POLE3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLE3
Entrez Gene ID	54107
Full Name	DNA polymerase epsilon 3, accessory subunit
Synonyms	CHARAC17,CHRAC17,YBL1,p17
General protein information	Preferred Names DNA polymerase epsilon 3, accessory subunit Names DNA polymerase epsilon subunit 3 CHRAC-17 DNA polymerase II subunit 3 DNA polymerase epsilon p17 subunit DNA polymerase epsilon subunit p17 arsenic transactivated protein asTP chromatin accessibility complex 17 kDa protein histone fold protein CHRAC17 huCHRAC17 polymerase (DNA directed), epsilon 3 (p17 subunit) polymerase (DNA directed), epsilon 3, accessory subunit polymerase (DNA) epsilon 3, accessory subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q32
Summary	POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004].
Disorder MIM:	607267

mRNA and Protein(s)

mRNA	Protein	Name
NM_001278255.1	NP_001265184.1	DNA polymerase epsilon subunit 3
NM_017443.4	NP_059139.3	DNA polymerase epsilon subunit 3
NM_017443.5	NP_059139.3	DNA polymerase epsilon subunit 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	POLE3	NP_059139.3
Drosophila melanogaster (fruit fly)	Chrac-14	NP_476646.2
Macaca mulatta (Rhesus monkey)	POLE3	XP_001101300.1
Canis lupus familiaris (dog)	POLE3	XP_855374.2
Gallus gallus (chicken)	POLE3	NP_001020525.2
Danio rerio (zebrafish)	pole3	NP_957095.1
Bos taurus (cattle)	POLE3	NP_001029562.1
Rattus norvegicus (Norway rat)	Pole3	NP_001007653.1
Rattus norvegicus (Norway rat)	LOC100362333	XP_002729875.1
Pan troglodytes (chimpanzee)	POLE3	NP_001233445.1
Mus musculus (house mouse)	Pole3	NP_067473.2
Xenopus tropicalis (tropical clawed frog)	pole3	NP_001017264.1

Gene Ontology
Bibliography

Related articles in PubMed

POLE3-POLE4 Is a Histone H3-H4 Chaperone that Maintains Chromatin Integrity during DNA Replication.
Bellelli R, Belan O, Pye VE, Clement C, Maslen SL, Skehel JM, Cherepanov P, Almouzni G, Boulton SJ
Molecular cell72(1)112-126.e5(2018 10)

Orthoxenografts of Testicular Germ Cell Tumors Demonstrate Genomic Changes Associated with Cisplatin Resistance and Identify PDMP as a Resensitizing Agent.
Piulats JM, Vidal A, García-Rodríguez FJ, Muñoz C, Nadal M, Moutinho C, Martínez-Iniesta M, Mora J, Figueras A, Guinó E, Padullés L, Aytés À, Molleví DG, Puertas S, Martínez-Fernández C, Castillo W, Juliachs M, Moreno V, Muñoz P, Stefanovic M, Pujana MA, Condom E, Esteller M, Germà JR, Capella G, Farré L, Morales A, Viñals F, García-Del-Muro X, Cerón J, Villanueva A
Clinical cancer research : an official journal of the American Association for Cancer Research24(15)3755-3766(2018 08)

Global landscape of HIV-human protein complexes.
Jäger S, Cimermancic P, Gulbahce N, Johnson JR, McGovern KE, Clarke SC, Shales M, Mercenne G, Pache L, Li K, Hernandez H, Jang GM, Roth SL, Akiva E, Marlett J, Stephens M, D'Orso I, Fernandes J, Fahey M, Mahon C, O'Donoghue AJ, Todorovic A, Morris JH, Maltby DA, Alber T, Cagney G, Bushman FD, Young JA, Chanda SK, Sundquist WI, Kortemme T, Hernandez RD, Craik CS, Burlingame A, Sali A, Frankel AD, Krogan NJ
Nature481(7381)365-70(2011 Dec)

Next-generation sequencing to generate interactome datasets.
Yu H, Tardivo L, Tam S, Weiner E, Gebreab F, Fan C, Svrzikapa N, Hirozane-Kishikawa T, Rietman E, Yang X, Sahalie J, Salehi-Ashtiani K, Hao T, Cusick ME, Hill DE, Roth FP, Braun P, Vidal M
Nature methods8(6)478-80(2011 Jun)

Human ATAC Is a GCN5/PCAF-containing acetylase complex with a novel NC2-like histone fold module that interacts with the TATA-binding protein.
Wang YL, Faiola F, Xu M, Pan S, Martinez E
The Journal of biological chemistry283(49)33808-15(2008 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POLE3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLE3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02349	NM_017443.5 Latest version!	Homo sapiens DNA polymerase epsilon 3, accessory subunit (POLE3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu02349	NM_001278255.1 Latest version!	Homo sapiens DNA polymerase epsilon 3, accessory subunit (POLE3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
View Old Accession Versions >>
OHu02349	NM_017443.4	Homo sapiens DNA polymerase epsilon 3, accessory subunit (POLE3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLE3 ( NM_017443.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_059139.3 Homo sapiens DNA polymerase epsilon 3, accessory subunit (POLE3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02349
Clone ID Related Accession (Same CDS sequence)	NM_001278255.1 , NM_017443.4 , NM_017443.5
Accession Version	NM_017443.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Homo sapiens(human)
Product	DNA polymerase epsilon subunit 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA908447.1, AK074762.1 and AF070640.1. On Mar 6, 2009 this sequence version replaced NM_017443.3. Transcript Variant: This variant (1) represents the longer protein-coding transcript. Variants 1 and 2 both encode the same protein. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.96638.1, AK074762.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGA GGCCCGAGGA CCTAAACCTG CCCAATGCCG TGATCACCAG GATCATCAAG 
GAGGCGCTCC CGGACGGTGT CAACATCTCC AAGGAGGCCC GGAGCGCCAT CTCCCGCGCC 
GCCAGCGTCT TCGTGCTGTA CGCCACATCC TGTGCTAACA ACTTTGCAAT GAAAGGAAAG 
CGGAAGACGC TGAATGCCAG TGATGTGCTC TCAGCCATGG AAGAGATGGA GTTCCAGCGG 
TTCGTTACCC CATTGAAAGA AGCTCTGGAA GCATATAGGC GGGAGCAGAA AGGCAAGAAG 
GAGGCCTCAG AGCAAAAGAA GAAGGACAAA GACAAAAAAA CAGACTCGGA AGAGCAAGAC 
AAGAGCAGGG ATGAGGACAA TGATGAAGAC GAAGAAAGGC TGGAAGAAGA AGAACAGAAT 
GAAGAGGAAG AAGTAGACAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_059139.3
CDS	249..692
Translation	MAERPEDLNLPNAVITRIIKEALPDGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTLNASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKKTDSEEQDKSRDEDNDEDEERLEEEEQNEEEEVDN

Target ORF information:

RefSeq Version	NM_017443.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase epsilon 3, accessory subunit (POLE3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017443.4

ORF Insert Sequence:

ATGGCGGAGA GGCCCGAGGA CCTAAACCTG CCCAATGCCG TGATCACCAG GATCATCAAG 
GAGGCGCTCC CGGACGGTGT CAACATCTCC AAGGAGGCCC GGAGCGCCAT CTCCCGCGCC 
GCCAGCGTCT TCGTGCTGTA CGCCACATCC TGTGCTAACA ACTTTGCAAT GAAAGGAAAG 
CGGAAGACGC TGAATGCCAG TGATGTGCTC TCAGCCATGG AAGAGATGGA GTTCCAGCGG 
TTCGTTACCC CATTGAAAGA AGCTCTGGAA GCATATAGGC GGGAGCAGAA AGGCAAGAAG 
GAGGCCTCAG AGCAAAAGAA GAAGGACAAA GACAAAAAAA CAGACTCGGA AGAGCAAGAC 
AAGAGCAGGG ATGAGGACAA TGATGAAGAC GAAGAAAGGC TGGAAGAAGA AGAACAGAAT 
GAAGAGGAAG AAGTAGACAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLE3 ( NM_001278255.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265184.1 Homo sapiens DNA polymerase epsilon 3, accessory subunit (POLE3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02349
Clone ID Related Accession (Same CDS sequence)	NM_001278255.1 , NM_017443.4 , NM_017443.5
Accession Version	NM_001278255.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Homo sapiens(human)
Product	DNA polymerase epsilon subunit 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA908447.1, DA477404.1, AK074762.1 and AF070640.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. ##Evidence-Data-START## Transcript exon combination :: BM552527.1, DA477404.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGA GGCCCGAGGA CCTAAACCTG CCCAATGCCG TGATCACCAG GATCATCAAG 
GAGGCGCTCC CGGACGGTGT CAACATCTCC AAGGAGGCCC GGAGCGCCAT CTCCCGCGCC 
GCCAGCGTCT TCGTGCTGTA CGCCACATCC TGTGCTAACA ACTTTGCAAT GAAAGGAAAG 
CGGAAGACGC TGAATGCCAG TGATGTGCTC TCAGCCATGG AAGAGATGGA GTTCCAGCGG 
TTCGTTACCC CATTGAAAGA AGCTCTGGAA GCATATAGGC GGGAGCAGAA AGGCAAGAAG 
GAGGCCTCAG AGCAAAAGAA GAAGGACAAA GACAAAAAAA CAGACTCGGA AGAGCAAGAC 
AAGAGCAGGG ATGAGGACAA TGATGAAGAC GAAGAAAGGC TGGAAGAAGA AGAACAGAAT 
GAAGAGGAAG AAGTAGACAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265184.1
CDS	219..662
Translation	MAERPEDLNLPNAVITRIIKEALPDGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTLNASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKKTDSEEQDKSRDEDNDEDEERLEEEEQNEEEEVDN

Target ORF information:

RefSeq Version	NM_001278255.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase epsilon 3, accessory subunit (POLE3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278255.1

ORF Insert Sequence:

ATGGCGGAGA GGCCCGAGGA CCTAAACCTG CCCAATGCCG TGATCACCAG GATCATCAAG 
GAGGCGCTCC CGGACGGTGT CAACATCTCC AAGGAGGCCC GGAGCGCCAT CTCCCGCGCC 
GCCAGCGTCT TCGTGCTGTA CGCCACATCC TGTGCTAACA ACTTTGCAAT GAAAGGAAAG 
CGGAAGACGC TGAATGCCAG TGATGTGCTC TCAGCCATGG AAGAGATGGA GTTCCAGCGG 
TTCGTTACCC CATTGAAAGA AGCTCTGGAA GCATATAGGC GGGAGCAGAA AGGCAAGAAG 
GAGGCCTCAG AGCAAAAGAA GAAGGACAAA GACAAAAAAA CAGACTCGGA AGAGCAAGAC 
AAGAGCAGGG ATGAGGACAA TGATGAAGAC GAAGAAAGGC TGGAAGAAGA AGAACAGAAT 
GAAGAGGAAG AAGTAGACAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLE3 ( NM_017443.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_059139.3 Homo sapiens DNA polymerase epsilon 3, accessory subunit (POLE3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02349
Clone ID Related Accession (Same CDS sequence)	NM_001278255.1 , NM_017443.4 , NM_017443.5
Accession Version	NM_017443.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Homo sapiens(human)
Product	DNA polymerase epsilon subunit 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA908447.1, AK074762.1 and AF070640.1. On Nov 22, 2018 this sequence version replaced NM_017443.4. Transcript Variant: This variant (1) represents the longer protein-coding transcript. Variants 1 and 2 both encode the same protein. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.96638.1, AK074762.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000374171.5/ ENSP00000363286.4 RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGAGA GGCCCGAGGA CCTAAACCTG CCCAATGCCG TGATCACCAG GATCATCAAG 
GAGGCGCTCC CGGACGGTGT CAACATCTCC AAGGAGGCCC GGAGCGCCAT CTCCCGCGCC 
GCCAGCGTCT TCGTGCTGTA CGCCACATCC TGTGCTAACA ACTTTGCAAT GAAAGGAAAG 
CGGAAGACGC TGAATGCCAG TGATGTGCTC TCAGCCATGG AAGAGATGGA GTTCCAGCGG 
TTCGTTACCC CATTGAAAGA AGCTCTGGAA GCATATAGGC GGGAGCAGAA AGGCAAGAAG 
GAGGCCTCAG AGCAAAAGAA GAAGGACAAA GACAAAAAAA CAGACTCGGA AGAGCAAGAC 
AAGAGCAGGG ATGAGGACAA TGATGAAGAC GAAGAAAGGC TGGAAGAAGA AGAACAGAAT 
GAAGAGGAAG AAGTAGACAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_059139.3
CDS	175..618
Translation	MAERPEDLNLPNAVITRIIKEALPDGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTLNASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKKTDSEEQDKSRDEDNDEDEERLEEEEQNEEEEVDN

Target ORF information:

RefSeq Version	NM_017443.5
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase epsilon 3, accessory subunit (POLE3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017443.5

ORF Insert Sequence:

ATGGCGGAGA GGCCCGAGGA CCTAAACCTG CCCAATGCCG TGATCACCAG GATCATCAAG 
GAGGCGCTCC CGGACGGTGT CAACATCTCC AAGGAGGCCC GGAGCGCCAT CTCCCGCGCC 
GCCAGCGTCT TCGTGCTGTA CGCCACATCC TGTGCTAACA ACTTTGCAAT GAAAGGAAAG 
CGGAAGACGC TGAATGCCAG TGATGTGCTC TCAGCCATGG AAGAGATGGA GTTCCAGCGG 
TTCGTTACCC CATTGAAAGA AGCTCTGGAA GCATATAGGC GGGAGCAGAA AGGCAAGAAG 
GAGGCCTCAG AGCAAAAGAA GAAGGACAAA GACAAAAAAA CAGACTCGGA AGAGCAAGAC 
AAGAGCAGGG ATGAGGACAA TGATGAAGAC GAAGAAAGGC TGGAAGAAGA AGAACAGAAT 
GAAGAGGAAG AAGTAGACAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

POLE3-POLE4 Is a Histone H3-H4 Chaperone that Maintains Chromatin Integrity during DNA Replication.
Molecular cell72(1)112-126.e5(2018 10)
Bellelli R,Belan O,Pye VE,Clement C,Maslen SL,Skehel JM,Cherepanov P,Almouzni G,Boulton SJ

Orthoxenografts of Testicular Germ Cell Tumors Demonstrate Genomic Changes Associated with Cisplatin Resistance and Identify PDMP as a Resensitizing Agent.
Clinical cancer research : an official journal of the American Association for Cancer Research24(15)3755-3766(2018 08)
Piulats JM,Vidal A,García-Rodríguez FJ,Muñoz C,Nadal M,Moutinho C,Martínez-Iniesta M,Mora J,Figueras A,Guinó E,Padullés L,Aytés À,Molleví DG,Puertas S,Martínez-Fernández C,Castillo W,Juliachs M,Moreno V,Muñoz P,Stefanovic M,Pujana MA,Condom E,Esteller M,Germà JR,Capella G,Farré L,Morales A,Viñals F,García-Del-Muro X,Cerón J,Villanueva A

Global landscape of HIV-human protein complexes.
Nature481(7381)365-70(2011 Dec)
Jäger S,Cimermancic P,Gulbahce N,Johnson JR,McGovern KE,Clarke SC,Shales M,Mercenne G,Pache L,Li K,Hernandez H,Jang GM,Roth SL,Akiva E,Marlett J,Stephens M,D'Orso I,Fernandes J,Fahey M,Mahon C,O'Donoghue AJ,Todorovic A,Morris JH,Maltby DA,Alber T,Cagney G,Bushman FD,Young JA,Chanda SK,Sundquist WI,Kortemme T,Hernandez RD,Craik CS,Burlingame A,Sali A,Frankel AD,Krogan NJ

Next-generation sequencing to generate interactome datasets.
Nature methods8(6)478-80(2011 Jun)
Yu H,Tardivo L,Tam S,Weiner E,Gebreab F,Fan C,Svrzikapa N,Hirozane-Kishikawa T,Rietman E,Yang X,Sahalie J,Salehi-Ashtiani K,Hao T,Cusick ME,Hill DE,Roth FP,Braun P,Vidal M

Human ATAC Is a GCN5/PCAF-containing acetylase complex with a novel NC2-like histone fold module that interacts with the TATA-binding protein.
The Journal of biological chemistry283(49)33808-15(2008 Dec)
Wang YL,Faiola F,Xu M,Pan S,Martinez E

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POLE3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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