NSMAF cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NSMAF
Entrez Gene ID	8439
Full Name	neutral sphingomyelinase activation associated factor
Synonyms	FAN,GRAMD5
General protein information	Preferred Names neutral sphingomyelinase activation associated factor Names protein FAN factor associated with N-SMase activation factor associated with neutral sphingomyelinase activation neutral sphingomyelinase (N-SMase) activation associated factor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8q12.1
Summary	This gene encodes a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009].
Disorder MIM:	603043

mRNA and Protein(s)

mRNA	Protein	Name
NM_001144772.1	NP_001138244.1	protein FAN isoform 2
NM_003580.3	NP_003571.2	protein FAN isoform 1
NM_003580.4	NP_003571.2	protein FAN isoform 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NSMAF	XP_002692713.2
Homo sapiens (human)	NSMAF	NP_003571.2
Pan troglodytes (chimpanzee)	NSMAF	XP_001156895.1
Mus musculus (house mouse)	Nsmaf	NP_035075.2
Rattus norvegicus (Norway rat)	Nsmaf	NP_852054.1
Canis lupus familiaris (dog)	NSMAF	XP_005638205.1
Danio rerio (zebrafish)	nsmaf	XP_003199050.1
Macaca mulatta (Rhesus monkey)	NSMAF	XP_001088417.1
Gallus gallus (chicken)	NSMAF	XP_003640887.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000429	XP_310670.5
Xenopus tropicalis (tropical clawed frog)	nsmaf	NP_001121464.1

Gene Ontology
Bibliography

Related articles in PubMed

Caspase-2 associates with FAN through direct interaction and overlapping functionality.
Forsberg J, Li X, Zamaraev AV, Panaretakis T, Zhivotovsky B, Olsson M
Biochemical and biophysical research communications499(4)822-828(2018 05)

New variant identified in major susceptibility locus to tuberculosis on chromosomal region 8q12-q13 in Moroccan population: a case control study.
Qrafli M, Asekkaj I, Bourkadi JE, El Aouad R, Sadki K
BMC infectious diseases17(1)712(2017 11)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

The BEACH Domain Protein SPIRRIG Is Essential for Arabidopsis Salt Stress Tolerance and Functions as a Regulator of Transcript Stabilization and Localization.
Steffens A, Bräutigam A, Jakoby M, Hülskamp M
PLoS biology13(7)e1002188(2015 Jul)

Loci associated with N-glycosylation of human immunoglobulin G show pleiotropy with autoimmune diseases and haematological cancers.
Lauc G, Huffman JE, Pučić M, Zgaga L, Adamczyk B, Mužinić A, Novokmet M, Polašek O, Gornik O, Krištić J, Keser T, Vitart V, Scheijen B, Uh HW, Molokhia M, Patrick AL, McKeigue P, Kolčić I, Lukić IK, Swann O, van Leeuwen FN, Ruhaak LR, Houwing-Duistermaat JJ, Slagboom PE, Beekman M, de Craen AJ, Deelder AM, Zeng Q, Wang W, Hastie ND, Gyllensten U, Wilson JF, Wuhrer M, Wright AF, Rudd PM, Hayward C, Aulchenko Y, Campbell H, Rudan I
PLoS genetics9(1)e1003225(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NSMAF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NSMAF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01640	NM_003580.4 Latest version!	Homo sapiens neutral sphingomyelinase activation associated factor (NSMAF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu01454	NM_001144772.1 Latest version!	Homo sapiens neutral sphingomyelinase activation associated factor (NSMAF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$475.30 $679.00
View Old Accession Versions >>
OHu01640	NM_003580.3	Homo sapiens neutral sphingomyelinase activation associated factor (NSMAF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NSMAF ( NM_003580.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003571.2 Homo sapiens neutral sphingomyelinase activation associated factor (NSMAF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01640
Clone ID Related Accession (Same CDS sequence)	NM_003580.3 , NM_003580.4
Accession Version	NM_003580.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511712000000
Organism	Homo sapiens(human)
Product	protein FAN isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR005814.1, BC041124.1 and AC068522.7. On Jan 27, 2009 this sequence version replaced NM_003580.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the shorter isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC041124.1, SRR1163658.247133.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTTTA TCCGGAAGAA GCAGCAGGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGA 
TTTTCCTTGC TGCTGCTTAA CTTGGAGGAG TACTACTTTG AACAGCATAG AGCCAATCAC 
ATTTTGCACA AGGGCAGTCA CCATGAAAGG AAAATCAGAG GCTCCTTAAA AATATGTTCA 
AAATCGGTGA TTTTTGAACC AGATTCAATA TCCCAGCCCA TCATCAAGAT TCCTTTGAGA 
GACTGTATAA AAATAGGAAA GCATGGAGAA AATGGAGCCA ATAGACACTT CACAAAGGCA 
AAATCTGGGG GTATTTCACT CATTTTCAGT CAGGTATATT TCATTAAAGA ACATAATGTT 
GTTGCACCAT ATAAAATAGA AAGGGGCAAA ATGGAATATG TTTTTGAATT GGATGTTCCC 
GGGAAAGTGG AAGATGTTGT GGAGACGTTG CTTCAGCTTC ACAGAGCATC CTGCCTTGAC 
AAATTGGGTG ACCAAACCGC CATGATAACA GCTATTTTGC AGTCTCGTTT AGCTAGAACA 
TCATTTGACA AAAACAGGTT CCAAAACATT TCTGAAAAGC TGCACATGGA ATGCAAAGCA 
GAAATGGTGA CGCCTCTGGT GACTAATCCT GGACACGTGT GCATCACGGA CACAAACCTG 
TATTTTCAGC CCCTCAACGG CTACCCGAAA CCTGTGGTCC AGATAACACT CCAAGATGTC 
CGCCGCATCT ACAAAAGGAG GCACGGCCTC ATGCCTCTGG GCTTGGAAGT ATTTTGCACA 
GAAGATGATC TGTGTTCCGA CATCTACCTA AAGTTCTATG AACCTCAAGA TAGAGATGAT 
CTCTATTTTT ACATTGCCAC ATACCTAGAG CACCATGTGG CGGAGCACAC TGCTGAGAGC 
TACATGCTGC AGTGGCAGCG TGGACACCTT TCCAACTATC AGTACCTCCT TCACCTCAAC 
AACCTGGCCG ACCGCAGCTG CAACGACCTC TCCCAGTACC CTGTGTTTCC ATGGATAATA 
CATGATTATT CCAGCTCAGA ACTAGATTTG TCAAATCCAG GAACCTTCCG GGATCTCAGT 
AAGCCAGTAG GGGCCCTAAA TAAGGAACGG CTGGAGAGAC TACTGACACG CTACCAGGAA 
ATGCCTGAAC CAAAGTTCAT GTATGGGAGT CACTACTCTT CCCCGGGTTA TGTACTTTTT 
TATCTTGTTA GGATTGCACC AGAGTATATG CTGTGCCTGC AGAATGGAAG ATTTGATAAT 
GCAGATAGAA TGTTCAACAG TATTGCAGAA ACTTGGAAAA ACTGTCTGGA TGGTGCAACG 
GATTTTAAAG AGTTAATTCC AGAATTCTAT GGTGATGATG TGAGCTTTCT AGTCAATAGC 
CTGAAGTTGG ATTTGGGAAA GAGACAAGGA GGACAGATGG TTGACGACGT GGAGCTTCCC 
CCTTGGGCTT CCAGTCCCGA GGACTTTCTC CAGAAGAGCA AAGATGCATT GGAAAGCAAT 
TATGTGTCTG AACACCTTCA CGAGTGGATT GATCTAATAT TTGGCTACAA ACAAAAAGGG 
AGTGATGCAG TTGGGGCCCA TAATGTATTT CATCCCCTGA CCTATGAAGG AGGTGTAGAC 
TTGAACAGCA TCCAGGATCC TGATGAGAAG GTAGCCATGC TTACGCAAAT CTTGGAATTT 
GGGCAGACAC CAAAACAACT ATTTGTGACA CCACATCCTC GAAGGATCAC CCCAAAGTTT 
AAAAGTTTGT CCCAGACCTC CAGTTATAAT GCTTCTATGG CAGATTCCCC AGGTGAAGAG 
TCTTTTGAAG ACCTGACCGA AGAAAGCAAA ACACTGGCCT GGAATAACAT CACCAAACTG 
CAGTTACACG AGCACTATAA AATCCACAAA GAAGCAGTTA CTGGAATCAC GGTCTCTCGC 
AATGGATCTT CAGTATTCAC AACATCCCAA GATTCCACCT TGAAGATGTT TTCTAAAGAA 
TCAAAAATGC TACAAAGAAG TATATCATTT TCAAATATGG CTTTATCGTC TTGTTTACTT 
TTACCAGGAG ATGCCACTGT CATAACTTCT TCATGGGATA ATAATGTCTA TTTTTATTCC 
ATAGCATTTG GAAGACGCCA GGACACGTTA ATGGGACATG ATGATGCTGT TAGTAAGATC 
TGTTGGCATG ACAACAGGCT ATATTCTGCA TCGTGGGACT CTACAGTGAA GGTGTGGTCT 
GGTGTTCCTG CAGAGATGCC AGGCACCAAA AGACACCACT TTGACTTGCT GGCCGAGCTG 
GAACATGATG TCAGTGTAGA TACAATCAGT TTAAATGCTG CAAGCACACT GTTAGTTTCC 
GGCACCAAAG AAGGCACAGT GAATATTTGG GACCTCACAA CGGCCACCTT AATGCACCAG 
ATTCCATGCC ATTCAGGGAT TGTATGTGAC ACTGCTTTTA GCCCAGATAG TCGCCATGTC 
CTCAGCACAG GAACAGATGG CTGTCTTAAT GTCATTGATG TGCAGACAGG AATGCTCATC 
TCCTCCATGA CATCAGATGA GCCCCAGAGG TGCTTTGTCT GGGATGGAAA TTCCGTTTTA 
TCTGGCAGTC AGTCTGGTGA ACTGCTCGTT TGGGACCTCC TTGGAGCAAA AATCAGTGAG 
AGAATACAGG GCCACACAGG TGCTGTGACA TGTATATGGA TGAATGAACA GTGTAGCAGT 
ATCATCACAG GAGGGGAAGA CAGACAAATT ATATTCTGGA AATTGCAGTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003571.2
CDS	215..2968
Translation	MAFIRKKQQEQQLQLYSKERFSLLLLNLEEYYFEQHRANHILHKGSHHERKIRGSLKICSKSVIFEPDSISQPIIKIPLRDCIKIGKHGENGANRHFTKAKSGGISLIFSQVYFIKEHNVVAPYKIERGKMEYVFELDVPGKVEDVVETLLQLHRASCLDKLGDQTAMITAILQSRLARTSFDKNRFQNISEKLHMECKAEMVTPLVTNPGHVCITDTNLYFQPLNGYPKPVVQITLQDVRRIYKRRHGLMPLGLEVFCTEDDLCSDIYLKFYEPQDRDDLYFYIATYLEHHVAEHTAESYMLQWQRGHLSNYQYLLHLNNLADRSCNDLSQYPVFPWIIHDYSSSELDLSNPGTFRDLSKPVGALNKERLERLLTRYQEMPEPKFMYGSHYSSPGYVLFYLVRIAPEYMLCLQNGRFDNADRMFNSIAETWKNCLDGATDFKELIPEFYGDDVSFLVNSLKLDLGKRQGGQMVDDVELPPWASSPEDFLQKSKDALESNYVSEHLHEWIDLIFGYKQKGSDAVGAHNVFHPLTYEGGVDLNSIQDPDEKVAMLTQILEFGQTPKQLFVTPHPRRITPKFKSLSQTSSYNASMADSPGEESFEDLTEESKTLAWNNITKLQLHEHYKIHKEAVTGITVSRNGSSVFTTSQDSTLKMFSKESKMLQRSISFSNMALSSCLLLPGDATVITSSWDNNVYFYSIAFGRRQDTLMGHDDAVSKICWHDNRLYSASWDSTVKVWSGVPAEMPGTKRHHFDLLAELEHDVSVDTISLNAASTLLVSGTKEGTVNIWDLTTATLMHQIPCHSGIVCDTAFSPDSRHVLSTGTDGCLNVIDVQTGMLISSMTSDEPQRCFVWDGNSVLSGSQSGELLVWDLLGAKISERIQGHTGAVTCIWMNEQCSSIITGGEDRQIIFWKLQY

Target ORF information:

RefSeq Version	NM_003580.3
Organism	Homo sapiens(human)
Definition	Homo sapiens neutral sphingomyelinase activation associated factor (NSMAF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003580.3

ORF Insert Sequence:

ATGGCGTTTA TCCGGAAGAA GCAGCAGGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGA 
TTTTCCTTGC TGCTGCTTAA CTTGGAGGAG TACTACTTTG AACAGCATAG AGCCAATCAC 
ATTTTGCACA AGGGCAGTCA CCATGAAAGG AAAATCAGAG GCTCCTTAAA AATATGTTCA 
AAATCGGTGA TTTTTGAACC AGATTCAATA TCCCAGCCCA TCATCAAGAT TCCTTTGAGA 
GACTGTATAA AAATAGGAAA GCATGGAGAA AATGGAGCCA ATAGACACTT CACAAAGGCA 
AAATCTGGGG GTATTTCACT CATTTTCAGT CAGGTATATT TCATTAAAGA ACATAATGTT 
GTTGCACCAT ATAAAATAGA AAGGGGCAAA ATGGAATATG TTTTTGAATT GGATGTTCCC 
GGGAAAGTGG AAGATGTTGT GGAGACGTTG CTTCAGCTTC ACAGAGCATC CTGCCTTGAC 
AAATTGGGTG ACCAAACCGC CATGATAACA GCTATTTTGC AGTCTCGTTT AGCTAGAACA 
TCATTTGACA AAAACAGGTT CCAAAACATT TCTGAAAAGC TGCACATGGA ATGCAAAGCA 
GAAATGGTGA CGCCTCTGGT GACTAATCCT GGACACGTGT GCATCACGGA CACAAACCTG 
TATTTTCAGC CCCTCAACGG CTACCCGAAA CCTGTGGTCC AGATAACACT CCAAGATGTC 
CGCCGCATCT ACAAAAGGAG GCACGGCCTC ATGCCTCTGG GCTTGGAAGT ATTTTGCACA 
GAAGATGATC TGTGTTCCGA CATCTACCTA AAGTTCTATG AACCTCAAGA TAGAGATGAT 
CTCTATTTTT ACATTGCCAC ATACCTAGAG CACCATGTGG CGGAGCACAC TGCTGAGAGC 
TACATGCTGC AGTGGCAGCG TGGACACCTT TCCAACTATC AGTACCTCCT TCACCTCAAC 
AACCTGGCCG ACCGCAGCTG CAACGACCTC TCCCAGTACC CTGTGTTTCC ATGGATAATA 
CATGATTATT CCAGCTCAGA ACTAGATTTG TCAAATCCAG GAACCTTCCG GGATCTCAGT 
AAGCCAGTAG GGGCCCTAAA TAAGGAACGG CTGGAGAGAC TACTGACACG CTACCAGGAA 
ATGCCTGAAC CAAAGTTCAT GTATGGGAGT CACTACTCTT CCCCGGGTTA TGTACTTTTT 
TATCTTGTTA GGATTGCACC AGAGTATATG CTGTGCCTGC AGAATGGAAG ATTTGATAAT 
GCAGATAGAA TGTTCAACAG TATTGCAGAA ACTTGGAAAA ACTGTCTGGA TGGTGCAACG 
GATTTTAAAG AGTTAATTCC AGAATTCTAT GGTGATGATG TGAGCTTTCT AGTCAATAGC 
CTGAAGTTGG ATTTGGGAAA GAGACAAGGA GGACAGATGG TTGACGACGT GGAGCTTCCC 
CCTTGGGCTT CCAGTCCCGA GGACTTTCTC CAGAAGAGCA AAGATGCATT GGAAAGCAAT 
TATGTGTCTG AACACCTTCA CGAGTGGATT GATCTAATAT TTGGCTACAA ACAAAAAGGG 
AGTGATGCAG TTGGGGCCCA TAATGTATTT CATCCCCTGA CCTATGAAGG AGGTGTAGAC 
TTGAACAGCA TCCAGGATCC TGATGAGAAG GTAGCCATGC TTACGCAAAT CTTGGAATTT 
GGGCAGACAC CAAAACAACT ATTTGTGACA CCACATCCTC GAAGGATCAC CCCAAAGTTT 
AAAAGTTTGT CCCAGACCTC CAGTTATAAT GCTTCTATGG CAGATTCCCC AGGTGAAGAG 
TCTTTTGAAG ACCTGACCGA AGAAAGCAAA ACACTGGCCT GGAATAACAT CACCAAACTG 
CAGTTACACG AGCACTATAA AATCCACAAA GAAGCAGTTA CTGGAATCAC GGTCTCTCGC 
AATGGATCTT CAGTATTCAC AACATCCCAA GATTCCACCT TGAAGATGTT TTCTAAAGAA 
TCAAAAATGC TACAAAGAAG TATATCATTT TCAAATATGG CTTTATCGTC TTGTTTACTT 
TTACCAGGAG ATGCCACTGT CATAACTTCT TCATGGGATA ATAATGTCTA TTTTTATTCC 
ATAGCATTTG GAAGACGCCA GGACACGTTA ATGGGACATG ATGATGCTGT TAGTAAGATC 
TGTTGGCATG ACAACAGGCT ATATTCTGCA TCGTGGGACT CTACAGTGAA GGTGTGGTCT 
GGTGTTCCTG CAGAGATGCC AGGCACCAAA AGACACCACT TTGACTTGCT GGCCGAGCTG 
GAACATGATG TCAGTGTAGA TACAATCAGT TTAAATGCTG CAAGCACACT GTTAGTTTCC 
GGCACCAAAG AAGGCACAGT GAATATTTGG GACCTCACAA CGGCCACCTT AATGCACCAG 
ATTCCATGCC ATTCAGGGAT TGTATGTGAC ACTGCTTTTA GCCCAGATAG TCGCCATGTC 
CTCAGCACAG GAACAGATGG CTGTCTTAAT GTCATTGATG TGCAGACAGG AATGCTCATC 
TCCTCCATGA CATCAGATGA GCCCCAGAGG TGCTTTGTCT GGGATGGAAA TTCCGTTTTA 
TCTGGCAGTC AGTCTGGTGA ACTGCTCGTT TGGGACCTCC TTGGAGCAAA AATCAGTGAG 
AGAATACAGG GCCACACAGG TGCTGTGACA TGTATATGGA TGAATGAACA GTGTAGCAGT 
ATCATCACAG GAGGGGAAGA CAGACAAATT ATATTCTGGA AATTGCAGTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSMAF ( NM_003580.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003571.2 Homo sapiens neutral sphingomyelinase activation associated factor (NSMAF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01640
Clone ID Related Accession (Same CDS sequence)	NM_003580.3 , NM_003580.4
Accession Version	NM_003580.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570550400000
Organism	Homo sapiens(human)
Product	protein FAN isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092700.2, DR005814.1, BC041124.1 and AC068522.7. On Nov 23, 2018 this sequence version replaced NM_003580.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the shorter isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X96586.1, BC041124.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000038176.8/ ENSP00000038176.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGTTTA TCCGGAAGAA GCAGCAGGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGA 
TTTTCCTTGC TGCTGCTTAA CTTGGAGGAG TACTACTTTG AACAGCATAG AGCCAATCAC 
ATTTTGCACA AGGGCAGTCA CCATGAAAGG AAAATCAGAG GCTCCTTAAA AATATGTTCA 
AAATCGGTGA TTTTTGAACC AGATTCAATA TCCCAGCCCA TCATCAAGAT TCCTTTGAGA 
GACTGTATAA AAATAGGAAA GCATGGAGAA AATGGAGCCA ATAGACACTT CACAAAGGCA 
AAATCTGGGG GTATTTCACT CATTTTCAGT CAGGTATATT TCATTAAAGA ACATAATGTT 
GTTGCACCAT ATAAAATAGA AAGGGGCAAA ATGGAATATG TTTTTGAATT GGATGTTCCC 
GGGAAAGTGG AAGATGTTGT GGAGACGTTG CTTCAGCTTC ACAGAGCATC CTGCCTTGAC 
AAATTGGGTG ACCAAACCGC CATGATAACA GCTATTTTGC AGTCTCGTTT AGCTAGAACA 
TCATTTGACA AAAACAGGTT CCAAAACATT TCTGAAAAGC TGCACATGGA ATGCAAAGCA 
GAAATGGTGA CGCCTCTGGT GACTAATCCT GGACACGTGT GCATCACGGA CACAAACCTG 
TATTTTCAGC CCCTCAACGG CTACCCGAAA CCTGTGGTCC AGATAACACT CCAAGATGTC 
CGCCGCATCT ACAAAAGGAG GCACGGCCTC ATGCCTCTGG GCTTGGAAGT ATTTTGCACA 
GAAGATGATC TGTGTTCCGA CATCTACCTA AAGTTCTATG AACCTCAAGA TAGAGATGAT 
CTCTATTTTT ACATTGCCAC ATACCTAGAG CACCATGTGG CGGAGCACAC TGCTGAGAGC 
TACATGCTGC AGTGGCAGCG TGGACACCTT TCCAACTATC AGTACCTCCT TCACCTCAAC 
AACCTGGCCG ACCGCAGCTG CAACGACCTC TCCCAGTACC CTGTGTTTCC ATGGATAATA 
CATGATTATT CCAGCTCAGA ACTAGATTTG TCAAATCCAG GAACCTTCCG GGATCTCAGT 
AAGCCAGTAG GGGCCCTAAA TAAGGAACGG CTGGAGAGAC TACTGACACG CTACCAGGAA 
ATGCCTGAAC CAAAGTTCAT GTATGGGAGT CACTACTCTT CCCCGGGTTA TGTACTTTTT 
TATCTTGTTA GGATTGCACC AGAGTATATG CTGTGCCTGC AGAATGGAAG ATTTGATAAT 
GCAGATAGAA TGTTCAACAG TATTGCAGAA ACTTGGAAAA ACTGTCTGGA TGGTGCAACG 
GATTTTAAAG AGTTAATTCC AGAATTCTAT GGTGATGATG TGAGCTTTCT AGTCAATAGC 
CTGAAGTTGG ATTTGGGAAA GAGACAAGGA GGACAGATGG TTGACGACGT GGAGCTTCCC 
CCTTGGGCTT CCAGTCCCGA GGACTTTCTC CAGAAGAGCA AAGATGCATT GGAAAGCAAT 
TATGTGTCTG AACACCTTCA CGAGTGGATT GATCTAATAT TTGGCTACAA ACAAAAAGGG 
AGTGATGCAG TTGGGGCCCA TAATGTATTT CATCCCCTGA CCTATGAAGG AGGTGTAGAC 
TTGAACAGCA TCCAGGATCC TGATGAGAAG GTAGCCATGC TTACGCAAAT CTTGGAATTT 
GGGCAGACAC CAAAACAACT ATTTGTGACA CCACATCCTC GAAGGATCAC CCCAAAGTTT 
AAAAGTTTGT CCCAGACCTC CAGTTATAAT GCTTCTATGG CAGATTCCCC AGGTGAAGAG 
TCTTTTGAAG ACCTGACCGA AGAAAGCAAA ACACTGGCCT GGAATAACAT CACCAAACTG 
CAGTTACACG AGCACTATAA AATCCACAAA GAAGCAGTTA CTGGAATCAC GGTCTCTCGC 
AATGGATCTT CAGTATTCAC AACATCCCAA GATTCCACCT TGAAGATGTT TTCTAAAGAA 
TCAAAAATGC TACAAAGAAG TATATCATTT TCAAATATGG CTTTATCGTC TTGTTTACTT 
TTACCAGGAG ATGCCACTGT CATAACTTCT TCATGGGATA ATAATGTCTA TTTTTATTCC 
ATAGCATTTG GAAGACGCCA GGACACGTTA ATGGGACATG ATGATGCTGT TAGTAAGATC 
TGTTGGCATG ACAACAGGCT ATATTCTGCA TCGTGGGACT CTACAGTGAA GGTGTGGTCT 
GGTGTTCCTG CAGAGATGCC AGGCACCAAA AGACACCACT TTGACTTGCT GGCCGAGCTG 
GAACATGATG TCAGTGTAGA TACAATCAGT TTAAATGCTG CAAGCACACT GTTAGTTTCC 
GGCACCAAAG AAGGCACAGT GAATATTTGG GACCTCACAA CGGCCACCTT AATGCACCAG 
ATTCCATGCC ATTCAGGGAT TGTATGTGAC ACTGCTTTTA GCCCAGATAG TCGCCATGTC 
CTCAGCACAG GAACAGATGG CTGTCTTAAT GTCATTGATG TGCAGACAGG AATGCTCATC 
TCCTCCATGA CATCAGATGA GCCCCAGAGG TGCTTTGTCT GGGATGGAAA TTCCGTTTTA 
TCTGGCAGTC AGTCTGGTGA ACTGCTCGTT TGGGACCTCC TTGGAGCAAA AATCAGTGAG 
AGAATACAGG GCCACACAGG TGCTGTGACA TGTATATGGA TGAATGAACA GTGTAGCAGT 
ATCATCACAG GAGGGGAAGA CAGACAAATT ATATTCTGGA AATTGCAGTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003571.2
CDS	223..2976
Translation	MAFIRKKQQEQQLQLYSKERFSLLLLNLEEYYFEQHRANHILHKGSHHERKIRGSLKICSKSVIFEPDSISQPIIKIPLRDCIKIGKHGENGANRHFTKAKSGGISLIFSQVYFIKEHNVVAPYKIERGKMEYVFELDVPGKVEDVVETLLQLHRASCLDKLGDQTAMITAILQSRLARTSFDKNRFQNISEKLHMECKAEMVTPLVTNPGHVCITDTNLYFQPLNGYPKPVVQITLQDVRRIYKRRHGLMPLGLEVFCTEDDLCSDIYLKFYEPQDRDDLYFYIATYLEHHVAEHTAESYMLQWQRGHLSNYQYLLHLNNLADRSCNDLSQYPVFPWIIHDYSSSELDLSNPGTFRDLSKPVGALNKERLERLLTRYQEMPEPKFMYGSHYSSPGYVLFYLVRIAPEYMLCLQNGRFDNADRMFNSIAETWKNCLDGATDFKELIPEFYGDDVSFLVNSLKLDLGKRQGGQMVDDVELPPWASSPEDFLQKSKDALESNYVSEHLHEWIDLIFGYKQKGSDAVGAHNVFHPLTYEGGVDLNSIQDPDEKVAMLTQILEFGQTPKQLFVTPHPRRITPKFKSLSQTSSYNASMADSPGEESFEDLTEESKTLAWNNITKLQLHEHYKIHKEAVTGITVSRNGSSVFTTSQDSTLKMFSKESKMLQRSISFSNMALSSCLLLPGDATVITSSWDNNVYFYSIAFGRRQDTLMGHDDAVSKICWHDNRLYSASWDSTVKVWSGVPAEMPGTKRHHFDLLAELEHDVSVDTISLNAASTLLVSGTKEGTVNIWDLTTATLMHQIPCHSGIVCDTAFSPDSRHVLSTGTDGCLNVIDVQTGMLISSMTSDEPQRCFVWDGNSVLSGSQSGELLVWDLLGAKISERIQGHTGAVTCIWMNEQCSSIITGGEDRQIIFWKLQY

Target ORF information:

RefSeq Version	NM_003580.4
Organism	Homo sapiens(human)
Definition	Homo sapiens neutral sphingomyelinase activation associated factor (NSMAF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003580.4

ORF Insert Sequence:

ATGGCGTTTA TCCGGAAGAA GCAGCAGGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGA 
TTTTCCTTGC TGCTGCTTAA CTTGGAGGAG TACTACTTTG AACAGCATAG AGCCAATCAC 
ATTTTGCACA AGGGCAGTCA CCATGAAAGG AAAATCAGAG GCTCCTTAAA AATATGTTCA 
AAATCGGTGA TTTTTGAACC AGATTCAATA TCCCAGCCCA TCATCAAGAT TCCTTTGAGA 
GACTGTATAA AAATAGGAAA GCATGGAGAA AATGGAGCCA ATAGACACTT CACAAAGGCA 
AAATCTGGGG GTATTTCACT CATTTTCAGT CAGGTATATT TCATTAAAGA ACATAATGTT 
GTTGCACCAT ATAAAATAGA AAGGGGCAAA ATGGAATATG TTTTTGAATT GGATGTTCCC 
GGGAAAGTGG AAGATGTTGT GGAGACGTTG CTTCAGCTTC ACAGAGCATC CTGCCTTGAC 
AAATTGGGTG ACCAAACCGC CATGATAACA GCTATTTTGC AGTCTCGTTT AGCTAGAACA 
TCATTTGACA AAAACAGGTT CCAAAACATT TCTGAAAAGC TGCACATGGA ATGCAAAGCA 
GAAATGGTGA CGCCTCTGGT GACTAATCCT GGACACGTGT GCATCACGGA CACAAACCTG 
TATTTTCAGC CCCTCAACGG CTACCCGAAA CCTGTGGTCC AGATAACACT CCAAGATGTC 
CGCCGCATCT ACAAAAGGAG GCACGGCCTC ATGCCTCTGG GCTTGGAAGT ATTTTGCACA 
GAAGATGATC TGTGTTCCGA CATCTACCTA AAGTTCTATG AACCTCAAGA TAGAGATGAT 
CTCTATTTTT ACATTGCCAC ATACCTAGAG CACCATGTGG CGGAGCACAC TGCTGAGAGC 
TACATGCTGC AGTGGCAGCG TGGACACCTT TCCAACTATC AGTACCTCCT TCACCTCAAC 
AACCTGGCCG ACCGCAGCTG CAACGACCTC TCCCAGTACC CTGTGTTTCC ATGGATAATA 
CATGATTATT CCAGCTCAGA ACTAGATTTG TCAAATCCAG GAACCTTCCG GGATCTCAGT 
AAGCCAGTAG GGGCCCTAAA TAAGGAACGG CTGGAGAGAC TACTGACACG CTACCAGGAA 
ATGCCTGAAC CAAAGTTCAT GTATGGGAGT CACTACTCTT CCCCGGGTTA TGTACTTTTT 
TATCTTGTTA GGATTGCACC AGAGTATATG CTGTGCCTGC AGAATGGAAG ATTTGATAAT 
GCAGATAGAA TGTTCAACAG TATTGCAGAA ACTTGGAAAA ACTGTCTGGA TGGTGCAACG 
GATTTTAAAG AGTTAATTCC AGAATTCTAT GGTGATGATG TGAGCTTTCT AGTCAATAGC 
CTGAAGTTGG ATTTGGGAAA GAGACAAGGA GGACAGATGG TTGACGACGT GGAGCTTCCC 
CCTTGGGCTT CCAGTCCCGA GGACTTTCTC CAGAAGAGCA AAGATGCATT GGAAAGCAAT 
TATGTGTCTG AACACCTTCA CGAGTGGATT GATCTAATAT TTGGCTACAA ACAAAAAGGG 
AGTGATGCAG TTGGGGCCCA TAATGTATTT CATCCCCTGA CCTATGAAGG AGGTGTAGAC 
TTGAACAGCA TCCAGGATCC TGATGAGAAG GTAGCCATGC TTACGCAAAT CTTGGAATTT 
GGGCAGACAC CAAAACAACT ATTTGTGACA CCACATCCTC GAAGGATCAC CCCAAAGTTT 
AAAAGTTTGT CCCAGACCTC CAGTTATAAT GCTTCTATGG CAGATTCCCC AGGTGAAGAG 
TCTTTTGAAG ACCTGACCGA AGAAAGCAAA ACACTGGCCT GGAATAACAT CACCAAACTG 
CAGTTACACG AGCACTATAA AATCCACAAA GAAGCAGTTA CTGGAATCAC GGTCTCTCGC 
AATGGATCTT CAGTATTCAC AACATCCCAA GATTCCACCT TGAAGATGTT TTCTAAAGAA 
TCAAAAATGC TACAAAGAAG TATATCATTT TCAAATATGG CTTTATCGTC TTGTTTACTT 
TTACCAGGAG ATGCCACTGT CATAACTTCT TCATGGGATA ATAATGTCTA TTTTTATTCC 
ATAGCATTTG GAAGACGCCA GGACACGTTA ATGGGACATG ATGATGCTGT TAGTAAGATC 
TGTTGGCATG ACAACAGGCT ATATTCTGCA TCGTGGGACT CTACAGTGAA GGTGTGGTCT 
GGTGTTCCTG CAGAGATGCC AGGCACCAAA AGACACCACT TTGACTTGCT GGCCGAGCTG 
GAACATGATG TCAGTGTAGA TACAATCAGT TTAAATGCTG CAAGCACACT GTTAGTTTCC 
GGCACCAAAG AAGGCACAGT GAATATTTGG GACCTCACAA CGGCCACCTT AATGCACCAG 
ATTCCATGCC ATTCAGGGAT TGTATGTGAC ACTGCTTTTA GCCCAGATAG TCGCCATGTC 
CTCAGCACAG GAACAGATGG CTGTCTTAAT GTCATTGATG TGCAGACAGG AATGCTCATC 
TCCTCCATGA CATCAGATGA GCCCCAGAGG TGCTTTGTCT GGGATGGAAA TTCCGTTTTA 
TCTGGCAGTC AGTCTGGTGA ACTGCTCGTT TGGGACCTCC TTGGAGCAAA AATCAGTGAG 
AGAATACAGG GCCACACAGG TGCTGTGACA TGTATATGGA TGAATGAACA GTGTAGCAGT 
ATCATCACAG GAGGGGAAGA CAGACAAATT ATATTCTGGA AATTGCAGTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSMAF ( NM_001144772.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001138244.1 Homo sapiens neutral sphingomyelinase activation associated factor (NSMAF), transcript variant 2, mRNA.

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CloneID	OHu01454
Clone ID Related Accession (Same CDS sequence)	NM_001144772.1
Accession Version	NM_001144772.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570550400000
Organism	Homo sapiens(human)
Product	protein FAN isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK294009.1, AC068522.7 and BC041124.1. Transcript Variant: This variant (2) uses an alternate exon in the 5' UTR and coding region. Isoform 2 has a longer N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK294009.1, SRR1660807.201291.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGGCC AGGCCAGATT CCACCGCGGA GCCGAGGCAT GCCGAGCTAT CCGGCAGTGC 
AGGTCCCGGC GGCCGCGGGC AGCTCACCCG GAAGGCCGCG CGGGGTCCGG GCGTGGATCC 
CGGCACGCGA GCCCGGGCGT TCGCAGGGGA GGATTTTCCT TGCTGCTGCT TAACTTGGAG 
GAGTACTACT TTGAACAGCA TAGAGCCAAT CACATTTTGC ACAAGGGCAG TCACCATGAA 
AGGAAAATCA GAGGCTCCTT AAAAATATGT TCAAAATCGG TGATTTTTGA ACCAGATTCA 
ATATCCCAGC CCATCATCAA GATTCCTTTG AGAGACTGTA TAAAAATAGG AAAGCATGGA 
GAAAATGGAG CCAATAGACA CTTCACAAAG GCAAAATCTG GGGGTATTTC ACTCATTTTC 
AGTCAGGTAT ATTTCATTAA AGAACATAAT GTTGTTGCAC CATATAAAAT AGAAAGGGGC 
AAAATGGAAT ATGTTTTTGA ATTGGATGTT CCCGGGAAAG TGGAAGATGT TGTGGAGACG 
TTGCTTCAGC TTCACAGAGC ATCCTGCCTT GACAAATTGG GTGACCAAAC CGCCATGATA 
ACAGCTATTT TGCAGTCTCG TTTAGCTAGA ACATCATTTG ACAAAAACAG GTTCCAAAAC 
ATTTCTGAAA AGCTGCACAT GGAATGCAAA GCAGAAATGG TGACGCCTCT GGTGACTAAT 
CCTGGACACG TGTGCATCAC GGACACAAAC CTGTATTTTC AGCCCCTCAA CGGCTACCCG 
AAACCTGTGG TCCAGATAAC ACTCCAAGAT GTCCGCCGCA TCTACAAAAG GAGGCACGGC 
CTCATGCCTC TGGGCTTGGA AGTATTTTGC ACAGAAGATG ATCTGTGTTC CGACATCTAC 
CTAAAGTTCT ATGAACCTCA AGATAGAGAT GATCTCTATT TTTACATTGC CACATACCTA 
GAGCACCATG TGGCGGAGCA CACTGCTGAG AGCTACATGC TGCAGTGGCA GCGTGGACAC 
CTTTCCAACT ATCAGTACCT CCTTCACCTC AACAACCTGG CCGACCGCAG CTGCAACGAC 
CTCTCCCAGT ACCCTGTGTT TCCATGGATA ATACATGATT ATTCCAGCTC AGAACTAGAT 
TTGTCAAATC CAGGAACCTT CCGGGATCTC AGTAAGCCAG TAGGGGCCCT AAATAAGGAA 
CGGCTGGAGA GACTACTGAC ACGCTACCAG GAAATGCCTG AACCAAAGTT CATGTATGGG 
AGTCACTACT CTTCCCCGGG TTATGTACTT TTTTATCTTG TTAGGATTGC ACCAGAGTAT 
ATGCTGTGCC TGCAGAATGG AAGATTTGAT AATGCAGATA GAATGTTCAA CAGTATTGCA 
GAAACTTGGA AAAACTGTCT GGATGGTGCA ACGGATTTTA AAGAGTTAAT TCCAGAATTC 
TATGGTGATG ATGTGAGCTT TCTAGTCAAT AGCCTGAAGT TGGATTTGGG AAAGAGACAA 
GGAGGACAGA TGGTTGACGA CGTGGAGCTT CCCCCTTGGG CTTCCAGTCC CGAGGACTTT 
CTCCAGAAGA GCAAAGATGC ATTGGAAAGC AATTATGTGT CTGAACACCT TCACGAGTGG 
ATTGATCTAA TATTTGGCTA CAAACAAAAA GGGAGTGATG CAGTTGGGGC CCATAATGTA 
TTTCATCCCC TGACCTATGA AGGAGGTGTA GACTTGAACA GCATCCAGGA TCCTGATGAG 
AAGGTAGCCA TGCTTACGCA AATCTTGGAA TTTGGGCAGA CACCAAAACA ACTATTTGTG 
ACACCACATC CTCGAAGGAT CACCCCAAAG TTTAAAAGTT TGTCCCAGAC CTCCAGTTAT 
AATGCTTCTA TGGCAGATTC CCCAGGTGAA GAGTCTTTTG AAGACCTGAC CGAAGAAAGC 
AAAACACTGG CCTGGAATAA CATCACCAAA CTGCAGTTAC ACGAGCACTA TAAAATCCAC 
AAAGAAGCAG TTACTGGAAT CACGGTCTCT CGCAATGGAT CTTCAGTATT CACAACATCC 
CAAGATTCCA CCTTGAAGAT GTTTTCTAAA GAATCAAAAA TGCTACAAAG AAGTATATCA 
TTTTCAAATA TGGCTTTATC GTCTTGTTTA CTTTTACCAG GAGATGCCAC TGTCATAACT 
TCTTCATGGG ATAATAATGT CTATTTTTAT TCCATAGCAT TTGGAAGACG CCAGGACACG 
TTAATGGGAC ATGATGATGC TGTTAGTAAG ATCTGTTGGC ATGACAACAG GCTATATTCT 
GCATCGTGGG ACTCTACAGT GAAGGTGTGG TCTGGTGTTC CTGCAGAGAT GCCAGGCACC 
AAAAGACACC ACTTTGACTT GCTGGCCGAG CTGGAACATG ATGTCAGTGT AGATACAATC 
AGTTTAAATG CTGCAAGCAC ACTGTTAGTT TCCGGCACCA AAGAAGGCAC AGTGAATATT 
TGGGACCTCA CAACGGCCAC CTTAATGCAC CAGATTCCAT GCCATTCAGG GATTGTATGT 
GACACTGCTT TTAGCCCAGA TAGTCGCCAT GTCCTCAGCA CAGGAACAGA TGGCTGTCTT 
AATGTCATTG ATGTGCAGAC AGGAATGCTC ATCTCCTCCA TGACATCAGA TGAGCCCCAG 
AGGTGCTTTG TCTGGGATGG AAATTCCGTT TTATCTGGCA GTCAGTCTGG TGAACTGCTC 
GTTTGGGACC TCCTTGGAGC AAAAATCAGT GAGAGAATAC AGGGCCACAC AGGTGCTGTG 
ACATGTATAT GGATGAATGA ACAGTGTAGC AGTATCATCA CAGGAGGGGA AGACAGACAA 
ATTATATTCT GGAAATTGCA GTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138244.1
CDS	62..2908
Translation	MTGQARFHRGAEACRAIRQCRSRRPRAAHPEGRAGSGRGSRHASPGVRRGGFSLLLLNLEEYYFEQHRANHILHKGSHHERKIRGSLKICSKSVIFEPDSISQPIIKIPLRDCIKIGKHGENGANRHFTKAKSGGISLIFSQVYFIKEHNVVAPYKIERGKMEYVFELDVPGKVEDVVETLLQLHRASCLDKLGDQTAMITAILQSRLARTSFDKNRFQNISEKLHMECKAEMVTPLVTNPGHVCITDTNLYFQPLNGYPKPVVQITLQDVRRIYKRRHGLMPLGLEVFCTEDDLCSDIYLKFYEPQDRDDLYFYIATYLEHHVAEHTAESYMLQWQRGHLSNYQYLLHLNNLADRSCNDLSQYPVFPWIIHDYSSSELDLSNPGTFRDLSKPVGALNKERLERLLTRYQEMPEPKFMYGSHYSSPGYVLFYLVRIAPEYMLCLQNGRFDNADRMFNSIAETWKNCLDGATDFKELIPEFYGDDVSFLVNSLKLDLGKRQGGQMVDDVELPPWASSPEDFLQKSKDALESNYVSEHLHEWIDLIFGYKQKGSDAVGAHNVFHPLTYEGGVDLNSIQDPDEKVAMLTQILEFGQTPKQLFVTPHPRRITPKFKSLSQTSSYNASMADSPGEESFEDLTEESKTLAWNNITKLQLHEHYKIHKEAVTGITVSRNGSSVFTTSQDSTLKMFSKESKMLQRSISFSNMALSSCLLLPGDATVITSSWDNNVYFYSIAFGRRQDTLMGHDDAVSKICWHDNRLYSASWDSTVKVWSGVPAEMPGTKRHHFDLLAELEHDVSVDTISLNAASTLLVSGTKEGTVNIWDLTTATLMHQIPCHSGIVCDTAFSPDSRHVLSTGTDGCLNVIDVQTGMLISSMTSDEPQRCFVWDGNSVLSGSQSGELLVWDLLGAKISERIQGHTGAVTCIWMNEQCSSIITGGEDRQIIFWKLQY

Target ORF information:

RefSeq Version	NM_001144772.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neutral sphingomyelinase activation associated factor (NSMAF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144772.1

ORF Insert Sequence:

ATGACGGGCC AGGCCAGATT CCACCGCGGA GCCGAGGCAT GCCGAGCTAT CCGGCAGTGC 
AGGTCCCGGC GGCCGCGGGC AGCTCACCCG GAAGGCCGCG CGGGGTCCGG GCGTGGATCC 
CGGCACGCGA GCCCGGGCGT TCGCAGGGGA GGATTTTCCT TGCTGCTGCT TAACTTGGAG 
GAGTACTACT TTGAACAGCA TAGAGCCAAT CACATTTTGC ACAAGGGCAG TCACCATGAA 
AGGAAAATCA GAGGCTCCTT AAAAATATGT TCAAAATCGG TGATTTTTGA ACCAGATTCA 
ATATCCCAGC CCATCATCAA GATTCCTTTG AGAGACTGTA TAAAAATAGG AAAGCATGGA 
GAAAATGGAG CCAATAGACA CTTCACAAAG GCAAAATCTG GGGGTATTTC ACTCATTTTC 
AGTCAGGTAT ATTTCATTAA AGAACATAAT GTTGTTGCAC CATATAAAAT AGAAAGGGGC 
AAAATGGAAT ATGTTTTTGA ATTGGATGTT CCCGGGAAAG TGGAAGATGT TGTGGAGACG 
TTGCTTCAGC TTCACAGAGC ATCCTGCCTT GACAAATTGG GTGACCAAAC CGCCATGATA 
ACAGCTATTT TGCAGTCTCG TTTAGCTAGA ACATCATTTG ACAAAAACAG GTTCCAAAAC 
ATTTCTGAAA AGCTGCACAT GGAATGCAAA GCAGAAATGG TGACGCCTCT GGTGACTAAT 
CCTGGACACG TGTGCATCAC GGACACAAAC CTGTATTTTC AGCCCCTCAA CGGCTACCCG 
AAACCTGTGG TCCAGATAAC ACTCCAAGAT GTCCGCCGCA TCTACAAAAG GAGGCACGGC 
CTCATGCCTC TGGGCTTGGA AGTATTTTGC ACAGAAGATG ATCTGTGTTC CGACATCTAC 
CTAAAGTTCT ATGAACCTCA AGATAGAGAT GATCTCTATT TTTACATTGC CACATACCTA 
GAGCACCATG TGGCGGAGCA CACTGCTGAG AGCTACATGC TGCAGTGGCA GCGTGGACAC 
CTTTCCAACT ATCAGTACCT CCTTCACCTC AACAACCTGG CCGACCGCAG CTGCAACGAC 
CTCTCCCAGT ACCCTGTGTT TCCATGGATA ATACATGATT ATTCCAGCTC AGAACTAGAT 
TTGTCAAATC CAGGAACCTT CCGGGATCTC AGTAAGCCAG TAGGGGCCCT AAATAAGGAA 
CGGCTGGAGA GACTACTGAC ACGCTACCAG GAAATGCCTG AACCAAAGTT CATGTATGGG 
AGTCACTACT CTTCCCCGGG TTATGTACTT TTTTATCTTG TTAGGATTGC ACCAGAGTAT 
ATGCTGTGCC TGCAGAATGG AAGATTTGAT AATGCAGATA GAATGTTCAA CAGTATTGCA 
GAAACTTGGA AAAACTGTCT GGATGGTGCA ACGGATTTTA AAGAGTTAAT TCCAGAATTC 
TATGGTGATG ATGTGAGCTT TCTAGTCAAT AGCCTGAAGT TGGATTTGGG AAAGAGACAA 
GGAGGACAGA TGGTTGACGA CGTGGAGCTT CCCCCTTGGG CTTCCAGTCC CGAGGACTTT 
CTCCAGAAGA GCAAAGATGC ATTGGAAAGC AATTATGTGT CTGAACACCT TCACGAGTGG 
ATTGATCTAA TATTTGGCTA CAAACAAAAA GGGAGTGATG CAGTTGGGGC CCATAATGTA 
TTTCATCCCC TGACCTATGA AGGAGGTGTA GACTTGAACA GCATCCAGGA TCCTGATGAG 
AAGGTAGCCA TGCTTACGCA AATCTTGGAA TTTGGGCAGA CACCAAAACA ACTATTTGTG 
ACACCACATC CTCGAAGGAT CACCCCAAAG TTTAAAAGTT TGTCCCAGAC CTCCAGTTAT 
AATGCTTCTA TGGCAGATTC CCCAGGTGAA GAGTCTTTTG AAGACCTGAC CGAAGAAAGC 
AAAACACTGG CCTGGAATAA CATCACCAAA CTGCAGTTAC ACGAGCACTA TAAAATCCAC 
AAAGAAGCAG TTACTGGAAT CACGGTCTCT CGCAATGGAT CTTCAGTATT CACAACATCC 
CAAGATTCCA CCTTGAAGAT GTTTTCTAAA GAATCAAAAA TGCTACAAAG AAGTATATCA 
TTTTCAAATA TGGCTTTATC GTCTTGTTTA CTTTTACCAG GAGATGCCAC TGTCATAACT 
TCTTCATGGG ATAATAATGT CTATTTTTAT TCCATAGCAT TTGGAAGACG CCAGGACACG 
TTAATGGGAC ATGATGATGC TGTTAGTAAG ATCTGTTGGC ATGACAACAG GCTATATTCT 
GCATCGTGGG ACTCTACAGT GAAGGTGTGG TCTGGTGTTC CTGCAGAGAT GCCAGGCACC 
AAAAGACACC ACTTTGACTT GCTGGCCGAG CTGGAACATG ATGTCAGTGT AGATACAATC 
AGTTTAAATG CTGCAAGCAC ACTGTTAGTT TCCGGCACCA AAGAAGGCAC AGTGAATATT 
TGGGACCTCA CAACGGCCAC CTTAATGCAC CAGATTCCAT GCCATTCAGG GATTGTATGT 
GACACTGCTT TTAGCCCAGA TAGTCGCCAT GTCCTCAGCA CAGGAACAGA TGGCTGTCTT 
AATGTCATTG ATGTGCAGAC AGGAATGCTC ATCTCCTCCA TGACATCAGA TGAGCCCCAG 
AGGTGCTTTG TCTGGGATGG AAATTCCGTT TTATCTGGCA GTCAGTCTGG TGAACTGCTC 
GTTTGGGACC TCCTTGGAGC AAAAATCAGT GAGAGAATAC AGGGCCACAC AGGTGCTGTG 
ACATGTATAT GGATGAATGA ACAGTGTAGC AGTATCATCA CAGGAGGGGA AGACAGACAA 
ATTATATTCT GGAAATTGCA GTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Caspase-2 associates with FAN through direct interaction and overlapping functionality.
Biochemical and biophysical research communications499(4)822-828(2018 05)
Forsberg J,Li X,Zamaraev AV,Panaretakis T,Zhivotovsky B,Olsson M

New variant identified in major susceptibility locus to tuberculosis on chromosomal region 8q12-q13 in Moroccan population: a case control study.
BMC infectious diseases17(1)712(2017 11)
Qrafli M,Asekkaj I,Bourkadi JE,El Aouad R,Sadki K

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

The BEACH Domain Protein SPIRRIG Is Essential for Arabidopsis Salt Stress Tolerance and Functions as a Regulator of Transcript Stabilization and Localization.
PLoS biology13(7)e1002188(2015 Jul)
Steffens A,Bräutigam A,Jakoby M,Hülskamp M

Loci associated with N-glycosylation of human immunoglobulin G show pleiotropy with autoimmune diseases and haematological cancers.
PLoS genetics9(1)e1003225(2013)
Lauc G,Huffman JE,Pučić M,Zgaga L,Adamczyk B,Mužinić A,Novokmet M,Polašek O,Gornik O,Krištić J,Keser T,Vitart V,Scheijen B,Uh HW,Molokhia M,Patrick AL,McKeigue P,Kolčić I,Lukić IK,Swann O,van Leeuwen FN,Ruhaak LR,Houwing-Duistermaat JJ,Slagboom PE,Beekman M,de Craen AJ,Deelder AM,Zeng Q,Wang W,Hastie ND,Gyllensten U,Wilson JF,Wuhrer M,Wright AF,Rudd PM,Hayward C,Aulchenko Y,Campbell H,Rudan I

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NSMAF cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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