SMURF1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SMURF1
Entrez Gene ID	57154
Full Name	SMAD specific E3 ubiquitin protein ligase 1
General protein information	Preferred Names SMAD specific E3 ubiquitin protein ligase 1 Names E3 ubiquitin-protein ligase SMURF1 E3 ubiquitin ligase SMURF1 HECT-type E3 ubiquitin transferase SMURF1 Smad ubiquitination regulatory factor 1 Smad-specific E3 ubiquitin ligase 1 hSMURF1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q22.1
Summary	This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Dec 2010].
Disorder MIM:	605568

mRNA and Protein(s)

mRNA	Protein	Name
NM_020429.3	NP_065162.1	E3 ubiquitin-protein ligase SMURF1 isoform 1
NM_020429.2	NP_065162.1	E3 ubiquitin-protein ligase SMURF1 isoform 1
NM_181349.3	NP_851994.1	E3 ubiquitin-protein ligase SMURF1 isoform 2
NM_181349.2	NP_851994.1	E3 ubiquitin-protein ligase SMURF1 isoform 2
NM_001199847.1	NP_001186776.1	E3 ubiquitin-protein ligase SMURF1 isoform 3
XM_017012457.1	XP_016867946.1	E3 ubiquitin-protein ligase SMURF1 isoform X1
NM_001199847.2	NP_001186776.1	E3 ubiquitin-protein ligase SMURF1 isoform 3

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	SMURF1	XP_414794.3
Homo sapiens (human)	SMURF1	NP_065162.1
Macaca mulatta (Rhesus monkey)	SMURF1	XP_002803251.1
Canis lupus familiaris (dog)	SMURF1	XP_005621234.1
Xenopus tropicalis (tropical clawed frog)	LOC100491582	XP_004917897.1
Pan troglodytes (chimpanzee)	SMURF1	XP_528043.3
Bos taurus (cattle)	SMURF1	XP_002698191.3
Mus musculus (house mouse)	Smurf1	NP_001033716.1
Rattus norvegicus (Norway rat)	Smurf1	NP_001103068.1
Danio rerio (zebrafish)	smurf1	NP_001001943.1

Gene Ontology
Bibliography

Related articles in PubMed

Upregulation of microRNA‑194‑5p inhibits hypopharyngeal carcinoma cell proliferation, migration and invasion by targeting SMURF1 via the mTOR signaling pathway.
Xu S, Hui L, Yang N, Wang Y, Zhao N, Jiang XJ
International journal of oncology54(4)1245-1255(2019 Apr)

Hippo kinase NDR2 inhibits IL-17 signaling by promoting Smurf1-mediated MEKK2 ubiquitination and degradation.
Ma X, Wang D, Li N, Gao P, Zhang M, Zhang Y
Molecular immunology105131-136(2019 01)

SMAD specific E3 ubiquitin protein ligase 1 promotes ovarian cancer cell migration and invasion via the activation of the RhoA/ROCK signaling pathway.
Wang W, Du H, Liu H, Hu F, Liu G
Oncology reports41(1)668-676(2019 Jan)

IRAK2 counterbalances oncogenic Smurf1 in colon cancer cells by dictating ER stress.
Liu J, Chen Y, Huang Q, Liu W, Ji X, Hu F, Zhu Y, Zhang L, Dong G
Cellular signalling4869-80(2018 Aug)

The E3 ubiquitin ligase SMURF1 regulates cell-fate specification and outflow tract septation during mammalian heart development.
Koefoed K, Skat-Rørdam J, Andersen P, Warzecha CB, Pye M, Andersen TA, Ajbro KD, Bendsen E, Narimatsu M, Vilhardt F, Pedersen LB, Wrana JL, Anderson RH, Møllgård K, Christensen ST, Larsen LA
Scientific reports8(1)9542(2018 06)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SMURF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMURF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu06128	NM_001199847.2 Latest version!	Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu55910	NM_181349.3 Latest version!	Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu97406	XM_017012457.1 Latest version!	Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu55935	NM_020429.3 Latest version!	Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$426.30 $609.00
View Old Accession Versions >>
OHu55935	NM_020429.2	Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$426.30 $609.00
OHu55910	NM_181349.2	Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu06128	NM_001199847.1	Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMURF1 ( NM_001199847.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186776.1 Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu06128
Clone ID Related Accession (Same CDS sequence)	NM_001199847.1 , NM_001199847.2
Accession Version	NM_001199847.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549036800000
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase SMURF1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB727777.1, BC144414.1, BC136804.1, BC152468.1 and BM981719.1. Transcript Variant: This variant (3) lacks an in-frame exon in the coding region and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. Isoform 3 is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.45337.1, BC136804.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA 
AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG 
TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA 
GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA 
ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC 
CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC 
CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA 
CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC 
GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC 
ACGTGGCACG ACCCCAGGAT ACCAAGAGAC CTTAACAGTG TGAACTGTGA TGAACTTGGA 
CCACTGCCGC CAGGCTGGGA AGTCAGAAGT ACAGTTTCTG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCAAGGTTAC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CCACTGCCCA GTGAGGGCTC TCTGGAGGAC 
GAGGAGCTTC CTGCCCAGAG ATACGAAAGA GATCTAGTCC AGAAGCTGAA AGTCCTCAGA 
CACGAACTGT CGCTTCAGCA GCCCCAAGCT GGTCATTGCC GCATCGAAGT GTCCAGAGAA 
GAAATCTTTG AGGAGTCTTA CCGCCAGATA ATGAAGATGC GACCGAAAGA CTTGAAAAAA 
CGGCTGATGG TGAAATTCCG TGGGGAAGAA GGTTTGGATT ACGGTGGTGT GGCCAGGGAG 
TGGCTTTACT TGCTGTGCCA TGAAATGCTG AATCCTTATT ACGGGCTCTT CCAGTATTCT 
ACGGACAATA TTTACATGTT GCAAATAAAT CCGGATTCTT CAATCAACCC CGACCACTTG 
TCTTATTTCC ACTTTGTGGG GCGGATCATG GGGCTGGCTG TGTTCCATGG ACACTACATC 
AACGGGGGCT TCACAGTGCC CTTCTACAAG CAGCTGCTGG GGAAGCCCAT CCAGCTCTCA 
GATCTGGAAT CTGTGGACCC AGAGCTGCAT AAGAGCTTGG TGTGGATCCT AGAGAACGAC 
ATCACGCCTG TACTGGACCA CACCTTCTGC GTGGAACACA ACGCCTTCGG GCGGATCCTG 
CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCAGTCA CAGAGGAGAA TAAGAAAGAA 
TACGTCCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCT 
CTGCAGAAGG GGTTCAATGA GCTCATCCCT CAACATCTGC TGAAGCCTTT TGACCAGAAG 
GAACTGGAGC TGATCATAGG CGGCCTGGAT AAAATAGACT TGAACGACTG GAAGTCGAAC 
ACGCGGCTGA AGCACTGTGT GGCCGACAGC AACATCGTGC GGTGGTTCTG GCAAGCGGTG 
GAGACGTTCG ATGAAGAAAG GAGGGCCAGG CTCCTGCAGT TTGTGACTGG GTCCACGCGA 
GTCCCGCTCC AAGGCTTCAA GGCTTTGCAA GGCGCGGCAG GGCCCCGGCT GTTCACCATC 
CACCTGATAG ACGCGAACAC AGACAACCTT CCGAAGGCCC ATACCTGCTT TAACCGGATC 
GACATTCCAC CATATGAGTC CTATGAGAAG CTCTACGAGA AGCTGCTGAC AGCCGTGGAG 
GAGACCTGCG GGTTTGCTGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186776.1
CDS	341..2527
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLENEGTVYEDSGPGRPLSCFMEEPAPYTDSTGAAAGGGNCRFVESPSQDQRLQAQRLRNPDVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLPLPSEGSLEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	NM_001199847.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199847.1

ORF Insert Sequence:

ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA 
AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG 
TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA 
GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA 
ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC 
CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC 
CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA 
CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC 
GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC 
ACGTGGCACG ACCCCAGGAT ACCAAGAGAC CTTAACAGTG TGAACTGTGA TGAACTTGGA 
CCACTGCCGC CAGGCTGGGA AGTCAGAAGT ACAGTTTCTG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCAAGGTTAC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CCACTGCCCA GTGAGGGCTC TCTGGAGGAC 
GAGGAGCTTC CTGCCCAGAG ATACGAAAGA GATCTAGTCC AGAAGCTGAA AGTCCTCAGA 
CACGAACTGT CGCTTCAGCA GCCCCAAGCT GGTCATTGCC GCATCGAAGT GTCCAGAGAA 
GAAATCTTTG AGGAGTCTTA CCGCCAGATA ATGAAGATGC GACCGAAAGA CTTGAAAAAA 
CGGCTGATGG TGAAATTCCG TGGGGAAGAA GGTTTGGATT ACGGTGGTGT GGCCAGGGAG 
TGGCTTTACT TGCTGTGCCA TGAAATGCTG AATCCTTATT ACGGGCTCTT CCAGTATTCT 
ACGGACAATA TTTACATGTT GCAAATAAAT CCGGATTCTT CAATCAACCC CGACCACTTG 
TCTTATTTCC ACTTTGTGGG GCGGATCATG GGGCTGGCTG TGTTCCATGG ACACTACATC 
AACGGGGGCT TCACAGTGCC CTTCTACAAG CAGCTGCTGG GGAAGCCCAT CCAGCTCTCA 
GATCTGGAAT CTGTGGACCC AGAGCTGCAT AAGAGCTTGG TGTGGATCCT AGAGAACGAC 
ATCACGCCTG TACTGGACCA CACCTTCTGC GTGGAACACA ACGCCTTCGG GCGGATCCTG 
CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCAGTCA CAGAGGAGAA TAAGAAAGAA 
TACGTCCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCT 
CTGCAGAAGG GGTTCAATGA GCTCATCCCT CAACATCTGC TGAAGCCTTT TGACCAGAAG 
GAACTGGAGC TGATCATAGG CGGCCTGGAT AAAATAGACT TGAACGACTG GAAGTCGAAC 
ACGCGGCTGA AGCACTGTGT GGCCGACAGC AACATCGTGC GGTGGTTCTG GCAAGCGGTG 
GAGACGTTCG ATGAAGAAAG GAGGGCCAGG CTCCTGCAGT TTGTGACTGG GTCCACGCGA 
GTCCCGCTCC AAGGCTTCAA GGCTTTGCAA GGCGCGGCAG GGCCCCGGCT GTTCACCATC 
CACCTGATAG ACGCGAACAC AGACAACCTT CCGAAGGCCC ATACCTGCTT TAACCGGATC 
GACATTCCAC CATATGAGTC CTATGAGAAG CTCTACGAGA AGCTGCTGAC AGCCGTGGAG 
GAGACCTGCG GGTTTGCTGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF1 ( NM_001199847.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186776.1 Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu06128
Clone ID Related Accession (Same CDS sequence)	NM_001199847.1 , NM_001199847.2
Accession Version	NM_001199847.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1574006400000
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase SMURF1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB727777.1, BC144414.1, BC136804.1 and BC152468.1. On Jun 2, 2019 this sequence version replaced NM_001199847.1. Transcript Variant: This variant (3) lacks an in-frame exon in the coding region and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. Isoform 3 is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.45337.1, BC136804.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA 
AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG 
TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA 
GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA 
ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC 
CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC 
CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA 
CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC 
GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC 
ACGTGGCACG ACCCCAGGAT ACCAAGAGAC CTTAACAGTG TGAACTGTGA TGAACTTGGA 
CCACTGCCGC CAGGCTGGGA AGTCAGAAGT ACAGTTTCTG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCAAGGTTAC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CCACTGCCCA GTGAGGGCTC TCTGGAGGAC 
GAGGAGCTTC CTGCCCAGAG ATACGAAAGA GATCTAGTCC AGAAGCTGAA AGTCCTCAGA 
CACGAACTGT CGCTTCAGCA GCCCCAAGCT GGTCATTGCC GCATCGAAGT GTCCAGAGAA 
GAAATCTTTG AGGAGTCTTA CCGCCAGATA ATGAAGATGC GACCGAAAGA CTTGAAAAAA 
CGGCTGATGG TGAAATTCCG TGGGGAAGAA GGTTTGGATT ACGGTGGTGT GGCCAGGGAG 
TGGCTTTACT TGCTGTGCCA TGAAATGCTG AATCCTTATT ACGGGCTCTT CCAGTATTCT 
ACGGACAATA TTTACATGTT GCAAATAAAT CCGGATTCTT CAATCAACCC CGACCACTTG 
TCTTATTTCC ACTTTGTGGG GCGGATCATG GGGCTGGCTG TGTTCCATGG ACACTACATC 
AACGGGGGCT TCACAGTGCC CTTCTACAAG CAGCTGCTGG GGAAGCCCAT CCAGCTCTCA 
GATCTGGAAT CTGTGGACCC AGAGCTGCAT AAGAGCTTGG TGTGGATCCT AGAGAACGAC 
ATCACGCCTG TACTGGACCA CACCTTCTGC GTGGAACACA ACGCCTTCGG GCGGATCCTG 
CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCAGTCA CAGAGGAGAA TAAGAAAGAA 
TACGTCCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCT 
CTGCAGAAGG GGTTCAATGA GCTCATCCCT CAACATCTGC TGAAGCCTTT TGACCAGAAG 
GAACTGGAGC TGATCATAGG CGGCCTGGAT AAAATAGACT TGAACGACTG GAAGTCGAAC 
ACGCGGCTGA AGCACTGTGT GGCCGACAGC AACATCGTGC GGTGGTTCTG GCAAGCGGTG 
GAGACGTTCG ATGAAGAAAG GAGGGCCAGG CTCCTGCAGT TTGTGACTGG GTCCACGCGA 
GTCCCGCTCC AAGGCTTCAA GGCTTTGCAA GGCGCGGCAG GGCCCCGGCT GTTCACCATC 
CACCTGATAG ACGCGAACAC AGACAACCTT CCGAAGGCCC ATACCTGCTT TAACCGGATC 
GACATTCCAC CATATGAGTC CTATGAGAAG CTCTACGAGA AGCTGCTGAC AGCCGTGGAG 
GAGACCTGCG GGTTTGCTGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186776.1
CDS	329..2515
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLENEGTVYEDSGPGRPLSCFMEEPAPYTDSTGAAAGGGNCRFVESPSQDQRLQAQRLRNPDVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLPLPSEGSLEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	NM_001199847.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199847.2

ORF Insert Sequence:

ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA 
AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG 
TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA 
GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA 
ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC 
CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC 
CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA 
CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC 
GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC 
ACGTGGCACG ACCCCAGGAT ACCAAGAGAC CTTAACAGTG TGAACTGTGA TGAACTTGGA 
CCACTGCCGC CAGGCTGGGA AGTCAGAAGT ACAGTTTCTG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCAAGGTTAC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CCACTGCCCA GTGAGGGCTC TCTGGAGGAC 
GAGGAGCTTC CTGCCCAGAG ATACGAAAGA GATCTAGTCC AGAAGCTGAA AGTCCTCAGA 
CACGAACTGT CGCTTCAGCA GCCCCAAGCT GGTCATTGCC GCATCGAAGT GTCCAGAGAA 
GAAATCTTTG AGGAGTCTTA CCGCCAGATA ATGAAGATGC GACCGAAAGA CTTGAAAAAA 
CGGCTGATGG TGAAATTCCG TGGGGAAGAA GGTTTGGATT ACGGTGGTGT GGCCAGGGAG 
TGGCTTTACT TGCTGTGCCA TGAAATGCTG AATCCTTATT ACGGGCTCTT CCAGTATTCT 
ACGGACAATA TTTACATGTT GCAAATAAAT CCGGATTCTT CAATCAACCC CGACCACTTG 
TCTTATTTCC ACTTTGTGGG GCGGATCATG GGGCTGGCTG TGTTCCATGG ACACTACATC 
AACGGGGGCT TCACAGTGCC CTTCTACAAG CAGCTGCTGG GGAAGCCCAT CCAGCTCTCA 
GATCTGGAAT CTGTGGACCC AGAGCTGCAT AAGAGCTTGG TGTGGATCCT AGAGAACGAC 
ATCACGCCTG TACTGGACCA CACCTTCTGC GTGGAACACA ACGCCTTCGG GCGGATCCTG 
CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCAGTCA CAGAGGAGAA TAAGAAAGAA 
TACGTCCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCT 
CTGCAGAAGG GGTTCAATGA GCTCATCCCT CAACATCTGC TGAAGCCTTT TGACCAGAAG 
GAACTGGAGC TGATCATAGG CGGCCTGGAT AAAATAGACT TGAACGACTG GAAGTCGAAC 
ACGCGGCTGA AGCACTGTGT GGCCGACAGC AACATCGTGC GGTGGTTCTG GCAAGCGGTG 
GAGACGTTCG ATGAAGAAAG GAGGGCCAGG CTCCTGCAGT TTGTGACTGG GTCCACGCGA 
GTCCCGCTCC AAGGCTTCAA GGCTTTGCAA GGCGCGGCAG GGCCCCGGCT GTTCACCATC 
CACCTGATAG ACGCGAACAC AGACAACCTT CCGAAGGCCC ATACCTGCTT TAACCGGATC 
GACATTCCAC CATATGAGTC CTATGAGAAG CTCTACGAGA AGCTGCTGAC AGCCGTGGAG 
GAGACCTGCG GGTTTGCTGT GGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF1 ( NM_181349.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_851994.1 Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu55910
Clone ID Related Accession (Same CDS sequence)	NM_181349.3 , NM_181349.2
Accession Version	NM_181349.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1574006400000
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase SMURF1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB727777.1, AF199364.2 and BC152468.1. On Jun 2, 2019 this sequence version replaced NM_181349.2. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. Isoform 2 is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF199364.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA 
AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG 
TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA 
GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA 
ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC 
CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC 
CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA 
CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC 
GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC 
ACGTGGCACG ACCCCAGGAT ACCAAGAGAC CTTAACAGTG TGAACTGTGA TGAACTTGGA 
CCACTGCCGC CAGGCTGGGA AGTCAGAAGT ACAGTTTCTG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCAAGGTTAC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CCACTGCCCA GTGAGGGCTC TCTGGAGGAC 
GAGGAGCTTC CTGCCCAGAG ATACGAAAGA GATCTAGTCC AGAAGCTGAA AGTCCTCAGA 
CACGAACTGT CGCTTCAGCA GCCCCAAGCT GGTCATTGCC GCATCGAAGT GTCCAGAGAA 
GAAATCTTTG AGGAGTCTTA CCGCCAGATA ATGAAGATGC GACCGAAAGA CTTGAAAAAA 
CGGCTGATGG TGAAATTCCG TGGGGAAGAA GGTTTGGATT ACGGTGGTGT GGCCAGGGAG 
TGGCTTTACT TGCTGTGCCA TGAAATGCTG AATCCTTATT ACGGGCTCTT CCAGTATTCT 
ACGGACAATA TTTACATGTT GCAAATAAAT CCGGATTCTT CAATCAACCC CGACCACTTG 
TCTTATTTCC ACTTTGTGGG GCGGATCATG GGGCTGGCTG TGTTCCATGG ACACTACATC 
AACGGGGGCT TCACAGTGCC CTTCTACAAG CAGCTGCTGG GGAAGCCCAT CCAGCTCTCA 
GATCTGGAAT CTGTGGACCC AGAGCTGCAT AAGAGCTTGG TGTGGATCCT AGAGAACGAC 
ATCACGCCTG TACTGGACCA CACCTTCTGC GTGGAACACA ACGCCTTCGG GCGGATCCTG 
CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCAGTCA CAGAGGAGAA TAAGAAAGAA 
TACGTCCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCT 
CTGCAGAAGG GGTTCAATGA GCTCATCCCT CAACATCTGC TGAAGCCTTT TGACCAGAAG 
GAACTGGAGC TGATCATAGG CGGCCTGGAT AAAATAGACT TGAACGACTG GAAGTCGAAC 
ACGCGGCTGA AGCACTGTGT GGCCGACAGC AACATCGTGC GGTGGTTCTG GCAAGCGGTG 
GAGACGTTCG ATGAAGAAAG GAGGGCCAGG CTCCTGCAGT TTGTGACTGG GTCCACGCGA 
GTCCCGCTCC AAGGCTTCAA GGCTTTGCAA GGTTCTACAG GCGCGGCAGG GCCCCGGCTG 
TTCACCATCC ACCTGATAGA CGCGAACACA GACAACCTTC CGAAGGCCCA TACCTGCTTT 
AACCGGATCG ACATTCCACC ATATGAGTCC TATGAGAAGC TCTACGAGAA GCTGCTGACA 
GCCGTGGAGG AGACCTGCGG GTTTGCTGTG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_851994.1
CDS	329..2524
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLENEGTVYEDSGPGRPLSCFMEEPAPYTDSTGAAAGGGNCRFVESPSQDQRLQAQRLRNPDVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLPLPSEGSLEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	NM_181349.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181349.3

ORF Insert Sequence:

ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA 
AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG 
TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA 
GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA 
ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC 
CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC 
CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA 
CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC 
GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC 
ACGTGGCACG ACCCCAGGAT ACCAAGAGAC CTTAACAGTG TGAACTGTGA TGAACTTGGA 
CCACTGCCGC CAGGCTGGGA AGTCAGAAGT ACAGTTTCTG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCAAGGTTAC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CCACTGCCCA GTGAGGGCTC TCTGGAGGAC 
GAGGAGCTTC CTGCCCAGAG ATACGAAAGA GATCTAGTCC AGAAGCTGAA AGTCCTCAGA 
CACGAACTGT CGCTTCAGCA GCCCCAAGCT GGTCATTGCC GCATCGAAGT GTCCAGAGAA 
GAAATCTTTG AGGAGTCTTA CCGCCAGATA ATGAAGATGC GACCGAAAGA CTTGAAAAAA 
CGGCTGATGG TGAAATTCCG TGGGGAAGAA GGTTTGGATT ACGGTGGTGT GGCCAGGGAG 
TGGCTTTACT TGCTGTGCCA TGAAATGCTG AATCCTTATT ACGGGCTCTT CCAGTATTCT 
ACGGACAATA TTTACATGTT GCAAATAAAT CCGGATTCTT CAATCAACCC CGACCACTTG 
TCTTATTTCC ACTTTGTGGG GCGGATCATG GGGCTGGCTG TGTTCCATGG ACACTACATC 
AACGGGGGCT TCACAGTGCC CTTCTACAAG CAGCTGCTGG GGAAGCCCAT CCAGCTCTCA 
GATCTGGAAT CTGTGGACCC AGAGCTGCAT AAGAGCTTGG TGTGGATCCT AGAGAACGAC 
ATCACGCCTG TACTGGACCA CACCTTCTGC GTGGAACACA ACGCCTTCGG GCGGATCCTG 
CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCAGTCA CAGAGGAGAA TAAGAAAGAA 
TACGTCCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCT 
CTGCAGAAGG GGTTCAATGA GCTCATCCCT CAACATCTGC TGAAGCCTTT TGACCAGAAG 
GAACTGGAGC TGATCATAGG CGGCCTGGAT AAAATAGACT TGAACGACTG GAAGTCGAAC 
ACGCGGCTGA AGCACTGTGT GGCCGACAGC AACATCGTGC GGTGGTTCTG GCAAGCGGTG 
GAGACGTTCG ATGAAGAAAG GAGGGCCAGG CTCCTGCAGT TTGTGACTGG GTCCACGCGA 
GTCCCGCTCC AAGGCTTCAA GGCTTTGCAA GGTTCTACAG GCGCGGCAGG GCCCCGGCTG 
TTCACCATCC ACCTGATAGA CGCGAACACA GACAACCTTC CGAAGGCCCA TACCTGCTTT 
AACCGGATCG ACATTCCACC ATATGAGTCC TATGAGAAGC TCTACGAGAA GCTGCTGACA 
GCCGTGGAGG AGACCTGCGG GTTTGCTGTG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF1 ( NM_181349.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_851994.1 Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu55910
Clone ID Related Accession (Same CDS sequence)	NM_181349.3 , NM_181349.2
Accession Version	NM_181349.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549036800000
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase SMURF1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB727777.1, AF199364.2, BC152468.1 and BM981719.1. On Dec 23, 2010 this sequence version replaced NM_181349.1. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. Isoform 2 is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF199364.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA 
AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG 
TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA 
GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA 
ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC 
CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC 
CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA 
CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC 
GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC 
ACGTGGCACG ACCCCAGGAT ACCAAGAGAC CTTAACAGTG TGAACTGTGA TGAACTTGGA 
CCACTGCCGC CAGGCTGGGA AGTCAGAAGT ACAGTTTCTG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCAAGGTTAC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CCACTGCCCA GTGAGGGCTC TCTGGAGGAC 
GAGGAGCTTC CTGCCCAGAG ATACGAAAGA GATCTAGTCC AGAAGCTGAA AGTCCTCAGA 
CACGAACTGT CGCTTCAGCA GCCCCAAGCT GGTCATTGCC GCATCGAAGT GTCCAGAGAA 
GAAATCTTTG AGGAGTCTTA CCGCCAGATA ATGAAGATGC GACCGAAAGA CTTGAAAAAA 
CGGCTGATGG TGAAATTCCG TGGGGAAGAA GGTTTGGATT ACGGTGGTGT GGCCAGGGAG 
TGGCTTTACT TGCTGTGCCA TGAAATGCTG AATCCTTATT ACGGGCTCTT CCAGTATTCT 
ACGGACAATA TTTACATGTT GCAAATAAAT CCGGATTCTT CAATCAACCC CGACCACTTG 
TCTTATTTCC ACTTTGTGGG GCGGATCATG GGGCTGGCTG TGTTCCATGG ACACTACATC 
AACGGGGGCT TCACAGTGCC CTTCTACAAG CAGCTGCTGG GGAAGCCCAT CCAGCTCTCA 
GATCTGGAAT CTGTGGACCC AGAGCTGCAT AAGAGCTTGG TGTGGATCCT AGAGAACGAC 
ATCACGCCTG TACTGGACCA CACCTTCTGC GTGGAACACA ACGCCTTCGG GCGGATCCTG 
CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCAGTCA CAGAGGAGAA TAAGAAAGAA 
TACGTCCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCT 
CTGCAGAAGG GGTTCAATGA GCTCATCCCT CAACATCTGC TGAAGCCTTT TGACCAGAAG 
GAACTGGAGC TGATCATAGG CGGCCTGGAT AAAATAGACT TGAACGACTG GAAGTCGAAC 
ACGCGGCTGA AGCACTGTGT GGCCGACAGC AACATCGTGC GGTGGTTCTG GCAAGCGGTG 
GAGACGTTCG ATGAAGAAAG GAGGGCCAGG CTCCTGCAGT TTGTGACTGG GTCCACGCGA 
GTCCCGCTCC AAGGCTTCAA GGCTTTGCAA GGTTCTACAG GCGCGGCAGG GCCCCGGCTG 
TTCACCATCC ACCTGATAGA CGCGAACACA GACAACCTTC CGAAGGCCCA TACCTGCTTT 
AACCGGATCG ACATTCCACC ATATGAGTCC TATGAGAAGC TCTACGAGAA GCTGCTGACA 
GCCGTGGAGG AGACCTGCGG GTTTGCTGTG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_851994.1
CDS	341..2536
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLENEGTVYEDSGPGRPLSCFMEEPAPYTDSTGAAAGGGNCRFVESPSQDQRLQAQRLRNPDVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLPLPSEGSLEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	NM_181349.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181349.2

ORF Insert Sequence:

ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA 
AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG 
TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA 
GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA 
ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC 
CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC 
CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA 
CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC 
GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC 
ACGTGGCACG ACCCCAGGAT ACCAAGAGAC CTTAACAGTG TGAACTGTGA TGAACTTGGA 
CCACTGCCGC CAGGCTGGGA AGTCAGAAGT ACAGTTTCTG GGAGGATATA TTTTGTAGAT 
CATAATAACC GAACAACCCA GTTTACAGAC CCAAGGTTAC ACCACATCAT GAATCACCAG 
TGCCAACTCA AGGAGCCCAG CCAGCCGCTG CCACTGCCCA GTGAGGGCTC TCTGGAGGAC 
GAGGAGCTTC CTGCCCAGAG ATACGAAAGA GATCTAGTCC AGAAGCTGAA AGTCCTCAGA 
CACGAACTGT CGCTTCAGCA GCCCCAAGCT GGTCATTGCC GCATCGAAGT GTCCAGAGAA 
GAAATCTTTG AGGAGTCTTA CCGCCAGATA ATGAAGATGC GACCGAAAGA CTTGAAAAAA 
CGGCTGATGG TGAAATTCCG TGGGGAAGAA GGTTTGGATT ACGGTGGTGT GGCCAGGGAG 
TGGCTTTACT TGCTGTGCCA TGAAATGCTG AATCCTTATT ACGGGCTCTT CCAGTATTCT 
ACGGACAATA TTTACATGTT GCAAATAAAT CCGGATTCTT CAATCAACCC CGACCACTTG 
TCTTATTTCC ACTTTGTGGG GCGGATCATG GGGCTGGCTG TGTTCCATGG ACACTACATC 
AACGGGGGCT TCACAGTGCC CTTCTACAAG CAGCTGCTGG GGAAGCCCAT CCAGCTCTCA 
GATCTGGAAT CTGTGGACCC AGAGCTGCAT AAGAGCTTGG TGTGGATCCT AGAGAACGAC 
ATCACGCCTG TACTGGACCA CACCTTCTGC GTGGAACACA ACGCCTTCGG GCGGATCCTG 
CAGCATGAAC TGAAACCCAA TGGCAGAAAT GTGCCAGTCA CAGAGGAGAA TAAGAAAGAA 
TACGTCCGGT TGTATGTAAA CTGGAGGTTT ATGAGAGGAA TCGAAGCCCA GTTCTTAGCT 
CTGCAGAAGG GGTTCAATGA GCTCATCCCT CAACATCTGC TGAAGCCTTT TGACCAGAAG 
GAACTGGAGC TGATCATAGG CGGCCTGGAT AAAATAGACT TGAACGACTG GAAGTCGAAC 
ACGCGGCTGA AGCACTGTGT GGCCGACAGC AACATCGTGC GGTGGTTCTG GCAAGCGGTG 
GAGACGTTCG ATGAAGAAAG GAGGGCCAGG CTCCTGCAGT TTGTGACTGG GTCCACGCGA 
GTCCCGCTCC AAGGCTTCAA GGCTTTGCAA GGTTCTACAG GCGCGGCAGG GCCCCGGCTG 
TTCACCATCC ACCTGATAGA CGCGAACACA GACAACCTTC CGAAGGCCCA TACCTGCTTT 
AACCGGATCG ACATTCCACC ATATGAGTCC TATGAGAAGC TCTACGAGAA GCTGCTGACA 
GCCGTGGAGG AGACCTGCGG GTTTGCTGTG GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF1 ( XM_017012457.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867946.1 Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu97406
Clone ID Related Accession (Same CDS sequence)	XM_017012457.1
Accession Version	XM_017012457.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase SMURF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA 
AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG 
TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA 
GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA 
ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC 
CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC 
CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA 
CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC 
GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC 
ACGTGGCACG ACCCCAGGAT ACCAAGTCCC TCGGGGACCA TTCCTGGGGG AGATGCAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCTGAGC CCAGAGACCT TAACAGTGTG 
AACTGTGATG AACTTGGACC ACTGCCGCCA GGCTGGGAAG TCAGAAGTAC AGTTTCTGGG 
AGGATATATT TTGTAGATCA TAATAACCGA ACAACCCAGT TTACAGACCC AAGGTTACAC 
CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCC ACTGCCCAGT 
GAGGGCTCTC TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ACGAAAGAGA TCTAGTCCAG 
AAGCTGAAAG TCCTCAGACA CGAACTGTCG CTTCAGCAGC CCCAAGCTGG TCATTGCCGC 
ATCGAAGTGT CCAGAGAAGA AATCTTTGAG GAGTCTTACC GCCAGATAAT GAAGATGCGA 
CCGAAAGACT TGAAAAAACG GCTGATGGTG AAATTCCGTG GGGAAGAAGG TTTGGATTAC 
GGTGGTGTGG CCAGGGAGTG GCTTTACTTG CTGTGCCATG AAATGCTGAA TCCTTATTAC 
GGGCTCTTCC AGTATTCTAC GGACAATATT TACATGTTGC AAATAAATCC GGATTCTTCA 
ATCAACCCCG ACCACTTGTC TTATTTCCAC TTTGTGGGGC GGATCATGGG GCTGGCTGTG 
TTCCATGGAC ACTACATCAA CGGGGGCTTC ACAGTGCCCT TCTACAAGCA GCTGCTGGGG 
AAGCCCATCC AGCTCTCAGA TCTGGAATCT GTGGACCCAG AGCTGCATAA GAGCTTGGTG 
TGGATCCTAG AGAACGACAT CACGCCTGTA CTGGACCACA CCTTCTGCGT GGAACACAAC 
GCCTTCGGGC GGATCCTGCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCAGTCACA 
GAGGAGAATA AGAAAGAATA CGTCCGGTTG TATGTAAACT GGAGGTTTAT GAGAGGAATC 
GAAGCCCAGT TCTTAGCTCT GCAGAAGGGG TTCAATGAGC TCATCCCTCA ACATCTGCTG 
AAGCCTTTTG ACCAGAAGGA ACTGGAGCTG ATCATAGGCG GCCTGGATAA AATAGACTTG 
AACGACTGGA AGTCGAACAC GCGGCTGAAG CACTGTGTGG CCGACAGCAA CATCGTGCGG 
TGGTTCTGGC AAGCGGTGGA GACGTTCGAT GAAGAAAGGA GGGCCAGGCT CCTGCAGTTT 
GTGACTGGGT CCACGCGAGT CCCGCTCCAA GGCTTCAAGG CTTTGCAAGG CGCGGCAGGG 
CCCCGGCTGT TCACCATCCA CCTGATAGAC GCGAACACAG ACAACCTTCC GAAGGCCCAT 
ACCTGCTTTA ACCGGATCGA CATTCCACCA TATGAGTCCT ATGAGAAGCT CTACGAGAAG 
CTGCTGACAG CCGTGGAGGA GACCTGCGGG TTTGCTGTGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867946.1
CDS	339..2603
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLENEGTVYEDSGPGRPLSCFMEEPAPYTDSTGAAAGGGNCRFVESPSQDQRLQAQRLRNPDVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPSPSGTIPGGDAAFLYEFLLQGHTSEPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLPLPSEGSLEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	XM_017012457.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012457.1

ORF Insert Sequence:

ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA 
AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG 
TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA 
GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA 
ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC 
CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC 
CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA 
CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC 
GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC 
ACGTGGCACG ACCCCAGGAT ACCAAGTCCC TCGGGGACCA TTCCTGGGGG AGATGCAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCTGAGC CCAGAGACCT TAACAGTGTG 
AACTGTGATG AACTTGGACC ACTGCCGCCA GGCTGGGAAG TCAGAAGTAC AGTTTCTGGG 
AGGATATATT TTGTAGATCA TAATAACCGA ACAACCCAGT TTACAGACCC AAGGTTACAC 
CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCC ACTGCCCAGT 
GAGGGCTCTC TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ACGAAAGAGA TCTAGTCCAG 
AAGCTGAAAG TCCTCAGACA CGAACTGTCG CTTCAGCAGC CCCAAGCTGG TCATTGCCGC 
ATCGAAGTGT CCAGAGAAGA AATCTTTGAG GAGTCTTACC GCCAGATAAT GAAGATGCGA 
CCGAAAGACT TGAAAAAACG GCTGATGGTG AAATTCCGTG GGGAAGAAGG TTTGGATTAC 
GGTGGTGTGG CCAGGGAGTG GCTTTACTTG CTGTGCCATG AAATGCTGAA TCCTTATTAC 
GGGCTCTTCC AGTATTCTAC GGACAATATT TACATGTTGC AAATAAATCC GGATTCTTCA 
ATCAACCCCG ACCACTTGTC TTATTTCCAC TTTGTGGGGC GGATCATGGG GCTGGCTGTG 
TTCCATGGAC ACTACATCAA CGGGGGCTTC ACAGTGCCCT TCTACAAGCA GCTGCTGGGG 
AAGCCCATCC AGCTCTCAGA TCTGGAATCT GTGGACCCAG AGCTGCATAA GAGCTTGGTG 
TGGATCCTAG AGAACGACAT CACGCCTGTA CTGGACCACA CCTTCTGCGT GGAACACAAC 
GCCTTCGGGC GGATCCTGCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCAGTCACA 
GAGGAGAATA AGAAAGAATA CGTCCGGTTG TATGTAAACT GGAGGTTTAT GAGAGGAATC 
GAAGCCCAGT TCTTAGCTCT GCAGAAGGGG TTCAATGAGC TCATCCCTCA ACATCTGCTG 
AAGCCTTTTG ACCAGAAGGA ACTGGAGCTG ATCATAGGCG GCCTGGATAA AATAGACTTG 
AACGACTGGA AGTCGAACAC GCGGCTGAAG CACTGTGTGG CCGACAGCAA CATCGTGCGG 
TGGTTCTGGC AAGCGGTGGA GACGTTCGAT GAAGAAAGGA GGGCCAGGCT CCTGCAGTTT 
GTGACTGGGT CCACGCGAGT CCCGCTCCAA GGCTTCAAGG CTTTGCAAGG CGCGGCAGGG 
CCCCGGCTGT TCACCATCCA CCTGATAGAC GCGAACACAG ACAACCTTCC GAAGGCCCAT 
ACCTGCTTTA ACCGGATCGA CATTCCACCA TATGAGTCCT ATGAGAAGCT CTACGAGAAG 
CTGCTGACAG CCGTGGAGGA GACCTGCGGG TTTGCTGTGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF1 ( NM_020429.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_065162.1 Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu55935
Clone ID Related Accession (Same CDS sequence)	NM_020429.3 , NM_020429.2
Accession Version	NM_020429.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1574092800000
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase SMURF1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB727777.1 and BC152468.1. On Jun 2, 2019 this sequence version replaced NM_020429.2. Transcript Variant: This variant (1) encodes the longer isoform (1) of this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC152468.1, AB046845.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA 
AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG 
TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA 
GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA 
ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC 
CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC 
CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA 
CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC 
GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC 
ACGTGGCACG ACCCCAGGAT ACCAAGTCCC TCGGGGACCA TTCCTGGGGG AGATGCAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCTGAGC CCAGAGACCT TAACAGTGTG 
AACTGTGATG AACTTGGACC ACTGCCGCCA GGCTGGGAAG TCAGAAGTAC AGTTTCTGGG 
AGGATATATT TTGTAGATCA TAATAACCGA ACAACCCAGT TTACAGACCC AAGGTTACAC 
CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCC ACTGCCCAGT 
GAGGGCTCTC TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ACGAAAGAGA TCTAGTCCAG 
AAGCTGAAAG TCCTCAGACA CGAACTGTCG CTTCAGCAGC CCCAAGCTGG TCATTGCCGC 
ATCGAAGTGT CCAGAGAAGA AATCTTTGAG GAGTCTTACC GCCAGATAAT GAAGATGCGA 
CCGAAAGACT TGAAAAAACG GCTGATGGTG AAATTCCGTG GGGAAGAAGG TTTGGATTAC 
GGTGGTGTGG CCAGGGAGTG GCTTTACTTG CTGTGCCATG AAATGCTGAA TCCTTATTAC 
GGGCTCTTCC AGTATTCTAC GGACAATATT TACATGTTGC AAATAAATCC GGATTCTTCA 
ATCAACCCCG ACCACTTGTC TTATTTCCAC TTTGTGGGGC GGATCATGGG GCTGGCTGTG 
TTCCATGGAC ACTACATCAA CGGGGGCTTC ACAGTGCCCT TCTACAAGCA GCTGCTGGGG 
AAGCCCATCC AGCTCTCAGA TCTGGAATCT GTGGACCCAG AGCTGCATAA GAGCTTGGTG 
TGGATCCTAG AGAACGACAT CACGCCTGTA CTGGACCACA CCTTCTGCGT GGAACACAAC 
GCCTTCGGGC GGATCCTGCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCAGTCACA 
GAGGAGAATA AGAAAGAATA CGTCCGGTTG TATGTAAACT GGAGGTTTAT GAGAGGAATC 
GAAGCCCAGT TCTTAGCTCT GCAGAAGGGG TTCAATGAGC TCATCCCTCA ACATCTGCTG 
AAGCCTTTTG ACCAGAAGGA ACTGGAGCTG ATCATAGGCG GCCTGGATAA AATAGACTTG 
AACGACTGGA AGTCGAACAC GCGGCTGAAG CACTGTGTGG CCGACAGCAA CATCGTGCGG 
TGGTTCTGGC AAGCGGTGGA GACGTTCGAT GAAGAAAGGA GGGCCAGGCT CCTGCAGTTT 
GTGACTGGGT CCACGCGAGT CCCGCTCCAA GGCTTCAAGG CTTTGCAAGG TTCTACAGGC 
GCGGCAGGGC CCCGGCTGTT CACCATCCAC CTGATAGACG CGAACACAGA CAACCTTCCG 
AAGGCCCATA CCTGCTTTAA CCGGATCGAC ATTCCACCAT ATGAGTCCTA TGAGAAGCTC 
TACGAGAAGC TGCTGACAGC CGTGGAGGAG ACCTGCGGGT TTGCTGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065162.1
CDS	329..2602
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLENEGTVYEDSGPGRPLSCFMEEPAPYTDSTGAAAGGGNCRFVESPSQDQRLQAQRLRNPDVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPSPSGTIPGGDAAFLYEFLLQGHTSEPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLPLPSEGSLEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	NM_020429.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020429.3

ORF Insert Sequence:

ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA 
AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG 
TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA 
GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA 
ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC 
CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC 
CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA 
CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC 
GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC 
ACGTGGCACG ACCCCAGGAT ACCAAGTCCC TCGGGGACCA TTCCTGGGGG AGATGCAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCTGAGC CCAGAGACCT TAACAGTGTG 
AACTGTGATG AACTTGGACC ACTGCCGCCA GGCTGGGAAG TCAGAAGTAC AGTTTCTGGG 
AGGATATATT TTGTAGATCA TAATAACCGA ACAACCCAGT TTACAGACCC AAGGTTACAC 
CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCC ACTGCCCAGT 
GAGGGCTCTC TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ACGAAAGAGA TCTAGTCCAG 
AAGCTGAAAG TCCTCAGACA CGAACTGTCG CTTCAGCAGC CCCAAGCTGG TCATTGCCGC 
ATCGAAGTGT CCAGAGAAGA AATCTTTGAG GAGTCTTACC GCCAGATAAT GAAGATGCGA 
CCGAAAGACT TGAAAAAACG GCTGATGGTG AAATTCCGTG GGGAAGAAGG TTTGGATTAC 
GGTGGTGTGG CCAGGGAGTG GCTTTACTTG CTGTGCCATG AAATGCTGAA TCCTTATTAC 
GGGCTCTTCC AGTATTCTAC GGACAATATT TACATGTTGC AAATAAATCC GGATTCTTCA 
ATCAACCCCG ACCACTTGTC TTATTTCCAC TTTGTGGGGC GGATCATGGG GCTGGCTGTG 
TTCCATGGAC ACTACATCAA CGGGGGCTTC ACAGTGCCCT TCTACAAGCA GCTGCTGGGG 
AAGCCCATCC AGCTCTCAGA TCTGGAATCT GTGGACCCAG AGCTGCATAA GAGCTTGGTG 
TGGATCCTAG AGAACGACAT CACGCCTGTA CTGGACCACA CCTTCTGCGT GGAACACAAC 
GCCTTCGGGC GGATCCTGCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCAGTCACA 
GAGGAGAATA AGAAAGAATA CGTCCGGTTG TATGTAAACT GGAGGTTTAT GAGAGGAATC 
GAAGCCCAGT TCTTAGCTCT GCAGAAGGGG TTCAATGAGC TCATCCCTCA ACATCTGCTG 
AAGCCTTTTG ACCAGAAGGA ACTGGAGCTG ATCATAGGCG GCCTGGATAA AATAGACTTG 
AACGACTGGA AGTCGAACAC GCGGCTGAAG CACTGTGTGG CCGACAGCAA CATCGTGCGG 
TGGTTCTGGC AAGCGGTGGA GACGTTCGAT GAAGAAAGGA GGGCCAGGCT CCTGCAGTTT 
GTGACTGGGT CCACGCGAGT CCCGCTCCAA GGCTTCAAGG CTTTGCAAGG TTCTACAGGC 
GCGGCAGGGC CCCGGCTGTT CACCATCCAC CTGATAGACG CGAACACAGA CAACCTTCCG 
AAGGCCCATA CCTGCTTTAA CCGGATCGAC ATTCCACCAT ATGAGTCCTA TGAGAAGCTC 
TACGAGAAGC TGCTGACAGC CGTGGAGGAG ACCTGCGGGT TTGCTGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMURF1 ( NM_020429.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_065162.1 Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu55935
Clone ID Related Accession (Same CDS sequence)	NM_020429.3 , NM_020429.2
Accession Version	NM_020429.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550764800000
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase SMURF1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB727777.1, BC152468.1 and BM981719.1. On Dec 23, 2010 this sequence version replaced NM_020429.1. Transcript Variant: This variant (1) encodes the longer isoform (1) of this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC152468.1, AB046845.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA 
AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG 
TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA 
GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA 
ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC 
CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC 
CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA 
CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC 
GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC 
ACGTGGCACG ACCCCAGGAT ACCAAGTCCC TCGGGGACCA TTCCTGGGGG AGATGCAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCTGAGC CCAGAGACCT TAACAGTGTG 
AACTGTGATG AACTTGGACC ACTGCCGCCA GGCTGGGAAG TCAGAAGTAC AGTTTCTGGG 
AGGATATATT TTGTAGATCA TAATAACCGA ACAACCCAGT TTACAGACCC AAGGTTACAC 
CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCC ACTGCCCAGT 
GAGGGCTCTC TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ACGAAAGAGA TCTAGTCCAG 
AAGCTGAAAG TCCTCAGACA CGAACTGTCG CTTCAGCAGC CCCAAGCTGG TCATTGCCGC 
ATCGAAGTGT CCAGAGAAGA AATCTTTGAG GAGTCTTACC GCCAGATAAT GAAGATGCGA 
CCGAAAGACT TGAAAAAACG GCTGATGGTG AAATTCCGTG GGGAAGAAGG TTTGGATTAC 
GGTGGTGTGG CCAGGGAGTG GCTTTACTTG CTGTGCCATG AAATGCTGAA TCCTTATTAC 
GGGCTCTTCC AGTATTCTAC GGACAATATT TACATGTTGC AAATAAATCC GGATTCTTCA 
ATCAACCCCG ACCACTTGTC TTATTTCCAC TTTGTGGGGC GGATCATGGG GCTGGCTGTG 
TTCCATGGAC ACTACATCAA CGGGGGCTTC ACAGTGCCCT TCTACAAGCA GCTGCTGGGG 
AAGCCCATCC AGCTCTCAGA TCTGGAATCT GTGGACCCAG AGCTGCATAA GAGCTTGGTG 
TGGATCCTAG AGAACGACAT CACGCCTGTA CTGGACCACA CCTTCTGCGT GGAACACAAC 
GCCTTCGGGC GGATCCTGCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCAGTCACA 
GAGGAGAATA AGAAAGAATA CGTCCGGTTG TATGTAAACT GGAGGTTTAT GAGAGGAATC 
GAAGCCCAGT TCTTAGCTCT GCAGAAGGGG TTCAATGAGC TCATCCCTCA ACATCTGCTG 
AAGCCTTTTG ACCAGAAGGA ACTGGAGCTG ATCATAGGCG GCCTGGATAA AATAGACTTG 
AACGACTGGA AGTCGAACAC GCGGCTGAAG CACTGTGTGG CCGACAGCAA CATCGTGCGG 
TGGTTCTGGC AAGCGGTGGA GACGTTCGAT GAAGAAAGGA GGGCCAGGCT CCTGCAGTTT 
GTGACTGGGT CCACGCGAGT CCCGCTCCAA GGCTTCAAGG CTTTGCAAGG TTCTACAGGC 
GCGGCAGGGC CCCGGCTGTT CACCATCCAC CTGATAGACG CGAACACAGA CAACCTTCCG 
AAGGCCCATA CCTGCTTTAA CCGGATCGAC ATTCCACCAT ATGAGTCCTA TGAGAAGCTC 
TACGAGAAGC TGCTGACAGC CGTGGAGGAG ACCTGCGGGT TTGCTGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065162.1
CDS	341..2614
Translation	MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLENEGTVYEDSGPGRPLSCFMEEPAPYTDSTGAAAGGGNCRFVESPSQDQRLQAQRLRNPDVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPSPSGTIPGGDAAFLYEFLLQGHTSEPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLPLPSEGSLEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE

Target ORF information:

RefSeq Version	NM_020429.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020429.2

ORF Insert Sequence:

ATGTCGAACC CCGGGACACG CAGGAACGGC TCCAGCATCA AGATCCGTCT GACAGTGTTA 
TGTGCCAAGA ACCTTGCAAA GAAAGACTTC TTCAGGCTCC CTGACCCTTT TGCAAAGATT 
GTCGTGGATG GGTCTGGGCA GTGCCACTCA ACCGACACTG TGAAAAACAC ATTGGACCCA 
AAGTGGAACC AGCACTATGA TCTATATGTT GGGAAAACGG ATTCGATAAC CATTAGCGTG 
TGGAACCATA AGAAAATTCA CAAGAAACAG GGAGCTGGCT TCCTGGGCTG TGTGCGGCTG 
CTCTCCAATG CCATCAGCAG ATTAAAAGAT ACCGGATACC AGCGTTTGGA TCTATGCAAA 
CTAAACCCCT CAGATACTGA TGCAGTTCGT GGCCAGATAG TGGTCAGTTT ACAGACACGA 
GACAGAATAG GAACCGGCGG CTCGGTGGTG GACTGCAGAG GACTGTTAGA AAATGAAGGA 
ACGGTGTATG AAGACTCCGG GCCTGGGAGG CCGCTCAGCT GCTTCATGGA GGAACCAGCC 
CCTTACACAG ATAGCACCGG TGCTGCTGCT GGAGGAGGGA ATTGCAGGTT CGTGGAGTCC 
CCAAGTCAAG ATCAAAGACT TCAGGCACAG CGGCTTCGAA ACCCTGATGT GCGAGGTTCA 
CTACAGACGC CCCAGAACCG ACCACACGGC CACCAGTCCC CGGAACTGCC CGAAGGCTAC 
GAACAAAGAA CAACAGTCCA GGGCCAAGTT TACTTTTTGC ATACACAGAC TGGAGTTAGC 
ACGTGGCACG ACCCCAGGAT ACCAAGTCCC TCGGGGACCA TTCCTGGGGG AGATGCAGCT 
TTTCTATACG AATTCCTTCT ACAAGGCCAT ACATCTGAGC CCAGAGACCT TAACAGTGTG 
AACTGTGATG AACTTGGACC ACTGCCGCCA GGCTGGGAAG TCAGAAGTAC AGTTTCTGGG 
AGGATATATT TTGTAGATCA TAATAACCGA ACAACCCAGT TTACAGACCC AAGGTTACAC 
CACATCATGA ATCACCAGTG CCAACTCAAG GAGCCCAGCC AGCCGCTGCC ACTGCCCAGT 
GAGGGCTCTC TGGAGGACGA GGAGCTTCCT GCCCAGAGAT ACGAAAGAGA TCTAGTCCAG 
AAGCTGAAAG TCCTCAGACA CGAACTGTCG CTTCAGCAGC CCCAAGCTGG TCATTGCCGC 
ATCGAAGTGT CCAGAGAAGA AATCTTTGAG GAGTCTTACC GCCAGATAAT GAAGATGCGA 
CCGAAAGACT TGAAAAAACG GCTGATGGTG AAATTCCGTG GGGAAGAAGG TTTGGATTAC 
GGTGGTGTGG CCAGGGAGTG GCTTTACTTG CTGTGCCATG AAATGCTGAA TCCTTATTAC 
GGGCTCTTCC AGTATTCTAC GGACAATATT TACATGTTGC AAATAAATCC GGATTCTTCA 
ATCAACCCCG ACCACTTGTC TTATTTCCAC TTTGTGGGGC GGATCATGGG GCTGGCTGTG 
TTCCATGGAC ACTACATCAA CGGGGGCTTC ACAGTGCCCT TCTACAAGCA GCTGCTGGGG 
AAGCCCATCC AGCTCTCAGA TCTGGAATCT GTGGACCCAG AGCTGCATAA GAGCTTGGTG 
TGGATCCTAG AGAACGACAT CACGCCTGTA CTGGACCACA CCTTCTGCGT GGAACACAAC 
GCCTTCGGGC GGATCCTGCA GCATGAACTG AAACCCAATG GCAGAAATGT GCCAGTCACA 
GAGGAGAATA AGAAAGAATA CGTCCGGTTG TATGTAAACT GGAGGTTTAT GAGAGGAATC 
GAAGCCCAGT TCTTAGCTCT GCAGAAGGGG TTCAATGAGC TCATCCCTCA ACATCTGCTG 
AAGCCTTTTG ACCAGAAGGA ACTGGAGCTG ATCATAGGCG GCCTGGATAA AATAGACTTG 
AACGACTGGA AGTCGAACAC GCGGCTGAAG CACTGTGTGG CCGACAGCAA CATCGTGCGG 
TGGTTCTGGC AAGCGGTGGA GACGTTCGAT GAAGAAAGGA GGGCCAGGCT CCTGCAGTTT 
GTGACTGGGT CCACGCGAGT CCCGCTCCAA GGCTTCAAGG CTTTGCAAGG TTCTACAGGC 
GCGGCAGGGC CCCGGCTGTT CACCATCCAC CTGATAGACG CGAACACAGA CAACCTTCCG 
AAGGCCCATA CCTGCTTTAA CCGGATCGAC ATTCCACCAT ATGAGTCCTA TGAGAAGCTC 
TACGAGAAGC TGCTGACAGC CGTGGAGGAG ACCTGCGGGT TTGCTGTGGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Upregulation of microRNA‑194‑5p inhibits hypopharyngeal carcinoma cell proliferation, migration and invasion by targeting SMURF1 via the mTOR signaling pathway.
International journal of oncology54(4)1245-1255(2019 Apr)
Xu S,Hui L,Yang N,Wang Y,Zhao N,Jiang XJ

Hippo kinase NDR2 inhibits IL-17 signaling by promoting Smurf1-mediated MEKK2 ubiquitination and degradation.
Molecular immunology105131-136(2019 01)
Ma X,Wang D,Li N,Gao P,Zhang M,Zhang Y

SMAD specific E3 ubiquitin protein ligase 1 promotes ovarian cancer cell migration and invasion via the activation of the RhoA/ROCK signaling pathway.
Oncology reports41(1)668-676(2019 Jan)
Wang W,Du H,Liu H,Hu F,Liu G

IRAK2 counterbalances oncogenic Smurf1 in colon cancer cells by dictating ER stress.
Cellular signalling4869-80(2018 Aug)
Liu J,Chen Y,Huang Q,Liu W,Ji X,Hu F,Zhu Y,Zhang L,Dong G

The E3 ubiquitin ligase SMURF1 regulates cell-fate specification and outflow tract septation during mammalian heart development.
Scientific reports8(1)9542(2018 06)
Koefoed K,Skat-Rørdam J,Andersen P,Warzecha CB,Pye M,Andersen TA,Ajbro KD,Bendsen E,Narimatsu M,Vilhardt F,Pedersen LB,Wrana JL,Anderson RH,Møllgård K,Christensen ST,Larsen LA

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

SMURF1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM