BHLHE22 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	BHLHE22
Entrez Gene ID	27319
Full Name	basic helix-loop-helix family member e22
Synonyms	BHLHB5,Beta3,Beta3a,CAGL85,TNRC20
General protein information	Preferred Names basic helix-loop-helix family member e22 Names class E basic helix-loop-helix protein 22 basic helix-loop-helix domain containing, class B, 5 class B basic helix-loop-helix protein 5 trinucleotide repeat containing 20
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8q12.3
Summary	This gene encodes a protein that belongs to the basic helix-loop-helix (bHLH) family of transcription factors that regulate cell fate determination, proliferation, and differentiation. A similar protein in mouse is required for the development of the dorsal cochlear nuclei, and is thought to play a role in in the differentiation of neurons involved in sensory input. The mouse protein also functions in retinogenesis. [provided by RefSeq, Oct 2016]. COMPLETENESS: complete on the 3' end.
Disorder MIM:	613483

mRNA and Protein(s)

mRNA	Protein	Name
NM_152414.4	NP_689627.1	class E basic helix-loop-helix protein 22
NM_152414.5	NP_689627.1	class E basic helix-loop-helix protein 22

Pathways from BioSystems

Homologies

Homo sapiens (human)	BHLHE22	NP_689627.1
Macaca mulatta (Rhesus monkey)	BHLHE22	XP_001092364.1
Xenopus tropicalis (tropical clawed frog)	bhlhe22	NP_001072933.1
Bos taurus (cattle)	LOC101902844	XP_005215555.1
Mus musculus (house mouse)	Bhlhe22	NP_067535.3
Rattus norvegicus (Norway rat)	Bhlhe22	NP_001102410.1
Danio rerio (zebrafish)	bhlhe22	NP_957249.2
Pan troglodytes (chimpanzee)	BHLHE22	XP_003951318.1

Gene Ontology
Bibliography

Related articles in PubMed

A census of human transcription factors: function, expression and evolution.
Vaquerizas JM, Kummerfeld SK, Teichmann SA, Luscombe NM
Nature reviews. Genetics10(4)252-63(2009 04)

Phylogenetic and expression analysis of the basic helix-loop-helix transcription factor gene family: genomic approach to cellular differentiation.
Stevens JD, Roalson EH, Skinner MK
Differentiation; research in biological diversity76(9)1006-22(2008 Nov)

Exhaustive identification of human class II basic helix-loop-helix proteins by virtual library screening.
McLellan AS, Langlands K, Kealey T
Mechanisms of development119 Suppl 1S285-91(2002 Dec)

Exhaustive identification of human class II basic helix-loop-helix proteins by virtual library screening.
McLellan AS, Langlands K, Kealey T
Gene expression patterns : GEP2(3-4)329-35(2002 Dec)

Functional and structural characterization of the human gene BHLHB5, encoding a basic helix-loop-helix transcription factor.
Xu ZP, Dutra A, Stellrecht CM, Wu C, Piatigorsky J, Saunders GF
Genomics80(3)311-8(2002 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BHLHE22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BHLHE22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu08658	NM_152414.5 Latest version!	Homo sapiens basic helix-loop-helix family member e22 (BHLHE22), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
View Old Accession Versions >>
OHu08658	NM_152414.4	Homo sapiens basic helix-loop-helix family member e22 (BHLHE22), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BHLHE22 ( NM_152414.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_689627.1 Homo sapiens basic helix-loop-helix family member e22 (BHLHE22), mRNA.

Price & Availability↑

CloneID	OHu08658
Clone ID Related Accession (Same CDS sequence)	NM_152414.4 , NM_152414.5
Accession Version	NM_152414.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1529769600000
Organism	Homo sapiens(human)
Product	class E basic helix-loop-helix protein 22
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI867692.1, AA927689.1, AK293979.1, BK000273.1, AK091333.1, AC090136.6 and AW206610.1. This sequence is a reference standard in the RefSeqGene project. On Jan 21, 2011 this sequence version replaced NM_152414.3. COMPLETENESS: complete on the 3' end.

ATGGAGCGCG GGATGCACCT CGGTGCAGCG GCCGCCGGCG AGGACGACCT CTTCCTGCAC 
AAGAGCCTGA GCGCCTCCAC CTCCAAGCGC TTGGAAGCGG CTTTCCGCTC CACGCCCCCG 
GGCATGGACC TGTCCCTGGC GCCGCCGCCT CGGGAACGCC CGGCGTCCTC CTCCTCGTCG 
CCCCTGGGCT GCTTCGAGCC GGCTGACCCC GAGGGGGCAG GGCTGCTGTT GCCGCCGCCT 
GGAGGAGGCG GCGGCGGCAG CGCGGGAAGT GGCGGCGGCG GCGGCGGCGG GGTGGGTGTC 
CCCGGGCTGC TAGTAGGTTC AGCCGGCGTT GGGGGCGACC CTAGCCTAAG CAGCCTGCCG 
GCCGGGGCCG CCCTTTGCCT CAAGTACGGC GAAAGCGCGA GCCGGGGCTC GGTGGCCGAG 
AGCAGCGGCG GCGAGCAGAG CCCCGACGAC GACAGCGACG GTCGCTGCGA GCTCGTGCTG 
CGGGCCGGAG TAGCCGACCC GCGGGCCTCC CCGGGAGCGG GAGGTGGTGG CGCGAAGGCA 
GCCGAGGGCT GCTCCAATGC CCACCTCCAC GGCGGCGCCA GCGTCCCCCC GGGGGGCCTG 
GGCGGCGGCG GCGGCGGGGG TAGCAGCAGC GGTAGCAGTG GCGGCGGTGG CGGTAGCGGT 
AGCGGCAGCG GCGGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAAGAAATC CAAAGAGCAA 
AAGGCGCTGC GGCTTAACAT CAATGCCCGA GAGCGCCGGC GGATGCACGA CCTGAACGAC 
GCGCTGGACG AGCTGCGCGC GGTGATCCCC TACGCGCACA GCCCCTCGGT GCGAAAGCTC 
TCCAAGATCG CCACGCTGCT GCTCGCCAAG AACTACATCC TCATGCAGGC GCAGGCCCTG 
GAGGAGATGC GGCGCCTAGT CGCCTACCTC AACCAGGGCC AGGCCATCTC GGCTGCCTCC 
CTGCCCAGCT CGGCGGCTGC AGCGGCAGCA GCTGCTGCCC TGCACCCGGC GCTCGGCGCC 
TACGAGCAGG CAGCCGGCTA CCCGTTCAGC GCCGGACTGC CCCCGGCTGC CTCCTGCCCG 
GAGAAGTGCG CCCTGTTTAA CAGCGTCTCC TCCAGCCTCT GCAAACAGTG CACGGAGAAG 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689627.1
CDS	554..1699
Translation	MERGMHLGAAAAGEDDLFLHKSLSASTSKRLEAAFRSTPPGMDLSLAPPPRERPASSSSSPLGCFEPADPEGAGLLLPPPGGGGGGSAGSGGGGGGGVGVPGLLVGSAGVGGDPSLSSLPAGAALCLKYGESASRGSVAESSGGEQSPDDDSDGRCELVLRAGVADPRASPGAGGGGAKAAEGCSNAHLHGGASVPPGGLGGGGGGGSSSGSSGGGGGSGSGSGGSSSSSSSSSKKSKEQKALRLNINARERRRMHDLNDALDELRAVIPYAHSPSVRKLSKIATLLLAKNYILMQAQALEEMRRLVAYLNQGQAISAASLPSSAAAAAAAAALHPALGAYEQAAGYPFSAGLPPAASCPEKCALFNSVSSSLCKQCTEKP

Target ORF information:

RefSeq Version	NM_152414.4
Organism	Homo sapiens(human)
Definition	Homo sapiens basic helix-loop-helix family member e22 (BHLHE22), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152414.4

ORF Insert Sequence:

ATGGAGCGCG GGATGCACCT CGGTGCAGCG GCCGCCGGCG AGGACGACCT CTTCCTGCAC 
AAGAGCCTGA GCGCCTCCAC CTCCAAGCGC TTGGAAGCGG CTTTCCGCTC CACGCCCCCG 
GGCATGGACC TGTCCCTGGC GCCGCCGCCT CGGGAACGCC CGGCGTCCTC CTCCTCGTCG 
CCCCTGGGCT GCTTCGAGCC GGCTGACCCC GAGGGGGCAG GGCTGCTGTT GCCGCCGCCT 
GGAGGAGGCG GCGGCGGCAG CGCGGGAAGT GGCGGCGGCG GCGGCGGCGG GGTGGGTGTC 
CCCGGGCTGC TAGTAGGTTC AGCCGGCGTT GGGGGCGACC CTAGCCTAAG CAGCCTGCCG 
GCCGGGGCCG CCCTTTGCCT CAAGTACGGC GAAAGCGCGA GCCGGGGCTC GGTGGCCGAG 
AGCAGCGGCG GCGAGCAGAG CCCCGACGAC GACAGCGACG GTCGCTGCGA GCTCGTGCTG 
CGGGCCGGAG TAGCCGACCC GCGGGCCTCC CCGGGAGCGG GAGGTGGTGG CGCGAAGGCA 
GCCGAGGGCT GCTCCAATGC CCACCTCCAC GGCGGCGCCA GCGTCCCCCC GGGGGGCCTG 
GGCGGCGGCG GCGGCGGGGG TAGCAGCAGC GGTAGCAGTG GCGGCGGTGG CGGTAGCGGT 
AGCGGCAGCG GCGGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAAGAAATC CAAAGAGCAA 
AAGGCGCTGC GGCTTAACAT CAATGCCCGA GAGCGCCGGC GGATGCACGA CCTGAACGAC 
GCGCTGGACG AGCTGCGCGC GGTGATCCCC TACGCGCACA GCCCCTCGGT GCGAAAGCTC 
TCCAAGATCG CCACGCTGCT GCTCGCCAAG AACTACATCC TCATGCAGGC GCAGGCCCTG 
GAGGAGATGC GGCGCCTAGT CGCCTACCTC AACCAGGGCC AGGCCATCTC GGCTGCCTCC 
CTGCCCAGCT CGGCGGCTGC AGCGGCAGCA GCTGCTGCCC TGCACCCGGC GCTCGGCGCC 
TACGAGCAGG CAGCCGGCTA CCCGTTCAGC GCCGGACTGC CCCCGGCTGC CTCCTGCCCG 
GAGAAGTGCG CCCTGTTTAA CAGCGTCTCC TCCAGCCTCT GCAAACAGTG CACGGAGAAG 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BHLHE22 ( NM_152414.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_689627.1 Homo sapiens basic helix-loop-helix family member e22 (BHLHE22), mRNA.

Price & Availability↑

CloneID	OHu08658
Clone ID Related Accession (Same CDS sequence)	NM_152414.4 , NM_152414.5
Accession Version	NM_152414.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577808000000
Organism	Homo sapiens(human)
Product	class E basic helix-loop-helix protein 22
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AA927689.1, AK293979.1, BK000273.1, AK091333.1 and AC090136.6. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_152414.4. ##Evidence-Data-START## Transcript is intronless :: BK000273.1, U80755.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000321870.3/ ENSP00000318799.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGCGCG GGATGCACCT CGGTGCAGCG GCCGCCGGCG AGGACGACCT CTTCCTGCAC 
AAGAGCCTGA GCGCCTCCAC CTCCAAGCGC TTGGAAGCGG CTTTCCGCTC CACGCCCCCG 
GGCATGGACC TGTCCCTGGC GCCGCCGCCT CGGGAACGCC CGGCGTCCTC CTCCTCGTCG 
CCCCTGGGCT GCTTCGAGCC GGCTGACCCC GAGGGGGCAG GGCTGCTGTT GCCGCCGCCT 
GGAGGAGGCG GCGGCGGCAG CGCGGGAAGT GGCGGCGGCG GCGGCGGCGG GGTGGGTGTC 
CCCGGGCTGC TAGTAGGTTC AGCCGGCGTT GGGGGCGACC CTAGCCTAAG CAGCCTGCCG 
GCCGGGGCCG CCCTTTGCCT CAAGTACGGC GAAAGCGCGA GCCGGGGCTC GGTGGCCGAG 
AGCAGCGGCG GCGAGCAGAG CCCCGACGAC GACAGCGACG GTCGCTGCGA GCTCGTGCTG 
CGGGCCGGAG TAGCCGACCC GCGGGCCTCC CCGGGAGCGG GAGGTGGTGG CGCGAAGGCA 
GCCGAGGGCT GCTCCAATGC CCACCTCCAC GGCGGCGCCA GCGTCCCCCC GGGGGGCCTG 
GGCGGCGGCG GCGGCGGGGG TAGCAGCAGC GGTAGCAGTG GCGGCGGTGG CGGTAGCGGT 
AGCGGCAGCG GCGGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAAGAAATC CAAAGAGCAA 
AAGGCGCTGC GGCTTAACAT CAATGCCCGA GAGCGCCGGC GGATGCACGA CCTGAACGAC 
GCGCTGGACG AGCTGCGCGC GGTGATCCCC TACGCGCACA GCCCCTCGGT GCGAAAGCTC 
TCCAAGATCG CCACGCTGCT GCTCGCCAAG AACTACATCC TCATGCAGGC GCAGGCCCTG 
GAGGAGATGC GGCGCCTAGT CGCCTACCTC AACCAGGGCC AGGCCATCTC GGCTGCCTCC 
CTGCCCAGCT CGGCGGCTGC AGCGGCAGCA GCTGCTGCCC TGCACCCGGC GCTCGGCGCC 
TACGAGCAGG CAGCCGGCTA CCCGTTCAGC GCCGGACTGC CCCCGGCTGC CTCCTGCCCG 
GAGAAGTGCG CCCTGTTTAA CAGCGTCTCC TCCAGCCTCT GCAAACAGTG CACGGAGAAG 
CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689627.1
CDS	427..1572
Translation	MERGMHLGAAAAGEDDLFLHKSLSASTSKRLEAAFRSTPPGMDLSLAPPPRERPASSSSSPLGCFEPADPEGAGLLLPPPGGGGGGSAGSGGGGGGGVGVPGLLVGSAGVGGDPSLSSLPAGAALCLKYGESASRGSVAESSGGEQSPDDDSDGRCELVLRAGVADPRASPGAGGGGAKAAEGCSNAHLHGGASVPPGGLGGGGGGGSSSGSSGGGGGSGSGSGGSSSSSSSSSKKSKEQKALRLNINARERRRMHDLNDALDELRAVIPYAHSPSVRKLSKIATLLLAKNYILMQAQALEEMRRLVAYLNQGQAISAASLPSSAAAAAAAAALHPALGAYEQAAGYPFSAGLPPAASCPEKCALFNSVSSSLCKQCTEKP

Target ORF information:

RefSeq Version	NM_152414.5
Organism	Homo sapiens(human)
Definition	Homo sapiens basic helix-loop-helix family member e22 (BHLHE22), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152414.5

ORF Insert Sequence:

ATGGAGCGCG GGATGCACCT CGGTGCAGCG GCCGCCGGCG AGGACGACCT CTTCCTGCAC 
AAGAGCCTGA GCGCCTCCAC CTCCAAGCGC TTGGAAGCGG CTTTCCGCTC CACGCCCCCG 
GGCATGGACC TGTCCCTGGC GCCGCCGCCT CGGGAACGCC CGGCGTCCTC CTCCTCGTCG 
CCCCTGGGCT GCTTCGAGCC GGCTGACCCC GAGGGGGCAG GGCTGCTGTT GCCGCCGCCT 
GGAGGAGGCG GCGGCGGCAG CGCGGGAAGT GGCGGCGGCG GCGGCGGCGG GGTGGGTGTC 
CCCGGGCTGC TAGTAGGTTC AGCCGGCGTT GGGGGCGACC CTAGCCTAAG CAGCCTGCCG 
GCCGGGGCCG CCCTTTGCCT CAAGTACGGC GAAAGCGCGA GCCGGGGCTC GGTGGCCGAG 
AGCAGCGGCG GCGAGCAGAG CCCCGACGAC GACAGCGACG GTCGCTGCGA GCTCGTGCTG 
CGGGCCGGAG TAGCCGACCC GCGGGCCTCC CCGGGAGCGG GAGGTGGTGG CGCGAAGGCA 
GCCGAGGGCT GCTCCAATGC CCACCTCCAC GGCGGCGCCA GCGTCCCCCC GGGGGGCCTG 
GGCGGCGGCG GCGGCGGGGG TAGCAGCAGC GGTAGCAGTG GCGGCGGTGG CGGTAGCGGT 
AGCGGCAGCG GCGGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAAGAAATC CAAAGAGCAA 
AAGGCGCTGC GGCTTAACAT CAATGCCCGA GAGCGCCGGC GGATGCACGA CCTGAACGAC 
GCGCTGGACG AGCTGCGCGC GGTGATCCCC TACGCGCACA GCCCCTCGGT GCGAAAGCTC 
TCCAAGATCG CCACGCTGCT GCTCGCCAAG AACTACATCC TCATGCAGGC GCAGGCCCTG 
GAGGAGATGC GGCGCCTAGT CGCCTACCTC AACCAGGGCC AGGCCATCTC GGCTGCCTCC 
CTGCCCAGCT CGGCGGCTGC AGCGGCAGCA GCTGCTGCCC TGCACCCGGC GCTCGGCGCC 
TACGAGCAGG CAGCCGGCTA CCCGTTCAGC GCCGGACTGC CCCCGGCTGC CTCCTGCCCG 
GAGAAGTGCG CCCTGTTTAA CAGCGTCTCC TCCAGCCTCT GCAAACAGTG CACGGAGAAG 
CCTTAA