fzd8a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	fzd8a
Entrez Gene ID	58073
Full Name	frizzled class receptor 8a
Synonyms	fz5,fz8a,fzc,wu:fd02c05,zg05
General protein information	Preferred Names frizzled class receptor 8a Names frizzled-8 frizzled homolog 8a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_130918.2	NP_570993.2	frizzled-8

Pathways from BioSystems

Homologies

Homo sapiens (human)	FZD8	NP_114072.1
Mus musculus (house mouse)	Fzd8	NP_032084.2
Rattus norvegicus (Norway rat)	Fzd8	NP_001037716.1
Rattus norvegicus (Norway rat)	LOC100909849	XP_003751769.1
Xenopus tropicalis (tropical clawed frog)	fzd8	NP_001090860.2
Canis lupus familiaris (dog)	FZD8	XP_005617125.1
Caenorhabditis elegans (roundworm)	cfz-2	NP_503965.2
Pan troglodytes (chimpanzee)	FZD8	XP_001142144.2
Danio rerio (zebrafish)	fzd8a	NP_570993.2
Macaca mulatta (Rhesus monkey)	FZD8	XP_001096837.1
Bos taurus (cattle)	FZD8	XP_005214377.1

Gene Ontology
Bibliography

Related articles in PubMed

EGFR is required for Wnt9a-Fzd9b signalling specificity in haematopoietic stem cells.
Grainger S, Nguyen N, Richter J, Setayesh J, Lonquich B, Oon CH, Wozniak JM, Barahona R, Kamei CN, Houston J, Carrillo-Terrazas M, Drummond IA, Gonzalez D, Willert K, Traver D
Nature cell biology21(6)721-730(2019 06)

Wnt signaling mediates new nephron formation during zebrafish kidney regeneration.
Kamei CN, Gallegos TF, Liu Y, Hukriede N, Drummond IA
Development (Cambridge, England)146(8)(2019 04)

Wnt/β-catenin signaling controls intrahepatic biliary network formation in zebrafish by regulating notch activity.
So J, Khaliq M, Evason K, Ninov N, Martin BL, Stainier DYR, Shin D
Hepatology (Baltimore, Md.)67(6)2352-2366(2018 06)

Mutation of frizzled8a delays neural retinal cell differentiation and results in microphthalmia in zebrafish.
Cheng XN, Shao M, Shi DL
The International journal of developmental biology62(4-5)285-291(2018)

Dissecting the role of Wnt signaling and its interactions with FGF signaling during midbrain neurogenesis.
Dyer C, Blanc E, Stanley RJ, Knight RD
Neurogenesis (Austin, Tex.)2(1)e1057313(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following fzd8a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fzd8a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02500	NM_130918.2 Latest version!	Danio rerio frizzled class receptor 8a (fzd8a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fzd8a ( NM_130918.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_570993.2 Danio rerio frizzled class receptor 8a (fzd8a), mRNA.

Price & Availability↑

CloneID	ODa02500
Clone ID Related Accession (Same CDS sequence)	NM_130918.2
Accession Version	NM_130918.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575648000000
Organism	Danio rerio(zebrafish)
Product	frizzled-8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC056273.1. On or before Nov 28, 2007 this sequence version replaced NM_131137.1, NM_131554.1, NM_130918.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC056273.1 [ECO:0000332] ##Evidence-Data-END##

ATGGAGTGCT ACCTGTTGGG GATTTACCTG TTCCTCGCGC TGGCTCTGCT GCCCCGATCG 
AGCGGTACCA CGGCCAAGGA GATCACCTGT CAGGAGATCG CCGTTCCGCT GTGCAAGGGC 
ATCGGTTATA ACTACACCTA CATGCCCAAC CAGTTCAACC ACGACACGCA GGATGAAGCC 
GGCTTGGAGG TGCACCAGTT CTGGCCTCTG GTGGAGATTC AGTGCTCCCC GGATCTCAAG 
TTCTTCTTGT GCAGCATGTA CACCCCGATA TGCCTGGAGG ACTATAAGAA ACCCCTTCCG 
CCGTGCCGGA GCGTCTGCGA GAGAGCCAAG GCGGGCTGCG CCCCGCTCAT GCGGCAATAC 
GGCTTCCCTT GGCCGGATCG GATGAGGTGC GATCTACTGC CCGTGCAAGG AGCCCCAGAC 
ACGCTGTGCA TGGACTACAA CCGAACCGAC TCCACCACAG TGTCGCCGGT GCTGTCCAAA 
CCCACCAACT ACCCCAGCAA AGCCATTAAT CCGCACAAAA AGAAAAGCGG GCGGCCAGGC 
GTCGGACCCA ATAAGAATAA GCCCTGCGAG CCGGGCTGCC AGTGTCGCGC GCCGATGGTG 
CCGGTGAACA GCGATCGACA CCCGCTCTAC AACCGCGTTA AGACGGGTCA AATCCCAAAC 
TGCGCCATGC CATGTCACAA CCCATATTTT ACGCAGGATG AGCGGACTTT TACGGCCTTC 
TGGATCGGAC TTTGGTCCGT GTTGTGCTTC ATATCCACCT TCGCCACAGT CGCCACCTTC 
CTAATCGACA TGGAGCGGTT TAAATACCCA GAGCGCCCTA TTATTTTCCT CTCGGCGTGC 
TACATGTTTG TGTCGATCGG GTATATAGTG CGATTGATCG CCGGGCACGA AAAAGTGGCG 
TGCAATCGGG AGTATGACGT GGAGCACATT CACTACGAGA CCACCGGCCC CGCGTTGTGC 
ACCGTTGTGT TCCTGCTTAT CTACTTCTTC GGAATGGCCA GCTCCATATG GTGGGTCATC 
CTCTCGCTCA CCTGGTTCCT CGCGGCGGGC ATGAAGTGGG GAAACGAGGC CATCGCGGGC 
TACTCTCAGT ACTTTCACCT GGCCGCTTGG CTCATCCCGT CCATGAAGTC TATAGCCGTC 
CTCGCGCTGA GCTCGGTGGA CGGAGATCCG GTCGCCGGGA TCTGCTACGT TGGCAACCAG 
AACTTGGACA ATCTGCGGGG CTTCGTGCTG GCCCCCCTGG TCATATACCT ATTTATAGGC 
ACCATGTTTC TTTTGGCTGG ATTTGTGTCG CTTTTTAGGA TCAGGAGTGT CATTAAACAG 
GGGGGCACTA AAACGGACAA GCTGGAGAAG CTGATGATCC GAATTGGGAT TTTCACGGTG 
CTCTACACGG TTCCCGCCAC CATCATCGTG GCGTGTTATT TCTATGAGCA GCACAACAGA 
CAGAGTTGGG AGATCACTCA TAACTGTTCG TGTTTACTGG AGCAGGAGAT CAAGAGGCCG 
GACTATGCCG TCTTCATGCT CAAATACTTC ATGTGCCTTC TGGTGGGCAT CACCTCTGGA 
GTTTGGACCT GGTCCGGTAA GACATTGGAG TCCTGGAGGA GTTTCTGCAC GCGCTGCTGT 
TGGGGCAGCA AGGGCTCCGG TGGCTCCATG TACAGTGACG TGAGCACGGG ATTAACGTGG 
AGGTCCGGTA CCGCCAGCTC CGTGTCGTGC CCCAAGCAGA TGCCTTTGTC CCAAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_570993.2
CDS	315..2054
Translation	MECYLLGIYLFLALALLPRSSGTTAKEITCQEIAVPLCKGIGYNYTYMPNQFNHDTQDEAGLEVHQFWPLVEIQCSPDLKFFLCSMYTPICLEDYKKPLPPCRSVCERAKAGCAPLMRQYGFPWPDRMRCDLLPVQGAPDTLCMDYNRTDSTTVSPVLSKPTNYPSKAINPHKKKSGRPGVGPNKNKPCEPGCQCRAPMVPVNSDRHPLYNRVKTGQIPNCAMPCHNPYFTQDERTFTAFWIGLWSVLCFISTFATVATFLIDMERFKYPERPIIFLSACYMFVSIGYIVRLIAGHEKVACNREYDVEHIHYETTGPALCTVVFLLIYFFGMASSIWWVILSLTWFLAAGMKWGNEAIAGYSQYFHLAAWLIPSMKSIAVLALSSVDGDPVAGICYVGNQNLDNLRGFVLAPLVIYLFIGTMFLLAGFVSLFRIRSVIKQGGTKTDKLEKLMIRIGIFTVLYTVPATIIVACYFYEQHNRQSWEITHNCSCLLEQEIKRPDYAVFMLKYFMCLLVGITSGVWTWSGKTLESWRSFCTRCCWGSKGSGGSMYSDVSTGLTWRSGTASSVSCPKQMPLSQV

Target ORF information:

RefSeq Version	NM_130918.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio frizzled class receptor 8a (fzd8a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130918.2

ORF Insert Sequence:

ATGGAGTGCT ACCTGTTGGG GATTTACCTG TTCCTCGCGC TGGCTCTGCT GCCCCGATCG 
AGCGGTACCA CGGCCAAGGA GATCACCTGT CAGGAGATCG CCGTTCCGCT GTGCAAGGGC 
ATCGGTTATA ACTACACCTA CATGCCCAAC CAGTTCAACC ACGACACGCA GGATGAAGCC 
GGCTTGGAGG TGCACCAGTT CTGGCCTCTG GTGGAGATTC AGTGCTCCCC GGATCTCAAG 
TTCTTCTTGT GCAGCATGTA CACCCCGATA TGCCTGGAGG ACTATAAGAA ACCCCTTCCG 
CCGTGCCGGA GCGTCTGCGA GAGAGCCAAG GCGGGCTGCG CCCCGCTCAT GCGGCAATAC 
GGCTTCCCTT GGCCGGATCG GATGAGGTGC GATCTACTGC CCGTGCAAGG AGCCCCAGAC 
ACGCTGTGCA TGGACTACAA CCGAACCGAC TCCACCACAG TGTCGCCGGT GCTGTCCAAA 
CCCACCAACT ACCCCAGCAA AGCCATTAAT CCGCACAAAA AGAAAAGCGG GCGGCCAGGC 
GTCGGACCCA ATAAGAATAA GCCCTGCGAG CCGGGCTGCC AGTGTCGCGC GCCGATGGTG 
CCGGTGAACA GCGATCGACA CCCGCTCTAC AACCGCGTTA AGACGGGTCA AATCCCAAAC 
TGCGCCATGC CATGTCACAA CCCATATTTT ACGCAGGATG AGCGGACTTT TACGGCCTTC 
TGGATCGGAC TTTGGTCCGT GTTGTGCTTC ATATCCACCT TCGCCACAGT CGCCACCTTC 
CTAATCGACA TGGAGCGGTT TAAATACCCA GAGCGCCCTA TTATTTTCCT CTCGGCGTGC 
TACATGTTTG TGTCGATCGG GTATATAGTG CGATTGATCG CCGGGCACGA AAAAGTGGCG 
TGCAATCGGG AGTATGACGT GGAGCACATT CACTACGAGA CCACCGGCCC CGCGTTGTGC 
ACCGTTGTGT TCCTGCTTAT CTACTTCTTC GGAATGGCCA GCTCCATATG GTGGGTCATC 
CTCTCGCTCA CCTGGTTCCT CGCGGCGGGC ATGAAGTGGG GAAACGAGGC CATCGCGGGC 
TACTCTCAGT ACTTTCACCT GGCCGCTTGG CTCATCCCGT CCATGAAGTC TATAGCCGTC 
CTCGCGCTGA GCTCGGTGGA CGGAGATCCG GTCGCCGGGA TCTGCTACGT TGGCAACCAG 
AACTTGGACA ATCTGCGGGG CTTCGTGCTG GCCCCCCTGG TCATATACCT ATTTATAGGC 
ACCATGTTTC TTTTGGCTGG ATTTGTGTCG CTTTTTAGGA TCAGGAGTGT CATTAAACAG 
GGGGGCACTA AAACGGACAA GCTGGAGAAG CTGATGATCC GAATTGGGAT TTTCACGGTG 
CTCTACACGG TTCCCGCCAC CATCATCGTG GCGTGTTATT TCTATGAGCA GCACAACAGA 
CAGAGTTGGG AGATCACTCA TAACTGTTCG TGTTTACTGG AGCAGGAGAT CAAGAGGCCG 
GACTATGCCG TCTTCATGCT CAAATACTTC ATGTGCCTTC TGGTGGGCAT CACCTCTGGA 
GTTTGGACCT GGTCCGGTAA GACATTGGAG TCCTGGAGGA GTTTCTGCAC GCGCTGCTGT 
TGGGGCAGCA AGGGCTCCGG TGGCTCCATG TACAGTGACG TGAGCACGGG ATTAACGTGG 
AGGTCCGGTA CCGCCAGCTC CGTGTCGTGC CCCAAGCAGA TGCCTTTGTC CCAAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

EGFR is required for Wnt9a-Fzd9b signalling specificity in haematopoietic stem cells.
Nature cell biology21(6)721-730(2019 06)
Grainger S,Nguyen N,Richter J,Setayesh J,Lonquich B,Oon CH,Wozniak JM,Barahona R,Kamei CN,Houston J,Carrillo-Terrazas M,Drummond IA,Gonzalez D,Willert K,Traver D

Wnt signaling mediates new nephron formation during zebrafish kidney regeneration.
Development (Cambridge, England)146(8)(2019 04)
Kamei CN,Gallegos TF,Liu Y,Hukriede N,Drummond IA

Wnt/β-catenin signaling controls intrahepatic biliary network formation in zebrafish by regulating notch activity.
Hepatology (Baltimore, Md.)67(6)2352-2366(2018 06)
So J,Khaliq M,Evason K,Ninov N,Martin BL,Stainier DYR,Shin D

Mutation of frizzled8a delays neural retinal cell differentiation and results in microphthalmia in zebrafish.
The International journal of developmental biology62(4-5)285-291(2018)
Cheng XN,Shao M,Shi DL

Dissecting the role of Wnt signaling and its interactions with FGF signaling during midbrain neurogenesis.
Neurogenesis (Austin, Tex.)2(1)e1057313(2015)
Dyer C,Blanc E,Stanley RJ,Knight RD