MIS18A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MIS18A
Entrez Gene ID	54069
Full Name	MIS18 kinetochore protein A
Synonyms	B28,C21orf45,C21orf46,FASP1,MIS18alpha,hMis18alpha
General protein information	Preferred Names MIS18 kinetochore protein A Names protein Mis18-alpha FAPP1-associated protein 1 MIS18 kinetochore protein homolog A
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 21 Map Location: 21q22.11

mRNA and Protein(s)

mRNA	Protein	Name
NM_018944.2	NP_061817.1	protein Mis18-alpha
XM_017028401.1	XP_016883890.1	protein Mis18-alpha isoform X2
XM_017028400.1	XP_016883889.1	protein Mis18-alpha isoform X1
XM_017028402.1	XP_016883891.1	protein Mis18-alpha isoform X3
NM_018944.3	NP_061817.1	protein Mis18-alpha

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mis18a	NP_001120995.2
Xenopus tropicalis (tropical clawed frog)	mis18a	XP_002938488.2
Homo sapiens (human)	MIS18A	NP_061817.1
Canis lupus familiaris (dog)	MIS18A	XP_852107.1
Bos taurus (cattle)	MIS18A	NP_001091479.1
Mus musculus (house mouse)	Mis18a	NP_079918.1
Danio rerio (zebrafish)	mis18a	XP_002663634.2
Macaca mulatta (Rhesus monkey)	LOC709275	XP_001097780.2
Pan troglodytes (chimpanzee)	MIS18A	NP_001065279.1
Gallus gallus (chicken)	MIS18A	XP_416702.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Skin-Specific Deletion of Mis18α Impedes Proliferation and Stratification of Epidermal Keratinocytes.
Park KC, Lee M, Jeon Y, Jeon R, Baek SH, Lee H, Kim KI
The Journal of investigative dermatology137(2)414-421(2017 02)

CDK-regulated dimerization of M18BP1 on a Mis18 hexamer is necessary for CENP-A loading.
Pan D, Klare K, Petrovic A, Take A, Walstein K, Singh P, Rondelet A, Bird AW, Musacchio A
eLife6(2017 01)

Differential Binding Partners of the Mis18α/β YIPPEE Domains Regulate Mis18 Complex Recruitment to Centromeres.
Stellfox ME, Nardi IK, Knippler CM, Foltz DR
Cell reports15(10)2127-2135(2016 06)

Licensing of Centromeric Chromatin Assembly through the Mis18α-Mis18β Heterotetramer.
Nardi IK, Zasadzińska E, Stellfox ME, Knippler CM, Foltz DR
Molecular cell61(5)774-787(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MIS18A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MIS18A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu93276	XM_017028400.1 Latest version!	Homo sapiens MIS18 kinetochore protein A (MIS18A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu93277	XM_017028401.1 Latest version!	Homo sapiens MIS18 kinetochore protein A (MIS18A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu93278	XM_017028402.1 Latest version!	Homo sapiens MIS18 kinetochore protein A (MIS18A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu02346	NM_018944.3 Latest version!	Homo sapiens MIS18 kinetochore protein A (MIS18A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
View Old Accession Versions >>
OHu02346	NM_018944.2	Homo sapiens MIS18 kinetochore protein A (MIS18A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MIS18A ( NM_018944.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_061817.1 Homo sapiens MIS18 kinetochore protein A (MIS18A), mRNA.

Price & Availability↑

CloneID	OHu02346
Clone ID Related Accession (Same CDS sequence)	NM_018944.2 , NM_018944.3
Accession Version	NM_018944.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506700800000
Organism	Homo sapiens(human)
Product	protein Mis18-alpha
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB959520.1, BU616573.1, AK075281.1, BU567844.1, AW962893.1 and BU623181.1. On Mar 30, 2004 this sequence version replaced NM_018944.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.36739.1, SRR1163655.108931.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGGCG TTCGGTCACT GAGGTGTAGC AGAGGATGCG CTGGCGGCTG TGAGTGCGGC 
GACAAGGGCA AATGCAGCGA CTCCTCGCTG TTGGGCAAGA GACTCTCCGA AGACTCGAGC 
CGCCACCAGC TGTTGCAGAA GTGGGCGAGC ATGTGGAGCT CCATGAGCGA AGACGCGTCG 
GTGGCCGACA TGGAGAGGGC GCAGCTGGAG GAGGAGGCGG CGGCTGCGGA GGAGAGGCCG 
CTGGTGTTCC TGTGCTCCGG CTGCCGGCGG CCGCTGGGCG ACTCGCTGAG CTGGGTGGCC 
AGCCAGGAGG ACACCAACTG CATCCTGCTT CGCTGTGTTT CCTGTAATGT TTCTGTGGAT 
AAGGAACAGA AGCTATCCAA ACGTGAAAAG GAAAATGGTT GCGTCCTTGA GACTTTGTGC 
TGCGCGGGGT GCTCACTCAA TCTTGGCTAC GTGTACAGAT GCACGCCCAA GAATCTTGAT 
TACAAGAGAG ACTTGTTTTG CCTCAGTGTT GAAGCCATTG AAAGTTATGT TTTAGGGTCC 
TCTGAAAAGC AAATTGTGTC AGAAGATAAA GAGCTTTTTA ATCTTGAAAG CAGAGTTGAA 
ATAGAAAAGT CTCTAACACA GATGGAAGAT GTCTTGAAAG CATTACAAAT GAAGCTGTGG 
GAGGCCGAAT CCAAATTGTC CTTTGCCACT TGTAAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061817.1
CDS	52..753
Translation	MAGVRSLRCSRGCAGGCECGDKGKCSDSSLLGKRLSEDSSRHQLLQKWASMWSSMSEDASVADMERAQLEEEAAAAEERPLVFLCSGCRRPLGDSLSWVASQEDTNCILLRCVSCNVSVDKEQKLSKREKENGCVLETLCCAGCSLNLGYVYRCTPKNLDYKRDLFCLSVEAIESYVLGSSEKQIVSEDKELFNLESRVEIEKSLTQMEDVLKALQMKLWEAESKLSFATCKS

Target ORF information:

RefSeq Version	NM_018944.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MIS18 kinetochore protein A (MIS18A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018944.2

ORF Insert Sequence:

ATGGCAGGCG TTCGGTCACT GAGGTGTAGC AGAGGATGCG CTGGCGGCTG TGAGTGCGGC 
GACAAGGGCA AATGCAGCGA CTCCTCGCTG TTGGGCAAGA GACTCTCCGA AGACTCGAGC 
CGCCACCAGC TGTTGCAGAA GTGGGCGAGC ATGTGGAGCT CCATGAGCGA AGACGCGTCG 
GTGGCCGACA TGGAGAGGGC GCAGCTGGAG GAGGAGGCGG CGGCTGCGGA GGAGAGGCCG 
CTGGTGTTCC TGTGCTCCGG CTGCCGGCGG CCGCTGGGCG ACTCGCTGAG CTGGGTGGCC 
AGCCAGGAGG ACACCAACTG CATCCTGCTT CGCTGTGTTT CCTGTAATGT TTCTGTGGAT 
AAGGAACAGA AGCTATCCAA ACGTGAAAAG GAAAATGGTT GCGTCCTTGA GACTTTGTGC 
TGCGCGGGGT GCTCACTCAA TCTTGGCTAC GTGTACAGAT GCACGCCCAA GAATCTTGAT 
TACAAGAGAG ACTTGTTTTG CCTCAGTGTT GAAGCCATTG AAAGTTATGT TTTAGGGTCC 
TCTGAAAAGC AAATTGTGTC AGAAGATAAA GAGCTTTTTA ATCTTGAAAG CAGAGTTGAA 
ATAGAAAAGT CTCTAACACA GATGGAAGAT GTCTTGAAAG CATTACAAAT GAAGCTGTGG 
GAGGCCGAAT CCAAATTGTC CTTTGCCACT TGTAAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIS18A ( XM_017028400.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883889.1 Homo sapiens MIS18 kinetochore protein A (MIS18A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu93276
Clone ID Related Accession (Same CDS sequence)	XM_017028400.1
Accession Version	XM_017028400.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	protein Mis18-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGCG TTCGGTCACT GAGGTGTAGC AGAGGATGCG CTGGCGGCTG TGAGTGCGGC 
GACAAGGGCA AATGCAGCGA CTCCTCGCTG TTGGGCAAGA GACTCTCCGA AGACTCGAGC 
CGCCACCAGC TGTTGCAGAA GTGGGCGAGC ATGTGGAGCT CCATGAGCGA AGACGCGTCG 
GTGGCCGACA TGGAGAGGGC GCAGCTGGAG GAGGAGGCGG CGGCTGCGGA GGAGAGGCCG 
CTGGTGTTCC TGTGCTCCGG CTGCCGGCGG CCGCTGGGCG ACTCGCTGAG CTGGGTGGCC 
AGCCAGGAGG ACACCAACTG CATCCTGCTT CGCTGTGTTT CCTGTAATGT TTCTGTGGAT 
AAGGAACAGA AGCTATCCAA ACGTGAAAAG GAAAATGGTT GCGTCCTTGA GACTTTGTGC 
TGCGCGGGGT GCTCACTCAA TCTTGGCTAC GTGTACAGAT GCACGCCCAA GAATCTTGAT 
TACAAGAGAG ACTTGTTTTG CCTCAGTGTT GAAGCCATTG AAAGGGAGAT GATGCCTGGA 
GCTGTGAAAC AAATCCTGAG CCACGGGATG AGTGTAGACT TGAGACTCCA GAGTTTGGGA 
TCCTGGTCCA GATCATGCCA CTGCTTACCA AGTAGGGTCC TGCCTACCAT AATCCAGGAG 
GTGGTCTTCC AGACATTAAA TAGGATCATG AAATCAATGG CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883889.1
CDS	143..847
Translation	MAGVRSLRCSRGCAGGCECGDKGKCSDSSLLGKRLSEDSSRHQLLQKWASMWSSMSEDASVADMERAQLEEEAAAAEERPLVFLCSGCRRPLGDSLSWVASQEDTNCILLRCVSCNVSVDKEQKLSKREKENGCVLETLCCAGCSLNLGYVYRCTPKNLDYKRDLFCLSVEAIEREMMPGAVKQILSHGMSVDLRLQSLGSWSRSCHCLPSRVLPTIIQEVVFQTLNRIMKSMA

Target ORF information:

RefSeq Version	XM_017028400.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MIS18 kinetochore protein A (MIS18A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028400.1

ORF Insert Sequence:

ATGGCAGGCG TTCGGTCACT GAGGTGTAGC AGAGGATGCG CTGGCGGCTG TGAGTGCGGC 
GACAAGGGCA AATGCAGCGA CTCCTCGCTG TTGGGCAAGA GACTCTCCGA AGACTCGAGC 
CGCCACCAGC TGTTGCAGAA GTGGGCGAGC ATGTGGAGCT CCATGAGCGA AGACGCGTCG 
GTGGCCGACA TGGAGAGGGC GCAGCTGGAG GAGGAGGCGG CGGCTGCGGA GGAGAGGCCG 
CTGGTGTTCC TGTGCTCCGG CTGCCGGCGG CCGCTGGGCG ACTCGCTGAG CTGGGTGGCC 
AGCCAGGAGG ACACCAACTG CATCCTGCTT CGCTGTGTTT CCTGTAATGT TTCTGTGGAT 
AAGGAACAGA AGCTATCCAA ACGTGAAAAG GAAAATGGTT GCGTCCTTGA GACTTTGTGC 
TGCGCGGGGT GCTCACTCAA TCTTGGCTAC GTGTACAGAT GCACGCCCAA GAATCTTGAT 
TACAAGAGAG ACTTGTTTTG CCTCAGTGTT GAAGCCATTG AAAGGGAGAT GATGCCTGGA 
GCTGTGAAAC AAATCCTGAG CCACGGGATG AGTGTAGACT TGAGACTCCA GAGTTTGGGA 
TCCTGGTCCA GATCATGCCA CTGCTTACCA AGTAGGGTCC TGCCTACCAT AATCCAGGAG 
GTGGTCTTCC AGACATTAAA TAGGATCATG AAATCAATGG CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIS18A ( XM_017028401.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883890.1 Homo sapiens MIS18 kinetochore protein A (MIS18A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu93277
Clone ID Related Accession (Same CDS sequence)	XM_017028401.1
Accession Version	XM_017028401.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	protein Mis18-alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGCG TTCGGTCACT GAGGTGTAGC AGAGGATGCG CTGGCGGCTG TGAGTGCGGC 
GACAAGGGCA AATGCAGCGA CTCCTCGCTG TTGGGCAAGA GACTCTCCGA AGACTCGAGC 
CGCCACCAGC TGTTGCAGAA GTGGGCGAGC ATGTGGAGCT CCATGAGCGA AGACGCGTCG 
GTGGCCGACA TGGAGAGGGC GCAGCTGGAG GAGGAGGCGG CGGCTGCGGA GGAGAGGCCG 
CTGGTGTTCC TGTGCTCCGG CTGCCGGCGG CCGCTGGGCG ACTCGCTGAG CTGGGTGGCC 
AGCCAGGAGG ACACCAACTG CATCCTGCTT CGCTGTGTTT CCTGTAATGT TTCTGTGGAT 
AAGGAACAGA AGCTATCCAA ACGTGAAAAG GAAAATGGTT GCGTCCTTGA GACTTTGTGC 
TGCGCGGGGT GCTCACTCAA TCTTGGCTAC GTGTACAGAT GCACGCCCAA GAATCTTGAT 
TACAAGAGAG ACTTGTTTTG CCTCAGTGTT GAAGCCATTG AAAGGGAGAT GATGCCTGGA 
GCTGTGAAAC AAATCCTGAG CCACGGGATG AGTGTAGACT TGAGACTCCA GAGTTTGGGA 
TCCTGGTCCA GATCATGCCA CTGCTTACCA AGGTCCTGCC TACCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883890.1
CDS	143..790
Translation	MAGVRSLRCSRGCAGGCECGDKGKCSDSSLLGKRLSEDSSRHQLLQKWASMWSSMSEDASVADMERAQLEEEAAAAEERPLVFLCSGCRRPLGDSLSWVASQEDTNCILLRCVSCNVSVDKEQKLSKREKENGCVLETLCCAGCSLNLGYVYRCTPKNLDYKRDLFCLSVEAIEREMMPGAVKQILSHGMSVDLRLQSLGSWSRSCHCLPRSCLP

Target ORF information:

RefSeq Version	XM_017028401.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MIS18 kinetochore protein A (MIS18A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028401.1

ORF Insert Sequence:

ATGGCAGGCG TTCGGTCACT GAGGTGTAGC AGAGGATGCG CTGGCGGCTG TGAGTGCGGC 
GACAAGGGCA AATGCAGCGA CTCCTCGCTG TTGGGCAAGA GACTCTCCGA AGACTCGAGC 
CGCCACCAGC TGTTGCAGAA GTGGGCGAGC ATGTGGAGCT CCATGAGCGA AGACGCGTCG 
GTGGCCGACA TGGAGAGGGC GCAGCTGGAG GAGGAGGCGG CGGCTGCGGA GGAGAGGCCG 
CTGGTGTTCC TGTGCTCCGG CTGCCGGCGG CCGCTGGGCG ACTCGCTGAG CTGGGTGGCC 
AGCCAGGAGG ACACCAACTG CATCCTGCTT CGCTGTGTTT CCTGTAATGT TTCTGTGGAT 
AAGGAACAGA AGCTATCCAA ACGTGAAAAG GAAAATGGTT GCGTCCTTGA GACTTTGTGC 
TGCGCGGGGT GCTCACTCAA TCTTGGCTAC GTGTACAGAT GCACGCCCAA GAATCTTGAT 
TACAAGAGAG ACTTGTTTTG CCTCAGTGTT GAAGCCATTG AAAGGGAGAT GATGCCTGGA 
GCTGTGAAAC AAATCCTGAG CCACGGGATG AGTGTAGACT TGAGACTCCA GAGTTTGGGA 
TCCTGGTCCA GATCATGCCA CTGCTTACCA AGGTCCTGCC TACCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIS18A ( XM_017028402.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883891.1 Homo sapiens MIS18 kinetochore protein A (MIS18A), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu93278
Clone ID Related Accession (Same CDS sequence)	XM_017028402.1
Accession Version	XM_017028402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	protein Mis18-alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGTG TTTCCTGTAA TGTTTCTGTG GATAAGGAAC AGAAGCTATC CAAACGTGAA 
AAGGAAAATG GTTGCGTCCT TGAGACTTTG TGCTGCGCGG GGTGCTCACT CAATCTTGGC 
TACGTGTACA GATGCACGCC CAAGAATCTT GATTACAAGA GAGACTTGTT TTGCCTCAGT 
GTTGAAGCCA TTGAAAGGGA GATGATGCCT GGAGCTGTGA AACAAATCCT GAGCCACGGG 
ATGAGTGTAG ACTTGAGACT CCAGAGTTTG GGATCCTGGT CCAGATCATG CCACTGCTTA 
CCAAGTAGGG TCCTGCCTAC CATAATCCAG GAGGTGGTCT TCCAGACATT AAATAGGATC 
ATGAAATCAA TGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883891.1
CDS	78..455
Translation	MEGVSCNVSVDKEQKLSKREKENGCVLETLCCAGCSLNLGYVYRCTPKNLDYKRDLFCLSVEAIEREMMPGAVKQILSHGMSVDLRLQSLGSWSRSCHCLPSRVLPTIIQEVVFQTLNRIMKSMA

Target ORF information:

RefSeq Version	XM_017028402.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MIS18 kinetochore protein A (MIS18A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028402.1

ORF Insert Sequence:

ATGGAAGGTG TTTCCTGTAA TGTTTCTGTG GATAAGGAAC AGAAGCTATC CAAACGTGAA 
AAGGAAAATG GTTGCGTCCT TGAGACTTTG TGCTGCGCGG GGTGCTCACT CAATCTTGGC 
TACGTGTACA GATGCACGCC CAAGAATCTT GATTACAAGA GAGACTTGTT TTGCCTCAGT 
GTTGAAGCCA TTGAAAGGGA GATGATGCCT GGAGCTGTGA AACAAATCCT GAGCCACGGG 
ATGAGTGTAG ACTTGAGACT CCAGAGTTTG GGATCCTGGT CCAGATCATG CCACTGCTTA 
CCAAGTAGGG TCCTGCCTAC CATAATCCAG GAGGTGGTCT TCCAGACATT AAATAGGATC 
ATGAAATCAA TGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIS18A ( NM_018944.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_061817.1 Homo sapiens MIS18 kinetochore protein A (MIS18A), mRNA.

Price & Availability↑

CloneID	OHu02346
Clone ID Related Accession (Same CDS sequence)	NM_018944.2 , NM_018944.3
Accession Version	NM_018944.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573833600000
Organism	Homo sapiens(human)
Product	protein Mis18-alpha
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB959520.1, BU616573.1, AK075281.1, BU567844.1 and AW962893.1. On Nov 22, 2018 this sequence version replaced NM_018944.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189658.199892.1, SRR1163655.36739.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000290130.4/ ENSP00000290130.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCAGGCG TTCGGTCACT GAGGTGTAGC AGAGGATGCG CTGGCGGCTG TGAGTGCGGC 
GACAAGGGCA AATGCAGCGA CTCCTCGCTG TTGGGCAAGA GACTCTCCGA AGACTCGAGC 
CGCCACCAGC TGTTGCAGAA GTGGGCGAGC ATGTGGAGCT CCATGAGCGA AGACGCGTCG 
GTGGCCGACA TGGAGAGGGC GCAGCTGGAG GAGGAGGCGG CGGCTGCGGA GGAGAGGCCG 
CTGGTGTTCC TGTGCTCCGG CTGCCGGCGG CCGCTGGGCG ACTCGCTGAG CTGGGTGGCC 
AGCCAGGAGG ACACCAACTG CATCCTGCTT CGCTGTGTTT CCTGTAATGT TTCTGTGGAT 
AAGGAACAGA AGCTATCCAA ACGTGAAAAG GAAAATGGTT GCGTCCTTGA GACTTTGTGC 
TGCGCGGGGT GCTCACTCAA TCTTGGCTAC GTGTACAGAT GCACGCCCAA GAATCTTGAT 
TACAAGAGAG ACTTGTTTTG CCTCAGTGTT GAAGCCATTG AAAGTTATGT TTTAGGGTCC 
TCTGAAAAGC AAATTGTGTC AGAAGATAAA GAGCTTTTTA ATCTTGAAAG CAGAGTTGAA 
ATAGAAAAGT CTCTAACACA GATGGAAGAT GTCTTGAAAG CATTACAAAT GAAGCTGTGG 
GAGGCCGAAT CCAAATTGTC CTTTGCCACT TGTAAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061817.1
CDS	36..737
Translation	MAGVRSLRCSRGCAGGCECGDKGKCSDSSLLGKRLSEDSSRHQLLQKWASMWSSMSEDASVADMERAQLEEEAAAAEERPLVFLCSGCRRPLGDSLSWVASQEDTNCILLRCVSCNVSVDKEQKLSKREKENGCVLETLCCAGCSLNLGYVYRCTPKNLDYKRDLFCLSVEAIESYVLGSSEKQIVSEDKELFNLESRVEIEKSLTQMEDVLKALQMKLWEAESKLSFATCKS

Target ORF information:

RefSeq Version	NM_018944.3
Organism	Homo sapiens(human)
Definition	Homo sapiens MIS18 kinetochore protein A (MIS18A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018944.3

ORF Insert Sequence:

ATGGCAGGCG TTCGGTCACT GAGGTGTAGC AGAGGATGCG CTGGCGGCTG TGAGTGCGGC 
GACAAGGGCA AATGCAGCGA CTCCTCGCTG TTGGGCAAGA GACTCTCCGA AGACTCGAGC 
CGCCACCAGC TGTTGCAGAA GTGGGCGAGC ATGTGGAGCT CCATGAGCGA AGACGCGTCG 
GTGGCCGACA TGGAGAGGGC GCAGCTGGAG GAGGAGGCGG CGGCTGCGGA GGAGAGGCCG 
CTGGTGTTCC TGTGCTCCGG CTGCCGGCGG CCGCTGGGCG ACTCGCTGAG CTGGGTGGCC 
AGCCAGGAGG ACACCAACTG CATCCTGCTT CGCTGTGTTT CCTGTAATGT TTCTGTGGAT 
AAGGAACAGA AGCTATCCAA ACGTGAAAAG GAAAATGGTT GCGTCCTTGA GACTTTGTGC 
TGCGCGGGGT GCTCACTCAA TCTTGGCTAC GTGTACAGAT GCACGCCCAA GAATCTTGAT 
TACAAGAGAG ACTTGTTTTG CCTCAGTGTT GAAGCCATTG AAAGTTATGT TTTAGGGTCC 
TCTGAAAAGC AAATTGTGTC AGAAGATAAA GAGCTTTTTA ATCTTGAAAG CAGAGTTGAA 
ATAGAAAAGT CTCTAACACA GATGGAAGAT GTCTTGAAAG CATTACAAAT GAAGCTGTGG 
GAGGCCGAAT CCAAATTGTC CTTTGCCACT TGTAAAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Skin-Specific Deletion of Mis18α Impedes Proliferation and Stratification of Epidermal Keratinocytes.
The Journal of investigative dermatology137(2)414-421(2017 02)
Park KC,Lee M,Jeon Y,Jeon R,Baek SH,Lee H,Kim KI

CDK-regulated dimerization of M18BP1 on a Mis18 hexamer is necessary for CENP-A loading.
eLife6(2017 01)
Pan D,Klare K,Petrovic A,Take A,Walstein K,Singh P,Rondelet A,Bird AW,Musacchio A

Differential Binding Partners of the Mis18α/β YIPPEE Domains Regulate Mis18 Complex Recruitment to Centromeres.
Cell reports15(10)2127-2135(2016 06)
Stellfox ME,Nardi IK,Knippler CM,Foltz DR

Licensing of Centromeric Chromatin Assembly through the Mis18α-Mis18β Heterotetramer.
Molecular cell61(5)774-787(2016 Mar)
Nardi IK,Zasadzińska E,Stellfox ME,Knippler CM,Foltz DR

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MIS18A cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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