TYW1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TYW1
Entrez Gene ID	55253
Full Name	tRNA-yW synthesizing protein 1 homolog
Synonyms	RSAFD1,TYW1A,YPL207W
General protein information	Preferred Names tRNA-yW synthesizing protein 1 homolog Names S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase radical S-adenosyl methionine and flavodoxin domain-containing protein 1 radical S-adenosyl methionine and flavodoxin domains 1 tRNA wybutosine-synthesizing protein 1 homolog tRNA-yW synthesizing protein 1 homolog A
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q11.21
Summary	Wybutosine (yW) is a hypermodified guanosine found in phenylalanine tRNA adjacent to the anticodon that stabilizes codon-anticodon interactions in the ribosome. In yeast, the homolog of this gene is essential for the synthesis of wybutosine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015].
Disorder MIM:	611243

mRNA and Protein(s)

mRNA	Protein	Name
XM_017012392.1	XP_016867881.1	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X2
XM_011516372.2	XP_011514674.1	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X1
NM_018264.4	NP_060734.2	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1
NM_018264.3	NP_060734.2	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
XM_011516372.3	XP_011514674.1	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 isoform X1
XM_017012392.2	XP_016867881.1	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	TYW1	NP_060734.2
Mus musculus (house mouse)	Tyw1	NP_001015876.1
Schizosaccharomyces pombe (fission yeast)	SPCC1020.08	NP_587951.1
Macaca mulatta (Rhesus monkey)	TYW1	XP_001091335.2
Canis lupus familiaris (dog)	TYW1	XP_005620987.1
Saccharomyces cerevisiae (baker's yeast)	TYW1	NP_015117.1
Oryza sativa (rice)	Os03g0775300	NP_001051429.1
Eremothecium gossypii	AGOS_AFL099W	NP_985451.1
Neurospora crassa	NCU06633	XP_961821.1
Gallus gallus (chicken)	TYW1	XP_003642438.1
Arabidopsis thaliana (thale cress)	AT1G75200	NP_177656.2
Xenopus tropicalis (tropical clawed frog)	tyw1	NP_001096267.1
Magnaporthe oryzae (rice blast fungus)	MGG_06096	XP_003711962.1
Pan troglodytes (chimpanzee)	TYW1	XP_003318538.1
Danio rerio (zebrafish)	tyw1	NP_001070624.1
Bos taurus (cattle)	TYW1	NP_001179682.1
Rattus norvegicus (Norway rat)	Tyw1	NP_001100607.1

Gene Ontology
Bibliography

Related articles in PubMed

Ribonucleome analysis identified enzyme genes responsible for wybutosine synthesis.
Noma A, Suzuki T
Nucleic acids symposium series (2004)(50)65-6(2006)

Discovery of a gene family critical to wyosine base formation in a subset of phenylalanine-specific transfer RNAs.
Waas WF, de Crécy-Lagard V, Schimmel P
The Journal of biological chemistry280(45)37616-22(2005 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TYW1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TYW1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22102	NM_018264.4 Latest version!	Homo sapiens tRNA-yW synthesizing protein 1 homolog (TYW1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu69231	XM_011516372.3 Latest version!	Homo sapiens tRNA-yW synthesizing protein 1 homolog (TYW1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu97376	XM_017012392.2 Latest version!	Homo sapiens tRNA-yW synthesizing protein 1 homolog (TYW1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu22102	NM_018264.3	Homo sapiens tRNA-yW synthesizing protein 1 homolog (TYW1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu69231	XM_011516372.2	Homo sapiens tRNA-yW synthesizing protein 1 homolog (TYW1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu97376	XM_017012392.1	Homo sapiens tRNA-yW synthesizing protein 1 homolog (TYW1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TYW1 ( NM_018264.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060734.2 Homo sapiens tRNA-yW synthesizing protein 1 homolog (TYW1), transcript variant 1, mRNA.

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CloneID	OHu22102
Clone ID Related Accession (Same CDS sequence)	NM_018264.4 , NM_018264.3
Accession Version	NM_018264.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1566316800000
Organism	Homo sapiens(human)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC079920.6, AC073089.5 and AC006480.3. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_018264.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC015591.2, SRR1660809.63784.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000359626.10/ ENSP00000352645.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGATCCTT CTGCGGATAC ATGGGACCTC TTCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATTTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCAAGA CGCAGGGCAA GAACTTACAG GAAAAATCTG TTCCAAAAGC AGCTCAGGAT 
TTGATGACAA ATGGTTATGT CTCCCTTCAA GAGAAAGACA TCTTTGTGTC TGGAGTGAAG 
ATTTTTTATG GTTCTCAGAC TGGAACAGCG AAGGGATTCG CAACAGTTCT TGCTGAAGCA 
GTTACATCCC TGGATCTGCC TGTGGCCATT ATTAATCTAA AAGAATATGA TCCAGATGAT 
CATCTGATAG AAGAGGTGAC TAGTAAAAAT GTCTGTGTCT TCCTGGTTGC GACATACACT 
GACGGCCTAC CAACTGAAAG TGCAGAGTGG TTCTGCAAAT GGTTAGAGGA AGCATCCATT 
GATTTTCGAT TTGGCAAAAC TTACCTGAAG GGTATGAGAT ATGCGGTATT TGGCCTGGGA 
AATTCTGCCT ATGCTAGCCA CTTCAACAAG GTTGGCAAAA ATGTTGACAA GTGGCTCTGG 
ATGCTTGGCG CGCATCGTGT GATGAGTCGA GGGGAGGGCG ACTGCGACGT GGTTAAAAGC 
AAGCACGGCA GCATTGAGGC CGACTTCAGA GCATGGAAGA CCAAGTTCAT CTCCCAGCTG 
CAGGCACTTC AGAAAGGGGA GAGAAAGAAG TCCTGTGGCG GCCACTGCAA GAAAGGCAAA 
TGTGAATCTC ACCAACATGG CTCAGAGGAG AGGGAGGAAG GATCTCATGA GCAGGATGAA 
TTGCATCATA GAGACACCGA GGAGGAAGAA CCCTTTGAGA GCTCCAGTGA AGAAGAGTTT 
GGTGGTGAGG ACCATCAGAG CCTAAATTCC ATTGTTGATG TTGAAGATTT GGGCAAAATT 
ATGGATCATG TGAAGAAAGA AAAGAGAGAA AAGGAACAGC AGGAAGAGAA GTCTGGTTTG 
TTCAGGAACA TGGGGAGGAA TGAAGATGGT GAAAGAAGAG CTATGATAAC TCCTGCTCTC 
CGAGAAGCCC TTACTAAACA AGGTTATCAG TTGATTGGGA GCCACTCGGG GGTGAAGCTT 
TGCAGGTGGA CAAAGTCCAT GCTCCGAGGG AGAGGAGGTT GTTACAAACA CACATTCTAT 
GGAATTGAGA GCCATCGCTG CATGGAAACC ACCCCGAGCT TGGCGTGTGC TAATAAATGT 
GTCTTCTGTT GGCGGCACCA CACCAACCCC GTGGGCACTG AGTGGCGGTG GAAGATGGAC 
CAGCCTGAAA TGATCTTGAA GGAAGCCATT GAAAACCATC AGAACATGAT TAAGCAGTTT 
AAAGGAGTAC CGGGCGTCAA AGCAGAACGC TTTGAAGAAG GAATGACGGT AAAGCACTGT 
GCATTGTCCC TCGTGGGAGA ACCAATAATG TACCCAGAGA TCAACAGGTT TTTGAAGCTA 
CTCCACCAGT GTAAAATTTC CAGCTTCCTG GTCACAAACG CACAATTTCC TGCGGAAATC 
AGGAACCTCG AGCCGGTTAC TCAGCTGTAT GTCAGTGTGG ATGCCAGTAC CAAAGACAGC 
CTGAAGAAAA TCGACCGCCC ACTCTTCAAG GATTTCTGGC AGAGATTCCT TGACAGTTTA 
AAAGCCTTGG CAGTCAAGCA ACAACGAACT GTCTACAGAC TGACGCTCGT GAAAGCATGG 
AACGTGGACG AGCTCCAGGC CTACGCGCAG CTCGTGTCCC TGGGGAATCC TGACTTCATC 
GAAGTGAAGG GCGTTACCTA CTGCGGAGAA AGTTCAGCAA GCAGTCTTAC CATGGCCCAC 
GTGCCCTGGC ATGAGGAAGT GGTACAGTTT GTCCACGAGT TGGTGGATCT GATCCCCGAA 
TATGAAATTG CATGTGAACA CGAACACTCT AATTGCCTCC TGATAGCACA CAGAAAGTTT 
AAAATTGGTG GTGAATGGTG GACATGGATC GATTATAACC GCTTCCAGGA GCTCATCCAG 
GAATATGAAG ATAGTGGTGG ATCAAAAACG TTCAGCGCAA AGGATTATAT GGCCAGAACT 
CCTCACTGGG CATTATTTGG TGCCAGTGAA AGAGGCTTTG ATCCCAAGGA CACAAGACAT 
CAGAGAAAGA ACAAATCAAA GGCTATTTCT GGATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060734.2
CDS	147..2345
Translation	MDPSADTWDLFSPLISLWINRFYIYLGFAVSISLWICVQIVIKTQGKNLQEKSVPKAAQDLMTNGYVSLQEKDIFVSGVKIFYGSQTGTAKGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRYAVFGLGNSAYASHFNKVGKNVDKWLWMLGAHRVMSRGEGDCDVVKSKHGSIEADFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHGSEEREEGSHEQDELHHRDTEEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGKIMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQGYQLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLKLLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSLKALAVKQQRTVYRLTLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCGESSASSLTMAHVPWHEEVVQFVHELVDLIPEYEIACEHEHSNCLLIAHRKFKIGGEWWTWIDYNRFQELIQEYEDSGGSKTFSAKDYMARTPHWALFGASERGFDPKDTRHQRKNKSKAISGC

Target ORF information:

RefSeq Version	NM_018264.4
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA-yW synthesizing protein 1 homolog (TYW1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018264.4

ORF Insert Sequence:

ATGGATCCTT CTGCGGATAC ATGGGACCTC TTCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATTTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCAAGA CGCAGGGCAA GAACTTACAG GAAAAATCTG TTCCAAAAGC AGCTCAGGAT 
TTGATGACAA ATGGTTATGT CTCCCTTCAA GAGAAAGACA TCTTTGTGTC TGGAGTGAAG 
ATTTTTTATG GTTCTCAGAC TGGAACAGCG AAGGGATTCG CAACAGTTCT TGCTGAAGCA 
GTTACATCCC TGGATCTGCC TGTGGCCATT ATTAATCTAA AAGAATATGA TCCAGATGAT 
CATCTGATAG AAGAGGTGAC TAGTAAAAAT GTCTGTGTCT TCCTGGTTGC GACATACACT 
GACGGCCTAC CAACTGAAAG TGCAGAGTGG TTCTGCAAAT GGTTAGAGGA AGCATCCATT 
GATTTTCGAT TTGGCAAAAC TTACCTGAAG GGTATGAGAT ATGCGGTATT TGGCCTGGGA 
AATTCTGCCT ATGCTAGCCA CTTCAACAAG GTTGGCAAAA ATGTTGACAA GTGGCTCTGG 
ATGCTTGGCG CGCATCGTGT GATGAGTCGA GGGGAGGGCG ACTGCGACGT GGTTAAAAGC 
AAGCACGGCA GCATTGAGGC CGACTTCAGA GCATGGAAGA CCAAGTTCAT CTCCCAGCTG 
CAGGCACTTC AGAAAGGGGA GAGAAAGAAG TCCTGTGGCG GCCACTGCAA GAAAGGCAAA 
TGTGAATCTC ACCAACATGG CTCAGAGGAG AGGGAGGAAG GATCTCATGA GCAGGATGAA 
TTGCATCATA GAGACACCGA GGAGGAAGAA CCCTTTGAGA GCTCCAGTGA AGAAGAGTTT 
GGTGGTGAGG ACCATCAGAG CCTAAATTCC ATTGTTGATG TTGAAGATTT GGGCAAAATT 
ATGGATCATG TGAAGAAAGA AAAGAGAGAA AAGGAACAGC AGGAAGAGAA GTCTGGTTTG 
TTCAGGAACA TGGGGAGGAA TGAAGATGGT GAAAGAAGAG CTATGATAAC TCCTGCTCTC 
CGAGAAGCCC TTACTAAACA AGGTTATCAG TTGATTGGGA GCCACTCGGG GGTGAAGCTT 
TGCAGGTGGA CAAAGTCCAT GCTCCGAGGG AGAGGAGGTT GTTACAAACA CACATTCTAT 
GGAATTGAGA GCCATCGCTG CATGGAAACC ACCCCGAGCT TGGCGTGTGC TAATAAATGT 
GTCTTCTGTT GGCGGCACCA CACCAACCCC GTGGGCACTG AGTGGCGGTG GAAGATGGAC 
CAGCCTGAAA TGATCTTGAA GGAAGCCATT GAAAACCATC AGAACATGAT TAAGCAGTTT 
AAAGGAGTAC CGGGCGTCAA AGCAGAACGC TTTGAAGAAG GAATGACGGT AAAGCACTGT 
GCATTGTCCC TCGTGGGAGA ACCAATAATG TACCCAGAGA TCAACAGGTT TTTGAAGCTA 
CTCCACCAGT GTAAAATTTC CAGCTTCCTG GTCACAAACG CACAATTTCC TGCGGAAATC 
AGGAACCTCG AGCCGGTTAC TCAGCTGTAT GTCAGTGTGG ATGCCAGTAC CAAAGACAGC 
CTGAAGAAAA TCGACCGCCC ACTCTTCAAG GATTTCTGGC AGAGATTCCT TGACAGTTTA 
AAAGCCTTGG CAGTCAAGCA ACAACGAACT GTCTACAGAC TGACGCTCGT GAAAGCATGG 
AACGTGGACG AGCTCCAGGC CTACGCGCAG CTCGTGTCCC TGGGGAATCC TGACTTCATC 
GAAGTGAAGG GCGTTACCTA CTGCGGAGAA AGTTCAGCAA GCAGTCTTAC CATGGCCCAC 
GTGCCCTGGC ATGAGGAAGT GGTACAGTTT GTCCACGAGT TGGTGGATCT GATCCCCGAA 
TATGAAATTG CATGTGAACA CGAACACTCT AATTGCCTCC TGATAGCACA CAGAAAGTTT 
AAAATTGGTG GTGAATGGTG GACATGGATC GATTATAACC GCTTCCAGGA GCTCATCCAG 
GAATATGAAG ATAGTGGTGG ATCAAAAACG TTCAGCGCAA AGGATTATAT GGCCAGAACT 
CCTCACTGGG CATTATTTGG TGCCAGTGAA AGAGGCTTTG ATCCCAAGGA CACAAGACAT 
CAGAGAAAGA ACAAATCAAA GGCTATTTCT GGATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TYW1 ( NM_018264.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060734.2 Homo sapiens tRNA-yW synthesizing protein 1 homolog (TYW1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22102
Clone ID Related Accession (Same CDS sequence)	NM_018264.4 , NM_018264.3
Accession Version	NM_018264.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Homo sapiens(human)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC079920.6, AC073089.5 and AC006480.3. This sequence is a reference standard in the RefSeqGene project. On Dec 22, 2012 this sequence version replaced NM_018264.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.279800.1, SRR1660809.63784.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATCCTT CTGCGGATAC ATGGGACCTC TTCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATTTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCAAGA CGCAGGGCAA GAACTTACAG GAAAAATCTG TTCCAAAAGC AGCTCAGGAT 
TTGATGACAA ATGGTTATGT CTCCCTTCAA GAGAAAGACA TCTTTGTGTC TGGAGTGAAG 
ATTTTTTATG GTTCTCAGAC TGGAACAGCG AAGGGATTCG CAACAGTTCT TGCTGAAGCA 
GTTACATCCC TGGATCTGCC TGTGGCCATT ATTAATCTAA AAGAATATGA TCCAGATGAT 
CATCTGATAG AAGAGGTGAC TAGTAAAAAT GTCTGTGTCT TCCTGGTTGC GACATACACT 
GACGGCCTAC CAACTGAAAG TGCAGAGTGG TTCTGCAAAT GGTTAGAGGA AGCATCCATT 
GATTTTCGAT TTGGCAAAAC TTACCTGAAG GGTATGAGAT ATGCGGTATT TGGCCTGGGA 
AATTCTGCCT ATGCTAGCCA CTTCAACAAG GTTGGCAAAA ATGTTGACAA GTGGCTCTGG 
ATGCTTGGCG CGCATCGTGT GATGAGTCGA GGGGAGGGCG ACTGCGACGT GGTTAAAAGC 
AAGCACGGCA GCATTGAGGC CGACTTCAGA GCATGGAAGA CCAAGTTCAT CTCCCAGCTG 
CAGGCACTTC AGAAAGGGGA GAGAAAGAAG TCCTGTGGCG GCCACTGCAA GAAAGGCAAA 
TGTGAATCTC ACCAACATGG CTCAGAGGAG AGGGAGGAAG GATCTCATGA GCAGGATGAA 
TTGCATCATA GAGACACCGA GGAGGAAGAA CCCTTTGAGA GCTCCAGTGA AGAAGAGTTT 
GGTGGTGAGG ACCATCAGAG CCTAAATTCC ATTGTTGATG TTGAAGATTT GGGCAAAATT 
ATGGATCATG TGAAGAAAGA AAAGAGAGAA AAGGAACAGC AGGAAGAGAA GTCTGGTTTG 
TTCAGGAACA TGGGGAGGAA TGAAGATGGT GAAAGAAGAG CTATGATAAC TCCTGCTCTC 
CGAGAAGCCC TTACTAAACA AGGTTATCAG TTGATTGGGA GCCACTCGGG GGTGAAGCTT 
TGCAGGTGGA CAAAGTCCAT GCTCCGAGGG AGAGGAGGTT GTTACAAACA CACATTCTAT 
GGAATTGAGA GCCATCGCTG CATGGAAACC ACCCCGAGCT TGGCGTGTGC TAATAAATGT 
GTCTTCTGTT GGCGGCACCA CACCAACCCC GTGGGCACTG AGTGGCGGTG GAAGATGGAC 
CAGCCTGAAA TGATCTTGAA GGAAGCCATT GAAAACCATC AGAACATGAT TAAGCAGTTT 
AAAGGAGTAC CGGGCGTCAA AGCAGAACGC TTTGAAGAAG GAATGACGGT AAAGCACTGT 
GCATTGTCCC TCGTGGGAGA ACCAATAATG TACCCAGAGA TCAACAGGTT TTTGAAGCTA 
CTCCACCAGT GTAAAATTTC CAGCTTCCTG GTCACAAACG CACAATTTCC TGCGGAAATC 
AGGAACCTCG AGCCGGTTAC TCAGCTGTAT GTCAGTGTGG ATGCCAGTAC CAAAGACAGC 
CTGAAGAAAA TCGACCGCCC ACTCTTCAAG GATTTCTGGC AGAGATTCCT TGACAGTTTA 
AAAGCCTTGG CAGTCAAGCA ACAACGAACT GTCTACAGAC TGACGCTCGT GAAAGCATGG 
AACGTGGACG AGCTCCAGGC CTACGCGCAG CTCGTGTCCC TGGGGAATCC TGACTTCATC 
GAAGTGAAGG GCGTTACCTA CTGCGGAGAA AGTTCAGCAA GCAGTCTTAC CATGGCCCAC 
GTGCCCTGGC ATGAGGAAGT GGTACAGTTT GTCCACGAGT TGGTGGATCT GATCCCCGAA 
TATGAAATTG CATGTGAACA CGAACACTCT AATTGCCTCC TGATAGCACA CAGAAAGTTT 
AAAATTGGTG GTGAATGGTG GACATGGATC GATTATAACC GCTTCCAGGA GCTCATCCAG 
GAATATGAAG ATAGTGGTGG ATCAAAAACG TTCAGCGCAA AGGATTATAT GGCCAGAACT 
CCTCACTGGG CATTATTTGG TGCCAGTGAA AGAGGCTTTG ATCCCAAGGA CACAAGACAT 
CAGAGAAAGA ACAAATCAAA GGCTATTTCT GGATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060734.2
CDS	175..2373
Translation	MDPSADTWDLFSPLISLWINRFYIYLGFAVSISLWICVQIVIKTQGKNLQEKSVPKAAQDLMTNGYVSLQEKDIFVSGVKIFYGSQTGTAKGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRYAVFGLGNSAYASHFNKVGKNVDKWLWMLGAHRVMSRGEGDCDVVKSKHGSIEADFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHGSEEREEGSHEQDELHHRDTEEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGKIMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQGYQLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLKLLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSLKALAVKQQRTVYRLTLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCGESSASSLTMAHVPWHEEVVQFVHELVDLIPEYEIACEHEHSNCLLIAHRKFKIGGEWWTWIDYNRFQELIQEYEDSGGSKTFSAKDYMARTPHWALFGASERGFDPKDTRHQRKNKSKAISGC

Target ORF information:

RefSeq Version	NM_018264.3
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA-yW synthesizing protein 1 homolog (TYW1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018264.3

ORF Insert Sequence:

ATGGATCCTT CTGCGGATAC ATGGGACCTC TTCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATTTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCAAGA CGCAGGGCAA GAACTTACAG GAAAAATCTG TTCCAAAAGC AGCTCAGGAT 
TTGATGACAA ATGGTTATGT CTCCCTTCAA GAGAAAGACA TCTTTGTGTC TGGAGTGAAG 
ATTTTTTATG GTTCTCAGAC TGGAACAGCG AAGGGATTCG CAACAGTTCT TGCTGAAGCA 
GTTACATCCC TGGATCTGCC TGTGGCCATT ATTAATCTAA AAGAATATGA TCCAGATGAT 
CATCTGATAG AAGAGGTGAC TAGTAAAAAT GTCTGTGTCT TCCTGGTTGC GACATACACT 
GACGGCCTAC CAACTGAAAG TGCAGAGTGG TTCTGCAAAT GGTTAGAGGA AGCATCCATT 
GATTTTCGAT TTGGCAAAAC TTACCTGAAG GGTATGAGAT ATGCGGTATT TGGCCTGGGA 
AATTCTGCCT ATGCTAGCCA CTTCAACAAG GTTGGCAAAA ATGTTGACAA GTGGCTCTGG 
ATGCTTGGCG CGCATCGTGT GATGAGTCGA GGGGAGGGCG ACTGCGACGT GGTTAAAAGC 
AAGCACGGCA GCATTGAGGC CGACTTCAGA GCATGGAAGA CCAAGTTCAT CTCCCAGCTG 
CAGGCACTTC AGAAAGGGGA GAGAAAGAAG TCCTGTGGCG GCCACTGCAA GAAAGGCAAA 
TGTGAATCTC ACCAACATGG CTCAGAGGAG AGGGAGGAAG GATCTCATGA GCAGGATGAA 
TTGCATCATA GAGACACCGA GGAGGAAGAA CCCTTTGAGA GCTCCAGTGA AGAAGAGTTT 
GGTGGTGAGG ACCATCAGAG CCTAAATTCC ATTGTTGATG TTGAAGATTT GGGCAAAATT 
ATGGATCATG TGAAGAAAGA AAAGAGAGAA AAGGAACAGC AGGAAGAGAA GTCTGGTTTG 
TTCAGGAACA TGGGGAGGAA TGAAGATGGT GAAAGAAGAG CTATGATAAC TCCTGCTCTC 
CGAGAAGCCC TTACTAAACA AGGTTATCAG TTGATTGGGA GCCACTCGGG GGTGAAGCTT 
TGCAGGTGGA CAAAGTCCAT GCTCCGAGGG AGAGGAGGTT GTTACAAACA CACATTCTAT 
GGAATTGAGA GCCATCGCTG CATGGAAACC ACCCCGAGCT TGGCGTGTGC TAATAAATGT 
GTCTTCTGTT GGCGGCACCA CACCAACCCC GTGGGCACTG AGTGGCGGTG GAAGATGGAC 
CAGCCTGAAA TGATCTTGAA GGAAGCCATT GAAAACCATC AGAACATGAT TAAGCAGTTT 
AAAGGAGTAC CGGGCGTCAA AGCAGAACGC TTTGAAGAAG GAATGACGGT AAAGCACTGT 
GCATTGTCCC TCGTGGGAGA ACCAATAATG TACCCAGAGA TCAACAGGTT TTTGAAGCTA 
CTCCACCAGT GTAAAATTTC CAGCTTCCTG GTCACAAACG CACAATTTCC TGCGGAAATC 
AGGAACCTCG AGCCGGTTAC TCAGCTGTAT GTCAGTGTGG ATGCCAGTAC CAAAGACAGC 
CTGAAGAAAA TCGACCGCCC ACTCTTCAAG GATTTCTGGC AGAGATTCCT TGACAGTTTA 
AAAGCCTTGG CAGTCAAGCA ACAACGAACT GTCTACAGAC TGACGCTCGT GAAAGCATGG 
AACGTGGACG AGCTCCAGGC CTACGCGCAG CTCGTGTCCC TGGGGAATCC TGACTTCATC 
GAAGTGAAGG GCGTTACCTA CTGCGGAGAA AGTTCAGCAA GCAGTCTTAC CATGGCCCAC 
GTGCCCTGGC ATGAGGAAGT GGTACAGTTT GTCCACGAGT TGGTGGATCT GATCCCCGAA 
TATGAAATTG CATGTGAACA CGAACACTCT AATTGCCTCC TGATAGCACA CAGAAAGTTT 
AAAATTGGTG GTGAATGGTG GACATGGATC GATTATAACC GCTTCCAGGA GCTCATCCAG 
GAATATGAAG ATAGTGGTGG ATCAAAAACG TTCAGCGCAA AGGATTATAT GGCCAGAACT 
CCTCACTGGG CATTATTTGG TGCCAGTGAA AGAGGCTTTG ATCCCAAGGA CACAAGACAT 
CAGAGAAAGA ACAAATCAAA GGCTATTTCT GGATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TYW1 ( XM_011516372.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514674.1 Homo sapiens tRNA-yW synthesizing protein 1 homolog (TYW1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu69231
Clone ID Related Accession (Same CDS sequence)	XM_011516372.2 , XM_011516372.3
Accession Version	XM_011516372.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011516372.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTT CTGCGGATAC ATGGGACCTC TTCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATTTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCAAGA CGCAGGGCAA GAACTTACAG GAAAAATCTG TTCCAAAAGC AGCTCAGGAT 
TTGATGACAA ATGGTTATGT CTCCCTTCAA GAGAAAGACA TCTTTGTGTC TGGAGTGAAG 
ATTTTTTATG GTTCTCAGAC TGGAACAGCG AAGGGATTCG CAACAGTTCT TGCTGAAGCA 
GTTACATCCC TGGATCTGCC TGTGGCCATT ATTAATCTAA AAGAATATGA TCCAGATGAT 
CATCTGATAG AAGAGGTGAC TAGTAAAAAT GTCTGTGTCT TCCTGGTTGC GACATACACT 
GACGGCCTAC CAACTGAAAG TGCAGAGTGG TTCTGCAAAT GGTTAGAGGA AGCATCCATT 
GATTTTCGAT TTGGCAAAAC TTACCTGAAG GGTATGAGAT ATGCGGTATT TGGCCTGGGA 
AATTCTGCCT ATGCTAGCCA CTTCAACAAG GTTGGCAAAA ATGTTGACAA GTGGCTCTGG 
ATGCTTGGCG CGCATCGTGT GATGAGTCGA GGGGAGGGCG ACTGCGACGT GGTTAAAAGC 
AAGCACGGCA GCATTGAGGC CGACTTCAGA GCATGGAAGA CCAAGTTCAT CTCCCAGCTG 
CAGGCACTTC AGAAAGGGGA GAGAAAGAAG TCCTGTGGCG GCCACTGCAA GAAAGGCAAA 
TGTGAATCTC ACCAACATGG CTCAGAGGAG AGGGAGGAAG GATCTCATGA GCAGGATGAA 
TTGCATCATA GAGACACCGA GGAGGAAGAA CCCTTTGAGA GCTCCAGTGA AGAAGAGTTT 
GGTGGTGAGG ACCATCAGAG CCTAAATTCC ATTGTTGATG TTGAAGATTT GGGCAAAATT 
ATGGATCATG TGAAGAAAGA AAAGAGAGAA AAGGAACAGC AGGAAGAGAA GTCTGGTTTG 
TTCAGGAACA TGGGGAGGAA TGAAGATGGT GAAAGAAGAG CTATGATAAC TCCTGCTCTC 
CGAGAAGCCC TTACTAAACA AGGTTATCAG TTGATTGGGA GCCACTCGGG GGTGAAGCTT 
TGCAGGTGGA CAAAGTCCAT GCTCCGAGGG AGAGGAGGTT GTTACAAACA CACATTCTAT 
GGAATTGAGA GCCATCGCTG CATGGAAACC ACCCCGAGCT TGGCGTGTGC TAATAAATGT 
GTCTTCTGTT GGCGGCACCA CACCAACCCC GTGGGCACTG AGTGGCGGTG GAAGATGGAC 
CAGCCTGAAA TGATCTTGAA GGAAGCCATT GAAAACCATC AGAACATGAT TAAGCAGTTT 
AAAGGAGTAC CGGGCGTCAA AGCAGAACGC TTTGAAGAAG GAATGACGGT AAAGCACTGT 
GCATTGTCCC TCGTGGGAGA ACCAATAATG TACCCAGAGA TCAACAGGTT TTTGAAGCTA 
CTCCACCAGT GTAAAATTTC CAGCTTCCTG GTCACAAACG CACAATTTCC TGCGGAAATC 
AGGAACCTCG AGCCGGTTAC TCAGCTGTAT GTCAGTGTGG ATGCCAGTAC CAAAGACAGC 
CTGAAGAAAA TCGACCGCCC ACTCTTCAAG GATTTCTGGC AGAGATTCCT TGACAGTTTA 
AAAGCCTTGG CAGTCAAGCA ACAACGAACT GTCTACAGAC TGACGCTCGT GAAAGCATGG 
AACGTGGACG AGCTCCAGGC CTACGCGCAG CTCGTGTCCC TGGGGAATCC TGACTTCATC 
GAAGTGAAGG GCGTTACCTA CTGCGGAGAA AGTTCAGCAA GCAGTCTTAC CATGGCCCAC 
GTGCCCTGGC ATGAGGAAGT GGTACAGTTT GTCCACGAGT TGGTGGATCT GATCCCCGAA 
TATGAAATTG CATGTGAACA CGAACACTCT AATTGCCTCC TGATAGCACA CAGAAAGGAT 
GAGTGCTTGA GAAATTTTGC AGACCTTGCA CTTGATGGAT CAGCTGGCAC CACCCAGCTC 
GATAAACTGG CTCATCTGAT CTTGTGGCCA CCACCCAGGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514674.1
CDS	163..2247
Translation	MDPSADTWDLFSPLISLWINRFYIYLGFAVSISLWICVQIVIKTQGKNLQEKSVPKAAQDLMTNGYVSLQEKDIFVSGVKIFYGSQTGTAKGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRYAVFGLGNSAYASHFNKVGKNVDKWLWMLGAHRVMSRGEGDCDVVKSKHGSIEADFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHGSEEREEGSHEQDELHHRDTEEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGKIMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQGYQLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLKLLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSLKALAVKQQRTVYRLTLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCGESSASSLTMAHVPWHEEVVQFVHELVDLIPEYEIACEHEHSNCLLIAHRKDECLRNFADLALDGSAGTTQLDKLAHLILWPPPRN

Target ORF information:

RefSeq Version	XM_011516372.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA-yW synthesizing protein 1 homolog (TYW1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011516372.2

ORF Insert Sequence:

ATGGATCCTT CTGCGGATAC ATGGGACCTC TTCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATTTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCAAGA CGCAGGGCAA GAACTTACAG GAAAAATCTG TTCCAAAAGC AGCTCAGGAT 
TTGATGACAA ATGGTTATGT CTCCCTTCAA GAGAAAGACA TCTTTGTGTC TGGAGTGAAG 
ATTTTTTATG GTTCTCAGAC TGGAACAGCG AAGGGATTCG CAACAGTTCT TGCTGAAGCA 
GTTACATCCC TGGATCTGCC TGTGGCCATT ATTAATCTAA AAGAATATGA TCCAGATGAT 
CATCTGATAG AAGAGGTGAC TAGTAAAAAT GTCTGTGTCT TCCTGGTTGC GACATACACT 
GACGGCCTAC CAACTGAAAG TGCAGAGTGG TTCTGCAAAT GGTTAGAGGA AGCATCCATT 
GATTTTCGAT TTGGCAAAAC TTACCTGAAG GGTATGAGAT ATGCGGTATT TGGCCTGGGA 
AATTCTGCCT ATGCTAGCCA CTTCAACAAG GTTGGCAAAA ATGTTGACAA GTGGCTCTGG 
ATGCTTGGCG CGCATCGTGT GATGAGTCGA GGGGAGGGCG ACTGCGACGT GGTTAAAAGC 
AAGCACGGCA GCATTGAGGC CGACTTCAGA GCATGGAAGA CCAAGTTCAT CTCCCAGCTG 
CAGGCACTTC AGAAAGGGGA GAGAAAGAAG TCCTGTGGCG GCCACTGCAA GAAAGGCAAA 
TGTGAATCTC ACCAACATGG CTCAGAGGAG AGGGAGGAAG GATCTCATGA GCAGGATGAA 
TTGCATCATA GAGACACCGA GGAGGAAGAA CCCTTTGAGA GCTCCAGTGA AGAAGAGTTT 
GGTGGTGAGG ACCATCAGAG CCTAAATTCC ATTGTTGATG TTGAAGATTT GGGCAAAATT 
ATGGATCATG TGAAGAAAGA AAAGAGAGAA AAGGAACAGC AGGAAGAGAA GTCTGGTTTG 
TTCAGGAACA TGGGGAGGAA TGAAGATGGT GAAAGAAGAG CTATGATAAC TCCTGCTCTC 
CGAGAAGCCC TTACTAAACA AGGTTATCAG TTGATTGGGA GCCACTCGGG GGTGAAGCTT 
TGCAGGTGGA CAAAGTCCAT GCTCCGAGGG AGAGGAGGTT GTTACAAACA CACATTCTAT 
GGAATTGAGA GCCATCGCTG CATGGAAACC ACCCCGAGCT TGGCGTGTGC TAATAAATGT 
GTCTTCTGTT GGCGGCACCA CACCAACCCC GTGGGCACTG AGTGGCGGTG GAAGATGGAC 
CAGCCTGAAA TGATCTTGAA GGAAGCCATT GAAAACCATC AGAACATGAT TAAGCAGTTT 
AAAGGAGTAC CGGGCGTCAA AGCAGAACGC TTTGAAGAAG GAATGACGGT AAAGCACTGT 
GCATTGTCCC TCGTGGGAGA ACCAATAATG TACCCAGAGA TCAACAGGTT TTTGAAGCTA 
CTCCACCAGT GTAAAATTTC CAGCTTCCTG GTCACAAACG CACAATTTCC TGCGGAAATC 
AGGAACCTCG AGCCGGTTAC TCAGCTGTAT GTCAGTGTGG ATGCCAGTAC CAAAGACAGC 
CTGAAGAAAA TCGACCGCCC ACTCTTCAAG GATTTCTGGC AGAGATTCCT TGACAGTTTA 
AAAGCCTTGG CAGTCAAGCA ACAACGAACT GTCTACAGAC TGACGCTCGT GAAAGCATGG 
AACGTGGACG AGCTCCAGGC CTACGCGCAG CTCGTGTCCC TGGGGAATCC TGACTTCATC 
GAAGTGAAGG GCGTTACCTA CTGCGGAGAA AGTTCAGCAA GCAGTCTTAC CATGGCCCAC 
GTGCCCTGGC ATGAGGAAGT GGTACAGTTT GTCCACGAGT TGGTGGATCT GATCCCCGAA 
TATGAAATTG CATGTGAACA CGAACACTCT AATTGCCTCC TGATAGCACA CAGAAAGGAT 
GAGTGCTTGA GAAATTTTGC AGACCTTGCA CTTGATGGAT CAGCTGGCAC CACCCAGCTC 
GATAAACTGG CTCATCTGAT CTTGTGGCCA CCACCCAGGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TYW1 ( XM_011516372.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514674.1 Homo sapiens tRNA-yW synthesizing protein 1 homolog (TYW1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu69231
Clone ID Related Accession (Same CDS sequence)	XM_011516372.2 , XM_011516372.3
Accession Version	XM_011516372.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011516372.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTT CTGCGGATAC ATGGGACCTC TTCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATTTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCAAGA CGCAGGGCAA GAACTTACAG GAAAAATCTG TTCCAAAAGC AGCTCAGGAT 
TTGATGACAA ATGGTTATGT CTCCCTTCAA GAGAAAGACA TCTTTGTGTC TGGAGTGAAG 
ATTTTTTATG GTTCTCAGAC TGGAACAGCG AAGGGATTCG CAACAGTTCT TGCTGAAGCA 
GTTACATCCC TGGATCTGCC TGTGGCCATT ATTAATCTAA AAGAATATGA TCCAGATGAT 
CATCTGATAG AAGAGGTGAC TAGTAAAAAT GTCTGTGTCT TCCTGGTTGC GACATACACT 
GACGGCCTAC CAACTGAAAG TGCAGAGTGG TTCTGCAAAT GGTTAGAGGA AGCATCCATT 
GATTTTCGAT TTGGCAAAAC TTACCTGAAG GGTATGAGAT ATGCGGTATT TGGCCTGGGA 
AATTCTGCCT ATGCTAGCCA CTTCAACAAG GTTGGCAAAA ATGTTGACAA GTGGCTCTGG 
ATGCTTGGCG CGCATCGTGT GATGAGTCGA GGGGAGGGCG ACTGCGACGT GGTTAAAAGC 
AAGCACGGCA GCATTGAGGC CGACTTCAGA GCATGGAAGA CCAAGTTCAT CTCCCAGCTG 
CAGGCACTTC AGAAAGGGGA GAGAAAGAAG TCCTGTGGCG GCCACTGCAA GAAAGGCAAA 
TGTGAATCTC ACCAACATGG CTCAGAGGAG AGGGAGGAAG GATCTCATGA GCAGGATGAA 
TTGCATCATA GAGACACCGA GGAGGAAGAA CCCTTTGAGA GCTCCAGTGA AGAAGAGTTT 
GGTGGTGAGG ACCATCAGAG CCTAAATTCC ATTGTTGATG TTGAAGATTT GGGCAAAATT 
ATGGATCATG TGAAGAAAGA AAAGAGAGAA AAGGAACAGC AGGAAGAGAA GTCTGGTTTG 
TTCAGGAACA TGGGGAGGAA TGAAGATGGT GAAAGAAGAG CTATGATAAC TCCTGCTCTC 
CGAGAAGCCC TTACTAAACA AGGTTATCAG TTGATTGGGA GCCACTCGGG GGTGAAGCTT 
TGCAGGTGGA CAAAGTCCAT GCTCCGAGGG AGAGGAGGTT GTTACAAACA CACATTCTAT 
GGAATTGAGA GCCATCGCTG CATGGAAACC ACCCCGAGCT TGGCGTGTGC TAATAAATGT 
GTCTTCTGTT GGCGGCACCA CACCAACCCC GTGGGCACTG AGTGGCGGTG GAAGATGGAC 
CAGCCTGAAA TGATCTTGAA GGAAGCCATT GAAAACCATC AGAACATGAT TAAGCAGTTT 
AAAGGAGTAC CGGGCGTCAA AGCAGAACGC TTTGAAGAAG GAATGACGGT AAAGCACTGT 
GCATTGTCCC TCGTGGGAGA ACCAATAATG TACCCAGAGA TCAACAGGTT TTTGAAGCTA 
CTCCACCAGT GTAAAATTTC CAGCTTCCTG GTCACAAACG CACAATTTCC TGCGGAAATC 
AGGAACCTCG AGCCGGTTAC TCAGCTGTAT GTCAGTGTGG ATGCCAGTAC CAAAGACAGC 
CTGAAGAAAA TCGACCGCCC ACTCTTCAAG GATTTCTGGC AGAGATTCCT TGACAGTTTA 
AAAGCCTTGG CAGTCAAGCA ACAACGAACT GTCTACAGAC TGACGCTCGT GAAAGCATGG 
AACGTGGACG AGCTCCAGGC CTACGCGCAG CTCGTGTCCC TGGGGAATCC TGACTTCATC 
GAAGTGAAGG GCGTTACCTA CTGCGGAGAA AGTTCAGCAA GCAGTCTTAC CATGGCCCAC 
GTGCCCTGGC ATGAGGAAGT GGTACAGTTT GTCCACGAGT TGGTGGATCT GATCCCCGAA 
TATGAAATTG CATGTGAACA CGAACACTCT AATTGCCTCC TGATAGCACA CAGAAAGGAT 
GAGTGCTTGA GAAATTTTGC AGACCTTGCA CTTGATGGAT CAGCTGGCAC CACCCAGCTC 
GATAAACTGG CTCATCTGAT CTTGTGGCCA CCACCCAGGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514674.1
CDS	150..2234
Translation	MDPSADTWDLFSPLISLWINRFYIYLGFAVSISLWICVQIVIKTQGKNLQEKSVPKAAQDLMTNGYVSLQEKDIFVSGVKIFYGSQTGTAKGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRYAVFGLGNSAYASHFNKVGKNVDKWLWMLGAHRVMSRGEGDCDVVKSKHGSIEADFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHGSEEREEGSHEQDELHHRDTEEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGKIMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQGYQLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLKLLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSLKALAVKQQRTVYRLTLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCGESSASSLTMAHVPWHEEVVQFVHELVDLIPEYEIACEHEHSNCLLIAHRKDECLRNFADLALDGSAGTTQLDKLAHLILWPPPRN

Target ORF information:

RefSeq Version	XM_011516372.3
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA-yW synthesizing protein 1 homolog (TYW1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011516372.3

ORF Insert Sequence:

ATGGATCCTT CTGCGGATAC ATGGGACCTC TTCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATTTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCAAGA CGCAGGGCAA GAACTTACAG GAAAAATCTG TTCCAAAAGC AGCTCAGGAT 
TTGATGACAA ATGGTTATGT CTCCCTTCAA GAGAAAGACA TCTTTGTGTC TGGAGTGAAG 
ATTTTTTATG GTTCTCAGAC TGGAACAGCG AAGGGATTCG CAACAGTTCT TGCTGAAGCA 
GTTACATCCC TGGATCTGCC TGTGGCCATT ATTAATCTAA AAGAATATGA TCCAGATGAT 
CATCTGATAG AAGAGGTGAC TAGTAAAAAT GTCTGTGTCT TCCTGGTTGC GACATACACT 
GACGGCCTAC CAACTGAAAG TGCAGAGTGG TTCTGCAAAT GGTTAGAGGA AGCATCCATT 
GATTTTCGAT TTGGCAAAAC TTACCTGAAG GGTATGAGAT ATGCGGTATT TGGCCTGGGA 
AATTCTGCCT ATGCTAGCCA CTTCAACAAG GTTGGCAAAA ATGTTGACAA GTGGCTCTGG 
ATGCTTGGCG CGCATCGTGT GATGAGTCGA GGGGAGGGCG ACTGCGACGT GGTTAAAAGC 
AAGCACGGCA GCATTGAGGC CGACTTCAGA GCATGGAAGA CCAAGTTCAT CTCCCAGCTG 
CAGGCACTTC AGAAAGGGGA GAGAAAGAAG TCCTGTGGCG GCCACTGCAA GAAAGGCAAA 
TGTGAATCTC ACCAACATGG CTCAGAGGAG AGGGAGGAAG GATCTCATGA GCAGGATGAA 
TTGCATCATA GAGACACCGA GGAGGAAGAA CCCTTTGAGA GCTCCAGTGA AGAAGAGTTT 
GGTGGTGAGG ACCATCAGAG CCTAAATTCC ATTGTTGATG TTGAAGATTT GGGCAAAATT 
ATGGATCATG TGAAGAAAGA AAAGAGAGAA AAGGAACAGC AGGAAGAGAA GTCTGGTTTG 
TTCAGGAACA TGGGGAGGAA TGAAGATGGT GAAAGAAGAG CTATGATAAC TCCTGCTCTC 
CGAGAAGCCC TTACTAAACA AGGTTATCAG TTGATTGGGA GCCACTCGGG GGTGAAGCTT 
TGCAGGTGGA CAAAGTCCAT GCTCCGAGGG AGAGGAGGTT GTTACAAACA CACATTCTAT 
GGAATTGAGA GCCATCGCTG CATGGAAACC ACCCCGAGCT TGGCGTGTGC TAATAAATGT 
GTCTTCTGTT GGCGGCACCA CACCAACCCC GTGGGCACTG AGTGGCGGTG GAAGATGGAC 
CAGCCTGAAA TGATCTTGAA GGAAGCCATT GAAAACCATC AGAACATGAT TAAGCAGTTT 
AAAGGAGTAC CGGGCGTCAA AGCAGAACGC TTTGAAGAAG GAATGACGGT AAAGCACTGT 
GCATTGTCCC TCGTGGGAGA ACCAATAATG TACCCAGAGA TCAACAGGTT TTTGAAGCTA 
CTCCACCAGT GTAAAATTTC CAGCTTCCTG GTCACAAACG CACAATTTCC TGCGGAAATC 
AGGAACCTCG AGCCGGTTAC TCAGCTGTAT GTCAGTGTGG ATGCCAGTAC CAAAGACAGC 
CTGAAGAAAA TCGACCGCCC ACTCTTCAAG GATTTCTGGC AGAGATTCCT TGACAGTTTA 
AAAGCCTTGG CAGTCAAGCA ACAACGAACT GTCTACAGAC TGACGCTCGT GAAAGCATGG 
AACGTGGACG AGCTCCAGGC CTACGCGCAG CTCGTGTCCC TGGGGAATCC TGACTTCATC 
GAAGTGAAGG GCGTTACCTA CTGCGGAGAA AGTTCAGCAA GCAGTCTTAC CATGGCCCAC 
GTGCCCTGGC ATGAGGAAGT GGTACAGTTT GTCCACGAGT TGGTGGATCT GATCCCCGAA 
TATGAAATTG CATGTGAACA CGAACACTCT AATTGCCTCC TGATAGCACA CAGAAAGGAT 
GAGTGCTTGA GAAATTTTGC AGACCTTGCA CTTGATGGAT CAGCTGGCAC CACCCAGCTC 
GATAAACTGG CTCATCTGAT CTTGTGGCCA CCACCCAGGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TYW1 ( XM_017012392.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867881.1 Homo sapiens tRNA-yW synthesizing protein 1 homolog (TYW1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu97376
Clone ID Related Accession (Same CDS sequence)	XM_017012392.1 , XM_017012392.2
Accession Version	XM_017012392.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTT CTGCGGATAC ATGGGACCTC TTCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATTTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCAAGA CGCAGGGCAA GAACTTACAG GAAAAATCTG TTCCAAAAGC AGCTCAGGAT 
TTGATGACAA ATGGTTATGT CTCCCTTCAA GAGAAAGACA TCTTTGTGTC TGGAGTGAAG 
ATTTTTTATG GTTCTCAGAC TGGAACAGCG AAGGGATTCG CAACAGTTCT TGCTGAAGCA 
GTTACATCCC TGGATCTGCC TGTGGCCATT ATTAATCTAA AAGAATATGA TCCAGATGAT 
CATCTGATAG AAGAGGTGAC TAGTAAAAAT GTCTGTGTCT TCCTGGTTGC GACATACACT 
GACGGCCTAC CAACTGAAAG TGCAGAGTGG TTCTGCAAAT GGTTAGAGGA AGCATCCATT 
GATTTTCGAT TTGGCAAAAC TTACCTGAAG GGTATGAGAT ATGCGGTATT TGGCCTGGGA 
AATTCTGCCT ATGCTAGCCA CTTCAACAAG GTTGGCAAAA ATGTTGACAA GTGGCTCTGG 
ATGCTTGGCG CGCATCGTGT GATGAGTCGA GGGGAGGGCG ACTGCGACGT GGTTAAAAGC 
AAGCACGGCA GCATTGAGGC CGACTTCAGA GCATGGAAGA CCAAGTTCAT CTCCCAGCTG 
CAGGCACTTC AGAAAGGGGA GAGAAAGAAG TCCTGTGGCG GCCACTGCAA GAAAGGCAAA 
TGTGAATCTC ACCAACATGG CTCAGAGGAG AGGGAGGAAG GATCTCATGA GCAGGATGAA 
TTGCATCATA GAGACACCGA GGAGGAAGAA CCCTTTGAGA GCTCCAGTGA AGAAGAGTTT 
GGTGGTGAGG ACCATCAGAG CCTAAATTCC ATTGTTGATG TTGAAGATTT GGGCAAAATT 
ATGGATCATG TGAAGAAAGA AAAGAGAGAA AAGGAACAGC AGGAAGAGAA GTCTGGTTTG 
TTCAGGAACA TGGGGAGGAA TGAAGATGGT GAAAGAAGAG CTATGATAAC TCCTGCTCTC 
CGAGAAGCCC TTACTAAACA AGGAGTACCG GGCGTCAAAG CAGAACGCTT TGAAGAAGGA 
ATGACGGTAA AGCACTGTGC ATTGTCCCTC GTGGGAGAAC CAATAATGTA CCCAGAGATC 
AACAGGTTTT TGAAGCTACT CCACCAGTGT AAAATTTCCA GCTTCCTGGT CACAAACGCA 
CAATTTCCTG CGGAAATCAG GAACCTCGAG CCGGTTACTC AGCTGTATGT CAGTGTGGAT 
GCCAGTACCA AAGACAGCCT GAAGAAAATC GACCGCCCAC TCTTCAAGGA TTTCTGGCAG 
AGATTCCTTG ACAGTTTAAA AGCCTTGGCA GTCAAGCAAC AACGAACTGT CTACAGACTG 
ACGCTCGTGA AAGCATGGAA CGTGGACGAG CTCCAGGCCT ACGCGCAGCT CGTGTCCCTG 
GGGAATCCTG ACTTCATCGA AGTGAAGGGC GTTACCTACT GCGGAGAAAG TTCAGCAAGC 
AGTCTTACCA TGGCCCACGT GCCCTGGCAT GAGGAAGTGG TACAGTTTGT CCACGAGTTG 
GTGGATCTGA TCCCCGAATA TGAAATTGCA TGTGAACACG AACACTCTAA TTGCCTCCTG 
ATAGCACACA GAAAGTTTAA AATTGGTGGT GAATGGTGGA CATGGATCGA TTATAACCGC 
TTCCAGGAGC TCATCCAGGA ATATGAAGAT AGTGGTGGAT CAAAAACGTT CAGCGCAAAG 
GATTATATGG CCAGAACTCC TCACTGGGCA TTATTTGGTG CCAGTGAAAG AGGCTTTGAT 
CCCAAGGACA CAAGACATCA GAGAAAGAAC AAATCAAAGG CTATTTCTGG ATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867881.1
CDS	163..2079
Translation	MDPSADTWDLFSPLISLWINRFYIYLGFAVSISLWICVQIVIKTQGKNLQEKSVPKAAQDLMTNGYVSLQEKDIFVSGVKIFYGSQTGTAKGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRYAVFGLGNSAYASHFNKVGKNVDKWLWMLGAHRVMSRGEGDCDVVKSKHGSIEADFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHGSEEREEGSHEQDELHHRDTEEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGKIMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLKLLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSLKALAVKQQRTVYRLTLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCGESSASSLTMAHVPWHEEVVQFVHELVDLIPEYEIACEHEHSNCLLIAHRKFKIGGEWWTWIDYNRFQELIQEYEDSGGSKTFSAKDYMARTPHWALFGASERGFDPKDTRHQRKNKSKAISGC

Target ORF information:

RefSeq Version	XM_017012392.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA-yW synthesizing protein 1 homolog (TYW1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012392.1

ORF Insert Sequence:

ATGGATCCTT CTGCGGATAC ATGGGACCTC TTCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATTTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCAAGA CGCAGGGCAA GAACTTACAG GAAAAATCTG TTCCAAAAGC AGCTCAGGAT 
TTGATGACAA ATGGTTATGT CTCCCTTCAA GAGAAAGACA TCTTTGTGTC TGGAGTGAAG 
ATTTTTTATG GTTCTCAGAC TGGAACAGCG AAGGGATTCG CAACAGTTCT TGCTGAAGCA 
GTTACATCCC TGGATCTGCC TGTGGCCATT ATTAATCTAA AAGAATATGA TCCAGATGAT 
CATCTGATAG AAGAGGTGAC TAGTAAAAAT GTCTGTGTCT TCCTGGTTGC GACATACACT 
GACGGCCTAC CAACTGAAAG TGCAGAGTGG TTCTGCAAAT GGTTAGAGGA AGCATCCATT 
GATTTTCGAT TTGGCAAAAC TTACCTGAAG GGTATGAGAT ATGCGGTATT TGGCCTGGGA 
AATTCTGCCT ATGCTAGCCA CTTCAACAAG GTTGGCAAAA ATGTTGACAA GTGGCTCTGG 
ATGCTTGGCG CGCATCGTGT GATGAGTCGA GGGGAGGGCG ACTGCGACGT GGTTAAAAGC 
AAGCACGGCA GCATTGAGGC CGACTTCAGA GCATGGAAGA CCAAGTTCAT CTCCCAGCTG 
CAGGCACTTC AGAAAGGGGA GAGAAAGAAG TCCTGTGGCG GCCACTGCAA GAAAGGCAAA 
TGTGAATCTC ACCAACATGG CTCAGAGGAG AGGGAGGAAG GATCTCATGA GCAGGATGAA 
TTGCATCATA GAGACACCGA GGAGGAAGAA CCCTTTGAGA GCTCCAGTGA AGAAGAGTTT 
GGTGGTGAGG ACCATCAGAG CCTAAATTCC ATTGTTGATG TTGAAGATTT GGGCAAAATT 
ATGGATCATG TGAAGAAAGA AAAGAGAGAA AAGGAACAGC AGGAAGAGAA GTCTGGTTTG 
TTCAGGAACA TGGGGAGGAA TGAAGATGGT GAAAGAAGAG CTATGATAAC TCCTGCTCTC 
CGAGAAGCCC TTACTAAACA AGGAGTACCG GGCGTCAAAG CAGAACGCTT TGAAGAAGGA 
ATGACGGTAA AGCACTGTGC ATTGTCCCTC GTGGGAGAAC CAATAATGTA CCCAGAGATC 
AACAGGTTTT TGAAGCTACT CCACCAGTGT AAAATTTCCA GCTTCCTGGT CACAAACGCA 
CAATTTCCTG CGGAAATCAG GAACCTCGAG CCGGTTACTC AGCTGTATGT CAGTGTGGAT 
GCCAGTACCA AAGACAGCCT GAAGAAAATC GACCGCCCAC TCTTCAAGGA TTTCTGGCAG 
AGATTCCTTG ACAGTTTAAA AGCCTTGGCA GTCAAGCAAC AACGAACTGT CTACAGACTG 
ACGCTCGTGA AAGCATGGAA CGTGGACGAG CTCCAGGCCT ACGCGCAGCT CGTGTCCCTG 
GGGAATCCTG ACTTCATCGA AGTGAAGGGC GTTACCTACT GCGGAGAAAG TTCAGCAAGC 
AGTCTTACCA TGGCCCACGT GCCCTGGCAT GAGGAAGTGG TACAGTTTGT CCACGAGTTG 
GTGGATCTGA TCCCCGAATA TGAAATTGCA TGTGAACACG AACACTCTAA TTGCCTCCTG 
ATAGCACACA GAAAGTTTAA AATTGGTGGT GAATGGTGGA CATGGATCGA TTATAACCGC 
TTCCAGGAGC TCATCCAGGA ATATGAAGAT AGTGGTGGAT CAAAAACGTT CAGCGCAAAG 
GATTATATGG CCAGAACTCC TCACTGGGCA TTATTTGGTG CCAGTGAAAG AGGCTTTGAT 
CCCAAGGACA CAAGACATCA GAGAAAGAAC AAATCAAAGG CTATTTCTGG ATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TYW1 ( XM_017012392.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867881.1 Homo sapiens tRNA-yW synthesizing protein 1 homolog (TYW1), transcript variant X3, mRNA.

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CloneID	OHu97376
Clone ID Related Accession (Same CDS sequence)	XM_017012392.1 , XM_017012392.2
Accession Version	XM_017012392.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017012392.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCTT CTGCGGATAC ATGGGACCTC TTCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATTTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCAAGA CGCAGGGCAA GAACTTACAG GAAAAATCTG TTCCAAAAGC AGCTCAGGAT 
TTGATGACAA ATGGTTATGT CTCCCTTCAA GAGAAAGACA TCTTTGTGTC TGGAGTGAAG 
ATTTTTTATG GTTCTCAGAC TGGAACAGCG AAGGGATTCG CAACAGTTCT TGCTGAAGCA 
GTTACATCCC TGGATCTGCC TGTGGCCATT ATTAATCTAA AAGAATATGA TCCAGATGAT 
CATCTGATAG AAGAGGTGAC TAGTAAAAAT GTCTGTGTCT TCCTGGTTGC GACATACACT 
GACGGCCTAC CAACTGAAAG TGCAGAGTGG TTCTGCAAAT GGTTAGAGGA AGCATCCATT 
GATTTTCGAT TTGGCAAAAC TTACCTGAAG GGTATGAGAT ATGCGGTATT TGGCCTGGGA 
AATTCTGCCT ATGCTAGCCA CTTCAACAAG GTTGGCAAAA ATGTTGACAA GTGGCTCTGG 
ATGCTTGGCG CGCATCGTGT GATGAGTCGA GGGGAGGGCG ACTGCGACGT GGTTAAAAGC 
AAGCACGGCA GCATTGAGGC CGACTTCAGA GCATGGAAGA CCAAGTTCAT CTCCCAGCTG 
CAGGCACTTC AGAAAGGGGA GAGAAAGAAG TCCTGTGGCG GCCACTGCAA GAAAGGCAAA 
TGTGAATCTC ACCAACATGG CTCAGAGGAG AGGGAGGAAG GATCTCATGA GCAGGATGAA 
TTGCATCATA GAGACACCGA GGAGGAAGAA CCCTTTGAGA GCTCCAGTGA AGAAGAGTTT 
GGTGGTGAGG ACCATCAGAG CCTAAATTCC ATTGTTGATG TTGAAGATTT GGGCAAAATT 
ATGGATCATG TGAAGAAAGA AAAGAGAGAA AAGGAACAGC AGGAAGAGAA GTCTGGTTTG 
TTCAGGAACA TGGGGAGGAA TGAAGATGGT GAAAGAAGAG CTATGATAAC TCCTGCTCTC 
CGAGAAGCCC TTACTAAACA AGGAGTACCG GGCGTCAAAG CAGAACGCTT TGAAGAAGGA 
ATGACGGTAA AGCACTGTGC ATTGTCCCTC GTGGGAGAAC CAATAATGTA CCCAGAGATC 
AACAGGTTTT TGAAGCTACT CCACCAGTGT AAAATTTCCA GCTTCCTGGT CACAAACGCA 
CAATTTCCTG CGGAAATCAG GAACCTCGAG CCGGTTACTC AGCTGTATGT CAGTGTGGAT 
GCCAGTACCA AAGACAGCCT GAAGAAAATC GACCGCCCAC TCTTCAAGGA TTTCTGGCAG 
AGATTCCTTG ACAGTTTAAA AGCCTTGGCA GTCAAGCAAC AACGAACTGT CTACAGACTG 
ACGCTCGTGA AAGCATGGAA CGTGGACGAG CTCCAGGCCT ACGCGCAGCT CGTGTCCCTG 
GGGAATCCTG ACTTCATCGA AGTGAAGGGC GTTACCTACT GCGGAGAAAG TTCAGCAAGC 
AGTCTTACCA TGGCCCACGT GCCCTGGCAT GAGGAAGTGG TACAGTTTGT CCACGAGTTG 
GTGGATCTGA TCCCCGAATA TGAAATTGCA TGTGAACACG AACACTCTAA TTGCCTCCTG 
ATAGCACACA GAAAGTTTAA AATTGGTGGT GAATGGTGGA CATGGATCGA TTATAACCGC 
TTCCAGGAGC TCATCCAGGA ATATGAAGAT AGTGGTGGAT CAAAAACGTT CAGCGCAAAG 
GATTATATGG CCAGAACTCC TCACTGGGCA TTATTTGGTG CCAGTGAAAG AGGCTTTGAT 
CCCAAGGACA CAAGACATCA GAGAAAGAAC AAATCAAAGG CTATTTCTGG ATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867881.1
CDS	150..2066
Translation	MDPSADTWDLFSPLISLWINRFYIYLGFAVSISLWICVQIVIKTQGKNLQEKSVPKAAQDLMTNGYVSLQEKDIFVSGVKIFYGSQTGTAKGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRYAVFGLGNSAYASHFNKVGKNVDKWLWMLGAHRVMSRGEGDCDVVKSKHGSIEADFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHGSEEREEGSHEQDELHHRDTEEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGKIMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLKLLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSLKALAVKQQRTVYRLTLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCGESSASSLTMAHVPWHEEVVQFVHELVDLIPEYEIACEHEHSNCLLIAHRKFKIGGEWWTWIDYNRFQELIQEYEDSGGSKTFSAKDYMARTPHWALFGASERGFDPKDTRHQRKNKSKAISGC

Target ORF information:

RefSeq Version	XM_017012392.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tRNA-yW synthesizing protein 1 homolog (TYW1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012392.2

ORF Insert Sequence:

ATGGATCCTT CTGCGGATAC ATGGGACCTC TTCTCACCTT TAATATCATT ATGGATAAAC 
AGGTTTTACA TTTATTTGGG CTTTGCTGTT AGCATTAGCC TTTGGATTTG TGTCCAGATT 
GTCATCAAGA CGCAGGGCAA GAACTTACAG GAAAAATCTG TTCCAAAAGC AGCTCAGGAT 
TTGATGACAA ATGGTTATGT CTCCCTTCAA GAGAAAGACA TCTTTGTGTC TGGAGTGAAG 
ATTTTTTATG GTTCTCAGAC TGGAACAGCG AAGGGATTCG CAACAGTTCT TGCTGAAGCA 
GTTACATCCC TGGATCTGCC TGTGGCCATT ATTAATCTAA AAGAATATGA TCCAGATGAT 
CATCTGATAG AAGAGGTGAC TAGTAAAAAT GTCTGTGTCT TCCTGGTTGC GACATACACT 
GACGGCCTAC CAACTGAAAG TGCAGAGTGG TTCTGCAAAT GGTTAGAGGA AGCATCCATT 
GATTTTCGAT TTGGCAAAAC TTACCTGAAG GGTATGAGAT ATGCGGTATT TGGCCTGGGA 
AATTCTGCCT ATGCTAGCCA CTTCAACAAG GTTGGCAAAA ATGTTGACAA GTGGCTCTGG 
ATGCTTGGCG CGCATCGTGT GATGAGTCGA GGGGAGGGCG ACTGCGACGT GGTTAAAAGC 
AAGCACGGCA GCATTGAGGC CGACTTCAGA GCATGGAAGA CCAAGTTCAT CTCCCAGCTG 
CAGGCACTTC AGAAAGGGGA GAGAAAGAAG TCCTGTGGCG GCCACTGCAA GAAAGGCAAA 
TGTGAATCTC ACCAACATGG CTCAGAGGAG AGGGAGGAAG GATCTCATGA GCAGGATGAA 
TTGCATCATA GAGACACCGA GGAGGAAGAA CCCTTTGAGA GCTCCAGTGA AGAAGAGTTT 
GGTGGTGAGG ACCATCAGAG CCTAAATTCC ATTGTTGATG TTGAAGATTT GGGCAAAATT 
ATGGATCATG TGAAGAAAGA AAAGAGAGAA AAGGAACAGC AGGAAGAGAA GTCTGGTTTG 
TTCAGGAACA TGGGGAGGAA TGAAGATGGT GAAAGAAGAG CTATGATAAC TCCTGCTCTC 
CGAGAAGCCC TTACTAAACA AGGAGTACCG GGCGTCAAAG CAGAACGCTT TGAAGAAGGA 
ATGACGGTAA AGCACTGTGC ATTGTCCCTC GTGGGAGAAC CAATAATGTA CCCAGAGATC 
AACAGGTTTT TGAAGCTACT CCACCAGTGT AAAATTTCCA GCTTCCTGGT CACAAACGCA 
CAATTTCCTG CGGAAATCAG GAACCTCGAG CCGGTTACTC AGCTGTATGT CAGTGTGGAT 
GCCAGTACCA AAGACAGCCT GAAGAAAATC GACCGCCCAC TCTTCAAGGA TTTCTGGCAG 
AGATTCCTTG ACAGTTTAAA AGCCTTGGCA GTCAAGCAAC AACGAACTGT CTACAGACTG 
ACGCTCGTGA AAGCATGGAA CGTGGACGAG CTCCAGGCCT ACGCGCAGCT CGTGTCCCTG 
GGGAATCCTG ACTTCATCGA AGTGAAGGGC GTTACCTACT GCGGAGAAAG TTCAGCAAGC 
AGTCTTACCA TGGCCCACGT GCCCTGGCAT GAGGAAGTGG TACAGTTTGT CCACGAGTTG 
GTGGATCTGA TCCCCGAATA TGAAATTGCA TGTGAACACG AACACTCTAA TTGCCTCCTG 
ATAGCACACA GAAAGTTTAA AATTGGTGGT GAATGGTGGA CATGGATCGA TTATAACCGC 
TTCCAGGAGC TCATCCAGGA ATATGAAGAT AGTGGTGGAT CAAAAACGTT CAGCGCAAAG 
GATTATATGG CCAGAACTCC TCACTGGGCA TTATTTGGTG CCAGTGAAAG AGGCTTTGAT 
CCCAAGGACA CAAGACATCA GAGAAAGAAC AAATCAAAGG CTATTTCTGG ATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ribonucleome analysis identified enzyme genes responsible for wybutosine synthesis.
Nucleic acids symposium series (2004)(50)65-6(2006)
Noma A,Suzuki T

Discovery of a gene family critical to wyosine base formation in a subset of phenylalanine-specific transfer RNAs.
The Journal of biological chemistry280(45)37616-22(2005 Nov)
Waas WF,de Crécy-Lagard V,Schimmel P

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TYW1 cDNA ORF clone, Homo sapiens(human)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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