MRPL15 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MRPL15
Entrez Gene ID	29088
Full Name	mitochondrial ribosomal protein L15
Synonyms	HSPC145,L15mt,MRP-L15,MRP-L7,RPML7
General protein information	Preferred Names mitochondrial ribosomal protein L15 Names 39S ribosomal protein L15, mitochondrial mitochondrial large ribosomal subunit protein uL15m
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8q11.23
Summary	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL15 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 15q. [provided by RefSeq, Jul 2008].
Disorder MIM:	611828

mRNA and Protein(s)

mRNA	Protein	Name
NM_014175.3	NP_054894.1	39S ribosomal protein L15, mitochondrial
NM_014175.4	NP_054894.1	39S ribosomal protein L15, mitochondrial
XM_017013362.1	XP_016868851.1	39S ribosomal protein L15, mitochondrial isoform X1
XM_017013362.2	XP_016868851.1	39S ribosomal protein L15, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011946	XP_320585.1
Bos taurus (cattle)	MRPL15	NP_001068913.1
Caenorhabditis elegans (roundworm)	mrpl-15	NP_490854.1
Macaca mulatta (Rhesus monkey)	MRPL15	XP_001082976.1
Danio rerio (zebrafish)	mrpl15	NP_001003435.1
Mus musculus (house mouse)	Mrpl15	NP_001171129.1
Gallus gallus (chicken)	MRPL15	NP_001006388.1
Homo sapiens (human)	MRPL15	NP_054894.1
Canis lupus familiaris (dog)	MRPL15	XP_003640068.1
Pan troglodytes (chimpanzee)	MRPL15	XP_528136.1
Drosophila melanogaster (fruit fly)	mRpL15	NP_524185.1
Xenopus tropicalis (tropical clawed frog)	mrpl15	NP_001120107.1

Gene Ontology
Bibliography

Related articles in PubMed

Structures of the human mitochondrial ribosome in native states of assembly.
Brown A, Rathore S, Kimanius D, Aibara S, Bai XC, Rorbach J, Amunts A, Ramakrishnan V
Nature structural & molecular biology24(10)866-869(2017 Oct)

Structure of the large ribosomal subunit from human mitochondria.
Brown A, Amunts A, Bai XC, Sugimoto Y, Edwards PC, Murshudov G, Scheres SHW, Ramakrishnan V
Science (New York, N.Y.)346(6210)718-722(2014 Nov)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanhäusser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Next-generation sequencing to generate interactome datasets.
Yu H, Tardivo L, Tam S, Weiner E, Gebreab F, Fan C, Svrzikapa N, Hirozane-Kishikawa T, Rietman E, Yang X, Sahalie J, Salehi-Ashtiani K, Hao T, Cusick ME, Hill DE, Roth FP, Braun P, Vidal M
Nature methods8(6)478-80(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MRPL15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MRPL15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu97932	XM_017013362.2 Latest version!	Homo sapiens mitochondrial ribosomal protein L15 (MRPL15), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu30650	NM_014175.4 Latest version!	Homo sapiens mitochondrial ribosomal protein L15 (MRPL15), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$216.30 $309.00
View Old Accession Versions >>
OHu30650	NM_014175.3	Homo sapiens mitochondrial ribosomal protein L15 (MRPL15), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$216.30 $309.00
OHu97932	XM_017013362.1	Homo sapiens mitochondrial ribosomal protein L15 (MRPL15), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MRPL15 ( XM_017013362.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868851.1 Homo sapiens mitochondrial ribosomal protein L15 (MRPL15), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu97932
Clone ID Related Accession (Same CDS sequence)	XM_017013362.1 , XM_017013362.2
Accession Version	XM_017013362.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	39S ribosomal protein L15, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGTC CCTTGCAGGG CGGTGGGGCC CGGGCCCTGG ACCTACTCCG GGGCCTGCCG 
CGTGTGAGCC TGGCCAACTT AAAGCCGAAT CCCGGCTCCA AGAAACCGGA GAGAAGACCA 
AGAGGTCGGA GAAGAGGTAG AAAATGTGGC AGAGGCCATA AAGGAGAAAG GCAAAGAGGA 
ACCCGGCCCC GCTTGGGCTT TGAGGGAGGC CAGACTCCAT TTTACATCCG AATCCCAAAA 
TACGGGTTTA ACGAAGGACA TAGTTTCAGA CGCCAGTATA AGCCTTTGAG TCTCAATAGA 
CTGCAGTATC TTATTGATTT GGGTCGTGTT GATCCTAGTC AACCTATTGA CTTAACCCAG 
CTTGTCAATG GGAGAGGTGT GACCATCCAG CCACTTAAAA GGGATTATGG TGTCCAGCTG 
GTTGAGGAGG GTGCTGACAC CTTTACGGCA AAAGTTAATA TTGAAGTACA GTTGGCTTCA 
GAACTAGCTA TTGCTGCCAT TGAAAAAAAT GGTGGTGTTG TTACTACAGC CTTCTATGAT 
CCAAGAAGTC TGGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868851.1
CDS	75..632
Translation	MAGPLQGGGARALDLLRGLPRVSLANLKPNPGSKKPERRPRGRRRGRKCGRGHKGERQRGTRPRLGFEGGQTPFYIRIPKYGFNEGHSFRRQYKPLSLNRLQYLIDLGRVDPSQPIDLTQLVNGRGVTIQPLKRDYGVQLVEEGADTFTAKVNIEVQLASELAIAAIEKNGGVVTTAFYDPRSLG

Target ORF information:

RefSeq Version	XM_017013362.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L15 (MRPL15), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013362.1

ORF Insert Sequence:

ATGGCCGGTC CCTTGCAGGG CGGTGGGGCC CGGGCCCTGG ACCTACTCCG GGGCCTGCCG 
CGTGTGAGCC TGGCCAACTT AAAGCCGAAT CCCGGCTCCA AGAAACCGGA GAGAAGACCA 
AGAGGTCGGA GAAGAGGTAG AAAATGTGGC AGAGGCCATA AAGGAGAAAG GCAAAGAGGA 
ACCCGGCCCC GCTTGGGCTT TGAGGGAGGC CAGACTCCAT TTTACATCCG AATCCCAAAA 
TACGGGTTTA ACGAAGGACA TAGTTTCAGA CGCCAGTATA AGCCTTTGAG TCTCAATAGA 
CTGCAGTATC TTATTGATTT GGGTCGTGTT GATCCTAGTC AACCTATTGA CTTAACCCAG 
CTTGTCAATG GGAGAGGTGT GACCATCCAG CCACTTAAAA GGGATTATGG TGTCCAGCTG 
GTTGAGGAGG GTGCTGACAC CTTTACGGCA AAAGTTAATA TTGAAGTACA GTTGGCTTCA 
GAACTAGCTA TTGCTGCCAT TGAAAAAAAT GGTGGTGTTG TTACTACAGC CTTCTATGAT 
CCAAGAAGTC TGGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPL15 ( XM_017013362.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868851.1 Homo sapiens mitochondrial ribosomal protein L15 (MRPL15), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu97932
Clone ID Related Accession (Same CDS sequence)	XM_017013362.1 , XM_017013362.2
Accession Version	XM_017013362.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	39S ribosomal protein L15, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017013362.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGTC CCTTGCAGGG CGGTGGGGCC CGGGCCCTGG ACCTACTCCG GGGCCTGCCG 
CGTGTGAGCC TGGCCAACTT AAAGCCGAAT CCCGGCTCCA AGAAACCGGA GAGAAGACCA 
AGAGGTCGGA GAAGAGGTAG AAAATGTGGC AGAGGCCATA AAGGAGAAAG GCAAAGAGGA 
ACCCGGCCCC GCTTGGGCTT TGAGGGAGGC CAGACTCCAT TTTACATCCG AATCCCAAAA 
TACGGGTTTA ACGAAGGACA TAGTTTCAGA CGCCAGTATA AGCCTTTGAG TCTCAATAGA 
CTGCAGTATC TTATTGATTT GGGTCGTGTT GATCCTAGTC AACCTATTGA CTTAACCCAG 
CTTGTCAATG GGAGAGGTGT GACCATCCAG CCACTTAAAA GGGATTATGG TGTCCAGCTG 
GTTGAGGAGG GTGCTGACAC CTTTACGGCA AAAGTTAATA TTGAAGTACA GTTGGCTTCA 
GAACTAGCTA TTGCTGCCAT TGAAAAAAAT GGTGGTGTTG TTACTACAGC CTTCTATGAT 
CCAAGAAGTC TGGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868851.1
CDS	61..618
Translation	MAGPLQGGGARALDLLRGLPRVSLANLKPNPGSKKPERRPRGRRRGRKCGRGHKGERQRGTRPRLGFEGGQTPFYIRIPKYGFNEGHSFRRQYKPLSLNRLQYLIDLGRVDPSQPIDLTQLVNGRGVTIQPLKRDYGVQLVEEGADTFTAKVNIEVQLASELAIAAIEKNGGVVTTAFYDPRSLG

Target ORF information:

RefSeq Version	XM_017013362.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L15 (MRPL15), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013362.2

ORF Insert Sequence:

ATGGCCGGTC CCTTGCAGGG CGGTGGGGCC CGGGCCCTGG ACCTACTCCG GGGCCTGCCG 
CGTGTGAGCC TGGCCAACTT AAAGCCGAAT CCCGGCTCCA AGAAACCGGA GAGAAGACCA 
AGAGGTCGGA GAAGAGGTAG AAAATGTGGC AGAGGCCATA AAGGAGAAAG GCAAAGAGGA 
ACCCGGCCCC GCTTGGGCTT TGAGGGAGGC CAGACTCCAT TTTACATCCG AATCCCAAAA 
TACGGGTTTA ACGAAGGACA TAGTTTCAGA CGCCAGTATA AGCCTTTGAG TCTCAATAGA 
CTGCAGTATC TTATTGATTT GGGTCGTGTT GATCCTAGTC AACCTATTGA CTTAACCCAG 
CTTGTCAATG GGAGAGGTGT GACCATCCAG CCACTTAAAA GGGATTATGG TGTCCAGCTG 
GTTGAGGAGG GTGCTGACAC CTTTACGGCA AAAGTTAATA TTGAAGTACA GTTGGCTTCA 
GAACTAGCTA TTGCTGCCAT TGAAAAAAAT GGTGGTGTTG TTACTACAGC CTTCTATGAT 
CCAAGAAGTC TGGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPL15 ( NM_014175.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_054894.1 Homo sapiens mitochondrial ribosomal protein L15 (MRPL15), mRNA.

Price & Availability↑

CloneID	OHu30650
Clone ID Related Accession (Same CDS sequence)	NM_014175.3 , NM_014175.4
Accession Version	NM_014175.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1533830400000
Organism	Homo sapiens(human)
Product	39S ribosomal protein L15, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC000891.1, DA504537.1 and BM510276.1. On Mar 5, 2008 this sequence version replaced NM_014175.2. ##Evidence-Data-START## Transcript exon combination :: BC000891.1, SRR1660803.16072.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCGGTC CCTTGCAGGG CGGTGGGGCC CGGGCCCTGG ACCTACTCCG GGGCCTGCCG 
CGTGTGAGCC TGGCCAACTT AAAGCCGAAT CCCGGCTCCA AGAAACCGGA GAGAAGACCA 
AGAGGTCGGA GAAGAGGTAG AAAATGTGGC AGAGGCCATA AAGGAGAAAG GCAAAGAGGA 
ACCCGGCCCC GCTTGGGCTT TGAGGGAGGC CAGACTCCAT TTTACATCCG AATCCCAAAA 
TACGGGTTTA ACGAAGGACA TAGTTTCAGA CGCCAGTATA AGCCTTTGAG TCTCAATAGA 
CTGCAGTATC TTATTGATTT GGGTCGTGTT GATCCTAGTC AACCTATTGA CTTAACCCAG 
CTTGTCAATG GGAGAGGTGT GACCATCCAG CCACTTAAAA GGGATTATGG TGTCCAGCTG 
GTTGAGGAGG GTGCTGACAC CTTTACGGCA AAAGTTAATA TTGAAGTACA GTTGGCTTCA 
GAACTAGCTA TTGCTGCCAT TGAAAAAAAT GGTGGTGTTG TTACTACAGC CTTCTATGAT 
CCAAGAAGTC TGGACATTGT ATGCAAACCT GTTCCATTCT TTCTTCGTGG ACAACCCATT 
CCAAAAAGAA TGCTTCCACC AGAAGAACTG GTACCATATT ACACTGATGC AAAGAACCGT 
GGGTACCTGG CGGATCCTGC CAAATTTCCT GAAGCACGAC TTGAACTCGC CAGGAAGTAT 
GGTTATATCT TACCTGATAT CACTAAAGAT GAACTCTTCA AAATGCTCTG TACTAGGAAG 
GATCCAAGGC AGATTTTCTT TGGTCTTGCT CCAGGATGGG TGGTGAATAT GGCCGATAAG 
AAAATCCTAA AACCTACAGA TGAAAATCTC CTTAAGTATT ATACCTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054894.1
CDS	64..954
Translation	MAGPLQGGGARALDLLRGLPRVSLANLKPNPGSKKPERRPRGRRRGRKCGRGHKGERQRGTRPRLGFEGGQTPFYIRIPKYGFNEGHSFRRQYKPLSLNRLQYLIDLGRVDPSQPIDLTQLVNGRGVTIQPLKRDYGVQLVEEGADTFTAKVNIEVQLASELAIAAIEKNGGVVTTAFYDPRSLDIVCKPVPFFLRGQPIPKRMLPPEELVPYYTDAKNRGYLADPAKFPEARLELARKYGYILPDITKDELFKMLCTRKDPRQIFFGLAPGWVVNMADKKILKPTDENLLKYYTS

Target ORF information:

RefSeq Version	NM_014175.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L15 (MRPL15), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014175.3

ORF Insert Sequence:

ATGGCCGGTC CCTTGCAGGG CGGTGGGGCC CGGGCCCTGG ACCTACTCCG GGGCCTGCCG 
CGTGTGAGCC TGGCCAACTT AAAGCCGAAT CCCGGCTCCA AGAAACCGGA GAGAAGACCA 
AGAGGTCGGA GAAGAGGTAG AAAATGTGGC AGAGGCCATA AAGGAGAAAG GCAAAGAGGA 
ACCCGGCCCC GCTTGGGCTT TGAGGGAGGC CAGACTCCAT TTTACATCCG AATCCCAAAA 
TACGGGTTTA ACGAAGGACA TAGTTTCAGA CGCCAGTATA AGCCTTTGAG TCTCAATAGA 
CTGCAGTATC TTATTGATTT GGGTCGTGTT GATCCTAGTC AACCTATTGA CTTAACCCAG 
CTTGTCAATG GGAGAGGTGT GACCATCCAG CCACTTAAAA GGGATTATGG TGTCCAGCTG 
GTTGAGGAGG GTGCTGACAC CTTTACGGCA AAAGTTAATA TTGAAGTACA GTTGGCTTCA 
GAACTAGCTA TTGCTGCCAT TGAAAAAAAT GGTGGTGTTG TTACTACAGC CTTCTATGAT 
CCAAGAAGTC TGGACATTGT ATGCAAACCT GTTCCATTCT TTCTTCGTGG ACAACCCATT 
CCAAAAAGAA TGCTTCCACC AGAAGAACTG GTACCATATT ACACTGATGC AAAGAACCGT 
GGGTACCTGG CGGATCCTGC CAAATTTCCT GAAGCACGAC TTGAACTCGC CAGGAAGTAT 
GGTTATATCT TACCTGATAT CACTAAAGAT GAACTCTTCA AAATGCTCTG TACTAGGAAG 
GATCCAAGGC AGATTTTCTT TGGTCTTGCT CCAGGATGGG TGGTGAATAT GGCCGATAAG 
AAAATCCTAA AACCTACAGA TGAAAATCTC CTTAAGTATT ATACCTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPL15 ( NM_014175.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_054894.1 Homo sapiens mitochondrial ribosomal protein L15 (MRPL15), mRNA.

Price & Availability↑

CloneID	OHu30650
Clone ID Related Accession (Same CDS sequence)	NM_014175.3 , NM_014175.4
Accession Version	NM_014175.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571932800000
Organism	Homo sapiens(human)
Product	39S ribosomal protein L15, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC000891.1, DA504537.1 and BM510276.1. On May 17, 2019 this sequence version replaced NM_014175.3. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.12147.1, SRR1660807.85521.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000260102.9/ ENSP00000260102.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCGGTC CCTTGCAGGG CGGTGGGGCC CGGGCCCTGG ACCTACTCCG GGGCCTGCCG 
CGTGTGAGCC TGGCCAACTT AAAGCCGAAT CCCGGCTCCA AGAAACCGGA GAGAAGACCA 
AGAGGTCGGA GAAGAGGTAG AAAATGTGGC AGAGGCCATA AAGGAGAAAG GCAAAGAGGA 
ACCCGGCCCC GCTTGGGCTT TGAGGGAGGC CAGACTCCAT TTTACATCCG AATCCCAAAA 
TACGGGTTTA ACGAAGGACA TAGTTTCAGA CGCCAGTATA AGCCTTTGAG TCTCAATAGA 
CTGCAGTATC TTATTGATTT GGGTCGTGTT GATCCTAGTC AACCTATTGA CTTAACCCAG 
CTTGTCAATG GGAGAGGTGT GACCATCCAG CCACTTAAAA GGGATTATGG TGTCCAGCTG 
GTTGAGGAGG GTGCTGACAC CTTTACGGCA AAAGTTAATA TTGAAGTACA GTTGGCTTCA 
GAACTAGCTA TTGCTGCCAT TGAAAAAAAT GGTGGTGTTG TTACTACAGC CTTCTATGAT 
CCAAGAAGTC TGGACATTGT ATGCAAACCT GTTCCATTCT TTCTTCGTGG ACAACCCATT 
CCAAAAAGAA TGCTTCCACC AGAAGAACTG GTACCATATT ACACTGATGC AAAGAACCGT 
GGGTACCTGG CGGATCCTGC CAAATTTCCT GAAGCACGAC TTGAACTCGC CAGGAAGTAT 
GGTTATATCT TACCTGATAT CACTAAAGAT GAACTCTTCA AAATGCTCTG TACTAGGAAG 
GATCCAAGGC AGATTTTCTT TGGTCTTGCT CCAGGATGGG TGGTGAATAT GGCCGATAAG 
AAAATCCTAA AACCTACAGA TGAAAATCTC CTTAAGTATT ATACCTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054894.1
CDS	44..934
Translation	MAGPLQGGGARALDLLRGLPRVSLANLKPNPGSKKPERRPRGRRRGRKCGRGHKGERQRGTRPRLGFEGGQTPFYIRIPKYGFNEGHSFRRQYKPLSLNRLQYLIDLGRVDPSQPIDLTQLVNGRGVTIQPLKRDYGVQLVEEGADTFTAKVNIEVQLASELAIAAIEKNGGVVTTAFYDPRSLDIVCKPVPFFLRGQPIPKRMLPPEELVPYYTDAKNRGYLADPAKFPEARLELARKYGYILPDITKDELFKMLCTRKDPRQIFFGLAPGWVVNMADKKILKPTDENLLKYYTS

Target ORF information:

RefSeq Version	NM_014175.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L15 (MRPL15), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014175.4

ORF Insert Sequence:

ATGGCCGGTC CCTTGCAGGG CGGTGGGGCC CGGGCCCTGG ACCTACTCCG GGGCCTGCCG 
CGTGTGAGCC TGGCCAACTT AAAGCCGAAT CCCGGCTCCA AGAAACCGGA GAGAAGACCA 
AGAGGTCGGA GAAGAGGTAG AAAATGTGGC AGAGGCCATA AAGGAGAAAG GCAAAGAGGA 
ACCCGGCCCC GCTTGGGCTT TGAGGGAGGC CAGACTCCAT TTTACATCCG AATCCCAAAA 
TACGGGTTTA ACGAAGGACA TAGTTTCAGA CGCCAGTATA AGCCTTTGAG TCTCAATAGA 
CTGCAGTATC TTATTGATTT GGGTCGTGTT GATCCTAGTC AACCTATTGA CTTAACCCAG 
CTTGTCAATG GGAGAGGTGT GACCATCCAG CCACTTAAAA GGGATTATGG TGTCCAGCTG 
GTTGAGGAGG GTGCTGACAC CTTTACGGCA AAAGTTAATA TTGAAGTACA GTTGGCTTCA 
GAACTAGCTA TTGCTGCCAT TGAAAAAAAT GGTGGTGTTG TTACTACAGC CTTCTATGAT 
CCAAGAAGTC TGGACATTGT ATGCAAACCT GTTCCATTCT TTCTTCGTGG ACAACCCATT 
CCAAAAAGAA TGCTTCCACC AGAAGAACTG GTACCATATT ACACTGATGC AAAGAACCGT 
GGGTACCTGG CGGATCCTGC CAAATTTCCT GAAGCACGAC TTGAACTCGC CAGGAAGTAT 
GGTTATATCT TACCTGATAT CACTAAAGAT GAACTCTTCA AAATGCTCTG TACTAGGAAG 
GATCCAAGGC AGATTTTCTT TGGTCTTGCT CCAGGATGGG TGGTGAATAT GGCCGATAAG 
AAAATCCTAA AACCTACAGA TGAAAATCTC CTTAAGTATT ATACCTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structures of the human mitochondrial ribosome in native states of assembly.
Nature structural & molecular biology24(10)866-869(2017 Oct)
Brown A,Rathore S,Kimanius D,Aibara S,Bai XC,Rorbach J,Amunts A,Ramakrishnan V

Structure of the large ribosomal subunit from human mitochondria.
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MRPL15 cDNA ORF clone, Homo sapiens(human)

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