MB cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MB
Entrez Gene ID	4151
Full Name	myoglobin
Synonyms	PVALB,myoglobgin
General protein information	Preferred Names myoglobin Names myoglobin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q12.3
Summary	This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008].
Disorder MIM:	160000

mRNA and Protein(s)

mRNA	Protein	Name
NM_203378.1	NP_976312.1	myoglobin
NM_005368.3	NP_005359.1	myoglobin
NM_203377.1	NP_976311.1	myoglobin
NM_005368.2	NP_005359.1	myoglobin
NM_001362846.1	NP_001349775.1	myoglobin
XM_005261605.2	XP_005261662.1	myoglobin isoform X1
NM_001362846.2	NP_001349775.1	myoglobin

Pathways from BioSystems

Homologies

Homo sapiens (human)	MB	NP_005359.1
Macaca mulatta (Rhesus monkey)	MB	XP_001081975.2
Pan troglodytes (chimpanzee)	MB	XP_001156646.1
Canis lupus familiaris (dog)	MB	XP_005625978.1
Mus musculus (house mouse)	Mb	NP_038621.2
Gallus gallus (chicken)	MB	NP_001161224.1
Bos taurus (cattle)	MB	NP_776306.1
Rattus norvegicus (Norway rat)	Mb	NP_067599.1
Danio rerio (zebrafish)	mb	NP_956880.1

Gene Ontology
Bibliography

Related articles in PubMed

Myoglobinopathy is an adult-onset autosomal dominant myopathy with characteristic sarcoplasmic inclusions.
Olivé M, Engvall M, Ravenscroft G, Cabrera-Serrano M, Jiao H, Bortolotti CA, Pignataro M, Lambrughi M, Jiang H, Forrest ARR, Benseny-Cases N, Hofbauer S, Obinger C, Battistuzzi G, Bellei M, Borsari M, Di Rocco G, Viola HM, Hool LC, Cladera J, Lagerstedt-Robinson K, Xiang F, Wredenberg A, Miralles F, Baiges JJ, Malfatti E, Romero NB, Streichenberger N, Vial C, Claeys KG, Straathof CSM, Goris A, Freyer C, Lammens M, Bassez G, Kere J, Clemente P, Sejersen T, Udd B, Vidal N, Ferrer I, Edström L, Wedell A, Laing NG
Nature communications10(1)1396(2019 03)

Dynamical comparison between myoglobin and hemoglobin.
Aharoni R, Tobi D
Proteins86(11)1176-1183(2018 11)

A Multi-Region Magnetoimpedance-Based Bio-Analytical System for Ultrasensitive Simultaneous Determination of Cardiac Biomarkers Myoglobin and C-Reactive Protein.
Yang Z, Wang H, Guo P, Ding Y, Lei C, Luo Y
Sensors (Basel, Switzerland)18(6)(2018 Jun)

Electroanalysis of myoglobin based on electropolymerized molecularly imprinted polymer poly-o-phenylenediamine and carbon nanotubes/screen printed electrode.
Shumyantseva VV, Bulko TV, Sigolaeva LV, Kuzikov AV, Archakov AI
Doklady. Biochemistry and biophysics468(1)213-6(2016 May)

Myoglobin A79G polymorphism association with exercise-induced skeletal muscle damage.
Cui T, Jiang MS
Genetics and molecular research : GMR15(2)(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20046	NM_001362846.2 Latest version!	Homo sapiens myoglobin (MB), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu20046	NM_005368.3 Latest version!	Homo sapiens myoglobin (MB), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu20046	NM_203378.1 Latest version!	Homo sapiens myoglobin (MB), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu20046	NM_203377.1 Latest version!	Homo sapiens myoglobin (MB), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu20046	XM_005261605.2 Latest version!	Homo sapiens myoglobin (MB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
View Old Accession Versions >>
OHu20046	NM_005368.2	Homo sapiens myoglobin (MB), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu20046	NM_001362846.1	Homo sapiens myoglobin (MB), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MB ( NM_203378.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_976312.1 Homo sapiens myoglobin (MB), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu20046
Clone ID Related Accession (Same CDS sequence)	NM_203378.1 , NM_005368.3 , NM_203377.1 , NM_005368.2 , NM_001362846.1 , XM_005261605.2 , NM_001362846.2
Accession Version	NM_203378.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Homo sapiens(human)
Product	myoglobin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ925917.1 and BC014547.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1-4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279829.2648.1, ERR279865.2556.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2154361 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCTCA GCGACGGGGA ATGGCAGTTG GTGCTGAACG TCTGGGGGAA GGTGGAGGCT 
GACATCCCAG GCCATGGGCA GGAAGTCCTC ATCAGGCTCT TTAAGGGTCA CCCAGAGACT 
CTGGAGAAGT TTGACAAGTT CAAGCACCTG AAGTCAGAGG ACGAGATGAA GGCGTCTGAG 
GACTTAAAGA AGCATGGTGC CACCGTGCTC ACCGCCCTGG GTGGCATCCT TAAGAAGAAG 
GGGCATCATG AGGCAGAGAT TAAGCCCCTG GCACAGTCGC ATGCCACCAA GCACAAGATC 
CCCGTGAAGT ACCTGGAGTT CATCTCGGAA TGCATCATCC AGGTTCTGCA GAGCAAGCAT 
CCCGGGGACT TTGGTGCTGA TGCCCAGGGG GCCATGAACA AGGCCCTGGA GCTGTTCCGG 
AAGGACATGG CCTCCAACTA CAAGGAGCTG GGCTTCCAGG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_976312.1
CDS	156..620
Translation	MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQVLQSKHPGDFGADAQGAMNKALELFRKDMASNYKELGFQG

Target ORF information:

RefSeq Version	NM_203378.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myoglobin (MB), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_203378.1

ORF Insert Sequence:

ATGGGGCTCA GCGACGGGGA ATGGCAGTTG GTGCTGAACG TCTGGGGGAA GGTGGAGGCT 
GACATCCCAG GCCATGGGCA GGAAGTCCTC ATCAGGCTCT TTAAGGGTCA CCCAGAGACT 
CTGGAGAAGT TTGACAAGTT CAAGCACCTG AAGTCAGAGG ACGAGATGAA GGCGTCTGAG 
GACTTAAAGA AGCATGGTGC CACCGTGCTC ACCGCCCTGG GTGGCATCCT TAAGAAGAAG 
GGGCATCATG AGGCAGAGAT TAAGCCCCTG GCACAGTCGC ATGCCACCAA GCACAAGATC 
CCCGTGAAGT ACCTGGAGTT CATCTCGGAA TGCATCATCC AGGTTCTGCA GAGCAAGCAT 
CCCGGGGACT TTGGTGCTGA TGCCCAGGGG GCCATGAACA AGGCCCTGGA GCTGTTCCGG 
AAGGACATGG CCTCCAACTA CAAGGAGCTG GGCTTCCAGG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MB ( NM_005368.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005359.1 Homo sapiens myoglobin (MB), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20046
Clone ID Related Accession (Same CDS sequence)	NM_203378.1 , NM_005368.3 , NM_203377.1 , NM_005368.2 , NM_001362846.1 , XM_005261605.2 , NM_001362846.2
Accession Version	NM_005368.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569340800000
Organism	Homo sapiens(human)
Product	myoglobin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU585249.1, BQ956082.1 and BC014547.1. On Nov 22, 2018 this sequence version replaced NM_005368.2. Transcript Variant: This variant (1) represents the shortest variant. Variants 1-4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014547.1, SRR5189655.156241.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2153307 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000397326.7/ ENSP00000380489.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGCTCA GCGACGGGGA ATGGCAGTTG GTGCTGAACG TCTGGGGGAA GGTGGAGGCT 
GACATCCCAG GCCATGGGCA GGAAGTCCTC ATCAGGCTCT TTAAGGGTCA CCCAGAGACT 
CTGGAGAAGT TTGACAAGTT CAAGCACCTG AAGTCAGAGG ACGAGATGAA GGCGTCTGAG 
GACTTAAAGA AGCATGGTGC CACCGTGCTC ACCGCCCTGG GTGGCATCCT TAAGAAGAAG 
GGGCATCATG AGGCAGAGAT TAAGCCCCTG GCACAGTCGC ATGCCACCAA GCACAAGATC 
CCCGTGAAGT ACCTGGAGTT CATCTCGGAA TGCATCATCC AGGTTCTGCA GAGCAAGCAT 
CCCGGGGACT TTGGTGCTGA TGCCCAGGGG GCCATGAACA AGGCCCTGGA GCTGTTCCGG 
AAGGACATGG CCTCCAACTA CAAGGAGCTG GGCTTCCAGG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005359.1
CDS	73..537
Translation	MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQVLQSKHPGDFGADAQGAMNKALELFRKDMASNYKELGFQG

Target ORF information:

RefSeq Version	NM_005368.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myoglobin (MB), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005368.3

ORF Insert Sequence:

ATGGGGCTCA GCGACGGGGA ATGGCAGTTG GTGCTGAACG TCTGGGGGAA GGTGGAGGCT 
GACATCCCAG GCCATGGGCA GGAAGTCCTC ATCAGGCTCT TTAAGGGTCA CCCAGAGACT 
CTGGAGAAGT TTGACAAGTT CAAGCACCTG AAGTCAGAGG ACGAGATGAA GGCGTCTGAG 
GACTTAAAGA AGCATGGTGC CACCGTGCTC ACCGCCCTGG GTGGCATCCT TAAGAAGAAG 
GGGCATCATG AGGCAGAGAT TAAGCCCCTG GCACAGTCGC ATGCCACCAA GCACAAGATC 
CCCGTGAAGT ACCTGGAGTT CATCTCGGAA TGCATCATCC AGGTTCTGCA GAGCAAGCAT 
CCCGGGGACT TTGGTGCTGA TGCCCAGGGG GCCATGAACA AGGCCCTGGA GCTGTTCCGG 
AAGGACATGG CCTCCAACTA CAAGGAGCTG GGCTTCCAGG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MB ( NM_203377.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_976311.1 Homo sapiens myoglobin (MB), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu20046
Clone ID Related Accession (Same CDS sequence)	NM_203378.1 , NM_005368.3 , NM_203377.1 , NM_005368.2 , NM_001362846.1 , XM_005261605.2 , NM_001362846.2
Accession Version	NM_203377.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Homo sapiens(human)
Product	myoglobin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB140824.1, BQ956082.1 and BC014547.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1-4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279842.10895.1, ERR279869.3509.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2153427 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCTCA GCGACGGGGA ATGGCAGTTG GTGCTGAACG TCTGGGGGAA GGTGGAGGCT 
GACATCCCAG GCCATGGGCA GGAAGTCCTC ATCAGGCTCT TTAAGGGTCA CCCAGAGACT 
CTGGAGAAGT TTGACAAGTT CAAGCACCTG AAGTCAGAGG ACGAGATGAA GGCGTCTGAG 
GACTTAAAGA AGCATGGTGC CACCGTGCTC ACCGCCCTGG GTGGCATCCT TAAGAAGAAG 
GGGCATCATG AGGCAGAGAT TAAGCCCCTG GCACAGTCGC ATGCCACCAA GCACAAGATC 
CCCGTGAAGT ACCTGGAGTT CATCTCGGAA TGCATCATCC AGGTTCTGCA GAGCAAGCAT 
CCCGGGGACT TTGGTGCTGA TGCCCAGGGG GCCATGAACA AGGCCCTGGA GCTGTTCCGG 
AAGGACATGG CCTCCAACTA CAAGGAGCTG GGCTTCCAGG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_976311.1
CDS	173..637
Translation	MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQVLQSKHPGDFGADAQGAMNKALELFRKDMASNYKELGFQG

Target ORF information:

RefSeq Version	NM_203377.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myoglobin (MB), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_203377.1

ORF Insert Sequence:

ATGGGGCTCA GCGACGGGGA ATGGCAGTTG GTGCTGAACG TCTGGGGGAA GGTGGAGGCT 
GACATCCCAG GCCATGGGCA GGAAGTCCTC ATCAGGCTCT TTAAGGGTCA CCCAGAGACT 
CTGGAGAAGT TTGACAAGTT CAAGCACCTG AAGTCAGAGG ACGAGATGAA GGCGTCTGAG 
GACTTAAAGA AGCATGGTGC CACCGTGCTC ACCGCCCTGG GTGGCATCCT TAAGAAGAAG 
GGGCATCATG AGGCAGAGAT TAAGCCCCTG GCACAGTCGC ATGCCACCAA GCACAAGATC 
CCCGTGAAGT ACCTGGAGTT CATCTCGGAA TGCATCATCC AGGTTCTGCA GAGCAAGCAT 
CCCGGGGACT TTGGTGCTGA TGCCCAGGGG GCCATGAACA AGGCCCTGGA GCTGTTCCGG 
AAGGACATGG CCTCCAACTA CAAGGAGCTG GGCTTCCAGG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MB ( NM_005368.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_005359.1 Homo sapiens myoglobin (MB), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20046
Clone ID Related Accession (Same CDS sequence)	NM_203378.1 , NM_005368.3 , NM_203377.1 , NM_005368.2 , NM_001362846.1 , XM_005261605.2 , NM_001362846.2
Accession Version	NM_005368.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506700800000
Organism	Homo sapiens(human)
Product	myoglobin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU585249.1, BQ956082.1 and BC014547.1. On Mar 4, 2004 this sequence version replaced NM_005368.1. Transcript Variant: This variant (1) represents the longest variant. All three variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014547.1, ERR279848.2709.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2153307 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCTCA GCGACGGGGA ATGGCAGTTG GTGCTGAACG TCTGGGGGAA GGTGGAGGCT 
GACATCCCAG GCCATGGGCA GGAAGTCCTC ATCAGGCTCT TTAAGGGTCA CCCAGAGACT 
CTGGAGAAGT TTGACAAGTT CAAGCACCTG AAGTCAGAGG ACGAGATGAA GGCGTCTGAG 
GACTTAAAGA AGCATGGTGC CACCGTGCTC ACCGCCCTGG GTGGCATCCT TAAGAAGAAG 
GGGCATCATG AGGCAGAGAT TAAGCCCCTG GCACAGTCGC ATGCCACCAA GCACAAGATC 
CCCGTGAAGT ACCTGGAGTT CATCTCGGAA TGCATCATCC AGGTTCTGCA GAGCAAGCAT 
CCCGGGGACT TTGGTGCTGA TGCCCAGGGG GCCATGAACA AGGCCCTGGA GCTGTTCCGG 
AAGGACATGG CCTCCAACTA CAAGGAGCTG GGCTTCCAGG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005359.1
CDS	81..545
Translation	MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQVLQSKHPGDFGADAQGAMNKALELFRKDMASNYKELGFQG

Target ORF information:

RefSeq Version	NM_005368.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myoglobin (MB), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005368.2

ORF Insert Sequence:

ATGGGGCTCA GCGACGGGGA ATGGCAGTTG GTGCTGAACG TCTGGGGGAA GGTGGAGGCT 
GACATCCCAG GCCATGGGCA GGAAGTCCTC ATCAGGCTCT TTAAGGGTCA CCCAGAGACT 
CTGGAGAAGT TTGACAAGTT CAAGCACCTG AAGTCAGAGG ACGAGATGAA GGCGTCTGAG 
GACTTAAAGA AGCATGGTGC CACCGTGCTC ACCGCCCTGG GTGGCATCCT TAAGAAGAAG 
GGGCATCATG AGGCAGAGAT TAAGCCCCTG GCACAGTCGC ATGCCACCAA GCACAAGATC 
CCCGTGAAGT ACCTGGAGTT CATCTCGGAA TGCATCATCC AGGTTCTGCA GAGCAAGCAT 
CCCGGGGACT TTGGTGCTGA TGCCCAGGGG GCCATGAACA AGGCCCTGGA GCTGTTCCGG 
AAGGACATGG CCTCCAACTA CAAGGAGCTG GGCTTCCAGG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MB ( NM_001362846.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001349775.1 Homo sapiens myoglobin (MB), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu20046
Clone ID Related Accession (Same CDS sequence)	NM_203378.1 , NM_005368.3 , NM_203377.1 , NM_005368.2 , NM_001362846.1 , XM_005261605.2 , NM_001362846.2
Accession Version	NM_001362846.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1551196800000
Organism	Homo sapiens(human)
Product	myoglobin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL049747.1 and AL022334.1. On May 1, 2018 this sequence version replaced XM_005261605.3. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1-4 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279875.2836.1, SRR5189655.161818.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2153427 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCTCA GCGACGGGGA ATGGCAGTTG GTGCTGAACG TCTGGGGGAA GGTGGAGGCT 
GACATCCCAG GCCATGGGCA GGAAGTCCTC ATCAGGCTCT TTAAGGGTCA CCCAGAGACT 
CTGGAGAAGT TTGACAAGTT CAAGCACCTG AAGTCAGAGG ACGAGATGAA GGCGTCTGAG 
GACTTAAAGA AGCATGGTGC CACCGTGCTC ACCGCCCTGG GTGGCATCCT TAAGAAGAAG 
GGGCATCATG AGGCAGAGAT TAAGCCCCTG GCACAGTCGC ATGCCACCAA GCACAAGATC 
CCCGTGAAGT ACCTGGAGTT CATCTCGGAA TGCATCATCC AGGTTCTGCA GAGCAAGCAT 
CCCGGGGACT TTGGTGCTGA TGCCCAGGGG GCCATGAACA AGGCCCTGGA GCTGTTCCGG 
AAGGACATGG CCTCCAACTA CAAGGAGCTG GGCTTCCAGG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349775.1
CDS	93..557
Translation	MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQVLQSKHPGDFGADAQGAMNKALELFRKDMASNYKELGFQG

Target ORF information:

RefSeq Version	NM_001362846.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myoglobin (MB), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362846.1

ORF Insert Sequence:

ATGGGGCTCA GCGACGGGGA ATGGCAGTTG GTGCTGAACG TCTGGGGGAA GGTGGAGGCT 
GACATCCCAG GCCATGGGCA GGAAGTCCTC ATCAGGCTCT TTAAGGGTCA CCCAGAGACT 
CTGGAGAAGT TTGACAAGTT CAAGCACCTG AAGTCAGAGG ACGAGATGAA GGCGTCTGAG 
GACTTAAAGA AGCATGGTGC CACCGTGCTC ACCGCCCTGG GTGGCATCCT TAAGAAGAAG 
GGGCATCATG AGGCAGAGAT TAAGCCCCTG GCACAGTCGC ATGCCACCAA GCACAAGATC 
CCCGTGAAGT ACCTGGAGTT CATCTCGGAA TGCATCATCC AGGTTCTGCA GAGCAAGCAT 
CCCGGGGACT TTGGTGCTGA TGCCCAGGGG GCCATGAACA AGGCCCTGGA GCTGTTCCGG 
AAGGACATGG CCTCCAACTA CAAGGAGCTG GGCTTCCAGG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MB ( NM_001362846.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001349775.1 Homo sapiens myoglobin (MB), transcript variant 4, mRNA.

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CloneID	OHu20046
Clone ID Related Accession (Same CDS sequence)	NM_203378.1 , NM_005368.3 , NM_203377.1 , NM_005368.2 , NM_001362846.1 , XM_005261605.2 , NM_001362846.2
Accession Version	NM_001362846.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577721600000
Organism	Homo sapiens(human)
Product	myoglobin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL049747.1 and AL022334.1. On May 31, 2019 this sequence version replaced NM_001362846.1. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1-4 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.407576.1, SRR7346977.465445.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2153427 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCTCA GCGACGGGGA ATGGCAGTTG GTGCTGAACG TCTGGGGGAA GGTGGAGGCT 
GACATCCCAG GCCATGGGCA GGAAGTCCTC ATCAGGCTCT TTAAGGGTCA CCCAGAGACT 
CTGGAGAAGT TTGACAAGTT CAAGCACCTG AAGTCAGAGG ACGAGATGAA GGCGTCTGAG 
GACTTAAAGA AGCATGGTGC CACCGTGCTC ACCGCCCTGG GTGGCATCCT TAAGAAGAAG 
GGGCATCATG AGGCAGAGAT TAAGCCCCTG GCACAGTCGC ATGCCACCAA GCACAAGATC 
CCCGTGAAGT ACCTGGAGTT CATCTCGGAA TGCATCATCC AGGTTCTGCA GAGCAAGCAT 
CCCGGGGACT TTGGTGCTGA TGCCCAGGGG GCCATGAACA AGGCCCTGGA GCTGTTCCGG 
AAGGACATGG CCTCCAACTA CAAGGAGCTG GGCTTCCAGG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349775.1
CDS	61..525
Translation	MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQVLQSKHPGDFGADAQGAMNKALELFRKDMASNYKELGFQG

Target ORF information:

RefSeq Version	NM_001362846.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myoglobin (MB), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362846.2

ORF Insert Sequence:

ATGGGGCTCA GCGACGGGGA ATGGCAGTTG GTGCTGAACG TCTGGGGGAA GGTGGAGGCT 
GACATCCCAG GCCATGGGCA GGAAGTCCTC ATCAGGCTCT TTAAGGGTCA CCCAGAGACT 
CTGGAGAAGT TTGACAAGTT CAAGCACCTG AAGTCAGAGG ACGAGATGAA GGCGTCTGAG 
GACTTAAAGA AGCATGGTGC CACCGTGCTC ACCGCCCTGG GTGGCATCCT TAAGAAGAAG 
GGGCATCATG AGGCAGAGAT TAAGCCCCTG GCACAGTCGC ATGCCACCAA GCACAAGATC 
CCCGTGAAGT ACCTGGAGTT CATCTCGGAA TGCATCATCC AGGTTCTGCA GAGCAAGCAT 
CCCGGGGACT TTGGTGCTGA TGCCCAGGGG GCCATGAACA AGGCCCTGGA GCTGTTCCGG 
AAGGACATGG CCTCCAACTA CAAGGAGCTG GGCTTCCAGG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MB ( XM_005261605.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005261662.1 Homo sapiens myoglobin (MB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu20046
Clone ID Related Accession (Same CDS sequence)	NM_203378.1 , NM_005368.3 , NM_203377.1 , NM_005368.2 , NM_001362846.1 , XM_005261605.2 , NM_001362846.2
Accession Version	XM_005261605.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	myoglobin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 3, 2014 this sequence version replaced XM_005261605.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTCA GCGACGGGGA ATGGCAGTTG GTGCTGAACG TCTGGGGGAA GGTGGAGGCT 
GACATCCCAG GCCATGGGCA GGAAGTCCTC ATCAGGCTCT TTAAGGGTCA CCCAGAGACT 
CTGGAGAAGT TTGACAAGTT CAAGCACCTG AAGTCAGAGG ACGAGATGAA GGCGTCTGAG 
GACTTAAAGA AGCATGGTGC CACCGTGCTC ACCGCCCTGG GTGGCATCCT TAAGAAGAAG 
GGGCATCATG AGGCAGAGAT TAAGCCCCTG GCACAGTCGC ATGCCACCAA GCACAAGATC 
CCCGTGAAGT ACCTGGAGTT CATCTCGGAA TGCATCATCC AGGTTCTGCA GAGCAAGCAT 
CCCGGGGACT TTGGTGCTGA TGCCCAGGGG GCCATGAACA AGGCCCTGGA GCTGTTCCGG 
AAGGACATGG CCTCCAACTA CAAGGAGCTG GGCTTCCAGG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005261662.1
CDS	117..581
Translation	MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPVKYLEFISECIIQVLQSKHPGDFGADAQGAMNKALELFRKDMASNYKELGFQG

Target ORF information:

RefSeq Version	XM_005261605.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myoglobin (MB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005261605.2

ORF Insert Sequence:

ATGGGGCTCA GCGACGGGGA ATGGCAGTTG GTGCTGAACG TCTGGGGGAA GGTGGAGGCT 
GACATCCCAG GCCATGGGCA GGAAGTCCTC ATCAGGCTCT TTAAGGGTCA CCCAGAGACT 
CTGGAGAAGT TTGACAAGTT CAAGCACCTG AAGTCAGAGG ACGAGATGAA GGCGTCTGAG 
GACTTAAAGA AGCATGGTGC CACCGTGCTC ACCGCCCTGG GTGGCATCCT TAAGAAGAAG 
GGGCATCATG AGGCAGAGAT TAAGCCCCTG GCACAGTCGC ATGCCACCAA GCACAAGATC 
CCCGTGAAGT ACCTGGAGTT CATCTCGGAA TGCATCATCC AGGTTCTGCA GAGCAAGCAT 
CCCGGGGACT TTGGTGCTGA TGCCCAGGGG GCCATGAACA AGGCCCTGGA GCTGTTCCGG 
AAGGACATGG CCTCCAACTA CAAGGAGCTG GGCTTCCAGG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Myoglobinopathy is an adult-onset autosomal dominant myopathy with characteristic sarcoplasmic inclusions.
Nature communications10(1)1396(2019 03)
Olivé M,Engvall M,Ravenscroft G,Cabrera-Serrano M,Jiao H,Bortolotti CA,Pignataro M,Lambrughi M,Jiang H,Forrest ARR,Benseny-Cases N,Hofbauer S,Obinger C,Battistuzzi G,Bellei M,Borsari M,Di Rocco G,Viola HM,Hool LC,Cladera J,Lagerstedt-Robinson K,Xiang F,Wredenberg A,Miralles F,Baiges JJ,Malfatti E,Romero NB,Streichenberger N,Vial C,Claeys KG,Straathof CSM,Goris A,Freyer C,Lammens M,Bassez G,Kere J,Clemente P,Sejersen T,Udd B,Vidal N,Ferrer I,Edström L,Wedell A,Laing NG

Dynamical comparison between myoglobin and hemoglobin.
Proteins86(11)1176-1183(2018 11)
Aharoni R,Tobi D

A Multi-Region Magnetoimpedance-Based Bio-Analytical System for Ultrasensitive Simultaneous Determination of Cardiac Biomarkers Myoglobin and C-Reactive Protein.
Sensors (Basel, Switzerland)18(6)(2018 Jun)
Yang Z,Wang H,Guo P,Ding Y,Lei C,Luo Y

Electroanalysis of myoglobin based on electropolymerized molecularly imprinted polymer poly-o-phenylenediamine and carbon nanotubes/screen printed electrode.
Doklady. Biochemistry and biophysics468(1)213-6(2016 May)
Shumyantseva VV,Bulko TV,Sigolaeva LV,Kuzikov AV,Archakov AI

Myoglobin A79G polymorphism association with exercise-induced skeletal muscle damage.
Genetics and molecular research : GMR15(2)(2016 May)
Cui T,Jiang MS

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MB cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

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