HCN1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HCN1
Entrez Gene ID	348980
Full Name	hyperpolarization activated cyclic nucleotide gated potassium channel 1
Synonyms	BCNG-1,BCNG1,EIEE24,HAC-2
General protein information	Preferred Names hyperpolarization activated cyclic nucleotide gated potassium channel 1 Names potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 brain cyclic nucleotide-gated channel 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5p12
Summary	The membrane protein encoded by this gene is a hyperpolarization-activated cation channel that contributes to the native pacemaker currents in heart and neurons. The encoded protein can homodimerize or heterodimerize with other pore-forming subunits to form a potassium channel. This channel may act as a receptor for sour tastes. [provided by RefSeq, Oct 2011].
Disorder MIM:	602780
Disorder Html:	Epileptic encephalopathy, early infantile, 24, 615871 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_021072.3	NP_066550.2	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
NM_021072.4	NP_066550.2	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	HCN1	NP_066550.2
Danio rerio (zebrafish)	LOC100334297	XP_005169491.1
Rattus norvegicus (Norway rat)	Hcn1	NP_445827.1
Mus musculus (house mouse)	Hcn1	NP_034538.2
Gallus gallus (chicken)	HCN1	XP_429145.3
Xenopus tropicalis (tropical clawed frog)	hcn1	XP_002933077.2
Canis lupus familiaris (dog)	HCN1	XP_855255.1
Bos taurus (cattle)	HCN1	NP_001193509.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene mutational analysis in a cohort of Chinese children with unexplained epilepsy: Identification of a new KCND3 phenotype and novel genes causing Dravet syndrome.
Wang J, Wen Y, Zhang Q, Yu S, Chen Y, Wu X, Zhang Y, Bao X
Seizure6626-30(2019 Mar)

h-Channels Contribute to Divergent Intrinsic Membrane Properties of Supragranular Pyramidal Neurons in Human versus Mouse Cerebral Cortex.
Kalmbach BE, Buchin A, Long B, Close J, Nandi A, Miller JA, Bakken TE, Hodge RD, Chong P, de Frates R, Dai K, Maltzer Z, Nicovich PR, Keene CD, Silbergeld DL, Gwinn RP, Cobbs C, Ko AL, Ojemann JG, Koch C, Anastassiou CA, Lein ES, Ting JT
Neuron100(5)1194-1208.e5(2018 12)

HCN1 mutation spectrum: from neonatal epileptic encephalopathy to benign generalized epilepsy and beyond.
Marini C, Porro A, Rastetter A, Dalle C, Rivolta I, Bauer D, Oegema R, Nava C, Parrini E, Mei D, Mercer C, Dhamija R, Chambers C, Coubes C, Thévenon J, Kuentz P, Julia S, Pasquier L, Dubourg C, Carré W, Rosati A, Melani F, Pisano T, Giardino M, Innes AM, Alembik Y, Scheidecker S, Santos M, Figueiroa S, Garrido C, Fusco C, Frattini D, Spagnoli C, Binda A, Granata T, Ragona F, Freri E, Franceschetti S, Canafoglia L, Castellotti B, Gellera C, Milanesi R, Mancardi MM, Clark DR, Kok F, Helbig KL, Ichikawa S, Sadler L, Neupauerová J, Laššuthova P, Šterbová K, Laridon A, Brilstra E, Koeleman B, Lemke JR, Zara F, Striano P, Soblet J, Smits G, Deconinck N, Barbuti A, DiFrancesco D, LeGuern E, Guerrini R, Santoro B, Hamacher K, Thiel G, Moroni A, DiFrancesco JC, Depienne C
Brain : a journal of neurology141(11)3160-3178(2018 11)

Mechanical transduction of cytoplasmic-to-transmembrane-domain movements in a hyperpolarization-activated cyclic nucleotide-gated cation channel.
Gross C, Saponaro A, Santoro B, Moroni A, Thiel G, Hamacher K
The Journal of biological chemistry293(33)12908-12918(2018 08)

Case-control pharmacogenetic study of HCN1/HCN2 variants and genetic generalized epilepsies.
Wu SZ, Ye H, Yang XG, Lu ZL, Qu Q, Qu J
Clinical and experimental pharmacology & physiology45(3)226-233(2018 03)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HCN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HCN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21741	NM_021072.4 Latest version!	Homo sapiens hyperpolarization activated cyclic nucleotide gated potassium channel 1 (HCN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$629.30 $899.00
View Old Accession Versions >>
OHu21741	NM_021072.3	Homo sapiens hyperpolarization activated cyclic nucleotide gated potassium channel 1 (HCN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$629.30 $899.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HCN1 ( NM_021072.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_066550.2 Homo sapiens hyperpolarization activated cyclic nucleotide gated potassium channel 1 (HCN1), mRNA.

Price & Availability↑

CloneID	OHu21741
Clone ID Related Accession (Same CDS sequence)	NM_021072.3 , NM_021072.4
Accession Version	NM_021072.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1514304000000
Organism	Homo sapiens(human)
Product	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF488549.1, DR003732.1, AF064876.1, AC099514.2 and CK299850.1. This sequence is a reference standard in the RefSeqGene project. On Oct 4, 2011 this sequence version replaced NM_021072.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF488549.1, SRR1803611.278115.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGGAG GCGGCAAGCC CAACTCTTCG TCTAACAGCC GGGACGATGG CAACAGCGTC 
TTCCCCGCCA AGGCGTCCGC GACGGGCGCG GGGCCGGCCG CGGCCGAGAA GCGCCTGGGC 
ACCCCGCCGG GGGGCGGCGG GGCCGGCGCG AAGGAGCACG GCAACTCCGT GTGCTTCAAG 
GTGGACGGCG GTGGCGGCGG TGGCGGCGGC GGCGGCGGCG GCGAGGAGCC GGCGGGGGGC 
TTCGAAGACG CCGAGGGGCC CCGGCGGCAG TACGGCTTCA TGCAGAGGCA GTTCACCTCC 
ATGCTGCAGC CCGGGGTCAA CAAATTCTCC CTCCGCATGT TTGGGAGCCA GAAGGCGGTG 
GAAAAGGAGC AGGAAAGGGT TAAAACTGCA GGCTTCTGGA TTATCCACCC TTACAGTGAT 
TTCAGGTTTT ACTGGGATTT AATAATGCTT ATAATGATGG TTGGAAATCT AGTCATCATA 
CCAGTTGGAA TCACATTCTT TACAGAGCAA ACAACAACAC CATGGATTAT TTTCAATGTG 
GCATCAGATA CAGTTTTCCT ATTGGACCTG ATCATGAATT TTAGGACTGG GACTGTCAAT 
GAAGACAGTT CTGAAATCAT CCTGGACCCC AAAGTGATCA AGATGAATTA TTTAAAAAGC 
TGGTTTGTGG TTGACTTCAT CTCATCCATC CCAGTGGATT ATATCTTTCT TATTGTAGAA 
AAAGGAATGG ATTCTGAAGT TTACAAGACA GCCAGGGCAC TTCGCATTGT GAGGTTTACA 
AAAATTCTCA GTCTCTTGCG TTTATTACGA CTTTCAAGGT TAATTAGATA CATACATCAA 
TGGGAAGAGA TATTCCACAT GACATATGAT CTCGCCAGTG CAGTGGTGAG AATTTTTAAT 
CTCATCGGCA TGATGCTGCT CCTGTGCCAC TGGGATGGTT GTCTTCAGTT CTTAGTACCA 
CTACTGCAGG ACTTCCCACC AGATTGCTGG GTGTCTTTAA ATGAAATGGT TAATGATTCT 
TGGGGAAAGC AGTATTCATA CGCACTCTTC AAAGCTATGA GTCACATGCT GTGCATTGGG 
TATGGAGCCC AAGCCCCAGT CAGCATGTCT GACCTCTGGA TTACCATGCT GAGCATGATC 
GTCGGGGCCA CCTGCTATGC CATGTTTGTC GGCCATGCCA CCGCTTTAAT CCAGTCTCTG 
GATTCTTCGA GGCGGCAGTA TCAAGAGAAG TATAAGCAAG TGGAACAATA CATGTCATTC 
CATAAGTTAC CAGCTGATAT GCGTCAGAAG ATACATGATT ACTATGAACA CAGATACCAA 
GGCAAAATCT TTGATGAGGA AAATATTCTC AATGAACTCA ATGATCCTCT GAGAGAGGAG 
ATAGTCAACT TCAACTGTCG GAAACTGGTG GCTACAATGC CTTTATTTGC TAATGCGGAT 
CCTAATTTTG TGACTGCCAT GCTGAGCAAG TTGAGATTTG AGGTGTTTCA ACCTGGAGAT 
TATATCATAC GAGAAGGAGC CGTGGGTAAA AAAATGTATT TCATTCAACA CGGTGTTGCT 
GGTGTCATTA CAAAATCCAG TAAAGAAATG AAGCTGACAG ATGGCTCTTA CTTTGGAGAG 
ATTTGCCTGC TGACCAAAGG ACGTCGTACT GCCAGTGTTC GAGCTGATAC ATATTGTCGT 
CTTTACTCAC TTTCCGTGGA CAATTTCAAC GAGGTCCTGG AGGAATATCC AATGATGAGG 
AGAGCCTTTG AGACAGTTGC CATTGACCGA CTAGATCGAA TAGGAAAGAA AAATTCAATT 
CTTCTGCAAA AGTTCCAGAA GGATCTGAAC ACTGGTGTTT TCAACAATCA GGAGAACGAA 
ATCCTCAAGC AGATTGTGAA ACATGACAGG GAGATGGTGC AGGCAATCGC TCCCATCAAT 
TATCCTCAAA TGACAACCCT GAATTCCACA TCGTCTACTA CGACCCCGAC CTCCCGCATG 
AGGACACAAT CTCCACCGGT GTACACAGCG ACCAGCCTGT CTCACAGCAA CCTGCACTCC 
CCCAGTCCCA GCACACAGAC CCCCCAGCCA TCAGCCATCC TGTCACCCTG CTCCTACACC 
ACCGCGGTCT GCAGCCCTCC TGTACAGAGC CCTCTGGCCG CTCGAACTTT CCACTATGCC 
TCCCCCACCG CCTCCCAGCT GTCACTCATG CAACAGCAGC CGCAGCAGCA GGTACAGCAG 
TCCCAGCCGC CGCAGACTCA GCCACAGCAG CCGTCCCCGC AGCCACAGAC ACCTGGCAGC 
TCCACGCCGA AAAATGAAGT GCACAAGAGC ACGCAGGCGC TTCACAACAC CAACCTGACC 
CGGGAAGTCA GGCCACTCTC CGCCTCGCAG CCCTCGCTGC CCCATGAGGT GTCCACTCTG 
ATTTCCAGAC CTCATCCCAC TGTGGGCGAG TCCCTGGCCT CCATCCCTCA ACCCGTGACG 
GCGGTCCCCG GAACGGGCCT TCAGGCAGGG GGCAGGAGCA CTGTCCCGCA GCGCGTCACC 
CTCTTCCGAC AGATGTCGTC GGGAGCCATC CCCCCGAACC GAGGAGTCCC TCCAGCACCC 
CCTCCACCAG CAGCTGCTCT TCCAAGAGAA TCTTCCTCAG TCTTAAACAC AGACCCAGAC 
GCAGAAAAGC CACGATTTGC TTCAAATTTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066550.2
CDS	26..2698
Translation	MEGGGKPNSSSNSRDDGNSVFPAKASATGAGPAAAEKRLGTPPGGGGAGAKEHGNSVCFKVDGGGGGGGGGGGGEEPAGGFEDAEGPRRQYGFMQRQFTSMLQPGVNKFSLRMFGSQKAVEKEQERVKTAGFWIIHPYSDFRFYWDLIMLIMMVGNLVIIPVGITFFTEQTTTPWIIFNVASDTVFLLDLIMNFRTGTVNEDSSEIILDPKVIKMNYLKSWFVVDFISSIPVDYIFLIVEKGMDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIFNLIGMMLLLCHWDGCLQFLVPLLQDFPPDCWVSLNEMVNDSWGKQYSYALFKAMSHMLCIGYGAQAPVSMSDLWITMLSMIVGATCYAMFVGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADMRQKIHDYYEHRYQGKIFDEENILNELNDPLREEIVNFNCRKLVATMPLFANADPNFVTAMLSKLRFEVFQPGDYIIREGAVGKKMYFIQHGVAGVITKSSKEMKLTDGSYFGEICLLTKGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILLQKFQKDLNTGVFNNQENEILKQIVKHDREMVQAIAPINYPQMTTLNSTSSTTTPTSRMRTQSPPVYTATSLSHSNLHSPSPSTQTPQPSAILSPCSYTTAVCSPPVQSPLAARTFHYASPTASQLSLMQQQPQQQVQQSQPPQTQPQQPSPQPQTPGSSTPKNEVHKSTQALHNTNLTREVRPLSASQPSLPHEVSTLISRPHPTVGESLASIPQPVTAVPGTGLQAGGRSTVPQRVTLFRQMSSGAIPPNRGVPPAPPPPAAALPRESSSVLNTDPDAEKPRFASNL

Target ORF information:

RefSeq Version	NM_021072.3
Organism	Homo sapiens(human)
Definition	Homo sapiens hyperpolarization activated cyclic nucleotide gated potassium channel 1 (HCN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021072.3

ORF Insert Sequence:

ATGGAAGGAG GCGGCAAGCC CAACTCTTCG TCTAACAGCC GGGACGATGG CAACAGCGTC 
TTCCCCGCCA AGGCGTCCGC GACGGGCGCG GGGCCGGCCG CGGCCGAGAA GCGCCTGGGC 
ACCCCGCCGG GGGGCGGCGG GGCCGGCGCG AAGGAGCACG GCAACTCCGT GTGCTTCAAG 
GTGGACGGCG GTGGCGGCGG TGGCGGCGGC GGCGGCGGCG GCGAGGAGCC GGCGGGGGGC 
TTCGAAGACG CCGAGGGGCC CCGGCGGCAG TACGGCTTCA TGCAGAGGCA GTTCACCTCC 
ATGCTGCAGC CCGGGGTCAA CAAATTCTCC CTCCGCATGT TTGGGAGCCA GAAGGCGGTG 
GAAAAGGAGC AGGAAAGGGT TAAAACTGCA GGCTTCTGGA TTATCCACCC TTACAGTGAT 
TTCAGGTTTT ACTGGGATTT AATAATGCTT ATAATGATGG TTGGAAATCT AGTCATCATA 
CCAGTTGGAA TCACATTCTT TACAGAGCAA ACAACAACAC CATGGATTAT TTTCAATGTG 
GCATCAGATA CAGTTTTCCT ATTGGACCTG ATCATGAATT TTAGGACTGG GACTGTCAAT 
GAAGACAGTT CTGAAATCAT CCTGGACCCC AAAGTGATCA AGATGAATTA TTTAAAAAGC 
TGGTTTGTGG TTGACTTCAT CTCATCCATC CCAGTGGATT ATATCTTTCT TATTGTAGAA 
AAAGGAATGG ATTCTGAAGT TTACAAGACA GCCAGGGCAC TTCGCATTGT GAGGTTTACA 
AAAATTCTCA GTCTCTTGCG TTTATTACGA CTTTCAAGGT TAATTAGATA CATACATCAA 
TGGGAAGAGA TATTCCACAT GACATATGAT CTCGCCAGTG CAGTGGTGAG AATTTTTAAT 
CTCATCGGCA TGATGCTGCT CCTGTGCCAC TGGGATGGTT GTCTTCAGTT CTTAGTACCA 
CTACTGCAGG ACTTCCCACC AGATTGCTGG GTGTCTTTAA ATGAAATGGT TAATGATTCT 
TGGGGAAAGC AGTATTCATA CGCACTCTTC AAAGCTATGA GTCACATGCT GTGCATTGGG 
TATGGAGCCC AAGCCCCAGT CAGCATGTCT GACCTCTGGA TTACCATGCT GAGCATGATC 
GTCGGGGCCA CCTGCTATGC CATGTTTGTC GGCCATGCCA CCGCTTTAAT CCAGTCTCTG 
GATTCTTCGA GGCGGCAGTA TCAAGAGAAG TATAAGCAAG TGGAACAATA CATGTCATTC 
CATAAGTTAC CAGCTGATAT GCGTCAGAAG ATACATGATT ACTATGAACA CAGATACCAA 
GGCAAAATCT TTGATGAGGA AAATATTCTC AATGAACTCA ATGATCCTCT GAGAGAGGAG 
ATAGTCAACT TCAACTGTCG GAAACTGGTG GCTACAATGC CTTTATTTGC TAATGCGGAT 
CCTAATTTTG TGACTGCCAT GCTGAGCAAG TTGAGATTTG AGGTGTTTCA ACCTGGAGAT 
TATATCATAC GAGAAGGAGC CGTGGGTAAA AAAATGTATT TCATTCAACA CGGTGTTGCT 
GGTGTCATTA CAAAATCCAG TAAAGAAATG AAGCTGACAG ATGGCTCTTA CTTTGGAGAG 
ATTTGCCTGC TGACCAAAGG ACGTCGTACT GCCAGTGTTC GAGCTGATAC ATATTGTCGT 
CTTTACTCAC TTTCCGTGGA CAATTTCAAC GAGGTCCTGG AGGAATATCC AATGATGAGG 
AGAGCCTTTG AGACAGTTGC CATTGACCGA CTAGATCGAA TAGGAAAGAA AAATTCAATT 
CTTCTGCAAA AGTTCCAGAA GGATCTGAAC ACTGGTGTTT TCAACAATCA GGAGAACGAA 
ATCCTCAAGC AGATTGTGAA ACATGACAGG GAGATGGTGC AGGCAATCGC TCCCATCAAT 
TATCCTCAAA TGACAACCCT GAATTCCACA TCGTCTACTA CGACCCCGAC CTCCCGCATG 
AGGACACAAT CTCCACCGGT GTACACAGCG ACCAGCCTGT CTCACAGCAA CCTGCACTCC 
CCCAGTCCCA GCACACAGAC CCCCCAGCCA TCAGCCATCC TGTCACCCTG CTCCTACACC 
ACCGCGGTCT GCAGCCCTCC TGTACAGAGC CCTCTGGCCG CTCGAACTTT CCACTATGCC 
TCCCCCACCG CCTCCCAGCT GTCACTCATG CAACAGCAGC CGCAGCAGCA GGTACAGCAG 
TCCCAGCCGC CGCAGACTCA GCCACAGCAG CCGTCCCCGC AGCCACAGAC ACCTGGCAGC 
TCCACGCCGA AAAATGAAGT GCACAAGAGC ACGCAGGCGC TTCACAACAC CAACCTGACC 
CGGGAAGTCA GGCCACTCTC CGCCTCGCAG CCCTCGCTGC CCCATGAGGT GTCCACTCTG 
ATTTCCAGAC CTCATCCCAC TGTGGGCGAG TCCCTGGCCT CCATCCCTCA ACCCGTGACG 
GCGGTCCCCG GAACGGGCCT TCAGGCAGGG GGCAGGAGCA CTGTCCCGCA GCGCGTCACC 
CTCTTCCGAC AGATGTCGTC GGGAGCCATC CCCCCGAACC GAGGAGTCCC TCCAGCACCC 
CCTCCACCAG CAGCTGCTCT TCCAAGAGAA TCTTCCTCAG TCTTAAACAC AGACCCAGAC 
GCAGAAAAGC CACGATTTGC TTCAAATTTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCN1 ( NM_021072.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_066550.2 Homo sapiens hyperpolarization activated cyclic nucleotide gated potassium channel 1 (HCN1), mRNA.

Price & Availability↑

CloneID	OHu21741
Clone ID Related Accession (Same CDS sequence)	NM_021072.3 , NM_021072.4
Accession Version	NM_021072.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577548800000
Organism	Homo sapiens(human)
Product	potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC114975.2, AF488549.1, DR003732.1, AF064876.1, AC099514.2 and CK299850.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_021072.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.278115.1, SRR1803611.207132.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000303230.6/ ENSP00000307342.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAAGGAG GCGGCAAGCC CAACTCTTCG TCTAACAGCC GGGACGATGG CAACAGCGTC 
TTCCCCGCCA AGGCGTCCGC GACGGGCGCG GGGCCGGCCG CGGCCGAGAA GCGCCTGGGC 
ACCCCGCCGG GGGGCGGCGG GGCCGGCGCG AAGGAGCACG GCAACTCCGT GTGCTTCAAG 
GTGGACGGCG GTGGCGGCGG TGGCGGCGGC GGCGGCGGCG GCGAGGAGCC GGCGGGGGGC 
TTCGAAGACG CCGAGGGGCC CCGGCGGCAG TACGGCTTCA TGCAGAGGCA GTTCACCTCC 
ATGCTGCAGC CCGGGGTCAA CAAATTCTCC CTCCGCATGT TTGGGAGCCA GAAGGCGGTG 
GAAAAGGAGC AGGAAAGGGT TAAAACTGCA GGCTTCTGGA TTATCCACCC TTACAGTGAT 
TTCAGGTTTT ACTGGGATTT AATAATGCTT ATAATGATGG TTGGAAATCT AGTCATCATA 
CCAGTTGGAA TCACATTCTT TACAGAGCAA ACAACAACAC CATGGATTAT TTTCAATGTG 
GCATCAGATA CAGTTTTCCT ATTGGACCTG ATCATGAATT TTAGGACTGG GACTGTCAAT 
GAAGACAGTT CTGAAATCAT CCTGGACCCC AAAGTGATCA AGATGAATTA TTTAAAAAGC 
TGGTTTGTGG TTGACTTCAT CTCATCCATC CCAGTGGATT ATATCTTTCT TATTGTAGAA 
AAAGGAATGG ATTCTGAAGT TTACAAGACA GCCAGGGCAC TTCGCATTGT GAGGTTTACA 
AAAATTCTCA GTCTCTTGCG TTTATTACGA CTTTCAAGGT TAATTAGATA CATACATCAA 
TGGGAAGAGA TATTCCACAT GACATATGAT CTCGCCAGTG CAGTGGTGAG AATTTTTAAT 
CTCATCGGCA TGATGCTGCT CCTGTGCCAC TGGGATGGTT GTCTTCAGTT CTTAGTACCA 
CTACTGCAGG ACTTCCCACC AGATTGCTGG GTGTCTTTAA ATGAAATGGT TAATGATTCT 
TGGGGAAAGC AGTATTCATA CGCACTCTTC AAAGCTATGA GTCACATGCT GTGCATTGGG 
TATGGAGCCC AAGCCCCAGT CAGCATGTCT GACCTCTGGA TTACCATGCT GAGCATGATC 
GTCGGGGCCA CCTGCTATGC CATGTTTGTC GGCCATGCCA CCGCTTTAAT CCAGTCTCTG 
GATTCTTCGA GGCGGCAGTA TCAAGAGAAG TATAAGCAAG TGGAACAATA CATGTCATTC 
CATAAGTTAC CAGCTGATAT GCGTCAGAAG ATACATGATT ACTATGAACA CAGATACCAA 
GGCAAAATCT TTGATGAGGA AAATATTCTC AATGAACTCA ATGATCCTCT GAGAGAGGAG 
ATAGTCAACT TCAACTGTCG GAAACTGGTG GCTACAATGC CTTTATTTGC TAATGCGGAT 
CCTAATTTTG TGACTGCCAT GCTGAGCAAG TTGAGATTTG AGGTGTTTCA ACCTGGAGAT 
TATATCATAC GAGAAGGAGC CGTGGGTAAA AAAATGTATT TCATTCAACA CGGTGTTGCT 
GGTGTCATTA CAAAATCCAG TAAAGAAATG AAGCTGACAG ATGGCTCTTA CTTTGGAGAG 
ATTTGCCTGC TGACCAAAGG ACGTCGTACT GCCAGTGTTC GAGCTGATAC ATATTGTCGT 
CTTTACTCAC TTTCCGTGGA CAATTTCAAC GAGGTCCTGG AGGAATATCC AATGATGAGG 
AGAGCCTTTG AGACAGTTGC CATTGACCGA CTAGATCGAA TAGGAAAGAA AAATTCAATT 
CTTCTGCAAA AGTTCCAGAA GGATCTGAAC ACTGGTGTTT TCAACAATCA GGAGAACGAA 
ATCCTCAAGC AGATTGTGAA ACATGACAGG GAGATGGTGC AGGCAATCGC TCCCATCAAT 
TATCCTCAAA TGACAACCCT GAATTCCACA TCGTCTACTA CGACCCCGAC CTCCCGCATG 
AGGACACAAT CTCCACCGGT GTACACAGCG ACCAGCCTGT CTCACAGCAA CCTGCACTCC 
CCCAGTCCCA GCACACAGAC CCCCCAGCCA TCAGCCATCC TGTCACCCTG CTCCTACACC 
ACCGCGGTCT GCAGCCCTCC TGTACAGAGC CCTCTGGCCG CTCGAACTTT CCACTATGCC 
TCCCCCACCG CCTCCCAGCT GTCACTCATG CAACAGCAGC CGCAGCAGCA GGTACAGCAG 
TCCCAGCCGC CGCAGACTCA GCCACAGCAG CCGTCCCCGC AGCCACAGAC ACCTGGCAGC 
TCCACGCCGA AAAATGAAGT GCACAAGAGC ACGCAGGCGC TTCACAACAC CAACCTGACC 
CGGGAAGTCA GGCCACTCTC CGCCTCGCAG CCCTCGCTGC CCCATGAGGT GTCCACTCTG 
ATTTCCAGAC CTCATCCCAC TGTGGGCGAG TCCCTGGCCT CCATCCCTCA ACCCGTGACG 
GCGGTCCCCG GAACGGGCCT TCAGGCAGGG GGCAGGAGCA CTGTCCCGCA GCGCGTCACC 
CTCTTCCGAC AGATGTCGTC GGGAGCCATC CCCCCGAACC GAGGAGTCCC TCCAGCACCC 
CCTCCACCAG CAGCTGCTCT TCCAAGAGAA TCTTCCTCAG TCTTAAACAC AGACCCAGAC 
GCAGAAAAGC CACGATTTGC TTCAAATTTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066550.2
CDS	288..2960
Translation	MEGGGKPNSSSNSRDDGNSVFPAKASATGAGPAAAEKRLGTPPGGGGAGAKEHGNSVCFKVDGGGGGGGGGGGGEEPAGGFEDAEGPRRQYGFMQRQFTSMLQPGVNKFSLRMFGSQKAVEKEQERVKTAGFWIIHPYSDFRFYWDLIMLIMMVGNLVIIPVGITFFTEQTTTPWIIFNVASDTVFLLDLIMNFRTGTVNEDSSEIILDPKVIKMNYLKSWFVVDFISSIPVDYIFLIVEKGMDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIFNLIGMMLLLCHWDGCLQFLVPLLQDFPPDCWVSLNEMVNDSWGKQYSYALFKAMSHMLCIGYGAQAPVSMSDLWITMLSMIVGATCYAMFVGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADMRQKIHDYYEHRYQGKIFDEENILNELNDPLREEIVNFNCRKLVATMPLFANADPNFVTAMLSKLRFEVFQPGDYIIREGAVGKKMYFIQHGVAGVITKSSKEMKLTDGSYFGEICLLTKGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILLQKFQKDLNTGVFNNQENEILKQIVKHDREMVQAIAPINYPQMTTLNSTSSTTTPTSRMRTQSPPVYTATSLSHSNLHSPSPSTQTPQPSAILSPCSYTTAVCSPPVQSPLAARTFHYASPTASQLSLMQQQPQQQVQQSQPPQTQPQQPSPQPQTPGSSTPKNEVHKSTQALHNTNLTREVRPLSASQPSLPHEVSTLISRPHPTVGESLASIPQPVTAVPGTGLQAGGRSTVPQRVTLFRQMSSGAIPPNRGVPPAPPPPAAALPRESSSVLNTDPDAEKPRFASNL

Target ORF information:

RefSeq Version	NM_021072.4
Organism	Homo sapiens(human)
Definition	Homo sapiens hyperpolarization activated cyclic nucleotide gated potassium channel 1 (HCN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021072.4

ORF Insert Sequence:

ATGGAAGGAG GCGGCAAGCC CAACTCTTCG TCTAACAGCC GGGACGATGG CAACAGCGTC 
TTCCCCGCCA AGGCGTCCGC GACGGGCGCG GGGCCGGCCG CGGCCGAGAA GCGCCTGGGC 
ACCCCGCCGG GGGGCGGCGG GGCCGGCGCG AAGGAGCACG GCAACTCCGT GTGCTTCAAG 
GTGGACGGCG GTGGCGGCGG TGGCGGCGGC GGCGGCGGCG GCGAGGAGCC GGCGGGGGGC 
TTCGAAGACG CCGAGGGGCC CCGGCGGCAG TACGGCTTCA TGCAGAGGCA GTTCACCTCC 
ATGCTGCAGC CCGGGGTCAA CAAATTCTCC CTCCGCATGT TTGGGAGCCA GAAGGCGGTG 
GAAAAGGAGC AGGAAAGGGT TAAAACTGCA GGCTTCTGGA TTATCCACCC TTACAGTGAT 
TTCAGGTTTT ACTGGGATTT AATAATGCTT ATAATGATGG TTGGAAATCT AGTCATCATA 
CCAGTTGGAA TCACATTCTT TACAGAGCAA ACAACAACAC CATGGATTAT TTTCAATGTG 
GCATCAGATA CAGTTTTCCT ATTGGACCTG ATCATGAATT TTAGGACTGG GACTGTCAAT 
GAAGACAGTT CTGAAATCAT CCTGGACCCC AAAGTGATCA AGATGAATTA TTTAAAAAGC 
TGGTTTGTGG TTGACTTCAT CTCATCCATC CCAGTGGATT ATATCTTTCT TATTGTAGAA 
AAAGGAATGG ATTCTGAAGT TTACAAGACA GCCAGGGCAC TTCGCATTGT GAGGTTTACA 
AAAATTCTCA GTCTCTTGCG TTTATTACGA CTTTCAAGGT TAATTAGATA CATACATCAA 
TGGGAAGAGA TATTCCACAT GACATATGAT CTCGCCAGTG CAGTGGTGAG AATTTTTAAT 
CTCATCGGCA TGATGCTGCT CCTGTGCCAC TGGGATGGTT GTCTTCAGTT CTTAGTACCA 
CTACTGCAGG ACTTCCCACC AGATTGCTGG GTGTCTTTAA ATGAAATGGT TAATGATTCT 
TGGGGAAAGC AGTATTCATA CGCACTCTTC AAAGCTATGA GTCACATGCT GTGCATTGGG 
TATGGAGCCC AAGCCCCAGT CAGCATGTCT GACCTCTGGA TTACCATGCT GAGCATGATC 
GTCGGGGCCA CCTGCTATGC CATGTTTGTC GGCCATGCCA CCGCTTTAAT CCAGTCTCTG 
GATTCTTCGA GGCGGCAGTA TCAAGAGAAG TATAAGCAAG TGGAACAATA CATGTCATTC 
CATAAGTTAC CAGCTGATAT GCGTCAGAAG ATACATGATT ACTATGAACA CAGATACCAA 
GGCAAAATCT TTGATGAGGA AAATATTCTC AATGAACTCA ATGATCCTCT GAGAGAGGAG 
ATAGTCAACT TCAACTGTCG GAAACTGGTG GCTACAATGC CTTTATTTGC TAATGCGGAT 
CCTAATTTTG TGACTGCCAT GCTGAGCAAG TTGAGATTTG AGGTGTTTCA ACCTGGAGAT 
TATATCATAC GAGAAGGAGC CGTGGGTAAA AAAATGTATT TCATTCAACA CGGTGTTGCT 
GGTGTCATTA CAAAATCCAG TAAAGAAATG AAGCTGACAG ATGGCTCTTA CTTTGGAGAG 
ATTTGCCTGC TGACCAAAGG ACGTCGTACT GCCAGTGTTC GAGCTGATAC ATATTGTCGT 
CTTTACTCAC TTTCCGTGGA CAATTTCAAC GAGGTCCTGG AGGAATATCC AATGATGAGG 
AGAGCCTTTG AGACAGTTGC CATTGACCGA CTAGATCGAA TAGGAAAGAA AAATTCAATT 
CTTCTGCAAA AGTTCCAGAA GGATCTGAAC ACTGGTGTTT TCAACAATCA GGAGAACGAA 
ATCCTCAAGC AGATTGTGAA ACATGACAGG GAGATGGTGC AGGCAATCGC TCCCATCAAT 
TATCCTCAAA TGACAACCCT GAATTCCACA TCGTCTACTA CGACCCCGAC CTCCCGCATG 
AGGACACAAT CTCCACCGGT GTACACAGCG ACCAGCCTGT CTCACAGCAA CCTGCACTCC 
CCCAGTCCCA GCACACAGAC CCCCCAGCCA TCAGCCATCC TGTCACCCTG CTCCTACACC 
ACCGCGGTCT GCAGCCCTCC TGTACAGAGC CCTCTGGCCG CTCGAACTTT CCACTATGCC 
TCCCCCACCG CCTCCCAGCT GTCACTCATG CAACAGCAGC CGCAGCAGCA GGTACAGCAG 
TCCCAGCCGC CGCAGACTCA GCCACAGCAG CCGTCCCCGC AGCCACAGAC ACCTGGCAGC 
TCCACGCCGA AAAATGAAGT GCACAAGAGC ACGCAGGCGC TTCACAACAC CAACCTGACC 
CGGGAAGTCA GGCCACTCTC CGCCTCGCAG CCCTCGCTGC CCCATGAGGT GTCCACTCTG 
ATTTCCAGAC CTCATCCCAC TGTGGGCGAG TCCCTGGCCT CCATCCCTCA ACCCGTGACG 
GCGGTCCCCG GAACGGGCCT TCAGGCAGGG GGCAGGAGCA CTGTCCCGCA GCGCGTCACC 
CTCTTCCGAC AGATGTCGTC GGGAGCCATC CCCCCGAACC GAGGAGTCCC TCCAGCACCC 
CCTCCACCAG CAGCTGCTCT TCCAAGAGAA TCTTCCTCAG TCTTAAACAC AGACCCAGAC 
GCAGAAAAGC CACGATTTGC TTCAAATTTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene mutational analysis in a cohort of Chinese children with unexplained epilepsy: Identification of a new KCND3 phenotype and novel genes causing Dravet syndrome.
Seizure6626-30(2019 Mar)
Wang J,Wen Y,Zhang Q,Yu S,Chen Y,Wu X,Zhang Y,Bao X

h-Channels Contribute to Divergent Intrinsic Membrane Properties of Supragranular Pyramidal Neurons in Human versus Mouse Cerebral Cortex.
Neuron100(5)1194-1208.e5(2018 12)
Kalmbach BE,Buchin A,Long B,Close J,Nandi A,Miller JA,Bakken TE,Hodge RD,Chong P,de Frates R,Dai K,Maltzer Z,Nicovich PR,Keene CD,Silbergeld DL,Gwinn RP,Cobbs C,Ko AL,Ojemann JG,Koch C,Anastassiou CA,Lein ES,Ting JT

HCN1 mutation spectrum: from neonatal epileptic encephalopathy to benign generalized epilepsy and beyond.
Brain : a journal of neurology141(11)3160-3178(2018 11)
Marini C,Porro A,Rastetter A,Dalle C,Rivolta I,Bauer D,Oegema R,Nava C,Parrini E,Mei D,Mercer C,Dhamija R,Chambers C,Coubes C,Thévenon J,Kuentz P,Julia S,Pasquier L,Dubourg C,Carré W,Rosati A,Melani F,Pisano T,Giardino M,Innes AM,Alembik Y,Scheidecker S,Santos M,Figueiroa S,Garrido C,Fusco C,Frattini D,Spagnoli C,Binda A,Granata T,Ragona F,Freri E,Franceschetti S,Canafoglia L,Castellotti B,Gellera C,Milanesi R,Mancardi MM,Clark DR,Kok F,Helbig KL,Ichikawa S,Sadler L,Neupauerová J,Laššuthova P,Šterbová K,Laridon A,Brilstra E,Koeleman B,Lemke JR,Zara F,Striano P,Soblet J,Smits G,Deconinck N,Barbuti A,DiFrancesco D,LeGuern E,Guerrini R,Santoro B,Hamacher K,Thiel G,Moroni A,DiFrancesco JC,Depienne C

Mechanical transduction of cytoplasmic-to-transmembrane-domain movements in a hyperpolarization-activated cyclic nucleotide-gated cation channel.
The Journal of biological chemistry293(33)12908-12918(2018 08)
Gross C,Saponaro A,Santoro B,Moroni A,Thiel G,Hamacher K

Case-control pharmacogenetic study of HCN1/HCN2 variants and genetic generalized epilepsies.
Clinical and experimental pharmacology & physiology45(3)226-233(2018 03)
Wu SZ,Ye H,Yang XG,Lu ZL,Qu Q,Qu J

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HCN1 cDNA ORF clone, Homo sapiens(human)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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