RRAGD cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RRAGD
Entrez Gene ID	58528
Full Name	Ras related GTP binding D
Synonyms	RAGD,bA11D8.2.1
General protein information	Preferred Names Ras related GTP binding D Names ras-related GTP-binding protein D Rag D protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6q15
Summary	RRAGD is a monomeric guanine nucleotide-binding protein, or G protein. By binding GTP or GDP, small G proteins act as molecular switches in numerous cell processes and signaling pathways.[supplied by OMIM, Apr 2004].
Disorder MIM:	608268

mRNA and Protein(s)

mRNA	Protein	Name
XM_005248755.4	XP_005248812.1	ras-related GTP-binding protein D isoform X2
XM_005248755.5	XP_005248812.1	ras-related GTP-binding protein D isoform X2
XM_011536022.2	XP_011534324.1	ras-related GTP-binding protein D isoform X1
NM_021244.4	NP_067067.1	ras-related GTP-binding protein D
NM_021244.5	NP_067067.1	ras-related GTP-binding protein D

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	rragd	NP_001072896.1
Macaca mulatta (Rhesus monkey)	LOC702798	XP_001091094.1
Bos taurus (cattle)	RRAGD	NP_001179757.1
Mus musculus (house mouse)	Rragd	NP_081767.2
Rattus norvegicus (Norway rat)	Rragd	NP_001100111.1
Danio rerio (zebrafish)	rragd	NP_001092894.1
Homo sapiens (human)	RRAGD	NP_067067.1
Pan troglodytes (chimpanzee)	RRAGD	XP_001158292.1
Canis lupus familiaris (dog)	RRAGD	XP_532231.4

Gene Ontology
Bibliography

Related articles in PubMed

Control of leucine-dependent mTORC1 pathway through chemical intervention of leucyl-tRNA synthetase and RagD interaction.
Kim JH, Lee C, Lee M, Wang H, Kim K, Park SJ, Yoon I, Jang J, Zhao H, Kim HK, Kwon NH, Jeong SJ, Yoo HC, Kim JH, Yang JS, Lee MY, Lee CW, Yun J, Oh SJ, Kang JS, Martinis SA, Hwang KY, Guo M, Han G, Han JM, Kim S
Nature communications8(1)732(2017 09)

Transcriptional activation of RagD GTPase controls mTORC1 and promotes cancer growth.
Di Malta C, Siciliano D, Calcagni A, Monfregola J, Punzi S, Pastore N, Eastes AN, Davis O, De Cegli R, Zampelli A, Di Giovannantonio LG, Nusco E, Platt N, Guida A, Ogmundsdottir MH, Lanfrancone L, Perera RM, Zoncu R, Pelicci PG, Settembre C, Ballabio A
Science (New York, N.Y.)356(6343)1188-1192(2017 06)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

SLC38A9 is a component of the lysosomal amino acid sensing machinery that controls mTORC1.
Rebsamen M, Pochini L, Stasyk T, de Araújo ME, Galluccio M, Kandasamy RK, Snijder B, Fauster A, Rudashevskaya EL, Bruckner M, Scorzoni S, Filipek PA, Huber KV, Bigenzahn JW, Heinz LX, Kraft C, Bennett KL, Indiveri C, Huber LA, Superti-Furga G
Nature519(7544)477-81(2015 Mar)

Sestrins function as guanine nucleotide dissociation inhibitors for Rag GTPases to control mTORC1 signaling.
Peng M, Yin N, Li MO
Cell159(1)122-133(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RRAGD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RRAGD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu24476	NM_021244.5 Latest version!	Homo sapiens Ras related GTP binding D (RRAGD), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu40922	XM_005248755.5 Latest version!	Homo sapiens Ras related GTP binding D (RRAGD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu71012	XM_011536022.2 Latest version!	Homo sapiens Ras related GTP binding D (RRAGD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu40922	XM_005248755.4	Homo sapiens Ras related GTP binding D (RRAGD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu24476	NM_021244.4	Homo sapiens Ras related GTP binding D (RRAGD), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RRAGD ( NM_021244.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_067067.1 Homo sapiens Ras related GTP binding D (RRAGD), mRNA.

Price & Availability↑

CloneID	OHu24476
Clone ID Related Accession (Same CDS sequence)	NM_021244.4 , NM_021244.5
Accession Version	NM_021244.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1529683200000
Organism	Homo sapiens(human)
Product	ras-related GTP-binding protein D
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL523999.3, DB472739.1, AK289799.1 and AL138717.6. On Nov 29, 2008 this sequence version replaced NM_021244.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.105293.1, SRR1803614.84282.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGAGCCAGG TGCTGGGGAA GCCGCAGCCG CAGGACGAGG ACGACGCGGA GGAGGAGGAG 
GAGGAGGATG AGCTGGTGGG GCTAGCGGAC TACGGAGACG GGCCCGACTC CTCCGACGCC 
GATCCGGACA GCGGCACAGA GGAGGGAGTT CTGGACTTCA GTGACCCCTT CAGCACTGAA 
GTGAAGCCGA GAATCCTGCT CATGGGCCTG AGGAGAAGCG GCAAGTCGTC TATTCAGAAA 
GTTGTCTTTC ACAAAATGTC TCCCAACGAA ACTCTGTTCT TGGAGAGCAC TAATAAGATA 
TGCCGGGAAG ATGTTTCCAA CAGCTCCTTT GTCAATTTTC AGATTTGGGA CTTCCCAGGA 
CAGATTGACT TTTTTGACCC TACATTTGAC TATGAGATGA TCTTCCGGGG AACAGGAGCA 
CTGATATTTG TCATTGACTC ACAGGATGAT TACATGGAAG CCCTGGCCAG GCTCCACCTC 
ACGGTGACCA GGGCCTACAA AGTGAATACT GACATCAACT TCGAGGTGTT TATTCATAAA 
GTGGATGGTC TGTCAGATGA CCACAAAATT GAAACCCAAA GAGATATTCA CCAGAGGGCA 
AACGATGACC TTGCAGATGC TGGATTAGAA AAAATTCACC TCAGCTTTTA TCTGACAAGC 
ATATATGATC ATTCAATATT TGAAGCTTTT AGCAAAGTTG TTCAGAAACT GATTCCACAA 
CTCCCAACTC TGGAGAATTT GCTGAACATC TTTATCTCAA ATTCTGGAAT TGAAAAGGCA 
TTTCTATTTG ATGTGGTCAG TAAAATTTAT ATTGCAACTG ATAGTACTCC GGTGGATATG 
CAAACCTATG AGCTCTGCTG TGATATGATA GATGTGGTTA TTGACATCTC TTGTATTTAT 
GGTCTCAAAG AAGATGGAGC AGGAACCCCC TATGACAAGG AATCCACAGC CATCATAAAG 
CTTAATAATA CAACCGTGCT TTATTTAAAA GAGGTGACAA AGTTCCTGGC TCTCGTTTGC 
TTTGTCAGAG AGGAAAGCTT TGAAAGAAAA GGGCTAATTG ACTATAATTT TCATTGCTTC 
CGGAAGGCCA TTCATGAAGT TTTTGAGGTG AGAATGAAAG TAGTAAAATC TCGAAAGGTT 
CAGAATCGGC TGCAGAAGAA AAAGAGAGCC ACCCCTAATG GGACCCCTAG AGTGCTGCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067067.1
CDS	284..1486
Translation	MSQVLGKPQPQDEDDAEEEEEEDELVGLADYGDGPDSSDADPDSGTEEGVLDFSDPFSTEVKPRILLMGLRRSGKSSIQKVVFHKMSPNETLFLESTNKICREDVSNSSFVNFQIWDFPGQIDFFDPTFDYEMIFRGTGALIFVIDSQDDYMEALARLHLTVTRAYKVNTDINFEVFIHKVDGLSDDHKIETQRDIHQRANDDLADAGLEKIHLSFYLTSIYDHSIFEAFSKVVQKLIPQLPTLENLLNIFISNSGIEKAFLFDVVSKIYIATDSTPVDMQTYELCCDMIDVVIDISCIYGLKEDGAGTPYDKESTAIIKLNNTTVLYLKEVTKFLALVCFVREESFERKGLIDYNFHCFRKAIHEVFEVRMKVVKSRKVQNRLQKKKRATPNGTPRVLL

Target ORF information:

RefSeq Version	NM_021244.4
Organism	Homo sapiens(human)
Definition	Homo sapiens Ras related GTP binding D (RRAGD), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021244.4

ORF Insert Sequence:

ATGAGCCAGG TGCTGGGGAA GCCGCAGCCG CAGGACGAGG ACGACGCGGA GGAGGAGGAG 
GAGGAGGATG AGCTGGTGGG GCTAGCGGAC TACGGAGACG GGCCCGACTC CTCCGACGCC 
GATCCGGACA GCGGCACAGA GGAGGGAGTT CTGGACTTCA GTGACCCCTT CAGCACTGAA 
GTGAAGCCGA GAATCCTGCT CATGGGCCTG AGGAGAAGCG GCAAGTCGTC TATTCAGAAA 
GTTGTCTTTC ACAAAATGTC TCCCAACGAA ACTCTGTTCT TGGAGAGCAC TAATAAGATA 
TGCCGGGAAG ATGTTTCCAA CAGCTCCTTT GTCAATTTTC AGATTTGGGA CTTCCCAGGA 
CAGATTGACT TTTTTGACCC TACATTTGAC TATGAGATGA TCTTCCGGGG AACAGGAGCA 
CTGATATTTG TCATTGACTC ACAGGATGAT TACATGGAAG CCCTGGCCAG GCTCCACCTC 
ACGGTGACCA GGGCCTACAA AGTGAATACT GACATCAACT TCGAGGTGTT TATTCATAAA 
GTGGATGGTC TGTCAGATGA CCACAAAATT GAAACCCAAA GAGATATTCA CCAGAGGGCA 
AACGATGACC TTGCAGATGC TGGATTAGAA AAAATTCACC TCAGCTTTTA TCTGACAAGC 
ATATATGATC ATTCAATATT TGAAGCTTTT AGCAAAGTTG TTCAGAAACT GATTCCACAA 
CTCCCAACTC TGGAGAATTT GCTGAACATC TTTATCTCAA ATTCTGGAAT TGAAAAGGCA 
TTTCTATTTG ATGTGGTCAG TAAAATTTAT ATTGCAACTG ATAGTACTCC GGTGGATATG 
CAAACCTATG AGCTCTGCTG TGATATGATA GATGTGGTTA TTGACATCTC TTGTATTTAT 
GGTCTCAAAG AAGATGGAGC AGGAACCCCC TATGACAAGG AATCCACAGC CATCATAAAG 
CTTAATAATA CAACCGTGCT TTATTTAAAA GAGGTGACAA AGTTCCTGGC TCTCGTTTGC 
TTTGTCAGAG AGGAAAGCTT TGAAAGAAAA GGGCTAATTG ACTATAATTT TCATTGCTTC 
CGGAAGGCCA TTCATGAAGT TTTTGAGGTG AGAATGAAAG TAGTAAAATC TCGAAAGGTT 
CAGAATCGGC TGCAGAAGAA AAAGAGAGCC ACCCCTAATG GGACCCCTAG AGTGCTGCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RRAGD ( NM_021244.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_067067.1 Homo sapiens Ras related GTP binding D (RRAGD), mRNA.

Price & Availability↑

CloneID	OHu24476
Clone ID Related Accession (Same CDS sequence)	NM_021244.4 , NM_021244.5
Accession Version	NM_021244.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570636800000
Organism	Homo sapiens(human)
Product	ras-related GTP-binding protein D
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL523999.3, DB472739.1, AK289799.1 and AL138717.6. On May 17, 2019 this sequence version replaced NM_021244.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.105293.1, SRR1803614.84282.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000369415.9/ ENSP00000358423.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGAGCCAGG TGCTGGGGAA GCCGCAGCCG CAGGACGAGG ACGACGCGGA GGAGGAGGAG 
GAGGAGGATG AGCTGGTGGG GCTAGCGGAC TACGGAGACG GGCCCGACTC CTCCGACGCC 
GATCCGGACA GCGGCACAGA GGAGGGAGTT CTGGACTTCA GTGACCCCTT CAGCACTGAA 
GTGAAGCCGA GAATCCTGCT CATGGGCCTG AGGAGAAGCG GCAAGTCGTC TATTCAGAAA 
GTTGTCTTTC ACAAAATGTC TCCCAACGAA ACTCTGTTCT TGGAGAGCAC TAATAAGATA 
TGCCGGGAAG ATGTTTCCAA CAGCTCCTTT GTCAATTTTC AGATTTGGGA CTTCCCAGGA 
CAGATTGACT TTTTTGACCC TACATTTGAC TATGAGATGA TCTTCCGGGG AACAGGAGCA 
CTGATATTTG TCATTGACTC ACAGGATGAT TACATGGAAG CCCTGGCCAG GCTCCACCTC 
ACGGTGACCA GGGCCTACAA AGTGAATACT GACATCAACT TCGAGGTGTT TATTCATAAA 
GTGGATGGTC TGTCAGATGA CCACAAAATT GAAACCCAAA GAGATATTCA CCAGAGGGCA 
AACGATGACC TTGCAGATGC TGGATTAGAA AAAATTCACC TCAGCTTTTA TCTGACAAGC 
ATATATGATC ATTCAATATT TGAAGCTTTT AGCAAAGTTG TTCAGAAACT GATTCCACAA 
CTCCCAACTC TGGAGAATTT GCTGAACATC TTTATCTCAA ATTCTGGAAT TGAAAAGGCA 
TTTCTATTTG ATGTGGTCAG TAAAATTTAT ATTGCAACTG ATAGTACTCC GGTGGATATG 
CAAACCTATG AGCTCTGCTG TGATATGATA GATGTGGTTA TTGACATCTC TTGTATTTAT 
GGTCTCAAAG AAGATGGAGC AGGAACCCCC TATGACAAGG AATCCACAGC CATCATAAAG 
CTTAATAATA CAACCGTGCT TTATTTAAAA GAGGTGACAA AGTTCCTGGC TCTCGTTTGC 
TTTGTCAGAG AGGAAAGCTT TGAAAGAAAA GGGCTAATTG ACTATAATTT TCATTGCTTC 
CGGAAGGCCA TTCATGAAGT TTTTGAGGTG AGAATGAAAG TAGTAAAATC TCGAAAGGTT 
CAGAATCGGC TGCAGAAGAA AAAGAGAGCC ACCCCTAATG GGACCCCTAG AGTGCTGCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067067.1
CDS	281..1483
Translation	MSQVLGKPQPQDEDDAEEEEEEDELVGLADYGDGPDSSDADPDSGTEEGVLDFSDPFSTEVKPRILLMGLRRSGKSSIQKVVFHKMSPNETLFLESTNKICREDVSNSSFVNFQIWDFPGQIDFFDPTFDYEMIFRGTGALIFVIDSQDDYMEALARLHLTVTRAYKVNTDINFEVFIHKVDGLSDDHKIETQRDIHQRANDDLADAGLEKIHLSFYLTSIYDHSIFEAFSKVVQKLIPQLPTLENLLNIFISNSGIEKAFLFDVVSKIYIATDSTPVDMQTYELCCDMIDVVIDISCIYGLKEDGAGTPYDKESTAIIKLNNTTVLYLKEVTKFLALVCFVREESFERKGLIDYNFHCFRKAIHEVFEVRMKVVKSRKVQNRLQKKKRATPNGTPRVLL

Target ORF information:

RefSeq Version	NM_021244.5
Organism	Homo sapiens(human)
Definition	Homo sapiens Ras related GTP binding D (RRAGD), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021244.5

ORF Insert Sequence:

ATGAGCCAGG TGCTGGGGAA GCCGCAGCCG CAGGACGAGG ACGACGCGGA GGAGGAGGAG 
GAGGAGGATG AGCTGGTGGG GCTAGCGGAC TACGGAGACG GGCCCGACTC CTCCGACGCC 
GATCCGGACA GCGGCACAGA GGAGGGAGTT CTGGACTTCA GTGACCCCTT CAGCACTGAA 
GTGAAGCCGA GAATCCTGCT CATGGGCCTG AGGAGAAGCG GCAAGTCGTC TATTCAGAAA 
GTTGTCTTTC ACAAAATGTC TCCCAACGAA ACTCTGTTCT TGGAGAGCAC TAATAAGATA 
TGCCGGGAAG ATGTTTCCAA CAGCTCCTTT GTCAATTTTC AGATTTGGGA CTTCCCAGGA 
CAGATTGACT TTTTTGACCC TACATTTGAC TATGAGATGA TCTTCCGGGG AACAGGAGCA 
CTGATATTTG TCATTGACTC ACAGGATGAT TACATGGAAG CCCTGGCCAG GCTCCACCTC 
ACGGTGACCA GGGCCTACAA AGTGAATACT GACATCAACT TCGAGGTGTT TATTCATAAA 
GTGGATGGTC TGTCAGATGA CCACAAAATT GAAACCCAAA GAGATATTCA CCAGAGGGCA 
AACGATGACC TTGCAGATGC TGGATTAGAA AAAATTCACC TCAGCTTTTA TCTGACAAGC 
ATATATGATC ATTCAATATT TGAAGCTTTT AGCAAAGTTG TTCAGAAACT GATTCCACAA 
CTCCCAACTC TGGAGAATTT GCTGAACATC TTTATCTCAA ATTCTGGAAT TGAAAAGGCA 
TTTCTATTTG ATGTGGTCAG TAAAATTTAT ATTGCAACTG ATAGTACTCC GGTGGATATG 
CAAACCTATG AGCTCTGCTG TGATATGATA GATGTGGTTA TTGACATCTC TTGTATTTAT 
GGTCTCAAAG AAGATGGAGC AGGAACCCCC TATGACAAGG AATCCACAGC CATCATAAAG 
CTTAATAATA CAACCGTGCT TTATTTAAAA GAGGTGACAA AGTTCCTGGC TCTCGTTTGC 
TTTGTCAGAG AGGAAAGCTT TGAAAGAAAA GGGCTAATTG ACTATAATTT TCATTGCTTC 
CGGAAGGCCA TTCATGAAGT TTTTGAGGTG AGAATGAAAG TAGTAAAATC TCGAAAGGTT 
CAGAATCGGC TGCAGAAGAA AAAGAGAGCC ACCCCTAATG GGACCCCTAG AGTGCTGCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RRAGD ( XM_005248755.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248812.1 Homo sapiens Ras related GTP binding D (RRAGD), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu40922
Clone ID Related Accession (Same CDS sequence)	XM_005248755.4 , XM_005248755.5
Accession Version	XM_005248755.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	ras-related GTP-binding protein D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005248755.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAGG TGCTGGGGAA GCCGCAGCCG CAGGACGAGG ACGACGCGGA GGAGGAGGAG 
GAGGAGGATG AGCTGGTGGG GCTAGCGGAC TACGGAGACG GGCCCGACTC CTCCGACGCC 
GATCCGGACA GCGGCACAGA GGAGGGAGTT CTGGACTTCA GTGACCCCTT CAGCACTGAA 
GTGAAGCCGA GAATCCTGCT CATGGGCCTG AGGAGAAGCG GCAAGTCGTC TATTCAGAAA 
GTTGTCTTTC ACAAAATGTC TCCCAACGAA ACTCTGTTCT TGGAGAGCAC TAATAAGATA 
TGCCGGGAAG ATGTTTCCAA CAGCTCCTTT GTCAATTTTC AGATTTGGGA CTTCCCAGGA 
CAGATTGACT TTTTTGACCC TACATTTGAC TATGAGATGA TCTTCCGGGG AACAGGAGCA 
CTGATATTTG TCATTGACTC ACAGGATGAT TACATGGAAG CCCTGGCCAG GCTCCACCTC 
ACGGTGACCA GGGCCTACAA AGTGAATACT GACATCAACT TCGAGGTGTT TATTCATAAA 
GTGGATGGTC TGTCAGATGA CCACAAAATT GAAACCCAAA GAGATATTCA CCAGAGGGCA 
AACGATGACC TTGCAGATGC TGGATTAGAA AAAATTCACC TCAGCTTTTA TCTGACAAGC 
ATATATGATC ATTCAATATT TGAAGCTTTT AGCAAAGTTG TTCAGAAACT GATTCCACAA 
CTCCCAACTC TGGAGAATTT GCTGAACATC TTTATCTCAA ATTCTGGAAT TGAAAAGGCA 
TTTCTATTTG ATGTGGTCAG TAAAATTTAT ATTGCAACTG ATAGTACTCC GGTGGATATG 
CAAACCTATG AGCTCTGCTG TGATATGATA GATGTGGTTA TTGACATCTC TTGTATTTAT 
GGGGCTAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248812.1
CDS	478..1389
Translation	MSQVLGKPQPQDEDDAEEEEEEDELVGLADYGDGPDSSDADPDSGTEEGVLDFSDPFSTEVKPRILLMGLRRSGKSSIQKVVFHKMSPNETLFLESTNKICREDVSNSSFVNFQIWDFPGQIDFFDPTFDYEMIFRGTGALIFVIDSQDDYMEALARLHLTVTRAYKVNTDINFEVFIHKVDGLSDDHKIETQRDIHQRANDDLADAGLEKIHLSFYLTSIYDHSIFEAFSKVVQKLIPQLPTLENLLNIFISNSGIEKAFLFDVVSKIYIATDSTPVDMQTYELCCDMIDVVIDISCIYGAN

Target ORF information:

RefSeq Version	XM_005248755.4
Organism	Homo sapiens(human)
Definition	Homo sapiens Ras related GTP binding D (RRAGD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248755.4

ORF Insert Sequence:

ATGAGCCAGG TGCTGGGGAA GCCGCAGCCG CAGGACGAGG ACGACGCGGA GGAGGAGGAG 
GAGGAGGATG AGCTGGTGGG GCTAGCGGAC TACGGAGACG GGCCCGACTC CTCCGACGCC 
GATCCGGACA GCGGCACAGA GGAGGGAGTT CTGGACTTCA GTGACCCCTT CAGCACTGAA 
GTGAAGCCGA GAATCCTGCT CATGGGCCTG AGGAGAAGCG GCAAGTCGTC TATTCAGAAA 
GTTGTCTTTC ACAAAATGTC TCCCAACGAA ACTCTGTTCT TGGAGAGCAC TAATAAGATA 
TGCCGGGAAG ATGTTTCCAA CAGCTCCTTT GTCAATTTTC AGATTTGGGA CTTCCCAGGA 
CAGATTGACT TTTTTGACCC TACATTTGAC TATGAGATGA TCTTCCGGGG AACAGGAGCA 
CTGATATTTG TCATTGACTC ACAGGATGAT TACATGGAAG CCCTGGCCAG GCTCCACCTC 
ACGGTGACCA GGGCCTACAA AGTGAATACT GACATCAACT TCGAGGTGTT TATTCATAAA 
GTGGATGGTC TGTCAGATGA CCACAAAATT GAAACCCAAA GAGATATTCA CCAGAGGGCA 
AACGATGACC TTGCAGATGC TGGATTAGAA AAAATTCACC TCAGCTTTTA TCTGACAAGC 
ATATATGATC ATTCAATATT TGAAGCTTTT AGCAAAGTTG TTCAGAAACT GATTCCACAA 
CTCCCAACTC TGGAGAATTT GCTGAACATC TTTATCTCAA ATTCTGGAAT TGAAAAGGCA 
TTTCTATTTG ATGTGGTCAG TAAAATTTAT ATTGCAACTG ATAGTACTCC GGTGGATATG 
CAAACCTATG AGCTCTGCTG TGATATGATA GATGTGGTTA TTGACATCTC TTGTATTTAT 
GGGGCTAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RRAGD ( XM_005248755.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248812.1 Homo sapiens Ras related GTP binding D (RRAGD), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu40922
Clone ID Related Accession (Same CDS sequence)	XM_005248755.4 , XM_005248755.5
Accession Version	XM_005248755.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	ras-related GTP-binding protein D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005248755.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAGG TGCTGGGGAA GCCGCAGCCG CAGGACGAGG ACGACGCGGA GGAGGAGGAG 
GAGGAGGATG AGCTGGTGGG GCTAGCGGAC TACGGAGACG GGCCCGACTC CTCCGACGCC 
GATCCGGACA GCGGCACAGA GGAGGGAGTT CTGGACTTCA GTGACCCCTT CAGCACTGAA 
GTGAAGCCGA GAATCCTGCT CATGGGCCTG AGGAGAAGCG GCAAGTCGTC TATTCAGAAA 
GTTGTCTTTC ACAAAATGTC TCCCAACGAA ACTCTGTTCT TGGAGAGCAC TAATAAGATA 
TGCCGGGAAG ATGTTTCCAA CAGCTCCTTT GTCAATTTTC AGATTTGGGA CTTCCCAGGA 
CAGATTGACT TTTTTGACCC TACATTTGAC TATGAGATGA TCTTCCGGGG AACAGGAGCA 
CTGATATTTG TCATTGACTC ACAGGATGAT TACATGGAAG CCCTGGCCAG GCTCCACCTC 
ACGGTGACCA GGGCCTACAA AGTGAATACT GACATCAACT TCGAGGTGTT TATTCATAAA 
GTGGATGGTC TGTCAGATGA CCACAAAATT GAAACCCAAA GAGATATTCA CCAGAGGGCA 
AACGATGACC TTGCAGATGC TGGATTAGAA AAAATTCACC TCAGCTTTTA TCTGACAAGC 
ATATATGATC ATTCAATATT TGAAGCTTTT AGCAAAGTTG TTCAGAAACT GATTCCACAA 
CTCCCAACTC TGGAGAATTT GCTGAACATC TTTATCTCAA ATTCTGGAAT TGAAAAGGCA 
TTTCTATTTG ATGTGGTCAG TAAAATTTAT ATTGCAACTG ATAGTACTCC GGTGGATATG 
CAAACCTATG AGCTCTGCTG TGATATGATA GATGTGGTTA TTGACATCTC TTGTATTTAT 
GGGGCTAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248812.1
CDS	209..1120
Translation	MSQVLGKPQPQDEDDAEEEEEEDELVGLADYGDGPDSSDADPDSGTEEGVLDFSDPFSTEVKPRILLMGLRRSGKSSIQKVVFHKMSPNETLFLESTNKICREDVSNSSFVNFQIWDFPGQIDFFDPTFDYEMIFRGTGALIFVIDSQDDYMEALARLHLTVTRAYKVNTDINFEVFIHKVDGLSDDHKIETQRDIHQRANDDLADAGLEKIHLSFYLTSIYDHSIFEAFSKVVQKLIPQLPTLENLLNIFISNSGIEKAFLFDVVSKIYIATDSTPVDMQTYELCCDMIDVVIDISCIYGAN

Target ORF information:

RefSeq Version	XM_005248755.5
Organism	Homo sapiens(human)
Definition	Homo sapiens Ras related GTP binding D (RRAGD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248755.5

ORF Insert Sequence:

ATGAGCCAGG TGCTGGGGAA GCCGCAGCCG CAGGACGAGG ACGACGCGGA GGAGGAGGAG 
GAGGAGGATG AGCTGGTGGG GCTAGCGGAC TACGGAGACG GGCCCGACTC CTCCGACGCC 
GATCCGGACA GCGGCACAGA GGAGGGAGTT CTGGACTTCA GTGACCCCTT CAGCACTGAA 
GTGAAGCCGA GAATCCTGCT CATGGGCCTG AGGAGAAGCG GCAAGTCGTC TATTCAGAAA 
GTTGTCTTTC ACAAAATGTC TCCCAACGAA ACTCTGTTCT TGGAGAGCAC TAATAAGATA 
TGCCGGGAAG ATGTTTCCAA CAGCTCCTTT GTCAATTTTC AGATTTGGGA CTTCCCAGGA 
CAGATTGACT TTTTTGACCC TACATTTGAC TATGAGATGA TCTTCCGGGG AACAGGAGCA 
CTGATATTTG TCATTGACTC ACAGGATGAT TACATGGAAG CCCTGGCCAG GCTCCACCTC 
ACGGTGACCA GGGCCTACAA AGTGAATACT GACATCAACT TCGAGGTGTT TATTCATAAA 
GTGGATGGTC TGTCAGATGA CCACAAAATT GAAACCCAAA GAGATATTCA CCAGAGGGCA 
AACGATGACC TTGCAGATGC TGGATTAGAA AAAATTCACC TCAGCTTTTA TCTGACAAGC 
ATATATGATC ATTCAATATT TGAAGCTTTT AGCAAAGTTG TTCAGAAACT GATTCCACAA 
CTCCCAACTC TGGAGAATTT GCTGAACATC TTTATCTCAA ATTCTGGAAT TGAAAAGGCA 
TTTCTATTTG ATGTGGTCAG TAAAATTTAT ATTGCAACTG ATAGTACTCC GGTGGATATG 
CAAACCTATG AGCTCTGCTG TGATATGATA GATGTGGTTA TTGACATCTC TTGTATTTAT 
GGGGCTAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RRAGD ( XM_011536022.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534324.1 Homo sapiens Ras related GTP binding D (RRAGD), transcript variant X1, mRNA.

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CloneID	OHu71012
Clone ID Related Accession (Same CDS sequence)	XM_011536022.2
Accession Version	XM_011536022.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	ras-related GTP-binding protein D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011536022.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTGGC TAAAAAAAGG CAGCCGGCGG CGGCGTGACT GGCAGGGTCG TCGGCAGCTG 
CGGGTGTCAG TCTCGCCCCG CGTCGCCGCG GGAGGGGCCC ACCCAGTTCT GGACTTCAGT 
GACCCCTTCA GCACTGAAGT GAAGCCGAGA ATCCTGCTCA TGGGCCTGAG GAGAAGCGGC 
AAGTCGTCTA TTCAGAAAGT TGTCTTTCAC AAAATGTCTC CCAACGAAAC TCTGTTCTTG 
GAGAGCACTA ATAAGATATG CCGGGAAGAT GTTTCCAACA GCTCCTTTGT CAATTTTCAG 
ATTTGGGACT TCCCAGGACA GATTGACTTT TTTGACCCTA CATTTGACTA TGAGATGATC 
TTCCGGGGAA CAGGAGCACT GATATTTGTC ATTGACTCAC AGGATGATTA CATGGAAGCC 
CTGGCCAGGC TCCACCTCAC GGTGACCAGG GCCTACAAAG TGAATACTGA CATCAACTTC 
GAGGTGTTTA TTCATAAAGT GGATGGTCTG TCAGATGACC ACAAAATTGA AACCCAAAGA 
GATATTCACC AGAGGGCAAA CGATGACCTT GCAGATGCTG GATTAGAAAA AATTCACCTC 
AGCTTTTATC TGACAAGCAT ATATGATCAT TCAATATTTG AAGCTTTTAG CAAAGTTGTT 
CAGAAACTGA TTCCACAACT CCCAACTCTG GAGAATTTGC TGAACATCTT TATCTCAAAT 
TCTGGAATTG AAAAGGCATT TCTATTTGAT GTGGTCAGTA AAATTTATAT TGCAACTGAT 
AGTACTCCGG TGGATATGCA AACCTATGAG CTCTGCTGTG ATATGATAGA TGTGGTTATT 
GACATCTCTT GTATTTATGG TCTCAAAGAA GATGGAGCAG GAACCCCCTA TGACAAGGAA 
TCCACAGCCA TCATAAAGCT TAATAATACA ACCGTGCTTT ATTTAAAAGA GGTGACAAAG 
TTCCTGGCTC TCGTTTGCTT TGTCAGAGAG GAAAGCTTTG AAAGAAAAGG GCTAATTGAC 
TATAATTTTC ATTGCTTCCG GAAGGCCATT CATGAAGTTT TTGAGGTGAG AATGAAAGTA 
GTAAAATCTC GAAAGGTTCA GAATCGGCTG CAGAAGAAAA AGAGAGCCAC CCCTAATGGG 
ACCCCTAGAG TGCTGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534324.1
CDS	98..1258
Translation	MSWLKKGSRRRRDWQGRRQLRVSVSPRVAAGGAHPVLDFSDPFSTEVKPRILLMGLRRSGKSSIQKVVFHKMSPNETLFLESTNKICREDVSNSSFVNFQIWDFPGQIDFFDPTFDYEMIFRGTGALIFVIDSQDDYMEALARLHLTVTRAYKVNTDINFEVFIHKVDGLSDDHKIETQRDIHQRANDDLADAGLEKIHLSFYLTSIYDHSIFEAFSKVVQKLIPQLPTLENLLNIFISNSGIEKAFLFDVVSKIYIATDSTPVDMQTYELCCDMIDVVIDISCIYGLKEDGAGTPYDKESTAIIKLNNTTVLYLKEVTKFLALVCFVREESFERKGLIDYNFHCFRKAIHEVFEVRMKVVKSRKVQNRLQKKKRATPNGTPRVLL

Target ORF information:

RefSeq Version	XM_011536022.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Ras related GTP binding D (RRAGD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536022.2

ORF Insert Sequence:

ATGTCGTGGC TAAAAAAAGG CAGCCGGCGG CGGCGTGACT GGCAGGGTCG TCGGCAGCTG 
CGGGTGTCAG TCTCGCCCCG CGTCGCCGCG GGAGGGGCCC ACCCAGTTCT GGACTTCAGT 
GACCCCTTCA GCACTGAAGT GAAGCCGAGA ATCCTGCTCA TGGGCCTGAG GAGAAGCGGC 
AAGTCGTCTA TTCAGAAAGT TGTCTTTCAC AAAATGTCTC CCAACGAAAC TCTGTTCTTG 
GAGAGCACTA ATAAGATATG CCGGGAAGAT GTTTCCAACA GCTCCTTTGT CAATTTTCAG 
ATTTGGGACT TCCCAGGACA GATTGACTTT TTTGACCCTA CATTTGACTA TGAGATGATC 
TTCCGGGGAA CAGGAGCACT GATATTTGTC ATTGACTCAC AGGATGATTA CATGGAAGCC 
CTGGCCAGGC TCCACCTCAC GGTGACCAGG GCCTACAAAG TGAATACTGA CATCAACTTC 
GAGGTGTTTA TTCATAAAGT GGATGGTCTG TCAGATGACC ACAAAATTGA AACCCAAAGA 
GATATTCACC AGAGGGCAAA CGATGACCTT GCAGATGCTG GATTAGAAAA AATTCACCTC 
AGCTTTTATC TGACAAGCAT ATATGATCAT TCAATATTTG AAGCTTTTAG CAAAGTTGTT 
CAGAAACTGA TTCCACAACT CCCAACTCTG GAGAATTTGC TGAACATCTT TATCTCAAAT 
TCTGGAATTG AAAAGGCATT TCTATTTGAT GTGGTCAGTA AAATTTATAT TGCAACTGAT 
AGTACTCCGG TGGATATGCA AACCTATGAG CTCTGCTGTG ATATGATAGA TGTGGTTATT 
GACATCTCTT GTATTTATGG TCTCAAAGAA GATGGAGCAG GAACCCCCTA TGACAAGGAA 
TCCACAGCCA TCATAAAGCT TAATAATACA ACCGTGCTTT ATTTAAAAGA GGTGACAAAG 
TTCCTGGCTC TCGTTTGCTT TGTCAGAGAG GAAAGCTTTG AAAGAAAAGG GCTAATTGAC 
TATAATTTTC ATTGCTTCCG GAAGGCCATT CATGAAGTTT TTGAGGTGAG AATGAAAGTA 
GTAAAATCTC GAAAGGTTCA GAATCGGCTG CAGAAGAAAA AGAGAGCCAC CCCTAATGGG 
ACCCCTAGAG TGCTGCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Control of leucine-dependent mTORC1 pathway through chemical intervention of leucyl-tRNA synthetase and RagD interaction.
Nature communications8(1)732(2017 09)
Kim JH,Lee C,Lee M,Wang H,Kim K,Park SJ,Yoon I,Jang J,Zhao H,Kim HK,Kwon NH,Jeong SJ,Yoo HC,Kim JH,Yang JS,Lee MY,Lee CW,Yun J,Oh SJ,Kang JS,Martinis SA,Hwang KY,Guo M,Han G,Han JM,Kim S

Transcriptional activation of RagD GTPase controls mTORC1 and promotes cancer growth.
Science (New York, N.Y.)356(6343)1188-1192(2017 06)
Di Malta C,Siciliano D,Calcagni A,Monfregola J,Punzi S,Pastore N,Eastes AN,Davis O,De Cegli R,Zampelli A,Di Giovannantonio LG,Nusco E,Platt N,Guida A,Ogmundsdottir MH,Lanfrancone L,Perera RM,Zoncu R,Pelicci PG,Settembre C,Ballabio A

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

SLC38A9 is a component of the lysosomal amino acid sensing machinery that controls mTORC1.
Nature519(7544)477-81(2015 Mar)
Rebsamen M,Pochini L,Stasyk T,de Araújo ME,Galluccio M,Kandasamy RK,Snijder B,Fauster A,Rudashevskaya EL,Bruckner M,Scorzoni S,Filipek PA,Huber KV,Bigenzahn JW,Heinz LX,Kraft C,Bennett KL,Indiveri C,Huber LA,Superti-Furga G

Sestrins function as guanine nucleotide dissociation inhibitors for Rag GTPases to control mTORC1 signaling.
Cell159(1)122-133(2014 Sep)
Peng M,Yin N,Li MO

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RRAGD cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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