RPL27 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RPL27
Entrez Gene ID	6155
Full Name	ribosomal protein L27
Synonyms	DBA16,L27
General protein information	Preferred Names ribosomal protein L27 Names 60S ribosomal protein L27 large ribosomal subunit protein eL27
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q21.31
Summary	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L27e family of ribosomal proteins and a component of the 60S subunit. A splice site mutation in this gene has been identified in a Diamond-Blackfan anemia (DBA) patient. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Mar 2017].
Disorder MIM:	607526
Disorder Html:	?Diamond-Blackfan anemia 16, 617408 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000988.5	NP_000979.1	60S ribosomal protein L27
NM_001349921.2	NP_001336850.1	60S ribosomal protein L27
NM_001349921.1	NP_001336850.1	60S ribosomal protein L27
NM_000988.3	NP_000979.1	60S ribosomal protein L27
NM_000988.4	NP_000979.1	60S ribosomal protein L27
NM_001349922.1	NP_001336851.1	60S ribosomal protein L27
NM_001349922.2	NP_001336851.1	60S ribosomal protein L27

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0E03455g	XP_454100.1
Magnaporthe oryzae (rice blast fungus)	MGG_06693	XP_003709402.1
Mus musculus (house mouse)	Rpl27	NP_035419.1
Pan troglodytes (chimpanzee)	RPL27	XP_001159277.1
Canis lupus familiaris (dog)	RPL27	NP_001003102.2
Bos taurus (cattle)	RPL27	NP_001029223.1
Gallus gallus (chicken)	RPL27	NP_990668.1
Homo sapiens (human)	RPL27	NP_000979.1
Saccharomyces cerevisiae (baker's yeast)	RPL27B	NP_010759.1
Arabidopsis thaliana (thale cress)	AT2G32220	NP_180781.1
Arabidopsis thaliana (thale cress)	AT4G15000	NP_193236.1
Oryza sativa (rice)	Os02g0284600	NP_001046570.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011687	XP_552567.1
Schizosaccharomyces pombe (fission yeast)	rpl2702	NP_588378.1
Neurospora crassa	NCU01827	XP_956611.1
Danio rerio (zebrafish)	rpl27	NP_956018.1
Caenorhabditis elegans (roundworm)	rpl-27	NP_490905.1
Oryza sativa (rice)	Os10g0564300	NP_001065410.1
Eremothecium gossypii	AGOS_AFR413C	NP_985960.1
Schizosaccharomyces pombe (fission yeast)	rpl2701	NP_596141.1
Macaca mulatta (Rhesus monkey)	RPL27	XP_001112742.1
Rattus norvegicus (Norway rat)	Rpl27	NP_071959.1
Drosophila melanogaster (fruit fly)	RpL27	NP_651417.1
Saccharomyces cerevisiae (baker's yeast)	RPL27A	NP_011874.1
Arabidopsis thaliana (thale cress)	AT3G22230	NP_188862.1
Xenopus tropicalis (tropical clawed frog)	rpl27	NP_001016180.1

Gene Ontology
Bibliography

Related articles in PubMed

The DHX33 RNA Helicase Promotes mRNA Translation Initiation.
Zhang Y, You J, Wang X, Weber J
Molecular and cellular biology35(17)2918-31(2015 Sep)

Loss of function mutations in RPL27 and RPS27 identified by whole-exome sequencing in Diamond-Blackfan anaemia.
Wang R, Yoshida K, Toki T, Sawada T, Uechi T, Okuno Y, Sato-Otsubo A, Kudo K, Kamimaki I, Kanezaki R, Shiraishi Y, Chiba K, Tanaka H, Terui K, Sato T, Iribe Y, Ohga S, Kuramitsu M, Hamaguchi I, Ohara A, Hara J, Goi K, Matsubara K, Koike K, Ishiguro A, Okamoto Y, Watanabe K, Kanno H, Kojima S, Miyano S, Kenmochi N, Ogawa S, Ito E
British journal of haematology168(6)854-64(2015 Mar)

SILAC-based proteomics of human primary endothelial cell morphogenesis unveils tumor angiogenic markers.
Zanivan S, Maione F, Hein MY, Hernández-Fernaud JR, Ostasiewicz P, Giraudo E, Mann M
Molecular & cellular proteomics : MCP12(12)3599-611(2013 Dec)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanhäusser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RPL27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPL27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30475	NM_000988.5 Latest version!	Homo sapiens ribosomal protein L27 (RPL27), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu30475	NM_001349922.2 Latest version!	Homo sapiens ribosomal protein L27 (RPL27), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu30475	NM_001349921.2 Latest version!	Homo sapiens ribosomal protein L27 (RPL27), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
View Old Accession Versions >>
OHu30475	NM_001349921.1	Homo sapiens ribosomal protein L27 (RPL27), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu30475	NM_000988.3	Homo sapiens ribosomal protein L27 (RPL27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu30475	NM_000988.4	Homo sapiens ribosomal protein L27 (RPL27), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu30475	NM_001349922.1	Homo sapiens ribosomal protein L27 (RPL27), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPL27 ( NM_000988.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000979.1 Homo sapiens ribosomal protein L27 (RPL27), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu30475
Clone ID Related Accession (Same CDS sequence)	NM_000988.5 , NM_001349921.2 , NM_001349921.1 , NM_000988.3 , NM_000988.4 , NM_001349922.1 , NM_001349922.2
Accession Version	NM_000988.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570032000000
Organism	Homo sapiens(human)
Product	60S ribosomal protein L27
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from AC055866.19. This sequence is a reference standard in the RefSeqGene project. On May 8, 2018 this sequence version replaced NM_000988.4. Transcript Variant: This variant (1) encodes the protein. Variants 1-3 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BG618850.1, SRR1163657.220571.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCAAGT TCATGAAACC TGGGAAGGTG GTGCTTGTCC TGGCTGGACG CTACTCCGGA 
CGCAAAGCTG TCATCGTGAA GAACATTGAT GATGGCACCT CAGATCGCCC CTACAGCCAT 
GCTCTGGTGG CTGGAATTGA CCGCTACCCC CGCAAAGTGA CAGCTGCCAT GGGCAAGAAG 
AAGATCGCCA AGAGATCAAA GATAAAATCT TTTGTGAAAG TGTATAACTA CAATCACCTA 
ATGCCCACAA GGTACTCTGT GGATATCCCC TTGGACAAAA CTGTCGTCAA TAAGGATGTC 
TTCAGAGATC CTGCTCTTAA ACGCAAGGCC CGACGGGAGG CCAAGGTCAA GTTTGAAGAG 
AGATACAAGA CAGGCAAGAA CAAGTGGTTC TTCCAGAAAC TGCGGTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000979.1
CDS	56..466
Translation	MGKFMKPGKVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF

Target ORF information:

RefSeq Version	NM_000988.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L27 (RPL27), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000988.5

ORF Insert Sequence:

ATGGGCAAGT TCATGAAACC TGGGAAGGTG GTGCTTGTCC TGGCTGGACG CTACTCCGGA 
CGCAAAGCTG TCATCGTGAA GAACATTGAT GATGGCACCT CAGATCGCCC CTACAGCCAT 
GCTCTGGTGG CTGGAATTGA CCGCTACCCC CGCAAAGTGA CAGCTGCCAT GGGCAAGAAG 
AAGATCGCCA AGAGATCAAA GATAAAATCT TTTGTGAAAG TGTATAACTA CAATCACCTA 
ATGCCCACAA GGTACTCTGT GGATATCCCC TTGGACAAAA CTGTCGTCAA TAAGGATGTC 
TTCAGAGATC CTGCTCTTAA ACGCAAGGCC CGACGGGAGG CCAAGGTCAA GTTTGAAGAG 
AGATACAAGA CAGGCAAGAA CAAGTGGTTC TTCCAGAAAC TGCGGTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL27 ( NM_001349921.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336850.1 Homo sapiens ribosomal protein L27 (RPL27), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu30475
Clone ID Related Accession (Same CDS sequence)	NM_000988.5 , NM_001349921.2 , NM_001349921.1 , NM_000988.3 , NM_000988.4 , NM_001349922.1 , NM_001349922.2
Accession Version	NM_001349921.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562601600000
Organism	Homo sapiens(human)
Product	60S ribosomal protein L27
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP203677.1 and BC098560.1. On Jun 2, 2019 this sequence version replaced NM_001349921.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP203677.1, SRR1163655.650159.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

ATGGGCAAGT TCATGAAACC TGGGAAGGTG GTGCTTGTCC TGGCTGGACG CTACTCCGGA 
CGCAAAGCTG TCATCGTGAA GAACATTGAT GATGGCACCT CAGATCGCCC CTACAGCCAT 
GCTCTGGTGG CTGGAATTGA CCGCTACCCC CGCAAAGTGA CAGCTGCCAT GGGCAAGAAG 
AAGATCGCCA AGAGATCAAA GATAAAATCT TTTGTGAAAG TGTATAACTA CAATCACCTA 
ATGCCCACAA GGTACTCTGT GGATATCCCC TTGGACAAAA CTGTCGTCAA TAAGGATGTC 
TTCAGAGATC CTGCTCTTAA ACGCAAGGCC CGACGGGAGG CCAAGGTCAA GTTTGAAGAG 
AGATACAAGA CAGGCAAGAA CAAGTGGTTC TTCCAGAAAC TGCGGTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336850.1
CDS	146..556
Translation	MGKFMKPGKVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF

Target ORF information:

RefSeq Version	NM_001349921.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L27 (RPL27), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001349921.2

ORF Insert Sequence:

ATGGGCAAGT TCATGAAACC TGGGAAGGTG GTGCTTGTCC TGGCTGGACG CTACTCCGGA 
CGCAAAGCTG TCATCGTGAA GAACATTGAT GATGGCACCT CAGATCGCCC CTACAGCCAT 
GCTCTGGTGG CTGGAATTGA CCGCTACCCC CGCAAAGTGA CAGCTGCCAT GGGCAAGAAG 
AAGATCGCCA AGAGATCAAA GATAAAATCT TTTGTGAAAG TGTATAACTA CAATCACCTA 
ATGCCCACAA GGTACTCTGT GGATATCCCC TTGGACAAAA CTGTCGTCAA TAAGGATGTC 
TTCAGAGATC CTGCTCTTAA ACGCAAGGCC CGACGGGAGG CCAAGGTCAA GTTTGAAGAG 
AGATACAAGA CAGGCAAGAA CAAGTGGTTC TTCCAGAAAC TGCGGTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL27 ( NM_001349921.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336850.1 Homo sapiens ribosomal protein L27 (RPL27), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu30475
Clone ID Related Accession (Same CDS sequence)	NM_000988.5 , NM_001349921.2 , NM_001349921.1 , NM_000988.3 , NM_000988.4 , NM_001349922.1 , NM_001349922.2
Accession Version	NM_001349921.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550937600000
Organism	Homo sapiens(human)
Product	60S ribosomal protein L27
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP203677.1 and BC098560.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP203677.1, SRR1803617.181991.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

ATGGGCAAGT TCATGAAACC TGGGAAGGTG GTGCTTGTCC TGGCTGGACG CTACTCCGGA 
CGCAAAGCTG TCATCGTGAA GAACATTGAT GATGGCACCT CAGATCGCCC CTACAGCCAT 
GCTCTGGTGG CTGGAATTGA CCGCTACCCC CGCAAAGTGA CAGCTGCCAT GGGCAAGAAG 
AAGATCGCCA AGAGATCAAA GATAAAATCT TTTGTGAAAG TGTATAACTA CAATCACCTA 
ATGCCCACAA GGTACTCTGT GGATATCCCC TTGGACAAAA CTGTCGTCAA TAAGGATGTC 
TTCAGAGATC CTGCTCTTAA ACGCAAGGCC CGACGGGAGG CCAAGGTCAA GTTTGAAGAG 
AGATACAAGA CAGGCAAGAA CAAGTGGTTC TTCCAGAAAC TGCGGTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336850.1
CDS	146..556
Translation	MGKFMKPGKVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF

Target ORF information:

RefSeq Version	NM_001349921.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L27 (RPL27), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001349921.1

ORF Insert Sequence:

ATGGGCAAGT TCATGAAACC TGGGAAGGTG GTGCTTGTCC TGGCTGGACG CTACTCCGGA 
CGCAAAGCTG TCATCGTGAA GAACATTGAT GATGGCACCT CAGATCGCCC CTACAGCCAT 
GCTCTGGTGG CTGGAATTGA CCGCTACCCC CGCAAAGTGA CAGCTGCCAT GGGCAAGAAG 
AAGATCGCCA AGAGATCAAA GATAAAATCT TTTGTGAAAG TGTATAACTA CAATCACCTA 
ATGCCCACAA GGTACTCTGT GGATATCCCC TTGGACAAAA CTGTCGTCAA TAAGGATGTC 
TTCAGAGATC CTGCTCTTAA ACGCAAGGCC CGACGGGAGG CCAAGGTCAA GTTTGAAGAG 
AGATACAAGA CAGGCAAGAA CAAGTGGTTC TTCCAGAAAC TGCGGTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL27 ( NM_000988.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000979.1 Homo sapiens ribosomal protein L27 (RPL27), mRNA.

Price & Availability↑

CloneID	OHu30475
Clone ID Related Accession (Same CDS sequence)	NM_000988.5 , NM_001349921.2 , NM_001349921.1 , NM_000988.3 , NM_000988.4 , NM_001349922.1 , NM_001349922.2
Accession Version	NM_000988.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426262400000
Organism	Homo sapiens(Human)
Product	60S ribosomal protein L27
Comment

ATGGGCAAGT TCATGAAACC TGGGAAGGTG GTGCTTGTCC TGGCTGGACG CTACTCCGGA 
CGCAAAGCTG TCATCGTGAA GAACATTGAT GATGGCACCT CAGATCGCCC CTACAGCCAT 
GCTCTGGTGG CTGGAATTGA CCGCTACCCC CGCAAAGTGA CAGCTGCCAT GGGCAAGAAG 
AAGATCGCCA AGAGATCAAA GATAAAATCT TTTGTGAAAG TGTATAACTA CAATCACCTA 
ATGCCCACAA GGTACTCTGT GGATATCCCC TTGGACAAAA CTGTCGTCAA TAAGGATGTC 
TTCAGAGATC CTGCTCTTAA ACGCAAGGCC CGACGGGAGG CCAAGGTCAA GTTTGAAGAG 
AGATACAAGA CAGGCAAGAA CAAGTGGTTC TTCCAGAAAC TGCGGTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000979.1
Translation	MGKFMKPGKVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF

Target ORF information:

RefSeq Version	NM_000988.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens ribosomal protein L27 (RPL27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000988.3

ORF Insert Sequence:

ATGGGCAAGT TCATGAAACC TGGGAAGGTG GTGCTTGTCC TGGCTGGACG CTACTCCGGA 
CGCAAAGCTG TCATCGTGAA GAACATTGAT GATGGCACCT CAGATCGCCC CTACAGCCAT 
GCTCTGGTGG CTGGAATTGA CCGCTACCCC CGCAAAGTGA CAGCTGCCAT GGGCAAGAAG 
AAGATCGCCA AGAGATCAAA GATAAAATCT TTTGTGAAAG TGTATAACTA CAATCACCTA 
ATGCCCACAA GGTACTCTGT GGATATCCCC TTGGACAAAA CTGTCGTCAA TAAGGATGTC 
TTCAGAGATC CTGCTCTTAA ACGCAAGGCC CGACGGGAGG CCAAGGTCAA GTTTGAAGAG 
AGATACAAGA CAGGCAAGAA CAAGTGGTTC TTCCAGAAAC TGCGGTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL27 ( NM_000988.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000979.1 Homo sapiens ribosomal protein L27 (RPL27), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu30475
Clone ID Related Accession (Same CDS sequence)	NM_000988.5 , NM_001349921.2 , NM_001349921.1 , NM_000988.3 , NM_000988.4 , NM_001349922.1 , NM_001349922.2
Accession Version	NM_000988.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1514304000000
Organism	Homo sapiens(human)
Product	60S ribosomal protein L27
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from CD172658.1, BU955518.1 and BC098560.1. This sequence is a reference standard in the RefSeqGene project. On Mar 30, 2017 this sequence version replaced NM_000988.3. Transcript Variant: This variant (1) encodes the protein. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CD173501.1, SRR1163657.196696.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGCAAGT TCATGAAACC TGGGAAGGTG GTGCTTGTCC TGGCTGGACG CTACTCCGGA 
CGCAAAGCTG TCATCGTGAA GAACATTGAT GATGGCACCT CAGATCGCCC CTACAGCCAT 
GCTCTGGTGG CTGGAATTGA CCGCTACCCC CGCAAAGTGA CAGCTGCCAT GGGCAAGAAG 
AAGATCGCCA AGAGATCAAA GATAAAATCT TTTGTGAAAG TGTATAACTA CAATCACCTA 
ATGCCCACAA GGTACTCTGT GGATATCCCC TTGGACAAAA CTGTCGTCAA TAAGGATGTC 
TTCAGAGATC CTGCTCTTAA ACGCAAGGCC CGACGGGAGG CCAAGGTCAA GTTTGAAGAG 
AGATACAAGA CAGGCAAGAA CAAGTGGTTC TTCCAGAAAC TGCGGTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000979.1
CDS	84..494
Translation	MGKFMKPGKVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF

Target ORF information:

RefSeq Version	NM_000988.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L27 (RPL27), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000988.4

ORF Insert Sequence:

ATGGGCAAGT TCATGAAACC TGGGAAGGTG GTGCTTGTCC TGGCTGGACG CTACTCCGGA 
CGCAAAGCTG TCATCGTGAA GAACATTGAT GATGGCACCT CAGATCGCCC CTACAGCCAT 
GCTCTGGTGG CTGGAATTGA CCGCTACCCC CGCAAAGTGA CAGCTGCCAT GGGCAAGAAG 
AAGATCGCCA AGAGATCAAA GATAAAATCT TTTGTGAAAG TGTATAACTA CAATCACCTA 
ATGCCCACAA GGTACTCTGT GGATATCCCC TTGGACAAAA CTGTCGTCAA TAAGGATGTC 
TTCAGAGATC CTGCTCTTAA ACGCAAGGCC CGACGGGAGG CCAAGGTCAA GTTTGAAGAG 
AGATACAAGA CAGGCAAGAA CAAGTGGTTC TTCCAGAAAC TGCGGTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL27 ( NM_001349922.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336851.1 Homo sapiens ribosomal protein L27 (RPL27), transcript variant 3, mRNA.

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CloneID	OHu30475
Clone ID Related Accession (Same CDS sequence)	NM_000988.5 , NM_001349921.2 , NM_001349921.1 , NM_000988.3 , NM_000988.4 , NM_001349922.1 , NM_001349922.2
Accession Version	NM_001349922.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1551024000000
Organism	Homo sapiens(human)
Product	60S ribosomal protein L27
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AW404176.1, CN302602.1 and BC098560.1. Transcript Variant: This variant (3) retains an intron in its 5' UTR compared to variant 1. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.434474.1, SRR1163658.452410.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGGCAAGT TCATGAAACC TGGGAAGGTG GTGCTTGTCC TGGCTGGACG CTACTCCGGA 
CGCAAAGCTG TCATCGTGAA GAACATTGAT GATGGCACCT CAGATCGCCC CTACAGCCAT 
GCTCTGGTGG CTGGAATTGA CCGCTACCCC CGCAAAGTGA CAGCTGCCAT GGGCAAGAAG 
AAGATCGCCA AGAGATCAAA GATAAAATCT TTTGTGAAAG TGTATAACTA CAATCACCTA 
ATGCCCACAA GGTACTCTGT GGATATCCCC TTGGACAAAA CTGTCGTCAA TAAGGATGTC 
TTCAGAGATC CTGCTCTTAA ACGCAAGGCC CGACGGGAGG CCAAGGTCAA GTTTGAAGAG 
AGATACAAGA CAGGCAAGAA CAAGTGGTTC TTCCAGAAAC TGCGGTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336851.1
CDS	287..697
Translation	MGKFMKPGKVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF

Target ORF information:

RefSeq Version	NM_001349922.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L27 (RPL27), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001349922.1

ORF Insert Sequence:

ATGGGCAAGT TCATGAAACC TGGGAAGGTG GTGCTTGTCC TGGCTGGACG CTACTCCGGA 
CGCAAAGCTG TCATCGTGAA GAACATTGAT GATGGCACCT CAGATCGCCC CTACAGCCAT 
GCTCTGGTGG CTGGAATTGA CCGCTACCCC CGCAAAGTGA CAGCTGCCAT GGGCAAGAAG 
AAGATCGCCA AGAGATCAAA GATAAAATCT TTTGTGAAAG TGTATAACTA CAATCACCTA 
ATGCCCACAA GGTACTCTGT GGATATCCCC TTGGACAAAA CTGTCGTCAA TAAGGATGTC 
TTCAGAGATC CTGCTCTTAA ACGCAAGGCC CGACGGGAGG CCAAGGTCAA GTTTGAAGAG 
AGATACAAGA CAGGCAAGAA CAAGTGGTTC TTCCAGAAAC TGCGGTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL27 ( NM_001349922.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336851.1 Homo sapiens ribosomal protein L27 (RPL27), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu30475
Clone ID Related Accession (Same CDS sequence)	NM_000988.5 , NM_001349921.2 , NM_001349921.1 , NM_000988.3 , NM_000988.4 , NM_001349922.1 , NM_001349922.2
Accession Version	NM_001349922.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562601600000
Organism	Homo sapiens(human)
Product	60S ribosomal protein L27
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC055866.19, AW404176.1, CN302602.1 and BC098560.1. On Jun 2, 2019 this sequence version replaced NM_001349922.1. Transcript Variant: This variant (3) retains an intron in its 5' UTR compared to variant 1. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.152870.1, BX400174.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGGCAAGT TCATGAAACC TGGGAAGGTG GTGCTTGTCC TGGCTGGACG CTACTCCGGA 
CGCAAAGCTG TCATCGTGAA GAACATTGAT GATGGCACCT CAGATCGCCC CTACAGCCAT 
GCTCTGGTGG CTGGAATTGA CCGCTACCCC CGCAAAGTGA CAGCTGCCAT GGGCAAGAAG 
AAGATCGCCA AGAGATCAAA GATAAAATCT TTTGTGAAAG TGTATAACTA CAATCACCTA 
ATGCCCACAA GGTACTCTGT GGATATCCCC TTGGACAAAA CTGTCGTCAA TAAGGATGTC 
TTCAGAGATC CTGCTCTTAA ACGCAAGGCC CGACGGGAGG CCAAGGTCAA GTTTGAAGAG 
AGATACAAGA CAGGCAAGAA CAAGTGGTTC TTCCAGAAAC TGCGGTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336851.1
CDS	333..743
Translation	MGKFMKPGKVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF

Target ORF information:

RefSeq Version	NM_001349922.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L27 (RPL27), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001349922.2

ORF Insert Sequence:

ATGGGCAAGT TCATGAAACC TGGGAAGGTG GTGCTTGTCC TGGCTGGACG CTACTCCGGA 
CGCAAAGCTG TCATCGTGAA GAACATTGAT GATGGCACCT CAGATCGCCC CTACAGCCAT 
GCTCTGGTGG CTGGAATTGA CCGCTACCCC CGCAAAGTGA CAGCTGCCAT GGGCAAGAAG 
AAGATCGCCA AGAGATCAAA GATAAAATCT TTTGTGAAAG TGTATAACTA CAATCACCTA 
ATGCCCACAA GGTACTCTGT GGATATCCCC TTGGACAAAA CTGTCGTCAA TAAGGATGTC 
TTCAGAGATC CTGCTCTTAA ACGCAAGGCC CGACGGGAGG CCAAGGTCAA GTTTGAAGAG 
AGATACAAGA CAGGCAAGAA CAAGTGGTTC TTCCAGAAAC TGCGGTTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The DHX33 RNA Helicase Promotes mRNA Translation Initiation.
Molecular and cellular biology35(17)2918-31(2015 Sep)
Zhang Y,You J,Wang X,Weber J

Loss of function mutations in RPL27 and RPS27 identified by whole-exome sequencing in Diamond-Blackfan anaemia.
British journal of haematology168(6)854-64(2015 Mar)
Wang R,Yoshida K,Toki T,Sawada T,Uechi T,Okuno Y,Sato-Otsubo A,Kudo K,Kamimaki I,Kanezaki R,Shiraishi Y,Chiba K,Tanaka H,Terui K,Sato T,Iribe Y,Ohga S,Kuramitsu M,Hamaguchi I,Ohara A,Hara J,Goi K,Matsubara K,Koike K,Ishiguro A,Okamoto Y,Watanabe K,Kanno H,Kojima S,Miyano S,Kenmochi N,Ogawa S,Ito E

SILAC-based proteomics of human primary endothelial cell morphogenesis unveils tumor angiogenic markers.
Molecular & cellular proteomics : MCP12(12)3599-611(2013 Dec)
Zanivan S,Maione F,Hein MY,Hernández-Fernaud JR,Ostasiewicz P,Giraudo E,Mann M

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanhäusser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

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RPL27 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

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ORF Insert Sequence:

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Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

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