MYLPF cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYLPF
Entrez Gene ID	29895
Full Name	myosin light chain, phosphorylatable, fast skeletal muscle
Synonyms	HUMMLC2B,MLC2B,MRLC2,MYL11
General protein information	Preferred Names myosin light chain, phosphorylatable, fast skeletal muscle Names myosin regulatory light chain 2, skeletal muscle isoform fast skeletal myosin light chain 2 myosin light chain 2 myosin, light chain 11, regulatory
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p11.2
Disorder MIM:	617378

mRNA and Protein(s)

mRNA	Protein	Name
NM_001324458.2	NP_001311387.1	myosin regulatory light chain 2, skeletal muscle isoform
NM_001324459.1	NP_001311388.1	myosin regulatory light chain 2, skeletal muscle isoform
NM_013292.4	NP_037424.2	myosin regulatory light chain 2, skeletal muscle isoform
NM_013292.3	NP_037424.2	myosin regulatory light chain 2, skeletal muscle isoform
NM_013292.5	NP_037424.2	myosin regulatory light chain 2, skeletal muscle isoform
NM_001324458.1	NP_001311387.1	myosin regulatory light chain 2, skeletal muscle isoform

Pathways from BioSystems

Homologies

Homo sapiens (human)	MYLPF	NP_037424.2
Xenopus tropicalis (tropical clawed frog)	mylpf	NP_989158.1
Danio rerio (zebrafish)	mylpfa	NP_571263.1
Canis lupus familiaris (dog)	MYLPF	XP_536899.2
Bos taurus (cattle)	MYLPF	NP_001069115.1
Mus musculus (house mouse)	Mylpf	NP_058034.1
Rattus norvegicus (Norway rat)	Mylpf	NP_036737.1
Danio rerio (zebrafish)	mylpfb	NP_001004668.1
Caenorhabditis elegans (roundworm)	mlc-2	NP_510828.1
Caenorhabditis elegans (roundworm)	mlc-1	NP_510829.2

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of myosin II regulatory light chain isoforms in HeLa cells.
Kondo T, Okada M, Kunihiro K, Takahashi M, Yaoita Y, Hosoya H, Hamao K
Cytoskeleton (Hoboken, N.J.)72(12)609-20(2015 Dec)

Human fast skeletal myosin light chain 2 cDNA: isolation, tissue specific expression of the single copy gene, comparative sequence analysis of isoforms and evolutionary relationships.
Sachdev S, Raychowdhury MK, Sarkar S
DNA sequence : the journal of DNA sequencing and mapping14(5)339-50(2003 Oct)

The nucleotide sequence of a rat myosin light chain 2 gene.
Nudel U, Calvo JM, Shani M, Levy Z
Nucleic acids research12(18)7175-86(1984 Sep)

Cloning and characterization of cDNA sequences corresponding to myosin light chains 1, 2, and 3, troponin-C, troponin-T, alpha-tropomyosin, and alpha-actin.
Garfinkel LI, Periasamy M, Nadal-Ginard B
The Journal of biological chemistry257(18)11078-86(1982 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MYLPF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYLPF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31266	NM_001324458.2 Latest version!	Homo sapiens myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu31266	NM_001324459.1 Latest version!	Homo sapiens myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu31266	NM_013292.5 Latest version!	Homo sapiens myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
View Old Accession Versions >>
OHu31266	NM_013292.3	Homo sapiens myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu31266	NM_013292.4	Homo sapiens myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu31266	NM_001324458.1	Homo sapiens myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYLPF ( NM_001324458.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001311387.1 Homo sapiens myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu31266
Clone ID Related Accession (Same CDS sequence)	NM_001324458.2 , NM_001324459.1 , NM_013292.4 , NM_013292.3 , NM_013292.5 , NM_001324458.1
Accession Version	NM_001324458.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577808000000
Organism	Homo sapiens(human)
Product	myosin regulatory light chain 2, skeletal muscle isoform
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC127457.3, BI837593.1, BC012571.1 and AA662706.1. On May 31, 2019 this sequence version replaced NM_001324458.1. Transcript Variant: This variant (2) and variants 1 and 3 all encode the same protein. ##Evidence-Data-START## Transcript exon combination :: BI837593.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCACCCA AGAGGGCCAA GAGAAGGACA GTAGAGGGCG GAAGCTCCAG CGTCTTCTCC 
ATGTTCGACC AGACTCAGAT CCAGGAGTTC AAAGAGGCCT TCACTGTGAT CGACCAGAAC 
CGTGATGGTA TTATAGACAA GGAGGACCTT CGGGACACCT TCGCAGCCAT GGGCCGCCTC 
AATGTGAAGA ATGAGGAGTT GGATGCCATG ATGAAGGAAG CCAGCGGTCC CATCAACTTC 
ACCGTCTTCC TGACCATGTT CGGGGAGAAG CTCAAGGGTG CCGACCCTGA GGATGTGATC 
ACCGGAGCCT TCAAGGTCTT GGACCCTGAG GGAAAGGGCA CCATCAAGAA GAAGTTCCTG 
GAGGAGCTGC TGACCACGCA GTGTGACCGC TTCTCCCAGG AGGAGATCAA GAACATGTGG 
GCGGCCTTCC CCCCCGACGT GGGCGGCAAC GTCGACTACA AAAACATCTG CTACGTCATC 
ACGCACGGCG ACGCCAAGGA CCAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001311387.1
CDS	243..752
Translation	MAPKRAKRRTVEGGSSSVFSMFDQTQIQEFKEAFTVIDQNRDGIIDKEDLRDTFAAMGRLNVKNEELDAMMKEASGPINFTVFLTMFGEKLKGADPEDVITGAFKVLDPEGKGTIKKKFLEELLTTQCDRFSQEEIKNMWAAFPPDVGGNVDYKNICYVITHGDAKDQE

Target ORF information:

RefSeq Version	NM_001324458.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001324458.2

ORF Insert Sequence:

ATGGCACCCA AGAGGGCCAA GAGAAGGACA GTAGAGGGCG GAAGCTCCAG CGTCTTCTCC 
ATGTTCGACC AGACTCAGAT CCAGGAGTTC AAAGAGGCCT TCACTGTGAT CGACCAGAAC 
CGTGATGGTA TTATAGACAA GGAGGACCTT CGGGACACCT TCGCAGCCAT GGGCCGCCTC 
AATGTGAAGA ATGAGGAGTT GGATGCCATG ATGAAGGAAG CCAGCGGTCC CATCAACTTC 
ACCGTCTTCC TGACCATGTT CGGGGAGAAG CTCAAGGGTG CCGACCCTGA GGATGTGATC 
ACCGGAGCCT TCAAGGTCTT GGACCCTGAG GGAAAGGGCA CCATCAAGAA GAAGTTCCTG 
GAGGAGCTGC TGACCACGCA GTGTGACCGC TTCTCCCAGG AGGAGATCAA GAACATGTGG 
GCGGCCTTCC CCCCCGACGT GGGCGGCAAC GTCGACTACA AAAACATCTG CTACGTCATC 
ACGCACGGCG ACGCCAAGGA CCAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYLPF ( NM_001324459.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001311388.1 Homo sapiens myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu31266
Clone ID Related Accession (Same CDS sequence)	NM_001324458.2 , NM_001324459.1 , NM_013292.4 , NM_013292.3 , NM_013292.5 , NM_001324458.1
Accession Version	NM_001324459.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577462400000
Organism	Homo sapiens(human)
Product	myosin regulatory light chain 2, skeletal muscle isoform
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BI828249.1, BC012571.1 and AA662706.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (3) and variants 1 and 2 all encode the same protein. ##Evidence-Data-START## Transcript exon combination :: BI828249.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151119, SAMEA2158188 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCACCCA AGAGGGCCAA GAGAAGGACA GTAGAGGGCG GAAGCTCCAG CGTCTTCTCC 
ATGTTCGACC AGACTCAGAT CCAGGAGTTC AAAGAGGCCT TCACTGTGAT CGACCAGAAC 
CGTGATGGTA TTATAGACAA GGAGGACCTT CGGGACACCT TCGCAGCCAT GGGCCGCCTC 
AATGTGAAGA ATGAGGAGTT GGATGCCATG ATGAAGGAAG CCAGCGGTCC CATCAACTTC 
ACCGTCTTCC TGACCATGTT CGGGGAGAAG CTCAAGGGTG CCGACCCTGA GGATGTGATC 
ACCGGAGCCT TCAAGGTCTT GGACCCTGAG GGAAAGGGCA CCATCAAGAA GAAGTTCCTG 
GAGGAGCTGC TGACCACGCA GTGTGACCGC TTCTCCCAGG AGGAGATCAA GAACATGTGG 
GCGGCCTTCC CCCCCGACGT GGGCGGCAAC GTCGACTACA AAAACATCTG CTACGTCATC 
ACGCACGGCG ACGCCAAGGA CCAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001311388.1
CDS	247..756
Translation	MAPKRAKRRTVEGGSSSVFSMFDQTQIQEFKEAFTVIDQNRDGIIDKEDLRDTFAAMGRLNVKNEELDAMMKEASGPINFTVFLTMFGEKLKGADPEDVITGAFKVLDPEGKGTIKKKFLEELLTTQCDRFSQEEIKNMWAAFPPDVGGNVDYKNICYVITHGDAKDQE

Target ORF information:

RefSeq Version	NM_001324459.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001324459.1

ORF Insert Sequence:

ATGGCACCCA AGAGGGCCAA GAGAAGGACA GTAGAGGGCG GAAGCTCCAG CGTCTTCTCC 
ATGTTCGACC AGACTCAGAT CCAGGAGTTC AAAGAGGCCT TCACTGTGAT CGACCAGAAC 
CGTGATGGTA TTATAGACAA GGAGGACCTT CGGGACACCT TCGCAGCCAT GGGCCGCCTC 
AATGTGAAGA ATGAGGAGTT GGATGCCATG ATGAAGGAAG CCAGCGGTCC CATCAACTTC 
ACCGTCTTCC TGACCATGTT CGGGGAGAAG CTCAAGGGTG CCGACCCTGA GGATGTGATC 
ACCGGAGCCT TCAAGGTCTT GGACCCTGAG GGAAAGGGCA CCATCAAGAA GAAGTTCCTG 
GAGGAGCTGC TGACCACGCA GTGTGACCGC TTCTCCCAGG AGGAGATCAA GAACATGTGG 
GCGGCCTTCC CCCCCGACGT GGGCGGCAAC GTCGACTACA AAAACATCTG CTACGTCATC 
ACGCACGGCG ACGCCAAGGA CCAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYLPF ( NM_013292.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_037424.2 Homo sapiens myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31266
Clone ID Related Accession (Same CDS sequence)	NM_001324458.2 , NM_001324459.1 , NM_013292.4 , NM_013292.3 , NM_013292.5 , NM_001324458.1
Accession Version	NM_013292.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550937600000
Organism	Homo sapiens(human)
Product	myosin regulatory light chain 2, skeletal muscle isoform
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK314082.1 and AA662706.1. On May 5, 2016 this sequence version replaced NM_013292.3. Transcript Variant: This variant (1) is the predominant variant. Variants 1, 2, and 3 all encode the same protein. ##Evidence-Data-START## Transcript exon combination :: CF552525.1, BC012571.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCACCCA AGAGGGCCAA GAGAAGGACA GTAGAGGGCG GAAGCTCCAG CGTCTTCTCC 
ATGTTCGACC AGACTCAGAT CCAGGAGTTC AAAGAGGCCT TCACTGTGAT CGACCAGAAC 
CGTGATGGTA TTATAGACAA GGAGGACCTT CGGGACACCT TCGCAGCCAT GGGCCGCCTC 
AATGTGAAGA ATGAGGAGTT GGATGCCATG ATGAAGGAAG CCAGCGGTCC CATCAACTTC 
ACCGTCTTCC TGACCATGTT CGGGGAGAAG CTCAAGGGTG CCGACCCTGA GGATGTGATC 
ACCGGAGCCT TCAAGGTCTT GGACCCTGAG GGAAAGGGCA CCATCAAGAA GAAGTTCCTG 
GAGGAGCTGC TGACCACGCA GTGTGACCGC TTCTCCCAGG AGGAGATCAA GAACATGTGG 
GCGGCCTTCC CCCCCGACGT GGGCGGCAAC GTCGACTACA AAAACATCTG CTACGTCATC 
ACGCACGGCG ACGCCAAGGA CCAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037424.2
CDS	82..591
Translation	MAPKRAKRRTVEGGSSSVFSMFDQTQIQEFKEAFTVIDQNRDGIIDKEDLRDTFAAMGRLNVKNEELDAMMKEASGPINFTVFLTMFGEKLKGADPEDVITGAFKVLDPEGKGTIKKKFLEELLTTQCDRFSQEEIKNMWAAFPPDVGGNVDYKNICYVITHGDAKDQE

Target ORF information:

RefSeq Version	NM_013292.4
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013292.4

ORF Insert Sequence:

ATGGCACCCA AGAGGGCCAA GAGAAGGACA GTAGAGGGCG GAAGCTCCAG CGTCTTCTCC 
ATGTTCGACC AGACTCAGAT CCAGGAGTTC AAAGAGGCCT TCACTGTGAT CGACCAGAAC 
CGTGATGGTA TTATAGACAA GGAGGACCTT CGGGACACCT TCGCAGCCAT GGGCCGCCTC 
AATGTGAAGA ATGAGGAGTT GGATGCCATG ATGAAGGAAG CCAGCGGTCC CATCAACTTC 
ACCGTCTTCC TGACCATGTT CGGGGAGAAG CTCAAGGGTG CCGACCCTGA GGATGTGATC 
ACCGGAGCCT TCAAGGTCTT GGACCCTGAG GGAAAGGGCA CCATCAAGAA GAAGTTCCTG 
GAGGAGCTGC TGACCACGCA GTGTGACCGC TTCTCCCAGG AGGAGATCAA GAACATGTGG 
GCGGCCTTCC CCCCCGACGT GGGCGGCAAC GTCGACTACA AAAACATCTG CTACGTCATC 
ACGCACGGCG ACGCCAAGGA CCAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYLPF ( NM_013292.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_037424.2 Homo sapiens myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF), mRNA.

Price & Availability↑

CloneID	OHu31266
Clone ID Related Accession (Same CDS sequence)	NM_001324458.2 , NM_001324459.1 , NM_013292.4 , NM_013292.3 , NM_013292.5 , NM_001324458.1
Accession Version	NM_013292.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426262400000
Organism	Homo sapiens(Human)
Product	myosin regulatory light chain 2, skeletal muscle isoform
Comment

ATGGCACCCA AGAGGGCCAA GAGAAGGACA GTAGAGGGCG GAAGCTCCAG CGTCTTCTCC 
ATGTTCGACC AGACTCAGAT CCAGGAGTTC AAAGAGGCCT TCACTGTGAT CGACCAGAAC 
CGTGATGGTA TTATAGACAA GGAGGACCTT CGGGACACCT TCGCAGCCAT GGGCCGCCTC 
AATGTGAAGA ATGAGGAGTT GGATGCCATG ATGAAGGAAG CCAGCGGTCC CATCAACTTC 
ACCGTCTTCC TGACCATGTT CGGGGAGAAG CTCAAGGGTG CCGACCCTGA GGATGTGATC 
ACCGGAGCCT TCAAGGTCTT GGACCCTGAG GGAAAGGGCA CCATCAAGAA GAAGTTCCTG 
GAGGAGCTGC TGACCACGCA GTGTGACCGC TTCTCCCAGG AGGAGATCAA GAACATGTGG 
GCGGCCTTCC CCCCCGACGT GGGCGGCAAC GTCGACTACA AAAACATCTG CTACGTCATC 
ACGCACGGCG ACGCCAAGGA CCAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037424.2
Translation	MAPKRAKRRTVEGGSSSVFSMFDQTQIQEFKEAFTVIDQNRDGIIDKEDLRDTFAAMGRLNVKNEELDAMMKEASGPINFTVFLTMFGEKLKGADPEDVITGAFKVLDPEGKGTIKKKFLEELLTTQCDRFSQEEIKNMWAAFPPDVGGNVDYKNICYVITHGDAKDQE

Target ORF information:

RefSeq Version	NM_013292.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013292.3

ORF Insert Sequence:

ATGGCACCCA AGAGGGCCAA GAGAAGGACA GTAGAGGGCG GAAGCTCCAG CGTCTTCTCC 
ATGTTCGACC AGACTCAGAT CCAGGAGTTC AAAGAGGCCT TCACTGTGAT CGACCAGAAC 
CGTGATGGTA TTATAGACAA GGAGGACCTT CGGGACACCT TCGCAGCCAT GGGCCGCCTC 
AATGTGAAGA ATGAGGAGTT GGATGCCATG ATGAAGGAAG CCAGCGGTCC CATCAACTTC 
ACCGTCTTCC TGACCATGTT CGGGGAGAAG CTCAAGGGTG CCGACCCTGA GGATGTGATC 
ACCGGAGCCT TCAAGGTCTT GGACCCTGAG GGAAAGGGCA CCATCAAGAA GAAGTTCCTG 
GAGGAGCTGC TGACCACGCA GTGTGACCGC TTCTCCCAGG AGGAGATCAA GAACATGTGG 
GCGGCCTTCC CCCCCGACGT GGGCGGCAAC GTCGACTACA AAAACATCTG CTACGTCATC 
ACGCACGGCG ACGCCAAGGA CCAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYLPF ( NM_013292.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_037424.2 Homo sapiens myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31266
Clone ID Related Accession (Same CDS sequence)	NM_001324458.2 , NM_001324459.1 , NM_013292.4 , NM_013292.3 , NM_013292.5 , NM_001324458.1
Accession Version	NM_013292.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577721600000
Organism	Homo sapiens(human)
Product	myosin regulatory light chain 2, skeletal muscle isoform
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK314082.1 and AA662706.1. On May 17, 2019 this sequence version replaced NM_013292.4. Transcript Variant: This variant (1) is the predominant variant. Variants 1, 2, and 3 all encode the same protein. ##Evidence-Data-START## Transcript exon combination :: DRR138525.680494.1, BF693409.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000322861.12/ ENSP00000325239.7 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCACCCA AGAGGGCCAA GAGAAGGACA GTAGAGGGCG GAAGCTCCAG CGTCTTCTCC 
ATGTTCGACC AGACTCAGAT CCAGGAGTTC AAAGAGGCCT TCACTGTGAT CGACCAGAAC 
CGTGATGGTA TTATAGACAA GGAGGACCTT CGGGACACCT TCGCAGCCAT GGGCCGCCTC 
AATGTGAAGA ATGAGGAGTT GGATGCCATG ATGAAGGAAG CCAGCGGTCC CATCAACTTC 
ACCGTCTTCC TGACCATGTT CGGGGAGAAG CTCAAGGGTG CCGACCCTGA GGATGTGATC 
ACCGGAGCCT TCAAGGTCTT GGACCCTGAG GGAAAGGGCA CCATCAAGAA GAAGTTCCTG 
GAGGAGCTGC TGACCACGCA GTGTGACCGC TTCTCCCAGG AGGAGATCAA GAACATGTGG 
GCGGCCTTCC CCCCCGACGT GGGCGGCAAC GTCGACTACA AAAACATCTG CTACGTCATC 
ACGCACGGCG ACGCCAAGGA CCAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037424.2
CDS	57..566
Translation	MAPKRAKRRTVEGGSSSVFSMFDQTQIQEFKEAFTVIDQNRDGIIDKEDLRDTFAAMGRLNVKNEELDAMMKEASGPINFTVFLTMFGEKLKGADPEDVITGAFKVLDPEGKGTIKKKFLEELLTTQCDRFSQEEIKNMWAAFPPDVGGNVDYKNICYVITHGDAKDQE

Target ORF information:

RefSeq Version	NM_013292.5
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013292.5

ORF Insert Sequence:

ATGGCACCCA AGAGGGCCAA GAGAAGGACA GTAGAGGGCG GAAGCTCCAG CGTCTTCTCC 
ATGTTCGACC AGACTCAGAT CCAGGAGTTC AAAGAGGCCT TCACTGTGAT CGACCAGAAC 
CGTGATGGTA TTATAGACAA GGAGGACCTT CGGGACACCT TCGCAGCCAT GGGCCGCCTC 
AATGTGAAGA ATGAGGAGTT GGATGCCATG ATGAAGGAAG CCAGCGGTCC CATCAACTTC 
ACCGTCTTCC TGACCATGTT CGGGGAGAAG CTCAAGGGTG CCGACCCTGA GGATGTGATC 
ACCGGAGCCT TCAAGGTCTT GGACCCTGAG GGAAAGGGCA CCATCAAGAA GAAGTTCCTG 
GAGGAGCTGC TGACCACGCA GTGTGACCGC TTCTCCCAGG AGGAGATCAA GAACATGTGG 
GCGGCCTTCC CCCCCGACGT GGGCGGCAAC GTCGACTACA AAAACATCTG CTACGTCATC 
ACGCACGGCG ACGCCAAGGA CCAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYLPF ( NM_001324458.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001311387.1 Homo sapiens myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF), transcript variant 2, mRNA.

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CloneID	OHu31266
Clone ID Related Accession (Same CDS sequence)	NM_001324458.2 , NM_001324459.1 , NM_013292.4 , NM_013292.3 , NM_013292.5 , NM_001324458.1
Accession Version	NM_001324458.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550937600000
Organism	Homo sapiens(human)
Product	myosin regulatory light chain 2, skeletal muscle isoform
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BI837593.1, BC012571.1 and AA662706.1. Transcript Variant: This variant (2) and variants 1 and 3 all encode the same protein. ##Evidence-Data-START## Transcript exon combination :: BI837593.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCACCCA AGAGGGCCAA GAGAAGGACA GTAGAGGGCG GAAGCTCCAG CGTCTTCTCC 
ATGTTCGACC AGACTCAGAT CCAGGAGTTC AAAGAGGCCT TCACTGTGAT CGACCAGAAC 
CGTGATGGTA TTATAGACAA GGAGGACCTT CGGGACACCT TCGCAGCCAT GGGCCGCCTC 
AATGTGAAGA ATGAGGAGTT GGATGCCATG ATGAAGGAAG CCAGCGGTCC CATCAACTTC 
ACCGTCTTCC TGACCATGTT CGGGGAGAAG CTCAAGGGTG CCGACCCTGA GGATGTGATC 
ACCGGAGCCT TCAAGGTCTT GGACCCTGAG GGAAAGGGCA CCATCAAGAA GAAGTTCCTG 
GAGGAGCTGC TGACCACGCA GTGTGACCGC TTCTCCCAGG AGGAGATCAA GAACATGTGG 
GCGGCCTTCC CCCCCGACGT GGGCGGCAAC GTCGACTACA AAAACATCTG CTACGTCATC 
ACGCACGGCG ACGCCAAGGA CCAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001311387.1
CDS	219..728
Translation	MAPKRAKRRTVEGGSSSVFSMFDQTQIQEFKEAFTVIDQNRDGIIDKEDLRDTFAAMGRLNVKNEELDAMMKEASGPINFTVFLTMFGEKLKGADPEDVITGAFKVLDPEGKGTIKKKFLEELLTTQCDRFSQEEIKNMWAAFPPDVGGNVDYKNICYVITHGDAKDQE

Target ORF information:

RefSeq Version	NM_001324458.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001324458.1

ORF Insert Sequence:

ATGGCACCCA AGAGGGCCAA GAGAAGGACA GTAGAGGGCG GAAGCTCCAG CGTCTTCTCC 
ATGTTCGACC AGACTCAGAT CCAGGAGTTC AAAGAGGCCT TCACTGTGAT CGACCAGAAC 
CGTGATGGTA TTATAGACAA GGAGGACCTT CGGGACACCT TCGCAGCCAT GGGCCGCCTC 
AATGTGAAGA ATGAGGAGTT GGATGCCATG ATGAAGGAAG CCAGCGGTCC CATCAACTTC 
ACCGTCTTCC TGACCATGTT CGGGGAGAAG CTCAAGGGTG CCGACCCTGA GGATGTGATC 
ACCGGAGCCT TCAAGGTCTT GGACCCTGAG GGAAAGGGCA CCATCAAGAA GAAGTTCCTG 
GAGGAGCTGC TGACCACGCA GTGTGACCGC TTCTCCCAGG AGGAGATCAA GAACATGTGG 
GCGGCCTTCC CCCCCGACGT GGGCGGCAAC GTCGACTACA AAAACATCTG CTACGTCATC 
ACGCACGGCG ACGCCAAGGA CCAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of myosin II regulatory light chain isoforms in HeLa cells.
Cytoskeleton (Hoboken, N.J.)72(12)609-20(2015 Dec)
Kondo T,Okada M,Kunihiro K,Takahashi M,Yaoita Y,Hosoya H,Hamao K

Human fast skeletal myosin light chain 2 cDNA: isolation, tissue specific expression of the single copy gene, comparative sequence analysis of isoforms and evolutionary relationships.
DNA sequence : the journal of DNA sequencing and mapping14(5)339-50(2003 Oct)
Sachdev S,Raychowdhury MK,Sarkar S

The nucleotide sequence of a rat myosin light chain 2 gene.
Nucleic acids research12(18)7175-86(1984 Sep)
Nudel U,Calvo JM,Shani M,Levy Z

Cloning and characterization of cDNA sequences corresponding to myosin light chains 1, 2, and 3, troponin-C, troponin-T, alpha-tropomyosin, and alpha-actin.
The Journal of biological chemistry257(18)11078-86(1982 Sep)
Garfinkel LI,Periasamy M,Nadal-Ginard B

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MYLPF cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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