ZFYVE1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ZFYVE1
Entrez Gene ID	53349
Full Name	zinc finger FYVE-type containing 1
Synonyms	DFCP1,PPP1R172,SR3,TAFF1,ZNFN2A1
General protein information	Preferred Names zinc finger FYVE-type containing 1 Names zinc finger FYVE domain-containing protein 1 double FYVE-containing protein 1 phosphoinositide-binding protein SR3 protein phosphatase 1, regulatory subunit 172 tandem FYVE fingers-1 protein zinc finger protein, subfamily 2A, member 1 zinc finger, FYVE domain containing 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q24.2
Summary	The FYVE domain mediates the recruitment of proteins involved in membrane trafficking and cell signaling to phosphatidylinositol 3-phosphate-containing membranes. This protein contains two zinc-binding FYVE domains in tandem and is reported to localize to the Golgi apparatus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013].
Disorder MIM:	605471

mRNA and Protein(s)

mRNA	Protein	Name
NM_001281734.1	NP_001268663.1	zinc finger FYVE domain-containing protein 1 isoform 3
NM_021260.3	NP_067083.1	zinc finger FYVE domain-containing protein 1 isoform 1
NM_021260.4	NP_067083.1	zinc finger FYVE domain-containing protein 1 isoform 1
NM_178441.2	NP_848535.1	zinc finger FYVE domain-containing protein 1 isoform 2
NM_001281735.1	NP_001268664.1	zinc finger FYVE domain-containing protein 1 isoform 2
NM_001281734.2	NP_001268663.1	zinc finger FYVE domain-containing protein 1 isoform 3
XM_017021373.1	XP_016876862.1	zinc finger FYVE domain-containing protein 1 isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	ZFYVE1	NP_001186615.1
Mus musculus (house mouse)	Zfyve1	NP_898977.2
Macaca mulatta (Rhesus monkey)	ZFYVE1	XP_001084952.1
Canis lupus familiaris (dog)	ZFYVE1	XP_005623681.1
Danio rerio (zebrafish)	zfyve1	XP_001344703.1
Homo sapiens (human)	ZFYVE1	NP_067083.1
Bos taurus (cattle)	ZFYVE1	NP_001193041.1
Rattus norvegicus (Norway rat)	Zfyve1	NP_001100213.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002036	XP_321014.5
Pan troglodytes (chimpanzee)	ZFYVE1	XP_522899.2
Xenopus tropicalis (tropical clawed frog)	zfyve1	XP_002936927.1

Gene Ontology
Bibliography

Related articles in PubMed

PI(5)P regulates autophagosome biogenesis.
Vicinanza M, Korolchuk VI, Ashkenazi A, Puri C, Menzies FM, Clarke JH, Rubinsztein DC
Molecular cell57(2)219-34(2015 Jan)

WIPI2 links LC3 conjugation with PI3P, autophagosome formation, and pathogen clearance by recruiting Atg12-5-16L1.
Dooley HC, Razi M, Polson HE, Girardin SE, Wilson MI, Tooze SA
Molecular cell55(2)238-52(2014 Jul)

Autophagosomes form at ER-mitochondria contact sites.
Hamasaki M, Furuta N, Matsuda A, Nezu A, Yamamoto A, Fujita N, Oomori H, Noda T, Haraguchi T, Hiraoka Y, Amano A, Yoshimori T
Nature495(7441)389-93(2013 Mar)

Dynamic and transient interactions of Atg9 with autophagosomes, but not membrane integration, are required for autophagy.
Orsi A, Razi M, Dooley HC, Robinson D, Weston AE, Collinson LM, Tooze SA
Molecular biology of the cell23(10)1860-73(2012 May)

A subdomain of the endoplasmic reticulum forms a cradle for autophagosome formation.
Hayashi-Nishino M, Fujita N, Noda T, Yamaguchi A, Yoshimori T, Yamamoto A
Nature cell biology11(12)1433-7(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ZFYVE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZFYVE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28177	NM_001281735.1 Latest version!	Homo sapiens zinc finger FYVE-type containing 1 (ZFYVE1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu28177	NM_178441.2 Latest version!	Homo sapiens zinc finger FYVE-type containing 1 (ZFYVE1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu28177	XM_017021373.1 Latest version!	Homo sapiens zinc finger FYVE-type containing 1 (ZFYVE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu28132	NM_021260.4 Latest version!	Homo sapiens zinc finger FYVE-type containing 1 (ZFYVE1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu28149	NM_001281734.2 Latest version!	Homo sapiens zinc finger FYVE-type containing 1 (ZFYVE1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu28132	NM_021260.3	Homo sapiens zinc finger FYVE-type containing 1 (ZFYVE1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu28149	NM_001281734.1	Homo sapiens zinc finger FYVE-type containing 1 (ZFYVE1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZFYVE1 ( NM_178441.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_848535.1 Homo sapiens zinc finger FYVE-type containing 1 (ZFYVE1), transcript variant 2, mRNA.

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CloneID	OHu28177
Clone ID Related Accession (Same CDS sequence)	NM_178441.2 , NM_001281735.1 , XM_017021373.1
Accession Version	NM_178441.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564848000000
Organism	Homo sapiens(human)
Product	zinc finger FYVE domain-containing protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF311602.1, AI073571.1, AW070816.1, CK005173.1, BX339655.2 and HY248287.1. On Aug 1, 2013 this sequence version replaced NM_178441.1. Transcript Variant: This variant (2) uses an alternate 5' exon structure, and thus differs in the 5' UTR and 5' coding region, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus, compared to isoform 1. Both variants 2 and 4 encode the same isoform (2). ##Evidence-Data-START## Transcript exon combination :: AF311602.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCACA GCTCCTTTTT TCCAGATGAG TATTTCACCT GCTCCTCCTT GTGCCTCAGC 
TGTGGGGTTG GATGTAAGAA AAGCATGAAT CATGGGAAGG AAGGAGTGCC TCATGAAGCC 
AAGAGCCGCT GCAGATACTC CCACCAGTAT GACAACCGAG TGTATACCTG CAAGGCCTGC 
TATGAGAGAG GCGAGGAAGT CAGTGTAGTG CCCAAAACAT CTGCTTCCAC TGACTCCCCC 
TGGATGGGTC TCGCAAAATA TGCCTGGTCT GGGTATGTGA TCGAATGTCC TAACTGTGGC 
GTGGTCTATC GTAGTCGGCA GTACTGGTTT GGAAACCAAG ATCCTGTGGA TACGGTGGTG 
CGGACAGAGA TTGTGCATGT GTGGCCTGGA ACTGATGGGT TTCTGAAGGA CAACAACAAT 
GCTGCCCAGC GCCTGTTGGA CGGGATGAAC TTCATGGCTC AGTCGGTGTC CGAGCTTAGC 
CTTGGACCCA CCAAGGCTGT GACTTCCTGG CTGACAGACC AGATCGCCCC TGCCTACTGG 
AGGCCCAACT CCCAGATTCT GAGCTGCAAC AAGTGTGCGA CGTCCTTTAA AGATAACGAC 
ACTAAGCATC ACTGCCGAGC CTGTGGGGAG GGCTTCTGTG ACAGCTGTTC ATCAAAGACT 
CGGCCAGTGC CTGAGCGGGG CTGGGGCCCT GCGCCAGTGC GGGTCTGTGA CAACTGCTAC 
GAAGCCAGGA ACGTCCAGTT AGCTGTTACC GAGGCACAAG TGGACGATGA AGGTGGAACG 
CTCATTGCTC GGAAGGTGGG CGAGGCCGTG CAGAACACTC TGGGAGCCGT GGTGACAGCC 
ATTGACATAC CACTAGGTCT GGTAAAGGAC GCGGCCAGGC CTGCGTACTG GGTGCCTGAC 
CACGAAATCC TCCACTGCCA CAACTGCCGG AAGGAGTTCA GCATCAAGCT CTCCAAGCAC 
CACTGCCGGG CCTGCGGACA GGGCTTCTGT GATGAGTGCT CCCATGACCG CCGGGCTGTT 
CCTTCTCGTG GCTGGGACCA TCCCGTCCGA GTCTGCTTCA ACTGCAATAA AAAGCCCGGT 
GACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_848535.1
CDS	108..1196
Translation	MAHSSFFPDEYFTCSSLCLSCGVGCKKSMNHGKEGVPHEAKSRCRYSHQYDNRVYTCKACYERGEEVSVVPKTSASTDSPWMGLAKYAWSGYVIECPNCGVVYRSRQYWFGNQDPVDTVVRTEIVHVWPGTDGFLKDNNNAAQRLLDGMNFMAQSVSELSLGPTKAVTSWLTDQIAPAYWRPNSQILSCNKCATSFKDNDTKHHCRACGEGFCDSCSSKTRPVPERGWGPAPVRVCDNCYEARNVQLAVTEAQVDDEGGTLIARKVGEAVQNTLGAVVTAIDIPLGLVKDAARPAYWVPDHEILHCHNCRKEFSIKLSKHHCRACGQGFCDECSHDRRAVPSRGWDHPVRVCFNCNKKPGDL

Target ORF information:

RefSeq Version	NM_178441.2
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger FYVE-type containing 1 (ZFYVE1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178441.2

ORF Insert Sequence:

ATGGCGCACA GCTCCTTTTT TCCAGATGAG TATTTCACCT GCTCCTCCTT GTGCCTCAGC 
TGTGGGGTTG GATGTAAGAA AAGCATGAAT CATGGGAAGG AAGGAGTGCC TCATGAAGCC 
AAGAGCCGCT GCAGATACTC CCACCAGTAT GACAACCGAG TGTATACCTG CAAGGCCTGC 
TATGAGAGAG GCGAGGAAGT CAGTGTAGTG CCCAAAACAT CTGCTTCCAC TGACTCCCCC 
TGGATGGGTC TCGCAAAATA TGCCTGGTCT GGGTATGTGA TCGAATGTCC TAACTGTGGC 
GTGGTCTATC GTAGTCGGCA GTACTGGTTT GGAAACCAAG ATCCTGTGGA TACGGTGGTG 
CGGACAGAGA TTGTGCATGT GTGGCCTGGA ACTGATGGGT TTCTGAAGGA CAACAACAAT 
GCTGCCCAGC GCCTGTTGGA CGGGATGAAC TTCATGGCTC AGTCGGTGTC CGAGCTTAGC 
CTTGGACCCA CCAAGGCTGT GACTTCCTGG CTGACAGACC AGATCGCCCC TGCCTACTGG 
AGGCCCAACT CCCAGATTCT GAGCTGCAAC AAGTGTGCGA CGTCCTTTAA AGATAACGAC 
ACTAAGCATC ACTGCCGAGC CTGTGGGGAG GGCTTCTGTG ACAGCTGTTC ATCAAAGACT 
CGGCCAGTGC CTGAGCGGGG CTGGGGCCCT GCGCCAGTGC GGGTCTGTGA CAACTGCTAC 
GAAGCCAGGA ACGTCCAGTT AGCTGTTACC GAGGCACAAG TGGACGATGA AGGTGGAACG 
CTCATTGCTC GGAAGGTGGG CGAGGCCGTG CAGAACACTC TGGGAGCCGT GGTGACAGCC 
ATTGACATAC CACTAGGTCT GGTAAAGGAC GCGGCCAGGC CTGCGTACTG GGTGCCTGAC 
CACGAAATCC TCCACTGCCA CAACTGCCGG AAGGAGTTCA GCATCAAGCT CTCCAAGCAC 
CACTGCCGGG CCTGCGGACA GGGCTTCTGT GATGAGTGCT CCCATGACCG CCGGGCTGTT 
CCTTCTCGTG GCTGGGACCA TCCCGTCCGA GTCTGCTTCA ACTGCAATAA AAAGCCCGGT 
GACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZFYVE1 ( NM_001281735.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001268664.1 Homo sapiens zinc finger FYVE-type containing 1 (ZFYVE1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu28177
Clone ID Related Accession (Same CDS sequence)	NM_178441.2 , NM_001281735.1 , XM_017021373.1
Accession Version	NM_001281735.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564934400000
Organism	Homo sapiens(human)
Product	zinc finger FYVE domain-containing protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL442663.3, AK098542.1, BU740588.1, AW070816.1, CK005173.1, BX339655.2 and HY248287.1. Transcript Variant: This variant (4) uses an alternate 5' exon structure, and thus differs in the 5' UTR and 5' coding region, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus, compared to isoform 1. Both variants 2 and 4 encode the same isoform (2). ##Evidence-Data-START## Transcript exon combination :: AK098542.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCACA GCTCCTTTTT TCCAGATGAG TATTTCACCT GCTCCTCCTT GTGCCTCAGC 
TGTGGGGTTG GATGTAAGAA AAGCATGAAT CATGGGAAGG AAGGAGTGCC TCATGAAGCC 
AAGAGCCGCT GCAGATACTC CCACCAGTAT GACAACCGAG TGTATACCTG CAAGGCCTGC 
TATGAGAGAG GCGAGGAAGT CAGTGTAGTG CCCAAAACAT CTGCTTCCAC TGACTCCCCC 
TGGATGGGTC TCGCAAAATA TGCCTGGTCT GGGTATGTGA TCGAATGTCC TAACTGTGGC 
GTGGTCTATC GTAGTCGGCA GTACTGGTTT GGAAACCAAG ATCCTGTGGA TACGGTGGTG 
CGGACAGAGA TTGTGCATGT GTGGCCTGGA ACTGATGGGT TTCTGAAGGA CAACAACAAT 
GCTGCCCAGC GCCTGTTGGA CGGGATGAAC TTCATGGCTC AGTCGGTGTC CGAGCTTAGC 
CTTGGACCCA CCAAGGCTGT GACTTCCTGG CTGACAGACC AGATCGCCCC TGCCTACTGG 
AGGCCCAACT CCCAGATTCT GAGCTGCAAC AAGTGTGCGA CGTCCTTTAA AGATAACGAC 
ACTAAGCATC ACTGCCGAGC CTGTGGGGAG GGCTTCTGTG ACAGCTGTTC ATCAAAGACT 
CGGCCAGTGC CTGAGCGGGG CTGGGGCCCT GCGCCAGTGC GGGTCTGTGA CAACTGCTAC 
GAAGCCAGGA ACGTCCAGTT AGCTGTTACC GAGGCACAAG TGGACGATGA AGGTGGAACG 
CTCATTGCTC GGAAGGTGGG CGAGGCCGTG CAGAACACTC TGGGAGCCGT GGTGACAGCC 
ATTGACATAC CACTAGGTCT GGTAAAGGAC GCGGCCAGGC CTGCGTACTG GGTGCCTGAC 
CACGAAATCC TCCACTGCCA CAACTGCCGG AAGGAGTTCA GCATCAAGCT CTCCAAGCAC 
CACTGCCGGG CCTGCGGACA GGGCTTCTGT GATGAGTGCT CCCATGACCG CCGGGCTGTT 
CCTTCTCGTG GCTGGGACCA TCCCGTCCGA GTCTGCTTCA ACTGCAATAA AAAGCCCGGT 
GACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268664.1
CDS	103..1191
Translation	MAHSSFFPDEYFTCSSLCLSCGVGCKKSMNHGKEGVPHEAKSRCRYSHQYDNRVYTCKACYERGEEVSVVPKTSASTDSPWMGLAKYAWSGYVIECPNCGVVYRSRQYWFGNQDPVDTVVRTEIVHVWPGTDGFLKDNNNAAQRLLDGMNFMAQSVSELSLGPTKAVTSWLTDQIAPAYWRPNSQILSCNKCATSFKDNDTKHHCRACGEGFCDSCSSKTRPVPERGWGPAPVRVCDNCYEARNVQLAVTEAQVDDEGGTLIARKVGEAVQNTLGAVVTAIDIPLGLVKDAARPAYWVPDHEILHCHNCRKEFSIKLSKHHCRACGQGFCDECSHDRRAVPSRGWDHPVRVCFNCNKKPGDL

Target ORF information:

RefSeq Version	NM_001281735.1
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger FYVE-type containing 1 (ZFYVE1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281735.1

ORF Insert Sequence:

ATGGCGCACA GCTCCTTTTT TCCAGATGAG TATTTCACCT GCTCCTCCTT GTGCCTCAGC 
TGTGGGGTTG GATGTAAGAA AAGCATGAAT CATGGGAAGG AAGGAGTGCC TCATGAAGCC 
AAGAGCCGCT GCAGATACTC CCACCAGTAT GACAACCGAG TGTATACCTG CAAGGCCTGC 
TATGAGAGAG GCGAGGAAGT CAGTGTAGTG CCCAAAACAT CTGCTTCCAC TGACTCCCCC 
TGGATGGGTC TCGCAAAATA TGCCTGGTCT GGGTATGTGA TCGAATGTCC TAACTGTGGC 
GTGGTCTATC GTAGTCGGCA GTACTGGTTT GGAAACCAAG ATCCTGTGGA TACGGTGGTG 
CGGACAGAGA TTGTGCATGT GTGGCCTGGA ACTGATGGGT TTCTGAAGGA CAACAACAAT 
GCTGCCCAGC GCCTGTTGGA CGGGATGAAC TTCATGGCTC AGTCGGTGTC CGAGCTTAGC 
CTTGGACCCA CCAAGGCTGT GACTTCCTGG CTGACAGACC AGATCGCCCC TGCCTACTGG 
AGGCCCAACT CCCAGATTCT GAGCTGCAAC AAGTGTGCGA CGTCCTTTAA AGATAACGAC 
ACTAAGCATC ACTGCCGAGC CTGTGGGGAG GGCTTCTGTG ACAGCTGTTC ATCAAAGACT 
CGGCCAGTGC CTGAGCGGGG CTGGGGCCCT GCGCCAGTGC GGGTCTGTGA CAACTGCTAC 
GAAGCCAGGA ACGTCCAGTT AGCTGTTACC GAGGCACAAG TGGACGATGA AGGTGGAACG 
CTCATTGCTC GGAAGGTGGG CGAGGCCGTG CAGAACACTC TGGGAGCCGT GGTGACAGCC 
ATTGACATAC CACTAGGTCT GGTAAAGGAC GCGGCCAGGC CTGCGTACTG GGTGCCTGAC 
CACGAAATCC TCCACTGCCA CAACTGCCGG AAGGAGTTCA GCATCAAGCT CTCCAAGCAC 
CACTGCCGGG CCTGCGGACA GGGCTTCTGT GATGAGTGCT CCCATGACCG CCGGGCTGTT 
CCTTCTCGTG GCTGGGACCA TCCCGTCCGA GTCTGCTTCA ACTGCAATAA AAAGCCCGGT 
GACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZFYVE1 ( XM_017021373.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876862.1 Homo sapiens zinc finger FYVE-type containing 1 (ZFYVE1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu28177
Clone ID Related Accession (Same CDS sequence)	NM_178441.2 , NM_001281735.1 , XM_017021373.1
Accession Version	XM_017021373.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	zinc finger FYVE domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCACA GCTCCTTTTT TCCAGATGAG TATTTCACCT GCTCCTCCTT GTGCCTCAGC 
TGTGGGGTTG GATGTAAGAA AAGCATGAAT CATGGGAAGG AAGGAGTGCC TCATGAAGCC 
AAGAGCCGCT GCAGATACTC CCACCAGTAT GACAACCGAG TGTATACCTG CAAGGCCTGC 
TATGAGAGAG GCGAGGAAGT CAGTGTAGTG CCCAAAACAT CTGCTTCCAC TGACTCCCCC 
TGGATGGGTC TCGCAAAATA TGCCTGGTCT GGGTATGTGA TCGAATGTCC TAACTGTGGC 
GTGGTCTATC GTAGTCGGCA GTACTGGTTT GGAAACCAAG ATCCTGTGGA TACGGTGGTG 
CGGACAGAGA TTGTGCATGT GTGGCCTGGA ACTGATGGGT TTCTGAAGGA CAACAACAAT 
GCTGCCCAGC GCCTGTTGGA CGGGATGAAC TTCATGGCTC AGTCGGTGTC CGAGCTTAGC 
CTTGGACCCA CCAAGGCTGT GACTTCCTGG CTGACAGACC AGATCGCCCC TGCCTACTGG 
AGGCCCAACT CCCAGATTCT GAGCTGCAAC AAGTGTGCGA CGTCCTTTAA AGATAACGAC 
ACTAAGCATC ACTGCCGAGC CTGTGGGGAG GGCTTCTGTG ACAGCTGTTC ATCAAAGACT 
CGGCCAGTGC CTGAGCGGGG CTGGGGCCCT GCGCCAGTGC GGGTCTGTGA CAACTGCTAC 
GAAGCCAGGA ACGTCCAGTT AGCTGTTACC GAGGCACAAG TGGACGATGA AGGTGGAACG 
CTCATTGCTC GGAAGGTGGG CGAGGCCGTG CAGAACACTC TGGGAGCCGT GGTGACAGCC 
ATTGACATAC CACTAGGTCT GGTAAAGGAC GCGGCCAGGC CTGCGTACTG GGTGCCTGAC 
CACGAAATCC TCCACTGCCA CAACTGCCGG AAGGAGTTCA GCATCAAGCT CTCCAAGCAC 
CACTGCCGGG CCTGCGGACA GGGCTTCTGT GATGAGTGCT CCCATGACCG CCGGGCTGTT 
CCTTCTCGTG GCTGGGACCA TCCCGTCCGA GTCTGCTTCA ACTGCAATAA AAAGCCCGGT 
GACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876862.1
CDS	632..1720
Translation	MAHSSFFPDEYFTCSSLCLSCGVGCKKSMNHGKEGVPHEAKSRCRYSHQYDNRVYTCKACYERGEEVSVVPKTSASTDSPWMGLAKYAWSGYVIECPNCGVVYRSRQYWFGNQDPVDTVVRTEIVHVWPGTDGFLKDNNNAAQRLLDGMNFMAQSVSELSLGPTKAVTSWLTDQIAPAYWRPNSQILSCNKCATSFKDNDTKHHCRACGEGFCDSCSSKTRPVPERGWGPAPVRVCDNCYEARNVQLAVTEAQVDDEGGTLIARKVGEAVQNTLGAVVTAIDIPLGLVKDAARPAYWVPDHEILHCHNCRKEFSIKLSKHHCRACGQGFCDECSHDRRAVPSRGWDHPVRVCFNCNKKPGDL

Target ORF information:

RefSeq Version	XM_017021373.1
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger FYVE-type containing 1 (ZFYVE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021373.1

ORF Insert Sequence:

ATGGCGCACA GCTCCTTTTT TCCAGATGAG TATTTCACCT GCTCCTCCTT GTGCCTCAGC 
TGTGGGGTTG GATGTAAGAA AAGCATGAAT CATGGGAAGG AAGGAGTGCC TCATGAAGCC 
AAGAGCCGCT GCAGATACTC CCACCAGTAT GACAACCGAG TGTATACCTG CAAGGCCTGC 
TATGAGAGAG GCGAGGAAGT CAGTGTAGTG CCCAAAACAT CTGCTTCCAC TGACTCCCCC 
TGGATGGGTC TCGCAAAATA TGCCTGGTCT GGGTATGTGA TCGAATGTCC TAACTGTGGC 
GTGGTCTATC GTAGTCGGCA GTACTGGTTT GGAAACCAAG ATCCTGTGGA TACGGTGGTG 
CGGACAGAGA TTGTGCATGT GTGGCCTGGA ACTGATGGGT TTCTGAAGGA CAACAACAAT 
GCTGCCCAGC GCCTGTTGGA CGGGATGAAC TTCATGGCTC AGTCGGTGTC CGAGCTTAGC 
CTTGGACCCA CCAAGGCTGT GACTTCCTGG CTGACAGACC AGATCGCCCC TGCCTACTGG 
AGGCCCAACT CCCAGATTCT GAGCTGCAAC AAGTGTGCGA CGTCCTTTAA AGATAACGAC 
ACTAAGCATC ACTGCCGAGC CTGTGGGGAG GGCTTCTGTG ACAGCTGTTC ATCAAAGACT 
CGGCCAGTGC CTGAGCGGGG CTGGGGCCCT GCGCCAGTGC GGGTCTGTGA CAACTGCTAC 
GAAGCCAGGA ACGTCCAGTT AGCTGTTACC GAGGCACAAG TGGACGATGA AGGTGGAACG 
CTCATTGCTC GGAAGGTGGG CGAGGCCGTG CAGAACACTC TGGGAGCCGT GGTGACAGCC 
ATTGACATAC CACTAGGTCT GGTAAAGGAC GCGGCCAGGC CTGCGTACTG GGTGCCTGAC 
CACGAAATCC TCCACTGCCA CAACTGCCGG AAGGAGTTCA GCATCAAGCT CTCCAAGCAC 
CACTGCCGGG CCTGCGGACA GGGCTTCTGT GATGAGTGCT CCCATGACCG CCGGGCTGTT 
CCTTCTCGTG GCTGGGACCA TCCCGTCCGA GTCTGCTTCA ACTGCAATAA AAAGCCCGGT 
GACCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZFYVE1 ( NM_021260.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_067083.1 Homo sapiens zinc finger FYVE-type containing 1 (ZFYVE1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28132
Clone ID Related Accession (Same CDS sequence)	NM_021260.3 , NM_021260.4
Accession Version	NM_021260.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Homo sapiens(human)
Product	zinc finger FYVE domain-containing protein 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB059103.1, BC053520.1, AB046809.1 and HY248287.1. On Aug 1, 2013 this sequence version replaced NM_021260.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1803615.220990.1, BC053520.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGCCC AGACTTCCCC AGCAGAGAAG GGCCTGAATC CGGGGCTGAT GTGCCAGGAA 
AGTTACGCTT GCAGCGGGAC TGATGAAGCT ATCTTTGAGT GTGATGAGTG CTGCAGTCTG 
CAGTGTCTCC GCTGCGAGGA GGAGCTCCAT CGGCAGGAGC GCCTGAGAAA CCATGAGCGG 
ATAAGACTCA AACCTGGCCA TGTCCCTTAC TGTGACCTCT GCAAGGGTCT CAGTGGGCAT 
TTACCAGGTG TTAGGCAGAG GGCAATAGTG AGGTGCCAGA CCTGCAAAAT TAACTTGTGC 
CTGGAGTGCC AGAAGAGGAC TCATTCTGGG GGTAACAAAA GGAGACACCC TGTTACTGTG 
TACAATGTCA GTAATCTCCA GGAGTCACTG GAGGCAGAAG AGATGGATGA GGAGACCAAG 
AGGAAGAAGA TGACTGAGAA GGTTGTGAGT TTCCTCCTAG TAGACGAAAA TGAAGAAATT 
CAGGTAACAA ATGAAGAAGA CTTTATTAGA AAATTGGACT GCAAACCTGA TCAGCATCTG 
AAAGTGGTTT CCATTTTTGG AAATACTGGT GATGGAAAGT CTCATACTCT CAACCACACT 
TTCTTTTATG GTCGTGAAGT CTTTAAAACC TCCCCGACCC AGGAGTCCTG CACTGTGGGA 
GTGTGGGCAG CCTATGACCC AGTTCACAAA GTAGCAGTGA TCGATACGGA AGGGCTCCTG 
GGGGCCACCG TGAATCTAAG CCAGAGAACA CGGCTGCTGC TTAAGGTCCT GGCCATCTCA 
GACCTCGTCA TCTATCGAAC TCATGCAGAC CGGCTGCATA ACGACCTCTT CAAATTCCTT 
GGGGATGCCT CAGAAGCTTA TCTGAAGCAC TTCACCAAGG AGCTCAAGGC CACCACTGCT 
CGCTGTGGCC TGGATGTCCC TTTATCCACA CTGGGCCCTG CAGTTATCAT CTTCCATGAG 
ACCGTGCACA CCCAGCTACT GGGCTCTGAT CATCCCTCAG AGGTGCCAGA GAAGCTCATC 
CAGGACCGGT TCCGGAAGCT GGGCCGTTTC CCTGAAGCCT TTAGTTCCAT TCACTACAAG 
GGAACGAGGA CTTACAACCC TCCCACGGAC TTTTCTGGGC TTCGGCGTGC TTTGGAGCAG 
CTACTAGAGA ATAACACCAC CCGTTCTCCC CGGCACCCGG GAGTCATCTT CAAAGCCCTG 
AAGGCACTAA GTGACCGCTT CAGCGGTGAG ATCCCCGATG ACCAGATGGC GCACAGCTCC 
TTTTTTCCAG ATGAGTATTT CACCTGCTCC TCCTTGTGCC TCAGCTGTGG GGTTGGATGT 
AAGAAAAGCA TGAATCATGG GAAGGAAGGA GTGCCTCATG AAGCCAAGAG CCGCTGCAGA 
TACTCCCACC AGTATGACAA CCGAGTGTAT ACCTGCAAGG CCTGCTATGA GAGAGGCGAG 
GAAGTCAGTG TAGTGCCCAA AACATCTGCT TCCACTGACT CCCCCTGGAT GGGTCTCGCA 
AAATATGCCT GGTCTGGGTA TGTGATCGAA TGTCCTAACT GTGGCGTGGT CTATCGTAGT 
CGGCAGTACT GGTTTGGAAA CCAAGATCCT GTGGATACGG TGGTGCGGAC AGAGATTGTG 
CATGTGTGGC CTGGAACTGA TGGGTTTCTG AAGGACAACA ACAATGCTGC CCAGCGCCTG 
TTGGACGGGA TGAACTTCAT GGCTCAGTCG GTGTCCGAGC TTAGCCTTGG ACCCACCAAG 
GCTGTGACTT CCTGGCTGAC AGACCAGATC GCCCCTGCCT ACTGGAGGCC CAACTCCCAG 
ATTCTGAGCT GCAACAAGTG TGCGACGTCC TTTAAAGATA ACGACACTAA GCATCACTGC 
CGAGCCTGTG GGGAGGGCTT CTGTGACAGC TGTTCATCAA AGACTCGGCC AGTGCCTGAG 
CGGGGCTGGG GCCCTGCGCC AGTGCGGGTC TGTGACAACT GCTACGAAGC CAGGAACGTC 
CAGTTAGCTG TTACCGAGGC ACAAGTGGAC GATGAAGGTG GAACGCTCAT TGCTCGGAAG 
GTGGGCGAGG CCGTGCAGAA CACTCTGGGA GCCGTGGTGA CAGCCATTGA CATACCACTA 
GGTCTGGTAA AGGACGCGGC CAGGCCTGCG TACTGGGTGC CTGACCACGA AATCCTCCAC 
TGCCACAACT GCCGGAAGGA GTTCAGCATC AAGCTCTCCA AGCACCACTG CCGGGCCTGC 
GGACAGGGCT TCTGTGATGA GTGCTCCCAT GACCGCCGGG CTGTTCCTTC TCGTGGCTGG 
GACCATCCCG TCCGAGTCTG CTTCAACTGC AATAAAAAGC CCGGTGACCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067083.1
CDS	722..3055
Translation	MSAQTSPAEKGLNPGLMCQESYACSGTDEAIFECDECCSLQCLRCEEELHRQERLRNHERIRLKPGHVPYCDLCKGLSGHLPGVRQRAIVRCQTCKINLCLECQKRTHSGGNKRRHPVTVYNVSNLQESLEAEEMDEETKRKKMTEKVVSFLLVDENEEIQVTNEEDFIRKLDCKPDQHLKVVSIFGNTGDGKSHTLNHTFFYGREVFKTSPTQESCTVGVWAAYDPVHKVAVIDTEGLLGATVNLSQRTRLLLKVLAISDLVIYRTHADRLHNDLFKFLGDASEAYLKHFTKELKATTARCGLDVPLSTLGPAVIIFHETVHTQLLGSDHPSEVPEKLIQDRFRKLGRFPEAFSSIHYKGTRTYNPPTDFSGLRRALEQLLENNTTRSPRHPGVIFKALKALSDRFSGEIPDDQMAHSSFFPDEYFTCSSLCLSCGVGCKKSMNHGKEGVPHEAKSRCRYSHQYDNRVYTCKACYERGEEVSVVPKTSASTDSPWMGLAKYAWSGYVIECPNCGVVYRSRQYWFGNQDPVDTVVRTEIVHVWPGTDGFLKDNNNAAQRLLDGMNFMAQSVSELSLGPTKAVTSWLTDQIAPAYWRPNSQILSCNKCATSFKDNDTKHHCRACGEGFCDSCSSKTRPVPERGWGPAPVRVCDNCYEARNVQLAVTEAQVDDEGGTLIARKVGEAVQNTLGAVVTAIDIPLGLVKDAARPAYWVPDHEILHCHNCRKEFSIKLSKHHCRACGQGFCDECSHDRRAVPSRGWDHPVRVCFNCNKKPGDL

Target ORF information:

RefSeq Version	NM_021260.3
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger FYVE-type containing 1 (ZFYVE1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021260.3

ORF Insert Sequence:

ATGAGTGCCC AGACTTCCCC AGCAGAGAAG GGCCTGAATC CGGGGCTGAT GTGCCAGGAA 
AGTTACGCTT GCAGCGGGAC TGATGAAGCT ATCTTTGAGT GTGATGAGTG CTGCAGTCTG 
CAGTGTCTCC GCTGCGAGGA GGAGCTCCAT CGGCAGGAGC GCCTGAGAAA CCATGAGCGG 
ATAAGACTCA AACCTGGCCA TGTCCCTTAC TGTGACCTCT GCAAGGGTCT CAGTGGGCAT 
TTACCAGGTG TTAGGCAGAG GGCAATAGTG AGGTGCCAGA CCTGCAAAAT TAACTTGTGC 
CTGGAGTGCC AGAAGAGGAC TCATTCTGGG GGTAACAAAA GGAGACACCC TGTTACTGTG 
TACAATGTCA GTAATCTCCA GGAGTCACTG GAGGCAGAAG AGATGGATGA GGAGACCAAG 
AGGAAGAAGA TGACTGAGAA GGTTGTGAGT TTCCTCCTAG TAGACGAAAA TGAAGAAATT 
CAGGTAACAA ATGAAGAAGA CTTTATTAGA AAATTGGACT GCAAACCTGA TCAGCATCTG 
AAAGTGGTTT CCATTTTTGG AAATACTGGT GATGGAAAGT CTCATACTCT CAACCACACT 
TTCTTTTATG GTCGTGAAGT CTTTAAAACC TCCCCGACCC AGGAGTCCTG CACTGTGGGA 
GTGTGGGCAG CCTATGACCC AGTTCACAAA GTAGCAGTGA TCGATACGGA AGGGCTCCTG 
GGGGCCACCG TGAATCTAAG CCAGAGAACA CGGCTGCTGC TTAAGGTCCT GGCCATCTCA 
GACCTCGTCA TCTATCGAAC TCATGCAGAC CGGCTGCATA ACGACCTCTT CAAATTCCTT 
GGGGATGCCT CAGAAGCTTA TCTGAAGCAC TTCACCAAGG AGCTCAAGGC CACCACTGCT 
CGCTGTGGCC TGGATGTCCC TTTATCCACA CTGGGCCCTG CAGTTATCAT CTTCCATGAG 
ACCGTGCACA CCCAGCTACT GGGCTCTGAT CATCCCTCAG AGGTGCCAGA GAAGCTCATC 
CAGGACCGGT TCCGGAAGCT GGGCCGTTTC CCTGAAGCCT TTAGTTCCAT TCACTACAAG 
GGAACGAGGA CTTACAACCC TCCCACGGAC TTTTCTGGGC TTCGGCGTGC TTTGGAGCAG 
CTACTAGAGA ATAACACCAC CCGTTCTCCC CGGCACCCGG GAGTCATCTT CAAAGCCCTG 
AAGGCACTAA GTGACCGCTT CAGCGGTGAG ATCCCCGATG ACCAGATGGC GCACAGCTCC 
TTTTTTCCAG ATGAGTATTT CACCTGCTCC TCCTTGTGCC TCAGCTGTGG GGTTGGATGT 
AAGAAAAGCA TGAATCATGG GAAGGAAGGA GTGCCTCATG AAGCCAAGAG CCGCTGCAGA 
TACTCCCACC AGTATGACAA CCGAGTGTAT ACCTGCAAGG CCTGCTATGA GAGAGGCGAG 
GAAGTCAGTG TAGTGCCCAA AACATCTGCT TCCACTGACT CCCCCTGGAT GGGTCTCGCA 
AAATATGCCT GGTCTGGGTA TGTGATCGAA TGTCCTAACT GTGGCGTGGT CTATCGTAGT 
CGGCAGTACT GGTTTGGAAA CCAAGATCCT GTGGATACGG TGGTGCGGAC AGAGATTGTG 
CATGTGTGGC CTGGAACTGA TGGGTTTCTG AAGGACAACA ACAATGCTGC CCAGCGCCTG 
TTGGACGGGA TGAACTTCAT GGCTCAGTCG GTGTCCGAGC TTAGCCTTGG ACCCACCAAG 
GCTGTGACTT CCTGGCTGAC AGACCAGATC GCCCCTGCCT ACTGGAGGCC CAACTCCCAG 
ATTCTGAGCT GCAACAAGTG TGCGACGTCC TTTAAAGATA ACGACACTAA GCATCACTGC 
CGAGCCTGTG GGGAGGGCTT CTGTGACAGC TGTTCATCAA AGACTCGGCC AGTGCCTGAG 
CGGGGCTGGG GCCCTGCGCC AGTGCGGGTC TGTGACAACT GCTACGAAGC CAGGAACGTC 
CAGTTAGCTG TTACCGAGGC ACAAGTGGAC GATGAAGGTG GAACGCTCAT TGCTCGGAAG 
GTGGGCGAGG CCGTGCAGAA CACTCTGGGA GCCGTGGTGA CAGCCATTGA CATACCACTA 
GGTCTGGTAA AGGACGCGGC CAGGCCTGCG TACTGGGTGC CTGACCACGA AATCCTCCAC 
TGCCACAACT GCCGGAAGGA GTTCAGCATC AAGCTCTCCA AGCACCACTG CCGGGCCTGC 
GGACAGGGCT TCTGTGATGA GTGCTCCCAT GACCGCCGGG CTGTTCCTTC TCGTGGCTGG 
GACCATCCCG TCCGAGTCTG CTTCAACTGC AATAAAAAGC CCGGTGACCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZFYVE1 ( NM_021260.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_067083.1 Homo sapiens zinc finger FYVE-type containing 1 (ZFYVE1), transcript variant 1, mRNA.

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CloneID	OHu28132
Clone ID Related Accession (Same CDS sequence)	NM_021260.3 , NM_021260.4
Accession Version	NM_021260.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565020800000
Organism	Homo sapiens(human)
Product	zinc finger FYVE domain-containing protein 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC053520.1, AB046809.1 and HY248287.1. On Nov 22, 2018 this sequence version replaced NM_021260.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC053520.1, SRR1660805.194284.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000556143.6/ ENSP00000450742.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGTGCCC AGACTTCCCC AGCAGAGAAG GGCCTGAATC CGGGGCTGAT GTGCCAGGAA 
AGTTACGCTT GCAGCGGGAC TGATGAAGCT ATCTTTGAGT GTGATGAGTG CTGCAGTCTG 
CAGTGTCTCC GCTGCGAGGA GGAGCTCCAT CGGCAGGAGC GCCTGAGAAA CCATGAGCGG 
ATAAGACTCA AACCTGGCCA TGTCCCTTAC TGTGACCTCT GCAAGGGTCT CAGTGGGCAT 
TTACCAGGTG TTAGGCAGAG GGCAATAGTG AGGTGCCAGA CCTGCAAAAT TAACTTGTGC 
CTGGAGTGCC AGAAGAGGAC TCATTCTGGG GGTAACAAAA GGAGACACCC TGTTACTGTG 
TACAATGTCA GTAATCTCCA GGAGTCACTG GAGGCAGAAG AGATGGATGA GGAGACCAAG 
AGGAAGAAGA TGACTGAGAA GGTTGTGAGT TTCCTCCTAG TAGACGAAAA TGAAGAAATT 
CAGGTAACAA ATGAAGAAGA CTTTATTAGA AAATTGGACT GCAAACCTGA TCAGCATCTG 
AAAGTGGTTT CCATTTTTGG AAATACTGGT GATGGAAAGT CTCATACTCT CAACCACACT 
TTCTTTTATG GTCGTGAAGT CTTTAAAACC TCCCCGACCC AGGAGTCCTG CACTGTGGGA 
GTGTGGGCAG CCTATGACCC AGTTCACAAA GTAGCAGTGA TCGATACGGA AGGGCTCCTG 
GGGGCCACCG TGAATCTAAG CCAGAGAACA CGGCTGCTGC TTAAGGTCCT GGCCATCTCA 
GACCTCGTCA TCTATCGAAC TCATGCAGAC CGGCTGCATA ACGACCTCTT CAAATTCCTT 
GGGGATGCCT CAGAAGCTTA TCTGAAGCAC TTCACCAAGG AGCTCAAGGC CACCACTGCT 
CGCTGTGGCC TGGATGTCCC TTTATCCACA CTGGGCCCTG CAGTTATCAT CTTCCATGAG 
ACCGTGCACA CCCAGCTACT GGGCTCTGAT CATCCCTCAG AGGTGCCAGA GAAGCTCATC 
CAGGACCGGT TCCGGAAGCT GGGCCGTTTC CCTGAAGCCT TTAGTTCCAT TCACTACAAG 
GGAACGAGGA CTTACAACCC TCCCACGGAC TTTTCTGGGC TTCGGCGTGC TTTGGAGCAG 
CTACTAGAGA ATAACACCAC CCGTTCTCCC CGGCACCCGG GAGTCATCTT CAAAGCCCTG 
AAGGCACTAA GTGACCGCTT CAGCGGTGAG ATCCCCGATG ACCAGATGGC GCACAGCTCC 
TTTTTTCCAG ATGAGTATTT CACCTGCTCC TCCTTGTGCC TCAGCTGTGG GGTTGGATGT 
AAGAAAAGCA TGAATCATGG GAAGGAAGGA GTGCCTCATG AAGCCAAGAG CCGCTGCAGA 
TACTCCCACC AGTATGACAA CCGAGTGTAT ACCTGCAAGG CCTGCTATGA GAGAGGCGAG 
GAAGTCAGTG TAGTGCCCAA AACATCTGCT TCCACTGACT CCCCCTGGAT GGGTCTCGCA 
AAATATGCCT GGTCTGGGTA TGTGATCGAA TGTCCTAACT GTGGCGTGGT CTATCGTAGT 
CGGCAGTACT GGTTTGGAAA CCAAGATCCT GTGGATACGG TGGTGCGGAC AGAGATTGTG 
CATGTGTGGC CTGGAACTGA TGGGTTTCTG AAGGACAACA ACAATGCTGC CCAGCGCCTG 
TTGGACGGGA TGAACTTCAT GGCTCAGTCG GTGTCCGAGC TTAGCCTTGG ACCCACCAAG 
GCTGTGACTT CCTGGCTGAC AGACCAGATC GCCCCTGCCT ACTGGAGGCC CAACTCCCAG 
ATTCTGAGCT GCAACAAGTG TGCGACGTCC TTTAAAGATA ACGACACTAA GCATCACTGC 
CGAGCCTGTG GGGAGGGCTT CTGTGACAGC TGTTCATCAA AGACTCGGCC AGTGCCTGAG 
CGGGGCTGGG GCCCTGCGCC AGTGCGGGTC TGTGACAACT GCTACGAAGC CAGGAACGTC 
CAGTTAGCTG TTACCGAGGC ACAAGTGGAC GATGAAGGTG GAACGCTCAT TGCTCGGAAG 
GTGGGCGAGG CCGTGCAGAA CACTCTGGGA GCCGTGGTGA CAGCCATTGA CATACCACTA 
GGTCTGGTAA AGGACGCGGC CAGGCCTGCG TACTGGGTGC CTGACCACGA AATCCTCCAC 
TGCCACAACT GCCGGAAGGA GTTCAGCATC AAGCTCTCCA AGCACCACTG CCGGGCCTGC 
GGACAGGGCT TCTGTGATGA GTGCTCCCAT GACCGCCGGG CTGTTCCTTC TCGTGGCTGG 
GACCATCCCG TCCGAGTCTG CTTCAACTGC AATAAAAAGC CCGGTGACCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067083.1
CDS	616..2949
Translation	MSAQTSPAEKGLNPGLMCQESYACSGTDEAIFECDECCSLQCLRCEEELHRQERLRNHERIRLKPGHVPYCDLCKGLSGHLPGVRQRAIVRCQTCKINLCLECQKRTHSGGNKRRHPVTVYNVSNLQESLEAEEMDEETKRKKMTEKVVSFLLVDENEEIQVTNEEDFIRKLDCKPDQHLKVVSIFGNTGDGKSHTLNHTFFYGREVFKTSPTQESCTVGVWAAYDPVHKVAVIDTEGLLGATVNLSQRTRLLLKVLAISDLVIYRTHADRLHNDLFKFLGDASEAYLKHFTKELKATTARCGLDVPLSTLGPAVIIFHETVHTQLLGSDHPSEVPEKLIQDRFRKLGRFPEAFSSIHYKGTRTYNPPTDFSGLRRALEQLLENNTTRSPRHPGVIFKALKALSDRFSGEIPDDQMAHSSFFPDEYFTCSSLCLSCGVGCKKSMNHGKEGVPHEAKSRCRYSHQYDNRVYTCKACYERGEEVSVVPKTSASTDSPWMGLAKYAWSGYVIECPNCGVVYRSRQYWFGNQDPVDTVVRTEIVHVWPGTDGFLKDNNNAAQRLLDGMNFMAQSVSELSLGPTKAVTSWLTDQIAPAYWRPNSQILSCNKCATSFKDNDTKHHCRACGEGFCDSCSSKTRPVPERGWGPAPVRVCDNCYEARNVQLAVTEAQVDDEGGTLIARKVGEAVQNTLGAVVTAIDIPLGLVKDAARPAYWVPDHEILHCHNCRKEFSIKLSKHHCRACGQGFCDECSHDRRAVPSRGWDHPVRVCFNCNKKPGDL

Target ORF information:

RefSeq Version	NM_021260.4
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger FYVE-type containing 1 (ZFYVE1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021260.4

ORF Insert Sequence:

ATGAGTGCCC AGACTTCCCC AGCAGAGAAG GGCCTGAATC CGGGGCTGAT GTGCCAGGAA 
AGTTACGCTT GCAGCGGGAC TGATGAAGCT ATCTTTGAGT GTGATGAGTG CTGCAGTCTG 
CAGTGTCTCC GCTGCGAGGA GGAGCTCCAT CGGCAGGAGC GCCTGAGAAA CCATGAGCGG 
ATAAGACTCA AACCTGGCCA TGTCCCTTAC TGTGACCTCT GCAAGGGTCT CAGTGGGCAT 
TTACCAGGTG TTAGGCAGAG GGCAATAGTG AGGTGCCAGA CCTGCAAAAT TAACTTGTGC 
CTGGAGTGCC AGAAGAGGAC TCATTCTGGG GGTAACAAAA GGAGACACCC TGTTACTGTG 
TACAATGTCA GTAATCTCCA GGAGTCACTG GAGGCAGAAG AGATGGATGA GGAGACCAAG 
AGGAAGAAGA TGACTGAGAA GGTTGTGAGT TTCCTCCTAG TAGACGAAAA TGAAGAAATT 
CAGGTAACAA ATGAAGAAGA CTTTATTAGA AAATTGGACT GCAAACCTGA TCAGCATCTG 
AAAGTGGTTT CCATTTTTGG AAATACTGGT GATGGAAAGT CTCATACTCT CAACCACACT 
TTCTTTTATG GTCGTGAAGT CTTTAAAACC TCCCCGACCC AGGAGTCCTG CACTGTGGGA 
GTGTGGGCAG CCTATGACCC AGTTCACAAA GTAGCAGTGA TCGATACGGA AGGGCTCCTG 
GGGGCCACCG TGAATCTAAG CCAGAGAACA CGGCTGCTGC TTAAGGTCCT GGCCATCTCA 
GACCTCGTCA TCTATCGAAC TCATGCAGAC CGGCTGCATA ACGACCTCTT CAAATTCCTT 
GGGGATGCCT CAGAAGCTTA TCTGAAGCAC TTCACCAAGG AGCTCAAGGC CACCACTGCT 
CGCTGTGGCC TGGATGTCCC TTTATCCACA CTGGGCCCTG CAGTTATCAT CTTCCATGAG 
ACCGTGCACA CCCAGCTACT GGGCTCTGAT CATCCCTCAG AGGTGCCAGA GAAGCTCATC 
CAGGACCGGT TCCGGAAGCT GGGCCGTTTC CCTGAAGCCT TTAGTTCCAT TCACTACAAG 
GGAACGAGGA CTTACAACCC TCCCACGGAC TTTTCTGGGC TTCGGCGTGC TTTGGAGCAG 
CTACTAGAGA ATAACACCAC CCGTTCTCCC CGGCACCCGG GAGTCATCTT CAAAGCCCTG 
AAGGCACTAA GTGACCGCTT CAGCGGTGAG ATCCCCGATG ACCAGATGGC GCACAGCTCC 
TTTTTTCCAG ATGAGTATTT CACCTGCTCC TCCTTGTGCC TCAGCTGTGG GGTTGGATGT 
AAGAAAAGCA TGAATCATGG GAAGGAAGGA GTGCCTCATG AAGCCAAGAG CCGCTGCAGA 
TACTCCCACC AGTATGACAA CCGAGTGTAT ACCTGCAAGG CCTGCTATGA GAGAGGCGAG 
GAAGTCAGTG TAGTGCCCAA AACATCTGCT TCCACTGACT CCCCCTGGAT GGGTCTCGCA 
AAATATGCCT GGTCTGGGTA TGTGATCGAA TGTCCTAACT GTGGCGTGGT CTATCGTAGT 
CGGCAGTACT GGTTTGGAAA CCAAGATCCT GTGGATACGG TGGTGCGGAC AGAGATTGTG 
CATGTGTGGC CTGGAACTGA TGGGTTTCTG AAGGACAACA ACAATGCTGC CCAGCGCCTG 
TTGGACGGGA TGAACTTCAT GGCTCAGTCG GTGTCCGAGC TTAGCCTTGG ACCCACCAAG 
GCTGTGACTT CCTGGCTGAC AGACCAGATC GCCCCTGCCT ACTGGAGGCC CAACTCCCAG 
ATTCTGAGCT GCAACAAGTG TGCGACGTCC TTTAAAGATA ACGACACTAA GCATCACTGC 
CGAGCCTGTG GGGAGGGCTT CTGTGACAGC TGTTCATCAA AGACTCGGCC AGTGCCTGAG 
CGGGGCTGGG GCCCTGCGCC AGTGCGGGTC TGTGACAACT GCTACGAAGC CAGGAACGTC 
CAGTTAGCTG TTACCGAGGC ACAAGTGGAC GATGAAGGTG GAACGCTCAT TGCTCGGAAG 
GTGGGCGAGG CCGTGCAGAA CACTCTGGGA GCCGTGGTGA CAGCCATTGA CATACCACTA 
GGTCTGGTAA AGGACGCGGC CAGGCCTGCG TACTGGGTGC CTGACCACGA AATCCTCCAC 
TGCCACAACT GCCGGAAGGA GTTCAGCATC AAGCTCTCCA AGCACCACTG CCGGGCCTGC 
GGACAGGGCT TCTGTGATGA GTGCTCCCAT GACCGCCGGG CTGTTCCTTC TCGTGGCTGG 
GACCATCCCG TCCGAGTCTG CTTCAACTGC AATAAAAAGC CCGGTGACCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZFYVE1 ( NM_001281734.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001268663.1 Homo sapiens zinc finger FYVE-type containing 1 (ZFYVE1), transcript variant 3, mRNA.

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CloneID	OHu28149
Clone ID Related Accession (Same CDS sequence)	NM_001281734.1 , NM_001281734.2
Accession Version	NM_001281734.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1530201600000
Organism	Homo sapiens(human)
Product	zinc finger FYVE domain-containing protein 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB059103.1, DA324499.1, EL734498.1, BC014902.1, AL442663.3 and HY248287.1. On Aug 31, 2013 this sequence version replaced XM_005267766.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC014902.1, SRR1803617.144186.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGCCC AGACTTCCCC AGCAGAGAAG GGCCTGAATC CGGGGCTGAT GTGCCAGGAA 
AGTTACGCTT GCAGCGGGAC TGATGAAGCT ATCTTTGAGT GTGATGAGTG CTGCAGTCTG 
CAGTGTCTCC GCTGCGAGGA GGAGCTCCAT CGGCAGGAGC GCCTGAGAAA CCATGAGCGG 
ATAAGACTCA AACCTGGCCA TGTCCCTTAC TGTGACCTCT GCAAGGGTCT CAGTGGGCAT 
TTACCAGGTG TTAGGCAGAG GGCAATAGTG AGGTGCCAGA CCTGCAAAAT TAACTTGTGC 
CTGGAGTGCC AGAAGAGGAC TCATTCTGGG GGTAACAAAA GGAGACACCC TGTTACTGTG 
TACAATGTCA GTAATCTCCA GGAGTCACTG GAGGCAGAAG AGATGGATGA GGAGACCAAG 
AGGAAGAAGA TGACTGAGAA GGTTGTGAGT TTCCTCCTAG TAGACGAAAA TGAAGAAATT 
CAGGTAACAA ATGAAGAAGA CTTTATTAGA AAATTGGACT GCAAACCTGA TCAGCATCTG 
AAAGTGGTTT CCATTTTTGG AAATACTGGT GATGGAAAGT CTCATACTCT CAACCACACT 
TTCTTTTATG GTCGTGAAGT CTTTAAAACC TCCCCGACCC AGGAGTCCTG CACTGTGGGA 
GTGTGGGCAG CCTATGACCC AGTTCACAAA GTAGCAGTGA TCGATACGGA AGGGCTCCTG 
GGGGCCACCG TGAATCTAAG CCAGAGAACA CGGCTGCTGC TTAAGGTCCT GGCCATCTCA 
GACCTCGTCA TCTATCGAAC TCATGCAGAC CGGCTGCATA ACGACCTCTT CAAATTCCTT 
GGGGATGCCT CAGAAGCTTA TCTGAAGCAC TTCACCAAGG AGCTCAAGGC CACCACTGCT 
CGCTGTGGCC TGGATGTCCC TTTATCCACA CTGGGCCCTG CAGTTATCAT CTTCCATGAG 
ACCGTGCACA CCCAGCTACT GGGCTCTGAT CATCCCTCAG AGGTGCCAGA GAAGCTCATC 
CAGGACCGGT TCCGGAAGCT GGGCCGTTTC CCTGAAGCCT TTAGTTCCAT TCACTACAAG 
GGAACGAGGA CTTACAACCC TCCCACGGAC TTTTCTGGGC TTCGGCGTGC TTTGGAGCAG 
CTACTAGAGA ATAACACCAC CCGTTCTCCC CGGCACCCGG GAGTCATCTT CAAAGCCCTG 
AAGGCACTAA GTGACCGCTT CAGCGGTGAG ATCCCCGATG ACCAGATGGC GCACAGCTCC 
TTTTTTCCAG ATGAGTATTT CACCTGCTCC TCCTTGTGCC TCAGCTGTGG GGTTGGATGT 
AAGAAAAGCA TGAATCATGG GAAGGAAGGA GTGCCTCATG AAGCCAAGAG CCGCTGCAGA 
TACTCCCACC AGTATGACAA CCGAGTGTAT ACCTGCAAGG CCTGCTATGA GAGAGGCGAG 
GAAGTCAGTG TAGTGCCCAA AACATCTGCT TCCACTGACT CCCCCTGGAT GGGTCTCGCA 
AAATATGCCT GGTCTGGTCG GCAGTACTGG TTTGGAAACC AAGATCCTGT GGATACGGTG 
GTGCGGACAG AGATTGTGCA TGTGTGGCCT GGAACTGATG GGTTTCTGAA GGACAACAAC 
AATGCTGCCC AGCGCCTGTT GGACGGGATG AACTTCATGG CTCAGTCGGT GTCCGAGCTT 
AGCCTTGGAC CCACCAAGGC TGTGACTTCC TGGCTGACAG ACCAGATCGC CCCTGCCTAC 
TGGAGGCCCA ACTCCCAGAT TCTGAGCTGC AACAAGTGTG CGACGTCCTT TAAAGATAAC 
GACACTAAGC ATCACTGCCG AGCCTGTGGG GAGGGCTTCT GTGACAGCTG TTCATCAAAG 
ACTCGGCCAG TGCCTGAGCG GGGCTGGGGC CCTGCGCCAG TGCGGGTCTG TGACAACTGC 
TACGAAGCCA GGAACGTCCA GTTAGCTGTT ACCGAGGCAC AAGTGGACGA TGAAGGTGGA 
ACGCTCATTG CTCGGAAGGT GGGCGAGGCC GTGCAGAACA CTCTGGGAGC CGTGGTGACA 
GCCATTGACA TACCACTAGG TCTGGTAAAG GACGCGGCCA GGCCTGCGTA CTGGGTGCCT 
GACCACGAAA TCCTCCACTG CCACAACTGC CGGAAGGAGT TCAGCATCAA GCTCTCCAAG 
CACCACTGCC GGGCCTGCGG ACAGGGCTTC TGTGATGAGT GCTCCCATGA CCGCCGGGCT 
GTTCCTTCTC GTGGCTGGGA CCATCCCGTC CGAGTCTGCT TCAACTGCAA TAAAAAGCCC 
GGTGACCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268663.1
CDS	722..3013
Translation	MSAQTSPAEKGLNPGLMCQESYACSGTDEAIFECDECCSLQCLRCEEELHRQERLRNHERIRLKPGHVPYCDLCKGLSGHLPGVRQRAIVRCQTCKINLCLECQKRTHSGGNKRRHPVTVYNVSNLQESLEAEEMDEETKRKKMTEKVVSFLLVDENEEIQVTNEEDFIRKLDCKPDQHLKVVSIFGNTGDGKSHTLNHTFFYGREVFKTSPTQESCTVGVWAAYDPVHKVAVIDTEGLLGATVNLSQRTRLLLKVLAISDLVIYRTHADRLHNDLFKFLGDASEAYLKHFTKELKATTARCGLDVPLSTLGPAVIIFHETVHTQLLGSDHPSEVPEKLIQDRFRKLGRFPEAFSSIHYKGTRTYNPPTDFSGLRRALEQLLENNTTRSPRHPGVIFKALKALSDRFSGEIPDDQMAHSSFFPDEYFTCSSLCLSCGVGCKKSMNHGKEGVPHEAKSRCRYSHQYDNRVYTCKACYERGEEVSVVPKTSASTDSPWMGLAKYAWSGRQYWFGNQDPVDTVVRTEIVHVWPGTDGFLKDNNNAAQRLLDGMNFMAQSVSELSLGPTKAVTSWLTDQIAPAYWRPNSQILSCNKCATSFKDNDTKHHCRACGEGFCDSCSSKTRPVPERGWGPAPVRVCDNCYEARNVQLAVTEAQVDDEGGTLIARKVGEAVQNTLGAVVTAIDIPLGLVKDAARPAYWVPDHEILHCHNCRKEFSIKLSKHHCRACGQGFCDECSHDRRAVPSRGWDHPVRVCFNCNKKPGDL

Target ORF information:

RefSeq Version	NM_001281734.1
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger FYVE-type containing 1 (ZFYVE1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281734.1

ORF Insert Sequence:

ATGAGTGCCC AGACTTCCCC AGCAGAGAAG GGCCTGAATC CGGGGCTGAT GTGCCAGGAA 
AGTTACGCTT GCAGCGGGAC TGATGAAGCT ATCTTTGAGT GTGATGAGTG CTGCAGTCTG 
CAGTGTCTCC GCTGCGAGGA GGAGCTCCAT CGGCAGGAGC GCCTGAGAAA CCATGAGCGG 
ATAAGACTCA AACCTGGCCA TGTCCCTTAC TGTGACCTCT GCAAGGGTCT CAGTGGGCAT 
TTACCAGGTG TTAGGCAGAG GGCAATAGTG AGGTGCCAGA CCTGCAAAAT TAACTTGTGC 
CTGGAGTGCC AGAAGAGGAC TCATTCTGGG GGTAACAAAA GGAGACACCC TGTTACTGTG 
TACAATGTCA GTAATCTCCA GGAGTCACTG GAGGCAGAAG AGATGGATGA GGAGACCAAG 
AGGAAGAAGA TGACTGAGAA GGTTGTGAGT TTCCTCCTAG TAGACGAAAA TGAAGAAATT 
CAGGTAACAA ATGAAGAAGA CTTTATTAGA AAATTGGACT GCAAACCTGA TCAGCATCTG 
AAAGTGGTTT CCATTTTTGG AAATACTGGT GATGGAAAGT CTCATACTCT CAACCACACT 
TTCTTTTATG GTCGTGAAGT CTTTAAAACC TCCCCGACCC AGGAGTCCTG CACTGTGGGA 
GTGTGGGCAG CCTATGACCC AGTTCACAAA GTAGCAGTGA TCGATACGGA AGGGCTCCTG 
GGGGCCACCG TGAATCTAAG CCAGAGAACA CGGCTGCTGC TTAAGGTCCT GGCCATCTCA 
GACCTCGTCA TCTATCGAAC TCATGCAGAC CGGCTGCATA ACGACCTCTT CAAATTCCTT 
GGGGATGCCT CAGAAGCTTA TCTGAAGCAC TTCACCAAGG AGCTCAAGGC CACCACTGCT 
CGCTGTGGCC TGGATGTCCC TTTATCCACA CTGGGCCCTG CAGTTATCAT CTTCCATGAG 
ACCGTGCACA CCCAGCTACT GGGCTCTGAT CATCCCTCAG AGGTGCCAGA GAAGCTCATC 
CAGGACCGGT TCCGGAAGCT GGGCCGTTTC CCTGAAGCCT TTAGTTCCAT TCACTACAAG 
GGAACGAGGA CTTACAACCC TCCCACGGAC TTTTCTGGGC TTCGGCGTGC TTTGGAGCAG 
CTACTAGAGA ATAACACCAC CCGTTCTCCC CGGCACCCGG GAGTCATCTT CAAAGCCCTG 
AAGGCACTAA GTGACCGCTT CAGCGGTGAG ATCCCCGATG ACCAGATGGC GCACAGCTCC 
TTTTTTCCAG ATGAGTATTT CACCTGCTCC TCCTTGTGCC TCAGCTGTGG GGTTGGATGT 
AAGAAAAGCA TGAATCATGG GAAGGAAGGA GTGCCTCATG AAGCCAAGAG CCGCTGCAGA 
TACTCCCACC AGTATGACAA CCGAGTGTAT ACCTGCAAGG CCTGCTATGA GAGAGGCGAG 
GAAGTCAGTG TAGTGCCCAA AACATCTGCT TCCACTGACT CCCCCTGGAT GGGTCTCGCA 
AAATATGCCT GGTCTGGTCG GCAGTACTGG TTTGGAAACC AAGATCCTGT GGATACGGTG 
GTGCGGACAG AGATTGTGCA TGTGTGGCCT GGAACTGATG GGTTTCTGAA GGACAACAAC 
AATGCTGCCC AGCGCCTGTT GGACGGGATG AACTTCATGG CTCAGTCGGT GTCCGAGCTT 
AGCCTTGGAC CCACCAAGGC TGTGACTTCC TGGCTGACAG ACCAGATCGC CCCTGCCTAC 
TGGAGGCCCA ACTCCCAGAT TCTGAGCTGC AACAAGTGTG CGACGTCCTT TAAAGATAAC 
GACACTAAGC ATCACTGCCG AGCCTGTGGG GAGGGCTTCT GTGACAGCTG TTCATCAAAG 
ACTCGGCCAG TGCCTGAGCG GGGCTGGGGC CCTGCGCCAG TGCGGGTCTG TGACAACTGC 
TACGAAGCCA GGAACGTCCA GTTAGCTGTT ACCGAGGCAC AAGTGGACGA TGAAGGTGGA 
ACGCTCATTG CTCGGAAGGT GGGCGAGGCC GTGCAGAACA CTCTGGGAGC CGTGGTGACA 
GCCATTGACA TACCACTAGG TCTGGTAAAG GACGCGGCCA GGCCTGCGTA CTGGGTGCCT 
GACCACGAAA TCCTCCACTG CCACAACTGC CGGAAGGAGT TCAGCATCAA GCTCTCCAAG 
CACCACTGCC GGGCCTGCGG ACAGGGCTTC TGTGATGAGT GCTCCCATGA CCGCCGGGCT 
GTTCCTTCTC GTGGCTGGGA CCATCCCGTC CGAGTCTGCT TCAACTGCAA TAAAAAGCCC 
GGTGACCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZFYVE1 ( NM_001281734.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001268663.1 Homo sapiens zinc finger FYVE-type containing 1 (ZFYVE1), transcript variant 3, mRNA.

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CloneID	OHu28149
Clone ID Related Accession (Same CDS sequence)	NM_001281734.1 , NM_001281734.2
Accession Version	NM_001281734.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565193600000
Organism	Homo sapiens(human)
Product	zinc finger FYVE domain-containing protein 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB059103.1, DA324499.1, EL734498.1, BC014902.1 and AL442663.3. On Jun 1, 2019 this sequence version replaced NM_001281734.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC014902.1, SRR1803617.144186.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGCCC AGACTTCCCC AGCAGAGAAG GGCCTGAATC CGGGGCTGAT GTGCCAGGAA 
AGTTACGCTT GCAGCGGGAC TGATGAAGCT ATCTTTGAGT GTGATGAGTG CTGCAGTCTG 
CAGTGTCTCC GCTGCGAGGA GGAGCTCCAT CGGCAGGAGC GCCTGAGAAA CCATGAGCGG 
ATAAGACTCA AACCTGGCCA TGTCCCTTAC TGTGACCTCT GCAAGGGTCT CAGTGGGCAT 
TTACCAGGTG TTAGGCAGAG GGCAATAGTG AGGTGCCAGA CCTGCAAAAT TAACTTGTGC 
CTGGAGTGCC AGAAGAGGAC TCATTCTGGG GGTAACAAAA GGAGACACCC TGTTACTGTG 
TACAATGTCA GTAATCTCCA GGAGTCACTG GAGGCAGAAG AGATGGATGA GGAGACCAAG 
AGGAAGAAGA TGACTGAGAA GGTTGTGAGT TTCCTCCTAG TAGACGAAAA TGAAGAAATT 
CAGGTAACAA ATGAAGAAGA CTTTATTAGA AAATTGGACT GCAAACCTGA TCAGCATCTG 
AAAGTGGTTT CCATTTTTGG AAATACTGGT GATGGAAAGT CTCATACTCT CAACCACACT 
TTCTTTTATG GTCGTGAAGT CTTTAAAACC TCCCCGACCC AGGAGTCCTG CACTGTGGGA 
GTGTGGGCAG CCTATGACCC AGTTCACAAA GTAGCAGTGA TCGATACGGA AGGGCTCCTG 
GGGGCCACCG TGAATCTAAG CCAGAGAACA CGGCTGCTGC TTAAGGTCCT GGCCATCTCA 
GACCTCGTCA TCTATCGAAC TCATGCAGAC CGGCTGCATA ACGACCTCTT CAAATTCCTT 
GGGGATGCCT CAGAAGCTTA TCTGAAGCAC TTCACCAAGG AGCTCAAGGC CACCACTGCT 
CGCTGTGGCC TGGATGTCCC TTTATCCACA CTGGGCCCTG CAGTTATCAT CTTCCATGAG 
ACCGTGCACA CCCAGCTACT GGGCTCTGAT CATCCCTCAG AGGTGCCAGA GAAGCTCATC 
CAGGACCGGT TCCGGAAGCT GGGCCGTTTC CCTGAAGCCT TTAGTTCCAT TCACTACAAG 
GGAACGAGGA CTTACAACCC TCCCACGGAC TTTTCTGGGC TTCGGCGTGC TTTGGAGCAG 
CTACTAGAGA ATAACACCAC CCGTTCTCCC CGGCACCCGG GAGTCATCTT CAAAGCCCTG 
AAGGCACTAA GTGACCGCTT CAGCGGTGAG ATCCCCGATG ACCAGATGGC GCACAGCTCC 
TTTTTTCCAG ATGAGTATTT CACCTGCTCC TCCTTGTGCC TCAGCTGTGG GGTTGGATGT 
AAGAAAAGCA TGAATCATGG GAAGGAAGGA GTGCCTCATG AAGCCAAGAG CCGCTGCAGA 
TACTCCCACC AGTATGACAA CCGAGTGTAT ACCTGCAAGG CCTGCTATGA GAGAGGCGAG 
GAAGTCAGTG TAGTGCCCAA AACATCTGCT TCCACTGACT CCCCCTGGAT GGGTCTCGCA 
AAATATGCCT GGTCTGGTCG GCAGTACTGG TTTGGAAACC AAGATCCTGT GGATACGGTG 
GTGCGGACAG AGATTGTGCA TGTGTGGCCT GGAACTGATG GGTTTCTGAA GGACAACAAC 
AATGCTGCCC AGCGCCTGTT GGACGGGATG AACTTCATGG CTCAGTCGGT GTCCGAGCTT 
AGCCTTGGAC CCACCAAGGC TGTGACTTCC TGGCTGACAG ACCAGATCGC CCCTGCCTAC 
TGGAGGCCCA ACTCCCAGAT TCTGAGCTGC AACAAGTGTG CGACGTCCTT TAAAGATAAC 
GACACTAAGC ATCACTGCCG AGCCTGTGGG GAGGGCTTCT GTGACAGCTG TTCATCAAAG 
ACTCGGCCAG TGCCTGAGCG GGGCTGGGGC CCTGCGCCAG TGCGGGTCTG TGACAACTGC 
TACGAAGCCA GGAACGTCCA GTTAGCTGTT ACCGAGGCAC AAGTGGACGA TGAAGGTGGA 
ACGCTCATTG CTCGGAAGGT GGGCGAGGCC GTGCAGAACA CTCTGGGAGC CGTGGTGACA 
GCCATTGACA TACCACTAGG TCTGGTAAAG GACGCGGCCA GGCCTGCGTA CTGGGTGCCT 
GACCACGAAA TCCTCCACTG CCACAACTGC CGGAAGGAGT TCAGCATCAA GCTCTCCAAG 
CACCACTGCC GGGCCTGCGG ACAGGGCTTC TGTGATGAGT GCTCCCATGA CCGCCGGGCT 
GTTCCTTCTC GTGGCTGGGA CCATCCCGTC CGAGTCTGCT TCAACTGCAA TAAAAAGCCC 
GGTGACCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268663.1
CDS	616..2907
Translation	MSAQTSPAEKGLNPGLMCQESYACSGTDEAIFECDECCSLQCLRCEEELHRQERLRNHERIRLKPGHVPYCDLCKGLSGHLPGVRQRAIVRCQTCKINLCLECQKRTHSGGNKRRHPVTVYNVSNLQESLEAEEMDEETKRKKMTEKVVSFLLVDENEEIQVTNEEDFIRKLDCKPDQHLKVVSIFGNTGDGKSHTLNHTFFYGREVFKTSPTQESCTVGVWAAYDPVHKVAVIDTEGLLGATVNLSQRTRLLLKVLAISDLVIYRTHADRLHNDLFKFLGDASEAYLKHFTKELKATTARCGLDVPLSTLGPAVIIFHETVHTQLLGSDHPSEVPEKLIQDRFRKLGRFPEAFSSIHYKGTRTYNPPTDFSGLRRALEQLLENNTTRSPRHPGVIFKALKALSDRFSGEIPDDQMAHSSFFPDEYFTCSSLCLSCGVGCKKSMNHGKEGVPHEAKSRCRYSHQYDNRVYTCKACYERGEEVSVVPKTSASTDSPWMGLAKYAWSGRQYWFGNQDPVDTVVRTEIVHVWPGTDGFLKDNNNAAQRLLDGMNFMAQSVSELSLGPTKAVTSWLTDQIAPAYWRPNSQILSCNKCATSFKDNDTKHHCRACGEGFCDSCSSKTRPVPERGWGPAPVRVCDNCYEARNVQLAVTEAQVDDEGGTLIARKVGEAVQNTLGAVVTAIDIPLGLVKDAARPAYWVPDHEILHCHNCRKEFSIKLSKHHCRACGQGFCDECSHDRRAVPSRGWDHPVRVCFNCNKKPGDL

Target ORF information:

RefSeq Version	NM_001281734.2
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger FYVE-type containing 1 (ZFYVE1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281734.2

ORF Insert Sequence:

ATGAGTGCCC AGACTTCCCC AGCAGAGAAG GGCCTGAATC CGGGGCTGAT GTGCCAGGAA 
AGTTACGCTT GCAGCGGGAC TGATGAAGCT ATCTTTGAGT GTGATGAGTG CTGCAGTCTG 
CAGTGTCTCC GCTGCGAGGA GGAGCTCCAT CGGCAGGAGC GCCTGAGAAA CCATGAGCGG 
ATAAGACTCA AACCTGGCCA TGTCCCTTAC TGTGACCTCT GCAAGGGTCT CAGTGGGCAT 
TTACCAGGTG TTAGGCAGAG GGCAATAGTG AGGTGCCAGA CCTGCAAAAT TAACTTGTGC 
CTGGAGTGCC AGAAGAGGAC TCATTCTGGG GGTAACAAAA GGAGACACCC TGTTACTGTG 
TACAATGTCA GTAATCTCCA GGAGTCACTG GAGGCAGAAG AGATGGATGA GGAGACCAAG 
AGGAAGAAGA TGACTGAGAA GGTTGTGAGT TTCCTCCTAG TAGACGAAAA TGAAGAAATT 
CAGGTAACAA ATGAAGAAGA CTTTATTAGA AAATTGGACT GCAAACCTGA TCAGCATCTG 
AAAGTGGTTT CCATTTTTGG AAATACTGGT GATGGAAAGT CTCATACTCT CAACCACACT 
TTCTTTTATG GTCGTGAAGT CTTTAAAACC TCCCCGACCC AGGAGTCCTG CACTGTGGGA 
GTGTGGGCAG CCTATGACCC AGTTCACAAA GTAGCAGTGA TCGATACGGA AGGGCTCCTG 
GGGGCCACCG TGAATCTAAG CCAGAGAACA CGGCTGCTGC TTAAGGTCCT GGCCATCTCA 
GACCTCGTCA TCTATCGAAC TCATGCAGAC CGGCTGCATA ACGACCTCTT CAAATTCCTT 
GGGGATGCCT CAGAAGCTTA TCTGAAGCAC TTCACCAAGG AGCTCAAGGC CACCACTGCT 
CGCTGTGGCC TGGATGTCCC TTTATCCACA CTGGGCCCTG CAGTTATCAT CTTCCATGAG 
ACCGTGCACA CCCAGCTACT GGGCTCTGAT CATCCCTCAG AGGTGCCAGA GAAGCTCATC 
CAGGACCGGT TCCGGAAGCT GGGCCGTTTC CCTGAAGCCT TTAGTTCCAT TCACTACAAG 
GGAACGAGGA CTTACAACCC TCCCACGGAC TTTTCTGGGC TTCGGCGTGC TTTGGAGCAG 
CTACTAGAGA ATAACACCAC CCGTTCTCCC CGGCACCCGG GAGTCATCTT CAAAGCCCTG 
AAGGCACTAA GTGACCGCTT CAGCGGTGAG ATCCCCGATG ACCAGATGGC GCACAGCTCC 
TTTTTTCCAG ATGAGTATTT CACCTGCTCC TCCTTGTGCC TCAGCTGTGG GGTTGGATGT 
AAGAAAAGCA TGAATCATGG GAAGGAAGGA GTGCCTCATG AAGCCAAGAG CCGCTGCAGA 
TACTCCCACC AGTATGACAA CCGAGTGTAT ACCTGCAAGG CCTGCTATGA GAGAGGCGAG 
GAAGTCAGTG TAGTGCCCAA AACATCTGCT TCCACTGACT CCCCCTGGAT GGGTCTCGCA 
AAATATGCCT GGTCTGGTCG GCAGTACTGG TTTGGAAACC AAGATCCTGT GGATACGGTG 
GTGCGGACAG AGATTGTGCA TGTGTGGCCT GGAACTGATG GGTTTCTGAA GGACAACAAC 
AATGCTGCCC AGCGCCTGTT GGACGGGATG AACTTCATGG CTCAGTCGGT GTCCGAGCTT 
AGCCTTGGAC CCACCAAGGC TGTGACTTCC TGGCTGACAG ACCAGATCGC CCCTGCCTAC 
TGGAGGCCCA ACTCCCAGAT TCTGAGCTGC AACAAGTGTG CGACGTCCTT TAAAGATAAC 
GACACTAAGC ATCACTGCCG AGCCTGTGGG GAGGGCTTCT GTGACAGCTG TTCATCAAAG 
ACTCGGCCAG TGCCTGAGCG GGGCTGGGGC CCTGCGCCAG TGCGGGTCTG TGACAACTGC 
TACGAAGCCA GGAACGTCCA GTTAGCTGTT ACCGAGGCAC AAGTGGACGA TGAAGGTGGA 
ACGCTCATTG CTCGGAAGGT GGGCGAGGCC GTGCAGAACA CTCTGGGAGC CGTGGTGACA 
GCCATTGACA TACCACTAGG TCTGGTAAAG GACGCGGCCA GGCCTGCGTA CTGGGTGCCT 
GACCACGAAA TCCTCCACTG CCACAACTGC CGGAAGGAGT TCAGCATCAA GCTCTCCAAG 
CACCACTGCC GGGCCTGCGG ACAGGGCTTC TGTGATGAGT GCTCCCATGA CCGCCGGGCT 
GTTCCTTCTC GTGGCTGGGA CCATCCCGTC CGAGTCTGCT TCAACTGCAA TAAAAAGCCC 
GGTGACCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PI(5)P regulates autophagosome biogenesis.
Molecular cell57(2)219-34(2015 Jan)
Vicinanza M,Korolchuk VI,Ashkenazi A,Puri C,Menzies FM,Clarke JH,Rubinsztein DC

WIPI2 links LC3 conjugation with PI3P, autophagosome formation, and pathogen clearance by recruiting Atg12-5-16L1.
Molecular cell55(2)238-52(2014 Jul)
Dooley HC,Razi M,Polson HE,Girardin SE,Wilson MI,Tooze SA

Autophagosomes form at ER-mitochondria contact sites.
Nature495(7441)389-93(2013 Mar)
Hamasaki M,Furuta N,Matsuda A,Nezu A,Yamamoto A,Fujita N,Oomori H,Noda T,Haraguchi T,Hiraoka Y,Amano A,Yoshimori T

Dynamic and transient interactions of Atg9 with autophagosomes, but not membrane integration, are required for autophagy.
Molecular biology of the cell23(10)1860-73(2012 May)
Orsi A,Razi M,Dooley HC,Robinson D,Weston AE,Collinson LM,Tooze SA

A subdomain of the endoplasmic reticulum forms a cradle for autophagosome formation.
Nature cell biology11(12)1433-7(2009 Dec)
Hayashi-Nishino M,Fujita N,Noda T,Yamaguchi A,Yoshimori T,Yamamoto A

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ZFYVE1 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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