MDH2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MDH2
Entrez Gene ID	4191
Full Name	malate dehydrogenase 2
Synonyms	EIEE51,M-MDH,MDH,MGC:3559,MOR1
General protein information	Preferred Names malate dehydrogenase 2 Names malate dehydrogenase, mitochondrial malate dehydrogenase 2, NAD (mitochondrial) testicular tissue protein Li 120
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q11.23
Summary	Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013].
Disorder MIM:	154100
Disorder Html:	Epileptic encephalopathy, early infantile, 51, 617339 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_005918.3	NP_005909.2	malate dehydrogenase, mitochondrial isoform 1 precursor
NM_001282403.1	NP_001269332.1	malate dehydrogenase, mitochondrial isoform 2 precursor
NM_001282403.2	NP_001269332.1	malate dehydrogenase, mitochondrial isoform 2 precursor
NM_001282404.1	NP_001269333.1	malate dehydrogenase, mitochondrial isoform 3
NM_001282404.2	NP_001269333.1	malate dehydrogenase, mitochondrial isoform 3
NM_005918.4	NP_005909.2	malate dehydrogenase, mitochondrial isoform 1 precursor

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os05g0574400	NP_001056389.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001903	XP_321163.4
Magnaporthe oryzae (rice blast fungus)	MGG_09367	XP_003720254.1
Oryza sativa (rice)	Os01g0649100	NP_001043717.1
Homo sapiens (human)	MDH2	NP_005909.2
Pan troglodytes (chimpanzee)	MDH2	XP_001156205.1
Caenorhabditis elegans (roundworm)	mdh-2	NP_498457.1
Arabidopsis thaliana (thale cress)	mMDH1	NP_564625.1
Kluyveromyces lactis	KLLA0F25960g	XP_456236.1
Macaca mulatta (Rhesus monkey)	LOC719983	XP_001114888.1
Mus musculus (house mouse)	Mdh2	NP_032643.2
Gallus gallus (chicken)	MDH2	XP_415765.1
Drosophila melanogaster (fruit fly)	Mdh2	NP_650696.1
Arabidopsis thaliana (thale cress)	mMDH2	NP_188120.1
Xenopus tropicalis (tropical clawed frog)	mdh2	NP_001011412.1
Canis lupus familiaris (dog)	LOC102152275	XP_005634130.1
Schizosaccharomyces pombe (fission yeast)	SPCC306.08c	NP_587816.1
Danio rerio (zebrafish)	mdh2	NP_998296.1
Canis lupus familiaris (dog)	MDH2	XP_849944.1
Rattus norvegicus (Norway rat)	Mdh2	NP_112413.2
Eremothecium gossypii	AGOS_ADR152C	NP_984248.1
Neurospora crassa	NCU04899	XP_958408.1
Saccharomyces cerevisiae (baker's yeast)	MDH1	NP_012838.1

Gene Ontology
Bibliography

Related articles in PubMed

Role of MDH2 pathogenic variant in pheochromocytoma and paraganglioma patients.
Calsina B, Currás-Freixes M, Buffet A, Pons T, Contreras L, Letón R, Comino-Méndez I, Remacha L, Calatayud M, Obispo B, Martin A, Cohen R, Richter S, Balmaña J, Korpershoek E, Rapizzi E, Deutschbein T, Vroonen L, Favier J, de Krijger RR, Fassnacht M, Beuschlein F, Timmers HJ, Eisenhofer G, Mannelli M, Pacak K, Satrústegui J, Rodríguez-Antona C, Amar L, Cascón A, Dölker N, Gimenez-Roqueplo AP, Robledo M
Genetics in medicine : official journal of the American College of Medical Genetics20(12)1652-1662(2018 12)

MDH2 is an RNA binding protein involved in downregulation of sodium channel Scn1a expression under seizure condition.
Chen YH, Liu SJ, Gao MM, Zeng T, Lin GW, Tan NN, Tang HL, Lu P, Su T, Sun WW, Xie LC, Yi YH, Long YS
Biochimica et biophysica acta. Molecular basis of disease1863(6)1492-1499(2017 06)

Mutations in MDH2, Encoding a Krebs Cycle Enzyme, Cause Early-Onset Severe Encephalopathy.
Ait-El-Mkadem S, Dayem-Quere M, Gusic M, Chaussenot A, Bannwarth S, François B, Genin EC, Fragaki K, Volker-Touw CLM, Vasnier C, Serre V, van Gassen KLI, Lespinasse F, Richter S, Eisenhofer G, Rouzier C, Mochel F, De Saint-Martin A, Abi Warde MT, de Sain-van der Velde MGM, Jans JJM, Amiel J, Avsec Z, Mertes C, Haack TB, Strom T, Meitinger T, Bonnen PE, Taylor RW, Gagneur J, van Hasselt PM, Rötig A, Delahodde A, Prokisch H, Fuchs SA, Paquis-Flucklinger V
American journal of human genetics100(1)151-159(2017 Jan)

Mitochondrial proteomics with siRNA knockdown to reveal ACAT1 and MDH2 in the development of doxorubicin-resistant uterine cancer.
Lo YW, Lin ST, Chang SJ, Chan CH, Lyu KW, Chang JF, May EW, Lin DY, Chou HC, Chan HL
Journal of cellular and molecular medicine19(4)744-59(2015 Apr)

Whole-exome sequencing identifies MDH2 as a new familial paraganglioma gene.
Cascón A, Comino-Méndez I, Currás-Freixes M, de Cubas AA, Contreras L, Richter S, Peitzsch M, Mancikova V, Inglada-Pérez L, Pérez-Barrios A, Calatayud M, Azriel S, Villar-Vicente R, Aller J, Setién F, Moran S, Garcia JF, Río-Machín A, Letón R, Gómez-Graña Á, Apellániz-Ruiz M, Roncador G, Esteller M, Rodríguez-Antona C, Satrústegui J, Eisenhofer G, Urioste M, Robledo M
Journal of the National Cancer Institute107(5)(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MDH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MDH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10150	NM_001282403.2 Latest version!	Homo sapiens malate dehydrogenase 2 (MDH2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu09726	NM_001282404.2 Latest version!	Homo sapiens malate dehydrogenase 2 (MDH2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$139.30 $199.00
OHu14773	NM_005918.4 Latest version!	Homo sapiens malate dehydrogenase 2 (MDH2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
View Old Accession Versions >>
OHu10150	NM_001282403.1	Homo sapiens malate dehydrogenase 2 (MDH2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu09726	NM_001282404.1	Homo sapiens malate dehydrogenase 2 (MDH2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$139.30 $199.00
OHu14773	NM_005918.3	Homo sapiens malate dehydrogenase 2 (MDH2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MDH2 ( NM_005918.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005909.2 Homo sapiens malate dehydrogenase 2 (MDH2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14773
Clone ID Related Accession (Same CDS sequence)	NM_005918.3 , NM_005918.4
Accession Version	NM_005918.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Homo sapiens(human)
Product	malate dehydrogenase, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP382246.1, DC354622.1, AF047470.1, AK095803.1 and AA214555.1. This sequence is a reference standard in the RefSeqGene project. On Sep 5, 2013 this sequence version replaced NM_005918.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.11568.1, SRR1660809.253659.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTCTCCG CCCTCGCCCG GCCTGCCAGC GCTGCTCTCC GCCGCAGCTT CAGCACCTCG 
GCCCAGAACA ATGCTAAAGT AGCTGTGCTA GGGGCCTCTG GAGGCATCGG GCAGCCACTT 
TCACTTCTCC TGAAGAACAG CCCCTTGGTG AGCCGCCTGA CCCTCTATGA TATCGCGCAC 
ACACCCGGAG TGGCCGCAGA TCTGAGCCAC ATCGAGACCA AAGCCGCTGT GAAAGGCTAC 
CTCGGACCTG AACAGCTGCC TGACTGCCTG AAAGGTTGTG ATGTGGTAGT TATTCCGGCT 
GGAGTCCCCA GAAAGCCAGG CATGACCCGG GACGACCTGT TCAACACCAA TGCCACGATT 
GTGGCCACCC TGACCGCTGC CTGTGCCCAG CACTGCCCGG AAGCCATGAT CTGCGTCATT 
GCCAATCCGG TTAATTCCAC CATCCCCATC ACAGCAGAAG TTTTCAAGAA GCATGGAGTG 
TACAACCCCA ACAAAATCTT CGGCGTGACG ACCCTGGACA TCGTCAGAGC CAACACCTTT 
GTTGCAGAGC TGAAGGGTTT GGATCCAGCT CGAGTCAACG TCCCTGTCAT TGGTGGCCAT 
GCTGGGAAGA CCATCATCCC CCTGATCTCT CAGTGCACCC CCAAGGTGGA CTTTCCCCAG 
GACCAGCTGA CAGCACTCAC TGGGCGGATC CAGGAGGCCG GCACGGAGGT GGTCAAGGCT 
AAAGCCGGAG CAGGCTCTGC CACCCTCTCC ATGGCGTATG CCGGCGCCCG CTTTGTCTTC 
TCCCTTGTGG ATGCAATGAA TGGAAAGGAA GGTGTTGTGG AATGTTCCTT CGTTAAGTCA 
CAGGAAACGG AATGTACCTA CTTCTCCACA CCGCTGCTGC TTGGGAAAAA GGGCATCGAG 
AAGAACCTGG GCATCGGCAA AGTCTCCTCT TTTGAGGAGA AGATGATCTC GGATGCCATC 
CCCGAGCTGA AGGCCTCCAT CAAGAAGGGG GAAGATTTCG TGAAGACCCT GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005909.2
CDS	143..1159
Translation	MLSALARPASAALRRSFSTSAQNNAKVAVLGASGGIGQPLSLLLKNSPLVSRLTLYDIAHTPGVAADLSHIETKAAVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHCPEAMICVIANPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGKTIIPLISQCTPKVDFPQDQLTALTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECSFVKSQETECTYFSTPLLLGKKGIEKNLGIGKVSSFEEKMISDAIPELKASIKKGEDFVKTLK

Target ORF information:

RefSeq Version	NM_005918.3
Organism	Homo sapiens(human)
Definition	Homo sapiens malate dehydrogenase 2 (MDH2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005918.3

ORF Insert Sequence:

ATGCTCTCCG CCCTCGCCCG GCCTGCCAGC GCTGCTCTCC GCCGCAGCTT CAGCACCTCG 
GCCCAGAACA ATGCTAAAGT AGCTGTGCTA GGGGCCTCTG GAGGCATCGG GCAGCCACTT 
TCACTTCTCC TGAAGAACAG CCCCTTGGTG AGCCGCCTGA CCCTCTATGA TATCGCGCAC 
ACACCCGGAG TGGCCGCAGA TCTGAGCCAC ATCGAGACCA AAGCCGCTGT GAAAGGCTAC 
CTCGGACCTG AACAGCTGCC TGACTGCCTG AAAGGTTGTG ATGTGGTAGT TATTCCGGCT 
GGAGTCCCCA GAAAGCCAGG CATGACCCGG GACGACCTGT TCAACACCAA TGCCACGATT 
GTGGCCACCC TGACCGCTGC CTGTGCCCAG CACTGCCCGG AAGCCATGAT CTGCGTCATT 
GCCAATCCGG TTAATTCCAC CATCCCCATC ACAGCAGAAG TTTTCAAGAA GCATGGAGTG 
TACAACCCCA ACAAAATCTT CGGCGTGACG ACCCTGGACA TCGTCAGAGC CAACACCTTT 
GTTGCAGAGC TGAAGGGTTT GGATCCAGCT CGAGTCAACG TCCCTGTCAT TGGTGGCCAT 
GCTGGGAAGA CCATCATCCC CCTGATCTCT CAGTGCACCC CCAAGGTGGA CTTTCCCCAG 
GACCAGCTGA CAGCACTCAC TGGGCGGATC CAGGAGGCCG GCACGGAGGT GGTCAAGGCT 
AAAGCCGGAG CAGGCTCTGC CACCCTCTCC ATGGCGTATG CCGGCGCCCG CTTTGTCTTC 
TCCCTTGTGG ATGCAATGAA TGGAAAGGAA GGTGTTGTGG AATGTTCCTT CGTTAAGTCA 
CAGGAAACGG AATGTACCTA CTTCTCCACA CCGCTGCTGC TTGGGAAAAA GGGCATCGAG 
AAGAACCTGG GCATCGGCAA AGTCTCCTCT TTTGAGGAGA AGATGATCTC GGATGCCATC 
CCCGAGCTGA AGGCCTCCAT CAAGAAGGGG GAAGATTTCG TGAAGACCCT GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDH2 ( NM_001282403.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269332.1 Homo sapiens malate dehydrogenase 2 (MDH2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10150
Clone ID Related Accession (Same CDS sequence)	NM_001282403.1 , NM_001282403.2
Accession Version	NM_001282403.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550851200000
Organism	Homo sapiens(human)
Product	malate dehydrogenase, mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP382246.1, DC354622.1, AK293460.1, AK290779.1, AK095803.1 and AA214555.1. On or before Sep 5, 2013 this sequence version replaced XM_005250351.1, XM_005277583.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.14514.1, SRR1803614.128182.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTCTCCG CCCTCGCCCG GCCTGCCAGC GCTGCTCTCC GCCGCAGCTT CAGCACCTCG 
GCCCAGAACA ATGCTAAAGT AGCTGTGCTA GGGGCCTCTG GAGGCATCGG GCAGCCACTT 
TCACTTCTCC TGAAGAACAG CCCCTTGGTG AGCCGCCTGA CCCTCTATGA TATCGCGCAC 
ACACCCGGAG TGGCCGCAGA TCTGAGCCAC ATCGAGACCA AAGCCGCTGT GAAAGGCTAC 
CTCGGACCTG AACAGCTGCC TGACTGCCTG AAAGGTTGTG ATGTGGTAGT TATTCCGGCT 
GGAGTCCCCA GAAAGCCAGG CATGACCCGG GACGACCTGT TCAACACCAA TGCCACGATT 
GTGGCCACCC TGACCGCTGC CTGTGCCCAG CACTGCCCGG AAGCCATGAT CTGCGTCATT 
GCCAATCCGG GTTTGGATCC AGCTCGAGTC AACGTCCCTG TCATTGGTGG CCATGCTGGG 
AAGACCATCA TCCCCCTGAT CTCTCAGTGC ACCCCCAAGG TGGACTTTCC CCAGGACCAG 
CTGACAGCAC TCACTGGGCG GATCCAGGAG GCCGGCACGG AGGTGGTCAA GGCTAAAGCC 
GGAGCAGGCT CTGCCACCCT CTCCATGGCG TATGCCGGCG CCCGCTTTGT CTTCTCCCTT 
GTGGATGCAA TGAATGGAAA GGAAGGTGTT GTGGAATGTT CCTTCGTTAA GTCACAGGAA 
ACGGAATGTA CCTACTTCTC CACACCGCTG CTGCTTGGGA AAAAGGGCAT CGAGAAGAAC 
CTGGGCATCG GCAAAGTCTC CTCTTTTGAG GAGAAGATGA TCTCGGATGC CATCCCCGAG 
CTGAAGGCCT CCATCAAGAA GGGGGAAGAT TTCGTGAAGA CCCTGAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269332.1
CDS	143..1033
Translation	MLSALARPASAALRRSFSTSAQNNAKVAVLGASGGIGQPLSLLLKNSPLVSRLTLYDIAHTPGVAADLSHIETKAAVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHCPEAMICVIANPGLDPARVNVPVIGGHAGKTIIPLISQCTPKVDFPQDQLTALTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECSFVKSQETECTYFSTPLLLGKKGIEKNLGIGKVSSFEEKMISDAIPELKASIKKGEDFVKTLK

Target ORF information:

RefSeq Version	NM_001282403.1
Organism	Homo sapiens(human)
Definition	Homo sapiens malate dehydrogenase 2 (MDH2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282403.1

ORF Insert Sequence:

ATGCTCTCCG CCCTCGCCCG GCCTGCCAGC GCTGCTCTCC GCCGCAGCTT CAGCACCTCG 
GCCCAGAACA ATGCTAAAGT AGCTGTGCTA GGGGCCTCTG GAGGCATCGG GCAGCCACTT 
TCACTTCTCC TGAAGAACAG CCCCTTGGTG AGCCGCCTGA CCCTCTATGA TATCGCGCAC 
ACACCCGGAG TGGCCGCAGA TCTGAGCCAC ATCGAGACCA AAGCCGCTGT GAAAGGCTAC 
CTCGGACCTG AACAGCTGCC TGACTGCCTG AAAGGTTGTG ATGTGGTAGT TATTCCGGCT 
GGAGTCCCCA GAAAGCCAGG CATGACCCGG GACGACCTGT TCAACACCAA TGCCACGATT 
GTGGCCACCC TGACCGCTGC CTGTGCCCAG CACTGCCCGG AAGCCATGAT CTGCGTCATT 
GCCAATCCGG GTTTGGATCC AGCTCGAGTC AACGTCCCTG TCATTGGTGG CCATGCTGGG 
AAGACCATCA TCCCCCTGAT CTCTCAGTGC ACCCCCAAGG TGGACTTTCC CCAGGACCAG 
CTGACAGCAC TCACTGGGCG GATCCAGGAG GCCGGCACGG AGGTGGTCAA GGCTAAAGCC 
GGAGCAGGCT CTGCCACCCT CTCCATGGCG TATGCCGGCG CCCGCTTTGT CTTCTCCCTT 
GTGGATGCAA TGAATGGAAA GGAAGGTGTT GTGGAATGTT CCTTCGTTAA GTCACAGGAA 
ACGGAATGTA CCTACTTCTC CACACCGCTG CTGCTTGGGA AAAAGGGCAT CGAGAAGAAC 
CTGGGCATCG GCAAAGTCTC CTCTTTTGAG GAGAAGATGA TCTCGGATGC CATCCCCGAG 
CTGAAGGCCT CCATCAAGAA GGGGGAAGAT TTCGTGAAGA CCCTGAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDH2 ( NM_001282403.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269332.1 Homo sapiens malate dehydrogenase 2 (MDH2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10150
Clone ID Related Accession (Same CDS sequence)	NM_001282403.1 , NM_001282403.2
Accession Version	NM_001282403.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569600000000
Organism	Homo sapiens(human)
Product	malate dehydrogenase, mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006330.5 and AC005077.5. On Jun 1, 2019 this sequence version replaced NM_001282403.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.14514.1, SRR1803614.128182.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTCTCCG CCCTCGCCCG GCCTGCCAGC GCTGCTCTCC GCCGCAGCTT CAGCACCTCG 
GCCCAGAACA ATGCTAAAGT AGCTGTGCTA GGGGCCTCTG GAGGCATCGG GCAGCCACTT 
TCACTTCTCC TGAAGAACAG CCCCTTGGTG AGCCGCCTGA CCCTCTATGA TATCGCGCAC 
ACACCCGGAG TGGCCGCAGA TCTGAGCCAC ATCGAGACCA AAGCCGCTGT GAAAGGCTAC 
CTCGGACCTG AACAGCTGCC TGACTGCCTG AAAGGTTGTG ATGTGGTAGT TATTCCGGCT 
GGAGTCCCCA GAAAGCCAGG CATGACCCGG GACGACCTGT TCAACACCAA TGCCACGATT 
GTGGCCACCC TGACCGCTGC CTGTGCCCAG CACTGCCCGG AAGCCATGAT CTGCGTCATT 
GCCAATCCGG GTTTGGATCC AGCTCGAGTC AACGTCCCTG TCATTGGTGG CCATGCTGGG 
AAGACCATCA TCCCCCTGAT CTCTCAGTGC ACCCCCAAGG TGGACTTTCC CCAGGACCAG 
CTGACAGCAC TCACTGGGCG GATCCAGGAG GCCGGCACGG AGGTGGTCAA GGCTAAAGCC 
GGAGCAGGCT CTGCCACCCT CTCCATGGCG TATGCCGGCG CCCGCTTTGT CTTCTCCCTT 
GTGGATGCAA TGAATGGAAA GGAAGGTGTT GTGGAATGTT CCTTCGTTAA GTCACAGGAA 
ACGGAATGTA CCTACTTCTC CACACCGCTG CTGCTTGGGA AAAAGGGCAT CGAGAAGAAC 
CTGGGCATCG GCAAAGTCTC CTCTTTTGAG GAGAAGATGA TCTCGGATGC CATCCCCGAG 
CTGAAGGCCT CCATCAAGAA GGGGGAAGAT TTCGTGAAGA CCCTGAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269332.1
CDS	56..946
Translation	MLSALARPASAALRRSFSTSAQNNAKVAVLGASGGIGQPLSLLLKNSPLVSRLTLYDIAHTPGVAADLSHIETKAAVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHCPEAMICVIANPGLDPARVNVPVIGGHAGKTIIPLISQCTPKVDFPQDQLTALTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECSFVKSQETECTYFSTPLLLGKKGIEKNLGIGKVSSFEEKMISDAIPELKASIKKGEDFVKTLK

Target ORF information:

RefSeq Version	NM_001282403.2
Organism	Homo sapiens(human)
Definition	Homo sapiens malate dehydrogenase 2 (MDH2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282403.2

ORF Insert Sequence:

ATGCTCTCCG CCCTCGCCCG GCCTGCCAGC GCTGCTCTCC GCCGCAGCTT CAGCACCTCG 
GCCCAGAACA ATGCTAAAGT AGCTGTGCTA GGGGCCTCTG GAGGCATCGG GCAGCCACTT 
TCACTTCTCC TGAAGAACAG CCCCTTGGTG AGCCGCCTGA CCCTCTATGA TATCGCGCAC 
ACACCCGGAG TGGCCGCAGA TCTGAGCCAC ATCGAGACCA AAGCCGCTGT GAAAGGCTAC 
CTCGGACCTG AACAGCTGCC TGACTGCCTG AAAGGTTGTG ATGTGGTAGT TATTCCGGCT 
GGAGTCCCCA GAAAGCCAGG CATGACCCGG GACGACCTGT TCAACACCAA TGCCACGATT 
GTGGCCACCC TGACCGCTGC CTGTGCCCAG CACTGCCCGG AAGCCATGAT CTGCGTCATT 
GCCAATCCGG GTTTGGATCC AGCTCGAGTC AACGTCCCTG TCATTGGTGG CCATGCTGGG 
AAGACCATCA TCCCCCTGAT CTCTCAGTGC ACCCCCAAGG TGGACTTTCC CCAGGACCAG 
CTGACAGCAC TCACTGGGCG GATCCAGGAG GCCGGCACGG AGGTGGTCAA GGCTAAAGCC 
GGAGCAGGCT CTGCCACCCT CTCCATGGCG TATGCCGGCG CCCGCTTTGT CTTCTCCCTT 
GTGGATGCAA TGAATGGAAA GGAAGGTGTT GTGGAATGTT CCTTCGTTAA GTCACAGGAA 
ACGGAATGTA CCTACTTCTC CACACCGCTG CTGCTTGGGA AAAAGGGCAT CGAGAAGAAC 
CTGGGCATCG GCAAAGTCTC CTCTTTTGAG GAGAAGATGA TCTCGGATGC CATCCCCGAG 
CTGAAGGCCT CCATCAAGAA GGGGGAAGAT TTCGTGAAGA CCCTGAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDH2 ( NM_001282404.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269333.1 Homo sapiens malate dehydrogenase 2 (MDH2), transcript variant 3, mRNA.

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CloneID	OHu09726
Clone ID Related Accession (Same CDS sequence)	NM_001282404.1 , NM_001282404.2
Accession Version	NM_001282404.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550851200000
Organism	Homo sapiens(human)
Product	malate dehydrogenase, mitochondrial isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP382246.1, DC354622.1, AK095803.1, AK290779.1 and AA214555.1. Transcript Variant: This variant (3) lacks an alternate exon compared to variant 1, that causes translation to begin at a downstream AUG. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK095803.1, SRR1660807.59786.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACCCGGG ACGACCTGTT CAACACCAAT GCCACGATTG TGGCCACCCT GACCGCTGCC 
TGTGCCCAGC ACTGCCCGGA AGCCATGATC TGCGTCATTG CCAATCCGGT TAATTCCACC 
ATCCCCATCA CAGCAGAAGT TTTCAAGAAG CATGGAGTGT ACAACCCCAA CAAAATCTTC 
GGCGTGACGA CCCTGGACAT CGTCAGAGCC AACACCTTTG TTGCAGAGCT GAAGGGTTTG 
GATCCAGCTC GAGTCAACGT CCCTGTCATT GGTGGCCATG CTGGGAAGAC CATCATCCCC 
CTGATCTCTC AGTGCACCCC CAAGGTGGAC TTTCCCCAGG ACCAGCTGAC AGCACTCACT 
GGGCGGATCC AGGAGGCCGG CACGGAGGTG GTCAAGGCTA AAGCCGGAGC AGGCTCTGCC 
ACCCTCTCCA TGGCGTATGC CGGCGCCCGC TTTGTCTTCT CCCTTGTGGA TGCAATGAAT 
GGAAAGGAAG GTGTTGTGGA ATGTTCCTTC GTTAAGTCAC AGGAAACGGA ATGTACCTAC 
TTCTCCACAC CGCTGCTGCT TGGGAAAAAG GGCATCGAGA AGAACCTGGG CATCGGCAAA 
GTCTCCTCTT TTGAGGAGAA GATGATCTCG GATGCCATCC CCGAGCTGAA GGCCTCCATC 
AAGAAGGGGG AAGATTTCGT GAAGACCCTG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269333.1
CDS	295..990
Translation	MTRDDLFNTNATIVATLTAACAQHCPEAMICVIANPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGKTIIPLISQCTPKVDFPQDQLTALTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECSFVKSQETECTYFSTPLLLGKKGIEKNLGIGKVSSFEEKMISDAIPELKASIKKGEDFVKTLK

Target ORF information:

RefSeq Version	NM_001282404.1
Organism	Homo sapiens(human)
Definition	Homo sapiens malate dehydrogenase 2 (MDH2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282404.1

ORF Insert Sequence:

ATGACCCGGG ACGACCTGTT CAACACCAAT GCCACGATTG TGGCCACCCT GACCGCTGCC 
TGTGCCCAGC ACTGCCCGGA AGCCATGATC TGCGTCATTG CCAATCCGGT TAATTCCACC 
ATCCCCATCA CAGCAGAAGT TTTCAAGAAG CATGGAGTGT ACAACCCCAA CAAAATCTTC 
GGCGTGACGA CCCTGGACAT CGTCAGAGCC AACACCTTTG TTGCAGAGCT GAAGGGTTTG 
GATCCAGCTC GAGTCAACGT CCCTGTCATT GGTGGCCATG CTGGGAAGAC CATCATCCCC 
CTGATCTCTC AGTGCACCCC CAAGGTGGAC TTTCCCCAGG ACCAGCTGAC AGCACTCACT 
GGGCGGATCC AGGAGGCCGG CACGGAGGTG GTCAAGGCTA AAGCCGGAGC AGGCTCTGCC 
ACCCTCTCCA TGGCGTATGC CGGCGCCCGC TTTGTCTTCT CCCTTGTGGA TGCAATGAAT 
GGAAAGGAAG GTGTTGTGGA ATGTTCCTTC GTTAAGTCAC AGGAAACGGA ATGTACCTAC 
TTCTCCACAC CGCTGCTGCT TGGGAAAAAG GGCATCGAGA AGAACCTGGG CATCGGCAAA 
GTCTCCTCTT TTGAGGAGAA GATGATCTCG GATGCCATCC CCGAGCTGAA GGCCTCCATC 
AAGAAGGGGG AAGATTTCGT GAAGACCCTG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDH2 ( NM_001282404.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269333.1 Homo sapiens malate dehydrogenase 2 (MDH2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu09726
Clone ID Related Accession (Same CDS sequence)	NM_001282404.1 , NM_001282404.2
Accession Version	NM_001282404.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569513600000
Organism	Homo sapiens(human)
Product	malate dehydrogenase, mitochondrial isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006330.5 and AC005077.5. On Jun 1, 2019 this sequence version replaced NM_001282404.1. Transcript Variant: This variant (3) lacks an alternate exon compared to variant 1, that causes translation to begin at a downstream AUG. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.256334.1, SRR1803612.61949.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACCCGGG ACGACCTGTT CAACACCAAT GCCACGATTG TGGCCACCCT GACCGCTGCC 
TGTGCCCAGC ACTGCCCGGA AGCCATGATC TGCGTCATTG CCAATCCGGT TAATTCCACC 
ATCCCCATCA CAGCAGAAGT TTTCAAGAAG CATGGAGTGT ACAACCCCAA CAAAATCTTC 
GGCGTGACGA CCCTGGACAT CGTCAGAGCC AACACCTTTG TTGCAGAGCT GAAGGGTTTG 
GATCCAGCTC GAGTCAACGT CCCTGTCATT GGTGGCCATG CTGGGAAGAC CATCATCCCC 
CTGATCTCTC AGTGCACCCC CAAGGTGGAC TTTCCCCAGG ACCAGCTGAC AGCACTCACT 
GGGCGGATCC AGGAGGCCGG CACGGAGGTG GTCAAGGCTA AAGCCGGAGC AGGCTCTGCC 
ACCCTCTCCA TGGCGTATGC CGGCGCCCGC TTTGTCTTCT CCCTTGTGGA TGCAATGAAT 
GGAAAGGAAG GTGTTGTGGA ATGTTCCTTC GTTAAGTCAC AGGAAACGGA ATGTACCTAC 
TTCTCCACAC CGCTGCTGCT TGGGAAAAAG GGCATCGAGA AGAACCTGGG CATCGGCAAA 
GTCTCCTCTT TTGAGGAGAA GATGATCTCG GATGCCATCC CCGAGCTGAA GGCCTCCATC 
AAGAAGGGGG AAGATTTCGT GAAGACCCTG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269333.1
CDS	208..903
Translation	MTRDDLFNTNATIVATLTAACAQHCPEAMICVIANPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGKTIIPLISQCTPKVDFPQDQLTALTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECSFVKSQETECTYFSTPLLLGKKGIEKNLGIGKVSSFEEKMISDAIPELKASIKKGEDFVKTLK

Target ORF information:

RefSeq Version	NM_001282404.2
Organism	Homo sapiens(human)
Definition	Homo sapiens malate dehydrogenase 2 (MDH2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282404.2

ORF Insert Sequence:

ATGACCCGGG ACGACCTGTT CAACACCAAT GCCACGATTG TGGCCACCCT GACCGCTGCC 
TGTGCCCAGC ACTGCCCGGA AGCCATGATC TGCGTCATTG CCAATCCGGT TAATTCCACC 
ATCCCCATCA CAGCAGAAGT TTTCAAGAAG CATGGAGTGT ACAACCCCAA CAAAATCTTC 
GGCGTGACGA CCCTGGACAT CGTCAGAGCC AACACCTTTG TTGCAGAGCT GAAGGGTTTG 
GATCCAGCTC GAGTCAACGT CCCTGTCATT GGTGGCCATG CTGGGAAGAC CATCATCCCC 
CTGATCTCTC AGTGCACCCC CAAGGTGGAC TTTCCCCAGG ACCAGCTGAC AGCACTCACT 
GGGCGGATCC AGGAGGCCGG CACGGAGGTG GTCAAGGCTA AAGCCGGAGC AGGCTCTGCC 
ACCCTCTCCA TGGCGTATGC CGGCGCCCGC TTTGTCTTCT CCCTTGTGGA TGCAATGAAT 
GGAAAGGAAG GTGTTGTGGA ATGTTCCTTC GTTAAGTCAC AGGAAACGGA ATGTACCTAC 
TTCTCCACAC CGCTGCTGCT TGGGAAAAAG GGCATCGAGA AGAACCTGGG CATCGGCAAA 
GTCTCCTCTT TTGAGGAGAA GATGATCTCG GATGCCATCC CCGAGCTGAA GGCCTCCATC 
AAGAAGGGGG AAGATTTCGT GAAGACCCTG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDH2 ( NM_005918.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005909.2 Homo sapiens malate dehydrogenase 2 (MDH2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14773
Clone ID Related Accession (Same CDS sequence)	NM_005918.3 , NM_005918.4
Accession Version	NM_005918.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Homo sapiens(human)
Product	malate dehydrogenase, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006330.5 and AC005077.5. On Nov 23, 2018 this sequence version replaced NM_005918.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.11568.1, SRR3476690.978959.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000315758.10/ ENSP00000327070.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTCTCCG CCCTCGCCCG GCCTGCCAGC GCTGCTCTCC GCCGCAGCTT CAGCACCTCG 
GCCCAGAACA ATGCTAAAGT AGCTGTGCTA GGGGCCTCTG GAGGCATCGG GCAGCCACTT 
TCACTTCTCC TGAAGAACAG CCCCTTGGTG AGCCGCCTGA CCCTCTATGA TATCGCGCAC 
ACACCCGGAG TGGCCGCAGA TCTGAGCCAC ATCGAGACCA AAGCCGCTGT GAAAGGCTAC 
CTCGGACCTG AACAGCTGCC TGACTGCCTG AAAGGTTGTG ATGTGGTAGT TATTCCGGCT 
GGAGTCCCCA GAAAGCCAGG CATGACCCGG GACGACCTGT TCAACACCAA TGCCACGATT 
GTGGCCACCC TGACCGCTGC CTGTGCCCAG CACTGCCCGG AAGCCATGAT CTGCGTCATT 
GCCAATCCGG TTAATTCCAC CATCCCCATC ACAGCAGAAG TTTTCAAGAA GCATGGAGTG 
TACAACCCCA ACAAAATCTT CGGCGTGACG ACCCTGGACA TCGTCAGAGC CAACACCTTT 
GTTGCAGAGC TGAAGGGTTT GGATCCAGCT CGAGTCAACG TCCCTGTCAT TGGTGGCCAT 
GCTGGGAAGA CCATCATCCC CCTGATCTCT CAGTGCACCC CCAAGGTGGA CTTTCCCCAG 
GACCAGCTGA CAGCACTCAC TGGGCGGATC CAGGAGGCCG GCACGGAGGT GGTCAAGGCT 
AAAGCCGGAG CAGGCTCTGC CACCCTCTCC ATGGCGTATG CCGGCGCCCG CTTTGTCTTC 
TCCCTTGTGG ATGCAATGAA TGGAAAGGAA GGTGTTGTGG AATGTTCCTT CGTTAAGTCA 
CAGGAAACGG AATGTACCTA CTTCTCCACA CCGCTGCTGC TTGGGAAAAA GGGCATCGAG 
AAGAACCTGG GCATCGGCAA AGTCTCCTCT TTTGAGGAGA AGATGATCTC GGATGCCATC 
CCCGAGCTGA AGGCCTCCAT CAAGAAGGGG GAAGATTTCG TGAAGACCCT GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005909.2
CDS	56..1072
Translation	MLSALARPASAALRRSFSTSAQNNAKVAVLGASGGIGQPLSLLLKNSPLVSRLTLYDIAHTPGVAADLSHIETKAAVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHCPEAMICVIANPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGKTIIPLISQCTPKVDFPQDQLTALTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECSFVKSQETECTYFSTPLLLGKKGIEKNLGIGKVSSFEEKMISDAIPELKASIKKGEDFVKTLK

Target ORF information:

RefSeq Version	NM_005918.4
Organism	Homo sapiens(human)
Definition	Homo sapiens malate dehydrogenase 2 (MDH2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005918.4

ORF Insert Sequence:

ATGCTCTCCG CCCTCGCCCG GCCTGCCAGC GCTGCTCTCC GCCGCAGCTT CAGCACCTCG 
GCCCAGAACA ATGCTAAAGT AGCTGTGCTA GGGGCCTCTG GAGGCATCGG GCAGCCACTT 
TCACTTCTCC TGAAGAACAG CCCCTTGGTG AGCCGCCTGA CCCTCTATGA TATCGCGCAC 
ACACCCGGAG TGGCCGCAGA TCTGAGCCAC ATCGAGACCA AAGCCGCTGT GAAAGGCTAC 
CTCGGACCTG AACAGCTGCC TGACTGCCTG AAAGGTTGTG ATGTGGTAGT TATTCCGGCT 
GGAGTCCCCA GAAAGCCAGG CATGACCCGG GACGACCTGT TCAACACCAA TGCCACGATT 
GTGGCCACCC TGACCGCTGC CTGTGCCCAG CACTGCCCGG AAGCCATGAT CTGCGTCATT 
GCCAATCCGG TTAATTCCAC CATCCCCATC ACAGCAGAAG TTTTCAAGAA GCATGGAGTG 
TACAACCCCA ACAAAATCTT CGGCGTGACG ACCCTGGACA TCGTCAGAGC CAACACCTTT 
GTTGCAGAGC TGAAGGGTTT GGATCCAGCT CGAGTCAACG TCCCTGTCAT TGGTGGCCAT 
GCTGGGAAGA CCATCATCCC CCTGATCTCT CAGTGCACCC CCAAGGTGGA CTTTCCCCAG 
GACCAGCTGA CAGCACTCAC TGGGCGGATC CAGGAGGCCG GCACGGAGGT GGTCAAGGCT 
AAAGCCGGAG CAGGCTCTGC CACCCTCTCC ATGGCGTATG CCGGCGCCCG CTTTGTCTTC 
TCCCTTGTGG ATGCAATGAA TGGAAAGGAA GGTGTTGTGG AATGTTCCTT CGTTAAGTCA 
CAGGAAACGG AATGTACCTA CTTCTCCACA CCGCTGCTGC TTGGGAAAAA GGGCATCGAG 
AAGAACCTGG GCATCGGCAA AGTCTCCTCT TTTGAGGAGA AGATGATCTC GGATGCCATC 
CCCGAGCTGA AGGCCTCCAT CAAGAAGGGG GAAGATTTCG TGAAGACCCT GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of MDH2 pathogenic variant in pheochromocytoma and paraganglioma patients.
Genetics in medicine : official journal of the American College of Medical Genetics20(12)1652-1662(2018 12)
Calsina B,Currás-Freixes M,Buffet A,Pons T,Contreras L,Letón R,Comino-Méndez I,Remacha L,Calatayud M,Obispo B,Martin A,Cohen R,Richter S,Balmaña J,Korpershoek E,Rapizzi E,Deutschbein T,Vroonen L,Favier J,de Krijger RR,Fassnacht M,Beuschlein F,Timmers HJ,Eisenhofer G,Mannelli M,Pacak K,Satrústegui J,Rodríguez-Antona C,Amar L,Cascón A,Dölker N,Gimenez-Roqueplo AP,Robledo M

MDH2 is an RNA binding protein involved in downregulation of sodium channel Scn1a expression under seizure condition.
Biochimica et biophysica acta. Molecular basis of disease1863(6)1492-1499(2017 06)
Chen YH,Liu SJ,Gao MM,Zeng T,Lin GW,Tan NN,Tang HL,Lu P,Su T,Sun WW,Xie LC,Yi YH,Long YS

Mutations in MDH2, Encoding a Krebs Cycle Enzyme, Cause Early-Onset Severe Encephalopathy.
American journal of human genetics100(1)151-159(2017 Jan)
Ait-El-Mkadem S,Dayem-Quere M,Gusic M,Chaussenot A,Bannwarth S,François B,Genin EC,Fragaki K,Volker-Touw CLM,Vasnier C,Serre V,van Gassen KLI,Lespinasse F,Richter S,Eisenhofer G,Rouzier C,Mochel F,De Saint-Martin A,Abi Warde MT,de Sain-van der Velde MGM,Jans JJM,Amiel J,Avsec Z,Mertes C,Haack TB,Strom T,Meitinger T,Bonnen PE,Taylor RW,Gagneur J,van Hasselt PM,Rötig A,Delahodde A,Prokisch H,Fuchs SA,Paquis-Flucklinger V

Mitochondrial proteomics with siRNA knockdown to reveal ACAT1 and MDH2 in the development of doxorubicin-resistant uterine cancer.
Journal of cellular and molecular medicine19(4)744-59(2015 Apr)
Lo YW,Lin ST,Chang SJ,Chan CH,Lyu KW,Chang JF,May EW,Lin DY,Chou HC,Chan HL

Whole-exome sequencing identifies MDH2 as a new familial paraganglioma gene.
Journal of the National Cancer Institute107(5)(2015 Mar)
Cascón A,Comino-Méndez I,Currás-Freixes M,de Cubas AA,Contreras L,Richter S,Peitzsch M,Mancikova V,Inglada-Pérez L,Pérez-Barrios A,Calatayud M,Azriel S,Villar-Vicente R,Aller J,Setién F,Moran S,Garcia JF,Río-Machín A,Letón R,Gómez-Graña Á,Apellániz-Ruiz M,Roncador G,Esteller M,Rodríguez-Antona C,Satrústegui J,Eisenhofer G,Urioste M,Robledo M

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MDH2 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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