FZD8 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	FZD8
Entrez Gene ID	8325
Full Name	frizzled class receptor 8
Synonyms	FZ-8,hFZ8
General protein information	Preferred Names frizzled class receptor 8 Names frizzled-8 frizzled 8, seven transmembrane spanning receptor frizzled family receptor 8 frizzled homolog 8
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10p11.21
Summary	This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This gene is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined. [provided by RefSeq, Jul 2008].
Disorder MIM:	606146

mRNA and Protein(s)

mRNA	Protein	Name
NM_031866.2	NP_114072.1	frizzled-8 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	FZD8	NP_114072.1
Mus musculus (house mouse)	Fzd8	NP_032084.2
Rattus norvegicus (Norway rat)	Fzd8	NP_001037716.1
Rattus norvegicus (Norway rat)	LOC100909849	XP_003751769.1
Xenopus tropicalis (tropical clawed frog)	fzd8	NP_001090860.2
Canis lupus familiaris (dog)	FZD8	XP_005617125.1
Caenorhabditis elegans (roundworm)	cfz-2	NP_503965.2
Pan troglodytes (chimpanzee)	FZD8	XP_001142144.2
Danio rerio (zebrafish)	fzd8a	NP_570993.2
Macaca mulatta (Rhesus monkey)	FZD8	XP_001096837.1
Bos taurus (cattle)	FZD8	XP_005214377.1

Gene Ontology
Bibliography

Related articles in PubMed

Wnt receptor Frizzled 8 is a target of ERG in prostate cancer.
Chakravarthi BVSK, Chandrashekar DS, Hodigere Balasubramanya SA, Robinson AD, Carskadon S, Rao U, Gordetsky J, Manne U, Netto GJ, Sudarshan S, Palanisamy N, Varambally S
The Prostate78(16)1311-1320(2018 12)

Frizzled-8 integrates Wnt-11 and transforming growth factor-β signaling in prostate cancer.
Murillo-Garzón V, Gorroño-Etxebarria I, Åkerfelt M, Puustinen MC, Sistonen L, Nees M, Carton J, Waxman J, Kypta RM
Nature communications9(1)1747(2018 05)

MicroRNA-520b Suppresses Proliferation, Migration, and Invasion of Spinal Osteosarcoma Cells via Downregulation of Frizzled-8.
Wang J, Pang W, Zuo Z, Zhang W, He W
Oncology research25(8)1297-1304(2017 Sep)

FZD8, a target of p53, promotes bone metastasis in prostate cancer by activating canonical Wnt/β-catenin signaling.
Li Q, Ye L, Zhang X, Wang M, Lin C, Huang S, Guo W, Lai Y, Du H, Li J, Song L, Peng X
Cancer letters402166-176(2017 08)

Insights into Wnt binding and signalling from the structures of two Frizzled cysteine-rich domains.
Dann CE, Hsieh JC, Rattner A, Sharma D, Nathans J, Leahy DJ
Nature412(6842)86-90(2001 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following FZD8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FZD8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu24405	NM_031866.2 Latest version!	Homo sapiens frizzled class receptor 8 (FZD8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FZD8 ( NM_031866.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_114072.1 Homo sapiens frizzled class receptor 8 (FZD8), mRNA.

Price & Availability↑

CloneID	OHu24405
Clone ID Related Accession (Same CDS sequence)	NM_031866.2
Accession Version	NM_031866.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Homo sapiens(human)
Product	frizzled-8 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121749.14. This sequence is a reference standard in the RefSeqGene project. On Aug 27, 2008 this sequence version replaced NM_031866.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: AB043703.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGTGGG GTTACCTGTT GGAAGTGACC TCGCTGCTGG CCGCCTTGGC GCTGCTGCAG 
CGCTCTAGCG GCGCTGCGGC CGCCTCGGCC AAGGAGCTGG CATGCCAAGA GATCACCGTG 
CCGCTGTGTA AGGGCATCGG CTACAACTAC ACCTACATGC CCAATCAGTT CAACCACGAC 
ACGCAAGACG AGGCGGGCCT GGAGGTGCAC CAGTTCTGGC CGCTGGTGGA GATCCAGTGC 
TCGCCCGATC TCAAGTTCTT CCTGTGCAGC ATGTACACGC CCATCTGCCT AGAGGACTAC 
AAGAAGCCGC TGCCGCCCTG CCGCTCGGTG TGCGAGCGCG CCAAGGCCGG CTGCGCGCCG 
CTCATGCGCC AGTACGGCTT CGCCTGGCCC GACCGCATGC GCTGCGACCG GCTGCCCGAG 
CAAGGCAACC CTGACACGCT GTGCATGGAC TACAACCGCA CCGACCTAAC CACCGCCGCG 
CCCAGCCCGC CGCGCCGCCT GCCGCCGCCG CCGCCCGGCG AGCAGCCGCC TTCGGGCAGC 
GGCCACGGCC GCCCGCCGGG GGCCAGGCCC CCGCACCGCG GCGGCGGCAG GGGCGGTGGC 
GGCGGGGACG CGGCGGCGCC CCCAGCTCGC GGCGGCGGCG GTGGCGGGAA GGCGCGGCCC 
CCTGGCGGCG GCGCGGCTCC CTGCGAGCCC GGGTGCCAGT GCCGCGCGCC TATGGTGAGC 
GTGTCCAGCG AGCGCCACCC GCTCTACAAC CGCGTCAAGA CAGGCCAGAT CGCTAACTGC 
GCGCTGCCCT GCCACAACCC CTTTTTCAGC CAGGACGAGC GCGCCTTCAC CGTCTTCTGG 
ATCGGCCTGT GGTCGGTGCT CTGCTTCGTG TCCACCTTCG CCACCGTCTC CACCTTCCTT 
ATCGACATGG AGCGCTTCAA GTACCCGGAG CGGCCCATTA TCTTCCTCTC GGCCTGCTAC 
CTCTTCGTGT CGGTGGGCTA CCTAGTGCGC CTGGTGGCGG GCCACGAGAA GGTGGCGTGC 
AGCGGTGGCG CGCCGGGCGC GGGGGGCGCT GGGGGCGCGG GCGGCGCGGC GGCGGGCGCG 
GGCGCGGCGG GCGCGGGCGC GGGCGGCCCG GGCGGGCGCG GCGAGTACGA GGAGCTGGGC 
GCGGTGGAGC AGCACGTGCG CTACGAGACC ACCGGCCCCG CGCTGTGCAC CGTGGTCTTC 
TTGCTGGTCT ACTTCTTCGG CATGGCCAGC TCCATCTGGT GGGTGATCTT GTCGCTCACA 
TGGTTCCTGG CGGCCGGTAT GAAGTGGGGC AACGAAGCCA TCGCCGGCTA CTCGCAGTAC 
TTCCACCTGG CCGCGTGGCT TGTGCCCAGC GTCAAGTCCA TCGCGGTGCT GGCGCTCAGC 
TCGGTGGACG GCGACCCGGT GGCGGGCATC TGCTACGTGG GCAACCAGAG CCTGGACAAC 
CTGCGCGGCT TCGTGCTGGC GCCGCTGGTC ATCTACCTCT TCATCGGCAC CATGTTCCTG 
CTGGCCGGCT TCGTGTCCCT CTTCCGCATC CGCTCGGTCA TCAAGCAACA GGACGGCCCC 
ACCAAGACGC ACAAGCTGGA GAAGCTGATG ATCCGCCTGG GCCTGTTCAC CGTGCTCTAC 
ACCGTGCCCG CCGCGGTGGT GGTCGCCTGC CTCTTCTACG AGCAGCACAA CCGCCCGCGC 
TGGGAGGCCA CGCACAACTG CCCGTGCCTG CGGGACCTGC AGCCCGACCA GGCACGCAGG 
CCCGACTACG CCGTCTTCAT GCTCAAGTAC TTCATGTGCC TAGTGGTGGG CATCACCTCG 
GGCGTGTGGG TCTGGTCCGG CAAGACGCTG GAGTCCTGGC GCTCCCTGTG CACCCGCTGC 
TGCTGGGCCA GCAAGGGCGC CGCGGTGGGC GGGGGCGCGG GCGCCACGGC CGCGGGGGGT 
GGCGGCGGGC CGGGGGGCGG CGGCGGCGGG GGACCCGGCG GCGGCGGGGG GCCGGGCGGC 
GGCGGGGGCT CCCTCTACAG CGACGTCAGC ACTGGCCTGA CGTGGCGGTC GGGCACGGCG 
AGCTCCGTGT CTTATCCAAA GCAGATGCCA TTGTCCCAGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_114072.1
CDS	6..2090
Translation	MEWGYLLEVTSLLAALALLQRSSGAAAASAKELACQEITVPLCKGIGYNYTYMPNQFNHDTQDEAGLEVHQFWPLVEIQCSPDLKFFLCSMYTPICLEDYKKPLPPCRSVCERAKAGCAPLMRQYGFAWPDRMRCDRLPEQGNPDTLCMDYNRTDLTTAAPSPPRRLPPPPPGEQPPSGSGHGRPPGARPPHRGGGRGGGGGDAAAPPARGGGGGGKARPPGGGAAPCEPGCQCRAPMVSVSSERHPLYNRVKTGQIANCALPCHNPFFSQDERAFTVFWIGLWSVLCFVSTFATVSTFLIDMERFKYPERPIIFLSACYLFVSVGYLVRLVAGHEKVACSGGAPGAGGAGGAGGAAAGAGAAGAGAGGPGGRGEYEELGAVEQHVRYETTGPALCTVVFLLVYFFGMASSIWWVILSLTWFLAAGMKWGNEAIAGYSQYFHLAAWLVPSVKSIAVLALSSVDGDPVAGICYVGNQSLDNLRGFVLAPLVIYLFIGTMFLLAGFVSLFRIRSVIKQQDGPTKTHKLEKLMIRLGLFTVLYTVPAAVVVACLFYEQHNRPRWEATHNCPCLRDLQPDQARRPDYAVFMLKYFMCLVVGITSGVWVWSGKTLESWRSLCTRCCWASKGAAVGGGAGATAAGGGGGPGGGGGGGPGGGGGPGGGGGSLYSDVSTGLTWRSGTASSVSYPKQMPLSQV

Target ORF information:

RefSeq Version	NM_031866.2
Organism	Homo sapiens(human)
Definition	Homo sapiens frizzled class receptor 8 (FZD8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031866.2

ORF Insert Sequence:

ATGGAGTGGG GTTACCTGTT GGAAGTGACC TCGCTGCTGG CCGCCTTGGC GCTGCTGCAG 
CGCTCTAGCG GCGCTGCGGC CGCCTCGGCC AAGGAGCTGG CATGCCAAGA GATCACCGTG 
CCGCTGTGTA AGGGCATCGG CTACAACTAC ACCTACATGC CCAATCAGTT CAACCACGAC 
ACGCAAGACG AGGCGGGCCT GGAGGTGCAC CAGTTCTGGC CGCTGGTGGA GATCCAGTGC 
TCGCCCGATC TCAAGTTCTT CCTGTGCAGC ATGTACACGC CCATCTGCCT AGAGGACTAC 
AAGAAGCCGC TGCCGCCCTG CCGCTCGGTG TGCGAGCGCG CCAAGGCCGG CTGCGCGCCG 
CTCATGCGCC AGTACGGCTT CGCCTGGCCC GACCGCATGC GCTGCGACCG GCTGCCCGAG 
CAAGGCAACC CTGACACGCT GTGCATGGAC TACAACCGCA CCGACCTAAC CACCGCCGCG 
CCCAGCCCGC CGCGCCGCCT GCCGCCGCCG CCGCCCGGCG AGCAGCCGCC TTCGGGCAGC 
GGCCACGGCC GCCCGCCGGG GGCCAGGCCC CCGCACCGCG GCGGCGGCAG GGGCGGTGGC 
GGCGGGGACG CGGCGGCGCC CCCAGCTCGC GGCGGCGGCG GTGGCGGGAA GGCGCGGCCC 
CCTGGCGGCG GCGCGGCTCC CTGCGAGCCC GGGTGCCAGT GCCGCGCGCC TATGGTGAGC 
GTGTCCAGCG AGCGCCACCC GCTCTACAAC CGCGTCAAGA CAGGCCAGAT CGCTAACTGC 
GCGCTGCCCT GCCACAACCC CTTTTTCAGC CAGGACGAGC GCGCCTTCAC CGTCTTCTGG 
ATCGGCCTGT GGTCGGTGCT CTGCTTCGTG TCCACCTTCG CCACCGTCTC CACCTTCCTT 
ATCGACATGG AGCGCTTCAA GTACCCGGAG CGGCCCATTA TCTTCCTCTC GGCCTGCTAC 
CTCTTCGTGT CGGTGGGCTA CCTAGTGCGC CTGGTGGCGG GCCACGAGAA GGTGGCGTGC 
AGCGGTGGCG CGCCGGGCGC GGGGGGCGCT GGGGGCGCGG GCGGCGCGGC GGCGGGCGCG 
GGCGCGGCGG GCGCGGGCGC GGGCGGCCCG GGCGGGCGCG GCGAGTACGA GGAGCTGGGC 
GCGGTGGAGC AGCACGTGCG CTACGAGACC ACCGGCCCCG CGCTGTGCAC CGTGGTCTTC 
TTGCTGGTCT ACTTCTTCGG CATGGCCAGC TCCATCTGGT GGGTGATCTT GTCGCTCACA 
TGGTTCCTGG CGGCCGGTAT GAAGTGGGGC AACGAAGCCA TCGCCGGCTA CTCGCAGTAC 
TTCCACCTGG CCGCGTGGCT TGTGCCCAGC GTCAAGTCCA TCGCGGTGCT GGCGCTCAGC 
TCGGTGGACG GCGACCCGGT GGCGGGCATC TGCTACGTGG GCAACCAGAG CCTGGACAAC 
CTGCGCGGCT TCGTGCTGGC GCCGCTGGTC ATCTACCTCT TCATCGGCAC CATGTTCCTG 
CTGGCCGGCT TCGTGTCCCT CTTCCGCATC CGCTCGGTCA TCAAGCAACA GGACGGCCCC 
ACCAAGACGC ACAAGCTGGA GAAGCTGATG ATCCGCCTGG GCCTGTTCAC CGTGCTCTAC 
ACCGTGCCCG CCGCGGTGGT GGTCGCCTGC CTCTTCTACG AGCAGCACAA CCGCCCGCGC 
TGGGAGGCCA CGCACAACTG CCCGTGCCTG CGGGACCTGC AGCCCGACCA GGCACGCAGG 
CCCGACTACG CCGTCTTCAT GCTCAAGTAC TTCATGTGCC TAGTGGTGGG CATCACCTCG 
GGCGTGTGGG TCTGGTCCGG CAAGACGCTG GAGTCCTGGC GCTCCCTGTG CACCCGCTGC 
TGCTGGGCCA GCAAGGGCGC CGCGGTGGGC GGGGGCGCGG GCGCCACGGC CGCGGGGGGT 
GGCGGCGGGC CGGGGGGCGG CGGCGGCGGG GGACCCGGCG GCGGCGGGGG GCCGGGCGGC 
GGCGGGGGCT CCCTCTACAG CGACGTCAGC ACTGGCCTGA CGTGGCGGTC GGGCACGGCG 
AGCTCCGTGT CTTATCCAAA GCAGATGCCA TTGTCCCAGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Wnt receptor Frizzled 8 is a target of ERG in prostate cancer.
The Prostate78(16)1311-1320(2018 12)
Chakravarthi BVSK,Chandrashekar DS,Hodigere Balasubramanya SA,Robinson AD,Carskadon S,Rao U,Gordetsky J,Manne U,Netto GJ,Sudarshan S,Palanisamy N,Varambally S

Frizzled-8 integrates Wnt-11 and transforming growth factor-β signaling in prostate cancer.
Nature communications9(1)1747(2018 05)
Murillo-Garzón V,Gorroño-Etxebarria I,Åkerfelt M,Puustinen MC,Sistonen L,Nees M,Carton J,Waxman J,Kypta RM

MicroRNA-520b Suppresses Proliferation, Migration, and Invasion of Spinal Osteosarcoma Cells via Downregulation of Frizzled-8.
Oncology research25(8)1297-1304(2017 Sep)
Wang J,Pang W,Zuo Z,Zhang W,He W

FZD8, a target of p53, promotes bone metastasis in prostate cancer by activating canonical Wnt/β-catenin signaling.
Cancer letters402166-176(2017 08)
Li Q,Ye L,Zhang X,Wang M,Lin C,Huang S,Guo W,Lai Y,Du H,Li J,Song L,Peng X

Insights into Wnt binding and signalling from the structures of two Frizzled cysteine-rich domains.
Nature412(6842)86-90(2001 Jul)
Dann CE,Hsieh JC,Rattner A,Sharma D,Nathans J,Leahy DJ