SLC12A5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SLC12A5
Entrez Gene ID	57468
Full Name	solute carrier family 12 member 5
Synonyms	EIEE34,EIG14,KCC2,hKCC2
General protein information	Preferred Names solute carrier family 12 member 5 Names solute carrier family 12 member 5 K-Cl cotransporter 2 electroneutral potassium-chloride cotransporter 2 erythroid K-Cl cotransporter 2 neuronal K-Cl cotransporter solute carrier family 12 (potassium/chloride transporter), member 5
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20q13.12
Summary	K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Sep 2008].
Disorder MIM:	606726
Disorder Html:	Epileptic encephalopathy, early infantile, 34, 616645 (3); {Epilepsy, idiopathic generalized, susceptibility to, 14}, 616685 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017027981.1	XP_016883470.1	solute carrier family 12 member 5 isoform X1
NM_020708.4	NP_065759.1	solute carrier family 12 member 5 isoform 2
NM_001134771.1	NP_001128243.1	solute carrier family 12 member 5 isoform 1
NM_020708.5	NP_065759.1	solute carrier family 12 member 5 isoform 2
NM_001134771.2	NP_001128243.1	solute carrier family 12 member 5 isoform 1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	SLC12A5	XP_001236722.3
Macaca mulatta (Rhesus monkey)	SLC12A5	XP_001104798.1
Bos taurus (cattle)	SLC12A5	NP_001193309.1
Pan troglodytes (chimpanzee)	SLC12A5	XP_003317037.1
Rattus norvegicus (Norway rat)	Slc12a5	NP_599190.1
Oryza sativa (rice)	Os08g0323700	NP_001061543.1
Danio rerio (zebrafish)	slc12a5b	XP_003199160.2
Danio rerio (zebrafish)	LOC572215	XP_701000.5
Homo sapiens (human)	SLC12A5	NP_065759.1
Canis lupus familiaris (dog)	SLC12A5	XP_005635409.1
Mus musculus (house mouse)	Slc12a5	NP_065066.2
Arabidopsis thaliana (thale cress)	CCC1	NP_174333.2
Xenopus tropicalis (tropical clawed frog)	slc12a5	NP_001072306.2

Gene Ontology
Bibliography

Related articles in PubMed

Methylation of cation-chloride cotransporters NKCC1 and KCC2 in patients with juvenile myoclonic epilepsy.
Genç F, Kara M, Ünal Y, Uygur Küçükseymen E, Biçer Gömceli Y, Kaynar T, Tosun K, Kutlu G
Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology40(5)1007-1013(2019 May)

Brain microstructural abnormalities correlate with KCC2 downregulation in refractory epilepsy.
Gharaylou Z, Oghabian MA, Azizi Z, Hadjighassem M
Neuroreport30(6)409-414(2019 04)

Phosphoregulation of the intracellular termini of K
Cordshagen A, Busch W, Winklhofer M, Nothwang HG, Hartmann AM
The Journal of biological chemistry293(44)16984-16993(2018 11)

The neuronal K
Kursan S, McMillen TS, Beesetty P, Dias-Junior E, Almutairi MM, Sajib AA, Kozak JA, Aguilar-Bryan L, Di Fulvio M
Scientific reports7(1)1732(2017 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SLC12A5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC12A5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu108526	NM_001134771.2 Latest version!	Homo sapiens solute carrier family 12 member 5 (SLC12A5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu108528	NM_020708.5 Latest version!	Homo sapiens solute carrier family 12 member 5 (SLC12A5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu93091	XM_017027981.1 Latest version!	Homo sapiens solute carrier family 12 member 5 (SLC12A5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu09712	NM_001134771.1 Latest version!	Homo sapiens solute carrier family 12 member 5 (SLC12A5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$594.30 $849.00
OHu10133	NM_020708.4 Latest version!	Homo sapiens solute carrier family 12 member 5 (SLC12A5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SLC12A5 ( NM_001134771.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001128243.1 Homo sapiens solute carrier family 12 member 5 (SLC12A5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu108526
Clone ID Related Accession (Same CDS sequence)	NM_001134771.2
Accession Version	NM_001134771.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577980800000
Organism	Homo sapiens(human)
Product	solute carrier family 12 member 5 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162458.10. On Jun 2, 2019 this sequence version replaced NM_001134771.1. Transcript Variant: This variant (1) encodes the longer isoform (1, also known as KCC2a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCGCA GGTTCACGGT CACCTCGCTG CCCCCCGCAG GGCCCGCCAG AAGCCCTGAC 
CCAGAGTCCC GCCGGCATTC GGTCGCAGAC CCCCGCCACC TCCCGGGGGA AGACGTCAAA 
GGTGATGGCA ACCCCAAGGA AAGCAGTCCC TTCATCAACA GCACCGACAC AGAGAAGGGA 
AAGGAGTATG ATGGCAAGAA CATGGCCTTG TTTGAGGAGG AGATGGACAC CAGCCCTATG 
GTGTCCTCCT TGCTCAGTGG CCTGGCCAAC TACACCAACC TGCCCCAGGG AAGTAGGGAG 
CATGAAGAGG CAGAAAACAA TGAGGGTGGA AAAAAGAAGC CGGTGCAGGC CCCACGCATG 
GGCACCTTCA TGGGCGTGTA CCTGCCGTGC CTGCAGAACA TCTTTGGCGT CATCCTCTTC 
CTGCGGCTCA CCTGGGTGGT GGGCATTGCA GGCATCATGG AGTCCTTCTG CATGGTGTTC 
ATCTGCTGCT CCTGTACGAT GCTCACGGCC ATCTCCATGA GTGCAATTGC AACGAATGGT 
GTTGTGCCTG CTGGTGGCTC CTACTACATG ATTTCCAGGT CTCTGGGCCC AGAGTTTGGG 
GGTGCCGTGG GCCTCTGCTT CTACCTGGGC ACTACCTTTG CAGGAGCCAT GTACATCCTG 
GGCACCATCG AAATCCTGCT GGCTTACCTC TTCCCAGCCA TGGCCATCTT CAAGGCAGAA 
GATGCCAGTG GGGAGGCAGC AGCCATGCTG AACAACATGC GTGTTTACGG CACCTGTGTG 
CTCACCTGCA TGGCCACTGT GGTGTTTGTG GGTGTCAAGT ATGTCAACAA GTTTGCCCTT 
GTCTTCCTGG GTTGTGTCAT CCTCTCCATC CTGGCCATCT ATGCTGGGGT CATCAAGTCT 
GCCTTCGACC CACCCAACTT CCCGATCTGC CTCCTGGGTA ACCGCACGCT GTCTCGCCAT 
GGCTTTGATG TCTGTGCCAA GCTGGCTTGG GAAGGAAATG AGACGGTGAC CACACGGCTA 
TGGGGCCTTT TCTGCTCCTC TCGCTTCCTC AACGCCACCT GTGATGAATA CTTCACCCGA 
AACAATGTCA CAGAGATCCA GGGCATCCCT GGTGCTGCCA GTGGCCTCAT CAAAGAGAAC 
CTCTGGAGCT CCTACCTGAC CAAGGGCGTG ATTGTGGAGA GGAGTGGGAT GACCTCGGTG 
GGCCTGGCCG ATGGCACTCC TATCGACATG GACCACCCTT ATGTCTTCAG TGATATGACC 
TCCTACTTCA CCCTGCTGGT TGGCATCTAC TTCCCCTCAG TCACAGGGAT CATGGCTGGT 
TCTAACCGCT CTGGGGACCT GAGGGATGCC CAGAAGTCAA TCCCCACTGG CACCATCCTG 
GCCATCGCCA CCACCTCTGC TGTCTACATC AGCTCCGTTG TTCTGTTTGG GGCCTGCATT 
GAGGGGGTCG TCCTGCGGGA CAAGTTTGGC GAAGCTGTGA ATGGCAACCT CGTGGTGGGC 
ACTCTGGCCT GGCCATCTCC ATGGGTAATT GTCATCGGAT CCTTCTTCTC CACCTGTGGG 
GCTGGGCTGC AGAGCCTCAC GGGGGCCCCA CGCCTGCTGC AGGCCATCTC GAGGGATGGC 
ATTGTGCCCT TCCTGCAGGT CTTTGGCCAT GGCAAGGCCA ATGGAGAGCC GACCTGGGCC 
CTGCTCCTGA CTGCCTGCAT CTGCGAGATT GGCATCCTCA TTGCATCCCT CGACGAGGTG 
GCCCCCATCC TCTCTATGTT CTTCCTGATG TGCTACATGT TTGTGAATCT GGCCTGTGCA 
GTGCAGACGC TGCTGAGGAC ACCCAACTGG AGGCCACGCT TTCGATATTA CCACTGGACC 
CTCTCCTTCC TGGGCATGAG CCTCTGCCTG GCCCTCATGT TCATCTGCTC CTGGTATTAT 
GCACTGGTAG CCATGCTCAT TGCTGGACTC ATCTACAAGT ACATTGAGTA CCGTGGGGCA 
GAGAAGGAGT GGGGCGATGG GATACGAGGT CTGTCTCTCA GTGCGGCTCG CTATGCCCTC 
TTACGCCTGG AGGAAGGGCC CCCACACACC AAGAACTGGA GGCCACAGCT GCTGGTGCTG 
GTGCGTGTGG ACCAAGACCA GAATGTGGTG CACCCCCAGC TGCTCTCACT GACCTCCCAG 
CTGAAGGCGG GGAAGGGCCT GACCATCGTG GGCTCTGTCC TTGAGGGCAC CTTTCTGGAA 
AATCATCCAC AGGCCCAGCG GGCAGAAGAG TCTATCAGGC GCCTGATGGA GGCAGAGAAG 
GTGAAGGGCT TCTGCCAGGT GGTGATCTCC TCCAACTTGC GTGATGGCGT GTCCCATCTG 
ATCCAGTCCG GGGGCCTCGG GGGGCTGCAG CACAACACTG TGCTTGTTGG CTGGCCCCGC 
AACTGGCGCC AGAAGGAAGA TCATCAGACG TGGAGGAACT TCATTGAGCT GGTCCGGGAA 
ACCACAGCTG GCCACTTAGC CCTGCTGGTC ACCAAGAACG TTTCCATGTT TCCTGGGAAC 
CCTGAGCGCT TCTCTGAGGG CAGCATCGAC GTTTGGTGGA TTGTGCACGA TGGAGGCATG 
CTCATGCTGC TGCCCTTCCT GCTGCGGCAC CACAAGGTCT GGCGGAAGTG CAAGATGCGT 
ATCTTCACTG TGGCCCAGAT GGATGACAAT AGCATCCAGA TGAAGAAGGA TCTGACCACA 
TTTCTGTATC ATTTACGCAT CACTGCGGAG GTCGAGGTGG TGGAGATGCA TGAGAGCGAC 
ATCTCAGCTT ACACCTATGA GAAGACGTTG GTGATGGAGC AGCGTTCCCA GATCCTCAAA 
CAGATGCATT TAACCAAGAA TGAGCGGGAG CGGGAGATCC AGAGTATCAC AGATGAGTCA 
CGAGGCTCAA TCCGGAGAAA GAATCCAGCC AACACGCGGC TCCGCCTGAA CGTCCCAGAA 
GAGACGGCTG GTGACAGTGA AGAGAAGCCA GAGGAGGAGG TGCAGCTGAT CCACGATCAG 
AGTGCTCCCA GCTGCCCCAG CAGCTCCCCG TCCCCAGGGG AGGAGCCTGA GGGGGAAGGG 
GAGACAGATC CGGAGAAGGT GCATCTCACC TGGACCAAGG ACAAGTCGGT GGCAGAGAAG 
AATAAGGGCC CCAGTCCTGT CTCCTCTGAG GGCATCAAGG ACTTCTTCAG CATGAAGCCG 
GAGTGGGAGA ACTTGAACCA GTCCAACGTG CGGCGCATGC ACACGGCCGT GCGGCTGAAC 
GAGGTCATCG TGAAGAAATC CCGGGACGCC AAGCTTGTTT TGCTCAACAT GCCTGGGCCT 
CCCCGCAACC GCAATGGTGA TGAAAACTAC ATGGAGTTTC TCGAGGTCCT CACAGAGCAC 
CTGGACCGGG TGATGCTGGT CCGCGGCGGC GGCCGCGAGG TCATCACCAT CTACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128243.1
CDS	81..3500
Translation	MSRRFTVTSLPPAGPARSPDPESRRHSVADPRHLPGEDVKGDGNPKESSPFINSTDTEKGKEYDGKNMALFEEEMDTSPMVSSLLSGLANYTNLPQGSREHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAEDASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIKSAFDPPNFPICLLGNRTLSRHGFDVCAKLAWEGNETVTTRLWGLFCSSRFLNATCDEYFTRNNVTEIQGIPGAASGLIKENLWSSYLTKGVIVERSGMTSVGLADGTPIDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQDQNVVHPQLLSLTSQLKAGKGLTIVGSVLEGTFLENHPQAQRAEESIRRLMEAEKVKGFCQVVISSNLRDGVSHLIQSGGLGGLQHNTVLVGWPRNWRQKEDHQTWRNFIELVRETTAGHLALLVTKNVSMFPGNPERFSEGSIDVWWIVHDGGMLMLLPFLLRHHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVMEQRSQILKQMHLTKNEREREIQSITDESRGSIRRKNPANTRLRLNVPEETAGDSEEKPEEEVQLIHDQSAPSCPSSSPSPGEEPEGEGETDPEKVHLTWTKDKSVAEKNKGPSPVSSEGIKDFFSMKPEWENLNQSNVRRMHTAVRLNEVIVKKSRDAKLVLLNMPGPPRNRNGDENYMEFLEVLTEHLDRVMLVRGGGREVITIYS

Target ORF information:

RefSeq Version	NM_001134771.2
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 12 member 5 (SLC12A5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134771.2

ORF Insert Sequence:

ATGAGCCGCA GGTTCACGGT CACCTCGCTG CCCCCCGCAG GGCCCGCCAG AAGCCCTGAC 
CCAGAGTCCC GCCGGCATTC GGTCGCAGAC CCCCGCCACC TCCCGGGGGA AGACGTCAAA 
GGTGATGGCA ACCCCAAGGA AAGCAGTCCC TTCATCAACA GCACCGACAC AGAGAAGGGA 
AAGGAGTATG ATGGCAAGAA CATGGCCTTG TTTGAGGAGG AGATGGACAC CAGCCCTATG 
GTGTCCTCCT TGCTCAGTGG CCTGGCCAAC TACACCAACC TGCCCCAGGG AAGTAGGGAG 
CATGAAGAGG CAGAAAACAA TGAGGGTGGA AAAAAGAAGC CGGTGCAGGC CCCACGCATG 
GGCACCTTCA TGGGCGTGTA CCTGCCGTGC CTGCAGAACA TCTTTGGCGT CATCCTCTTC 
CTGCGGCTCA CCTGGGTGGT GGGCATTGCA GGCATCATGG AGTCCTTCTG CATGGTGTTC 
ATCTGCTGCT CCTGTACGAT GCTCACGGCC ATCTCCATGA GTGCAATTGC AACGAATGGT 
GTTGTGCCTG CTGGTGGCTC CTACTACATG ATTTCCAGGT CTCTGGGCCC AGAGTTTGGG 
GGTGCCGTGG GCCTCTGCTT CTACCTGGGC ACTACCTTTG CAGGAGCCAT GTACATCCTG 
GGCACCATCG AAATCCTGCT GGCTTACCTC TTCCCAGCCA TGGCCATCTT CAAGGCAGAA 
GATGCCAGTG GGGAGGCAGC AGCCATGCTG AACAACATGC GTGTTTACGG CACCTGTGTG 
CTCACCTGCA TGGCCACTGT GGTGTTTGTG GGTGTCAAGT ATGTCAACAA GTTTGCCCTT 
GTCTTCCTGG GTTGTGTCAT CCTCTCCATC CTGGCCATCT ATGCTGGGGT CATCAAGTCT 
GCCTTCGACC CACCCAACTT CCCGATCTGC CTCCTGGGTA ACCGCACGCT GTCTCGCCAT 
GGCTTTGATG TCTGTGCCAA GCTGGCTTGG GAAGGAAATG AGACGGTGAC CACACGGCTA 
TGGGGCCTTT TCTGCTCCTC TCGCTTCCTC AACGCCACCT GTGATGAATA CTTCACCCGA 
AACAATGTCA CAGAGATCCA GGGCATCCCT GGTGCTGCCA GTGGCCTCAT CAAAGAGAAC 
CTCTGGAGCT CCTACCTGAC CAAGGGCGTG ATTGTGGAGA GGAGTGGGAT GACCTCGGTG 
GGCCTGGCCG ATGGCACTCC TATCGACATG GACCACCCTT ATGTCTTCAG TGATATGACC 
TCCTACTTCA CCCTGCTGGT TGGCATCTAC TTCCCCTCAG TCACAGGGAT CATGGCTGGT 
TCTAACCGCT CTGGGGACCT GAGGGATGCC CAGAAGTCAA TCCCCACTGG CACCATCCTG 
GCCATCGCCA CCACCTCTGC TGTCTACATC AGCTCCGTTG TTCTGTTTGG GGCCTGCATT 
GAGGGGGTCG TCCTGCGGGA CAAGTTTGGC GAAGCTGTGA ATGGCAACCT CGTGGTGGGC 
ACTCTGGCCT GGCCATCTCC ATGGGTAATT GTCATCGGAT CCTTCTTCTC CACCTGTGGG 
GCTGGGCTGC AGAGCCTCAC GGGGGCCCCA CGCCTGCTGC AGGCCATCTC GAGGGATGGC 
ATTGTGCCCT TCCTGCAGGT CTTTGGCCAT GGCAAGGCCA ATGGAGAGCC GACCTGGGCC 
CTGCTCCTGA CTGCCTGCAT CTGCGAGATT GGCATCCTCA TTGCATCCCT CGACGAGGTG 
GCCCCCATCC TCTCTATGTT CTTCCTGATG TGCTACATGT TTGTGAATCT GGCCTGTGCA 
GTGCAGACGC TGCTGAGGAC ACCCAACTGG AGGCCACGCT TTCGATATTA CCACTGGACC 
CTCTCCTTCC TGGGCATGAG CCTCTGCCTG GCCCTCATGT TCATCTGCTC CTGGTATTAT 
GCACTGGTAG CCATGCTCAT TGCTGGACTC ATCTACAAGT ACATTGAGTA CCGTGGGGCA 
GAGAAGGAGT GGGGCGATGG GATACGAGGT CTGTCTCTCA GTGCGGCTCG CTATGCCCTC 
TTACGCCTGG AGGAAGGGCC CCCACACACC AAGAACTGGA GGCCACAGCT GCTGGTGCTG 
GTGCGTGTGG ACCAAGACCA GAATGTGGTG CACCCCCAGC TGCTCTCACT GACCTCCCAG 
CTGAAGGCGG GGAAGGGCCT GACCATCGTG GGCTCTGTCC TTGAGGGCAC CTTTCTGGAA 
AATCATCCAC AGGCCCAGCG GGCAGAAGAG TCTATCAGGC GCCTGATGGA GGCAGAGAAG 
GTGAAGGGCT TCTGCCAGGT GGTGATCTCC TCCAACTTGC GTGATGGCGT GTCCCATCTG 
ATCCAGTCCG GGGGCCTCGG GGGGCTGCAG CACAACACTG TGCTTGTTGG CTGGCCCCGC 
AACTGGCGCC AGAAGGAAGA TCATCAGACG TGGAGGAACT TCATTGAGCT GGTCCGGGAA 
ACCACAGCTG GCCACTTAGC CCTGCTGGTC ACCAAGAACG TTTCCATGTT TCCTGGGAAC 
CCTGAGCGCT TCTCTGAGGG CAGCATCGAC GTTTGGTGGA TTGTGCACGA TGGAGGCATG 
CTCATGCTGC TGCCCTTCCT GCTGCGGCAC CACAAGGTCT GGCGGAAGTG CAAGATGCGT 
ATCTTCACTG TGGCCCAGAT GGATGACAAT AGCATCCAGA TGAAGAAGGA TCTGACCACA 
TTTCTGTATC ATTTACGCAT CACTGCGGAG GTCGAGGTGG TGGAGATGCA TGAGAGCGAC 
ATCTCAGCTT ACACCTATGA GAAGACGTTG GTGATGGAGC AGCGTTCCCA GATCCTCAAA 
CAGATGCATT TAACCAAGAA TGAGCGGGAG CGGGAGATCC AGAGTATCAC AGATGAGTCA 
CGAGGCTCAA TCCGGAGAAA GAATCCAGCC AACACGCGGC TCCGCCTGAA CGTCCCAGAA 
GAGACGGCTG GTGACAGTGA AGAGAAGCCA GAGGAGGAGG TGCAGCTGAT CCACGATCAG 
AGTGCTCCCA GCTGCCCCAG CAGCTCCCCG TCCCCAGGGG AGGAGCCTGA GGGGGAAGGG 
GAGACAGATC CGGAGAAGGT GCATCTCACC TGGACCAAGG ACAAGTCGGT GGCAGAGAAG 
AATAAGGGCC CCAGTCCTGT CTCCTCTGAG GGCATCAAGG ACTTCTTCAG CATGAAGCCG 
GAGTGGGAGA ACTTGAACCA GTCCAACGTG CGGCGCATGC ACACGGCCGT GCGGCTGAAC 
GAGGTCATCG TGAAGAAATC CCGGGACGCC AAGCTTGTTT TGCTCAACAT GCCTGGGCCT 
CCCCGCAACC GCAATGGTGA TGAAAACTAC ATGGAGTTTC TCGAGGTCCT CACAGAGCAC 
CTGGACCGGG TGATGCTGGT CCGCGGCGGC GGCCGCGAGG TCATCACCAT CTACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A5 ( NM_020708.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_065759.1 Homo sapiens solute carrier family 12 member 5 (SLC12A5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu108528
Clone ID Related Accession (Same CDS sequence)	NM_020708.5
Accession Version	NM_020708.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577980800000
Organism	Homo sapiens(human)
Product	solute carrier family 12 member 5 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162458.10. On Jun 2, 2019 this sequence version replaced NM_020708.4. Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (2, also known as KCC2b) has a distinct N-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC132670.1, AF208159.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTAAACA ACCTGACGGA CTGCGAGGAC GGCGATGGGG GAGCCAACCC GGGTGATGGC 
AACCCCAAGG AAAGCAGTCC CTTCATCAAC AGCACCGACA CAGAGAAGGG AAAGGAGTAT 
GATGGCAAGA ACATGGCCTT GTTTGAGGAG GAGATGGACA CCAGCCCTAT GGTGTCCTCC 
TTGCTCAGTG GCCTGGCCAA CTACACCAAC CTGCCCCAGG GAAGTAGGGA GCATGAAGAG 
GCAGAAAACA ATGAGGGTGG AAAAAAGAAG CCGGTGCAGG CCCCACGCAT GGGCACCTTC 
ATGGGCGTGT ACCTGCCGTG CCTGCAGAAC ATCTTTGGCG TCATCCTCTT CCTGCGGCTC 
ACCTGGGTGG TGGGCATTGC AGGCATCATG GAGTCCTTCT GCATGGTGTT CATCTGCTGC 
TCCTGTACGA TGCTCACGGC CATCTCCATG AGTGCAATTG CAACGAATGG TGTTGTGCCT 
GCTGGTGGCT CCTACTACAT GATTTCCAGG TCTCTGGGCC CAGAGTTTGG GGGTGCCGTG 
GGCCTCTGCT TCTACCTGGG CACTACCTTT GCAGGAGCCA TGTACATCCT GGGCACCATC 
GAAATCCTGC TGGCTTACCT CTTCCCAGCC ATGGCCATCT TCAAGGCAGA AGATGCCAGT 
GGGGAGGCAG CAGCCATGCT GAACAACATG CGTGTTTACG GCACCTGTGT GCTCACCTGC 
ATGGCCACTG TGGTGTTTGT GGGTGTCAAG TATGTCAACA AGTTTGCCCT TGTCTTCCTG 
GGTTGTGTCA TCCTCTCCAT CCTGGCCATC TATGCTGGGG TCATCAAGTC TGCCTTCGAC 
CCACCCAACT TCCCGATCTG CCTCCTGGGT AACCGCACGC TGTCTCGCCA TGGCTTTGAT 
GTCTGTGCCA AGCTGGCTTG GGAAGGAAAT GAGACGGTGA CCACACGGCT ATGGGGCCTT 
TTCTGCTCCT CTCGCTTCCT CAACGCCACC TGTGATGAAT ACTTCACCCG AAACAATGTC 
ACAGAGATCC AGGGCATCCC TGGTGCTGCC AGTGGCCTCA TCAAAGAGAA CCTCTGGAGC 
TCCTACCTGA CCAAGGGCGT GATTGTGGAG AGGAGTGGGA TGACCTCGGT GGGCCTGGCC 
GATGGCACTC CTATCGACAT GGACCACCCT TATGTCTTCA GTGATATGAC CTCCTACTTC 
ACCCTGCTGG TTGGCATCTA CTTCCCCTCA GTCACAGGGA TCATGGCTGG TTCTAACCGC 
TCTGGGGACC TGAGGGATGC CCAGAAGTCA ATCCCCACTG GCACCATCCT GGCCATCGCC 
ACCACCTCTG CTGTCTACAT CAGCTCCGTT GTTCTGTTTG GGGCCTGCAT TGAGGGGGTC 
GTCCTGCGGG ACAAGTTTGG CGAAGCTGTG AATGGCAACC TCGTGGTGGG CACTCTGGCC 
TGGCCATCTC CATGGGTAAT TGTCATCGGA TCCTTCTTCT CCACCTGTGG GGCTGGGCTG 
CAGAGCCTCA CGGGGGCCCC ACGCCTGCTG CAGGCCATCT CGAGGGATGG CATTGTGCCC 
TTCCTGCAGG TCTTTGGCCA TGGCAAGGCC AATGGAGAGC CGACCTGGGC CCTGCTCCTG 
ACTGCCTGCA TCTGCGAGAT TGGCATCCTC ATTGCATCCC TCGACGAGGT GGCCCCCATC 
CTCTCTATGT TCTTCCTGAT GTGCTACATG TTTGTGAATC TGGCCTGTGC AGTGCAGACG 
CTGCTGAGGA CACCCAACTG GAGGCCACGC TTTCGATATT ACCACTGGAC CCTCTCCTTC 
CTGGGCATGA GCCTCTGCCT GGCCCTCATG TTCATCTGCT CCTGGTATTA TGCACTGGTA 
GCCATGCTCA TTGCTGGACT CATCTACAAG TACATTGAGT ACCGTGGGGC AGAGAAGGAG 
TGGGGCGATG GGATACGAGG TCTGTCTCTC AGTGCGGCTC GCTATGCCCT CTTACGCCTG 
GAGGAAGGGC CCCCACACAC CAAGAACTGG AGGCCACAGC TGCTGGTGCT GGTGCGTGTG 
GACCAAGACC AGAATGTGGT GCACCCCCAG CTGCTCTCAC TGACCTCCCA GCTGAAGGCG 
GGGAAGGGCC TGACCATCGT GGGCTCTGTC CTTGAGGGCA CCTTTCTGGA AAATCATCCA 
CAGGCCCAGC GGGCAGAAGA GTCTATCAGG CGCCTGATGG AGGCAGAGAA GGTGAAGGGC 
TTCTGCCAGG TGGTGATCTC CTCCAACTTG CGTGATGGCG TGTCCCATCT GATCCAGTCC 
GGGGGCCTCG GGGGGCTGCA GCACAACACT GTGCTTGTTG GCTGGCCCCG CAACTGGCGC 
CAGAAGGAAG ATCATCAGAC GTGGAGGAAC TTCATTGAGC TGGTCCGGGA AACCACAGCT 
GGCCACTTAG CCCTGCTGGT CACCAAGAAC GTTTCCATGT TTCCTGGGAA CCCTGAGCGC 
TTCTCTGAGG GCAGCATCGA CGTTTGGTGG ATTGTGCACG ATGGAGGCAT GCTCATGCTG 
CTGCCCTTCC TGCTGCGGCA CCACAAGGTC TGGCGGAAGT GCAAGATGCG TATCTTCACT 
GTGGCCCAGA TGGATGACAA TAGCATCCAG ATGAAGAAGG ATCTGACCAC ATTTCTGTAT 
CATTTACGCA TCACTGCGGA GGTCGAGGTG GTGGAGATGC ATGAGAGCGA CATCTCAGCT 
TACACCTATG AGAAGACGTT GGTGATGGAG CAGCGTTCCC AGATCCTCAA ACAGATGCAT 
TTAACCAAGA ATGAGCGGGA GCGGGAGATC CAGAGTATCA CAGATGAGTC ACGAGGCTCA 
ATCCGGAGAA AGAATCCAGC CAACACGCGG CTCCGCCTGA ACGTCCCAGA AGAGACGGCT 
GGTGACAGTG AAGAGAAGCC AGAGGAGGAG GTGCAGCTGA TCCACGATCA GAGTGCTCCC 
AGCTGCCCCA GCAGCTCCCC GTCCCCAGGG GAGGAGCCTG AGGGGGAAGG GGAGACAGAT 
CCGGAGAAGG TGCATCTCAC CTGGACCAAG GACAAGTCGG TGGCAGAGAA GAATAAGGGC 
CCCAGTCCTG TCTCCTCTGA GGGCATCAAG GACTTCTTCA GCATGAAGCC GGAGTGGGAG 
AACTTGAACC AGTCCAACGT GCGGCGCATG CACACGGCCG TGCGGCTGAA CGAGGTCATC 
GTGAAGAAAT CCCGGGACGC CAAGCTTGTT TTGCTCAACA TGCCTGGGCC TCCCCGCAAC 
CGCAATGGTG ATGAAAACTA CATGGAGTTT CTCGAGGTCC TCACAGAGCA CCTGGACCGG 
GTGATGCTGG TCCGCGGCGG CGGCCGCGAG GTCATCACCA TCTACTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065759.1
CDS	131..3481
Translation	MLNNLTDCEDGDGGANPGDGNPKESSPFINSTDTEKGKEYDGKNMALFEEEMDTSPMVSSLLSGLANYTNLPQGSREHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAEDASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIKSAFDPPNFPICLLGNRTLSRHGFDVCAKLAWEGNETVTTRLWGLFCSSRFLNATCDEYFTRNNVTEIQGIPGAASGLIKENLWSSYLTKGVIVERSGMTSVGLADGTPIDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQDQNVVHPQLLSLTSQLKAGKGLTIVGSVLEGTFLENHPQAQRAEESIRRLMEAEKVKGFCQVVISSNLRDGVSHLIQSGGLGGLQHNTVLVGWPRNWRQKEDHQTWRNFIELVRETTAGHLALLVTKNVSMFPGNPERFSEGSIDVWWIVHDGGMLMLLPFLLRHHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVMEQRSQILKQMHLTKNEREREIQSITDESRGSIRRKNPANTRLRLNVPEETAGDSEEKPEEEVQLIHDQSAPSCPSSSPSPGEEPEGEGETDPEKVHLTWTKDKSVAEKNKGPSPVSSEGIKDFFSMKPEWENLNQSNVRRMHTAVRLNEVIVKKSRDAKLVLLNMPGPPRNRNGDENYMEFLEVLTEHLDRVMLVRGGGREVITIYS

Target ORF information:

RefSeq Version	NM_020708.5
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 12 member 5 (SLC12A5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020708.5

ORF Insert Sequence:

ATGCTAAACA ACCTGACGGA CTGCGAGGAC GGCGATGGGG GAGCCAACCC GGGTGATGGC 
AACCCCAAGG AAAGCAGTCC CTTCATCAAC AGCACCGACA CAGAGAAGGG AAAGGAGTAT 
GATGGCAAGA ACATGGCCTT GTTTGAGGAG GAGATGGACA CCAGCCCTAT GGTGTCCTCC 
TTGCTCAGTG GCCTGGCCAA CTACACCAAC CTGCCCCAGG GAAGTAGGGA GCATGAAGAG 
GCAGAAAACA ATGAGGGTGG AAAAAAGAAG CCGGTGCAGG CCCCACGCAT GGGCACCTTC 
ATGGGCGTGT ACCTGCCGTG CCTGCAGAAC ATCTTTGGCG TCATCCTCTT CCTGCGGCTC 
ACCTGGGTGG TGGGCATTGC AGGCATCATG GAGTCCTTCT GCATGGTGTT CATCTGCTGC 
TCCTGTACGA TGCTCACGGC CATCTCCATG AGTGCAATTG CAACGAATGG TGTTGTGCCT 
GCTGGTGGCT CCTACTACAT GATTTCCAGG TCTCTGGGCC CAGAGTTTGG GGGTGCCGTG 
GGCCTCTGCT TCTACCTGGG CACTACCTTT GCAGGAGCCA TGTACATCCT GGGCACCATC 
GAAATCCTGC TGGCTTACCT CTTCCCAGCC ATGGCCATCT TCAAGGCAGA AGATGCCAGT 
GGGGAGGCAG CAGCCATGCT GAACAACATG CGTGTTTACG GCACCTGTGT GCTCACCTGC 
ATGGCCACTG TGGTGTTTGT GGGTGTCAAG TATGTCAACA AGTTTGCCCT TGTCTTCCTG 
GGTTGTGTCA TCCTCTCCAT CCTGGCCATC TATGCTGGGG TCATCAAGTC TGCCTTCGAC 
CCACCCAACT TCCCGATCTG CCTCCTGGGT AACCGCACGC TGTCTCGCCA TGGCTTTGAT 
GTCTGTGCCA AGCTGGCTTG GGAAGGAAAT GAGACGGTGA CCACACGGCT ATGGGGCCTT 
TTCTGCTCCT CTCGCTTCCT CAACGCCACC TGTGATGAAT ACTTCACCCG AAACAATGTC 
ACAGAGATCC AGGGCATCCC TGGTGCTGCC AGTGGCCTCA TCAAAGAGAA CCTCTGGAGC 
TCCTACCTGA CCAAGGGCGT GATTGTGGAG AGGAGTGGGA TGACCTCGGT GGGCCTGGCC 
GATGGCACTC CTATCGACAT GGACCACCCT TATGTCTTCA GTGATATGAC CTCCTACTTC 
ACCCTGCTGG TTGGCATCTA CTTCCCCTCA GTCACAGGGA TCATGGCTGG TTCTAACCGC 
TCTGGGGACC TGAGGGATGC CCAGAAGTCA ATCCCCACTG GCACCATCCT GGCCATCGCC 
ACCACCTCTG CTGTCTACAT CAGCTCCGTT GTTCTGTTTG GGGCCTGCAT TGAGGGGGTC 
GTCCTGCGGG ACAAGTTTGG CGAAGCTGTG AATGGCAACC TCGTGGTGGG CACTCTGGCC 
TGGCCATCTC CATGGGTAAT TGTCATCGGA TCCTTCTTCT CCACCTGTGG GGCTGGGCTG 
CAGAGCCTCA CGGGGGCCCC ACGCCTGCTG CAGGCCATCT CGAGGGATGG CATTGTGCCC 
TTCCTGCAGG TCTTTGGCCA TGGCAAGGCC AATGGAGAGC CGACCTGGGC CCTGCTCCTG 
ACTGCCTGCA TCTGCGAGAT TGGCATCCTC ATTGCATCCC TCGACGAGGT GGCCCCCATC 
CTCTCTATGT TCTTCCTGAT GTGCTACATG TTTGTGAATC TGGCCTGTGC AGTGCAGACG 
CTGCTGAGGA CACCCAACTG GAGGCCACGC TTTCGATATT ACCACTGGAC CCTCTCCTTC 
CTGGGCATGA GCCTCTGCCT GGCCCTCATG TTCATCTGCT CCTGGTATTA TGCACTGGTA 
GCCATGCTCA TTGCTGGACT CATCTACAAG TACATTGAGT ACCGTGGGGC AGAGAAGGAG 
TGGGGCGATG GGATACGAGG TCTGTCTCTC AGTGCGGCTC GCTATGCCCT CTTACGCCTG 
GAGGAAGGGC CCCCACACAC CAAGAACTGG AGGCCACAGC TGCTGGTGCT GGTGCGTGTG 
GACCAAGACC AGAATGTGGT GCACCCCCAG CTGCTCTCAC TGACCTCCCA GCTGAAGGCG 
GGGAAGGGCC TGACCATCGT GGGCTCTGTC CTTGAGGGCA CCTTTCTGGA AAATCATCCA 
CAGGCCCAGC GGGCAGAAGA GTCTATCAGG CGCCTGATGG AGGCAGAGAA GGTGAAGGGC 
TTCTGCCAGG TGGTGATCTC CTCCAACTTG CGTGATGGCG TGTCCCATCT GATCCAGTCC 
GGGGGCCTCG GGGGGCTGCA GCACAACACT GTGCTTGTTG GCTGGCCCCG CAACTGGCGC 
CAGAAGGAAG ATCATCAGAC GTGGAGGAAC TTCATTGAGC TGGTCCGGGA AACCACAGCT 
GGCCACTTAG CCCTGCTGGT CACCAAGAAC GTTTCCATGT TTCCTGGGAA CCCTGAGCGC 
TTCTCTGAGG GCAGCATCGA CGTTTGGTGG ATTGTGCACG ATGGAGGCAT GCTCATGCTG 
CTGCCCTTCC TGCTGCGGCA CCACAAGGTC TGGCGGAAGT GCAAGATGCG TATCTTCACT 
GTGGCCCAGA TGGATGACAA TAGCATCCAG ATGAAGAAGG ATCTGACCAC ATTTCTGTAT 
CATTTACGCA TCACTGCGGA GGTCGAGGTG GTGGAGATGC ATGAGAGCGA CATCTCAGCT 
TACACCTATG AGAAGACGTT GGTGATGGAG CAGCGTTCCC AGATCCTCAA ACAGATGCAT 
TTAACCAAGA ATGAGCGGGA GCGGGAGATC CAGAGTATCA CAGATGAGTC ACGAGGCTCA 
ATCCGGAGAA AGAATCCAGC CAACACGCGG CTCCGCCTGA ACGTCCCAGA AGAGACGGCT 
GGTGACAGTG AAGAGAAGCC AGAGGAGGAG GTGCAGCTGA TCCACGATCA GAGTGCTCCC 
AGCTGCCCCA GCAGCTCCCC GTCCCCAGGG GAGGAGCCTG AGGGGGAAGG GGAGACAGAT 
CCGGAGAAGG TGCATCTCAC CTGGACCAAG GACAAGTCGG TGGCAGAGAA GAATAAGGGC 
CCCAGTCCTG TCTCCTCTGA GGGCATCAAG GACTTCTTCA GCATGAAGCC GGAGTGGGAG 
AACTTGAACC AGTCCAACGT GCGGCGCATG CACACGGCCG TGCGGCTGAA CGAGGTCATC 
GTGAAGAAAT CCCGGGACGC CAAGCTTGTT TTGCTCAACA TGCCTGGGCC TCCCCGCAAC 
CGCAATGGTG ATGAAAACTA CATGGAGTTT CTCGAGGTCC TCACAGAGCA CCTGGACCGG 
GTGATGCTGG TCCGCGGCGG CGGCCGCGAG GTCATCACCA TCTACTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A5 ( XM_017027981.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883470.1 Homo sapiens solute carrier family 12 member 5 (SLC12A5), transcript variant X1, mRNA.

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CloneID	OHu93091
Clone ID Related Accession (Same CDS sequence)	XM_017027981.1
Accession Version	XM_017027981.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521907200000
Organism	Homo sapiens(human)
Product	solute carrier family 12 member 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGCA GGTTCACGGT CACCTCGCTG CCCCCCGCAG GGCCCGCCAG AAGCCCTGAC 
CCAGAGTCCC GCCGGCATTC GGTCGCAGAC CCCCGCCACC TCCCGGGGGA AGACGTCAAA 
GGTGATGGCA ACCCCAAGGA AAGCAGTCCC TTCATCAACA GCACCGACAC AGAGAAGGGA 
AAGGAGTATG ATGGCAAGAA CATGGCCTTG TTTGAGGAGG AGATGGACAC CAGCCCTATG 
GTGTCCTCCT TGCTCAGTGG CCTGGCCAAC TACACCAACC TGCCCCAGGG AAGTAGGGAG 
CATGAAGAGG CAGAAAACAA TGAGGGTGGA AAAAAGAAGC CGGTGCAGGC CCCACGCATG 
GGCACCTTCA TGGGCGTGTA CCTGCCGTGC CTGCAGAACA TCTTTGGCGT CATCCTCTTC 
CTGCGGCTCA CCTGGGTGGT GGGCATTGCA GGCATCATGG AGTCCTTCTG CATGGTGTTC 
ATCTGCTGCT CCTGTACGAT GCTCACGGCC ATCTCCATGA GTGCAATTGC AACGAATGGT 
GTTGTGCCTG CTGGTGGCTC CTACTACATG ATTTCCAGGT CTCTGGGCCC AGAGTTTGGG 
GGTGCCGTGG GCCTCTGCTT CTACCTGGGC ACTACCTTTG CAGGAGCCAT GTACATCCTG 
GGCACCATCG AAATCCTGCT GGCTTACCTC TTCCCAGCCA TGGCCATCTT CAAGGCAGAA 
GATGCCAGTG GGGAGGCAGC AGCCATGCTG AACAACATGC GTGTTTACGG CACCTGTGTG 
CTCACCTGCA TGGCCACTGT GGTGTTTGTG GGTGTCAAGT ATGTCAACAA GTTTGCCCTT 
GTCTTCCTGG GTTGTGTCAT CCTCTCCATC CTGGCCATCT ATGCTGGGGT CATCAAGTCT 
GCCTTCGACC CACCCAACTT CCCGATCTGC CTCCTGGGTA ACCGCACGCT GTCTCGCCAT 
GGCTTTGATG TCTGTGCCAA GCTGGCTTGG GAAGGAAATG AGACGGTGAC CACACGGCTA 
TGGGGCCTTT TCTGCTCCTC TCGCTTCCTC AACGCCACCT GTGATGAATA CTTCACCCGA 
AACAATGTCA CAGAGATCCA GGGCATCCCT GGTGCTGCCA GTGGCCTCAT CAAAGAGAAC 
CTCTGGAGCT CCTACCTGAC CAAGGGCGTG ATTGTGGAGA GGAGTGGGAT GACCTCGGTG 
GGCCTGGCCG ATGGCACTCC TATCGACATG GACCACCCTT ATGTCTTCAG TGATATGACC 
TCCTACTTCA CCCTGCTGGT TGGCATCTAC TTCCCCTCAG TCACAGGGAT CATGGCTGGT 
TCTAACCGCT CTGGGGACCT GAGGGATGCC CAGAAGTCAA TCCCCACTGG CACCATCCTG 
GCCATCGCCA CCACCTCTGC TGTCTACATC AGCTCCGTTG TTCTGTTTGG GGCCTGCATT 
GAGGGGGTCG TCCTGCGGGA CAAGTTTGGC GAAGCTGTGA ATGGCAACCT CGTGGTGGGC 
ACTCTGGCCT GGCCATCTCC ATGGGTAATT GTCATCGGAT CCTTCTTCTC CACCTGTGGG 
GCTGGGCTGC AGAGCCTCAC GGGGGCCCCA CGCCTGCTGC AGGCCATCTC GAGGGATGGC 
ATTGTGCCCT TCCTGCAGGT CTTTGGCCAT GGCAAGGCCA ATGGAGAGCC GACCTGGGCC 
CTGCTCCTGA CTGCCTGCAT CTGCGAGATT GGCATCCTCA TTGCATCCCT CGACGAGGTG 
GCCCCCATCC TCTCTATGTT CTTCCTGATG TGCTACATGT TTGTGAATCT GGCCTGTGCA 
GTGCAGACGC TGCTGAGGAC ACCCAACTGG AGGCCACGCT TTCGATATTA CCACTGGACC 
CTCTCCTTCC TGGGCATGAG CCTCTGCCTG GCCCTCATGT TCATCTGCTC CTGGTATTAT 
GCACTGGTAG CCATGCTCAT TGCTGGACTC ATCTACAAGT ACATTGAGTA CCGTGGGGCA 
GAGAAGGAGT GGGGCGATGG GATACGAGGT CTGTCTCTCA GTGCGGCTCG CTATGCCCTC 
TTACGCCTGG AGGAAGGGCC CCCACACACC AAGAACTGGA GGCCACAGCT GCTGGTGCTG 
GTGCGTGTGG ACCAAGACCA GAATGTGGTG CACCCCCAGC TGCTCTCACT GACCTCCCAG 
CTGAAGGCGG GGAAGGGCCT GACCATCGTG GGCTCTGTCC TTGAGGGCAC CTTTCTGGAA 
AATCATCCAC AGGCCCAGCG GGCAGAAGAG TCTATCAGGC GCCTGATGGA GGCAGAGAAG 
GTGAAGGGCT TCTGCCAGGT GGTGATCTCC TCCAACTTGC GTGATGGCGT GTCCCATCTG 
ATCCAGTCCG GGGGCCTCGG GGGGCTGCAG CACAACACTG TGCTTGTTGG CTGGCCCCGC 
AACTGGCGCC AGAAGGAAGA TCATCAGACG TGGAGGAACT TCATTGAGCT GGTCCGGGAA 
ACCACAGCTG GCCACTTAGC CCTGCTGGTC ACCAAGAACG TTTCCATGTT TCCTGGGAAC 
CCTGAGCGCT TCTCTGAGGG CAGCATCGAC GTTTGGTGGA TTGTGCACGA TGGAGGCATG 
CTCATGCTGC TGCCCTTCCT GCTGCGGCAC CACAAGGTCT GGCGGAAGTG CAAGATGCGT 
ATCTTCACTG TGGCCCAGAT GGATGACAAT AGCATCCAGA TGAAGAAGGA TCTGACCACA 
TTTCTGTATC ATTTACGCAT CACTGCGGAG GTCGAGGTGG TGGAGATGCA TGAGAGCGAC 
ATCTCAGCTT ACACCTATGA GAAGACGTTG GTGATGGAGC AGCGTTCCCA GATCCTCAAA 
CAGATGCATT TAACCAAGAA TGAGCGGGAG CGGGAGATCC AGAGTATCAC AGATGAGTCA 
CGAGGCTCAA TCCGGAGAAA GAATCCAGCC AACACGCGGC TCCGCCTGAA CGTCCCAGAA 
GAGACGGCTG GTGACAGTGA AGAGAAGCCA GAGGAGGAGG TGCAGCTGAT CCACGATCAG 
AGTGCTCCCA GCTGCCCCAG CAGCTCCCCG TCCCCAGGGG AGGAGCCTGA GGGGGAAGGG 
GAGACAGATC CGGAGAAGGT GCATCTCACC TGGACCAAGG ACAAGTCGGT GGCAGAGAAG 
AATAAGGGCC CCAGTCCTGT CTCCTCTGAG GGCATCAAGG ACTTCTTCAG CATGAAGCCG 
AACCAGTCCA ACGTGCGGCG CATGCACACG GCCGTGCGGC TGAACGAGGT CATCGTGAAG 
AAATCCCGGG ACGCCAAGCT TGTTTTGCTC AACATGCCTG GGCCTCCCCG CAACCGCAAT 
GGTGATGAAA ACTACATGGA GTTTCTCGAG GTCCTCACAG AGCACCTGGA CCGGGTGATG 
CTGGTCCGCG GCGGCGGCCG CGAGGTCATC ACCATCTACT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883470.1
CDS	77..3481
Translation	MSRRFTVTSLPPAGPARSPDPESRRHSVADPRHLPGEDVKGDGNPKESSPFINSTDTEKGKEYDGKNMALFEEEMDTSPMVSSLLSGLANYTNLPQGSREHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAEDASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIKSAFDPPNFPICLLGNRTLSRHGFDVCAKLAWEGNETVTTRLWGLFCSSRFLNATCDEYFTRNNVTEIQGIPGAASGLIKENLWSSYLTKGVIVERSGMTSVGLADGTPIDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQDQNVVHPQLLSLTSQLKAGKGLTIVGSVLEGTFLENHPQAQRAEESIRRLMEAEKVKGFCQVVISSNLRDGVSHLIQSGGLGGLQHNTVLVGWPRNWRQKEDHQTWRNFIELVRETTAGHLALLVTKNVSMFPGNPERFSEGSIDVWWIVHDGGMLMLLPFLLRHHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVMEQRSQILKQMHLTKNEREREIQSITDESRGSIRRKNPANTRLRLNVPEETAGDSEEKPEEEVQLIHDQSAPSCPSSSPSPGEEPEGEGETDPEKVHLTWTKDKSVAEKNKGPSPVSSEGIKDFFSMKPNQSNVRRMHTAVRLNEVIVKKSRDAKLVLLNMPGPPRNRNGDENYMEFLEVLTEHLDRVMLVRGGGREVITIYS

Target ORF information:

RefSeq Version	XM_017027981.1
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 12 member 5 (SLC12A5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017027981.1

ORF Insert Sequence:

ATGAGCCGCA GGTTCACGGT CACCTCGCTG CCCCCCGCAG GGCCCGCCAG AAGCCCTGAC 
CCAGAGTCCC GCCGGCATTC GGTCGCAGAC CCCCGCCACC TCCCGGGGGA AGACGTCAAA 
GGTGATGGCA ACCCCAAGGA AAGCAGTCCC TTCATCAACA GCACCGACAC AGAGAAGGGA 
AAGGAGTATG ATGGCAAGAA CATGGCCTTG TTTGAGGAGG AGATGGACAC CAGCCCTATG 
GTGTCCTCCT TGCTCAGTGG CCTGGCCAAC TACACCAACC TGCCCCAGGG AAGTAGGGAG 
CATGAAGAGG CAGAAAACAA TGAGGGTGGA AAAAAGAAGC CGGTGCAGGC CCCACGCATG 
GGCACCTTCA TGGGCGTGTA CCTGCCGTGC CTGCAGAACA TCTTTGGCGT CATCCTCTTC 
CTGCGGCTCA CCTGGGTGGT GGGCATTGCA GGCATCATGG AGTCCTTCTG CATGGTGTTC 
ATCTGCTGCT CCTGTACGAT GCTCACGGCC ATCTCCATGA GTGCAATTGC AACGAATGGT 
GTTGTGCCTG CTGGTGGCTC CTACTACATG ATTTCCAGGT CTCTGGGCCC AGAGTTTGGG 
GGTGCCGTGG GCCTCTGCTT CTACCTGGGC ACTACCTTTG CAGGAGCCAT GTACATCCTG 
GGCACCATCG AAATCCTGCT GGCTTACCTC TTCCCAGCCA TGGCCATCTT CAAGGCAGAA 
GATGCCAGTG GGGAGGCAGC AGCCATGCTG AACAACATGC GTGTTTACGG CACCTGTGTG 
CTCACCTGCA TGGCCACTGT GGTGTTTGTG GGTGTCAAGT ATGTCAACAA GTTTGCCCTT 
GTCTTCCTGG GTTGTGTCAT CCTCTCCATC CTGGCCATCT ATGCTGGGGT CATCAAGTCT 
GCCTTCGACC CACCCAACTT CCCGATCTGC CTCCTGGGTA ACCGCACGCT GTCTCGCCAT 
GGCTTTGATG TCTGTGCCAA GCTGGCTTGG GAAGGAAATG AGACGGTGAC CACACGGCTA 
TGGGGCCTTT TCTGCTCCTC TCGCTTCCTC AACGCCACCT GTGATGAATA CTTCACCCGA 
AACAATGTCA CAGAGATCCA GGGCATCCCT GGTGCTGCCA GTGGCCTCAT CAAAGAGAAC 
CTCTGGAGCT CCTACCTGAC CAAGGGCGTG ATTGTGGAGA GGAGTGGGAT GACCTCGGTG 
GGCCTGGCCG ATGGCACTCC TATCGACATG GACCACCCTT ATGTCTTCAG TGATATGACC 
TCCTACTTCA CCCTGCTGGT TGGCATCTAC TTCCCCTCAG TCACAGGGAT CATGGCTGGT 
TCTAACCGCT CTGGGGACCT GAGGGATGCC CAGAAGTCAA TCCCCACTGG CACCATCCTG 
GCCATCGCCA CCACCTCTGC TGTCTACATC AGCTCCGTTG TTCTGTTTGG GGCCTGCATT 
GAGGGGGTCG TCCTGCGGGA CAAGTTTGGC GAAGCTGTGA ATGGCAACCT CGTGGTGGGC 
ACTCTGGCCT GGCCATCTCC ATGGGTAATT GTCATCGGAT CCTTCTTCTC CACCTGTGGG 
GCTGGGCTGC AGAGCCTCAC GGGGGCCCCA CGCCTGCTGC AGGCCATCTC GAGGGATGGC 
ATTGTGCCCT TCCTGCAGGT CTTTGGCCAT GGCAAGGCCA ATGGAGAGCC GACCTGGGCC 
CTGCTCCTGA CTGCCTGCAT CTGCGAGATT GGCATCCTCA TTGCATCCCT CGACGAGGTG 
GCCCCCATCC TCTCTATGTT CTTCCTGATG TGCTACATGT TTGTGAATCT GGCCTGTGCA 
GTGCAGACGC TGCTGAGGAC ACCCAACTGG AGGCCACGCT TTCGATATTA CCACTGGACC 
CTCTCCTTCC TGGGCATGAG CCTCTGCCTG GCCCTCATGT TCATCTGCTC CTGGTATTAT 
GCACTGGTAG CCATGCTCAT TGCTGGACTC ATCTACAAGT ACATTGAGTA CCGTGGGGCA 
GAGAAGGAGT GGGGCGATGG GATACGAGGT CTGTCTCTCA GTGCGGCTCG CTATGCCCTC 
TTACGCCTGG AGGAAGGGCC CCCACACACC AAGAACTGGA GGCCACAGCT GCTGGTGCTG 
GTGCGTGTGG ACCAAGACCA GAATGTGGTG CACCCCCAGC TGCTCTCACT GACCTCCCAG 
CTGAAGGCGG GGAAGGGCCT GACCATCGTG GGCTCTGTCC TTGAGGGCAC CTTTCTGGAA 
AATCATCCAC AGGCCCAGCG GGCAGAAGAG TCTATCAGGC GCCTGATGGA GGCAGAGAAG 
GTGAAGGGCT TCTGCCAGGT GGTGATCTCC TCCAACTTGC GTGATGGCGT GTCCCATCTG 
ATCCAGTCCG GGGGCCTCGG GGGGCTGCAG CACAACACTG TGCTTGTTGG CTGGCCCCGC 
AACTGGCGCC AGAAGGAAGA TCATCAGACG TGGAGGAACT TCATTGAGCT GGTCCGGGAA 
ACCACAGCTG GCCACTTAGC CCTGCTGGTC ACCAAGAACG TTTCCATGTT TCCTGGGAAC 
CCTGAGCGCT TCTCTGAGGG CAGCATCGAC GTTTGGTGGA TTGTGCACGA TGGAGGCATG 
CTCATGCTGC TGCCCTTCCT GCTGCGGCAC CACAAGGTCT GGCGGAAGTG CAAGATGCGT 
ATCTTCACTG TGGCCCAGAT GGATGACAAT AGCATCCAGA TGAAGAAGGA TCTGACCACA 
TTTCTGTATC ATTTACGCAT CACTGCGGAG GTCGAGGTGG TGGAGATGCA TGAGAGCGAC 
ATCTCAGCTT ACACCTATGA GAAGACGTTG GTGATGGAGC AGCGTTCCCA GATCCTCAAA 
CAGATGCATT TAACCAAGAA TGAGCGGGAG CGGGAGATCC AGAGTATCAC AGATGAGTCA 
CGAGGCTCAA TCCGGAGAAA GAATCCAGCC AACACGCGGC TCCGCCTGAA CGTCCCAGAA 
GAGACGGCTG GTGACAGTGA AGAGAAGCCA GAGGAGGAGG TGCAGCTGAT CCACGATCAG 
AGTGCTCCCA GCTGCCCCAG CAGCTCCCCG TCCCCAGGGG AGGAGCCTGA GGGGGAAGGG 
GAGACAGATC CGGAGAAGGT GCATCTCACC TGGACCAAGG ACAAGTCGGT GGCAGAGAAG 
AATAAGGGCC CCAGTCCTGT CTCCTCTGAG GGCATCAAGG ACTTCTTCAG CATGAAGCCG 
AACCAGTCCA ACGTGCGGCG CATGCACACG GCCGTGCGGC TGAACGAGGT CATCGTGAAG 
AAATCCCGGG ACGCCAAGCT TGTTTTGCTC AACATGCCTG GGCCTCCCCG CAACCGCAAT 
GGTGATGAAA ACTACATGGA GTTTCTCGAG GTCCTCACAG AGCACCTGGA CCGGGTGATG 
CTGGTCCGCG GCGGCGGCCG CGAGGTCATC ACCATCTACT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A5 ( NM_001134771.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001128243.1 Homo sapiens solute carrier family 12 member 5 (SLC12A5), transcript variant 1, mRNA.

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CloneID	OHu09712
Clone ID Related Accession (Same CDS sequence)	NM_001134771.1
Accession Version	NM_001134771.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550505600000
Organism	Homo sapiens(human)
Product	solute carrier family 12 member 5 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA102113.1, BC132668.1, AF208159.1 and BC036329.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) encodes the longer isoform (1, also known as KCC2a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCGCA GGTTCACGGT CACCTCGCTG CCCCCCGCAG GGCCCGCCAG AAGCCCTGAC 
CCAGAGTCCC GCCGGCATTC GGTCGCAGAC CCCCGCCACC TCCCGGGGGA AGACGTCAAA 
GGTGATGGCA ACCCCAAGGA AAGCAGTCCC TTCATCAACA GCACCGACAC AGAGAAGGGA 
AAGGAGTATG ATGGCAAGAA CATGGCCTTG TTTGAGGAGG AGATGGACAC CAGCCCTATG 
GTGTCCTCCT TGCTCAGTGG CCTGGCCAAC TACACCAACC TGCCCCAGGG AAGTAGGGAG 
CATGAAGAGG CAGAAAACAA TGAGGGTGGA AAAAAGAAGC CGGTGCAGGC CCCACGCATG 
GGCACCTTCA TGGGCGTGTA CCTGCCGTGC CTGCAGAACA TCTTTGGCGT CATCCTCTTC 
CTGCGGCTCA CCTGGGTGGT GGGCATTGCA GGCATCATGG AGTCCTTCTG CATGGTGTTC 
ATCTGCTGCT CCTGTACGAT GCTCACGGCC ATCTCCATGA GTGCAATTGC AACGAATGGT 
GTTGTGCCTG CTGGTGGCTC CTACTACATG ATTTCCAGGT CTCTGGGCCC AGAGTTTGGG 
GGTGCCGTGG GCCTCTGCTT CTACCTGGGC ACTACCTTTG CAGGAGCCAT GTACATCCTG 
GGCACCATCG AAATCCTGCT GGCTTACCTC TTCCCAGCCA TGGCCATCTT CAAGGCAGAA 
GATGCCAGTG GGGAGGCAGC AGCCATGCTG AACAACATGC GTGTTTACGG CACCTGTGTG 
CTCACCTGCA TGGCCACTGT GGTGTTTGTG GGTGTCAAGT ATGTCAACAA GTTTGCCCTT 
GTCTTCCTGG GTTGTGTCAT CCTCTCCATC CTGGCCATCT ATGCTGGGGT CATCAAGTCT 
GCCTTCGACC CACCCAACTT CCCGATCTGC CTCCTGGGTA ACCGCACGCT GTCTCGCCAT 
GGCTTTGATG TCTGTGCCAA GCTGGCTTGG GAAGGAAATG AGACGGTGAC CACACGGCTA 
TGGGGCCTTT TCTGCTCCTC TCGCTTCCTC AACGCCACCT GTGATGAATA CTTCACCCGA 
AACAATGTCA CAGAGATCCA GGGCATCCCT GGTGCTGCCA GTGGCCTCAT CAAAGAGAAC 
CTCTGGAGCT CCTACCTGAC CAAGGGCGTG ATTGTGGAGA GGAGTGGGAT GACCTCGGTG 
GGCCTGGCCG ATGGCACTCC TATCGACATG GACCACCCTT ATGTCTTCAG TGATATGACC 
TCCTACTTCA CCCTGCTGGT TGGCATCTAC TTCCCCTCAG TCACAGGGAT CATGGCTGGT 
TCTAACCGCT CTGGGGACCT GAGGGATGCC CAGAAGTCAA TCCCCACTGG CACCATCCTG 
GCCATCGCCA CCACCTCTGC TGTCTACATC AGCTCCGTTG TTCTGTTTGG GGCCTGCATT 
GAGGGGGTCG TCCTGCGGGA CAAGTTTGGC GAAGCTGTGA ATGGCAACCT CGTGGTGGGC 
ACTCTGGCCT GGCCATCTCC ATGGGTAATT GTCATCGGAT CCTTCTTCTC CACCTGTGGG 
GCTGGGCTGC AGAGCCTCAC GGGGGCCCCA CGCCTGCTGC AGGCCATCTC GAGGGATGGC 
ATTGTGCCCT TCCTGCAGGT CTTTGGCCAT GGCAAGGCCA ATGGAGAGCC GACCTGGGCC 
CTGCTCCTGA CTGCCTGCAT CTGCGAGATT GGCATCCTCA TTGCATCCCT CGACGAGGTG 
GCCCCCATCC TCTCTATGTT CTTCCTGATG TGCTACATGT TTGTGAATCT GGCCTGTGCA 
GTGCAGACGC TGCTGAGGAC ACCCAACTGG AGGCCACGCT TTCGATATTA CCACTGGACC 
CTCTCCTTCC TGGGCATGAG CCTCTGCCTG GCCCTCATGT TCATCTGCTC CTGGTATTAT 
GCACTGGTAG CCATGCTCAT TGCTGGACTC ATCTACAAGT ACATTGAGTA CCGTGGGGCA 
GAGAAGGAGT GGGGCGATGG GATACGAGGT CTGTCTCTCA GTGCGGCTCG CTATGCCCTC 
TTACGCCTGG AGGAAGGGCC CCCACACACC AAGAACTGGA GGCCACAGCT GCTGGTGCTG 
GTGCGTGTGG ACCAAGACCA GAATGTGGTG CACCCCCAGC TGCTCTCACT GACCTCCCAG 
CTGAAGGCAG GGAAGGGCCT GACCATCGTG GGCTCTGTCC TTGAGGGCAC CTTTCTGGAA 
AATCATCCAC AGGCCCAGCG GGCAGAAGAG TCTATCAGGC GCCTGATGGA GGCAGAGAAG 
GTGAAGGGCT TCTGCCAGGT GGTGATCTCC TCCAACTTGC GTGATGGCGT GTCCCATCTG 
ATCCAGTCCG GGGGCCTCGG GGGGCTGCAG CACAACACTG TGCTTGTTGG CTGGCCCCGC 
AACTGGCGCC AGAAGGAAGA TCATCAGACG TGGAGGAACT TCATTGAGCT GGTCCGGGAA 
ACCACAGCTG GCCACTTAGC CCTGCTGGTC ACCAAGAACG TTTCCATGTT TCCTGGGAAC 
CCTGAGCGCT TCTCTGAGGG CAGCATCGAC GTTTGGTGGA TTGTGCACGA TGGAGGCATG 
CTCATGCTGC TGCCCTTCCT GCTGCGGCAC CACAAGGTCT GGCGGAAGTG CAAGATGCGT 
ATCTTCACTG TGGCCCAGAT GGATGACAAT AGCATCCAGA TGAAGAAGGA TCTGACCACA 
TTTCTGTATC ATTTACGCAT CACTGCGGAG GTCGAGGTGG TGGAGATGCA TGAGAGCGAC 
ATCTCAGCTT ACACCTATGA GAAGACGTTG GTGATGGAGC AGCGTTCCCA GATCCTCAAA 
CAGATGCATT TAACCAAGAA TGAGCGGGAG CGGGAGATCC AGAGTATCAC AGATGAGTCA 
CGAGGCTCAA TCCGGAGAAA GAATCCAGCC AACACGCGGC TCCGCCTGAA CGTCCCAGAA 
GAGACGGCTG GTGACAGTGA AGAGAAGCCA GAGGAGGAGG TGCAGCTGAT CCACGATCAG 
AGTGCTCCCA GCTGCCCCAG CAGCTCCCCG TCCCCAGGGG AGGAGCCTGA GGGGGAAGGG 
GAGACAGATC CGGAGAAGGT GCATCTCACC TGGACCAAGG ACAAGTCGGT GGCAGAGAAG 
AATAAGGGCC CCAGTCCTGT CTCCTCTGAG GGCATCAAGG ACTTCTTCAG CATGAAGCCG 
GAGTGGGAGA ACTTGAACCA GTCCAACGTG CGGCGCATGC ACACGGCCGT GCGGCTGAAC 
GAGGTCATCG TGAAGAAATC CCGGGACGCC AAGCTTGTTT TGCTCAACAT GCCTGGGCCT 
CCCCGCAACC GCAATGGTGA TGAAAACTAC ATGGAGTTTC TCGAGGTCCT CACAGAGCAC 
CTGGACCGGG TGATGCTGGT CCGCGGCGGC GGCCGCGAGG TCATCACCAT CTACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128243.1
CDS	77..3496
Translation	MSRRFTVTSLPPAGPARSPDPESRRHSVADPRHLPGEDVKGDGNPKESSPFINSTDTEKGKEYDGKNMALFEEEMDTSPMVSSLLSGLANYTNLPQGSREHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAEDASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIKSAFDPPNFPICLLGNRTLSRHGFDVCAKLAWEGNETVTTRLWGLFCSSRFLNATCDEYFTRNNVTEIQGIPGAASGLIKENLWSSYLTKGVIVERSGMTSVGLADGTPIDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQDQNVVHPQLLSLTSQLKAGKGLTIVGSVLEGTFLENHPQAQRAEESIRRLMEAEKVKGFCQVVISSNLRDGVSHLIQSGGLGGLQHNTVLVGWPRNWRQKEDHQTWRNFIELVRETTAGHLALLVTKNVSMFPGNPERFSEGSIDVWWIVHDGGMLMLLPFLLRHHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVMEQRSQILKQMHLTKNEREREIQSITDESRGSIRRKNPANTRLRLNVPEETAGDSEEKPEEEVQLIHDQSAPSCPSSSPSPGEEPEGEGETDPEKVHLTWTKDKSVAEKNKGPSPVSSEGIKDFFSMKPEWENLNQSNVRRMHTAVRLNEVIVKKSRDAKLVLLNMPGPPRNRNGDENYMEFLEVLTEHLDRVMLVRGGGREVITIYS

Target ORF information:

RefSeq Version	NM_001134771.1
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 12 member 5 (SLC12A5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134771.1

ORF Insert Sequence:

ATGAGCCGCA GGTTCACGGT CACCTCGCTG CCCCCCGCAG GGCCCGCCAG AAGCCCTGAC 
CCAGAGTCCC GCCGGCATTC GGTCGCAGAC CCCCGCCACC TCCCGGGGGA AGACGTCAAA 
GGTGATGGCA ACCCCAAGGA AAGCAGTCCC TTCATCAACA GCACCGACAC AGAGAAGGGA 
AAGGAGTATG ATGGCAAGAA CATGGCCTTG TTTGAGGAGG AGATGGACAC CAGCCCTATG 
GTGTCCTCCT TGCTCAGTGG CCTGGCCAAC TACACCAACC TGCCCCAGGG AAGTAGGGAG 
CATGAAGAGG CAGAAAACAA TGAGGGTGGA AAAAAGAAGC CGGTGCAGGC CCCACGCATG 
GGCACCTTCA TGGGCGTGTA CCTGCCGTGC CTGCAGAACA TCTTTGGCGT CATCCTCTTC 
CTGCGGCTCA CCTGGGTGGT GGGCATTGCA GGCATCATGG AGTCCTTCTG CATGGTGTTC 
ATCTGCTGCT CCTGTACGAT GCTCACGGCC ATCTCCATGA GTGCAATTGC AACGAATGGT 
GTTGTGCCTG CTGGTGGCTC CTACTACATG ATTTCCAGGT CTCTGGGCCC AGAGTTTGGG 
GGTGCCGTGG GCCTCTGCTT CTACCTGGGC ACTACCTTTG CAGGAGCCAT GTACATCCTG 
GGCACCATCG AAATCCTGCT GGCTTACCTC TTCCCAGCCA TGGCCATCTT CAAGGCAGAA 
GATGCCAGTG GGGAGGCAGC AGCCATGCTG AACAACATGC GTGTTTACGG CACCTGTGTG 
CTCACCTGCA TGGCCACTGT GGTGTTTGTG GGTGTCAAGT ATGTCAACAA GTTTGCCCTT 
GTCTTCCTGG GTTGTGTCAT CCTCTCCATC CTGGCCATCT ATGCTGGGGT CATCAAGTCT 
GCCTTCGACC CACCCAACTT CCCGATCTGC CTCCTGGGTA ACCGCACGCT GTCTCGCCAT 
GGCTTTGATG TCTGTGCCAA GCTGGCTTGG GAAGGAAATG AGACGGTGAC CACACGGCTA 
TGGGGCCTTT TCTGCTCCTC TCGCTTCCTC AACGCCACCT GTGATGAATA CTTCACCCGA 
AACAATGTCA CAGAGATCCA GGGCATCCCT GGTGCTGCCA GTGGCCTCAT CAAAGAGAAC 
CTCTGGAGCT CCTACCTGAC CAAGGGCGTG ATTGTGGAGA GGAGTGGGAT GACCTCGGTG 
GGCCTGGCCG ATGGCACTCC TATCGACATG GACCACCCTT ATGTCTTCAG TGATATGACC 
TCCTACTTCA CCCTGCTGGT TGGCATCTAC TTCCCCTCAG TCACAGGGAT CATGGCTGGT 
TCTAACCGCT CTGGGGACCT GAGGGATGCC CAGAAGTCAA TCCCCACTGG CACCATCCTG 
GCCATCGCCA CCACCTCTGC TGTCTACATC AGCTCCGTTG TTCTGTTTGG GGCCTGCATT 
GAGGGGGTCG TCCTGCGGGA CAAGTTTGGC GAAGCTGTGA ATGGCAACCT CGTGGTGGGC 
ACTCTGGCCT GGCCATCTCC ATGGGTAATT GTCATCGGAT CCTTCTTCTC CACCTGTGGG 
GCTGGGCTGC AGAGCCTCAC GGGGGCCCCA CGCCTGCTGC AGGCCATCTC GAGGGATGGC 
ATTGTGCCCT TCCTGCAGGT CTTTGGCCAT GGCAAGGCCA ATGGAGAGCC GACCTGGGCC 
CTGCTCCTGA CTGCCTGCAT CTGCGAGATT GGCATCCTCA TTGCATCCCT CGACGAGGTG 
GCCCCCATCC TCTCTATGTT CTTCCTGATG TGCTACATGT TTGTGAATCT GGCCTGTGCA 
GTGCAGACGC TGCTGAGGAC ACCCAACTGG AGGCCACGCT TTCGATATTA CCACTGGACC 
CTCTCCTTCC TGGGCATGAG CCTCTGCCTG GCCCTCATGT TCATCTGCTC CTGGTATTAT 
GCACTGGTAG CCATGCTCAT TGCTGGACTC ATCTACAAGT ACATTGAGTA CCGTGGGGCA 
GAGAAGGAGT GGGGCGATGG GATACGAGGT CTGTCTCTCA GTGCGGCTCG CTATGCCCTC 
TTACGCCTGG AGGAAGGGCC CCCACACACC AAGAACTGGA GGCCACAGCT GCTGGTGCTG 
GTGCGTGTGG ACCAAGACCA GAATGTGGTG CACCCCCAGC TGCTCTCACT GACCTCCCAG 
CTGAAGGCAG GGAAGGGCCT GACCATCGTG GGCTCTGTCC TTGAGGGCAC CTTTCTGGAA 
AATCATCCAC AGGCCCAGCG GGCAGAAGAG TCTATCAGGC GCCTGATGGA GGCAGAGAAG 
GTGAAGGGCT TCTGCCAGGT GGTGATCTCC TCCAACTTGC GTGATGGCGT GTCCCATCTG 
ATCCAGTCCG GGGGCCTCGG GGGGCTGCAG CACAACACTG TGCTTGTTGG CTGGCCCCGC 
AACTGGCGCC AGAAGGAAGA TCATCAGACG TGGAGGAACT TCATTGAGCT GGTCCGGGAA 
ACCACAGCTG GCCACTTAGC CCTGCTGGTC ACCAAGAACG TTTCCATGTT TCCTGGGAAC 
CCTGAGCGCT TCTCTGAGGG CAGCATCGAC GTTTGGTGGA TTGTGCACGA TGGAGGCATG 
CTCATGCTGC TGCCCTTCCT GCTGCGGCAC CACAAGGTCT GGCGGAAGTG CAAGATGCGT 
ATCTTCACTG TGGCCCAGAT GGATGACAAT AGCATCCAGA TGAAGAAGGA TCTGACCACA 
TTTCTGTATC ATTTACGCAT CACTGCGGAG GTCGAGGTGG TGGAGATGCA TGAGAGCGAC 
ATCTCAGCTT ACACCTATGA GAAGACGTTG GTGATGGAGC AGCGTTCCCA GATCCTCAAA 
CAGATGCATT TAACCAAGAA TGAGCGGGAG CGGGAGATCC AGAGTATCAC AGATGAGTCA 
CGAGGCTCAA TCCGGAGAAA GAATCCAGCC AACACGCGGC TCCGCCTGAA CGTCCCAGAA 
GAGACGGCTG GTGACAGTGA AGAGAAGCCA GAGGAGGAGG TGCAGCTGAT CCACGATCAG 
AGTGCTCCCA GCTGCCCCAG CAGCTCCCCG TCCCCAGGGG AGGAGCCTGA GGGGGAAGGG 
GAGACAGATC CGGAGAAGGT GCATCTCACC TGGACCAAGG ACAAGTCGGT GGCAGAGAAG 
AATAAGGGCC CCAGTCCTGT CTCCTCTGAG GGCATCAAGG ACTTCTTCAG CATGAAGCCG 
GAGTGGGAGA ACTTGAACCA GTCCAACGTG CGGCGCATGC ACACGGCCGT GCGGCTGAAC 
GAGGTCATCG TGAAGAAATC CCGGGACGCC AAGCTTGTTT TGCTCAACAT GCCTGGGCCT 
CCCCGCAACC GCAATGGTGA TGAAAACTAC ATGGAGTTTC TCGAGGTCCT CACAGAGCAC 
CTGGACCGGG TGATGCTGGT CCGCGGCGGC GGCCGCGAGG TCATCACCAT CTACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A5 ( NM_020708.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_065759.1 Homo sapiens solute carrier family 12 member 5 (SLC12A5), transcript variant 2, mRNA.

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CloneID	OHu10133
Clone ID Related Accession (Same CDS sequence)	NM_020708.4
Accession Version	NM_020708.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550505600000
Organism	Homo sapiens(human)
Product	solute carrier family 12 member 5 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC320473.1, BC132668.1, AF208159.1 and BC036329.1. On Sep 5, 2008 this sequence version replaced NM_020708.3. Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (2, also known as KCC2b) has a distinct N-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC132668.1, AF208159.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTAAACA ACCTGACGGA CTGCGAGGAC GGCGATGGGG GAGCCAACCC GGGTGATGGC 
AACCCCAAGG AAAGCAGTCC CTTCATCAAC AGCACCGACA CAGAGAAGGG AAAGGAGTAT 
GATGGCAAGA ACATGGCCTT GTTTGAGGAG GAGATGGACA CCAGCCCTAT GGTGTCCTCC 
TTGCTCAGTG GCCTGGCCAA CTACACCAAC CTGCCCCAGG GAAGTAGGGA GCATGAAGAG 
GCAGAAAACA ATGAGGGTGG AAAAAAGAAG CCGGTGCAGG CCCCACGCAT GGGCACCTTC 
ATGGGCGTGT ACCTGCCGTG CCTGCAGAAC ATCTTTGGCG TCATCCTCTT CCTGCGGCTC 
ACCTGGGTGG TGGGCATTGC AGGCATCATG GAGTCCTTCT GCATGGTGTT CATCTGCTGC 
TCCTGTACGA TGCTCACGGC CATCTCCATG AGTGCAATTG CAACGAATGG TGTTGTGCCT 
GCTGGTGGCT CCTACTACAT GATTTCCAGG TCTCTGGGCC CAGAGTTTGG GGGTGCCGTG 
GGCCTCTGCT TCTACCTGGG CACTACCTTT GCAGGAGCCA TGTACATCCT GGGCACCATC 
GAAATCCTGC TGGCTTACCT CTTCCCAGCC ATGGCCATCT TCAAGGCAGA AGATGCCAGT 
GGGGAGGCAG CAGCCATGCT GAACAACATG CGTGTTTACG GCACCTGTGT GCTCACCTGC 
ATGGCCACTG TGGTGTTTGT GGGTGTCAAG TATGTCAACA AGTTTGCCCT TGTCTTCCTG 
GGTTGTGTCA TCCTCTCCAT CCTGGCCATC TATGCTGGGG TCATCAAGTC TGCCTTCGAC 
CCACCCAACT TCCCGATCTG CCTCCTGGGT AACCGCACGC TGTCTCGCCA TGGCTTTGAT 
GTCTGTGCCA AGCTGGCTTG GGAAGGAAAT GAGACGGTGA CCACACGGCT ATGGGGCCTT 
TTCTGCTCCT CTCGCTTCCT CAACGCCACC TGTGATGAAT ACTTCACCCG AAACAATGTC 
ACAGAGATCC AGGGCATCCC TGGTGCTGCC AGTGGCCTCA TCAAAGAGAA CCTCTGGAGC 
TCCTACCTGA CCAAGGGCGT GATTGTGGAG AGGAGTGGGA TGACCTCGGT GGGCCTGGCC 
GATGGCACTC CTATCGACAT GGACCACCCT TATGTCTTCA GTGATATGAC CTCCTACTTC 
ACCCTGCTGG TTGGCATCTA CTTCCCCTCA GTCACAGGGA TCATGGCTGG TTCTAACCGC 
TCTGGGGACC TGAGGGATGC CCAGAAGTCA ATCCCCACTG GCACCATCCT GGCCATCGCC 
ACCACCTCTG CTGTCTACAT CAGCTCCGTT GTTCTGTTTG GGGCCTGCAT TGAGGGGGTC 
GTCCTGCGGG ACAAGTTTGG CGAAGCTGTG AATGGCAACC TCGTGGTGGG CACTCTGGCC 
TGGCCATCTC CATGGGTAAT TGTCATCGGA TCCTTCTTCT CCACCTGTGG GGCTGGGCTG 
CAGAGCCTCA CGGGGGCCCC ACGCCTGCTG CAGGCCATCT CGAGGGATGG CATTGTGCCC 
TTCCTGCAGG TCTTTGGCCA TGGCAAGGCC AATGGAGAGC CGACCTGGGC CCTGCTCCTG 
ACTGCCTGCA TCTGCGAGAT TGGCATCCTC ATTGCATCCC TCGACGAGGT GGCCCCCATC 
CTCTCTATGT TCTTCCTGAT GTGCTACATG TTTGTGAATC TGGCCTGTGC AGTGCAGACG 
CTGCTGAGGA CACCCAACTG GAGGCCACGC TTTCGATATT ACCACTGGAC CCTCTCCTTC 
CTGGGCATGA GCCTCTGCCT GGCCCTCATG TTCATCTGCT CCTGGTATTA TGCACTGGTA 
GCCATGCTCA TTGCTGGACT CATCTACAAG TACATTGAGT ACCGTGGGGC AGAGAAGGAG 
TGGGGCGATG GGATACGAGG TCTGTCTCTC AGTGCGGCTC GCTATGCCCT CTTACGCCTG 
GAGGAAGGGC CCCCACACAC CAAGAACTGG AGGCCACAGC TGCTGGTGCT GGTGCGTGTG 
GACCAAGACC AGAATGTGGT GCACCCCCAG CTGCTCTCAC TGACCTCCCA GCTGAAGGCA 
GGGAAGGGCC TGACCATCGT GGGCTCTGTC CTTGAGGGCA CCTTTCTGGA AAATCATCCA 
CAGGCCCAGC GGGCAGAAGA GTCTATCAGG CGCCTGATGG AGGCAGAGAA GGTGAAGGGC 
TTCTGCCAGG TGGTGATCTC CTCCAACTTG CGTGATGGCG TGTCCCATCT GATCCAGTCC 
GGGGGCCTCG GGGGGCTGCA GCACAACACT GTGCTTGTTG GCTGGCCCCG CAACTGGCGC 
CAGAAGGAAG ATCATCAGAC GTGGAGGAAC TTCATTGAGC TGGTCCGGGA AACCACAGCT 
GGCCACTTAG CCCTGCTGGT CACCAAGAAC GTTTCCATGT TTCCTGGGAA CCCTGAGCGC 
TTCTCTGAGG GCAGCATCGA CGTTTGGTGG ATTGTGCACG ATGGAGGCAT GCTCATGCTG 
CTGCCCTTCC TGCTGCGGCA CCACAAGGTC TGGCGGAAGT GCAAGATGCG TATCTTCACT 
GTGGCCCAGA TGGATGACAA TAGCATCCAG ATGAAGAAGG ATCTGACCAC ATTTCTGTAT 
CATTTACGCA TCACTGCGGA GGTCGAGGTG GTGGAGATGC ATGAGAGCGA CATCTCAGCT 
TACACCTATG AGAAGACGTT GGTGATGGAG CAGCGTTCCC AGATCCTCAA ACAGATGCAT 
TTAACCAAGA ATGAGCGGGA GCGGGAGATC CAGAGTATCA CAGATGAGTC ACGAGGCTCA 
ATCCGGAGAA AGAATCCAGC CAACACGCGG CTCCGCCTGA ACGTCCCAGA AGAGACGGCT 
GGTGACAGTG AAGAGAAGCC AGAGGAGGAG GTGCAGCTGA TCCACGATCA GAGTGCTCCC 
AGCTGCCCCA GCAGCTCCCC GTCCCCAGGG GAGGAGCCTG AGGGGGAAGG GGAGACAGAT 
CCGGAGAAGG TGCATCTCAC CTGGACCAAG GACAAGTCGG TGGCAGAGAA GAATAAGGGC 
CCCAGTCCTG TCTCCTCTGA GGGCATCAAG GACTTCTTCA GCATGAAGCC GGAGTGGGAG 
AACTTGAACC AGTCCAACGT GCGGCGCATG CACACGGCCG TGCGGCTGAA CGAGGTCATC 
GTGAAGAAAT CCCGGGACGC CAAGCTTGTT TTGCTCAACA TGCCTGGGCC TCCCCGCAAC 
CGCAATGGTG ATGAAAACTA CATGGAGTTT CTCGAGGTCC TCACAGAGCA CCTGGACCGG 
GTGATGCTGG TCCGCGGCGG CGGCCGCGAG GTCATCACCA TCTACTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065759.1
CDS	164..3514
Translation	MLNNLTDCEDGDGGANPGDGNPKESSPFINSTDTEKGKEYDGKNMALFEEEMDTSPMVSSLLSGLANYTNLPQGSREHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAEDASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIKSAFDPPNFPICLLGNRTLSRHGFDVCAKLAWEGNETVTTRLWGLFCSSRFLNATCDEYFTRNNVTEIQGIPGAASGLIKENLWSSYLTKGVIVERSGMTSVGLADGTPIDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQDQNVVHPQLLSLTSQLKAGKGLTIVGSVLEGTFLENHPQAQRAEESIRRLMEAEKVKGFCQVVISSNLRDGVSHLIQSGGLGGLQHNTVLVGWPRNWRQKEDHQTWRNFIELVRETTAGHLALLVTKNVSMFPGNPERFSEGSIDVWWIVHDGGMLMLLPFLLRHHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVMEQRSQILKQMHLTKNEREREIQSITDESRGSIRRKNPANTRLRLNVPEETAGDSEEKPEEEVQLIHDQSAPSCPSSSPSPGEEPEGEGETDPEKVHLTWTKDKSVAEKNKGPSPVSSEGIKDFFSMKPEWENLNQSNVRRMHTAVRLNEVIVKKSRDAKLVLLNMPGPPRNRNGDENYMEFLEVLTEHLDRVMLVRGGGREVITIYS

Target ORF information:

RefSeq Version	NM_020708.4
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 12 member 5 (SLC12A5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020708.4

ORF Insert Sequence:

ATGCTAAACA ACCTGACGGA CTGCGAGGAC GGCGATGGGG GAGCCAACCC GGGTGATGGC 
AACCCCAAGG AAAGCAGTCC CTTCATCAAC AGCACCGACA CAGAGAAGGG AAAGGAGTAT 
GATGGCAAGA ACATGGCCTT GTTTGAGGAG GAGATGGACA CCAGCCCTAT GGTGTCCTCC 
TTGCTCAGTG GCCTGGCCAA CTACACCAAC CTGCCCCAGG GAAGTAGGGA GCATGAAGAG 
GCAGAAAACA ATGAGGGTGG AAAAAAGAAG CCGGTGCAGG CCCCACGCAT GGGCACCTTC 
ATGGGCGTGT ACCTGCCGTG CCTGCAGAAC ATCTTTGGCG TCATCCTCTT CCTGCGGCTC 
ACCTGGGTGG TGGGCATTGC AGGCATCATG GAGTCCTTCT GCATGGTGTT CATCTGCTGC 
TCCTGTACGA TGCTCACGGC CATCTCCATG AGTGCAATTG CAACGAATGG TGTTGTGCCT 
GCTGGTGGCT CCTACTACAT GATTTCCAGG TCTCTGGGCC CAGAGTTTGG GGGTGCCGTG 
GGCCTCTGCT TCTACCTGGG CACTACCTTT GCAGGAGCCA TGTACATCCT GGGCACCATC 
GAAATCCTGC TGGCTTACCT CTTCCCAGCC ATGGCCATCT TCAAGGCAGA AGATGCCAGT 
GGGGAGGCAG CAGCCATGCT GAACAACATG CGTGTTTACG GCACCTGTGT GCTCACCTGC 
ATGGCCACTG TGGTGTTTGT GGGTGTCAAG TATGTCAACA AGTTTGCCCT TGTCTTCCTG 
GGTTGTGTCA TCCTCTCCAT CCTGGCCATC TATGCTGGGG TCATCAAGTC TGCCTTCGAC 
CCACCCAACT TCCCGATCTG CCTCCTGGGT AACCGCACGC TGTCTCGCCA TGGCTTTGAT 
GTCTGTGCCA AGCTGGCTTG GGAAGGAAAT GAGACGGTGA CCACACGGCT ATGGGGCCTT 
TTCTGCTCCT CTCGCTTCCT CAACGCCACC TGTGATGAAT ACTTCACCCG AAACAATGTC 
ACAGAGATCC AGGGCATCCC TGGTGCTGCC AGTGGCCTCA TCAAAGAGAA CCTCTGGAGC 
TCCTACCTGA CCAAGGGCGT GATTGTGGAG AGGAGTGGGA TGACCTCGGT GGGCCTGGCC 
GATGGCACTC CTATCGACAT GGACCACCCT TATGTCTTCA GTGATATGAC CTCCTACTTC 
ACCCTGCTGG TTGGCATCTA CTTCCCCTCA GTCACAGGGA TCATGGCTGG TTCTAACCGC 
TCTGGGGACC TGAGGGATGC CCAGAAGTCA ATCCCCACTG GCACCATCCT GGCCATCGCC 
ACCACCTCTG CTGTCTACAT CAGCTCCGTT GTTCTGTTTG GGGCCTGCAT TGAGGGGGTC 
GTCCTGCGGG ACAAGTTTGG CGAAGCTGTG AATGGCAACC TCGTGGTGGG CACTCTGGCC 
TGGCCATCTC CATGGGTAAT TGTCATCGGA TCCTTCTTCT CCACCTGTGG GGCTGGGCTG 
CAGAGCCTCA CGGGGGCCCC ACGCCTGCTG CAGGCCATCT CGAGGGATGG CATTGTGCCC 
TTCCTGCAGG TCTTTGGCCA TGGCAAGGCC AATGGAGAGC CGACCTGGGC CCTGCTCCTG 
ACTGCCTGCA TCTGCGAGAT TGGCATCCTC ATTGCATCCC TCGACGAGGT GGCCCCCATC 
CTCTCTATGT TCTTCCTGAT GTGCTACATG TTTGTGAATC TGGCCTGTGC AGTGCAGACG 
CTGCTGAGGA CACCCAACTG GAGGCCACGC TTTCGATATT ACCACTGGAC CCTCTCCTTC 
CTGGGCATGA GCCTCTGCCT GGCCCTCATG TTCATCTGCT CCTGGTATTA TGCACTGGTA 
GCCATGCTCA TTGCTGGACT CATCTACAAG TACATTGAGT ACCGTGGGGC AGAGAAGGAG 
TGGGGCGATG GGATACGAGG TCTGTCTCTC AGTGCGGCTC GCTATGCCCT CTTACGCCTG 
GAGGAAGGGC CCCCACACAC CAAGAACTGG AGGCCACAGC TGCTGGTGCT GGTGCGTGTG 
GACCAAGACC AGAATGTGGT GCACCCCCAG CTGCTCTCAC TGACCTCCCA GCTGAAGGCA 
GGGAAGGGCC TGACCATCGT GGGCTCTGTC CTTGAGGGCA CCTTTCTGGA AAATCATCCA 
CAGGCCCAGC GGGCAGAAGA GTCTATCAGG CGCCTGATGG AGGCAGAGAA GGTGAAGGGC 
TTCTGCCAGG TGGTGATCTC CTCCAACTTG CGTGATGGCG TGTCCCATCT GATCCAGTCC 
GGGGGCCTCG GGGGGCTGCA GCACAACACT GTGCTTGTTG GCTGGCCCCG CAACTGGCGC 
CAGAAGGAAG ATCATCAGAC GTGGAGGAAC TTCATTGAGC TGGTCCGGGA AACCACAGCT 
GGCCACTTAG CCCTGCTGGT CACCAAGAAC GTTTCCATGT TTCCTGGGAA CCCTGAGCGC 
TTCTCTGAGG GCAGCATCGA CGTTTGGTGG ATTGTGCACG ATGGAGGCAT GCTCATGCTG 
CTGCCCTTCC TGCTGCGGCA CCACAAGGTC TGGCGGAAGT GCAAGATGCG TATCTTCACT 
GTGGCCCAGA TGGATGACAA TAGCATCCAG ATGAAGAAGG ATCTGACCAC ATTTCTGTAT 
CATTTACGCA TCACTGCGGA GGTCGAGGTG GTGGAGATGC ATGAGAGCGA CATCTCAGCT 
TACACCTATG AGAAGACGTT GGTGATGGAG CAGCGTTCCC AGATCCTCAA ACAGATGCAT 
TTAACCAAGA ATGAGCGGGA GCGGGAGATC CAGAGTATCA CAGATGAGTC ACGAGGCTCA 
ATCCGGAGAA AGAATCCAGC CAACACGCGG CTCCGCCTGA ACGTCCCAGA AGAGACGGCT 
GGTGACAGTG AAGAGAAGCC AGAGGAGGAG GTGCAGCTGA TCCACGATCA GAGTGCTCCC 
AGCTGCCCCA GCAGCTCCCC GTCCCCAGGG GAGGAGCCTG AGGGGGAAGG GGAGACAGAT 
CCGGAGAAGG TGCATCTCAC CTGGACCAAG GACAAGTCGG TGGCAGAGAA GAATAAGGGC 
CCCAGTCCTG TCTCCTCTGA GGGCATCAAG GACTTCTTCA GCATGAAGCC GGAGTGGGAG 
AACTTGAACC AGTCCAACGT GCGGCGCATG CACACGGCCG TGCGGCTGAA CGAGGTCATC 
GTGAAGAAAT CCCGGGACGC CAAGCTTGTT TTGCTCAACA TGCCTGGGCC TCCCCGCAAC 
CGCAATGGTG ATGAAAACTA CATGGAGTTT CTCGAGGTCC TCACAGAGCA CCTGGACCGG 
GTGATGCTGG TCCGCGGCGG CGGCCGCGAG GTCATCACCA TCTACTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Methylation of cation-chloride cotransporters NKCC1 and KCC2 in patients with juvenile myoclonic epilepsy.
Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology40(5)1007-1013(2019 May)
Genç F,Kara M,Ünal Y,Uygur Küçükseymen E,Biçer Gömceli Y,Kaynar T,Tosun K,Kutlu G

Brain microstructural abnormalities correlate with KCC2 downregulation in refractory epilepsy.
Neuroreport30(6)409-414(2019 04)
Gharaylou Z,Oghabian MA,Azizi Z,Hadjighassem M

Phosphoregulation of the intracellular termini of K
The Journal of biological chemistry293(44)16984-16993(2018 11)
Cordshagen A,Busch W,Winklhofer M,Nothwang HG,Hartmann AM

The neuronal K
Scientific reports7(1)1732(2017 05)
Kursan S,McMillen TS,Beesetty P,Dias-Junior E,Almutairi MM,Sajib AA,Kozak JA,Aguilar-Bryan L,Di Fulvio M

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SLC12A5 cDNA ORF clone, Homo sapiens(human)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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