MED18 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED18
Entrez Gene ID	54797
Full Name	mediator complex subunit 18
Synonyms	SRB5,p28b
General protein information	Preferred Names mediator complex subunit 18 Names mediator of RNA polymerase II transcription subunit 18 TRAP/mediator complex subunit p28b mediator of RNA polymerase II transcription, subunit 18 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p35.3
Summary	MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II (Sato et al., 2003 [PubMed 12584197]).[supplied by OMIM, Oct 2008].
Disorder MIM:	612384

mRNA and Protein(s)

mRNA	Protein	Name
NM_001127350.1	NP_001120822.1	mediator of RNA polymerase II transcription subunit 18
XM_005245914.3	XP_005245971.1	mediator of RNA polymerase II transcription subunit 18 isoform X1
NM_017638.3	NP_060108.2	mediator of RNA polymerase II transcription subunit 18
NM_017638.2	NP_060108.2	mediator of RNA polymerase II transcription subunit 18
XM_005245914.4	XP_005245971.1	mediator of RNA polymerase II transcription subunit 18 isoform X1
NM_001127350.2	NP_001120822.1	mediator of RNA polymerase II transcription subunit 18

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	MED18	NP_569948.3
Rattus norvegicus (Norway rat)	LOC682988	NP_001108509.1
Caenorhabditis elegans (roundworm)	mdt-18	NP_491869.1
Anopheles gambiae (African malaria mosquito)	MED18_ANOGA	XP_309758.4
Macaca mulatta (Rhesus monkey)	MED18	XP_002802314.1
Mus musculus (house mouse)	Med18	NP_080315.1
Danio rerio (zebrafish)	med18	NP_956629.1
Bos taurus (cattle)	MED18	NP_001069703.1
Xenopus tropicalis (tropical clawed frog)	med18	NP_001017086.1
Gallus gallus (chicken)	MED18	XP_003643008.1
Homo sapiens (human)	MED18	NP_001120822.1
Pan troglodytes (chimpanzee)	MED18	XP_001152339.1
Canis lupus familiaris (dog)	MED18	XP_544460.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Mediator MED18 subunit plays a negative role in transcription via the CDK/cyclin module.
Kumafuji M, Umemura H, Furumoto T, Fukasawa R, Tanaka A, Ohkuma Y
Genes to cells : devoted to molecular & cellular mechanisms19(7)582-93(2014 Jul)

Subunit architecture and functional modular rearrangements of the transcriptional mediator complex.
Tsai KL, Tomomori-Sato C, Sato S, Conaway RC, Conaway JW, Asturias FJ
Cell157(6)1430-44(2014 Jun)

A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology.
Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC
Molecular cell14(5)685-91(2004 Jun)

A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2.
Tomomori-Sato C, Sato S, Parmely TJ, Banks CA, Sorokina I, Florens L, Zybailov B, Washburn MP, Brower CS, Conaway RC, Conaway JW
The Journal of biological chemistry279(7)5846-51(2004 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MED18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu24315	NM_017638.3 Latest version!	Homo sapiens mediator complex subunit 18 (MED18), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu24315	NM_001127350.2 Latest version!	Homo sapiens mediator complex subunit 18 (MED18), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu24315	XM_005245914.4 Latest version!	Homo sapiens mediator complex subunit 18 (MED18), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
View Old Accession Versions >>
OHu24315	NM_017638.2	Homo sapiens mediator complex subunit 18 (MED18), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu24315	XM_005245914.3	Homo sapiens mediator complex subunit 18 (MED18), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu24315	NM_001127350.1	Homo sapiens mediator complex subunit 18 (MED18), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED18 ( NM_001127350.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001120822.1 Homo sapiens mediator complex subunit 18 (MED18), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu24315
Clone ID Related Accession (Same CDS sequence)	NM_001127350.1 , XM_005245914.3 , NM_017638.3 , NM_017638.2 , XM_005245914.4 , NM_001127350.2
Accession Version	NM_001127350.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1529683200000
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 18
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG752399.1 and AL353622.33. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.129010.1, SRR1163657.490968.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCAC CTCCAGTCAC CATGATGCCT GTCACTGGGG GCACCATTAA CATGATGGAG 
TACCTGTTGC AGGGAAGTGT TTTAGATCAC AGTTTGGAAA GCCTCATCCA CCGCCTTCGT 
GGTTTGTGTG ACAACATGGA ACCTGAGACT TTCCTTGACC ATGAGATGGT ATTCCTCCTT 
AAGGGCCAGC AAGCCAGCCC ATTTGTTCTC AGGGCCCGAC GCTCTATGGA CAGGGCAGGG 
GCACCCTGGC ATCTGCGCTA CCTGGGACAG CCAGAAATGG GAGACAAGAA CCGCCATGCC 
CTGGTGCGAA ACTGCGTGGA CATTGCCACA TCTGAGAACC TCACCGACTT CTTGATGGAA 
ATGGGCTTCC GCATGGACCA TGAGTTTGTT GCTAAGGGAC ATTTGTTCCG TAAGGGCATC 
ATGAAGATTA TGGTGTACAA GATTTTCCGC ATCCTGGTGC CAGGGAACAC AGACAGCACT 
GAGGCCTTGT CACTCTCCTA TCTCGTGGAA TTAAGTGTGG TAGCACCCGC TGGGCAGGAC 
ATGGTCTCTG ATGACATGAA GAACTTCGCA GAACAGCTAA AACCTCTGGT TCACCTAGAG 
AAAATAGACC CCAAGAGGCT CATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120822.1
CDS	190..816
Translation	MEAPPVTMMPVTGGTINMMEYLLQGSVLDHSLESLIHRLRGLCDNMEPETFLDHEMVFLLKGQQASPFVLRARRSMDRAGAPWHLRYLGQPEMGDKNRHALVRNCVDIATSENLTDFLMEMGFRMDHEFVAKGHLFRKGIMKIMVYKIFRILVPGNTDSTEALSLSYLVELSVVAPAGQDMVSDDMKNFAEQLKPLVHLEKIDPKRLM

Target ORF information:

RefSeq Version	NM_001127350.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 18 (MED18), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127350.1

ORF Insert Sequence:

ATGGAGGCAC CTCCAGTCAC CATGATGCCT GTCACTGGGG GCACCATTAA CATGATGGAG 
TACCTGTTGC AGGGAAGTGT TTTAGATCAC AGTTTGGAAA GCCTCATCCA CCGCCTTCGT 
GGTTTGTGTG ACAACATGGA ACCTGAGACT TTCCTTGACC ATGAGATGGT ATTCCTCCTT 
AAGGGCCAGC AAGCCAGCCC ATTTGTTCTC AGGGCCCGAC GCTCTATGGA CAGGGCAGGG 
GCACCCTGGC ATCTGCGCTA CCTGGGACAG CCAGAAATGG GAGACAAGAA CCGCCATGCC 
CTGGTGCGAA ACTGCGTGGA CATTGCCACA TCTGAGAACC TCACCGACTT CTTGATGGAA 
ATGGGCTTCC GCATGGACCA TGAGTTTGTT GCTAAGGGAC ATTTGTTCCG TAAGGGCATC 
ATGAAGATTA TGGTGTACAA GATTTTCCGC ATCCTGGTGC CAGGGAACAC AGACAGCACT 
GAGGCCTTGT CACTCTCCTA TCTCGTGGAA TTAAGTGTGG TAGCACCCGC TGGGCAGGAC 
ATGGTCTCTG ATGACATGAA GAACTTCGCA GAACAGCTAA AACCTCTGGT TCACCTAGAG 
AAAATAGACC CCAAGAGGCT CATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED18 ( NM_017638.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060108.2 Homo sapiens mediator complex subunit 18 (MED18), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu24315
Clone ID Related Accession (Same CDS sequence)	NM_001127350.1 , XM_005245914.3 , NM_017638.3 , NM_017638.2 , XM_005245914.4 , NM_001127350.2
Accession Version	NM_017638.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570550400000
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 18
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK000052.1 and AL353622.33. On Nov 22, 2018 this sequence version replaced NM_017638.2. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.176063.1, SRR1803613.139732.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000373842.9/ ENSP00000362948.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGGCAC CTCCAGTCAC CATGATGCCT GTCACTGGGG GCACCATTAA CATGATGGAG 
TACCTGTTGC AGGGAAGTGT TTTAGATCAC AGTTTGGAAA GCCTCATCCA CCGCCTTCGT 
GGTTTGTGTG ACAACATGGA ACCTGAGACT TTCCTTGACC ATGAGATGGT ATTCCTCCTT 
AAGGGCCAGC AAGCCAGCCC ATTTGTTCTC AGGGCCCGAC GCTCTATGGA CAGGGCAGGG 
GCACCCTGGC ATCTGCGCTA CCTGGGACAG CCAGAAATGG GAGACAAGAA CCGCCATGCC 
CTGGTGCGAA ACTGCGTGGA CATTGCCACA TCTGAGAACC TCACCGACTT CTTGATGGAA 
ATGGGCTTCC GCATGGACCA TGAGTTTGTT GCTAAGGGAC ATTTGTTCCG TAAGGGCATC 
ATGAAGATTA TGGTGTACAA GATTTTCCGC ATCCTGGTGC CAGGGAACAC AGACAGCACT 
GAGGCCTTGT CACTCTCCTA TCTCGTGGAA TTAAGTGTGG TAGCACCCGC TGGGCAGGAC 
ATGGTCTCTG ATGACATGAA GAACTTCGCA GAACAGCTAA AACCTCTGGT TCACCTAGAG 
AAAATAGACC CCAAGAGGCT CATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060108.2
CDS	208..834
Translation	MEAPPVTMMPVTGGTINMMEYLLQGSVLDHSLESLIHRLRGLCDNMEPETFLDHEMVFLLKGQQASPFVLRARRSMDRAGAPWHLRYLGQPEMGDKNRHALVRNCVDIATSENLTDFLMEMGFRMDHEFVAKGHLFRKGIMKIMVYKIFRILVPGNTDSTEALSLSYLVELSVVAPAGQDMVSDDMKNFAEQLKPLVHLEKIDPKRLM

Target ORF information:

RefSeq Version	NM_017638.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 18 (MED18), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017638.3

ORF Insert Sequence:

ATGGAGGCAC CTCCAGTCAC CATGATGCCT GTCACTGGGG GCACCATTAA CATGATGGAG 
TACCTGTTGC AGGGAAGTGT TTTAGATCAC AGTTTGGAAA GCCTCATCCA CCGCCTTCGT 
GGTTTGTGTG ACAACATGGA ACCTGAGACT TTCCTTGACC ATGAGATGGT ATTCCTCCTT 
AAGGGCCAGC AAGCCAGCCC ATTTGTTCTC AGGGCCCGAC GCTCTATGGA CAGGGCAGGG 
GCACCCTGGC ATCTGCGCTA CCTGGGACAG CCAGAAATGG GAGACAAGAA CCGCCATGCC 
CTGGTGCGAA ACTGCGTGGA CATTGCCACA TCTGAGAACC TCACCGACTT CTTGATGGAA 
ATGGGCTTCC GCATGGACCA TGAGTTTGTT GCTAAGGGAC ATTTGTTCCG TAAGGGCATC 
ATGAAGATTA TGGTGTACAA GATTTTCCGC ATCCTGGTGC CAGGGAACAC AGACAGCACT 
GAGGCCTTGT CACTCTCCTA TCTCGTGGAA TTAAGTGTGG TAGCACCCGC TGGGCAGGAC 
ATGGTCTCTG ATGACATGAA GAACTTCGCA GAACAGCTAA AACCTCTGGT TCACCTAGAG 
AAAATAGACC CCAAGAGGCT CATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED18 ( NM_017638.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_060108.2 Homo sapiens mediator complex subunit 18 (MED18), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu24315
Clone ID Related Accession (Same CDS sequence)	NM_001127350.1 , XM_005245914.3 , NM_017638.3 , NM_017638.2 , XM_005245914.4 , NM_001127350.2
Accession Version	NM_017638.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 18
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA090416.1, AK000052.1 and AL353622.33. On or before Aug 3, 2011 this sequence version replaced XM_003403718.1, NM_017638.1. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.108373.1, SRR1803613.139732.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCAC CTCCAGTCAC CATGATGCCT GTCACTGGGG GCACCATTAA CATGATGGAG 
TACCTGTTGC AGGGAAGTGT TTTAGATCAC AGTTTGGAAA GCCTCATCCA CCGCCTTCGT 
GGTTTGTGTG ACAACATGGA ACCTGAGACT TTCCTTGACC ATGAGATGGT ATTCCTCCTT 
AAGGGCCAGC AAGCCAGCCC ATTTGTTCTC AGGGCCCGAC GCTCTATGGA CAGGGCAGGG 
GCACCCTGGC ATCTGCGCTA CCTGGGACAG CCAGAAATGG GAGACAAGAA CCGCCATGCC 
CTGGTGCGAA ACTGCGTGGA CATTGCCACA TCTGAGAACC TCACCGACTT CTTGATGGAA 
ATGGGCTTCC GCATGGACCA TGAGTTTGTT GCTAAGGGAC ATTTGTTCCG TAAGGGCATC 
ATGAAGATTA TGGTGTACAA GATTTTCCGC ATCCTGGTGC CAGGGAACAC AGACAGCACT 
GAGGCCTTGT CACTCTCCTA TCTCGTGGAA TTAAGTGTGG TAGCACCCGC TGGGCAGGAC 
ATGGTCTCTG ATGACATGAA GAACTTCGCA GAACAGCTAA AACCTCTGGT TCACCTAGAG 
AAAATAGACC CCAAGAGGCT CATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060108.2
CDS	246..872
Translation	MEAPPVTMMPVTGGTINMMEYLLQGSVLDHSLESLIHRLRGLCDNMEPETFLDHEMVFLLKGQQASPFVLRARRSMDRAGAPWHLRYLGQPEMGDKNRHALVRNCVDIATSENLTDFLMEMGFRMDHEFVAKGHLFRKGIMKIMVYKIFRILVPGNTDSTEALSLSYLVELSVVAPAGQDMVSDDMKNFAEQLKPLVHLEKIDPKRLM

Target ORF information:

RefSeq Version	NM_017638.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 18 (MED18), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017638.2

ORF Insert Sequence:

ATGGAGGCAC CTCCAGTCAC CATGATGCCT GTCACTGGGG GCACCATTAA CATGATGGAG 
TACCTGTTGC AGGGAAGTGT TTTAGATCAC AGTTTGGAAA GCCTCATCCA CCGCCTTCGT 
GGTTTGTGTG ACAACATGGA ACCTGAGACT TTCCTTGACC ATGAGATGGT ATTCCTCCTT 
AAGGGCCAGC AAGCCAGCCC ATTTGTTCTC AGGGCCCGAC GCTCTATGGA CAGGGCAGGG 
GCACCCTGGC ATCTGCGCTA CCTGGGACAG CCAGAAATGG GAGACAAGAA CCGCCATGCC 
CTGGTGCGAA ACTGCGTGGA CATTGCCACA TCTGAGAACC TCACCGACTT CTTGATGGAA 
ATGGGCTTCC GCATGGACCA TGAGTTTGTT GCTAAGGGAC ATTTGTTCCG TAAGGGCATC 
ATGAAGATTA TGGTGTACAA GATTTTCCGC ATCCTGGTGC CAGGGAACAC AGACAGCACT 
GAGGCCTTGT CACTCTCCTA TCTCGTGGAA TTAAGTGTGG TAGCACCCGC TGGGCAGGAC 
ATGGTCTCTG ATGACATGAA GAACTTCGCA GAACAGCTAA AACCTCTGGT TCACCTAGAG 
AAAATAGACC CCAAGAGGCT CATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED18 ( NM_001127350.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001120822.1 Homo sapiens mediator complex subunit 18 (MED18), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu24315
Clone ID Related Accession (Same CDS sequence)	NM_001127350.1 , XM_005245914.3 , NM_017638.3 , NM_017638.2 , XM_005245914.4 , NM_001127350.2
Accession Version	NM_001127350.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570550400000
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 18
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG752399.1 and AL353622.33. On May 31, 2019 this sequence version replaced NM_001127350.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.129010.1, DRR138512.679939.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCAC CTCCAGTCAC CATGATGCCT GTCACTGGGG GCACCATTAA CATGATGGAG 
TACCTGTTGC AGGGAAGTGT TTTAGATCAC AGTTTGGAAA GCCTCATCCA CCGCCTTCGT 
GGTTTGTGTG ACAACATGGA ACCTGAGACT TTCCTTGACC ATGAGATGGT ATTCCTCCTT 
AAGGGCCAGC AAGCCAGCCC ATTTGTTCTC AGGGCCCGAC GCTCTATGGA CAGGGCAGGG 
GCACCCTGGC ATCTGCGCTA CCTGGGACAG CCAGAAATGG GAGACAAGAA CCGCCATGCC 
CTGGTGCGAA ACTGCGTGGA CATTGCCACA TCTGAGAACC TCACCGACTT CTTGATGGAA 
ATGGGCTTCC GCATGGACCA TGAGTTTGTT GCTAAGGGAC ATTTGTTCCG TAAGGGCATC 
ATGAAGATTA TGGTGTACAA GATTTTCCGC ATCCTGGTGC CAGGGAACAC AGACAGCACT 
GAGGCCTTGT CACTCTCCTA TCTCGTGGAA TTAAGTGTGG TAGCACCCGC TGGGCAGGAC 
ATGGTCTCTG ATGACATGAA GAACTTCGCA GAACAGCTAA AACCTCTGGT TCACCTAGAG 
AAAATAGACC CCAAGAGGCT CATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120822.1
CDS	152..778
Translation	MEAPPVTMMPVTGGTINMMEYLLQGSVLDHSLESLIHRLRGLCDNMEPETFLDHEMVFLLKGQQASPFVLRARRSMDRAGAPWHLRYLGQPEMGDKNRHALVRNCVDIATSENLTDFLMEMGFRMDHEFVAKGHLFRKGIMKIMVYKIFRILVPGNTDSTEALSLSYLVELSVVAPAGQDMVSDDMKNFAEQLKPLVHLEKIDPKRLM

Target ORF information:

RefSeq Version	NM_001127350.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 18 (MED18), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127350.2

ORF Insert Sequence:

ATGGAGGCAC CTCCAGTCAC CATGATGCCT GTCACTGGGG GCACCATTAA CATGATGGAG 
TACCTGTTGC AGGGAAGTGT TTTAGATCAC AGTTTGGAAA GCCTCATCCA CCGCCTTCGT 
GGTTTGTGTG ACAACATGGA ACCTGAGACT TTCCTTGACC ATGAGATGGT ATTCCTCCTT 
AAGGGCCAGC AAGCCAGCCC ATTTGTTCTC AGGGCCCGAC GCTCTATGGA CAGGGCAGGG 
GCACCCTGGC ATCTGCGCTA CCTGGGACAG CCAGAAATGG GAGACAAGAA CCGCCATGCC 
CTGGTGCGAA ACTGCGTGGA CATTGCCACA TCTGAGAACC TCACCGACTT CTTGATGGAA 
ATGGGCTTCC GCATGGACCA TGAGTTTGTT GCTAAGGGAC ATTTGTTCCG TAAGGGCATC 
ATGAAGATTA TGGTGTACAA GATTTTCCGC ATCCTGGTGC CAGGGAACAC AGACAGCACT 
GAGGCCTTGT CACTCTCCTA TCTCGTGGAA TTAAGTGTGG TAGCACCCGC TGGGCAGGAC 
ATGGTCTCTG ATGACATGAA GAACTTCGCA GAACAGCTAA AACCTCTGGT TCACCTAGAG 
AAAATAGACC CCAAGAGGCT CATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED18 ( XM_005245914.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245971.1 Homo sapiens mediator complex subunit 18 (MED18), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu24315
Clone ID Related Accession (Same CDS sequence)	NM_001127350.1 , XM_005245914.3 , NM_017638.3 , NM_017638.2 , XM_005245914.4 , NM_001127350.2
Accession Version	XM_005245914.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426003200000
Organism	Homo sapiens(Human)
Product	mediator of RNA polymerase II transcription subunit 18 isoform X1
Comment

ATGGAGGCAC CTCCAGTCAC CATGATGCCT GTCACTGGGG GCACCATTAA CATGATGGAG 
TACCTGTTGC AGGGAAGTGT TTTAGATCAC AGTTTGGAAA GCCTCATCCA CCGCCTTCGT 
GGTTTGTGTG ACAACATGGA ACCTGAGACT TTCCTTGACC ATGAGATGGT ATTCCTCCTT 
AAGGGCCAGC AAGCCAGCCC ATTTGTTCTC AGGGCCCGAC GCTCTATGGA CAGGGCAGGG 
GCACCCTGGC ATCTGCGCTA CCTGGGACAG CCAGAAATGG GAGACAAGAA CCGCCATGCC 
CTGGTGCGAA ACTGCGTGGA CATTGCCACA TCTGAGAACC TCACCGACTT CTTGATGGAA 
ATGGGCTTCC GCATGGACCA TGAGTTTGTT GCTAAGGGAC ATTTGTTCCG TAAGGGCATC 
ATGAAGATTA TGGTGTACAA GATTTTCCGC ATCCTGGTGC CAGGGAACAC AGACAGCACT 
GAGGCCTTGT CACTCTCCTA TCTCGTGGAA TTAAGTGTGG TAGCACCCGC TGGGCAGGAC 
ATGGTCTCTG ATGACATGAA GAACTTCGCA GAACAGCTAA AACCTCTGGT TCACCTAGAG 
AAAATAGACC CCAAGAGGCT CATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245971.1
Translation	MEAPPVTMMPVTGGTINMMEYLLQGSVLDHSLESLIHRLRGLCDNMEPETFLDHEMVFLLKGQQASPFVLRARRSMDRAGAPWHLRYLGQPEMGDKNRHALVRNCVDIATSENLTDFLMEMGFRMDHEFVAKGHLFRKGIMKIMVYKIFRILVPGNTDSTEALSLSYLVELSVVAPAGQDMVSDDMKNFAEQLKPLVHLEKIDPKRLM

Target ORF information:

RefSeq Version	XM_005245914.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens mediator complex subunit 18 (MED18), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005245914.3

ORF Insert Sequence:

ATGGAGGCAC CTCCAGTCAC CATGATGCCT GTCACTGGGG GCACCATTAA CATGATGGAG 
TACCTGTTGC AGGGAAGTGT TTTAGATCAC AGTTTGGAAA GCCTCATCCA CCGCCTTCGT 
GGTTTGTGTG ACAACATGGA ACCTGAGACT TTCCTTGACC ATGAGATGGT ATTCCTCCTT 
AAGGGCCAGC AAGCCAGCCC ATTTGTTCTC AGGGCCCGAC GCTCTATGGA CAGGGCAGGG 
GCACCCTGGC ATCTGCGCTA CCTGGGACAG CCAGAAATGG GAGACAAGAA CCGCCATGCC 
CTGGTGCGAA ACTGCGTGGA CATTGCCACA TCTGAGAACC TCACCGACTT CTTGATGGAA 
ATGGGCTTCC GCATGGACCA TGAGTTTGTT GCTAAGGGAC ATTTGTTCCG TAAGGGCATC 
ATGAAGATTA TGGTGTACAA GATTTTCCGC ATCCTGGTGC CAGGGAACAC AGACAGCACT 
GAGGCCTTGT CACTCTCCTA TCTCGTGGAA TTAAGTGTGG TAGCACCCGC TGGGCAGGAC 
ATGGTCTCTG ATGACATGAA GAACTTCGCA GAACAGCTAA AACCTCTGGT TCACCTAGAG 
AAAATAGACC CCAAGAGGCT CATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED18 ( XM_005245914.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245971.1 Homo sapiens mediator complex subunit 18 (MED18), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu24315
Clone ID Related Accession (Same CDS sequence)	NM_001127350.1 , XM_005245914.3 , NM_017638.3 , NM_017638.2 , XM_005245914.4 , NM_001127350.2
Accession Version	XM_005245914.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 18 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005245914.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAC CTCCAGTCAC CATGATGCCT GTCACTGGGG GCACCATTAA CATGATGGAG 
TACCTGTTGC AGGGAAGTGT TTTAGATCAC AGTTTGGAAA GCCTCATCCA CCGCCTTCGT 
GGTTTGTGTG ACAACATGGA ACCTGAGACT TTCCTTGACC ATGAGATGGT ATTCCTCCTT 
AAGGGCCAGC AAGCCAGCCC ATTTGTTCTC AGGGCCCGAC GCTCTATGGA CAGGGCAGGG 
GCACCCTGGC ATCTGCGCTA CCTGGGACAG CCAGAAATGG GAGACAAGAA CCGCCATGCC 
CTGGTGCGAA ACTGCGTGGA CATTGCCACA TCTGAGAACC TCACCGACTT CTTGATGGAA 
ATGGGCTTCC GCATGGACCA TGAGTTTGTT GCTAAGGGAC ATTTGTTCCG TAAGGGCATC 
ATGAAGATTA TGGTGTACAA GATTTTCCGC ATCCTGGTGC CAGGGAACAC AGACAGCACT 
GAGGCCTTGT CACTCTCCTA TCTCGTGGAA TTAAGTGTGG TAGCACCCGC TGGGCAGGAC 
ATGGTCTCTG ATGACATGAA GAACTTCGCA GAACAGCTAA AACCTCTGGT TCACCTAGAG 
AAAATAGACC CCAAGAGGCT CATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245971.1
CDS	160..786
Translation	MEAPPVTMMPVTGGTINMMEYLLQGSVLDHSLESLIHRLRGLCDNMEPETFLDHEMVFLLKGQQASPFVLRARRSMDRAGAPWHLRYLGQPEMGDKNRHALVRNCVDIATSENLTDFLMEMGFRMDHEFVAKGHLFRKGIMKIMVYKIFRILVPGNTDSTEALSLSYLVELSVVAPAGQDMVSDDMKNFAEQLKPLVHLEKIDPKRLM

Target ORF information:

RefSeq Version	XM_005245914.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 18 (MED18), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005245914.4

ORF Insert Sequence:

ATGGAGGCAC CTCCAGTCAC CATGATGCCT GTCACTGGGG GCACCATTAA CATGATGGAG 
TACCTGTTGC AGGGAAGTGT TTTAGATCAC AGTTTGGAAA GCCTCATCCA CCGCCTTCGT 
GGTTTGTGTG ACAACATGGA ACCTGAGACT TTCCTTGACC ATGAGATGGT ATTCCTCCTT 
AAGGGCCAGC AAGCCAGCCC ATTTGTTCTC AGGGCCCGAC GCTCTATGGA CAGGGCAGGG 
GCACCCTGGC ATCTGCGCTA CCTGGGACAG CCAGAAATGG GAGACAAGAA CCGCCATGCC 
CTGGTGCGAA ACTGCGTGGA CATTGCCACA TCTGAGAACC TCACCGACTT CTTGATGGAA 
ATGGGCTTCC GCATGGACCA TGAGTTTGTT GCTAAGGGAC ATTTGTTCCG TAAGGGCATC 
ATGAAGATTA TGGTGTACAA GATTTTCCGC ATCCTGGTGC CAGGGAACAC AGACAGCACT 
GAGGCCTTGT CACTCTCCTA TCTCGTGGAA TTAAGTGTGG TAGCACCCGC TGGGCAGGAC 
ATGGTCTCTG ATGACATGAA GAACTTCGCA GAACAGCTAA AACCTCTGGT TCACCTAGAG 
AAAATAGACC CCAAGAGGCT CATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Mediator MED18 subunit plays a negative role in transcription via the CDK/cyclin module.
Genes to cells : devoted to molecular & cellular mechanisms19(7)582-93(2014 Jul)
Kumafuji M,Umemura H,Furumoto T,Fukasawa R,Tanaka A,Ohkuma Y

Subunit architecture and functional modular rearrangements of the transcriptional mediator complex.
Cell157(6)1430-44(2014 Jun)
Tsai KL,Tomomori-Sato C,Sato S,Conaway RC,Conaway JW,Asturias FJ

A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology.
Molecular cell14(5)685-91(2004 Jun)
Sato S,Tomomori-Sato C,Parmely TJ,Florens L,Zybailov B,Swanson SK,Banks CA,Jin J,Cai Y,Washburn MP,Conaway JW,Conaway RC

A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2.
The Journal of biological chemistry279(7)5846-51(2004 Feb)
Tomomori-Sato C,Sato S,Parmely TJ,Banks CA,Sorokina I,Florens L,Zybailov B,Washburn MP,Brower CS,Conaway RC,Conaway JW

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

MED18 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM