AKT1S1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AKT1S1
Entrez Gene ID	84335
Full Name	AKT1 substrate 1
Synonyms	Lobe,PRAS40
General protein information	Preferred Names AKT1 substrate 1 Names proline-rich AKT1 substrate 1 40 kDa proline-rich AKT substrate AKT1 substrate 1 (proline rich)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.33
Summary	AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008].
Disorder MIM:	610221

mRNA and Protein(s)

mRNA	Protein	Name
NM_001098633.3	NP_001092103.1	proline-rich AKT1 substrate 1 isoform b
NM_001098633.4	NP_001092103.1	proline-rich AKT1 substrate 1 isoform b
NM_032375.5	NP_115751.3	proline-rich AKT1 substrate 1 isoform a
NM_001278160.1	NP_001265089.1	proline-rich AKT1 substrate 1 isoform b
NM_001278159.1	NP_001265088.1	proline-rich AKT1 substrate 1 isoform b
NM_001098632.2	NP_001092102.1	proline-rich AKT1 substrate 1 isoform b

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	AKT1S1	NP_001233316.1
Rattus norvegicus (Norway rat)	Akt1s1	NP_001099729.2
Homo sapiens (human)	AKT1S1	NP_115751.3
Macaca mulatta (Rhesus monkey)	LOC719101	XP_001115723.2
Canis lupus familiaris (dog)	AKT1S1	XP_005616340.1
Bos taurus (cattle)	AKT1S1	NP_001076903.1
Mus musculus (house mouse)	Akt1s1	NP_080546.1
Xenopus tropicalis (tropical clawed frog)	akt1s1	NP_001011102.1

Gene Ontology
Bibliography

Related articles in PubMed

Bloom Syndrome Protein Activates AKT and PRAS40 in Prostate Cancer Cells.
Chen K, Xu H, Zhao J
Oxidative medicine and cellular longevity20193685817(2019)

Mechanisms of mTORC1 activation by RHEB and inhibition by PRAS40.
Yang H, Jiang X, Li B, Yang HJ, Miller M, Yang A, Dhar A, Pavletich NP
Nature552(7685)368-373(2017 12)

PRAS40 Connects Microenvironmental Stress Signaling to Exosome-Mediated Secretion.
Guo J, Jayaprakash P, Dan J, Wise P, Jang GB, Liang C, Chen M, Woodley DT, Fabbri M, Li W
Molecular and cellular biology37(19)(2017 Oct)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Uric acid priming in human monocytes is driven by the AKT-PRAS40 autophagy pathway.
Crişan TO, Cleophas MCP, Novakovic B, Erler K, van de Veerdonk FL, Stunnenberg HG, Netea MG, Dinarello CA, Joosten LAB
Proceedings of the National Academy of Sciences of the United States of America114(21)5485-5490(2017 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AKT1S1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AKT1S1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu08534	NM_032375.5 Latest version!	Homo sapiens AKT1 substrate 1 (AKT1S1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu05653	NM_001278159.1 Latest version!	Homo sapiens AKT1 substrate 1 (AKT1S1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu05653	NM_001098632.2 Latest version!	Homo sapiens AKT1 substrate 1 (AKT1S1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu05653	NM_001098633.4 Latest version!	Homo sapiens AKT1 substrate 1 (AKT1S1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu05653	NM_001278160.1 Latest version!	Homo sapiens AKT1 substrate 1 (AKT1S1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
View Old Accession Versions >>
OHu05653	NM_001098633.3	Homo sapiens AKT1 substrate 1 (AKT1S1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AKT1S1 ( NM_032375.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_115751.3 Homo sapiens AKT1 substrate 1 (AKT1S1), transcript variant 1, mRNA.

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CloneID	OHu08534
Clone ID Related Accession (Same CDS sequence)	NM_032375.5
Accession Version	NM_032375.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Homo sapiens(human)
Product	proline-rich AKT1 substrate 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA005259.1, AK123687.1 and AC118341.2. On May 14, 2013 this sequence version replaced NM_032375.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK123687.1, SRR1163655.119928.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTTTTG AGGGCGGTGA CGGCGCCGGG CCGGCCATGC TGGCTACGGG CACGGCGCGG 
ATGGCGTCGG GGCGCCCCGA GGAGCTGTGG GAGGCCGTGG TGGGGGCCGC TGAGCGCTTC 
CGGGCCCGGA CTGGCACGGA GCTGGTGCTG CTGACCGCGG CCCCGCCGCC ACCACCCCGC 
CCGGGCCCCT GTGCCTATGC TGCCCATGGT CGAGGAGCCC TGGCGGAGGC AGCGCGCCGT 
TGCCTCCACG ACATCGCACT GGCCCACAGG GCTGCCACTG CTGCTCGGCC TCCTGCGCCC 
CCACCAGCAC CACAGCCACC CAGTCCCACA CCCAGCCCAC CCCGGCCTAC CCTGGCCAGA 
GAGGACAACG AGGAGGACGA GGATGAGCCC ACAGAGACAG AGACCTCCGG GGAGCAGCTG 
GGCATTAGTG ATAATGGAGG GCTCTTTGTG ATGGATGAGG ACGCCACCCT CCAGGACCTT 
CCCCCCTTCT GTGAGTCAGA CCCCGAGAGT ACAGATGATG GCAGCCTGAG CGAGGAGACC 
CCCGCCGGCC CCCCCACCTG CTCAGTGCCC CCAGCCTCAG CCCTACCCAC ACAGCAGTAC 
GCCAAGTCCC TGCCTGTGTC TGTGCCCGTC TGGGGCTTCA AGGAGAAGAG GACAGAGGCG 
CGGTCATCAG ATGAGGAGAA TGGGCCGCCC TCTTCGCCCG ACCTGGACCG CATCGCGGCG 
AGCATGCGCG CGCTGGTGCT GCGAGAGGCC GAGGACACCC AGGTCTTCGG GGACCTGCCA 
CGGCCGCGGC TTAACACCAG CGACTTCCAG AAGCTGAAGC GGAAATATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115751.3
CDS	733..1563
Translation	MSFEGGDGAGPAMLATGTARMASGRPEELWEAVVGAAERFRARTGTELVLLTAAPPPPPRPGPCAYAAHGRGALAEAARRCLHDIALAHRAATAARPPAPPPAPQPPSPTPSPPRPTLAREDNEEDEDEPTETETSGEQLGISDNGGLFVMDEDATLQDLPPFCESDPESTDDGSLSEETPAGPPTCSVPPASALPTQQYAKSLPVSVPVWGFKEKRTEARSSDEENGPPSSPDLDRIAASMRALVLREAEDTQVFGDLPRPRLNTSDFQKLKRKY

Target ORF information:

RefSeq Version	NM_032375.5
Organism	Homo sapiens(human)
Definition	Homo sapiens AKT1 substrate 1 (AKT1S1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032375.5

ORF Insert Sequence:

ATGTCTTTTG AGGGCGGTGA CGGCGCCGGG CCGGCCATGC TGGCTACGGG CACGGCGCGG 
ATGGCGTCGG GGCGCCCCGA GGAGCTGTGG GAGGCCGTGG TGGGGGCCGC TGAGCGCTTC 
CGGGCCCGGA CTGGCACGGA GCTGGTGCTG CTGACCGCGG CCCCGCCGCC ACCACCCCGC 
CCGGGCCCCT GTGCCTATGC TGCCCATGGT CGAGGAGCCC TGGCGGAGGC AGCGCGCCGT 
TGCCTCCACG ACATCGCACT GGCCCACAGG GCTGCCACTG CTGCTCGGCC TCCTGCGCCC 
CCACCAGCAC CACAGCCACC CAGTCCCACA CCCAGCCCAC CCCGGCCTAC CCTGGCCAGA 
GAGGACAACG AGGAGGACGA GGATGAGCCC ACAGAGACAG AGACCTCCGG GGAGCAGCTG 
GGCATTAGTG ATAATGGAGG GCTCTTTGTG ATGGATGAGG ACGCCACCCT CCAGGACCTT 
CCCCCCTTCT GTGAGTCAGA CCCCGAGAGT ACAGATGATG GCAGCCTGAG CGAGGAGACC 
CCCGCCGGCC CCCCCACCTG CTCAGTGCCC CCAGCCTCAG CCCTACCCAC ACAGCAGTAC 
GCCAAGTCCC TGCCTGTGTC TGTGCCCGTC TGGGGCTTCA AGGAGAAGAG GACAGAGGCG 
CGGTCATCAG ATGAGGAGAA TGGGCCGCCC TCTTCGCCCG ACCTGGACCG CATCGCGGCG 
AGCATGCGCG CGCTGGTGCT GCGAGAGGCC GAGGACACCC AGGTCTTCGG GGACCTGCCA 
CGGCCGCGGC TTAACACCAG CGACTTCCAG AAGCTGAAGC GGAAATATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKT1S1 ( NM_001098633.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001092103.1 Homo sapiens AKT1 substrate 1 (AKT1S1), transcript variant 3, mRNA.

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CloneID	OHu05653
Clone ID Related Accession (Same CDS sequence)	NM_001098633.3 , NM_001098633.4 , NM_001278160.1 , NM_001278159.1 , NM_001098632.2
Accession Version	NM_001098633.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550851200000
Organism	Homo sapiens(human)
Product	proline-rich AKT1 substrate 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA848626.1, BC051844.2 and AC118341.2. On May 14, 2013 this sequence version replaced NM_001098633.2. Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, 4, and 5 encode the same isoform (b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.242210.1, AL523463.3 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCGG GGCGCCCCGA GGAGCTGTGG GAGGCCGTGG TGGGGGCCGC TGAGCGCTTC 
CGGGCCCGGA CTGGCACGGA GCTGGTGCTG CTGACCGCGG CCCCGCCGCC ACCACCCCGC 
CCGGGCCCCT GTGCCTATGC TGCCCATGGT CGAGGAGCCC TGGCGGAGGC AGCGCGCCGT 
TGCCTCCACG ACATCGCACT GGCCCACAGG GCTGCCACTG CTGCTCGGCC TCCTGCGCCC 
CCACCAGCAC CACAGCCACC CAGTCCCACA CCCAGCCCAC CCCGGCCTAC CCTGGCCAGA 
GAGGACAACG AGGAGGACGA GGATGAGCCC ACAGAGACAG AGACCTCCGG GGAGCAGCTG 
GGCATTAGTG ATAATGGAGG GCTCTTTGTG ATGGATGAGG ACGCCACCCT CCAGGACCTT 
CCCCCCTTCT GTGAGTCAGA CCCCGAGAGT ACAGATGATG GCAGCCTGAG CGAGGAGACC 
CCCGCCGGCC CCCCCACCTG CTCAGTGCCC CCAGCCTCAG CCCTACCCAC ACAGCAGTAC 
GCCAAGTCCC TGCCTGTGTC TGTGCCCGTC TGGGGCTTCA AGGAGAAGAG GACAGAGGCG 
CGGTCATCAG ATGAGGAGAA TGGGCCGCCC TCTTCGCCCG ACCTGGACCG CATCGCGGCG 
AGCATGCGCG CGCTGGTGCT GCGAGAGGCC GAGGACACCC AGGTCTTCGG GGACCTGCCA 
CGGCCGCGGC TTAACACCAG CGACTTCCAG AAGCTGAAGC GGAAATATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092103.1
CDS	159..929
Translation	MASGRPEELWEAVVGAAERFRARTGTELVLLTAAPPPPPRPGPCAYAAHGRGALAEAARRCLHDIALAHRAATAARPPAPPPAPQPPSPTPSPPRPTLAREDNEEDEDEPTETETSGEQLGISDNGGLFVMDEDATLQDLPPFCESDPESTDDGSLSEETPAGPPTCSVPPASALPTQQYAKSLPVSVPVWGFKEKRTEARSSDEENGPPSSPDLDRIAASMRALVLREAEDTQVFGDLPRPRLNTSDFQKLKRKY

Target ORF information:

RefSeq Version	NM_001098633.3
Organism	Homo sapiens(human)
Definition	Homo sapiens AKT1 substrate 1 (AKT1S1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098633.3

ORF Insert Sequence:

ATGGCGTCGG GGCGCCCCGA GGAGCTGTGG GAGGCCGTGG TGGGGGCCGC TGAGCGCTTC 
CGGGCCCGGA CTGGCACGGA GCTGGTGCTG CTGACCGCGG CCCCGCCGCC ACCACCCCGC 
CCGGGCCCCT GTGCCTATGC TGCCCATGGT CGAGGAGCCC TGGCGGAGGC AGCGCGCCGT 
TGCCTCCACG ACATCGCACT GGCCCACAGG GCTGCCACTG CTGCTCGGCC TCCTGCGCCC 
CCACCAGCAC CACAGCCACC CAGTCCCACA CCCAGCCCAC CCCGGCCTAC CCTGGCCAGA 
GAGGACAACG AGGAGGACGA GGATGAGCCC ACAGAGACAG AGACCTCCGG GGAGCAGCTG 
GGCATTAGTG ATAATGGAGG GCTCTTTGTG ATGGATGAGG ACGCCACCCT CCAGGACCTT 
CCCCCCTTCT GTGAGTCAGA CCCCGAGAGT ACAGATGATG GCAGCCTGAG CGAGGAGACC 
CCCGCCGGCC CCCCCACCTG CTCAGTGCCC CCAGCCTCAG CCCTACCCAC ACAGCAGTAC 
GCCAAGTCCC TGCCTGTGTC TGTGCCCGTC TGGGGCTTCA AGGAGAAGAG GACAGAGGCG 
CGGTCATCAG ATGAGGAGAA TGGGCCGCCC TCTTCGCCCG ACCTGGACCG CATCGCGGCG 
AGCATGCGCG CGCTGGTGCT GCGAGAGGCC GAGGACACCC AGGTCTTCGG GGACCTGCCA 
CGGCCGCGGC TTAACACCAG CGACTTCCAG AAGCTGAAGC GGAAATATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKT1S1 ( NM_001098633.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001092103.1 Homo sapiens AKT1 substrate 1 (AKT1S1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu05653
Clone ID Related Accession (Same CDS sequence)	NM_001098633.3 , NM_001098633.4 , NM_001278160.1 , NM_001278159.1 , NM_001098632.2
Accession Version	NM_001098633.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577980800000
Organism	Homo sapiens(human)
Product	proline-rich AKT1 substrate 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA848626.1 and BC051844.2. On Jun 2, 2019 this sequence version replaced NM_001098633.3. Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, 4, and 5 encode the same isoform (b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.242210.1, SRR3476690.552740.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCGG GGCGCCCCGA GGAGCTGTGG GAGGCCGTGG TGGGGGCCGC TGAGCGCTTC 
CGGGCCCGGA CTGGCACGGA GCTGGTGCTG CTGACCGCGG CCCCGCCGCC ACCACCCCGC 
CCGGGCCCCT GTGCCTATGC TGCCCATGGT CGAGGAGCCC TGGCGGAGGC AGCGCGCCGT 
TGCCTCCACG ACATCGCACT GGCCCACAGG GCTGCCACTG CTGCTCGGCC TCCTGCGCCC 
CCACCAGCAC CACAGCCACC CAGTCCCACA CCCAGCCCAC CCCGGCCTAC CCTGGCCAGA 
GAGGACAACG AGGAGGACGA GGATGAGCCC ACAGAGACAG AGACCTCCGG GGAGCAGCTG 
GGCATTAGTG ATAATGGAGG GCTCTTTGTG ATGGATGAGG ACGCCACCCT CCAGGACCTT 
CCCCCCTTCT GTGAGTCAGA CCCCGAGAGT ACAGATGATG GCAGCCTGAG CGAGGAGACC 
CCCGCCGGCC CCCCCACCTG CTCAGTGCCC CCAGCCTCAG CCCTACCCAC ACAGCAGTAC 
GCCAAGTCCC TGCCTGTGTC TGTGCCCGTC TGGGGCTTCA AGGAGAAGAG GACAGAGGCG 
CGGTCATCAG ATGAGGAGAA TGGGCCGCCC TCTTCGCCCG ACCTGGACCG CATCGCGGCG 
AGCATGCGCG CGCTGGTGCT GCGAGAGGCC GAGGACACCC AGGTCTTCGG GGACCTGCCA 
CGGCCGCGGC TTAACACCAG CGACTTCCAG AAGCTGAAGC GGAAATATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092103.1
CDS	127..897
Translation	MASGRPEELWEAVVGAAERFRARTGTELVLLTAAPPPPPRPGPCAYAAHGRGALAEAARRCLHDIALAHRAATAARPPAPPPAPQPPSPTPSPPRPTLAREDNEEDEDEPTETETSGEQLGISDNGGLFVMDEDATLQDLPPFCESDPESTDDGSLSEETPAGPPTCSVPPASALPTQQYAKSLPVSVPVWGFKEKRTEARSSDEENGPPSSPDLDRIAASMRALVLREAEDTQVFGDLPRPRLNTSDFQKLKRKY

Target ORF information:

RefSeq Version	NM_001098633.4
Organism	Homo sapiens(human)
Definition	Homo sapiens AKT1 substrate 1 (AKT1S1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098633.4

ORF Insert Sequence:

ATGGCGTCGG GGCGCCCCGA GGAGCTGTGG GAGGCCGTGG TGGGGGCCGC TGAGCGCTTC 
CGGGCCCGGA CTGGCACGGA GCTGGTGCTG CTGACCGCGG CCCCGCCGCC ACCACCCCGC 
CCGGGCCCCT GTGCCTATGC TGCCCATGGT CGAGGAGCCC TGGCGGAGGC AGCGCGCCGT 
TGCCTCCACG ACATCGCACT GGCCCACAGG GCTGCCACTG CTGCTCGGCC TCCTGCGCCC 
CCACCAGCAC CACAGCCACC CAGTCCCACA CCCAGCCCAC CCCGGCCTAC CCTGGCCAGA 
GAGGACAACG AGGAGGACGA GGATGAGCCC ACAGAGACAG AGACCTCCGG GGAGCAGCTG 
GGCATTAGTG ATAATGGAGG GCTCTTTGTG ATGGATGAGG ACGCCACCCT CCAGGACCTT 
CCCCCCTTCT GTGAGTCAGA CCCCGAGAGT ACAGATGATG GCAGCCTGAG CGAGGAGACC 
CCCGCCGGCC CCCCCACCTG CTCAGTGCCC CCAGCCTCAG CCCTACCCAC ACAGCAGTAC 
GCCAAGTCCC TGCCTGTGTC TGTGCCCGTC TGGGGCTTCA AGGAGAAGAG GACAGAGGCG 
CGGTCATCAG ATGAGGAGAA TGGGCCGCCC TCTTCGCCCG ACCTGGACCG CATCGCGGCG 
AGCATGCGCG CGCTGGTGCT GCGAGAGGCC GAGGACACCC AGGTCTTCGG GGACCTGCCA 
CGGCCGCGGC TTAACACCAG CGACTTCCAG AAGCTGAAGC GGAAATATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKT1S1 ( NM_001278160.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265089.1 Homo sapiens AKT1 substrate 1 (AKT1S1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu05653
Clone ID Related Accession (Same CDS sequence)	NM_001098633.3 , NM_001098633.4 , NM_001278160.1 , NM_001278159.1 , NM_001098632.2
Accession Version	NM_001278160.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Homo sapiens(human)
Product	proline-rich AKT1 substrate 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA476468.1, BC051844.2 and AC118341.2. Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, 4, and 5 encode the same isoform (b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC007416.2, BC022241.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCGG GGCGCCCCGA GGAGCTGTGG GAGGCCGTGG TGGGGGCCGC TGAGCGCTTC 
CGGGCCCGGA CTGGCACGGA GCTGGTGCTG CTGACCGCGG CCCCGCCGCC ACCACCCCGC 
CCGGGCCCCT GTGCCTATGC TGCCCATGGT CGAGGAGCCC TGGCGGAGGC AGCGCGCCGT 
TGCCTCCACG ACATCGCACT GGCCCACAGG GCTGCCACTG CTGCTCGGCC TCCTGCGCCC 
CCACCAGCAC CACAGCCACC CAGTCCCACA CCCAGCCCAC CCCGGCCTAC CCTGGCCAGA 
GAGGACAACG AGGAGGACGA GGATGAGCCC ACAGAGACAG AGACCTCCGG GGAGCAGCTG 
GGCATTAGTG ATAATGGAGG GCTCTTTGTG ATGGATGAGG ACGCCACCCT CCAGGACCTT 
CCCCCCTTCT GTGAGTCAGA CCCCGAGAGT ACAGATGATG GCAGCCTGAG CGAGGAGACC 
CCCGCCGGCC CCCCCACCTG CTCAGTGCCC CCAGCCTCAG CCCTACCCAC ACAGCAGTAC 
GCCAAGTCCC TGCCTGTGTC TGTGCCCGTC TGGGGCTTCA AGGAGAAGAG GACAGAGGCG 
CGGTCATCAG ATGAGGAGAA TGGGCCGCCC TCTTCGCCCG ACCTGGACCG CATCGCGGCG 
AGCATGCGCG CGCTGGTGCT GCGAGAGGCC GAGGACACCC AGGTCTTCGG GGACCTGCCA 
CGGCCGCGGC TTAACACCAG CGACTTCCAG AAGCTGAAGC GGAAATATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265089.1
CDS	195..965
Translation	MASGRPEELWEAVVGAAERFRARTGTELVLLTAAPPPPPRPGPCAYAAHGRGALAEAARRCLHDIALAHRAATAARPPAPPPAPQPPSPTPSPPRPTLAREDNEEDEDEPTETETSGEQLGISDNGGLFVMDEDATLQDLPPFCESDPESTDDGSLSEETPAGPPTCSVPPASALPTQQYAKSLPVSVPVWGFKEKRTEARSSDEENGPPSSPDLDRIAASMRALVLREAEDTQVFGDLPRPRLNTSDFQKLKRKY

Target ORF information:

RefSeq Version	NM_001278160.1
Organism	Homo sapiens(human)
Definition	Homo sapiens AKT1 substrate 1 (AKT1S1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278160.1

ORF Insert Sequence:

ATGGCGTCGG GGCGCCCCGA GGAGCTGTGG GAGGCCGTGG TGGGGGCCGC TGAGCGCTTC 
CGGGCCCGGA CTGGCACGGA GCTGGTGCTG CTGACCGCGG CCCCGCCGCC ACCACCCCGC 
CCGGGCCCCT GTGCCTATGC TGCCCATGGT CGAGGAGCCC TGGCGGAGGC AGCGCGCCGT 
TGCCTCCACG ACATCGCACT GGCCCACAGG GCTGCCACTG CTGCTCGGCC TCCTGCGCCC 
CCACCAGCAC CACAGCCACC CAGTCCCACA CCCAGCCCAC CCCGGCCTAC CCTGGCCAGA 
GAGGACAACG AGGAGGACGA GGATGAGCCC ACAGAGACAG AGACCTCCGG GGAGCAGCTG 
GGCATTAGTG ATAATGGAGG GCTCTTTGTG ATGGATGAGG ACGCCACCCT CCAGGACCTT 
CCCCCCTTCT GTGAGTCAGA CCCCGAGAGT ACAGATGATG GCAGCCTGAG CGAGGAGACC 
CCCGCCGGCC CCCCCACCTG CTCAGTGCCC CCAGCCTCAG CCCTACCCAC ACAGCAGTAC 
GCCAAGTCCC TGCCTGTGTC TGTGCCCGTC TGGGGCTTCA AGGAGAAGAG GACAGAGGCG 
CGGTCATCAG ATGAGGAGAA TGGGCCGCCC TCTTCGCCCG ACCTGGACCG CATCGCGGCG 
AGCATGCGCG CGCTGGTGCT GCGAGAGGCC GAGGACACCC AGGTCTTCGG GGACCTGCCA 
CGGCCGCGGC TTAACACCAG CGACTTCCAG AAGCTGAAGC GGAAATATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKT1S1 ( NM_001278159.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265088.1 Homo sapiens AKT1 substrate 1 (AKT1S1), transcript variant 4, mRNA.

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CloneID	OHu05653
Clone ID Related Accession (Same CDS sequence)	NM_001098633.3 , NM_001098633.4 , NM_001278160.1 , NM_001278159.1 , NM_001098632.2
Accession Version	NM_001278159.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Homo sapiens(human)
Product	proline-rich AKT1 substrate 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK055511.1, BC051844.2 and AC118341.2. Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, 4, and 5 encode the same isoform (b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK055511.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCGG GGCGCCCCGA GGAGCTGTGG GAGGCCGTGG TGGGGGCCGC TGAGCGCTTC 
CGGGCCCGGA CTGGCACGGA GCTGGTGCTG CTGACCGCGG CCCCGCCGCC ACCACCCCGC 
CCGGGCCCCT GTGCCTATGC TGCCCATGGT CGAGGAGCCC TGGCGGAGGC AGCGCGCCGT 
TGCCTCCACG ACATCGCACT GGCCCACAGG GCTGCCACTG CTGCTCGGCC TCCTGCGCCC 
CCACCAGCAC CACAGCCACC CAGTCCCACA CCCAGCCCAC CCCGGCCTAC CCTGGCCAGA 
GAGGACAACG AGGAGGACGA GGATGAGCCC ACAGAGACAG AGACCTCCGG GGAGCAGCTG 
GGCATTAGTG ATAATGGAGG GCTCTTTGTG ATGGATGAGG ACGCCACCCT CCAGGACCTT 
CCCCCCTTCT GTGAGTCAGA CCCCGAGAGT ACAGATGATG GCAGCCTGAG CGAGGAGACC 
CCCGCCGGCC CCCCCACCTG CTCAGTGCCC CCAGCCTCAG CCCTACCCAC ACAGCAGTAC 
GCCAAGTCCC TGCCTGTGTC TGTGCCCGTC TGGGGCTTCA AGGAGAAGAG GACAGAGGCG 
CGGTCATCAG ATGAGGAGAA TGGGCCGCCC TCTTCGCCCG ACCTGGACCG CATCGCGGCG 
AGCATGCGCG CGCTGGTGCT GCGAGAGGCC GAGGACACCC AGGTCTTCGG GGACCTGCCA 
CGGCCGCGGC TTAACACCAG CGACTTCCAG AAGCTGAAGC GGAAATATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265088.1
CDS	384..1154
Translation	MASGRPEELWEAVVGAAERFRARTGTELVLLTAAPPPPPRPGPCAYAAHGRGALAEAARRCLHDIALAHRAATAARPPAPPPAPQPPSPTPSPPRPTLAREDNEEDEDEPTETETSGEQLGISDNGGLFVMDEDATLQDLPPFCESDPESTDDGSLSEETPAGPPTCSVPPASALPTQQYAKSLPVSVPVWGFKEKRTEARSSDEENGPPSSPDLDRIAASMRALVLREAEDTQVFGDLPRPRLNTSDFQKLKRKY

Target ORF information:

RefSeq Version	NM_001278159.1
Organism	Homo sapiens(human)
Definition	Homo sapiens AKT1 substrate 1 (AKT1S1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278159.1

ORF Insert Sequence:

ATGGCGTCGG GGCGCCCCGA GGAGCTGTGG GAGGCCGTGG TGGGGGCCGC TGAGCGCTTC 
CGGGCCCGGA CTGGCACGGA GCTGGTGCTG CTGACCGCGG CCCCGCCGCC ACCACCCCGC 
CCGGGCCCCT GTGCCTATGC TGCCCATGGT CGAGGAGCCC TGGCGGAGGC AGCGCGCCGT 
TGCCTCCACG ACATCGCACT GGCCCACAGG GCTGCCACTG CTGCTCGGCC TCCTGCGCCC 
CCACCAGCAC CACAGCCACC CAGTCCCACA CCCAGCCCAC CCCGGCCTAC CCTGGCCAGA 
GAGGACAACG AGGAGGACGA GGATGAGCCC ACAGAGACAG AGACCTCCGG GGAGCAGCTG 
GGCATTAGTG ATAATGGAGG GCTCTTTGTG ATGGATGAGG ACGCCACCCT CCAGGACCTT 
CCCCCCTTCT GTGAGTCAGA CCCCGAGAGT ACAGATGATG GCAGCCTGAG CGAGGAGACC 
CCCGCCGGCC CCCCCACCTG CTCAGTGCCC CCAGCCTCAG CCCTACCCAC ACAGCAGTAC 
GCCAAGTCCC TGCCTGTGTC TGTGCCCGTC TGGGGCTTCA AGGAGAAGAG GACAGAGGCG 
CGGTCATCAG ATGAGGAGAA TGGGCCGCCC TCTTCGCCCG ACCTGGACCG CATCGCGGCG 
AGCATGCGCG CGCTGGTGCT GCGAGAGGCC GAGGACACCC AGGTCTTCGG GGACCTGCCA 
CGGCCGCGGC TTAACACCAG CGACTTCCAG AAGCTGAAGC GGAAATATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKT1S1 ( NM_001098632.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001092102.1 Homo sapiens AKT1 substrate 1 (AKT1S1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu05653
Clone ID Related Accession (Same CDS sequence)	NM_001098633.3 , NM_001098633.4 , NM_001278160.1 , NM_001278159.1 , NM_001098632.2
Accession Version	NM_001098632.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Homo sapiens(human)
Product	proline-rich AKT1 substrate 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA005259.1, BC051844.2 and AC118341.2. On May 14, 2013 this sequence version replaced NM_001098632.1. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 2, 3, 4, and 5 encode the same isoform (b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC051844.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCGG GGCGCCCCGA GGAGCTGTGG GAGGCCGTGG TGGGGGCCGC TGAGCGCTTC 
CGGGCCCGGA CTGGCACGGA GCTGGTGCTG CTGACCGCGG CCCCGCCGCC ACCACCCCGC 
CCGGGCCCCT GTGCCTATGC TGCCCATGGT CGAGGAGCCC TGGCGGAGGC AGCGCGCCGT 
TGCCTCCACG ACATCGCACT GGCCCACAGG GCTGCCACTG CTGCTCGGCC TCCTGCGCCC 
CCACCAGCAC CACAGCCACC CAGTCCCACA CCCAGCCCAC CCCGGCCTAC CCTGGCCAGA 
GAGGACAACG AGGAGGACGA GGATGAGCCC ACAGAGACAG AGACCTCCGG GGAGCAGCTG 
GGCATTAGTG ATAATGGAGG GCTCTTTGTG ATGGATGAGG ACGCCACCCT CCAGGACCTT 
CCCCCCTTCT GTGAGTCAGA CCCCGAGAGT ACAGATGATG GCAGCCTGAG CGAGGAGACC 
CCCGCCGGCC CCCCCACCTG CTCAGTGCCC CCAGCCTCAG CCCTACCCAC ACAGCAGTAC 
GCCAAGTCCC TGCCTGTGTC TGTGCCCGTC TGGGGCTTCA AGGAGAAGAG GACAGAGGCG 
CGGTCATCAG ATGAGGAGAA TGGGCCGCCC TCTTCGCCCG ACCTGGACCG CATCGCGGCG 
AGCATGCGCG CGCTGGTGCT GCGAGAGGCC GAGGACACCC AGGTCTTCGG GGACCTGCCA 
CGGCCGCGGC TTAACACCAG CGACTTCCAG AAGCTGAAGC GGAAATATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092102.1
CDS	371..1141
Translation	MASGRPEELWEAVVGAAERFRARTGTELVLLTAAPPPPPRPGPCAYAAHGRGALAEAARRCLHDIALAHRAATAARPPAPPPAPQPPSPTPSPPRPTLAREDNEEDEDEPTETETSGEQLGISDNGGLFVMDEDATLQDLPPFCESDPESTDDGSLSEETPAGPPTCSVPPASALPTQQYAKSLPVSVPVWGFKEKRTEARSSDEENGPPSSPDLDRIAASMRALVLREAEDTQVFGDLPRPRLNTSDFQKLKRKY

Target ORF information:

RefSeq Version	NM_001098632.2
Organism	Homo sapiens(human)
Definition	Homo sapiens AKT1 substrate 1 (AKT1S1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098632.2

ORF Insert Sequence:

ATGGCGTCGG GGCGCCCCGA GGAGCTGTGG GAGGCCGTGG TGGGGGCCGC TGAGCGCTTC 
CGGGCCCGGA CTGGCACGGA GCTGGTGCTG CTGACCGCGG CCCCGCCGCC ACCACCCCGC 
CCGGGCCCCT GTGCCTATGC TGCCCATGGT CGAGGAGCCC TGGCGGAGGC AGCGCGCCGT 
TGCCTCCACG ACATCGCACT GGCCCACAGG GCTGCCACTG CTGCTCGGCC TCCTGCGCCC 
CCACCAGCAC CACAGCCACC CAGTCCCACA CCCAGCCCAC CCCGGCCTAC CCTGGCCAGA 
GAGGACAACG AGGAGGACGA GGATGAGCCC ACAGAGACAG AGACCTCCGG GGAGCAGCTG 
GGCATTAGTG ATAATGGAGG GCTCTTTGTG ATGGATGAGG ACGCCACCCT CCAGGACCTT 
CCCCCCTTCT GTGAGTCAGA CCCCGAGAGT ACAGATGATG GCAGCCTGAG CGAGGAGACC 
CCCGCCGGCC CCCCCACCTG CTCAGTGCCC CCAGCCTCAG CCCTACCCAC ACAGCAGTAC 
GCCAAGTCCC TGCCTGTGTC TGTGCCCGTC TGGGGCTTCA AGGAGAAGAG GACAGAGGCG 
CGGTCATCAG ATGAGGAGAA TGGGCCGCCC TCTTCGCCCG ACCTGGACCG CATCGCGGCG 
AGCATGCGCG CGCTGGTGCT GCGAGAGGCC GAGGACACCC AGGTCTTCGG GGACCTGCCA 
CGGCCGCGGC TTAACACCAG CGACTTCCAG AAGCTGAAGC GGAAATATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Bloom Syndrome Protein Activates AKT and PRAS40 in Prostate Cancer Cells.
Oxidative medicine and cellular longevity20193685817(2019)
Chen K,Xu H,Zhao J

Mechanisms of mTORC1 activation by RHEB and inhibition by PRAS40.
Nature552(7685)368-373(2017 12)
Yang H,Jiang X,Li B,Yang HJ,Miller M,Yang A,Dhar A,Pavletich NP

PRAS40 Connects Microenvironmental Stress Signaling to Exosome-Mediated Secretion.
Molecular and cellular biology37(19)(2017 Oct)
Guo J,Jayaprakash P,Dan J,Wise P,Jang GB,Liang C,Chen M,Woodley DT,Fabbri M,Li W

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Uric acid priming in human monocytes is driven by the AKT-PRAS40 autophagy pathway.
Proceedings of the National Academy of Sciences of the United States of America114(21)5485-5490(2017 05)
Crişan TO,Cleophas MCP,Novakovic B,Erler K,van de Veerdonk FL,Stunnenberg HG,Netea MG,Dinarello CA,Joosten LAB

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AKT1S1 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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