PAM16 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PAM16
Entrez Gene ID	51025
Full Name	presequence translocase associated motor 16
Synonyms	CGI-136,MAGMAS,SMDMDM,TIM16,TIMM16
General protein information	Preferred Names presequence translocase associated motor 16 Names mitochondrial import inner membrane translocase subunit TIM16 magmas-like protein mitochondria associated protein involved in granulocyte macrophage colony stimulating factor signal transduction mitochondria-associated granulocyte macrophage CSF-signaling molecule presequence translocase associated motor 16 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p13.3
Summary	This gene encodes a mitochondrial protein involved in granulocyte-macrophage colony-stimulating factor (GM-CSF) signaling. This protein also plays a role in the import of nuclear-encoded mitochondrial proteins into the mitochondrial matrix and may be important in reactive oxygen species (ROS) homeostasis. Mutations in this gene cause Megarbane-Dagher-Melike type spondylometaphyseal dysplasia, an early lethal skeletal dysplasia characterized by short stature, developmental delay and other skeletal abnormalities. [provided by RefSeq, May 2017].
Disorder MIM:	614336
Disorder Html:	Spondylometaphyseal dysplasia, Megarbane-Dagher-Melike type, 613320 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_016069.9	NP_057153.8	mitochondrial import inner membrane translocase subunit TIM16
NM_016069.10	NP_057153.8	mitochondrial import inner membrane translocase subunit TIM16
NM_016069.11	NP_057153.8	mitochondrial import inner membrane translocase subunit TIM16

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PAM16	NP_001003391.1
Danio rerio (zebrafish)	pam16	NP_957098.2
Rattus norvegicus (Norway rat)	Pam16	NP_001093606.2
Drosophila melanogaster (fruit fly)	blp	NP_524370.2
Xenopus tropicalis (tropical clawed frog)	pam16	NP_001004771.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP013212	XP_003436301.1
Macaca mulatta (Rhesus monkey)	PAM16	XP_001095989.1
Bos taurus (cattle)	PAM16	NP_001002893.1
Mus musculus (house mouse)	Pam16	NP_079847.1
Homo sapiens (human)	PAM16	NP_057153.8
Gallus gallus (chicken)	PAM16	NP_001004377.1
Caenorhabditis elegans (roundworm)	F45G2.8	NP_499775.1
Pan troglodytes (chimpanzee)	PAM16	XP_003315006.1

Gene Ontology
Bibliography

Related articles in PubMed

Magmas inhibition as a potential treatment strategy in malignant glioma.
Di K, Lomeli N, Bota DA, Das BC
Journal of neuro-oncology141(2)267-276(2019 Jan)

A novel homozygous PAM16 mutation in a patient with a milder phenotype and longer survival.
Moosa S, Fano V, Obregon MG, Altmüller J, Thiele H, Nürnberg P, Nishimura G, Wollnik B
American journal of medical genetics. Part A170(9)2436-9(2016 09)

Functional Diversity of Human Mitochondrial J-proteins Is Independent of Their Association with the Inner Membrane Presequence Translocase.
Sinha D, Srivastava S, D'Silva P
The Journal of biological chemistry291(33)17345-59(2016 08)

Magmas functions as a ROS regulator and provides cytoprotection against oxidative stress-mediated damages.
Srivastava S, Sinha D, Saha PP, Marthala H, D'Silva P
Cell death & disease5e1394(2014 Aug)

The impairment of MAGMAS function in human is responsible for a severe skeletal dysplasia.
Mehawej C, Delahodde A, Legeai-Mallet L, Delague V, Kaci N, Desvignes JP, Kibar Z, Capo-Chichi JM, Chouery E, Munnich A, Cormier-Daire V, Mégarbané A
PLoS genetics10(5)e1004311(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PAM16 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PAM16 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09343	NM_016069.11 Latest version!	Homo sapiens presequence translocase associated motor 16 (PAM16), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
View Old Accession Versions >>
OHu09343	NM_016069.9	Homo sapiens presequence translocase-associated motor 16 homolog (S. cerevisiae) (PAM16), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu09343	NM_016069.10	Homo sapiens presequence translocase associated motor 16 (PAM16), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PAM16 ( NM_016069.9 ) cDNA ORF clone, Homo sapiens(human) -> NP_057153.8 Homo sapiens presequence translocase-associated motor 16 homolog (S. cerevisiae) (PAM16), mRNA.

Price & Availability↑

CloneID	OHu09343
Clone ID Related Accession (Same CDS sequence)	NM_016069.9 , NM_016069.10 , NM_016069.11
Accession Version	NM_016069.9	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427644800000
Organism	Homo sapiens(Human)
Product	mitochondrial import inner membrane translocase subunit TIM16
Comment

ATGGCCAAGT ACCTGGCCCA GATCATTGTG ATGGGCGTGC AGGTGGTGGG CAGGGCCTTT 
GCACGGGCCT TGCGGCAGGA GTTTGCAGCC AGCCGGGCCG CAGCTGATGC CCGAGGACGC 
GCTGGACACC GGTCTGCAGC CGCTTCCAAC CTCTCCGGCC TCAGCCTCCA GGAGGCACAG 
CAGATTCTCA ACGTGTCCAA GCTGAGCCCT GAGGAGGTCC AGAAGAACTA TGAACACTTA 
TTTAAGGTGA ATGATAAATC CGTGGGTGGC TCCTTCTACC TGCAGTCAAA GGTGGTCCGC 
GCAAAGGAGC GCCTGGATGA GGAACTCAAA ATCCAGGCCC AGGAGGACAG AGAAAAAGGG 
CAGATGCCCC ATACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057153.8
Translation	MAKYLAQIIVMGVQVVGRAFARALRQEFAASRAAADARGRAGHRSAAASNLSGLSLQEAQQILNVSKLSPEEVQKNYEHLFKVNDKSVGGSFYLQSKVVRAKERLDEELKIQAQEDREKGQMPHT

Target ORF information:

RefSeq Version	NM_016069.9
Organism	Homo sapiens(Human)
Definition	Homo sapiens presequence translocase-associated motor 16 homolog (S. cerevisiae) (PAM16), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016069.9

ORF Insert Sequence:

ATGGCCAAGT ACCTGGCCCA GATCATTGTG ATGGGCGTGC AGGTGGTGGG CAGGGCCTTT 
GCACGGGCCT TGCGGCAGGA GTTTGCAGCC AGCCGGGCCG CAGCTGATGC CCGAGGACGC 
GCTGGACACC GGTCTGCAGC CGCTTCCAAC CTCTCCGGCC TCAGCCTCCA GGAGGCACAG 
CAGATTCTCA ACGTGTCCAA GCTGAGCCCT GAGGAGGTCC AGAAGAACTA TGAACACTTA 
TTTAAGGTGA ATGATAAATC CGTGGGTGGC TCCTTCTACC TGCAGTCAAA GGTGGTCCGC 
GCAAAGGAGC GCCTGGATGA GGAACTCAAA ATCCAGGCCC AGGAGGACAG AGAAAAAGGG 
CAGATGCCCC ATACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAM16 ( NM_016069.10 ) cDNA ORF clone, Homo sapiens(human) -> NP_057153.8 Homo sapiens presequence translocase associated motor 16 (PAM16), mRNA.

Price & Availability↑

CloneID	OHu09343
Clone ID Related Accession (Same CDS sequence)	NM_016069.9 , NM_016069.10 , NM_016069.11
Accession Version	NM_016069.10	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504886400000
Organism	Homo sapiens(human)
Product	mitochondrial import inner membrane translocase subunit TIM16
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012676.5, BG715429.1, AK026514.1 and BQ026935.1. This sequence is a reference standard in the RefSeqGene project. On May 2, 2017 this sequence version replaced NM_016069.9. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BG715429.1, BP317921.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCAAGT ACCTGGCCCA GATCATTGTG ATGGGCGTGC AGGTGGTGGG CAGGGCCTTT 
GCACGGGCCT TGCGGCAGGA GTTTGCAGCC AGCCGGGCCG CAGCTGATGC CCGAGGACGC 
GCTGGACACC GGTCTGCAGC CGCTTCCAAC CTCTCCGGCC TCAGCCTCCA GGAGGCACAG 
CAGATTCTCA ACGTGTCCAA GCTGAGCCCT GAGGAGGTCC AGAAGAACTA TGAACACTTA 
TTTAAGGTGA ATGATAAATC CGTGGGTGGC TCCTTCTACC TGCAGTCAAA GGTGGTCCGC 
GCAAAGGAGC GCCTGGATGA GGAACTCAAA ATCCAGGCCC AGGAGGACAG AGAAAAAGGG 
CAGATGCCCC ATACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057153.8
CDS	166..543
Translation	MAKYLAQIIVMGVQVVGRAFARALRQEFAASRAAADARGRAGHRSAAASNLSGLSLQEAQQILNVSKLSPEEVQKNYEHLFKVNDKSVGGSFYLQSKVVRAKERLDEELKIQAQEDREKGQMPHT

Target ORF information:

RefSeq Version	NM_016069.10
Organism	Homo sapiens(human)
Definition	Homo sapiens presequence translocase associated motor 16 (PAM16), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016069.10

ORF Insert Sequence:

ATGGCCAAGT ACCTGGCCCA GATCATTGTG ATGGGCGTGC AGGTGGTGGG CAGGGCCTTT 
GCACGGGCCT TGCGGCAGGA GTTTGCAGCC AGCCGGGCCG CAGCTGATGC CCGAGGACGC 
GCTGGACACC GGTCTGCAGC CGCTTCCAAC CTCTCCGGCC TCAGCCTCCA GGAGGCACAG 
CAGATTCTCA ACGTGTCCAA GCTGAGCCCT GAGGAGGTCC AGAAGAACTA TGAACACTTA 
TTTAAGGTGA ATGATAAATC CGTGGGTGGC TCCTTCTACC TGCAGTCAAA GGTGGTCCGC 
GCAAAGGAGC GCCTGGATGA GGAACTCAAA ATCCAGGCCC AGGAGGACAG AGAAAAAGGG 
CAGATGCCCC ATACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAM16 ( NM_016069.11 ) cDNA ORF clone, Homo sapiens(human) -> NP_057153.8 Homo sapiens presequence translocase associated motor 16 (PAM16), mRNA.

Price & Availability↑

CloneID	OHu09343
Clone ID Related Accession (Same CDS sequence)	NM_016069.9 , NM_016069.10 , NM_016069.11
Accession Version	NM_016069.11 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1566316800000
Organism	Homo sapiens(human)
Product	mitochondrial import inner membrane translocase subunit TIM16
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG715429.1 and AK026514.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_016069.10. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BG715429.1, BP317921.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000318059.8/ ENSP00000315693.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCAAGT ACCTGGCCCA GATCATTGTG ATGGGCGTGC AGGTGGTGGG CAGGGCCTTT 
GCACGGGCCT TGCGGCAGGA GTTTGCAGCC AGCCGGGCCG CAGCTGATGC CCGAGGACGC 
GCTGGACACC GGTCTGCAGC CGCTTCCAAC CTCTCCGGCC TCAGCCTCCA GGAGGCACAG 
CAGATTCTCA ACGTGTCCAA GCTGAGCCCT GAGGAGGTCC AGAAGAACTA TGAACACTTA 
TTTAAGGTGA ATGATAAATC CGTGGGTGGC TCCTTCTACC TGCAGTCAAA GGTGGTCCGC 
GCAAAGGAGC GCCTGGATGA GGAACTCAAA ATCCAGGCCC AGGAGGACAG AGAAAAAGGG 
CAGATGCCCC ATACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057153.8
CDS	88..465
Translation	MAKYLAQIIVMGVQVVGRAFARALRQEFAASRAAADARGRAGHRSAAASNLSGLSLQEAQQILNVSKLSPEEVQKNYEHLFKVNDKSVGGSFYLQSKVVRAKERLDEELKIQAQEDREKGQMPHT

Target ORF information:

RefSeq Version	NM_016069.11
Organism	Homo sapiens(human)
Definition	Homo sapiens presequence translocase associated motor 16 (PAM16), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016069.11

ORF Insert Sequence:

ATGGCCAAGT ACCTGGCCCA GATCATTGTG ATGGGCGTGC AGGTGGTGGG CAGGGCCTTT 
GCACGGGCCT TGCGGCAGGA GTTTGCAGCC AGCCGGGCCG CAGCTGATGC CCGAGGACGC 
GCTGGACACC GGTCTGCAGC CGCTTCCAAC CTCTCCGGCC TCAGCCTCCA GGAGGCACAG 
CAGATTCTCA ACGTGTCCAA GCTGAGCCCT GAGGAGGTCC AGAAGAACTA TGAACACTTA 
TTTAAGGTGA ATGATAAATC CGTGGGTGGC TCCTTCTACC TGCAGTCAAA GGTGGTCCGC 
GCAAAGGAGC GCCTGGATGA GGAACTCAAA ATCCAGGCCC AGGAGGACAG AGAAAAAGGG 
CAGATGCCCC ATACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Magmas inhibition as a potential treatment strategy in malignant glioma.
Journal of neuro-oncology141(2)267-276(2019 Jan)
Di K,Lomeli N,Bota DA,Das BC

A novel homozygous PAM16 mutation in a patient with a milder phenotype and longer survival.
American journal of medical genetics. Part A170(9)2436-9(2016 09)
Moosa S,Fano V,Obregon MG,Altmüller J,Thiele H,Nürnberg P,Nishimura G,Wollnik B

Functional Diversity of Human Mitochondrial J-proteins Is Independent of Their Association with the Inner Membrane Presequence Translocase.
The Journal of biological chemistry291(33)17345-59(2016 08)
Sinha D,Srivastava S,D'Silva P

Magmas functions as a ROS regulator and provides cytoprotection against oxidative stress-mediated damages.
Cell death & disease5e1394(2014 Aug)
Srivastava S,Sinha D,Saha PP,Marthala H,D'Silva P

The impairment of MAGMAS function in human is responsible for a severe skeletal dysplasia.
PLoS genetics10(5)e1004311(2014 May)
Mehawej C,Delahodde A,Legeai-Mallet L,Delague V,Kaci N,Desvignes JP,Kibar Z,Capo-Chichi JM,Chouery E,Munnich A,Cormier-Daire V,Mégarbané A

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PAM16 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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