CGCCGCCCCG GGCCCACCCG CAAGCATTAC 
    CAGCCCTATG CCCCACCACG CGACTTCGCA GCCTATCGCT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CD28 ( XM_011512195.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510497.1 Homo sapiens CD28 molecule (CD28), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OHu56713
    Clone ID Related Accession (Same CDS sequence) XM_011512195.3 , XM_011512195.2
    Accession Version XM_011512195.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1575475200000
    Organism Homo sapiens(human)
    Product T-cell-specific surface glycoprotein CD28 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011512195.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    ATGCCTTGTG GTTTGAGTGC CTTGATCATG TGCCCTAAGG GGATGGTGGC GGTGGTGGTG 
    GCCGTGGATG ACGGAGACTC TCAGGCCTTG GCAGGAAACA AGATTTTGGT GAAGCAGTCG 
    CCCATGCTTG TAGCGTACGA CAATGCGGTC AACCTTAGCT ACAATGAGAA GAGCAATGGA 
    ACCATTATCC ATGTGAAAGG GAAACACCTT TGTCCAAGTC CCCTATTTCC CGGACCTTCT 
    AAGCCCTTTT GGGTGCTGGT GGTGGTTGGT GGAGTCCTGG CTTGCTATAG CTTGCTAGTA 
    ACAGTGGCCT TTATTATTTT CTGGGTGAGG AGTAAGAGGA GCAGGCTCCT GCACAGTGAC 
    TACATGAACA TGACTCCCCG CCGCCCCGGG CCCACCCGCA AGCATTACCA GCCCTATGCC 
    CCACCACGCG ACTTCGCAGC CTATCGCTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011510497.1
    CDS11..463
    Translation

    Target ORF information:

    RefSeq Version XM_011512195.3
    Organism Homo sapiens(human)
    Definition Homo sapiens CD28 molecule (CD28), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_011512195.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    ATGCCTTGTG GTTTGAGTGC CTTGATCATG TGCCCTAAGG GGATGGTGGC GGTGGTGGTG 
    GCCGTGGATG ACGGAGACTC TCAGGCCTTG GCAGGAAACA AGATTTTGGT GAAGCAGTCG 
    CCCATGCTTG TAGCGTACGA CAATGCGGTC AACCTTAGCT ACAATGAGAA GAGCAATGGA 
    ACCATTATCC ATGTGAAAGG GAAACACCTT TGTCCAAGTC CCCTATTTCC CGGACCTTCT 
    AAGCCCTTTT GGGTGCTGGT GGTGGTTGGT GGAGTCCTGG CTTGCTATAG CTTGCTAGTA 
    ACAGTGGCCT TTATTATTTT CTGGGTGAGG AGTAAGAGGA GCAGGCTCCT GCACAGTGAC 
    TACATGAACA TGACTCCCCG CCGCCCCGGG CCCACCCGCA AGCATTACCA GCCCTATGCC 
    CCACCACGCG ACTTCGCAGC CTATCGCTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CD28 ( XM_011512195.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510497.1 Homo sapiens CD28 molecule (CD28), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OHu56713
    Clone ID Related Accession (Same CDS sequence) XM_011512195.3 , XM_011512195.2
    Accession Version XM_011512195.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1465056000000
    Organism Homo sapiens(human)
    Product T-cell-specific surface glycoprotein CD28 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512195.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    ATGCCTTGTG GTTTGAGTGC CTTGATCATG TGCCCTAAGG GGATGGTGGC GGTGGTGGTG 
    GCCGTGGATG ACGGAGACTC TCAGGCCTTG GCAGGAAACA AGATTTTGGT GAAGCAGTCG 
    CCCATGCTTG TAGCGTACGA CAATGCGGTC AACCTTAGCT ACAATGAGAA GAGCAATGGA 
    ACCATTATCC ATGTGAAAGG GAAACACCTT TGTCCAAGTC CCCTATTTCC CGGACCTTCT 
    AAGCCCTTTT GGGTGCTGGT GGTGGTTGGT GGAGTCCTGG CTTGCTATAG CTTGCTAGTA 
    ACAGTGGCCT TTATTATTTT CTGGGTGAGG AGTAAGAGGA GCAGGCTCCT GCACAGTGAC 
    TACATGAACA TGACTCCCCG CCGCCCCGGG CCCACCCGCA AGCATTACCA GCCCTATGCC 
    CCACCACGCG ACTTCGCAGC CTATCGCTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011510497.1
    CDS10..462
    Translation

    Target ORF information:

    RefSeq Version XM_011512195.2
    Organism Homo sapiens(human)
    Definition Homo sapiens CD28 molecule (CD28), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_011512195.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    ATGCCTTGTG GTTTGAGTGC CTTGATCATG TGCCCTAAGG GGATGGTGGC GGTGGTGGTG 
    GCCGTGGATG ACGGAGACTC TCAGGCCTTG GCAGGAAACA AGATTTTGGT GAAGCAGTCG 
    CCCATGCTTG TAGCGTACGA CAATGCGGTC AACCTTAGCT ACAATGAGAA GAGCAATGGA 
    ACCATTATCC ATGTGAAAGG GAAACACCTT TGTCCAAGTC CCCTATTTCC CGGACCTTCT 
    AAGCCCTTTT GGGTGCTGGT GGTGGTTGGT GGAGTCCTGG CTTGCTATAG CTTGCTAGTA 
    ACAGTGGCCT TTATTATTTT CTGGGTGAGG AGTAAGAGGA GCAGGCTCCT GCACAGTGAC 
    TACATGAACA TGACTCCCCG CCGCCCCGGG CCCACCCGCA AGCATTACCA GCCCTATGCC 
    CCACCACGCG ACTTCGCAGC CTATCGCTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CD28 ( XM_011512197.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510499.1 Homo sapiens CD28 molecule (CD28), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OHu56715
    Clone ID Related Accession (Same CDS sequence) XM_011512197.2
    Accession Version XM_011512197.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 411bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1575475200000
    Organism Homo sapiens(human)
    Product T-cell-specific surface glycoprotein CD28 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512197.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    ATGCTCAGGC TGCTCTTGGC TCTCAACTTA TTCCCTTCAA TTCAAGTAAC AGGAAACAAG 
    ATTTTGGTGA AGCAGTCGCC CATGCTTGTA GCGTACGACA ATGCGGTCAA CCTTAGCTAC 
    AATGAGAAGA GCAATGGAAC CATTATCCAT GTGAAAGGGA AACACCTTTG TCCAAGTCCC 
    CTATTTCCCG GACCTTCTAA GCCCTTTTGG GTGCTGGTGG TGGTTGGTGG AGTCCTGGCT 
    TGCTATAGCT TGCTAGTAAC AGTGGCCTTT ATTATTTTCT GGGTGAGGAG TAAGAGGAGC 
    AGGCTCCTGC ACAGTGACTA CATGAACATG ACTCCCCGCC GCCCCGGGCC CACCCGCAAG 
    CATTACCAGC CCTATGCCCC ACCACGCGAC TTCGCAGCCT ATCGCTCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011510499.1
    CDS74..484
    Translation

    Target ORF information:

    RefSeq Version XM_011512197.2
    Organism Homo sapiens(human)
    Definition Homo sapiens CD28 molecule (CD28), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_011512197.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    ATGCTCAGGC TGCTCTTGGC TCTCAACTTA TTCCCTTCAA TTCAAGTAAC AGGAAACAAG 
    ATTTTGGTGA AGCAGTCGCC CATGCTTGTA GCGTACGACA ATGCGGTCAA CCTTAGCTAC 
    AATGAGAAGA GCAATGGAAC CATTATCCAT GTGAAAGGGA AACACCTTTG TCCAAGTCCC 
    CTATTTCCCG GACCTTCTAA GCCCTTTTGG GTGCTGGTGG TGGTTGGTGG AGTCCTGGCT 
    TGCTATAGCT TGCTAGTAAC AGTGGCCTTT ATTATTTTCT GGGTGAGGAG TAAGAGGAGC 
    AGGCTCCTGC ACAGTGACTA CATGAACATG ACTCCCCGCC GCCCCGGGCC CACCCGCAAG 
    CATTACCAGC CCTATGCCCC ACCACGCGAC TTCGCAGCCT ATCGCTCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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