GJA3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GJA3
Entrez Gene ID	2700
Full Name	gap junction protein alpha 3
Synonyms	CTRCT14,CX46,CZP3
General protein information	Preferred Names gap junction protein alpha 3 Names gap junction alpha-3 protein connexin-46 gap junction alpha 3 gap junction protein, alpha 3, 46kDa
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 13 Map Location: 13q12.11
Summary	The protein encoded by this gene is a connexin and is a component of lens fiber gap junctions. Defects in this gene are a cause of zonular pulverulent cataract type 3 (CZP3). [provided by RefSeq, Jan 2010].
Disorder MIM:	121015
Disorder Html:	Cataract 14, multiple types, 601885 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011535048.2	XP_011533350.1	gap junction alpha-3 protein isoform X1
NM_021954.4	NP_068773.2	gap junction alpha-3 protein
NM_021954.3	NP_068773.2	gap junction alpha-3 protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	GJA3	NP_068773.2
Gallus gallus (chicken)	GJA3	NP_001035734.1
Canis lupus familiaris (dog)	GJA3	XP_543178.2
Rattus norvegicus (Norway rat)	Gja3	NP_077352.1
Danio rerio (zebrafish)	gja3	NP_997525.1
Bos taurus (cattle)	GJA3	NP_001159768.1
Xenopus tropicalis (tropical clawed frog)	gja3	NP_001107721.1
Pan troglodytes (chimpanzee)	GJA3	XP_522616.2
Macaca mulatta (Rhesus monkey)	GJA3	XP_001087205.1
Mus musculus (house mouse)	Gja3	NP_001258552.1

Gene Ontology
Bibliography

Related articles in PubMed

Analysis of the dominant mutation N188T of human connexin46 (hCx46) using concatenation and molecular dynamics simulation.
Schadzek P, Stahl Y, Preller M, Ngezahayo A
FEBS open bio9(5)840-850(2019 05)

Structure of native lens connexin 46/50 intercellular channels by cryo-EM.
Myers JB, Haddad BG, O'Neill SE, Chorev DS, Yoshioka CC, Robinson CV, Zuckerman DM, Reichow SL
Nature564(7736)372-377(2018 12)

Alterations at Arg
Abrams CK, Peinado A, Mahmoud R, Bocarsly M, Zhang H, Chang P, Botello-Smith WM, Freidin MM, Luo Y
American journal of physiology. Cell physiology315(5)C623-C635(2018 11)

Whole-genome sequencing reveals a recurrent missense mutation in the Connexin 46 (GJA3) gene causing autosomal-dominant lamellar cataract.
Berry V, Ionides ACW, Pontikos N, Moghul I, Moore AT, Cheetham ME, Michaelides M
Eye (London, England)321661-1668(2018 05)

Cataract-associated connexin 46 mutation alters its interaction with calmodulin and function of hemichannels.
Hu Z, Riquelme MA, Wang B, Bugay V, Brenner R, Gu S, Jiang JX
The Journal of biological chemistry293(7)2573-2585(2018 02)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GJA3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GJA3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25530	NM_021954.4 Latest version!	Homo sapiens gap junction protein alpha 3 (GJA3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$258.30 $369.00
OHu25530	XM_011535048.2 Latest version!	Homo sapiens gap junction protein alpha 3 (GJA3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$258.30 $369.00
View Old Accession Versions >>
OHu25530	NM_021954.3	Homo sapiens gap junction protein alpha 3 (GJA3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GJA3 ( NM_021954.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_068773.2 Homo sapiens gap junction protein alpha 3 (GJA3), mRNA.

Price & Availability↑

CloneID	OHu25530
Clone ID Related Accession (Same CDS sequence)	XM_011535048.2 , NM_021954.4 , NM_021954.3
Accession Version	NM_021954.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1574006400000
Organism	Homo sapiens(human)
Product	gap junction alpha-3 protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL138688.27, DR003755.1 and BF726530.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_021954.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DR003755.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000241125.4/ ENSP00000241125.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCGACT GGAGCTTTCT GGGAAGACTC TTAGAAAATG CACAGGAGCA CTCCACGGTC 
ATCGGCAAGG TTTGGCTGAC CGTGCTGTTC ATCTTCCGCA TCTTGGTGCT GGGGGCCGCG 
GCGGAGGACG TGTGGGGCGA TGAGCAGTCA GACTTCACCT GCAACACCCA GCAGCCGGGC 
TGCGAGAACG TCTGCTACGA CAGGGCCTTC CCCATCTCCC ACATCCGCTT CTGGGCGCTG 
CAGATCATCT TCGTGTCCAC GCCCACCCTC ATCTACCTGG GCCACGTGCT GCACATCGTG 
CGCATGGAAG AGAAGAAGAA AGAGAGGGAG GAGGAGGAGC AGCTGAAGAG AGAGAGCCCC 
AGCCCCAAGG AGCCACCGCA GGACAATCCC TCGTCGCGGG ACGACCGCGG CAGGGTGCGC 
ATGGCCGGGG CGCTGCTGCG GACCTACGTC TTCAACATCA TCTTCAAGAC GCTGTTCGAG 
GTGGGCTTCA TCGCCGGCCA GTACTTTCTG TACGGCTTCG AGCTGAAGCC GCTCTACCGC 
TGCGACCGCT GGCCCTGCCC CAACACGGTG GACTGCTTCA TCTCCAGGCC CACGGAGAAG 
ACCATCTTCA TCATCTTCAT GCTGGCGGTG GCCTGCGCGT CCCTGCTGCT CAACATGCTG 
GAGATCTACC ACCTGGGCTG GAAGAAGCTC AAGCAGGGCG TGACCAGCCG CCTCGGCCCG 
GACGCCTCCG AGGCCCCGCT GGGGACAGCC GATCCCCCGC CCCTGCCCCC CAGCTCCCGG 
CCGCCCGCCG TTGCCATCGG GTTCCCACCC TACTATGCGC ACACCGCTGC GCCCCTGGGA 
CAGGCCCGCG CCGTGGGCTA CCCCGGGGCC CCGCCACCAG CCGCGGACTT CAAACTGCTA 
GCCCTGACCG AGGCGCGCGG AAAGGGCCAG TCCGCCAAGC TCTACAACGG CCACCACCAC 
CTGCTGATGA CTGAGCAGAA CTGGGCCAAC CAGGCGGCCG AGCGGCAGCC CCCGGCGCTC 
AAGGCTTACC CGGCAGCGTC CACGCCTGCA GCCCCCAGCC CCGTCGGCAG CAGCTCCCCG 
CCACTCGCGC ACGAGGCTGA GGCGGGCGCG GCGCCCCTGC TGCTGGATGG GAGCGGCAGC 
AGTCTGGAGG GGAGCGCCCT GGCAGGGACC CCCGAGGAGG AGGAGCAGGC CGTGACCACC 
GCGGCCCAGA TGCACCAGCC GCCCTTGCCC CTCGGAGACC CAGGTCGGGC CAGCAAGGCC 
AGCAGGGCCA GCAGCGGGCG GGCCAGACCG GAGGACTTGG CCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068773.2
CDS	181..1488
Translation	MGDWSFLGRLLENAQEHSTVIGKVWLTVLFIFRILVLGAAAEDVWGDEQSDFTCNTQQPGCENVCYDRAFPISHIRFWALQIIFVSTPTLIYLGHVLHIVRMEEKKKEREEEEQLKRESPSPKEPPQDNPSSRDDRGRVRMAGALLRTYVFNIIFKTLFEVGFIAGQYFLYGFELKPLYRCDRWPCPNTVDCFISRPTEKTIFIIFMLAVACASLLLNMLEIYHLGWKKLKQGVTSRLGPDASEAPLGTADPPPLPPSSRPPAVAIGFPPYYAHTAAPLGQARAVGYPGAPPPAADFKLLALTEARGKGQSAKLYNGHHHLLMTEQNWANQAAERQPPALKAYPAASTPAAPSPVGSSSPPLAHEAEAGAAPLLLDGSGSSLEGSALAGTPEEEEQAVTTAAQMHQPPLPLGDPGRASKASRASSGRARPEDLAI

Target ORF information:

RefSeq Version	NM_021954.4
Organism	Homo sapiens(human)
Definition	Homo sapiens gap junction protein alpha 3 (GJA3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021954.4

ORF Insert Sequence:

ATGGGCGACT GGAGCTTTCT GGGAAGACTC TTAGAAAATG CACAGGAGCA CTCCACGGTC 
ATCGGCAAGG TTTGGCTGAC CGTGCTGTTC ATCTTCCGCA TCTTGGTGCT GGGGGCCGCG 
GCGGAGGACG TGTGGGGCGA TGAGCAGTCA GACTTCACCT GCAACACCCA GCAGCCGGGC 
TGCGAGAACG TCTGCTACGA CAGGGCCTTC CCCATCTCCC ACATCCGCTT CTGGGCGCTG 
CAGATCATCT TCGTGTCCAC GCCCACCCTC ATCTACCTGG GCCACGTGCT GCACATCGTG 
CGCATGGAAG AGAAGAAGAA AGAGAGGGAG GAGGAGGAGC AGCTGAAGAG AGAGAGCCCC 
AGCCCCAAGG AGCCACCGCA GGACAATCCC TCGTCGCGGG ACGACCGCGG CAGGGTGCGC 
ATGGCCGGGG CGCTGCTGCG GACCTACGTC TTCAACATCA TCTTCAAGAC GCTGTTCGAG 
GTGGGCTTCA TCGCCGGCCA GTACTTTCTG TACGGCTTCG AGCTGAAGCC GCTCTACCGC 
TGCGACCGCT GGCCCTGCCC CAACACGGTG GACTGCTTCA TCTCCAGGCC CACGGAGAAG 
ACCATCTTCA TCATCTTCAT GCTGGCGGTG GCCTGCGCGT CCCTGCTGCT CAACATGCTG 
GAGATCTACC ACCTGGGCTG GAAGAAGCTC AAGCAGGGCG TGACCAGCCG CCTCGGCCCG 
GACGCCTCCG AGGCCCCGCT GGGGACAGCC GATCCCCCGC CCCTGCCCCC CAGCTCCCGG 
CCGCCCGCCG TTGCCATCGG GTTCCCACCC TACTATGCGC ACACCGCTGC GCCCCTGGGA 
CAGGCCCGCG CCGTGGGCTA CCCCGGGGCC CCGCCACCAG CCGCGGACTT CAAACTGCTA 
GCCCTGACCG AGGCGCGCGG AAAGGGCCAG TCCGCCAAGC TCTACAACGG CCACCACCAC 
CTGCTGATGA CTGAGCAGAA CTGGGCCAAC CAGGCGGCCG AGCGGCAGCC CCCGGCGCTC 
AAGGCTTACC CGGCAGCGTC CACGCCTGCA GCCCCCAGCC CCGTCGGCAG CAGCTCCCCG 
CCACTCGCGC ACGAGGCTGA GGCGGGCGCG GCGCCCCTGC TGCTGGATGG GAGCGGCAGC 
AGTCTGGAGG GGAGCGCCCT GGCAGGGACC CCCGAGGAGG AGGAGCAGGC CGTGACCACC 
GCGGCCCAGA TGCACCAGCC GCCCTTGCCC CTCGGAGACC CAGGTCGGGC CAGCAAGGCC 
AGCAGGGCCA GCAGCGGGCG GGCCAGACCG GAGGACTTGG CCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GJA3 ( NM_021954.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_068773.2 Homo sapiens gap junction protein alpha 3 (GJA3), mRNA.

Price & Availability↑

CloneID	OHu25530
Clone ID Related Accession (Same CDS sequence)	XM_011535048.2 , NM_021954.4 , NM_021954.3
Accession Version	NM_021954.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1509379200000
Organism	Homo sapiens(human)
Product	gap junction alpha-3 protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR003755.1, AL138688.27 and BF726530.1. This sequence is a reference standard in the RefSeqGene project. On Sep 29, 2006 this sequence version replaced NM_021954.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DR003755.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGACT GGAGCTTTCT GGGAAGACTC TTAGAAAATG CACAGGAGCA CTCCACGGTC 
ATCGGCAAGG TTTGGCTGAC CGTGCTGTTC ATCTTCCGCA TCTTGGTGCT GGGGGCCGCG 
GCGGAGGACG TGTGGGGCGA TGAGCAGTCA GACTTCACCT GCAACACCCA GCAGCCGGGC 
TGCGAGAACG TCTGCTACGA CAGGGCCTTC CCCATCTCCC ACATCCGCTT CTGGGCGCTG 
CAGATCATCT TCGTGTCCAC GCCCACCCTC ATCTACCTGG GCCACGTGCT GCACATCGTG 
CGCATGGAAG AGAAGAAGAA AGAGAGGGAG GAGGAGGAGC AGCTGAAGAG AGAGAGCCCC 
AGCCCCAAGG AGCCACCGCA GGACAATCCC TCGTCGCGGG ACGACCGCGG CAGGGTGCGC 
ATGGCCGGGG CGCTGCTGCG GACCTACGTC TTCAACATCA TCTTCAAGAC GCTGTTCGAG 
GTGGGCTTCA TCGCCGGCCA GTACTTTCTG TACGGCTTCG AGCTGAAGCC GCTCTACCGC 
TGCGACCGCT GGCCCTGCCC CAACACGGTG GACTGCTTCA TCTCCAGGCC CACGGAGAAG 
ACCATCTTCA TCATCTTCAT GCTGGCGGTG GCCTGCGCGT CCCTGCTGCT CAACATGCTG 
GAGATCTACC ACCTGGGCTG GAAGAAGCTC AAGCAGGGCG TGACCAGCCG CCTCGGCCCG 
GACGCCTCCG AGGCCCCGCT GGGGACAGCC GATCCCCCGC CCCTGCCCCC CAGCTCCCGG 
CCGCCCGCCG TTGCCATCGG GTTCCCACCC TACTATGCGC ACACCGCTGC GCCCCTGGGA 
CAGGCCCGCG CCGTGGGCTA CCCCGGGGCC CCGCCACCAG CCGCGGACTT CAAACTGCTA 
GCCCTGACCG AGGCGCGCGG AAAGGGCCAG TCCGCCAAGC TCTACAACGG CCACCACCAC 
CTGCTGATGA CTGAGCAGAA CTGGGCCAAC CAGGCGGCCG AGCGGCAGCC CCCGGCGCTC 
AAGGCTTACC CGGCAGCGTC CACGCCTGCA GCCCCCAGCC CCGTCGGCAG CAGCTCCCCG 
CCACTCGCGC ACGAGGCTGA GGCGGGCGCG GCGCCCCTGC TGCTGGATGG GAGCGGCAGC 
AGTCTGGAGG GGAGCGCCCT GGCAGGGACC CCCGAGGAGG AGGAGCAGGC CGTGACCACC 
GCGGCCCAGA TGCACCAGCC GCCCTTGCCC CTCGGAGACC CAGGTCGGGC CAGCAAGGCC 
AGCAGGGCCA GCAGCGGGCG GGCCAGACCG GAGGACTTGG CCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068773.2
CDS	173..1480
Translation	MGDWSFLGRLLENAQEHSTVIGKVWLTVLFIFRILVLGAAAEDVWGDEQSDFTCNTQQPGCENVCYDRAFPISHIRFWALQIIFVSTPTLIYLGHVLHIVRMEEKKKEREEEEQLKRESPSPKEPPQDNPSSRDDRGRVRMAGALLRTYVFNIIFKTLFEVGFIAGQYFLYGFELKPLYRCDRWPCPNTVDCFISRPTEKTIFIIFMLAVACASLLLNMLEIYHLGWKKLKQGVTSRLGPDASEAPLGTADPPPLPPSSRPPAVAIGFPPYYAHTAAPLGQARAVGYPGAPPPAADFKLLALTEARGKGQSAKLYNGHHHLLMTEQNWANQAAERQPPALKAYPAASTPAAPSPVGSSSPPLAHEAEAGAAPLLLDGSGSSLEGSALAGTPEEEEQAVTTAAQMHQPPLPLGDPGRASKASRASSGRARPEDLAI

Target ORF information:

RefSeq Version	NM_021954.3
Organism	Homo sapiens(human)
Definition	Homo sapiens gap junction protein alpha 3 (GJA3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021954.3

ORF Insert Sequence:

ATGGGCGACT GGAGCTTTCT GGGAAGACTC TTAGAAAATG CACAGGAGCA CTCCACGGTC 
ATCGGCAAGG TTTGGCTGAC CGTGCTGTTC ATCTTCCGCA TCTTGGTGCT GGGGGCCGCG 
GCGGAGGACG TGTGGGGCGA TGAGCAGTCA GACTTCACCT GCAACACCCA GCAGCCGGGC 
TGCGAGAACG TCTGCTACGA CAGGGCCTTC CCCATCTCCC ACATCCGCTT CTGGGCGCTG 
CAGATCATCT TCGTGTCCAC GCCCACCCTC ATCTACCTGG GCCACGTGCT GCACATCGTG 
CGCATGGAAG AGAAGAAGAA AGAGAGGGAG GAGGAGGAGC AGCTGAAGAG AGAGAGCCCC 
AGCCCCAAGG AGCCACCGCA GGACAATCCC TCGTCGCGGG ACGACCGCGG CAGGGTGCGC 
ATGGCCGGGG CGCTGCTGCG GACCTACGTC TTCAACATCA TCTTCAAGAC GCTGTTCGAG 
GTGGGCTTCA TCGCCGGCCA GTACTTTCTG TACGGCTTCG AGCTGAAGCC GCTCTACCGC 
TGCGACCGCT GGCCCTGCCC CAACACGGTG GACTGCTTCA TCTCCAGGCC CACGGAGAAG 
ACCATCTTCA TCATCTTCAT GCTGGCGGTG GCCTGCGCGT CCCTGCTGCT CAACATGCTG 
GAGATCTACC ACCTGGGCTG GAAGAAGCTC AAGCAGGGCG TGACCAGCCG CCTCGGCCCG 
GACGCCTCCG AGGCCCCGCT GGGGACAGCC GATCCCCCGC CCCTGCCCCC CAGCTCCCGG 
CCGCCCGCCG TTGCCATCGG GTTCCCACCC TACTATGCGC ACACCGCTGC GCCCCTGGGA 
CAGGCCCGCG CCGTGGGCTA CCCCGGGGCC CCGCCACCAG CCGCGGACTT CAAACTGCTA 
GCCCTGACCG AGGCGCGCGG AAAGGGCCAG TCCGCCAAGC TCTACAACGG CCACCACCAC 
CTGCTGATGA CTGAGCAGAA CTGGGCCAAC CAGGCGGCCG AGCGGCAGCC CCCGGCGCTC 
AAGGCTTACC CGGCAGCGTC CACGCCTGCA GCCCCCAGCC CCGTCGGCAG CAGCTCCCCG 
CCACTCGCGC ACGAGGCTGA GGCGGGCGCG GCGCCCCTGC TGCTGGATGG GAGCGGCAGC 
AGTCTGGAGG GGAGCGCCCT GGCAGGGACC CCCGAGGAGG AGGAGCAGGC CGTGACCACC 
GCGGCCCAGA TGCACCAGCC GCCCTTGCCC CTCGGAGACC CAGGTCGGGC CAGCAAGGCC 
AGCAGGGCCA GCAGCGGGCG GGCCAGACCG GAGGACTTGG CCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GJA3 ( XM_011535048.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011533350.1 Homo sapiens gap junction protein alpha 3 (GJA3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu25530
Clone ID Related Accession (Same CDS sequence)	XM_011535048.2 , NM_021954.4 , NM_021954.3
Accession Version	XM_011535048.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	gap junction alpha-3 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535048.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACT GGAGCTTTCT GGGAAGACTC TTAGAAAATG CACAGGAGCA CTCCACGGTC 
ATCGGCAAGG TTTGGCTGAC CGTGCTGTTC ATCTTCCGCA TCTTGGTGCT GGGGGCCGCG 
GCGGAGGACG TGTGGGGCGA TGAGCAGTCA GACTTCACCT GCAACACCCA GCAGCCGGGC 
TGCGAGAACG TCTGCTACGA CAGGGCCTTC CCCATCTCCC ACATCCGCTT CTGGGCGCTG 
CAGATCATCT TCGTGTCCAC GCCCACCCTC ATCTACCTGG GCCACGTGCT GCACATCGTG 
CGCATGGAAG AGAAGAAGAA AGAGAGGGAG GAGGAGGAGC AGCTGAAGAG AGAGAGCCCC 
AGCCCCAAGG AGCCACCGCA GGACAATCCC TCGTCGCGGG ACGACCGCGG CAGGGTGCGC 
ATGGCCGGGG CGCTGCTGCG GACCTACGTC TTCAACATCA TCTTCAAGAC GCTGTTCGAG 
GTGGGCTTCA TCGCCGGCCA GTACTTTCTG TACGGCTTCG AGCTGAAGCC GCTCTACCGC 
TGCGACCGCT GGCCCTGCCC CAACACGGTG GACTGCTTCA TCTCCAGGCC CACGGAGAAG 
ACCATCTTCA TCATCTTCAT GCTGGCGGTG GCCTGCGCGT CCCTGCTGCT CAACATGCTG 
GAGATCTACC ACCTGGGCTG GAAGAAGCTC AAGCAGGGCG TGACCAGCCG CCTCGGCCCG 
GACGCCTCCG AGGCCCCGCT GGGGACAGCC GATCCCCCGC CCCTGCCCCC CAGCTCCCGG 
CCGCCCGCCG TTGCCATCGG GTTCCCACCC TACTATGCGC ACACCGCTGC GCCCCTGGGA 
CAGGCCCGCG CCGTGGGCTA CCCCGGGGCC CCGCCACCAG CCGCGGACTT CAAACTGCTA 
GCCCTGACCG AGGCGCGCGG AAAGGGCCAG TCCGCCAAGC TCTACAACGG CCACCACCAC 
CTGCTGATGA CTGAGCAGAA CTGGGCCAAC CAGGCGGCCG AGCGGCAGCC CCCGGCGCTC 
AAGGCTTACC CGGCAGCGTC CACGCCTGCA GCCCCCAGCC CCGTCGGCAG CAGCTCCCCG 
CCACTCGCGC ACGAGGCTGA GGCGGGCGCG GCGCCCCTGC TGCTGGATGG GAGCGGCAGC 
AGTCTGGAGG GGAGCGCCCT GGCAGGGACC CCCGAGGAGG AGGAGCAGGC CGTGACCACC 
GCGGCCCAGA TGCACCAGCC GCCCTTGCCC CTCGGAGACC CAGGTCGGGC CAGCAAGGCC 
AGCAGGGCCA GCAGCGGGCG GGCCAGACCG GAGGACTTGG CCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011533350.1
CDS	80..1387
Translation	MGDWSFLGRLLENAQEHSTVIGKVWLTVLFIFRILVLGAAAEDVWGDEQSDFTCNTQQPGCENVCYDRAFPISHIRFWALQIIFVSTPTLIYLGHVLHIVRMEEKKKEREEEEQLKRESPSPKEPPQDNPSSRDDRGRVRMAGALLRTYVFNIIFKTLFEVGFIAGQYFLYGFELKPLYRCDRWPCPNTVDCFISRPTEKTIFIIFMLAVACASLLLNMLEIYHLGWKKLKQGVTSRLGPDASEAPLGTADPPPLPPSSRPPAVAIGFPPYYAHTAAPLGQARAVGYPGAPPPAADFKLLALTEARGKGQSAKLYNGHHHLLMTEQNWANQAAERQPPALKAYPAASTPAAPSPVGSSSPPLAHEAEAGAAPLLLDGSGSSLEGSALAGTPEEEEQAVTTAAQMHQPPLPLGDPGRASKASRASSGRARPEDLAI

Target ORF information:

RefSeq Version	XM_011535048.2
Organism	Homo sapiens(human)
Definition	Homo sapiens gap junction protein alpha 3 (GJA3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535048.2

ORF Insert Sequence:

ATGGGCGACT GGAGCTTTCT GGGAAGACTC TTAGAAAATG CACAGGAGCA CTCCACGGTC 
ATCGGCAAGG TTTGGCTGAC CGTGCTGTTC ATCTTCCGCA TCTTGGTGCT GGGGGCCGCG 
GCGGAGGACG TGTGGGGCGA TGAGCAGTCA GACTTCACCT GCAACACCCA GCAGCCGGGC 
TGCGAGAACG TCTGCTACGA CAGGGCCTTC CCCATCTCCC ACATCCGCTT CTGGGCGCTG 
CAGATCATCT TCGTGTCCAC GCCCACCCTC ATCTACCTGG GCCACGTGCT GCACATCGTG 
CGCATGGAAG AGAAGAAGAA AGAGAGGGAG GAGGAGGAGC AGCTGAAGAG AGAGAGCCCC 
AGCCCCAAGG AGCCACCGCA GGACAATCCC TCGTCGCGGG ACGACCGCGG CAGGGTGCGC 
ATGGCCGGGG CGCTGCTGCG GACCTACGTC TTCAACATCA TCTTCAAGAC GCTGTTCGAG 
GTGGGCTTCA TCGCCGGCCA GTACTTTCTG TACGGCTTCG AGCTGAAGCC GCTCTACCGC 
TGCGACCGCT GGCCCTGCCC CAACACGGTG GACTGCTTCA TCTCCAGGCC CACGGAGAAG 
ACCATCTTCA TCATCTTCAT GCTGGCGGTG GCCTGCGCGT CCCTGCTGCT CAACATGCTG 
GAGATCTACC ACCTGGGCTG GAAGAAGCTC AAGCAGGGCG TGACCAGCCG CCTCGGCCCG 
GACGCCTCCG AGGCCCCGCT GGGGACAGCC GATCCCCCGC CCCTGCCCCC CAGCTCCCGG 
CCGCCCGCCG TTGCCATCGG GTTCCCACCC TACTATGCGC ACACCGCTGC GCCCCTGGGA 
CAGGCCCGCG CCGTGGGCTA CCCCGGGGCC CCGCCACCAG CCGCGGACTT CAAACTGCTA 
GCCCTGACCG AGGCGCGCGG AAAGGGCCAG TCCGCCAAGC TCTACAACGG CCACCACCAC 
CTGCTGATGA CTGAGCAGAA CTGGGCCAAC CAGGCGGCCG AGCGGCAGCC CCCGGCGCTC 
AAGGCTTACC CGGCAGCGTC CACGCCTGCA GCCCCCAGCC CCGTCGGCAG CAGCTCCCCG 
CCACTCGCGC ACGAGGCTGA GGCGGGCGCG GCGCCCCTGC TGCTGGATGG GAGCGGCAGC 
AGTCTGGAGG GGAGCGCCCT GGCAGGGACC CCCGAGGAGG AGGAGCAGGC CGTGACCACC 
GCGGCCCAGA TGCACCAGCC GCCCTTGCCC CTCGGAGACC CAGGTCGGGC CAGCAAGGCC 
AGCAGGGCCA GCAGCGGGCG GGCCAGACCG GAGGACTTGG CCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Analysis of the dominant mutation N188T of human connexin46 (hCx46) using concatenation and molecular dynamics simulation.
FEBS open bio9(5)840-850(2019 05)
Schadzek P,Stahl Y,Preller M,Ngezahayo A

Structure of native lens connexin 46/50 intercellular channels by cryo-EM.
Nature564(7736)372-377(2018 12)
Myers JB,Haddad BG,O'Neill SE,Chorev DS,Yoshioka CC,Robinson CV,Zuckerman DM,Reichow SL

Alterations at Arg
American journal of physiology. Cell physiology315(5)C623-C635(2018 11)
Abrams CK,Peinado A,Mahmoud R,Bocarsly M,Zhang H,Chang P,Botello-Smith WM,Freidin MM,Luo Y

Whole-genome sequencing reveals a recurrent missense mutation in the Connexin 46 (GJA3) gene causing autosomal-dominant lamellar cataract.
Eye (London, England)321661-1668(2018 05)
Berry V,Ionides ACW,Pontikos N,Moghul I,Moore AT,Cheetham ME,Michaelides M

Cataract-associated connexin 46 mutation alters its interaction with calmodulin and function of hemichannels.
The Journal of biological chemistry293(7)2573-2585(2018 02)
Hu Z,Riquelme MA,Wang B,Bugay V,Brenner R,Gu S,Jiang JX

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GJA3 cDNA ORF clone, Homo sapiens(human)

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