ZWILCH cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ZWILCH
Entrez Gene ID	55055
Full Name	zwilch kinetochore protein
Synonyms	KNTC1AP,hZwilch
General protein information	Preferred Names zwilch kinetochore protein Names protein zwilch homolog Zwilch, kinetochore associated, homolog homolog of Drosophila Zwilch
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q22.31
Disorder MIM:	609984

mRNA and Protein(s)

mRNA	Protein	Name
NM_017975.4	NP_060445.3	protein zwilch homolog isoform 1
NM_001287823.2	NP_001274752.1	protein zwilch homolog isoform 2
NM_001287821.1	NP_001274750.1	protein zwilch homolog isoform 2
NM_017975.5	NP_060445.3	protein zwilch homolog isoform 1
NM_001287821.2	NP_001274750.1	protein zwilch homolog isoform 2
NM_001287823.1	NP_001274752.1	protein zwilch homolog isoform 2
NM_001287822.1	NP_001274751.1	protein zwilch homolog isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	ZWILCH	NP_060445.3
Bos taurus (cattle)	ZWILCH	NP_001095649.1
Danio rerio (zebrafish)	zwilch	NP_001186664.1
Mus musculus (house mouse)	Zwilch	NP_080783.3
Gallus gallus (chicken)	ZWILCH	XP_004943968.1
Xenopus tropicalis (tropical clawed frog)	zwilch	NP_001016216.1
Macaca mulatta (Rhesus monkey)	ZWILCH	XP_001110821.2
Pan troglodytes (chimpanzee)	ZWILCH	XP_510495.1
Canis lupus familiaris (dog)	ZWILCH	XP_535523.3
Rattus norvegicus (Norway rat)	Zwilch	XP_006243310.1

Gene Ontology
Bibliography

Related articles in PubMed

Investigation of genetic susceptibility factors for human longevity - a targeted nonsynonymous SNP study.
Flachsbart F, Franke A, Kleindorp R, Caliebe A, Blanché H, Schreiber S, Nebel A
Mutation research694(1-2)13-9(2010 Dec)

Structural analysis of the RZZ complex reveals common ancestry with multisubunit vesicle tethering machinery.
Civril F, Wehenkel A, Giorgi FM, Santaguida S, Di Fonzo A, Grigorean G, Ciccarelli FD, Musacchio A
Structure (London, England : 1993)18(5)616-26(2010 May)

Single nucleotide polymorphisms in chromosomal instability genes and risk and clinical outcome of breast cancer: a Swedish prospective case-control study.
Brendle A, Brandt A, Johansson R, Enquist K, Hallmans G, Hemminki K, Lenner P, Försti A
European journal of cancer (Oxford, England : 1990)45(3)435-42(2009 Feb)

ZW10 links mitotic checkpoint signaling to the structural kinetochore.
Kops GJ, Kim Y, Weaver BA, Mao Y, McLeod I, Yates JR 3rd, Tagaya M, Cleveland DW
The Journal of cell biology169(1)49-60(2005 Apr)

Zwilch, a new component of the ZW10/ROD complex required for kinetochore functions.
Williams BC, Li Z, Liu S, Williams EV, Leung G, Yen TJ, Goldberg ML
Molecular biology of the cell14(4)1379-91(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ZWILCH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZWILCH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu12408	NM_017975.5 Latest version!	Homo sapiens zwilch kinetochore protein (ZWILCH), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu10802	NM_001287823.2 Latest version!	Homo sapiens zwilch kinetochore protein (ZWILCH), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu10802	NM_001287822.1 Latest version!	Homo sapiens zwilch kinetochore protein (ZWILCH), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu10802	NM_001287821.2 Latest version!	Homo sapiens zwilch kinetochore protein (ZWILCH), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
View Old Accession Versions >>
OHu12408	NM_017975.4	Homo sapiens zwilch kinetochore protein (ZWILCH), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu10802	NM_001287821.1	Homo sapiens zwilch kinetochore protein (ZWILCH), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu10802	NM_001287823.1	Homo sapiens zwilch kinetochore protein (ZWILCH), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZWILCH ( NM_017975.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060445.3 Homo sapiens zwilch kinetochore protein (ZWILCH), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu12408
Clone ID Related Accession (Same CDS sequence)	NM_017975.4 , NM_017975.5
Accession Version	NM_017975.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Homo sapiens(human)
Product	protein zwilch homolog isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC116913.6, BC036900.1 and BQ775697.1. On Dec 20, 2013 this sequence version replaced NM_017975.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.124908.1, SRR1803611.268778.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGAGC GGCTGAACTG CGCAGCAGAG GACTTTTATT CTCGTCTCCT TCAGAAATTT 
AATGAAGAAA AGAAAGGAAT CCGTAAAGAC CCATTTCTCT ATGAGGCTGA TGTCCAAGTG 
CAGTTGATCA GCAAAGGCCA ACCAAACCCT TTGAAAAATA TTCTAAATGA AAATGACATA 
GTATTCATAG TGGAAAAAGT GCCTTTAGAA AAGGAAGAAA CAAGTCATAT TGAAGAACTT 
CAATCTGAAG AAACTGCCAT ATCTGATTTC TCTACTGGCG AAAATGTTGG ACCACTTGCT 
TTACCAGTTG GGAAGGCAAG GCAGTTAATT GGACTTTACA CCATGGCTCA CAATCCTAAT 
ATGACCCATT TGAAGATTAA TCTGCCAGTT ACTGCCCTTC CTCCCCTTTG GGTAAGATGT 
GACAGTTCAG ATCCTGAAGG TACTTGTTGG CTAGGAGCTG AGCTTATCAC AACAAACAAC 
AGCATTACAG GAATTGTCTT ATATGTGGTC AGTTGTAAAG CTGATAAAAA TTATTCTGTA 
AATCTTGAAA ACCTAAAAAA TTTACACAAG AAAAGACATC ACTTGTCTAC TGTAACATCC 
AAAGGCTTTG CCCAGTATGA GCTCTTTAAG TCCTCTGCCT TGGATGATAC AATCACAGCA 
TCACAAACTG CGATCGCTTT GGATATTTCC TGGAGTCCTG TGGATGAGAT TCTTCAAATC 
CCTCCACTCT CTTCAACTGC AACTCTGAAT ATTAAAGTGG AATCAGGAGA GCCCAGAGGT 
CCTTTGAATC ATCTCTACAG AGAACTGAAA TTTCTTCTTG TTTTGGCTGA TGGTTTGAGG 
ACTGGTGTCA CTGAATGGCT CGAGCCCCTG GAAGCAAAAT CTGCTGTTGA ACTTGTTCAG 
GAATTTCTGA ATGACTTAAA TAAGCTGGAT GGATTTGGTG ATTCTACAAA AAAAGACACT 
GAGGTTGAGA CCTTGAAGCA TGACACTGCT GCAGTCGATC GTTCCGTCAA GCGTCTTTTC 
AAAGTTCGGA GTGATCTTGA TTTTGCTGAG CAACTGTGGT GCAAAATGAG CAGTAGTGTG 
ATTTCATACC AAGACTTGGT GAAGTGTTTC ACATTGATCA TCCAGAGTCT ACAACGTGGT 
GATATACAGC CATGGCTCCA TAGTGGAAGT AACAGTTTAC TAAGTAAGCT CATTCATCAG 
TCTTATCATG GAACCATGGA CACAGTTTCT CTCAGTGGGA CTATTCCAGT TCAAATGCTT 
TTGGAAATTG GTTTGGACAA ACTAAAGAAA GATTATATCA GTTTTTTCAT AGGTCAGGAA 
CTTGCATCTT TGAATCATTT GGAATACTTC ATTGCTCCAT CAGTAGATAT ACAAGAACAG 
GTTTATCGTG TCCAAAAACT CCACCATATT CTAGAAATAT TAGTCAGTTG CATGCCTTTC 
ATTAAATCTC AACATGAACT CCTCTTTTCT TTAACACAGA TCTGCATAAA GTATTACAAA 
CAAAATCCTC TTGATGAGCA ACACATTTTT CAGCTGCCAG TCAGACCAAC TGCTGTAAAG 
AACTTATATC AAAGTGAGAA GCCACAGAAA TGGAGAGTGG AAATATATAG TGGTCAAAAG 
AAGATTAAGA CAGTTTGGCA ACTGAGTGAC AGCTCACCCA TAGACCATCT GAATTTTCAC 
AAACCTGATT TTTCGGAATT AACACTAAAC GGTAGCCTGG AAGAAAGGAT ATTCTTTACT 
AACATGGTTA CCTGCAGCCA GGTGCATTTC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060445.3
CDS	257..2032
Translation	MWERLNCAAEDFYSRLLQKFNEEKKGIRKDPFLYEADVQVQLISKGQPNPLKNILNENDIVFIVEKVPLEKEETSHIEELQSEETAISDFSTGENVGPLALPVGKARQLIGLYTMAHNPNMTHLKINLPVTALPPLWVRCDSSDPEGTCWLGAELITTNNSITGIVLYVVSCKADKNYSVNLENLKNLHKKRHHLSTVTSKGFAQYELFKSSALDDTITASQTAIALDISWSPVDEILQIPPLSSTATLNIKVESGEPRGPLNHLYRELKFLLVLADGLRTGVTEWLEPLEAKSAVELVQEFLNDLNKLDGFGDSTKKDTEVETLKHDTAAVDRSVKRLFKVRSDLDFAEQLWCKMSSSVISYQDLVKCFTLIIQSLQRGDIQPWLHSGSNSLLSKLIHQSYHGTMDTVSLSGTIPVQMLLEIGLDKLKKDYISFFIGQELASLNHLEYFIAPSVDIQEQVYRVQKLHHILEILVSCMPFIKSQHELLFSLTQICIKYYKQNPLDEQHIFQLPVRPTAVKNLYQSEKPQKWRVEIYSGQKKIKTVWQLSDSSPIDHLNFHKPDFSELTLNGSLEERIFFTNMVTCSQVHFK

Target ORF information:

RefSeq Version	NM_017975.4
Organism	Homo sapiens(human)
Definition	Homo sapiens zwilch kinetochore protein (ZWILCH), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017975.4

ORF Insert Sequence:

ATGTGGGAGC GGCTGAACTG CGCAGCAGAG GACTTTTATT CTCGTCTCCT TCAGAAATTT 
AATGAAGAAA AGAAAGGAAT CCGTAAAGAC CCATTTCTCT ATGAGGCTGA TGTCCAAGTG 
CAGTTGATCA GCAAAGGCCA ACCAAACCCT TTGAAAAATA TTCTAAATGA AAATGACATA 
GTATTCATAG TGGAAAAAGT GCCTTTAGAA AAGGAAGAAA CAAGTCATAT TGAAGAACTT 
CAATCTGAAG AAACTGCCAT ATCTGATTTC TCTACTGGCG AAAATGTTGG ACCACTTGCT 
TTACCAGTTG GGAAGGCAAG GCAGTTAATT GGACTTTACA CCATGGCTCA CAATCCTAAT 
ATGACCCATT TGAAGATTAA TCTGCCAGTT ACTGCCCTTC CTCCCCTTTG GGTAAGATGT 
GACAGTTCAG ATCCTGAAGG TACTTGTTGG CTAGGAGCTG AGCTTATCAC AACAAACAAC 
AGCATTACAG GAATTGTCTT ATATGTGGTC AGTTGTAAAG CTGATAAAAA TTATTCTGTA 
AATCTTGAAA ACCTAAAAAA TTTACACAAG AAAAGACATC ACTTGTCTAC TGTAACATCC 
AAAGGCTTTG CCCAGTATGA GCTCTTTAAG TCCTCTGCCT TGGATGATAC AATCACAGCA 
TCACAAACTG CGATCGCTTT GGATATTTCC TGGAGTCCTG TGGATGAGAT TCTTCAAATC 
CCTCCACTCT CTTCAACTGC AACTCTGAAT ATTAAAGTGG AATCAGGAGA GCCCAGAGGT 
CCTTTGAATC ATCTCTACAG AGAACTGAAA TTTCTTCTTG TTTTGGCTGA TGGTTTGAGG 
ACTGGTGTCA CTGAATGGCT CGAGCCCCTG GAAGCAAAAT CTGCTGTTGA ACTTGTTCAG 
GAATTTCTGA ATGACTTAAA TAAGCTGGAT GGATTTGGTG ATTCTACAAA AAAAGACACT 
GAGGTTGAGA CCTTGAAGCA TGACACTGCT GCAGTCGATC GTTCCGTCAA GCGTCTTTTC 
AAAGTTCGGA GTGATCTTGA TTTTGCTGAG CAACTGTGGT GCAAAATGAG CAGTAGTGTG 
ATTTCATACC AAGACTTGGT GAAGTGTTTC ACATTGATCA TCCAGAGTCT ACAACGTGGT 
GATATACAGC CATGGCTCCA TAGTGGAAGT AACAGTTTAC TAAGTAAGCT CATTCATCAG 
TCTTATCATG GAACCATGGA CACAGTTTCT CTCAGTGGGA CTATTCCAGT TCAAATGCTT 
TTGGAAATTG GTTTGGACAA ACTAAAGAAA GATTATATCA GTTTTTTCAT AGGTCAGGAA 
CTTGCATCTT TGAATCATTT GGAATACTTC ATTGCTCCAT CAGTAGATAT ACAAGAACAG 
GTTTATCGTG TCCAAAAACT CCACCATATT CTAGAAATAT TAGTCAGTTG CATGCCTTTC 
ATTAAATCTC AACATGAACT CCTCTTTTCT TTAACACAGA TCTGCATAAA GTATTACAAA 
CAAAATCCTC TTGATGAGCA ACACATTTTT CAGCTGCCAG TCAGACCAAC TGCTGTAAAG 
AACTTATATC AAAGTGAGAA GCCACAGAAA TGGAGAGTGG AAATATATAG TGGTCAAAAG 
AAGATTAAGA CAGTTTGGCA ACTGAGTGAC AGCTCACCCA TAGACCATCT GAATTTTCAC 
AAACCTGATT TTTCGGAATT AACACTAAAC GGTAGCCTGG AAGAAAGGAT ATTCTTTACT 
AACATGGTTA CCTGCAGCCA GGTGCATTTC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZWILCH ( NM_017975.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_060445.3 Homo sapiens zwilch kinetochore protein (ZWILCH), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu12408
Clone ID Related Accession (Same CDS sequence)	NM_017975.4 , NM_017975.5
Accession Version	NM_017975.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556640000000
Organism	Homo sapiens(human)
Product	protein zwilch homolog isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC116913.6, BC036900.1 and BQ775697.1. On Nov 23, 2018 this sequence version replaced NM_017975.4. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.124908.1, SRR1660805.71996.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000307897.10/ ENSP00000311429.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTGGGAGC GGCTGAACTG CGCAGCAGAG GACTTTTATT CTCGTCTCCT TCAGAAATTT 
AATGAAGAAA AGAAAGGAAT CCGTAAAGAC CCATTTCTCT ATGAGGCTGA TGTCCAAGTG 
CAGTTGATCA GCAAAGGCCA ACCAAACCCT TTGAAAAATA TTCTAAATGA AAATGACATA 
GTATTCATAG TGGAAAAAGT GCCTTTAGAA AAGGAAGAAA CAAGTCATAT TGAAGAACTT 
CAATCTGAAG AAACTGCCAT ATCTGATTTC TCTACTGGCG AAAATGTTGG ACCACTTGCT 
TTACCAGTTG GGAAGGCAAG GCAGTTAATT GGACTTTACA CCATGGCTCA CAATCCTAAT 
ATGACCCATT TGAAGATTAA TCTGCCAGTT ACTGCCCTTC CTCCCCTTTG GGTAAGATGT 
GACAGTTCAG ATCCTGAAGG TACTTGTTGG CTAGGAGCTG AGCTTATCAC AACAAACAAC 
AGCATTACAG GAATTGTCTT ATATGTGGTC AGTTGTAAAG CTGATAAAAA TTATTCTGTA 
AATCTTGAAA ACCTAAAAAA TTTACACAAG AAAAGACATC ACTTGTCTAC TGTAACATCC 
AAAGGCTTTG CCCAGTATGA GCTCTTTAAG TCCTCTGCCT TGGATGATAC AATCACAGCA 
TCACAAACTG CGATCGCTTT GGATATTTCC TGGAGTCCTG TGGATGAGAT TCTTCAAATC 
CCTCCACTCT CTTCAACTGC AACTCTGAAT ATTAAAGTGG AATCAGGAGA GCCCAGAGGT 
CCTTTGAATC ATCTCTACAG AGAACTGAAA TTTCTTCTTG TTTTGGCTGA TGGTTTGAGG 
ACTGGTGTCA CTGAATGGCT CGAGCCCCTG GAAGCAAAAT CTGCTGTTGA ACTTGTTCAG 
GAATTTCTGA ATGACTTAAA TAAGCTGGAT GGATTTGGTG ATTCTACAAA AAAAGACACT 
GAGGTTGAGA CCTTGAAGCA TGACACTGCT GCAGTCGATC GTTCCGTCAA GCGTCTTTTC 
AAAGTTCGGA GTGATCTTGA TTTTGCTGAG CAACTGTGGT GCAAAATGAG CAGTAGTGTG 
ATTTCATACC AAGACTTGGT GAAGTGTTTC ACATTGATCA TCCAGAGTCT ACAACGTGGT 
GATATACAGC CATGGCTCCA TAGTGGAAGT AACAGTTTAC TAAGTAAGCT CATTCATCAG 
TCTTATCATG GAACCATGGA CACAGTTTCT CTCAGTGGGA CTATTCCAGT TCAAATGCTT 
TTGGAAATTG GTTTGGACAA ACTAAAGAAA GATTATATCA GTTTTTTCAT AGGTCAGGAA 
CTTGCATCTT TGAATCATTT GGAATACTTC ATTGCTCCAT CAGTAGATAT ACAAGAACAG 
GTTTATCGTG TCCAAAAACT CCACCATATT CTAGAAATAT TAGTCAGTTG CATGCCTTTC 
ATTAAATCTC AACATGAACT CCTCTTTTCT TTAACACAGA TCTGCATAAA GTATTACAAA 
CAAAATCCTC TTGATGAGCA ACACATTTTT CAGCTGCCAG TCAGACCAAC TGCTGTAAAG 
AACTTATATC AAAGTGAGAA GCCACAGAAA TGGAGAGTGG AAATATATAG TGGTCAAAAG 
AAGATTAAGA CAGTTTGGCA ACTGAGTGAC AGCTCACCCA TAGACCATCT GAATTTTCAC 
AAACCTGATT TTTCGGAATT AACACTAAAC GGTAGCCTGG AAGAAAGGAT ATTCTTTACT 
AACATGGTTA CCTGCAGCCA GGTGCATTTC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060445.3
CDS	14..1789
Translation	MWERLNCAAEDFYSRLLQKFNEEKKGIRKDPFLYEADVQVQLISKGQPNPLKNILNENDIVFIVEKVPLEKEETSHIEELQSEETAISDFSTGENVGPLALPVGKARQLIGLYTMAHNPNMTHLKINLPVTALPPLWVRCDSSDPEGTCWLGAELITTNNSITGIVLYVVSCKADKNYSVNLENLKNLHKKRHHLSTVTSKGFAQYELFKSSALDDTITASQTAIALDISWSPVDEILQIPPLSSTATLNIKVESGEPRGPLNHLYRELKFLLVLADGLRTGVTEWLEPLEAKSAVELVQEFLNDLNKLDGFGDSTKKDTEVETLKHDTAAVDRSVKRLFKVRSDLDFAEQLWCKMSSSVISYQDLVKCFTLIIQSLQRGDIQPWLHSGSNSLLSKLIHQSYHGTMDTVSLSGTIPVQMLLEIGLDKLKKDYISFFIGQELASLNHLEYFIAPSVDIQEQVYRVQKLHHILEILVSCMPFIKSQHELLFSLTQICIKYYKQNPLDEQHIFQLPVRPTAVKNLYQSEKPQKWRVEIYSGQKKIKTVWQLSDSSPIDHLNFHKPDFSELTLNGSLEERIFFTNMVTCSQVHFK

Target ORF information:

RefSeq Version	NM_017975.5
Organism	Homo sapiens(human)
Definition	Homo sapiens zwilch kinetochore protein (ZWILCH), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017975.5

ORF Insert Sequence:

ATGTGGGAGC GGCTGAACTG CGCAGCAGAG GACTTTTATT CTCGTCTCCT TCAGAAATTT 
AATGAAGAAA AGAAAGGAAT CCGTAAAGAC CCATTTCTCT ATGAGGCTGA TGTCCAAGTG 
CAGTTGATCA GCAAAGGCCA ACCAAACCCT TTGAAAAATA TTCTAAATGA AAATGACATA 
GTATTCATAG TGGAAAAAGT GCCTTTAGAA AAGGAAGAAA CAAGTCATAT TGAAGAACTT 
CAATCTGAAG AAACTGCCAT ATCTGATTTC TCTACTGGCG AAAATGTTGG ACCACTTGCT 
TTACCAGTTG GGAAGGCAAG GCAGTTAATT GGACTTTACA CCATGGCTCA CAATCCTAAT 
ATGACCCATT TGAAGATTAA TCTGCCAGTT ACTGCCCTTC CTCCCCTTTG GGTAAGATGT 
GACAGTTCAG ATCCTGAAGG TACTTGTTGG CTAGGAGCTG AGCTTATCAC AACAAACAAC 
AGCATTACAG GAATTGTCTT ATATGTGGTC AGTTGTAAAG CTGATAAAAA TTATTCTGTA 
AATCTTGAAA ACCTAAAAAA TTTACACAAG AAAAGACATC ACTTGTCTAC TGTAACATCC 
AAAGGCTTTG CCCAGTATGA GCTCTTTAAG TCCTCTGCCT TGGATGATAC AATCACAGCA 
TCACAAACTG CGATCGCTTT GGATATTTCC TGGAGTCCTG TGGATGAGAT TCTTCAAATC 
CCTCCACTCT CTTCAACTGC AACTCTGAAT ATTAAAGTGG AATCAGGAGA GCCCAGAGGT 
CCTTTGAATC ATCTCTACAG AGAACTGAAA TTTCTTCTTG TTTTGGCTGA TGGTTTGAGG 
ACTGGTGTCA CTGAATGGCT CGAGCCCCTG GAAGCAAAAT CTGCTGTTGA ACTTGTTCAG 
GAATTTCTGA ATGACTTAAA TAAGCTGGAT GGATTTGGTG ATTCTACAAA AAAAGACACT 
GAGGTTGAGA CCTTGAAGCA TGACACTGCT GCAGTCGATC GTTCCGTCAA GCGTCTTTTC 
AAAGTTCGGA GTGATCTTGA TTTTGCTGAG CAACTGTGGT GCAAAATGAG CAGTAGTGTG 
ATTTCATACC AAGACTTGGT GAAGTGTTTC ACATTGATCA TCCAGAGTCT ACAACGTGGT 
GATATACAGC CATGGCTCCA TAGTGGAAGT AACAGTTTAC TAAGTAAGCT CATTCATCAG 
TCTTATCATG GAACCATGGA CACAGTTTCT CTCAGTGGGA CTATTCCAGT TCAAATGCTT 
TTGGAAATTG GTTTGGACAA ACTAAAGAAA GATTATATCA GTTTTTTCAT AGGTCAGGAA 
CTTGCATCTT TGAATCATTT GGAATACTTC ATTGCTCCAT CAGTAGATAT ACAAGAACAG 
GTTTATCGTG TCCAAAAACT CCACCATATT CTAGAAATAT TAGTCAGTTG CATGCCTTTC 
ATTAAATCTC AACATGAACT CCTCTTTTCT TTAACACAGA TCTGCATAAA GTATTACAAA 
CAAAATCCTC TTGATGAGCA ACACATTTTT CAGCTGCCAG TCAGACCAAC TGCTGTAAAG 
AACTTATATC AAAGTGAGAA GCCACAGAAA TGGAGAGTGG AAATATATAG TGGTCAAAAG 
AAGATTAAGA CAGTTTGGCA ACTGAGTGAC AGCTCACCCA TAGACCATCT GAATTTTCAC 
AAACCTGATT TTTCGGAATT AACACTAAAC GGTAGCCTGG AAGAAAGGAT ATTCTTTACT 
AACATGGTTA CCTGCAGCCA GGTGCATTTC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZWILCH ( NM_001287823.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274752.1 Homo sapiens zwilch kinetochore protein (ZWILCH), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu10802
Clone ID Related Accession (Same CDS sequence)	NM_001287823.2 , NM_001287821.1 , NM_001287821.2 , NM_001287823.1 , NM_001287822.1
Accession Version	NM_001287823.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1559232000000
Organism	Homo sapiens(human)
Product	protein zwilch homolog isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK291048.1, BC036900.1, AC116913.6 and BQ775697.1. On Jun 1, 2019 this sequence version replaced NM_001287823.1. Transcript Variant: This variant (4) lacks an internal exon, compared to variant 1. This difference results in predicted translation initiation at a downstream in-frame start site through ribosomal re-initiation or leaky scanning. The encoded isoform (2) is shorter than isoform 1. Variants 2, 3, and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.158729.1, AK291048.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCACA ATCCTAATAT GACCCATTTG AAGATTAATC TGCCAGTTAC TGCCCTTCCT 
CCCCTTTGGG TAAGATGTGA CAGTTCAGAT CCTGAAGGTA CTTGTTGGCT AGGAGCTGAG 
CTTATCACAA CAAACAACAG CATTACAGGA ATTGTCTTAT ATGTGGTCAG TTGTAAAGCT 
GATAAAAATT ATTCTGTAAA TCTTGAAAAC CTAAAAAATT TACACAAGAA AAGACATCAC 
TTGTCTACTG TAACATCCAA AGGCTTTGCC CAGTATGAGC TCTTTAAGTC CTCTGCCTTG 
GATGATACAA TCACAGCATC ACAAACTGCG ATCGCTTTGG ATATTTCCTG GAGTCCTGTG 
GATGAGATTC TTCAAATCCC TCCACTCTCT TCAACTGCAA CTCTGAATAT TAAAGTGGAA 
TCAGGAGAGC CCAGAGGTCC TTTGAATCAT CTCTACAGAG AACTGAAATT TCTTCTTGTT 
TTGGCTGATG GTTTGAGGAC TGGTGTCACT GAATGGCTCG AGCCCCTGGA AGCAAAATCT 
GCTGTTGAAC TTGTTCAGGA ATTTCTGAAT GACTTAAATA AGCTGGATGG ATTTGGTGAT 
TCTACAAAAA AAGACACTGA GGTTGAGACC TTGAAGCATG ACACTGCTGC AGTCGATCGT 
TCCGTCAAGC GTCTTTTCAA AGTTCGGAGT GATCTTGATT TTGCTGAGCA ACTGTGGTGC 
AAAATGAGCA GTAGTGTGAT TTCATACCAA GACTTGGTGA AGTGTTTCAC ATTGATCATC 
CAGAGTCTAC AACGTGGTGA TATACAGCCA TGGCTCCATA GTGGAAGTAA CAGTTTACTA 
AGTAAGCTCA TTCATCAGTC TTATCATGGA ACCATGGACA CAGTTTCTCT CAGTGGGACT 
ATTCCAGTTC AAATGCTTTT GGAAATTGGT TTGGACAAAC TAAAGAAAGA TTATATCAGT 
TTTTTCATAG GTCAGGAACT TGCATCTTTG AATCATTTGG AATACTTCAT TGCTCCATCA 
GTAGATATAC AAGAACAGGT TTATCGTGTC CAAAAACTCC ACCATATTCT AGAAATATTA 
GTCAGTTGCA TGCCTTTCAT TAAATCTCAA CATGAACTCC TCTTTTCTTT AACACAGATC 
TGCATAAAGT ATTACAAACA AAATCCTCTT GATGAGCAAC ACATTTTTCA GCTGCCAGTC 
AGACCAACTG CTGTAAAGAA CTTATATCAA AGTGAGAAGC CACAGAAATG GAGAGTGGAA 
ATATATAGTG GTCAAAAGAA GATTAAGACA GTTTGGCAAC TGAGTGACAG CTCACCCATA 
GACCATCTGA ATTTTCACAA ACCTGATTTT TCGGAATTAA CACTAAACGG TAGCCTGGAA 
GAAAGGATAT TCTTTACTAA CATGGTTACC TGCAGCCAGG TGCATTTCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274752.1
CDS	304..1737
Translation	MAHNPNMTHLKINLPVTALPPLWVRCDSSDPEGTCWLGAELITTNNSITGIVLYVVSCKADKNYSVNLENLKNLHKKRHHLSTVTSKGFAQYELFKSSALDDTITASQTAIALDISWSPVDEILQIPPLSSTATLNIKVESGEPRGPLNHLYRELKFLLVLADGLRTGVTEWLEPLEAKSAVELVQEFLNDLNKLDGFGDSTKKDTEVETLKHDTAAVDRSVKRLFKVRSDLDFAEQLWCKMSSSVISYQDLVKCFTLIIQSLQRGDIQPWLHSGSNSLLSKLIHQSYHGTMDTVSLSGTIPVQMLLEIGLDKLKKDYISFFIGQELASLNHLEYFIAPSVDIQEQVYRVQKLHHILEILVSCMPFIKSQHELLFSLTQICIKYYKQNPLDEQHIFQLPVRPTAVKNLYQSEKPQKWRVEIYSGQKKIKTVWQLSDSSPIDHLNFHKPDFSELTLNGSLEERIFFTNMVTCSQVHFK

Target ORF information:

RefSeq Version	NM_001287823.2
Organism	Homo sapiens(human)
Definition	Homo sapiens zwilch kinetochore protein (ZWILCH), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287823.2

ORF Insert Sequence:

ATGGCTCACA ATCCTAATAT GACCCATTTG AAGATTAATC TGCCAGTTAC TGCCCTTCCT 
CCCCTTTGGG TAAGATGTGA CAGTTCAGAT CCTGAAGGTA CTTGTTGGCT AGGAGCTGAG 
CTTATCACAA CAAACAACAG CATTACAGGA ATTGTCTTAT ATGTGGTCAG TTGTAAAGCT 
GATAAAAATT ATTCTGTAAA TCTTGAAAAC CTAAAAAATT TACACAAGAA AAGACATCAC 
TTGTCTACTG TAACATCCAA AGGCTTTGCC CAGTATGAGC TCTTTAAGTC CTCTGCCTTG 
GATGATACAA TCACAGCATC ACAAACTGCG ATCGCTTTGG ATATTTCCTG GAGTCCTGTG 
GATGAGATTC TTCAAATCCC TCCACTCTCT TCAACTGCAA CTCTGAATAT TAAAGTGGAA 
TCAGGAGAGC CCAGAGGTCC TTTGAATCAT CTCTACAGAG AACTGAAATT TCTTCTTGTT 
TTGGCTGATG GTTTGAGGAC TGGTGTCACT GAATGGCTCG AGCCCCTGGA AGCAAAATCT 
GCTGTTGAAC TTGTTCAGGA ATTTCTGAAT GACTTAAATA AGCTGGATGG ATTTGGTGAT 
TCTACAAAAA AAGACACTGA GGTTGAGACC TTGAAGCATG ACACTGCTGC AGTCGATCGT 
TCCGTCAAGC GTCTTTTCAA AGTTCGGAGT GATCTTGATT TTGCTGAGCA ACTGTGGTGC 
AAAATGAGCA GTAGTGTGAT TTCATACCAA GACTTGGTGA AGTGTTTCAC ATTGATCATC 
CAGAGTCTAC AACGTGGTGA TATACAGCCA TGGCTCCATA GTGGAAGTAA CAGTTTACTA 
AGTAAGCTCA TTCATCAGTC TTATCATGGA ACCATGGACA CAGTTTCTCT CAGTGGGACT 
ATTCCAGTTC AAATGCTTTT GGAAATTGGT TTGGACAAAC TAAAGAAAGA TTATATCAGT 
TTTTTCATAG GTCAGGAACT TGCATCTTTG AATCATTTGG AATACTTCAT TGCTCCATCA 
GTAGATATAC AAGAACAGGT TTATCGTGTC CAAAAACTCC ACCATATTCT AGAAATATTA 
GTCAGTTGCA TGCCTTTCAT TAAATCTCAA CATGAACTCC TCTTTTCTTT AACACAGATC 
TGCATAAAGT ATTACAAACA AAATCCTCTT GATGAGCAAC ACATTTTTCA GCTGCCAGTC 
AGACCAACTG CTGTAAAGAA CTTATATCAA AGTGAGAAGC CACAGAAATG GAGAGTGGAA 
ATATATAGTG GTCAAAAGAA GATTAAGACA GTTTGGCAAC TGAGTGACAG CTCACCCATA 
GACCATCTGA ATTTTCACAA ACCTGATTTT TCGGAATTAA CACTAAACGG TAGCCTGGAA 
GAAAGGATAT TCTTTACTAA CATGGTTACC TGCAGCCAGG TGCATTTCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZWILCH ( NM_001287821.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274750.1 Homo sapiens zwilch kinetochore protein (ZWILCH), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10802
Clone ID Related Accession (Same CDS sequence)	NM_001287823.2 , NM_001287821.1 , NM_001287821.2 , NM_001287823.1 , NM_001287822.1
Accession Version	NM_001287821.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1530201600000
Organism	Homo sapiens(human)
Product	protein zwilch homolog isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC116913.6, BX640701.1 and BQ775697.1. Transcript Variant: This variant (2) uses an alternate splice site at an internal exon, compared to variant 1. This difference results in predicted translation initiation at a downstream in-frame start site through ribosomal re-initiation or leaky scanning. The encoded isoform (2) is shorter than isoform 1. Variants 2, 3, and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BX640701.1, SRR1803616.55349.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCACA ATCCTAATAT GACCCATTTG AAGATTAATC TGCCAGTTAC TGCCCTTCCT 
CCCCTTTGGG TAAGATGTGA CAGTTCAGAT CCTGAAGGTA CTTGTTGGCT AGGAGCTGAG 
CTTATCACAA CAAACAACAG CATTACAGGA ATTGTCTTAT ATGTGGTCAG TTGTAAAGCT 
GATAAAAATT ATTCTGTAAA TCTTGAAAAC CTAAAAAATT TACACAAGAA AAGACATCAC 
TTGTCTACTG TAACATCCAA AGGCTTTGCC CAGTATGAGC TCTTTAAGTC CTCTGCCTTG 
GATGATACAA TCACAGCATC ACAAACTGCG ATCGCTTTGG ATATTTCCTG GAGTCCTGTG 
GATGAGATTC TTCAAATCCC TCCACTCTCT TCAACTGCAA CTCTGAATAT TAAAGTGGAA 
TCAGGAGAGC CCAGAGGTCC TTTGAATCAT CTCTACAGAG AACTGAAATT TCTTCTTGTT 
TTGGCTGATG GTTTGAGGAC TGGTGTCACT GAATGGCTCG AGCCCCTGGA AGCAAAATCT 
GCTGTTGAAC TTGTTCAGGA ATTTCTGAAT GACTTAAATA AGCTGGATGG ATTTGGTGAT 
TCTACAAAAA AAGACACTGA GGTTGAGACC TTGAAGCATG ACACTGCTGC AGTCGATCGT 
TCCGTCAAGC GTCTTTTCAA AGTTCGGAGT GATCTTGATT TTGCTGAGCA ACTGTGGTGC 
AAAATGAGCA GTAGTGTGAT TTCATACCAA GACTTGGTGA AGTGTTTCAC ATTGATCATC 
CAGAGTCTAC AACGTGGTGA TATACAGCCA TGGCTCCATA GTGGAAGTAA CAGTTTACTA 
AGTAAGCTCA TTCATCAGTC TTATCATGGA ACCATGGACA CAGTTTCTCT CAGTGGGACT 
ATTCCAGTTC AAATGCTTTT GGAAATTGGT TTGGACAAAC TAAAGAAAGA TTATATCAGT 
TTTTTCATAG GTCAGGAACT TGCATCTTTG AATCATTTGG AATACTTCAT TGCTCCATCA 
GTAGATATAC AAGAACAGGT TTATCGTGTC CAAAAACTCC ACCATATTCT AGAAATATTA 
GTCAGTTGCA TGCCTTTCAT TAAATCTCAA CATGAACTCC TCTTTTCTTT AACACAGATC 
TGCATAAAGT ATTACAAACA AAATCCTCTT GATGAGCAAC ACATTTTTCA GCTGCCAGTC 
AGACCAACTG CTGTAAAGAA CTTATATCAA AGTGAGAAGC CACAGAAATG GAGAGTGGAA 
ATATATAGTG GTCAAAAGAA GATTAAGACA GTTTGGCAAC TGAGTGACAG CTCACCCATA 
GACCATCTGA ATTTTCACAA ACCTGATTTT TCGGAATTAA CACTAAACGG TAGCCTGGAA 
GAAAGGATAT TCTTTACTAA CATGGTTACC TGCAGCCAGG TGCATTTCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274750.1
CDS	694..2127
Translation	MAHNPNMTHLKINLPVTALPPLWVRCDSSDPEGTCWLGAELITTNNSITGIVLYVVSCKADKNYSVNLENLKNLHKKRHHLSTVTSKGFAQYELFKSSALDDTITASQTAIALDISWSPVDEILQIPPLSSTATLNIKVESGEPRGPLNHLYRELKFLLVLADGLRTGVTEWLEPLEAKSAVELVQEFLNDLNKLDGFGDSTKKDTEVETLKHDTAAVDRSVKRLFKVRSDLDFAEQLWCKMSSSVISYQDLVKCFTLIIQSLQRGDIQPWLHSGSNSLLSKLIHQSYHGTMDTVSLSGTIPVQMLLEIGLDKLKKDYISFFIGQELASLNHLEYFIAPSVDIQEQVYRVQKLHHILEILVSCMPFIKSQHELLFSLTQICIKYYKQNPLDEQHIFQLPVRPTAVKNLYQSEKPQKWRVEIYSGQKKIKTVWQLSDSSPIDHLNFHKPDFSELTLNGSLEERIFFTNMVTCSQVHFK

Target ORF information:

RefSeq Version	NM_001287821.1
Organism	Homo sapiens(human)
Definition	Homo sapiens zwilch kinetochore protein (ZWILCH), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287821.1

ORF Insert Sequence:

ATGGCTCACA ATCCTAATAT GACCCATTTG AAGATTAATC TGCCAGTTAC TGCCCTTCCT 
CCCCTTTGGG TAAGATGTGA CAGTTCAGAT CCTGAAGGTA CTTGTTGGCT AGGAGCTGAG 
CTTATCACAA CAAACAACAG CATTACAGGA ATTGTCTTAT ATGTGGTCAG TTGTAAAGCT 
GATAAAAATT ATTCTGTAAA TCTTGAAAAC CTAAAAAATT TACACAAGAA AAGACATCAC 
TTGTCTACTG TAACATCCAA AGGCTTTGCC CAGTATGAGC TCTTTAAGTC CTCTGCCTTG 
GATGATACAA TCACAGCATC ACAAACTGCG ATCGCTTTGG ATATTTCCTG GAGTCCTGTG 
GATGAGATTC TTCAAATCCC TCCACTCTCT TCAACTGCAA CTCTGAATAT TAAAGTGGAA 
TCAGGAGAGC CCAGAGGTCC TTTGAATCAT CTCTACAGAG AACTGAAATT TCTTCTTGTT 
TTGGCTGATG GTTTGAGGAC TGGTGTCACT GAATGGCTCG AGCCCCTGGA AGCAAAATCT 
GCTGTTGAAC TTGTTCAGGA ATTTCTGAAT GACTTAAATA AGCTGGATGG ATTTGGTGAT 
TCTACAAAAA AAGACACTGA GGTTGAGACC TTGAAGCATG ACACTGCTGC AGTCGATCGT 
TCCGTCAAGC GTCTTTTCAA AGTTCGGAGT GATCTTGATT TTGCTGAGCA ACTGTGGTGC 
AAAATGAGCA GTAGTGTGAT TTCATACCAA GACTTGGTGA AGTGTTTCAC ATTGATCATC 
CAGAGTCTAC AACGTGGTGA TATACAGCCA TGGCTCCATA GTGGAAGTAA CAGTTTACTA 
AGTAAGCTCA TTCATCAGTC TTATCATGGA ACCATGGACA CAGTTTCTCT CAGTGGGACT 
ATTCCAGTTC AAATGCTTTT GGAAATTGGT TTGGACAAAC TAAAGAAAGA TTATATCAGT 
TTTTTCATAG GTCAGGAACT TGCATCTTTG AATCATTTGG AATACTTCAT TGCTCCATCA 
GTAGATATAC AAGAACAGGT TTATCGTGTC CAAAAACTCC ACCATATTCT AGAAATATTA 
GTCAGTTGCA TGCCTTTCAT TAAATCTCAA CATGAACTCC TCTTTTCTTT AACACAGATC 
TGCATAAAGT ATTACAAACA AAATCCTCTT GATGAGCAAC ACATTTTTCA GCTGCCAGTC 
AGACCAACTG CTGTAAAGAA CTTATATCAA AGTGAGAAGC CACAGAAATG GAGAGTGGAA 
ATATATAGTG GTCAAAAGAA GATTAAGACA GTTTGGCAAC TGAGTGACAG CTCACCCATA 
GACCATCTGA ATTTTCACAA ACCTGATTTT TCGGAATTAA CACTAAACGG TAGCCTGGAA 
GAAAGGATAT TCTTTACTAA CATGGTTACC TGCAGCCAGG TGCATTTCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZWILCH ( NM_001287821.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274750.1 Homo sapiens zwilch kinetochore protein (ZWILCH), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10802
Clone ID Related Accession (Same CDS sequence)	NM_001287823.2 , NM_001287821.1 , NM_001287821.2 , NM_001287823.1 , NM_001287822.1
Accession Version	NM_001287821.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1559145600000
Organism	Homo sapiens(human)
Product	protein zwilch homolog isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX640701.1, AC116913.6 and BQ775697.1. On May 31, 2019 this sequence version replaced NM_001287821.1. Transcript Variant: This variant (2) uses an alternate splice site at an internal exon, compared to variant 1. This difference results in predicted translation initiation at a downstream in-frame start site through ribosomal re-initiation or leaky scanning. The encoded isoform (2) is shorter than isoform 1. Variants 2, 3, and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.158940.1, SRR1803617.133470.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCACA ATCCTAATAT GACCCATTTG AAGATTAATC TGCCAGTTAC TGCCCTTCCT 
CCCCTTTGGG TAAGATGTGA CAGTTCAGAT CCTGAAGGTA CTTGTTGGCT AGGAGCTGAG 
CTTATCACAA CAAACAACAG CATTACAGGA ATTGTCTTAT ATGTGGTCAG TTGTAAAGCT 
GATAAAAATT ATTCTGTAAA TCTTGAAAAC CTAAAAAATT TACACAAGAA AAGACATCAC 
TTGTCTACTG TAACATCCAA AGGCTTTGCC CAGTATGAGC TCTTTAAGTC CTCTGCCTTG 
GATGATACAA TCACAGCATC ACAAACTGCG ATCGCTTTGG ATATTTCCTG GAGTCCTGTG 
GATGAGATTC TTCAAATCCC TCCACTCTCT TCAACTGCAA CTCTGAATAT TAAAGTGGAA 
TCAGGAGAGC CCAGAGGTCC TTTGAATCAT CTCTACAGAG AACTGAAATT TCTTCTTGTT 
TTGGCTGATG GTTTGAGGAC TGGTGTCACT GAATGGCTCG AGCCCCTGGA AGCAAAATCT 
GCTGTTGAAC TTGTTCAGGA ATTTCTGAAT GACTTAAATA AGCTGGATGG ATTTGGTGAT 
TCTACAAAAA AAGACACTGA GGTTGAGACC TTGAAGCATG ACACTGCTGC AGTCGATCGT 
TCCGTCAAGC GTCTTTTCAA AGTTCGGAGT GATCTTGATT TTGCTGAGCA ACTGTGGTGC 
AAAATGAGCA GTAGTGTGAT TTCATACCAA GACTTGGTGA AGTGTTTCAC ATTGATCATC 
CAGAGTCTAC AACGTGGTGA TATACAGCCA TGGCTCCATA GTGGAAGTAA CAGTTTACTA 
AGTAAGCTCA TTCATCAGTC TTATCATGGA ACCATGGACA CAGTTTCTCT CAGTGGGACT 
ATTCCAGTTC AAATGCTTTT GGAAATTGGT TTGGACAAAC TAAAGAAAGA TTATATCAGT 
TTTTTCATAG GTCAGGAACT TGCATCTTTG AATCATTTGG AATACTTCAT TGCTCCATCA 
GTAGATATAC AAGAACAGGT TTATCGTGTC CAAAAACTCC ACCATATTCT AGAAATATTA 
GTCAGTTGCA TGCCTTTCAT TAAATCTCAA CATGAACTCC TCTTTTCTTT AACACAGATC 
TGCATAAAGT ATTACAAACA AAATCCTCTT GATGAGCAAC ACATTTTTCA GCTGCCAGTC 
AGACCAACTG CTGTAAAGAA CTTATATCAA AGTGAGAAGC CACAGAAATG GAGAGTGGAA 
ATATATAGTG GTCAAAAGAA GATTAAGACA GTTTGGCAAC TGAGTGACAG CTCACCCATA 
GACCATCTGA ATTTTCACAA ACCTGATTTT TCGGAATTAA CACTAAACGG TAGCCTGGAA 
GAAAGGATAT TCTTTACTAA CATGGTTACC TGCAGCCAGG TGCATTTCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274750.1
CDS	451..1884
Translation	MAHNPNMTHLKINLPVTALPPLWVRCDSSDPEGTCWLGAELITTNNSITGIVLYVVSCKADKNYSVNLENLKNLHKKRHHLSTVTSKGFAQYELFKSSALDDTITASQTAIALDISWSPVDEILQIPPLSSTATLNIKVESGEPRGPLNHLYRELKFLLVLADGLRTGVTEWLEPLEAKSAVELVQEFLNDLNKLDGFGDSTKKDTEVETLKHDTAAVDRSVKRLFKVRSDLDFAEQLWCKMSSSVISYQDLVKCFTLIIQSLQRGDIQPWLHSGSNSLLSKLIHQSYHGTMDTVSLSGTIPVQMLLEIGLDKLKKDYISFFIGQELASLNHLEYFIAPSVDIQEQVYRVQKLHHILEILVSCMPFIKSQHELLFSLTQICIKYYKQNPLDEQHIFQLPVRPTAVKNLYQSEKPQKWRVEIYSGQKKIKTVWQLSDSSPIDHLNFHKPDFSELTLNGSLEERIFFTNMVTCSQVHFK

Target ORF information:

RefSeq Version	NM_001287821.2
Organism	Homo sapiens(human)
Definition	Homo sapiens zwilch kinetochore protein (ZWILCH), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287821.2

ORF Insert Sequence:

ATGGCTCACA ATCCTAATAT GACCCATTTG AAGATTAATC TGCCAGTTAC TGCCCTTCCT 
CCCCTTTGGG TAAGATGTGA CAGTTCAGAT CCTGAAGGTA CTTGTTGGCT AGGAGCTGAG 
CTTATCACAA CAAACAACAG CATTACAGGA ATTGTCTTAT ATGTGGTCAG TTGTAAAGCT 
GATAAAAATT ATTCTGTAAA TCTTGAAAAC CTAAAAAATT TACACAAGAA AAGACATCAC 
TTGTCTACTG TAACATCCAA AGGCTTTGCC CAGTATGAGC TCTTTAAGTC CTCTGCCTTG 
GATGATACAA TCACAGCATC ACAAACTGCG ATCGCTTTGG ATATTTCCTG GAGTCCTGTG 
GATGAGATTC TTCAAATCCC TCCACTCTCT TCAACTGCAA CTCTGAATAT TAAAGTGGAA 
TCAGGAGAGC CCAGAGGTCC TTTGAATCAT CTCTACAGAG AACTGAAATT TCTTCTTGTT 
TTGGCTGATG GTTTGAGGAC TGGTGTCACT GAATGGCTCG AGCCCCTGGA AGCAAAATCT 
GCTGTTGAAC TTGTTCAGGA ATTTCTGAAT GACTTAAATA AGCTGGATGG ATTTGGTGAT 
TCTACAAAAA AAGACACTGA GGTTGAGACC TTGAAGCATG ACACTGCTGC AGTCGATCGT 
TCCGTCAAGC GTCTTTTCAA AGTTCGGAGT GATCTTGATT TTGCTGAGCA ACTGTGGTGC 
AAAATGAGCA GTAGTGTGAT TTCATACCAA GACTTGGTGA AGTGTTTCAC ATTGATCATC 
CAGAGTCTAC AACGTGGTGA TATACAGCCA TGGCTCCATA GTGGAAGTAA CAGTTTACTA 
AGTAAGCTCA TTCATCAGTC TTATCATGGA ACCATGGACA CAGTTTCTCT CAGTGGGACT 
ATTCCAGTTC AAATGCTTTT GGAAATTGGT TTGGACAAAC TAAAGAAAGA TTATATCAGT 
TTTTTCATAG GTCAGGAACT TGCATCTTTG AATCATTTGG AATACTTCAT TGCTCCATCA 
GTAGATATAC AAGAACAGGT TTATCGTGTC CAAAAACTCC ACCATATTCT AGAAATATTA 
GTCAGTTGCA TGCCTTTCAT TAAATCTCAA CATGAACTCC TCTTTTCTTT AACACAGATC 
TGCATAAAGT ATTACAAACA AAATCCTCTT GATGAGCAAC ACATTTTTCA GCTGCCAGTC 
AGACCAACTG CTGTAAAGAA CTTATATCAA AGTGAGAAGC CACAGAAATG GAGAGTGGAA 
ATATATAGTG GTCAAAAGAA GATTAAGACA GTTTGGCAAC TGAGTGACAG CTCACCCATA 
GACCATCTGA ATTTTCACAA ACCTGATTTT TCGGAATTAA CACTAAACGG TAGCCTGGAA 
GAAAGGATAT TCTTTACTAA CATGGTTACC TGCAGCCAGG TGCATTTCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZWILCH ( NM_001287823.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274752.1 Homo sapiens zwilch kinetochore protein (ZWILCH), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu10802
Clone ID Related Accession (Same CDS sequence)	NM_001287823.2 , NM_001287821.1 , NM_001287821.2 , NM_001287823.1 , NM_001287822.1
Accession Version	NM_001287823.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1530201600000
Organism	Homo sapiens(human)
Product	protein zwilch homolog isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC116913.6, AK291048.1, BC036900.1 and BQ775697.1. Transcript Variant: This variant (4) lacks an internal exon, compared to variant 1. This difference results in predicted translation initiation at a downstream in-frame start site through ribosomal re-initiation or leaky scanning. The encoded isoform (2) is shorter than isoform 1. Variants 2, 3, and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.158729.1, SRR1803613.76391.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCACA ATCCTAATAT GACCCATTTG AAGATTAATC TGCCAGTTAC TGCCCTTCCT 
CCCCTTTGGG TAAGATGTGA CAGTTCAGAT CCTGAAGGTA CTTGTTGGCT AGGAGCTGAG 
CTTATCACAA CAAACAACAG CATTACAGGA ATTGTCTTAT ATGTGGTCAG TTGTAAAGCT 
GATAAAAATT ATTCTGTAAA TCTTGAAAAC CTAAAAAATT TACACAAGAA AAGACATCAC 
TTGTCTACTG TAACATCCAA AGGCTTTGCC CAGTATGAGC TCTTTAAGTC CTCTGCCTTG 
GATGATACAA TCACAGCATC ACAAACTGCG ATCGCTTTGG ATATTTCCTG GAGTCCTGTG 
GATGAGATTC TTCAAATCCC TCCACTCTCT TCAACTGCAA CTCTGAATAT TAAAGTGGAA 
TCAGGAGAGC CCAGAGGTCC TTTGAATCAT CTCTACAGAG AACTGAAATT TCTTCTTGTT 
TTGGCTGATG GTTTGAGGAC TGGTGTCACT GAATGGCTCG AGCCCCTGGA AGCAAAATCT 
GCTGTTGAAC TTGTTCAGGA ATTTCTGAAT GACTTAAATA AGCTGGATGG ATTTGGTGAT 
TCTACAAAAA AAGACACTGA GGTTGAGACC TTGAAGCATG ACACTGCTGC AGTCGATCGT 
TCCGTCAAGC GTCTTTTCAA AGTTCGGAGT GATCTTGATT TTGCTGAGCA ACTGTGGTGC 
AAAATGAGCA GTAGTGTGAT TTCATACCAA GACTTGGTGA AGTGTTTCAC ATTGATCATC 
CAGAGTCTAC AACGTGGTGA TATACAGCCA TGGCTCCATA GTGGAAGTAA CAGTTTACTA 
AGTAAGCTCA TTCATCAGTC TTATCATGGA ACCATGGACA CAGTTTCTCT CAGTGGGACT 
ATTCCAGTTC AAATGCTTTT GGAAATTGGT TTGGACAAAC TAAAGAAAGA TTATATCAGT 
TTTTTCATAG GTCAGGAACT TGCATCTTTG AATCATTTGG AATACTTCAT TGCTCCATCA 
GTAGATATAC AAGAACAGGT TTATCGTGTC CAAAAACTCC ACCATATTCT AGAAATATTA 
GTCAGTTGCA TGCCTTTCAT TAAATCTCAA CATGAACTCC TCTTTTCTTT AACACAGATC 
TGCATAAAGT ATTACAAACA AAATCCTCTT GATGAGCAAC ACATTTTTCA GCTGCCAGTC 
AGACCAACTG CTGTAAAGAA CTTATATCAA AGTGAGAAGC CACAGAAATG GAGAGTGGAA 
ATATATAGTG GTCAAAAGAA GATTAAGACA GTTTGGCAAC TGAGTGACAG CTCACCCATA 
GACCATCTGA ATTTTCACAA ACCTGATTTT TCGGAATTAA CACTAAACGG TAGCCTGGAA 
GAAAGGATAT TCTTTACTAA CATGGTTACC TGCAGCCAGG TGCATTTCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274752.1
CDS	547..1980
Translation	MAHNPNMTHLKINLPVTALPPLWVRCDSSDPEGTCWLGAELITTNNSITGIVLYVVSCKADKNYSVNLENLKNLHKKRHHLSTVTSKGFAQYELFKSSALDDTITASQTAIALDISWSPVDEILQIPPLSSTATLNIKVESGEPRGPLNHLYRELKFLLVLADGLRTGVTEWLEPLEAKSAVELVQEFLNDLNKLDGFGDSTKKDTEVETLKHDTAAVDRSVKRLFKVRSDLDFAEQLWCKMSSSVISYQDLVKCFTLIIQSLQRGDIQPWLHSGSNSLLSKLIHQSYHGTMDTVSLSGTIPVQMLLEIGLDKLKKDYISFFIGQELASLNHLEYFIAPSVDIQEQVYRVQKLHHILEILVSCMPFIKSQHELLFSLTQICIKYYKQNPLDEQHIFQLPVRPTAVKNLYQSEKPQKWRVEIYSGQKKIKTVWQLSDSSPIDHLNFHKPDFSELTLNGSLEERIFFTNMVTCSQVHFK

Target ORF information:

RefSeq Version	NM_001287823.1
Organism	Homo sapiens(human)
Definition	Homo sapiens zwilch kinetochore protein (ZWILCH), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287823.1

ORF Insert Sequence:

ATGGCTCACA ATCCTAATAT GACCCATTTG AAGATTAATC TGCCAGTTAC TGCCCTTCCT 
CCCCTTTGGG TAAGATGTGA CAGTTCAGAT CCTGAAGGTA CTTGTTGGCT AGGAGCTGAG 
CTTATCACAA CAAACAACAG CATTACAGGA ATTGTCTTAT ATGTGGTCAG TTGTAAAGCT 
GATAAAAATT ATTCTGTAAA TCTTGAAAAC CTAAAAAATT TACACAAGAA AAGACATCAC 
TTGTCTACTG TAACATCCAA AGGCTTTGCC CAGTATGAGC TCTTTAAGTC CTCTGCCTTG 
GATGATACAA TCACAGCATC ACAAACTGCG ATCGCTTTGG ATATTTCCTG GAGTCCTGTG 
GATGAGATTC TTCAAATCCC TCCACTCTCT TCAACTGCAA CTCTGAATAT TAAAGTGGAA 
TCAGGAGAGC CCAGAGGTCC TTTGAATCAT CTCTACAGAG AACTGAAATT TCTTCTTGTT 
TTGGCTGATG GTTTGAGGAC TGGTGTCACT GAATGGCTCG AGCCCCTGGA AGCAAAATCT 
GCTGTTGAAC TTGTTCAGGA ATTTCTGAAT GACTTAAATA AGCTGGATGG ATTTGGTGAT 
TCTACAAAAA AAGACACTGA GGTTGAGACC TTGAAGCATG ACACTGCTGC AGTCGATCGT 
TCCGTCAAGC GTCTTTTCAA AGTTCGGAGT GATCTTGATT TTGCTGAGCA ACTGTGGTGC 
AAAATGAGCA GTAGTGTGAT TTCATACCAA GACTTGGTGA AGTGTTTCAC ATTGATCATC 
CAGAGTCTAC AACGTGGTGA TATACAGCCA TGGCTCCATA GTGGAAGTAA CAGTTTACTA 
AGTAAGCTCA TTCATCAGTC TTATCATGGA ACCATGGACA CAGTTTCTCT CAGTGGGACT 
ATTCCAGTTC AAATGCTTTT GGAAATTGGT TTGGACAAAC TAAAGAAAGA TTATATCAGT 
TTTTTCATAG GTCAGGAACT TGCATCTTTG AATCATTTGG AATACTTCAT TGCTCCATCA 
GTAGATATAC AAGAACAGGT TTATCGTGTC CAAAAACTCC ACCATATTCT AGAAATATTA 
GTCAGTTGCA TGCCTTTCAT TAAATCTCAA CATGAACTCC TCTTTTCTTT AACACAGATC 
TGCATAAAGT ATTACAAACA AAATCCTCTT GATGAGCAAC ACATTTTTCA GCTGCCAGTC 
AGACCAACTG CTGTAAAGAA CTTATATCAA AGTGAGAAGC CACAGAAATG GAGAGTGGAA 
ATATATAGTG GTCAAAAGAA GATTAAGACA GTTTGGCAAC TGAGTGACAG CTCACCCATA 
GACCATCTGA ATTTTCACAA ACCTGATTTT TCGGAATTAA CACTAAACGG TAGCCTGGAA 
GAAAGGATAT TCTTTACTAA CATGGTTACC TGCAGCCAGG TGCATTTCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZWILCH ( NM_001287822.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274751.1 Homo sapiens zwilch kinetochore protein (ZWILCH), transcript variant 3, mRNA.

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CloneID	OHu10802
Clone ID Related Accession (Same CDS sequence)	NM_001287823.2 , NM_001287821.1 , NM_001287821.2 , NM_001287823.1 , NM_001287822.1
Accession Version	NM_001287822.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1530201600000
Organism	Homo sapiens(human)
Product	protein zwilch homolog isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC116913.6, AK122787.1, BC036900.1 and BQ775697.1. Transcript Variant: This variant (3) uses an alternate splice site at an internal exon and lacks an exon, compared to variant 1. These differences result in predicted translation initiation at a downstream in-frame start site through ribosomal re-initiation or leaky scanning. The encoded isoform (2) is shorter than isoform 1. Variants 2, 3, and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK122787.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151119 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCACA ATCCTAATAT GACCCATTTG AAGATTAATC TGCCAGTTAC TGCCCTTCCT 
CCCCTTTGGG TAAGATGTGA CAGTTCAGAT CCTGAAGGTA CTTGTTGGCT AGGAGCTGAG 
CTTATCACAA CAAACAACAG CATTACAGGA ATTGTCTTAT ATGTGGTCAG TTGTAAAGCT 
GATAAAAATT ATTCTGTAAA TCTTGAAAAC CTAAAAAATT TACACAAGAA AAGACATCAC 
TTGTCTACTG TAACATCCAA AGGCTTTGCC CAGTATGAGC TCTTTAAGTC CTCTGCCTTG 
GATGATACAA TCACAGCATC ACAAACTGCG ATCGCTTTGG ATATTTCCTG GAGTCCTGTG 
GATGAGATTC TTCAAATCCC TCCACTCTCT TCAACTGCAA CTCTGAATAT TAAAGTGGAA 
TCAGGAGAGC CCAGAGGTCC TTTGAATCAT CTCTACAGAG AACTGAAATT TCTTCTTGTT 
TTGGCTGATG GTTTGAGGAC TGGTGTCACT GAATGGCTCG AGCCCCTGGA AGCAAAATCT 
GCTGTTGAAC TTGTTCAGGA ATTTCTGAAT GACTTAAATA AGCTGGATGG ATTTGGTGAT 
TCTACAAAAA AAGACACTGA GGTTGAGACC TTGAAGCATG ACACTGCTGC AGTCGATCGT 
TCCGTCAAGC GTCTTTTCAA AGTTCGGAGT GATCTTGATT TTGCTGAGCA ACTGTGGTGC 
AAAATGAGCA GTAGTGTGAT TTCATACCAA GACTTGGTGA AGTGTTTCAC ATTGATCATC 
CAGAGTCTAC AACGTGGTGA TATACAGCCA TGGCTCCATA GTGGAAGTAA CAGTTTACTA 
AGTAAGCTCA TTCATCAGTC TTATCATGGA ACCATGGACA CAGTTTCTCT CAGTGGGACT 
ATTCCAGTTC AAATGCTTTT GGAAATTGGT TTGGACAAAC TAAAGAAAGA TTATATCAGT 
TTTTTCATAG GTCAGGAACT TGCATCTTTG AATCATTTGG AATACTTCAT TGCTCCATCA 
GTAGATATAC AAGAACAGGT TTATCGTGTC CAAAAACTCC ACCATATTCT AGAAATATTA 
GTCAGTTGCA TGCCTTTCAT TAAATCTCAA CATGAACTCC TCTTTTCTTT AACACAGATC 
TGCATAAAGT ATTACAAACA AAATCCTCTT GATGAGCAAC ACATTTTTCA GCTGCCAGTC 
AGACCAACTG CTGTAAAGAA CTTATATCAA AGTGAGAAGC CACAGAAATG GAGAGTGGAA 
ATATATAGTG GTCAAAAGAA GATTAAGACA GTTTGGCAAC TGAGTGACAG CTCACCCATA 
GACCATCTGA ATTTTCACAA ACCTGATTTT TCGGAATTAA CACTAAACGG TAGCCTGGAA 
GAAAGGATAT TCTTTACTAA CATGGTTACC TGCAGCCAGG TGCATTTCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274751.1
CDS	598..2031
Translation	MAHNPNMTHLKINLPVTALPPLWVRCDSSDPEGTCWLGAELITTNNSITGIVLYVVSCKADKNYSVNLENLKNLHKKRHHLSTVTSKGFAQYELFKSSALDDTITASQTAIALDISWSPVDEILQIPPLSSTATLNIKVESGEPRGPLNHLYRELKFLLVLADGLRTGVTEWLEPLEAKSAVELVQEFLNDLNKLDGFGDSTKKDTEVETLKHDTAAVDRSVKRLFKVRSDLDFAEQLWCKMSSSVISYQDLVKCFTLIIQSLQRGDIQPWLHSGSNSLLSKLIHQSYHGTMDTVSLSGTIPVQMLLEIGLDKLKKDYISFFIGQELASLNHLEYFIAPSVDIQEQVYRVQKLHHILEILVSCMPFIKSQHELLFSLTQICIKYYKQNPLDEQHIFQLPVRPTAVKNLYQSEKPQKWRVEIYSGQKKIKTVWQLSDSSPIDHLNFHKPDFSELTLNGSLEERIFFTNMVTCSQVHFK

Target ORF information:

RefSeq Version	NM_001287822.1
Organism	Homo sapiens(human)
Definition	Homo sapiens zwilch kinetochore protein (ZWILCH), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287822.1

ORF Insert Sequence:

ATGGCTCACA ATCCTAATAT GACCCATTTG AAGATTAATC TGCCAGTTAC TGCCCTTCCT 
CCCCTTTGGG TAAGATGTGA CAGTTCAGAT CCTGAAGGTA CTTGTTGGCT AGGAGCTGAG 
CTTATCACAA CAAACAACAG CATTACAGGA ATTGTCTTAT ATGTGGTCAG TTGTAAAGCT 
GATAAAAATT ATTCTGTAAA TCTTGAAAAC CTAAAAAATT TACACAAGAA AAGACATCAC 
TTGTCTACTG TAACATCCAA AGGCTTTGCC CAGTATGAGC TCTTTAAGTC CTCTGCCTTG 
GATGATACAA TCACAGCATC ACAAACTGCG ATCGCTTTGG ATATTTCCTG GAGTCCTGTG 
GATGAGATTC TTCAAATCCC TCCACTCTCT TCAACTGCAA CTCTGAATAT TAAAGTGGAA 
TCAGGAGAGC CCAGAGGTCC TTTGAATCAT CTCTACAGAG AACTGAAATT TCTTCTTGTT 
TTGGCTGATG GTTTGAGGAC TGGTGTCACT GAATGGCTCG AGCCCCTGGA AGCAAAATCT 
GCTGTTGAAC TTGTTCAGGA ATTTCTGAAT GACTTAAATA AGCTGGATGG ATTTGGTGAT 
TCTACAAAAA AAGACACTGA GGTTGAGACC TTGAAGCATG ACACTGCTGC AGTCGATCGT 
TCCGTCAAGC GTCTTTTCAA AGTTCGGAGT GATCTTGATT TTGCTGAGCA ACTGTGGTGC 
AAAATGAGCA GTAGTGTGAT TTCATACCAA GACTTGGTGA AGTGTTTCAC ATTGATCATC 
CAGAGTCTAC AACGTGGTGA TATACAGCCA TGGCTCCATA GTGGAAGTAA CAGTTTACTA 
AGTAAGCTCA TTCATCAGTC TTATCATGGA ACCATGGACA CAGTTTCTCT CAGTGGGACT 
ATTCCAGTTC AAATGCTTTT GGAAATTGGT TTGGACAAAC TAAAGAAAGA TTATATCAGT 
TTTTTCATAG GTCAGGAACT TGCATCTTTG AATCATTTGG AATACTTCAT TGCTCCATCA 
GTAGATATAC AAGAACAGGT TTATCGTGTC CAAAAACTCC ACCATATTCT AGAAATATTA 
GTCAGTTGCA TGCCTTTCAT TAAATCTCAA CATGAACTCC TCTTTTCTTT AACACAGATC 
TGCATAAAGT ATTACAAACA AAATCCTCTT GATGAGCAAC ACATTTTTCA GCTGCCAGTC 
AGACCAACTG CTGTAAAGAA CTTATATCAA AGTGAGAAGC CACAGAAATG GAGAGTGGAA 
ATATATAGTG GTCAAAAGAA GATTAAGACA GTTTGGCAAC TGAGTGACAG CTCACCCATA 
GACCATCTGA ATTTTCACAA ACCTGATTTT TCGGAATTAA CACTAAACGG TAGCCTGGAA 
GAAAGGATAT TCTTTACTAA CATGGTTACC TGCAGCCAGG TGCATTTCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Investigation of genetic susceptibility factors for human longevity - a targeted nonsynonymous SNP study.
Mutation research694(1-2)13-9(2010 Dec)
Flachsbart F,Franke A,Kleindorp R,Caliebe A,Blanché H,Schreiber S,Nebel A

Structural analysis of the RZZ complex reveals common ancestry with multisubunit vesicle tethering machinery.
Structure (London, England : 1993)18(5)616-26(2010 May)
Civril F,Wehenkel A,Giorgi FM,Santaguida S,Di Fonzo A,Grigorean G,Ciccarelli FD,Musacchio A

Single nucleotide polymorphisms in chromosomal instability genes and risk and clinical outcome of breast cancer: a Swedish prospective case-control study.
European journal of cancer (Oxford, England : 1990)45(3)435-42(2009 Feb)
Brendle A,Brandt A,Johansson R,Enquist K,Hallmans G,Hemminki K,Lenner P,Försti A

ZW10 links mitotic checkpoint signaling to the structural kinetochore.
The Journal of cell biology169(1)49-60(2005 Apr)
Kops GJ,Kim Y,Weaver BA,Mao Y,McLeod I,Yates JR 3rd,Tagaya M,Cleveland DW

Zwilch, a new component of the ZW10/ROD complex required for kinetochore functions.
Molecular biology of the cell14(4)1379-91(2003 Apr)
Williams BC,Li Z,Liu S,Williams EV,Leung G,Yen TJ,Goldberg ML

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ZWILCH cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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