SLC12A5B cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SLC12A5B
Entrez Gene ID	797331
Full Name	solute carrier family 12 (potassium/chloride transporter), member 5b
Synonyms	si:dkey-253e7.2
General protein information	Preferred Names solute carrier family 12 (potassium/chloride transporter), member 5b Names uncharacterized protein LOC797331 potassium/chloride cotransporter kcc2 slc12a5 zfkcc2
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009304148.3	XP_009302423.1	uncharacterized protein LOC797331 isoform X1
NM_001302243.1	NP_001289172.1	uncharacterized protein LOC797331
XM_009304148.2	XP_009302423.1	uncharacterized protein LOC797331 isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	SLC12A5	XP_001236722.3
Macaca mulatta (Rhesus monkey)	SLC12A5	XP_001104798.1
Bos taurus (cattle)	SLC12A5	NP_001193309.1
Pan troglodytes (chimpanzee)	SLC12A5	XP_003317037.1
Rattus norvegicus (Norway rat)	Slc12a5	NP_599190.1
Oryza sativa (rice)	Os08g0323700	NP_001061543.1
Danio rerio (zebrafish)	slc12a5b	XP_003199160.2
Danio rerio (zebrafish)	LOC572215	XP_701000.5
Homo sapiens (human)	SLC12A5	NP_065759.1
Canis lupus familiaris (dog)	SLC12A5	XP_005635409.1
Mus musculus (house mouse)	Slc12a5	NP_065066.2
Arabidopsis thaliana (thale cress)	CCC1	NP_174333.2
Xenopus tropicalis (tropical clawed frog)	slc12a5	NP_001072306.2

Gene Ontology
Bibliography

Related articles in PubMed

γ-Aminobutyric acid receptor alpha 1 subunit loss of function causes genetic generalized epilepsy by impairing inhibitory network neurodevelopment.
Samarut É, Swaminathan A, Riché R, Liao M, Hassan-Abdi R, Renault S, Allard M, Dufour L, Cossette P, Soussi-Yanicostas N, Drapeau P
Epilepsia59(11)2061-2074(2018 11)

Non-canonical mTOR-Independent Role of DEPDC5 in Regulating GABAergic Network Development.
Swaminathan A, Hassan-Abdi R, Renault S, Siekierska A, Riché R, Liao M, de Witte PAM, Yanicostas C, Soussi-Yanicostas N, Drapeau P, Samarut É
Current biology : CB28(12)1924-1937.e5(2018 06)

Neurotransmitter-mediated activity spatially controls neuronal migration in the zebrafish cerebellum.
Theisen U, Hennig C, Ring T, Schnabel R, Köster RW
PLoS biology16(1)e2002226(2018 01)

Glycine is able to induce both a motility speed in- and decrease during zebrafish neuronal migration.
Theisen U, Hey S, Hennig CD, Schnabel R, Köster RW
Communicative & integrative biology11(3)1-7(2018)

Evolution of complexity in the zebrafish synapse proteome.
Bayés À, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 03)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SLC12A5B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC12A5B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa47179	NM_001302243.1 Latest version!	Danio rerio solute carrier family 12 member 5b (slc12a5b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa47178	XM_009304148.3 Latest version!	Danio rerio solute carrier family 12 (potassium/chloride transporter), member 5b (slc12a5b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa47178	XM_009304148.2	Danio rerio solute carrier family 12 (potassium/chloride transporter), member 5b (slc12a5b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SLC12A5B ( NM_001302243.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001289172.1 Danio rerio solute carrier family 12 member 5b (slc12a5b), mRNA.

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CloneID	ODa47179
Clone ID Related Accession (Same CDS sequence)	NM_001302243.1
Accession Version	NM_001302243.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1554566400000
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC797331
Comment	Comment: PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from JN688966.1. On Oct 9, 2014 this sequence version replaced XM_003199112.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: JN688966.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168447, SAMEA2168448 [ECO:0000350] ##Evidence-Data-END##

ATGATGAACA ACCTCAATGA ATGTGAAGAA GGCGATGGGG GACCCAACAG CCAGGGAGAT 
GCAACATCCA AGGAAAGCAG CCCTTTCATC AACAGCAGCA CCAGTGACGT AGAAAAAAGC 
CAGCAGTATG ATGGGAAAAA CATGGCTCTG TTTGAGGAGG AGATGGACAC TAGTCCCATG 
GTATCTTCTC TGCTGAGCAG TCTTGCCAAC TACTCCAACC TGACACAGGG CAGTAAAGAG 
CATGAAGAGG CAGAAAACAA TGAGGAGGCA CGCAAGAAAG CTGCACAGGC TCCTCGTATG 
GGCACAATAA TGGGCGTTTA CCTACCATGT CTTCAAAACA TCCTTGGGGT GATTTTGTTC 
CTCAGGATGA CCTGGCTAGT GGGTGTTGGA GGCGTCCTGG GAACCTTCAC CGTCGTCTTC 
ATGTGCTGCT CCACGACTAT GCTGACTGCT ACCTCAATGA GTGCTATTGC CACCAATGGA 
GTTGTGCCAG CTGGAGGTTC GTACTACATG ATCTCTCGAT CACTCGGACC GGAGTTTGGA 
GGAGCTGTTG GCATTTGTTT CTTCTTGGGC ACCACTTTTG CAGGAGCCAT GTACATCTTG 
GGGTGCATCG AAATCCTGCT GATCTACATT GTTCCATCAG CTGCCATATT CAAAATGGAG 
GGTCTTGAGG GGTCAGAAGC CGAGGCAGCG CTGTTGAATA ACATGCGCGT GTACGGTACC 
ATCGTTCTCA CCTTCATGGC TATAGTCGTC TTTGTTGGTG TGAAGTATGT CAACAAGTTG 
GCTCTTGTGT TCCTCGCCTG TGTCATTCTC TCCATCCTGG CTATCTACGC GGGGGTCATC 
AAGACTTCTT TTGATCCACC TGACTTTCCG GTGTGTGTGC TTGGGAACCG AACTCTTGTG 
TCAAAGGCGT ATGACATTTG TGCCAAGACC ATAGAGCGGG GGAATGCCAC CATCACTACC 
AAGCTTTGGA GATCCTTCTG TGACTCTGAG TTCCTGAACG CCACCTGTGA TGAGTACTTT 
GTTAACAACA ATATCAGTCA GATCCAGGGA ATCCCTGGAG TCACCAGTGG CATCCTGGCA 
GAAAACCTTT TCAGTGGCTA TATGGAGAAA AATTCAGTCT TAGAGAAGAG AGGTCTACAG 
GCAGTACAGG ACCCTGAACT CCCAGTAACC AACAGTAACC GATATGTGCT GGCTGATATC 
ACCAGTTTCT TCACCCTGCT GGTTGGAATC TACTTCCCCT CTGTCACAGG TATCATGGCA 
GGTTCCAACC GTTCTGGTGA CCTGCAGGAT GCTCAGAAGT CCATCCCTGT TGGTACCATC 
TTGGCTATCA CAACCACCTC TATCATCTAC ATGTCTAGTG TAATTCTGTT TGGAGCCTGT 
GTAGATGGAG TTGTTTTGAG GGACAAATTT GGAGAAGGTG TCAGTGGTAA CCTGGTTATT 
GGCACGTTGG CATGGCCTTC ACCCTGGGTC ATTGTGTTTG GCTCCTTCTT CTCCACCTGT 
GGGGCCGGGC TTCAAAGTTT GACAGGAGCA CCCCGCCTCC TTCAAGCTAT TGCCCGAGAT 
GGCATCATTC CTTTCCTCAG GGTGTTTGGT CATGGCAAAG CTAACGGTGA ACCTACATGG 
GCTCTCCTTC TGACAGCCTG CATCTGTGAA AGTGGCATCC TCATTGCCTC CCTGGATGCA 
GTCGCACCTA TTCTGTCCAT GTTCTTTCTC ATGTGTTACA TGTTCGTAAA CCTGGCCTGT 
GCACTGCAAA CTCTCCTCAG AACCCCAAAC TGGAGACCAC GCTTTAAGTT TTACCATTGG 
GCTTTGTCAC TGTTGGGCAT GAGCTTGTGT CTTACCCTGA TGTTCCTGTG CTCCTGGTAC 
TATGCTATTG TTGCCATGGT AATCGCCGGC TGCATCTACA AATACATTGA ATTCTGCGGA 
GCAGAGAAAG AATGGGGTGA TGGAATTCGT GGTATCTCTT TGAGCGCTGC TCGTTTTGCT 
CTGATGAGGT TGGAGGAAGG TCCTCCTCAC ACTAAAAACT GGAGGCCCCA GATTTTGGTG 
CTCACAACTT TGGATGGGGA GCAGAACGTG GAGCAGCCTC GACTGCTGTC CCTGACCAGT 
CAGCTGAAAG CAGGCAAAGG TTTGACCATT GTAGGAGCGT GTATAGAAGG CACCTACTTA 
AACAACCAGC CAAAGACTCA GAAAGCAGAT CAGTCCCTAA GGAAGCTGAT GGAGGTTGAG 
AAGGTGAAGG GATTCAGTCA AGTGGTGATC TCTTCTAACC TGCGGGATGC CACCTCACAC 
CTAATTCAAG CTGGTGGTCT GGGTGGGCTA CGCCACAATA CTGTTCTGGT CAGCTTTCCC 
AAGAACTGGA AACAGGCGGA GGAGCACCAC CGTTGCAGGA ACTTTATTGA GGTTGTTAGG 
GAGACCACAG CAGGTCACAT GGCCTTACTG GTCCCGAAGA ACATTTCTGC ATATCCATCA 
AATGGAGAAC GTTTTACCGA GGGCCACATT GACGTGTGGT GGATTGTCCA CGATGGAGGC 
ATGCTCATGC TCCTTCCCTT TCTTCTCCGC CAGCATAAGG TTTGGAGGAA GTGCAAGATG 
CGTATCTTCA CTGTGGCTCA GATGGATGAC AACAGCATTC AGATGAAGAA AGACCTGATG 
ACGTTCCTTT ATCACCTGCG TCTTGATGCT GCAGTTGAAG TGGTTGAAAT GCTTGACAAT 
GACATCTCAG CTTACACCTA CGAAAAGACC CTGATGATGG AGCAGCGATC GCAAATCCTC 
AAGGAGATGC ATCTAACCAA GAATGAGCGG GAGAGAGAGA TTCAGAGCAT CACAGATGTG 
TCCCGTGGCT CTATTAGGCG TAAGAACCCA TCAAACCTGC ACCCCCAGAG GAGTATCGCT 
GAGGAGTCTG TGGCAGGAAG TGGTGAAGAA AAGCCTGAAG AGGAGGTTCA GCTCATTCAG 
GGAAAAAATG CCACCCCCAC GCCCACTAGC CCCACCTCCC CTACTGCTCC CACACCAGCA 
ACTATGTGCC CTGGAGAGGA GATTCAGATG ACCTGGACGG ACGATACGGA GAAGGCCAAT 
AACCCAGCAG TGGCCAATCC TGAAAACATA AAGGACATGT TCAACATGAA ACCGGAATGG 
GAGAACCTGA ATCAGTCCAA TGTGAGACGC ATGCACCATG CGCAGAAGCT CAATGAGGTC 
ATTGTGAAGA AATCGCAAGA AGCTAAACTA GTCCTGCTAA ACATGCCTGG ACCACCTAGG 
AACCGCACTG GAGAGGAGAA CTACATGGAG TTCCTCGAGG TCTTGACTGA AGGTCTCAAC 
AGGGTCCTTC TCGTGCGTGG AGGTGGACGT GAAGTCATTA CCATCTACTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289172.1
CDS	172..3525
Translation	MMNNLNECEEGDGGPNSQGDATSKESSPFINSSTSDVEKSQQYDGKNMALFEEEMDTSPMVSSLLSSLANYSNLTQGSKEHEEAENNEEARKKAAQAPRMGTIMGVYLPCLQNILGVILFLRMTWLVGVGGVLGTFTVVFMCCSTTMLTATSMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGICFFLGTTFAGAMYILGCIEILLIYIVPSAAIFKMEGLEGSEAEAALLNNMRVYGTIVLTFMAIVVFVGVKYVNKLALVFLACVILSILAIYAGVIKTSFDPPDFPVCVLGNRTLVSKAYDICAKTIERGNATITTKLWRSFCDSEFLNATCDEYFVNNNISQIQGIPGVTSGILAENLFSGYMEKNSVLEKRGLQAVQDPELPVTNSNRYVLADITSFFTLLVGIYFPSVTGIMAGSNRSGDLQDAQKSIPVGTILAITTTSIIYMSSVILFGACVDGVVLRDKFGEGVSGNLVIGTLAWPSPWVIVFGSFFSTCGAGLQSLTGAPRLLQAIARDGIIPFLRVFGHGKANGEPTWALLLTACICESGILIASLDAVAPILSMFFLMCYMFVNLACALQTLLRTPNWRPRFKFYHWALSLLGMSLCLTLMFLCSWYYAIVAMVIAGCIYKYIEFCGAEKEWGDGIRGISLSAARFALMRLEEGPPHTKNWRPQILVLTTLDGEQNVEQPRLLSLTSQLKAGKGLTIVGACIEGTYLNNQPKTQKADQSLRKLMEVEKVKGFSQVVISSNLRDATSHLIQAGGLGGLRHNTVLVSFPKNWKQAEEHHRCRNFIEVVRETTAGHMALLVPKNISAYPSNGERFTEGHIDVWWIVHDGGMLMLLPFLLRQHKVWRKCKMRIFTVAQMDDNSIQMKKDLMTFLYHLRLDAAVEVVEMLDNDISAYTYEKTLMMEQRSQILKEMHLTKNEREREIQSITDVSRGSIRRKNPSNLHPQRSIAEESVAGSGEEKPEEEVQLIQGKNATPTPTSPTSPTAPTPATMCPGEEIQMTWTDDTEKANNPAVANPENIKDMFNMKPEWENLNQSNVRRMHHAQKLNEVIVKKSQEAKLVLLNMPGPPRNRTGEENYMEFLEVLTEGLNRVLLVRGGGREVITIYS

Target ORF information:

RefSeq Version	NM_001302243.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio solute carrier family 12 member 5b (slc12a5b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302243.1

ORF Insert Sequence:

ATGATGAACA ACCTCAATGA ATGTGAAGAA GGCGATGGGG GACCCAACAG CCAGGGAGAT 
GCAACATCCA AGGAAAGCAG CCCTTTCATC AACAGCAGCA CCAGTGACGT AGAAAAAAGC 
CAGCAGTATG ATGGGAAAAA CATGGCTCTG TTTGAGGAGG AGATGGACAC TAGTCCCATG 
GTATCTTCTC TGCTGAGCAG TCTTGCCAAC TACTCCAACC TGACACAGGG CAGTAAAGAG 
CATGAAGAGG CAGAAAACAA TGAGGAGGCA CGCAAGAAAG CTGCACAGGC TCCTCGTATG 
GGCACAATAA TGGGCGTTTA CCTACCATGT CTTCAAAACA TCCTTGGGGT GATTTTGTTC 
CTCAGGATGA CCTGGCTAGT GGGTGTTGGA GGCGTCCTGG GAACCTTCAC CGTCGTCTTC 
ATGTGCTGCT CCACGACTAT GCTGACTGCT ACCTCAATGA GTGCTATTGC CACCAATGGA 
GTTGTGCCAG CTGGAGGTTC GTACTACATG ATCTCTCGAT CACTCGGACC GGAGTTTGGA 
GGAGCTGTTG GCATTTGTTT CTTCTTGGGC ACCACTTTTG CAGGAGCCAT GTACATCTTG 
GGGTGCATCG AAATCCTGCT GATCTACATT GTTCCATCAG CTGCCATATT CAAAATGGAG 
GGTCTTGAGG GGTCAGAAGC CGAGGCAGCG CTGTTGAATA ACATGCGCGT GTACGGTACC 
ATCGTTCTCA CCTTCATGGC TATAGTCGTC TTTGTTGGTG TGAAGTATGT CAACAAGTTG 
GCTCTTGTGT TCCTCGCCTG TGTCATTCTC TCCATCCTGG CTATCTACGC GGGGGTCATC 
AAGACTTCTT TTGATCCACC TGACTTTCCG GTGTGTGTGC TTGGGAACCG AACTCTTGTG 
TCAAAGGCGT ATGACATTTG TGCCAAGACC ATAGAGCGGG GGAATGCCAC CATCACTACC 
AAGCTTTGGA GATCCTTCTG TGACTCTGAG TTCCTGAACG CCACCTGTGA TGAGTACTTT 
GTTAACAACA ATATCAGTCA GATCCAGGGA ATCCCTGGAG TCACCAGTGG CATCCTGGCA 
GAAAACCTTT TCAGTGGCTA TATGGAGAAA AATTCAGTCT TAGAGAAGAG AGGTCTACAG 
GCAGTACAGG ACCCTGAACT CCCAGTAACC AACAGTAACC GATATGTGCT GGCTGATATC 
ACCAGTTTCT TCACCCTGCT GGTTGGAATC TACTTCCCCT CTGTCACAGG TATCATGGCA 
GGTTCCAACC GTTCTGGTGA CCTGCAGGAT GCTCAGAAGT CCATCCCTGT TGGTACCATC 
TTGGCTATCA CAACCACCTC TATCATCTAC ATGTCTAGTG TAATTCTGTT TGGAGCCTGT 
GTAGATGGAG TTGTTTTGAG GGACAAATTT GGAGAAGGTG TCAGTGGTAA CCTGGTTATT 
GGCACGTTGG CATGGCCTTC ACCCTGGGTC ATTGTGTTTG GCTCCTTCTT CTCCACCTGT 
GGGGCCGGGC TTCAAAGTTT GACAGGAGCA CCCCGCCTCC TTCAAGCTAT TGCCCGAGAT 
GGCATCATTC CTTTCCTCAG GGTGTTTGGT CATGGCAAAG CTAACGGTGA ACCTACATGG 
GCTCTCCTTC TGACAGCCTG CATCTGTGAA AGTGGCATCC TCATTGCCTC CCTGGATGCA 
GTCGCACCTA TTCTGTCCAT GTTCTTTCTC ATGTGTTACA TGTTCGTAAA CCTGGCCTGT 
GCACTGCAAA CTCTCCTCAG AACCCCAAAC TGGAGACCAC GCTTTAAGTT TTACCATTGG 
GCTTTGTCAC TGTTGGGCAT GAGCTTGTGT CTTACCCTGA TGTTCCTGTG CTCCTGGTAC 
TATGCTATTG TTGCCATGGT AATCGCCGGC TGCATCTACA AATACATTGA ATTCTGCGGA 
GCAGAGAAAG AATGGGGTGA TGGAATTCGT GGTATCTCTT TGAGCGCTGC TCGTTTTGCT 
CTGATGAGGT TGGAGGAAGG TCCTCCTCAC ACTAAAAACT GGAGGCCCCA GATTTTGGTG 
CTCACAACTT TGGATGGGGA GCAGAACGTG GAGCAGCCTC GACTGCTGTC CCTGACCAGT 
CAGCTGAAAG CAGGCAAAGG TTTGACCATT GTAGGAGCGT GTATAGAAGG CACCTACTTA 
AACAACCAGC CAAAGACTCA GAAAGCAGAT CAGTCCCTAA GGAAGCTGAT GGAGGTTGAG 
AAGGTGAAGG GATTCAGTCA AGTGGTGATC TCTTCTAACC TGCGGGATGC CACCTCACAC 
CTAATTCAAG CTGGTGGTCT GGGTGGGCTA CGCCACAATA CTGTTCTGGT CAGCTTTCCC 
AAGAACTGGA AACAGGCGGA GGAGCACCAC CGTTGCAGGA ACTTTATTGA GGTTGTTAGG 
GAGACCACAG CAGGTCACAT GGCCTTACTG GTCCCGAAGA ACATTTCTGC ATATCCATCA 
AATGGAGAAC GTTTTACCGA GGGCCACATT GACGTGTGGT GGATTGTCCA CGATGGAGGC 
ATGCTCATGC TCCTTCCCTT TCTTCTCCGC CAGCATAAGG TTTGGAGGAA GTGCAAGATG 
CGTATCTTCA CTGTGGCTCA GATGGATGAC AACAGCATTC AGATGAAGAA AGACCTGATG 
ACGTTCCTTT ATCACCTGCG TCTTGATGCT GCAGTTGAAG TGGTTGAAAT GCTTGACAAT 
GACATCTCAG CTTACACCTA CGAAAAGACC CTGATGATGG AGCAGCGATC GCAAATCCTC 
AAGGAGATGC ATCTAACCAA GAATGAGCGG GAGAGAGAGA TTCAGAGCAT CACAGATGTG 
TCCCGTGGCT CTATTAGGCG TAAGAACCCA TCAAACCTGC ACCCCCAGAG GAGTATCGCT 
GAGGAGTCTG TGGCAGGAAG TGGTGAAGAA AAGCCTGAAG AGGAGGTTCA GCTCATTCAG 
GGAAAAAATG CCACCCCCAC GCCCACTAGC CCCACCTCCC CTACTGCTCC CACACCAGCA 
ACTATGTGCC CTGGAGAGGA GATTCAGATG ACCTGGACGG ACGATACGGA GAAGGCCAAT 
AACCCAGCAG TGGCCAATCC TGAAAACATA AAGGACATGT TCAACATGAA ACCGGAATGG 
GAGAACCTGA ATCAGTCCAA TGTGAGACGC ATGCACCATG CGCAGAAGCT CAATGAGGTC 
ATTGTGAAGA AATCGCAAGA AGCTAAACTA GTCCTGCTAA ACATGCCTGG ACCACCTAGG 
AACCGCACTG GAGAGGAGAA CTACATGGAG TTCCTCGAGG TCTTGACTGA AGGTCTCAAC 
AGGGTCCTTC TCGTGCGTGG AGGTGGACGT GAAGTCATTA CCATCTACTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A5B ( XM_009304148.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009302423.1 Danio rerio solute carrier family 12 (potassium/chloride transporter), member 5b (slc12a5b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa47178
Clone ID Related Accession (Same CDS sequence)	XM_009304148.3 , XM_009304148.2
Accession Version	XM_009304148.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC797331 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304148.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAACA ACCTCAATGA ATGTGAAGAA GGCGATGGGG GACCCAACAG CCAGGGAGAT 
GCAACATCCA AGGAAAGCAG CCCTTTCATC AACAGCAGCA CCAGTGACGT AGAAAAAAGC 
CAGCAGTATG ATGGGAAAAA CATGGCTCTG TTTGAGGAGG AGATGGACAC TAGTCCCATG 
GTATCTTCTC TGCTGAGCAG TCTTGCCAAC TACTCCAACC TGACACAGGG CAGTAAAGAG 
CATGAAGAGG CAGAAAACAA TGAGGAGGCA CGCAAGAAAG CTGCACAGGC TCCTCGTATG 
GGCACAATAA TGGGCGTTTA CCTACCATGT CTTCAAAACA TCCTTGGGGT GATTTTGTTC 
CTCAGGATGA CCTGGCTAGT GGGTGTTGGA GGCGTCCTGG GAACCTTCAC CATCGTCTTC 
ATGTGCTGCT CCACGACTAT GCTGACTGCT ATCTCAATGA GTGCTATTGC CACCAATGGA 
GTTGTGCCAG CTGGAGGTTC GTACTACATG ATCTCTCGAT CACTCGGACC GGAGTTTGGA 
GGAGCTGTTG GCATTTGTTT CTTCTTGGGC ACCACTTTTG CTGGAGCCAT GTACATCTTG 
GGGTGCATCG AAATCCTGCT GATCTACATT GTTCCATCAG CTGCCATATT TAAAATGGAG 
GGTCTTGAGG GGTCAGAAGC CGAGGCAGCG CTGTTGAATA ACATGCGCGT GTACGGTACC 
ATCGTTCTCA CCTTCATGGC TATAGTCGTC TTTGTTGGTG TGAAGTATGT CAACAAGTTG 
GCTCTTGTGT TCCTCGCCTG CGTCATTCTC TCCATCCTGG CTATCTACGC GGGGGTCATC 
AAGACTTCTT TTGATCCACC TGACTTTCCG GTGTGTGTGC TTGGGAACCG AACTCTTGTG 
TCAAAGGCGT ATGACATTTG TGCCAAGACC ATAGAGCGGG GGAATGCCAC CATCACTACC 
AAGCTTTGGA GATCCTTCTG TGACTCTGAG TTCCTGAACG CCACCTGTGA TGAGTACTTT 
GTTAACAACA ATATCAGTCA GATCCAGGGA ATCCCTGGAG TCACCAGTGG CATCCTGGCA 
GAAAACCTTT TCAGTGGCTA TATGGAGAAA AATTCTGTCT TAGAAAAGAG AGGTCTACAG 
GCAGTACAGG ACCCTGAACT CCCAGTAACC AACAGTAACC GATATGTGTT GGCTGATATC 
ACCAGTTTCT TCACCCTGCT GGTTGGAATC TACTTCCCCT CTGTCACAGG TATCATGGCA 
GGTTCCAACC GTTCTGGTGA CCTGCAGGAT GCTCAGAAGT CCATCCCTGT TGGTACCATC 
TTGGCCATCA CAACCACCTC CATCATCTAC ATGTCTAGTG TAATTCTGTT TGGAGCTTGT 
GTGGATGGAG TTGTTTTGAG GGACAAATTT GGAGAAGGTG TCAGTGGTAA CCTGGTTATT 
GGCACGCTGG CATGGCCCTC ACCCTGGGTC ATTGTGTTTG GCTCCTTCTT CTCCACCTGT 
GGGGCCGGGC TTCAAAGTTT GACAGGAGCA CCCCGCCTCC TTCAAGCTAT TGCCCGAGAT 
GGCATCATTC CTTTCCTCAG GGTGTTTGGT CATGGCAAAG CTAACGGTGA ACCTACATGG 
GCTCTCCTTC TGACAGCCTG CATCTGTGAA AGTGGCATCC TCATTGCCTC CCTGGATGCA 
GTCGCACCTA TTCTGTCCAT GTTCTTTCTC ATGTGTTACA TGTTTGTAAA CCTGGCCTGT 
GCACTGCAAA CTCTCCTCAG AACCCCAAAC TGGAGACCAC GCTTTAAGTT TTACCATTGG 
GCTTTGTCAC TCTTGGGCAT GAGCTTGTGT CTTACCCTGA TGTTCCTGTG CTCCTGGTAC 
TATGCTATTG TTGCCATGGT AATCGCCGGC TGCATCTACA AATACATTGA ATTCTGCGGA 
GCAGAGAAAG AATGGGGTGA TGGAATCCGT GGTATCTCTT TGAGCGCTGC TCGTTTTGCT 
CTGATGAGGT TGGAGGAAGG TCCTCCTCAC ACTAAAAACT GGAGGCCCCA GATTTTGGTG 
CTCACAACTT TGGATGGAGA GCAGAACGTG GAGCAGCCTC GACTGCTGTC CCTGACCAGT 
CAGCTGAAAG CAGGCAAAGG TTTGACCATT GTAGGAGCGT GTATAGAAGG CACCTACTTA 
AACAACCAGC CAAAGACTCA GAAAGCAGAT CAGTCCCTAA GGAAGCTGAT GGAGGTTGAG 
AAGGTGAAGG GATTCAGTCA AGTGGTGATC TCTTCTAACC TGCGGGATGC CACCTCACAC 
CTAATTCAAG CTGGTGGTCT GGGTGGGCTA CGCCACAATA CTGTTCTGGT CAGCTTTCCC 
AAGAACTGGA AACAGGCTGA GGAGCACCAC CGTTGCAGGA ACTTCATTGA GGTTGTTAGG 
GAGACCACAG CAGGTCACAT GGCCTTACTG GTCCCGAAGA ACATTTCTGC ATATCCATCA 
AATGGAGAGC GTTTCACCGA GGGCCACATT GACGTGTGGT GGATTGTCCA CGATGGAGGC 
ATGCTCATGC TCCTTCCCTT TCTTCTCCGC CAGCATAAGG TTTGGAGGAA GTGCAAGATG 
CGTATCTTCA CTGTGGCTCA GATGGATGAC AACAGCATTC AGATGAAGAA AGACCTGATG 
ACGTTCCTTT ATCACCTGCG TCTTGATGCT GCAGTTGAAG TGGTTGAAAT GCTTGACAAT 
GACATCTCAG CTTACACCTA CGAAAAGACC CTGATGATGG AGCAGCGATC GCAAATCCTC 
AAGGAGATGC ATCTAACCAA GAATGAGCGG GAGAGAGAGA TTCAGAGCAT CACAGATGTG 
TCCCGTGGCT CTATTAGGCG TAAGAACCCA TCAAACCTGC ACCCCCAGAG GAGTATCGCT 
GAGGAGTCGG TGGCAGGAAG TGGTGAAGAA AAGCCTGAAG AGGAGTCTGT CCGTGTCTCC 
CATCCTCGAC AGGTTCAGCT CATTCAGGGA AAAAATGCCA CCCCCACGCC CACTAGCCCC 
ACCTCCCCTA CTGCTCCCAC ACCAGCGACT ATGAGCCCTG GAGAGGAGAT TCAGATGACC 
TGGACGGACG ATACGGAGAA GGCCAATAAC CCAGCAGTGG CCAATCCTGA AAACATAAAG 
GACATGTTCA ACATGAAACC GGAATGGGAG AACCTGAATC AGTCCAATGT GAGACGCATG 
CACCATGCGC AGAAGCTCAA TGAGGTCATT GTGAAGAAAT CGCAAGAAGC TAAACTAGTC 
CTGCTAAACA TGCCTGGACC ACCTAGGAAC CGCACTGGAG AGGAGAACTA CATGGAGTTC 
CTCGAGGTCT TGACTGAAGG TCTCAACAGG GTCCTTCTTG TGCGTGGAGG TGGACGTGAA 
GTCATTACCA TCTACTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302423.1
CDS	206..3586
Translation	MMNNLNECEEGDGGPNSQGDATSKESSPFINSSTSDVEKSQQYDGKNMALFEEEMDTSPMVSSLLSSLANYSNLTQGSKEHEEAENNEEARKKAAQAPRMGTIMGVYLPCLQNILGVILFLRMTWLVGVGGVLGTFTIVFMCCSTTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGICFFLGTTFAGAMYILGCIEILLIYIVPSAAIFKMEGLEGSEAEAALLNNMRVYGTIVLTFMAIVVFVGVKYVNKLALVFLACVILSILAIYAGVIKTSFDPPDFPVCVLGNRTLVSKAYDICAKTIERGNATITTKLWRSFCDSEFLNATCDEYFVNNNISQIQGIPGVTSGILAENLFSGYMEKNSVLEKRGLQAVQDPELPVTNSNRYVLADITSFFTLLVGIYFPSVTGIMAGSNRSGDLQDAQKSIPVGTILAITTTSIIYMSSVILFGACVDGVVLRDKFGEGVSGNLVIGTLAWPSPWVIVFGSFFSTCGAGLQSLTGAPRLLQAIARDGIIPFLRVFGHGKANGEPTWALLLTACICESGILIASLDAVAPILSMFFLMCYMFVNLACALQTLLRTPNWRPRFKFYHWALSLLGMSLCLTLMFLCSWYYAIVAMVIAGCIYKYIEFCGAEKEWGDGIRGISLSAARFALMRLEEGPPHTKNWRPQILVLTTLDGEQNVEQPRLLSLTSQLKAGKGLTIVGACIEGTYLNNQPKTQKADQSLRKLMEVEKVKGFSQVVISSNLRDATSHLIQAGGLGGLRHNTVLVSFPKNWKQAEEHHRCRNFIEVVRETTAGHMALLVPKNISAYPSNGERFTEGHIDVWWIVHDGGMLMLLPFLLRQHKVWRKCKMRIFTVAQMDDNSIQMKKDLMTFLYHLRLDAAVEVVEMLDNDISAYTYEKTLMMEQRSQILKEMHLTKNEREREIQSITDVSRGSIRRKNPSNLHPQRSIAEESVAGSGEEKPEEESVRVSHPRQVQLIQGKNATPTPTSPTSPTAPTPATMSPGEEIQMTWTDDTEKANNPAVANPENIKDMFNMKPEWENLNQSNVRRMHHAQKLNEVIVKKSQEAKLVLLNMPGPPRNRTGEENYMEFLEVLTEGLNRVLLVRGGGREVITIYS

Target ORF information:

RefSeq Version	XM_009304148.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio solute carrier family 12 (potassium/chloride transporter), member 5b (slc12a5b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304148.3

ORF Insert Sequence:

ATGATGAACA ACCTCAATGA ATGTGAAGAA GGCGATGGGG GACCCAACAG CCAGGGAGAT 
GCAACATCCA AGGAAAGCAG CCCTTTCATC AACAGCAGCA CCAGTGACGT AGAAAAAAGC 
CAGCAGTATG ATGGGAAAAA CATGGCTCTG TTTGAGGAGG AGATGGACAC TAGTCCCATG 
GTATCTTCTC TGCTGAGCAG TCTTGCCAAC TACTCCAACC TGACACAGGG CAGTAAAGAG 
CATGAAGAGG CAGAAAACAA TGAGGAGGCA CGCAAGAAAG CTGCACAGGC TCCTCGTATG 
GGCACAATAA TGGGCGTTTA CCTACCATGT CTTCAAAACA TCCTTGGGGT GATTTTGTTC 
CTCAGGATGA CCTGGCTAGT GGGTGTTGGA GGCGTCCTGG GAACCTTCAC CATCGTCTTC 
ATGTGCTGCT CCACGACTAT GCTGACTGCT ATCTCAATGA GTGCTATTGC CACCAATGGA 
GTTGTGCCAG CTGGAGGTTC GTACTACATG ATCTCTCGAT CACTCGGACC GGAGTTTGGA 
GGAGCTGTTG GCATTTGTTT CTTCTTGGGC ACCACTTTTG CTGGAGCCAT GTACATCTTG 
GGGTGCATCG AAATCCTGCT GATCTACATT GTTCCATCAG CTGCCATATT TAAAATGGAG 
GGTCTTGAGG GGTCAGAAGC CGAGGCAGCG CTGTTGAATA ACATGCGCGT GTACGGTACC 
ATCGTTCTCA CCTTCATGGC TATAGTCGTC TTTGTTGGTG TGAAGTATGT CAACAAGTTG 
GCTCTTGTGT TCCTCGCCTG CGTCATTCTC TCCATCCTGG CTATCTACGC GGGGGTCATC 
AAGACTTCTT TTGATCCACC TGACTTTCCG GTGTGTGTGC TTGGGAACCG AACTCTTGTG 
TCAAAGGCGT ATGACATTTG TGCCAAGACC ATAGAGCGGG GGAATGCCAC CATCACTACC 
AAGCTTTGGA GATCCTTCTG TGACTCTGAG TTCCTGAACG CCACCTGTGA TGAGTACTTT 
GTTAACAACA ATATCAGTCA GATCCAGGGA ATCCCTGGAG TCACCAGTGG CATCCTGGCA 
GAAAACCTTT TCAGTGGCTA TATGGAGAAA AATTCTGTCT TAGAAAAGAG AGGTCTACAG 
GCAGTACAGG ACCCTGAACT CCCAGTAACC AACAGTAACC GATATGTGTT GGCTGATATC 
ACCAGTTTCT TCACCCTGCT GGTTGGAATC TACTTCCCCT CTGTCACAGG TATCATGGCA 
GGTTCCAACC GTTCTGGTGA CCTGCAGGAT GCTCAGAAGT CCATCCCTGT TGGTACCATC 
TTGGCCATCA CAACCACCTC CATCATCTAC ATGTCTAGTG TAATTCTGTT TGGAGCTTGT 
GTGGATGGAG TTGTTTTGAG GGACAAATTT GGAGAAGGTG TCAGTGGTAA CCTGGTTATT 
GGCACGCTGG CATGGCCCTC ACCCTGGGTC ATTGTGTTTG GCTCCTTCTT CTCCACCTGT 
GGGGCCGGGC TTCAAAGTTT GACAGGAGCA CCCCGCCTCC TTCAAGCTAT TGCCCGAGAT 
GGCATCATTC CTTTCCTCAG GGTGTTTGGT CATGGCAAAG CTAACGGTGA ACCTACATGG 
GCTCTCCTTC TGACAGCCTG CATCTGTGAA AGTGGCATCC TCATTGCCTC CCTGGATGCA 
GTCGCACCTA TTCTGTCCAT GTTCTTTCTC ATGTGTTACA TGTTTGTAAA CCTGGCCTGT 
GCACTGCAAA CTCTCCTCAG AACCCCAAAC TGGAGACCAC GCTTTAAGTT TTACCATTGG 
GCTTTGTCAC TCTTGGGCAT GAGCTTGTGT CTTACCCTGA TGTTCCTGTG CTCCTGGTAC 
TATGCTATTG TTGCCATGGT AATCGCCGGC TGCATCTACA AATACATTGA ATTCTGCGGA 
GCAGAGAAAG AATGGGGTGA TGGAATCCGT GGTATCTCTT TGAGCGCTGC TCGTTTTGCT 
CTGATGAGGT TGGAGGAAGG TCCTCCTCAC ACTAAAAACT GGAGGCCCCA GATTTTGGTG 
CTCACAACTT TGGATGGAGA GCAGAACGTG GAGCAGCCTC GACTGCTGTC CCTGACCAGT 
CAGCTGAAAG CAGGCAAAGG TTTGACCATT GTAGGAGCGT GTATAGAAGG CACCTACTTA 
AACAACCAGC CAAAGACTCA GAAAGCAGAT CAGTCCCTAA GGAAGCTGAT GGAGGTTGAG 
AAGGTGAAGG GATTCAGTCA AGTGGTGATC TCTTCTAACC TGCGGGATGC CACCTCACAC 
CTAATTCAAG CTGGTGGTCT GGGTGGGCTA CGCCACAATA CTGTTCTGGT CAGCTTTCCC 
AAGAACTGGA AACAGGCTGA GGAGCACCAC CGTTGCAGGA ACTTCATTGA GGTTGTTAGG 
GAGACCACAG CAGGTCACAT GGCCTTACTG GTCCCGAAGA ACATTTCTGC ATATCCATCA 
AATGGAGAGC GTTTCACCGA GGGCCACATT GACGTGTGGT GGATTGTCCA CGATGGAGGC 
ATGCTCATGC TCCTTCCCTT TCTTCTCCGC CAGCATAAGG TTTGGAGGAA GTGCAAGATG 
CGTATCTTCA CTGTGGCTCA GATGGATGAC AACAGCATTC AGATGAAGAA AGACCTGATG 
ACGTTCCTTT ATCACCTGCG TCTTGATGCT GCAGTTGAAG TGGTTGAAAT GCTTGACAAT 
GACATCTCAG CTTACACCTA CGAAAAGACC CTGATGATGG AGCAGCGATC GCAAATCCTC 
AAGGAGATGC ATCTAACCAA GAATGAGCGG GAGAGAGAGA TTCAGAGCAT CACAGATGTG 
TCCCGTGGCT CTATTAGGCG TAAGAACCCA TCAAACCTGC ACCCCCAGAG GAGTATCGCT 
GAGGAGTCGG TGGCAGGAAG TGGTGAAGAA AAGCCTGAAG AGGAGTCTGT CCGTGTCTCC 
CATCCTCGAC AGGTTCAGCT CATTCAGGGA AAAAATGCCA CCCCCACGCC CACTAGCCCC 
ACCTCCCCTA CTGCTCCCAC ACCAGCGACT ATGAGCCCTG GAGAGGAGAT TCAGATGACC 
TGGACGGACG ATACGGAGAA GGCCAATAAC CCAGCAGTGG CCAATCCTGA AAACATAAAG 
GACATGTTCA ACATGAAACC GGAATGGGAG AACCTGAATC AGTCCAATGT GAGACGCATG 
CACCATGCGC AGAAGCTCAA TGAGGTCATT GTGAAGAAAT CGCAAGAAGC TAAACTAGTC 
CTGCTAAACA TGCCTGGACC ACCTAGGAAC CGCACTGGAG AGGAGAACTA CATGGAGTTC 
CTCGAGGTCT TGACTGAAGG TCTCAACAGG GTCCTTCTTG TGCGTGGAGG TGGACGTGAA 
GTCATTACCA TCTACTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A5B ( XM_009304148.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009302423.1 Danio rerio solute carrier family 12 (potassium/chloride transporter), member 5b (slc12a5b), transcript variant X1, mRNA.

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CloneID	ODa47178
Clone ID Related Accession (Same CDS sequence)	XM_009304148.3 , XM_009304148.2
Accession Version	XM_009304148.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein LOC797331 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304148.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAACA ACCTCAATGA ATGTGAAGAA GGCGATGGGG GACCCAACAG CCAGGGAGAT 
GCAACATCCA AGGAAAGCAG CCCTTTCATC AACAGCAGCA CCAGTGACGT AGAAAAAAGC 
CAGCAGTATG ATGGGAAAAA CATGGCTCTG TTTGAGGAGG AGATGGACAC TAGTCCCATG 
GTATCTTCTC TGCTGAGCAG TCTTGCCAAC TACTCCAACC TGACACAGGG CAGTAAAGAG 
CATGAAGAGG CAGAAAACAA TGAGGAGGCA CGCAAGAAAG CTGCACAGGC TCCTCGTATG 
GGCACAATAA TGGGCGTTTA CCTACCATGT CTTCAAAACA TCCTTGGGGT GATTTTGTTC 
CTCAGGATGA CCTGGCTAGT GGGTGTTGGA GGCGTCCTGG GAACCTTCAC CATCGTCTTC 
ATGTGCTGCT CCACGACTAT GCTGACTGCT ATCTCAATGA GTGCTATTGC CACCAATGGA 
GTTGTGCCAG CTGGAGGTTC GTACTACATG ATCTCTCGAT CACTCGGACC GGAGTTTGGA 
GGAGCTGTTG GCATTTGTTT CTTCTTGGGC ACCACTTTTG CTGGAGCCAT GTACATCTTG 
GGGTGCATCG AAATCCTGCT GATCTACATT GTTCCATCAG CTGCCATATT TAAAATGGAG 
GGTCTTGAGG GGTCAGAAGC CGAGGCAGCG CTGTTGAATA ACATGCGCGT GTACGGTACC 
ATCGTTCTCA CCTTCATGGC TATAGTCGTC TTTGTTGGTG TGAAGTATGT CAACAAGTTG 
GCTCTTGTGT TCCTCGCCTG CGTCATTCTC TCCATCCTGG CTATCTACGC GGGGGTCATC 
AAGACTTCTT TTGATCCACC TGACTTTCCG GTGTGTGTGC TTGGGAACCG AACTCTTGTG 
TCAAAGGCGT ATGACATTTG TGCCAAGACC ATAGAGCGGG GGAATGCCAC CATCACTACC 
AAGCTTTGGA GATCCTTCTG TGACTCTGAG TTCCTGAACG CCACCTGTGA TGAGTACTTT 
GTTAACAACA ATATCAGTCA GATCCAGGGA ATCCCTGGAG TCACCAGTGG CATCCTGGCA 
GAAAACCTTT TCAGTGGCTA TATGGAGAAA AATTCTGTCT TAGAAAAGAG AGGTCTACAG 
GCAGTACAGG ACCCTGAACT CCCAGTAACC AACAGTAACC GATATGTGTT GGCTGATATC 
ACCAGTTTCT TCACCCTGCT GGTTGGAATC TACTTCCCCT CTGTCACAGG TATCATGGCA 
GGTTCCAACC GTTCTGGTGA CCTGCAGGAT GCTCAGAAGT CCATCCCTGT TGGTACCATC 
TTGGCCATCA CAACCACCTC CATCATCTAC ATGTCTAGTG TAATTCTGTT TGGAGCTTGT 
GTGGATGGAG TTGTTTTGAG GGACAAATTT GGAGAAGGTG TCAGTGGTAA CCTGGTTATT 
GGCACGCTGG CATGGCCCTC ACCCTGGGTC ATTGTGTTTG GCTCCTTCTT CTCCACCTGT 
GGGGCCGGGC TTCAAAGTTT GACAGGAGCA CCCCGCCTCC TTCAAGCTAT TGCCCGAGAT 
GGCATCATTC CTTTCCTCAG GGTGTTTGGT CATGGCAAAG CTAACGGTGA ACCTACATGG 
GCTCTCCTTC TGACAGCCTG CATCTGTGAA AGTGGCATCC TCATTGCCTC CCTGGATGCA 
GTCGCACCTA TTCTGTCCAT GTTCTTTCTC ATGTGTTACA TGTTTGTAAA CCTGGCCTGT 
GCACTGCAAA CTCTCCTCAG AACCCCAAAC TGGAGACCAC GCTTTAAGTT TTACCATTGG 
GCTTTGTCAC TCTTGGGCAT GAGCTTGTGT CTTACCCTGA TGTTCCTGTG CTCCTGGTAC 
TATGCTATTG TTGCCATGGT AATCGCCGGC TGCATCTACA AATACATTGA ATTCTGCGGA 
GCAGAGAAAG AATGGGGTGA TGGAATCCGT GGTATCTCTT TGAGCGCTGC TCGTTTTGCT 
CTGATGAGGT TGGAGGAAGG TCCTCCTCAC ACTAAAAACT GGAGGCCCCA GATTTTGGTG 
CTCACAACTT TGGATGGAGA GCAGAACGTG GAGCAGCCTC GACTGCTGTC CCTGACCAGT 
CAGCTGAAAG CAGGCAAAGG TTTGACCATT GTAGGAGCGT GTATAGAAGG CACCTACTTA 
AACAACCAGC CAAAGACTCA GAAAGCAGAT CAGTCCCTAA GGAAGCTGAT GGAGGTTGAG 
AAGGTGAAGG GATTCAGTCA AGTGGTGATC TCTTCTAACC TGCGGGATGC CACCTCACAC 
CTAATTCAAG CTGGTGGTCT GGGTGGGCTA CGCCACAATA CTGTTCTGGT CAGCTTTCCC 
AAGAACTGGA AACAGGCTGA GGAGCACCAC CGTTGCAGGA ACTTCATTGA GGTTGTTAGG 
GAGACCACAG CAGGTCACAT GGCCTTACTG GTCCCGAAGA ACATTTCTGC ATATCCATCA 
AATGGAGAGC GTTTCACCGA GGGCCACATT GACGTGTGGT GGATTGTCCA CGATGGAGGC 
ATGCTCATGC TCCTTCCCTT TCTTCTCCGC CAGCATAAGG TTTGGAGGAA GTGCAAGATG 
CGTATCTTCA CTGTGGCTCA GATGGATGAC AACAGCATTC AGATGAAGAA AGACCTGATG 
ACGTTCCTTT ATCACCTGCG TCTTGATGCT GCAGTTGAAG TGGTTGAAAT GCTTGACAAT 
GACATCTCAG CTTACACCTA CGAAAAGACC CTGATGATGG AGCAGCGATC GCAAATCCTC 
AAGGAGATGC ATCTAACCAA GAATGAGCGG GAGAGAGAGA TTCAGAGCAT CACAGATGTG 
TCCCGTGGCT CTATTAGGCG TAAGAACCCA TCAAACCTGC ACCCCCAGAG GAGTATCGCT 
GAGGAGTCGG TGGCAGGAAG TGGTGAAGAA AAGCCTGAAG AGGAGTCTGT CCGTGTCTCC 
CATCCTCGAC AGGTTCAGCT CATTCAGGGA AAAAATGCCA CCCCCACGCC CACTAGCCCC 
ACCTCCCCTA CTGCTCCCAC ACCAGCGACT ATGAGCCCTG GAGAGGAGAT TCAGATGACC 
TGGACGGACG ATACGGAGAA GGCCAATAAC CCAGCAGTGG CCAATCCTGA AAACATAAAG 
GACATGTTCA ACATGAAACC GGAATGGGAG AACCTGAATC AGTCCAATGT GAGACGCATG 
CACCATGCGC AGAAGCTCAA TGAGGTCATT GTGAAGAAAT CGCAAGAAGC TAAACTAGTC 
CTGCTAAACA TGCCTGGACC ACCTAGGAAC CGCACTGGAG AGGAGAACTA CATGGAGTTC 
CTCGAGGTCT TGACTGAAGG TCTCAACAGG GTCCTTCTTG TGCGTGGAGG TGGACGTGAA 
GTCATTACCA TCTACTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302423.1
CDS	243..3623
Translation	MMNNLNECEEGDGGPNSQGDATSKESSPFINSSTSDVEKSQQYDGKNMALFEEEMDTSPMVSSLLSSLANYSNLTQGSKEHEEAENNEEARKKAAQAPRMGTIMGVYLPCLQNILGVILFLRMTWLVGVGGVLGTFTIVFMCCSTTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGICFFLGTTFAGAMYILGCIEILLIYIVPSAAIFKMEGLEGSEAEAALLNNMRVYGTIVLTFMAIVVFVGVKYVNKLALVFLACVILSILAIYAGVIKTSFDPPDFPVCVLGNRTLVSKAYDICAKTIERGNATITTKLWRSFCDSEFLNATCDEYFVNNNISQIQGIPGVTSGILAENLFSGYMEKNSVLEKRGLQAVQDPELPVTNSNRYVLADITSFFTLLVGIYFPSVTGIMAGSNRSGDLQDAQKSIPVGTILAITTTSIIYMSSVILFGACVDGVVLRDKFGEGVSGNLVIGTLAWPSPWVIVFGSFFSTCGAGLQSLTGAPRLLQAIARDGIIPFLRVFGHGKANGEPTWALLLTACICESGILIASLDAVAPILSMFFLMCYMFVNLACALQTLLRTPNWRPRFKFYHWALSLLGMSLCLTLMFLCSWYYAIVAMVIAGCIYKYIEFCGAEKEWGDGIRGISLSAARFALMRLEEGPPHTKNWRPQILVLTTLDGEQNVEQPRLLSLTSQLKAGKGLTIVGACIEGTYLNNQPKTQKADQSLRKLMEVEKVKGFSQVVISSNLRDATSHLIQAGGLGGLRHNTVLVSFPKNWKQAEEHHRCRNFIEVVRETTAGHMALLVPKNISAYPSNGERFTEGHIDVWWIVHDGGMLMLLPFLLRQHKVWRKCKMRIFTVAQMDDNSIQMKKDLMTFLYHLRLDAAVEVVEMLDNDISAYTYEKTLMMEQRSQILKEMHLTKNEREREIQSITDVSRGSIRRKNPSNLHPQRSIAEESVAGSGEEKPEEESVRVSHPRQVQLIQGKNATPTPTSPTSPTAPTPATMSPGEEIQMTWTDDTEKANNPAVANPENIKDMFNMKPEWENLNQSNVRRMHHAQKLNEVIVKKSQEAKLVLLNMPGPPRNRTGEENYMEFLEVLTEGLNRVLLVRGGGREVITIYS

Target ORF information:

RefSeq Version	XM_009304148.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio solute carrier family 12 (potassium/chloride transporter), member 5b (slc12a5b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304148.2

ORF Insert Sequence:

ATGATGAACA ACCTCAATGA ATGTGAAGAA GGCGATGGGG GACCCAACAG CCAGGGAGAT 
GCAACATCCA AGGAAAGCAG CCCTTTCATC AACAGCAGCA CCAGTGACGT AGAAAAAAGC 
CAGCAGTATG ATGGGAAAAA CATGGCTCTG TTTGAGGAGG AGATGGACAC TAGTCCCATG 
GTATCTTCTC TGCTGAGCAG TCTTGCCAAC TACTCCAACC TGACACAGGG CAGTAAAGAG 
CATGAAGAGG CAGAAAACAA TGAGGAGGCA CGCAAGAAAG CTGCACAGGC TCCTCGTATG 
GGCACAATAA TGGGCGTTTA CCTACCATGT CTTCAAAACA TCCTTGGGGT GATTTTGTTC 
CTCAGGATGA CCTGGCTAGT GGGTGTTGGA GGCGTCCTGG GAACCTTCAC CATCGTCTTC 
ATGTGCTGCT CCACGACTAT GCTGACTGCT ATCTCAATGA GTGCTATTGC CACCAATGGA 
GTTGTGCCAG CTGGAGGTTC GTACTACATG ATCTCTCGAT CACTCGGACC GGAGTTTGGA 
GGAGCTGTTG GCATTTGTTT CTTCTTGGGC ACCACTTTTG CTGGAGCCAT GTACATCTTG 
GGGTGCATCG AAATCCTGCT GATCTACATT GTTCCATCAG CTGCCATATT TAAAATGGAG 
GGTCTTGAGG GGTCAGAAGC CGAGGCAGCG CTGTTGAATA ACATGCGCGT GTACGGTACC 
ATCGTTCTCA CCTTCATGGC TATAGTCGTC TTTGTTGGTG TGAAGTATGT CAACAAGTTG 
GCTCTTGTGT TCCTCGCCTG CGTCATTCTC TCCATCCTGG CTATCTACGC GGGGGTCATC 
AAGACTTCTT TTGATCCACC TGACTTTCCG GTGTGTGTGC TTGGGAACCG AACTCTTGTG 
TCAAAGGCGT ATGACATTTG TGCCAAGACC ATAGAGCGGG GGAATGCCAC CATCACTACC 
AAGCTTTGGA GATCCTTCTG TGACTCTGAG TTCCTGAACG CCACCTGTGA TGAGTACTTT 
GTTAACAACA ATATCAGTCA GATCCAGGGA ATCCCTGGAG TCACCAGTGG CATCCTGGCA 
GAAAACCTTT TCAGTGGCTA TATGGAGAAA AATTCTGTCT TAGAAAAGAG AGGTCTACAG 
GCAGTACAGG ACCCTGAACT CCCAGTAACC AACAGTAACC GATATGTGTT GGCTGATATC 
ACCAGTTTCT TCACCCTGCT GGTTGGAATC TACTTCCCCT CTGTCACAGG TATCATGGCA 
GGTTCCAACC GTTCTGGTGA CCTGCAGGAT GCTCAGAAGT CCATCCCTGT TGGTACCATC 
TTGGCCATCA CAACCACCTC CATCATCTAC ATGTCTAGTG TAATTCTGTT TGGAGCTTGT 
GTGGATGGAG TTGTTTTGAG GGACAAATTT GGAGAAGGTG TCAGTGGTAA CCTGGTTATT 
GGCACGCTGG CATGGCCCTC ACCCTGGGTC ATTGTGTTTG GCTCCTTCTT CTCCACCTGT 
GGGGCCGGGC TTCAAAGTTT GACAGGAGCA CCCCGCCTCC TTCAAGCTAT TGCCCGAGAT 
GGCATCATTC CTTTCCTCAG GGTGTTTGGT CATGGCAAAG CTAACGGTGA ACCTACATGG 
GCTCTCCTTC TGACAGCCTG CATCTGTGAA AGTGGCATCC TCATTGCCTC CCTGGATGCA 
GTCGCACCTA TTCTGTCCAT GTTCTTTCTC ATGTGTTACA TGTTTGTAAA CCTGGCCTGT 
GCACTGCAAA CTCTCCTCAG AACCCCAAAC TGGAGACCAC GCTTTAAGTT TTACCATTGG 
GCTTTGTCAC TCTTGGGCAT GAGCTTGTGT CTTACCCTGA TGTTCCTGTG CTCCTGGTAC 
TATGCTATTG TTGCCATGGT AATCGCCGGC TGCATCTACA AATACATTGA ATTCTGCGGA 
GCAGAGAAAG AATGGGGTGA TGGAATCCGT GGTATCTCTT TGAGCGCTGC TCGTTTTGCT 
CTGATGAGGT TGGAGGAAGG TCCTCCTCAC ACTAAAAACT GGAGGCCCCA GATTTTGGTG 
CTCACAACTT TGGATGGAGA GCAGAACGTG GAGCAGCCTC GACTGCTGTC CCTGACCAGT 
CAGCTGAAAG CAGGCAAAGG TTTGACCATT GTAGGAGCGT GTATAGAAGG CACCTACTTA 
AACAACCAGC CAAAGACTCA GAAAGCAGAT CAGTCCCTAA GGAAGCTGAT GGAGGTTGAG 
AAGGTGAAGG GATTCAGTCA AGTGGTGATC TCTTCTAACC TGCGGGATGC CACCTCACAC 
CTAATTCAAG CTGGTGGTCT GGGTGGGCTA CGCCACAATA CTGTTCTGGT CAGCTTTCCC 
AAGAACTGGA AACAGGCTGA GGAGCACCAC CGTTGCAGGA ACTTCATTGA GGTTGTTAGG 
GAGACCACAG CAGGTCACAT GGCCTTACTG GTCCCGAAGA ACATTTCTGC ATATCCATCA 
AATGGAGAGC GTTTCACCGA GGGCCACATT GACGTGTGGT GGATTGTCCA CGATGGAGGC 
ATGCTCATGC TCCTTCCCTT TCTTCTCCGC CAGCATAAGG TTTGGAGGAA GTGCAAGATG 
CGTATCTTCA CTGTGGCTCA GATGGATGAC AACAGCATTC AGATGAAGAA AGACCTGATG 
ACGTTCCTTT ATCACCTGCG TCTTGATGCT GCAGTTGAAG TGGTTGAAAT GCTTGACAAT 
GACATCTCAG CTTACACCTA CGAAAAGACC CTGATGATGG AGCAGCGATC GCAAATCCTC 
AAGGAGATGC ATCTAACCAA GAATGAGCGG GAGAGAGAGA TTCAGAGCAT CACAGATGTG 
TCCCGTGGCT CTATTAGGCG TAAGAACCCA TCAAACCTGC ACCCCCAGAG GAGTATCGCT 
GAGGAGTCGG TGGCAGGAAG TGGTGAAGAA AAGCCTGAAG AGGAGTCTGT CCGTGTCTCC 
CATCCTCGAC AGGTTCAGCT CATTCAGGGA AAAAATGCCA CCCCCACGCC CACTAGCCCC 
ACCTCCCCTA CTGCTCCCAC ACCAGCGACT ATGAGCCCTG GAGAGGAGAT TCAGATGACC 
TGGACGGACG ATACGGAGAA GGCCAATAAC CCAGCAGTGG CCAATCCTGA AAACATAAAG 
GACATGTTCA ACATGAAACC GGAATGGGAG AACCTGAATC AGTCCAATGT GAGACGCATG 
CACCATGCGC AGAAGCTCAA TGAGGTCATT GTGAAGAAAT CGCAAGAAGC TAAACTAGTC 
CTGCTAAACA TGCCTGGACC ACCTAGGAAC CGCACTGGAG AGGAGAACTA CATGGAGTTC 
CTCGAGGTCT TGACTGAAGG TCTCAACAGG GTCCTTCTTG TGCGTGGAGG TGGACGTGAA 
GTCATTACCA TCTACTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

γ-Aminobutyric acid receptor alpha 1 subunit loss of function causes genetic generalized epilepsy by impairing inhibitory network neurodevelopment.
Epilepsia59(11)2061-2074(2018 11)
Samarut É,Swaminathan A,Riché R,Liao M,Hassan-Abdi R,Renault S,Allard M,Dufour L,Cossette P,Soussi-Yanicostas N,Drapeau P

Non-canonical mTOR-Independent Role of DEPDC5 in Regulating GABAergic Network Development.
Current biology : CB28(12)1924-1937.e5(2018 06)
Swaminathan A,Hassan-Abdi R,Renault S,Siekierska A,Riché R,Liao M,de Witte PAM,Yanicostas C,Soussi-Yanicostas N,Drapeau P,Samarut É

Neurotransmitter-mediated activity spatially controls neuronal migration in the zebrafish cerebellum.
PLoS biology16(1)e2002226(2018 01)
Theisen U,Hennig C,Ring T,Schnabel R,Köster RW

Glycine is able to induce both a motility speed in- and decrease during zebrafish neuronal migration.
Communicative & integrative biology11(3)1-7(2018)
Theisen U,Hey S,Hennig CD,Schnabel R,Köster RW

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 03)
Bayés À,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

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SLC12A5B cDNA ORF clone, Danio rerio(Zebrafish)

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