HIBCH cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HIBCH
Entrez Gene ID	26275
Full Name	3-hydroxyisobutyryl-CoA hydrolase
Synonyms	HIBYLCOAH
General protein information	Preferred Names 3-hydroxyisobutyryl-CoA hydrolase Names 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial 3-hydroxyisobutyryl-Coenzyme A hydrolase HIB-CoA hydrolase HIBYL-CoA-H testicular tissue protein Li 86
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q32.2
Summary	This gene encodes the enzyme responsible for hydrolysis of both HIBYL-CoA and beta-hydroxypropionyl-CoA. Mutations in this gene have been associated with 3-hyroxyisobutyryl-CoA hydrolase deficiency. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010].
Disorder MIM:	610690
Disorder Html:	3-hydroxyisobutryl-CoA hydrolase deficiency, 250620 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011510953.2	XP_011509255.1	3-hydroxyisobutyryl-CoA hydrolase, mitochondrial isoform X1
NM_014362.4	NP_055177.2	3-hydroxyisobutyryl-CoA hydrolase, mitochondrial isoform 1 precursor
NM_198047.3	NP_932164.1	3-hydroxyisobutyryl-CoA hydrolase, mitochondrial isoform 2 precursor
NM_198047.2	NP_932164.1	3-hydroxyisobutyryl-CoA hydrolase, mitochondrial isoform 2 precursor
NM_014362.3	NP_055177.2	3-hydroxyisobutyryl-CoA hydrolase, mitochondrial isoform 1 precursor
XM_011510953.1	XP_011509255.1	3-hydroxyisobutyryl-CoA hydrolase, mitochondrial isoform X1
XM_011510954.1	XP_011509256.1	3-hydroxyisobutyryl-CoA hydrolase, mitochondrial isoform X2

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0F18678g	XP_455917.1
Arabidopsis thaliana (thale cress)	CHY1	NP_201395.1
Schizosaccharomyces pombe (fission yeast)	SPBC2D10.09	NP_596228.1
Magnaporthe oryzae (rice blast fungus)	MGG_04012	XP_003719838.1
Neurospora crassa	NCU03777	XP_961123.1
Homo sapiens (human)	HIBCH	NP_055177.2
Pan troglodytes (chimpanzee)	HIBCH	XP_515988.3
Rattus norvegicus (Norway rat)	Hibch	NP_001013130.1
Saccharomyces cerevisiae (baker's yeast)	EHD3	NP_010321.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004097	XP_312972.5
Macaca mulatta (Rhesus monkey)	LOC713951	XP_001103467.1
Mus musculus (house mouse)	Hibch	NP_666220.1
Drosophila melanogaster (fruit fly)	CG5044	NP_650453.3
Caenorhabditis elegans (roundworm)	F09F7.4	NP_741143.1
Arabidopsis thaliana (thale cress)	AT2G30650	NP_180623.3
Xenopus tropicalis (tropical clawed frog)	hibch	NP_001016188.1
Oryza sativa (rice)	Os12g0264500	NP_001066529.1
Canis lupus familiaris (dog)	HIBCH	XP_005640511.1
Danio rerio (zebrafish)	hibch	NP_001014338.1
Bos taurus (cattle)	HIBCH	NP_001039878.1
Gallus gallus (chicken)	HIBCH	NP_001026414.1
Arabidopsis thaliana (thale cress)	AT2G30660	NP_180624.2
Eremothecium gossypii	AGOS_AGR024C	NP_986689.1

Gene Ontology
Bibliography

Related articles in PubMed

A movement disorder with dystonia and ataxia caused by a mutation in the HIBCH gene.
Schottmann G, Sarpong A, Lorenz C, Weinhold N, Gill E, Teschner L, Ferdinandusse S, Wanders RJ, Prigione A, Schuelke M
Movement disorders : official journal of the Movement Disorder Society31(11)1733-1739(2016 11)

A Common Polymorphism in HIBCH Influences Methylmalonic Acid Concentrations in Blood Independently of Cobalamin.
Molloy AM, Pangilinan F, Mills JL, Shane B, O'Neill MB, McGaughey DM, Velkova A, Abaan HO, Ueland PM, McNulty H, Ward M, Strain JJ, Cunningham C, Casey M, Cropp CD, Kim Y, Bailey-Wilson JE, Wilson AF, Brody LC
American journal of human genetics98(5)869-882(2016 May)

HIBCH mutations can cause Leigh-like disease with combined deficiency of multiple mitochondrial respiratory chain enzymes and pyruvate dehydrogenase.
Ferdinandusse S, Waterham HR, Heales SJ, Brown GK, Hargreaves IP, Taanman JW, Gunny R, Abulhoul L, Wanders RJ, Clayton PT, Leonard JV, Rahman S
Orphanet journal of rare diseases8188(2013 Dec)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screening.
Wu C, Ma MH, Brown KR, Geisler M, Li L, Tzeng E, Jia CY, Jurisica I, Li SS
Proteomics7(11)1775-85(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HIBCH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIBCH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15155	NM_198047.3 Latest version!	Homo sapiens 3-hydroxyisobutyryl-CoA hydrolase (HIBCH), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu66919	XM_011510954.1 Latest version!	Homo sapiens 3-hydroxyisobutyryl-CoA hydrolase (HIBCH), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu23117	NM_014362.4 Latest version!	Homo sapiens 3-hydroxyisobutyryl-CoA hydrolase (HIBCH), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$258.30 $369.00
OHu23117	XM_011510953.2 Latest version!	Homo sapiens 3-hydroxyisobutyryl-CoA hydrolase (HIBCH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$258.30 $369.00
View Old Accession Versions >>
OHu15155	NM_198047.2	Homo sapiens 3-hydroxyisobutyryl-CoA hydrolase (HIBCH), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu23117	NM_014362.3	Homo sapiens 3-hydroxyisobutyryl-CoA hydrolase (HIBCH), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$258.30 $369.00
OHu23117	XM_011510953.1	Homo sapiens 3-hydroxyisobutyryl-CoA hydrolase (HIBCH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HIBCH ( NM_198047.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_932164.1 Homo sapiens 3-hydroxyisobutyryl-CoA hydrolase (HIBCH), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu15155
Clone ID Related Accession (Same CDS sequence)	NM_198047.3 , NM_198047.2
Accession Version	NM_198047.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577980800000
Organism	Homo sapiens(human)
Product	3-hydroxyisobutyryl-CoA hydrolase, mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092178.4, BC005190.1, AK223023.1 and AC010679.6. On May 31, 2019 this sequence version replaced NM_198047.2. Transcript Variant: This variant (2) lacks an exon in the 3' coding region, which results in a frameshift and an early stop codon, compared to variant 1. The encoded isoform (2) has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.102143.1, SRR1660803.151776.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGCAGC GCGAGATGTG GAGGCTCATG TCGAGGTTTA ATGCATTCAA AAGGACTAAT 
ACCATACTGC ACCATTTGAG AATGTCCAAG CACACAGATG CAGCAGAAGA GGTGCTATTG 
GAAAAAAAAG GTTGCACGGG AGTCATAACA CTAAACAGAC CAAAGTTCCT CAATGCACTG 
ACTCTTAATA TGATTCGGCA GATTTATCCA CAGCTAAAGA AGTGGGAACA AGATCCTGAA 
ACTTTCCTGA TCATTATAAA GGGAGCAGGA GGAAAGGCTT TCTGTGCCGG GGGTGATATC 
AGAGTGATCT CGGAAGCTGA AAAGGCAAAA CAGAAGATAG CTCCAGTTTT CTTCAGAGAA 
GAATATATGC TGAATAATGC TGTTGGTTCT TGCCAGAAAC CTTATGTTGC ACTTATTCAT 
GGAATTACAA TGGGTGGGGG AGTTGGTCTC TCAGTCCATG GGCAATTTCG AGTGGCTACA 
GAAAAGTGTC TTTTTGCTAT GCCAGAAACT GCAATAGGAC TGTTCCCTGA TGTGGGTGGA 
GGTTATTTCT TGCCACGACT CCAAGGAAAA CTTGGTTACT TCCTTGCATT AACAGGATTC 
AGACTAAAAG GAAGAGATGT GTACAGAGCA GGAATTGCTA CACACTTTGT AGATTCTGAA 
AAGTTGGCCA TGTTAGAGGA AGATTTGTTA GCCTTGAAAT CTCCTTCAAA AGAAAATATT 
GCATCTGTCT TAGAAAATTA CCATACAGAG TCTAAGATTG ATCGAGACAA GTCTTTTATA 
CTTGAGGAAC ACATGGACAA AATAAACAGT TGTTTTTCAG CCAATACTGT GGAAGAAATT 
ATTGAAAACT TACAGCAAGA TGGTTCATCT TTTGCCCTAG AGCAATTGAA GGTAATTAAT 
AAAATGTCTC CAACATCTCT AAAGATCACA CTAAGGCAAC TCATGGAGGG GTCTTCAAAG 
ACCTTGCAAG AAGTACTAAC TATGGAGTAT CGGCTAAGTC AAGCTTGTAT GTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_932164.1
CDS	77..1093
Translation	MGQREMWRLMSRFNAFKRTNTILHHLRMSKHTDAAEEVLLEKKGCTGVITLNRPKFLNALTLNMIRQIYPQLKKWEQDPETFLIIIKGAGGKAFCAGGDIRVISEAEKAKQKIAPVFFREEYMLNNAVGSCQKPYVALIHGITMGGGVGLSVHGQFRVATEKCLFAMPETAIGLFPDVGGGYFLPRLQGKLGYFLALTGFRLKGRDVYRAGIATHFVDSEKLAMLEEDLLALKSPSKENIASVLENYHTESKIDRDKSFILEEHMDKINSCFSANTVEEIIENLQQDGSSFALEQLKVINKMSPTSLKITLRQLMEGSSKTLQEVLTMEYRLSQACMF

Target ORF information:

RefSeq Version	NM_198047.3
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxyisobutyryl-CoA hydrolase (HIBCH), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198047.3

ORF Insert Sequence:

ATGGGGCAGC GCGAGATGTG GAGGCTCATG TCGAGGTTTA ATGCATTCAA AAGGACTAAT 
ACCATACTGC ACCATTTGAG AATGTCCAAG CACACAGATG CAGCAGAAGA GGTGCTATTG 
GAAAAAAAAG GTTGCACGGG AGTCATAACA CTAAACAGAC CAAAGTTCCT CAATGCACTG 
ACTCTTAATA TGATTCGGCA GATTTATCCA CAGCTAAAGA AGTGGGAACA AGATCCTGAA 
ACTTTCCTGA TCATTATAAA GGGAGCAGGA GGAAAGGCTT TCTGTGCCGG GGGTGATATC 
AGAGTGATCT CGGAAGCTGA AAAGGCAAAA CAGAAGATAG CTCCAGTTTT CTTCAGAGAA 
GAATATATGC TGAATAATGC TGTTGGTTCT TGCCAGAAAC CTTATGTTGC ACTTATTCAT 
GGAATTACAA TGGGTGGGGG AGTTGGTCTC TCAGTCCATG GGCAATTTCG AGTGGCTACA 
GAAAAGTGTC TTTTTGCTAT GCCAGAAACT GCAATAGGAC TGTTCCCTGA TGTGGGTGGA 
GGTTATTTCT TGCCACGACT CCAAGGAAAA CTTGGTTACT TCCTTGCATT AACAGGATTC 
AGACTAAAAG GAAGAGATGT GTACAGAGCA GGAATTGCTA CACACTTTGT AGATTCTGAA 
AAGTTGGCCA TGTTAGAGGA AGATTTGTTA GCCTTGAAAT CTCCTTCAAA AGAAAATATT 
GCATCTGTCT TAGAAAATTA CCATACAGAG TCTAAGATTG ATCGAGACAA GTCTTTTATA 
CTTGAGGAAC ACATGGACAA AATAAACAGT TGTTTTTCAG CCAATACTGT GGAAGAAATT 
ATTGAAAACT TACAGCAAGA TGGTTCATCT TTTGCCCTAG AGCAATTGAA GGTAATTAAT 
AAAATGTCTC CAACATCTCT AAAGATCACA CTAAGGCAAC TCATGGAGGG GTCTTCAAAG 
ACCTTGCAAG AAGTACTAAC TATGGAGTAT CGGCTAAGTC AAGCTTGTAT GTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIBCH ( NM_198047.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_932164.1 Homo sapiens 3-hydroxyisobutyryl-CoA hydrolase (HIBCH), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu15155
Clone ID Related Accession (Same CDS sequence)	NM_198047.3 , NM_198047.2
Accession Version	NM_198047.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550678400000
Organism	Homo sapiens(human)
Product	3-hydroxyisobutyryl-CoA hydrolase, mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP193393.1, DB034438.1, AC092178.4, BC005190.1 and AK223023.1. On Mar 27, 2010 this sequence version replaced NM_198047.1. Transcript Variant: This variant (2) lacks an exon in the 3' coding region, which results in a frameshift and an early stop codon, compared to variant 1. The encoded isoform (2) has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.102143.1, SRR1163655.103985.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGCAGC GCGAGATGTG GAGGCTCATG TCGAGGTTTA ATGCATTCAA AAGGACTAAT 
ACCATACTGC ACCATTTGAG AATGTCCAAG CACACAGATG CAGCAGAAGA GGTGCTATTG 
GAAAAAAAAG GTTGCACGGG AGTCATAACA CTAAACAGAC CAAAGTTCCT CAATGCACTG 
ACTCTTAATA TGATTCGGCA GATTTATCCA CAGCTAAAGA AGTGGGAACA AGATCCTGAA 
ACTTTCCTGA TCATTATAAA GGGAGCAGGA GGAAAGGCTT TCTGTGCCGG GGGTGATATC 
AGAGTGATCT CGGAAGCTGA AAAGGCAAAA CAGAAGATAG CTCCAGTTTT CTTCAGAGAA 
GAATATATGC TGAATAATGC TGTTGGTTCT TGCCAGAAAC CTTATGTTGC ACTTATTCAT 
GGAATTACAA TGGGTGGGGG AGTTGGTCTC TCAGTCCATG GGCAATTTCG AGTGGCTACA 
GAAAAGTGTC TTTTTGCTAT GCCAGAAACT GCAATAGGAC TGTTCCCTGA TGTGGGTGGA 
GGTTATTTCT TGCCACGACT CCAAGGAAAA CTTGGTTACT TCCTTGCATT AACAGGATTC 
AGACTAAAAG GAAGAGATGT GTACAGAGCA GGAATTGCTA CACACTTTGT AGATTCTGAA 
AAGTTGGCCA TGTTAGAGGA AGATTTGTTA GCCTTGAAAT CTCCTTCAAA AGAAAATATT 
GCATCTGTCT TAGAAAATTA CCATACAGAG TCTAAGATTG ATCGAGACAA GTCTTTTATA 
CTTGAGGAAC ACATGGACAA AATAAACAGT TGTTTTTCAG CCAATACTGT GGAAGAAATT 
ATTGAAAACT TACAGCAAGA TGGTTCATCT TTTGCCCTAG AGCAATTGAA GGTAATTAAT 
AAAATGTCTC CAACATCTCT AAAGATCACA CTAAGGCAAC TCATGGAGGG GTCTTCAAAG 
ACCTTGCAAG AAGTACTAAC TATGGAGTAT CGGCTAAGTC AAGCTTGTAT GTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_932164.1
CDS	296..1312
Translation	MGQREMWRLMSRFNAFKRTNTILHHLRMSKHTDAAEEVLLEKKGCTGVITLNRPKFLNALTLNMIRQIYPQLKKWEQDPETFLIIIKGAGGKAFCAGGDIRVISEAEKAKQKIAPVFFREEYMLNNAVGSCQKPYVALIHGITMGGGVGLSVHGQFRVATEKCLFAMPETAIGLFPDVGGGYFLPRLQGKLGYFLALTGFRLKGRDVYRAGIATHFVDSEKLAMLEEDLLALKSPSKENIASVLENYHTESKIDRDKSFILEEHMDKINSCFSANTVEEIIENLQQDGSSFALEQLKVINKMSPTSLKITLRQLMEGSSKTLQEVLTMEYRLSQACMF

Target ORF information:

RefSeq Version	NM_198047.2
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxyisobutyryl-CoA hydrolase (HIBCH), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198047.2

ORF Insert Sequence:

ATGGGGCAGC GCGAGATGTG GAGGCTCATG TCGAGGTTTA ATGCATTCAA AAGGACTAAT 
ACCATACTGC ACCATTTGAG AATGTCCAAG CACACAGATG CAGCAGAAGA GGTGCTATTG 
GAAAAAAAAG GTTGCACGGG AGTCATAACA CTAAACAGAC CAAAGTTCCT CAATGCACTG 
ACTCTTAATA TGATTCGGCA GATTTATCCA CAGCTAAAGA AGTGGGAACA AGATCCTGAA 
ACTTTCCTGA TCATTATAAA GGGAGCAGGA GGAAAGGCTT TCTGTGCCGG GGGTGATATC 
AGAGTGATCT CGGAAGCTGA AAAGGCAAAA CAGAAGATAG CTCCAGTTTT CTTCAGAGAA 
GAATATATGC TGAATAATGC TGTTGGTTCT TGCCAGAAAC CTTATGTTGC ACTTATTCAT 
GGAATTACAA TGGGTGGGGG AGTTGGTCTC TCAGTCCATG GGCAATTTCG AGTGGCTACA 
GAAAAGTGTC TTTTTGCTAT GCCAGAAACT GCAATAGGAC TGTTCCCTGA TGTGGGTGGA 
GGTTATTTCT TGCCACGACT CCAAGGAAAA CTTGGTTACT TCCTTGCATT AACAGGATTC 
AGACTAAAAG GAAGAGATGT GTACAGAGCA GGAATTGCTA CACACTTTGT AGATTCTGAA 
AAGTTGGCCA TGTTAGAGGA AGATTTGTTA GCCTTGAAAT CTCCTTCAAA AGAAAATATT 
GCATCTGTCT TAGAAAATTA CCATACAGAG TCTAAGATTG ATCGAGACAA GTCTTTTATA 
CTTGAGGAAC ACATGGACAA AATAAACAGT TGTTTTTCAG CCAATACTGT GGAAGAAATT 
ATTGAAAACT TACAGCAAGA TGGTTCATCT TTTGCCCTAG AGCAATTGAA GGTAATTAAT 
AAAATGTCTC CAACATCTCT AAAGATCACA CTAAGGCAAC TCATGGAGGG GTCTTCAAAG 
ACCTTGCAAG AAGTACTAAC TATGGAGTAT CGGCTAAGTC AAGCTTGTAT GTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIBCH ( XM_011510954.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509256.1 Homo sapiens 3-hydroxyisobutyryl-CoA hydrolase (HIBCH), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu66919
Clone ID Related Accession (Same CDS sequence)	XM_011510954.1
Accession Version	XM_011510954.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	3-hydroxyisobutyryl-CoA hydrolase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGAAA CTGCAATAGG ACTGTTCCCT GATGTGGGTG GAGGTTATTT CTTGCCACGA 
CTCCAAGGAA AACTTGGTTA CTTCCTTGCA TTAACAGGAT TCAGACTAAA AGGAAGAGAT 
GTGTACAGAG CAGGAATTGC TACACACTTT GTAGATTCTG AAAAGTTGGC CATGTTAGAG 
GAAGATTTGT TAGCCTTGAA ATCTCCTTCA AAAGAAAATA TTGCATCTGT CTTAGAAAAT 
TACCATACAG AGTCTAAGAT TGATCGAGAC AAGTCTTTTA TACTTGAGGA ACACATGGAC 
AAAATAAACA GTTGTTTTTC AGCCAATACT GTGGAAGAAA TTATTGAAAA CTTACAGCAA 
GATGGTTCAT CTTTTGCCCT AGAGCAATTG AAGGTAATTA ATAAAATGTC TCCAACATCT 
CTAAAGATCA CACTAAGGCA ACTCATGGAG GGGTCTTCAA AGACCTTGCA AGAAGTACTA 
ACTATGGAGT ATCGGCTAAG TCAAGCTTGT ATGAGAGGTC ATGACTTTCA TGAAGGCGTT 
AGAGCTGTTT TAATTGATAA AGACCAGAGT CCAAAATGGA AACCAGCTGA TCTAAAAGAA 
GTTACTGAGG AAGATTTGAA TAATCACTTT AAGTCTTTGG GAAGCAGTGA TTTGAAATTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509256.1
CDS	194..856
Translation	MPETAIGLFPDVGGGYFLPRLQGKLGYFLALTGFRLKGRDVYRAGIATHFVDSEKLAMLEEDLLALKSPSKENIASVLENYHTESKIDRDKSFILEEHMDKINSCFSANTVEEIIENLQQDGSSFALEQLKVINKMSPTSLKITLRQLMEGSSKTLQEVLTMEYRLSQACMRGHDFHEGVRAVLIDKDQSPKWKPADLKEVTEEDLNNHFKSLGSSDLKF

Target ORF information:

RefSeq Version	XM_011510954.1
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxyisobutyryl-CoA hydrolase (HIBCH), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510954.1

ORF Insert Sequence:

ATGCCAGAAA CTGCAATAGG ACTGTTCCCT GATGTGGGTG GAGGTTATTT CTTGCCACGA 
CTCCAAGGAA AACTTGGTTA CTTCCTTGCA TTAACAGGAT TCAGACTAAA AGGAAGAGAT 
GTGTACAGAG CAGGAATTGC TACACACTTT GTAGATTCTG AAAAGTTGGC CATGTTAGAG 
GAAGATTTGT TAGCCTTGAA ATCTCCTTCA AAAGAAAATA TTGCATCTGT CTTAGAAAAT 
TACCATACAG AGTCTAAGAT TGATCGAGAC AAGTCTTTTA TACTTGAGGA ACACATGGAC 
AAAATAAACA GTTGTTTTTC AGCCAATACT GTGGAAGAAA TTATTGAAAA CTTACAGCAA 
GATGGTTCAT CTTTTGCCCT AGAGCAATTG AAGGTAATTA ATAAAATGTC TCCAACATCT 
CTAAAGATCA CACTAAGGCA ACTCATGGAG GGGTCTTCAA AGACCTTGCA AGAAGTACTA 
ACTATGGAGT ATCGGCTAAG TCAAGCTTGT ATGAGAGGTC ATGACTTTCA TGAAGGCGTT 
AGAGCTGTTT TAATTGATAA AGACCAGAGT CCAAAATGGA AACCAGCTGA TCTAAAAGAA 
GTTACTGAGG AAGATTTGAA TAATCACTTT AAGTCTTTGG GAAGCAGTGA TTTGAAATTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIBCH ( NM_014362.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055177.2 Homo sapiens 3-hydroxyisobutyryl-CoA hydrolase (HIBCH), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23117
Clone ID Related Accession (Same CDS sequence)	XM_011510953.2 , NM_014362.4 , NM_014362.3 , XM_011510953.1
Accession Version	NM_014362.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577548800000
Organism	Homo sapiens(human)
Product	3-hydroxyisobutyryl-CoA hydrolase, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092178.4, AC010679.6 and AK223023.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_014362.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660803.42595.1, SRR1660803.57272.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000359678.10/ ENSP00000352706.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGCAGC GCGAGATGTG GAGGCTCATG TCGAGGTTTA ATGCATTCAA AAGGACTAAT 
ACCATACTGC ACCATTTGAG AATGTCCAAG CACACAGATG CAGCAGAAGA GGTGCTATTG 
GAAAAAAAAG GTTGCACGGG AGTCATAACA CTAAACAGAC CAAAGTTCCT CAATGCACTG 
ACTCTTAATA TGATTCGGCA GATTTATCCA CAGCTAAAGA AGTGGGAACA AGATCCTGAA 
ACTTTCCTGA TCATTATAAA GGGAGCAGGA GGAAAGGCTT TCTGTGCCGG GGGTGATATC 
AGAGTGATCT CGGAAGCTGA AAAGGCAAAA CAGAAGATAG CTCCAGTTTT CTTCAGAGAA 
GAATATATGC TGAATAATGC TGTTGGTTCT TGCCAGAAAC CTTATGTTGC ACTTATTCAT 
GGAATTACAA TGGGTGGGGG AGTTGGTCTC TCAGTCCATG GGCAATTTCG AGTGGCTACA 
GAAAAGTGTC TTTTTGCTAT GCCAGAAACT GCAATAGGAC TGTTCCCTGA TGTGGGTGGA 
GGTTATTTCT TGCCACGACT CCAAGGAAAA CTTGGTTACT TCCTTGCATT AACAGGATTC 
AGACTAAAAG GAAGAGATGT GTACAGAGCA GGAATTGCTA CACACTTTGT AGATTCTGAA 
AAGTTGGCCA TGTTAGAGGA AGATTTGTTA GCCTTGAAAT CTCCTTCAAA AGAAAATATT 
GCATCTGTCT TAGAAAATTA CCATACAGAG TCTAAGATTG ATCGAGACAA GTCTTTTATA 
CTTGAGGAAC ACATGGACAA AATAAACAGT TGTTTTTCAG CCAATACTGT GGAAGAAATT 
ATTGAAAACT TACAGCAAGA TGGTTCATCT TTTGCCCTAG AGCAATTGAA GGTAATTAAT 
AAAATGTCTC CAACATCTCT AAAGATCACA CTAAGGCAAC TCATGGAGGG GTCTTCAAAG 
ACCTTGCAAG AAGTACTAAC TATGGAGTAT CGGCTAAGTC AAGCTTGTAT GAGAGGTCAT 
GACTTTCATG AAGGCGTTAG AGCTGTTTTA ATTGATAAAG ACCAGAGTCC AAAATGGAAA 
CCAGCTGATC TAAAAGAAGT TACTGAGGAA GATTTGAATA ATCACTTTAA GTCTTTGGGA 
AGCAGTGATT TGAAATTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055177.2
CDS	77..1237
Translation	MGQREMWRLMSRFNAFKRTNTILHHLRMSKHTDAAEEVLLEKKGCTGVITLNRPKFLNALTLNMIRQIYPQLKKWEQDPETFLIIIKGAGGKAFCAGGDIRVISEAEKAKQKIAPVFFREEYMLNNAVGSCQKPYVALIHGITMGGGVGLSVHGQFRVATEKCLFAMPETAIGLFPDVGGGYFLPRLQGKLGYFLALTGFRLKGRDVYRAGIATHFVDSEKLAMLEEDLLALKSPSKENIASVLENYHTESKIDRDKSFILEEHMDKINSCFSANTVEEIIENLQQDGSSFALEQLKVINKMSPTSLKITLRQLMEGSSKTLQEVLTMEYRLSQACMRGHDFHEGVRAVLIDKDQSPKWKPADLKEVTEEDLNNHFKSLGSSDLKF

Target ORF information:

RefSeq Version	NM_014362.4
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxyisobutyryl-CoA hydrolase (HIBCH), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014362.4

ORF Insert Sequence:

ATGGGGCAGC GCGAGATGTG GAGGCTCATG TCGAGGTTTA ATGCATTCAA AAGGACTAAT 
ACCATACTGC ACCATTTGAG AATGTCCAAG CACACAGATG CAGCAGAAGA GGTGCTATTG 
GAAAAAAAAG GTTGCACGGG AGTCATAACA CTAAACAGAC CAAAGTTCCT CAATGCACTG 
ACTCTTAATA TGATTCGGCA GATTTATCCA CAGCTAAAGA AGTGGGAACA AGATCCTGAA 
ACTTTCCTGA TCATTATAAA GGGAGCAGGA GGAAAGGCTT TCTGTGCCGG GGGTGATATC 
AGAGTGATCT CGGAAGCTGA AAAGGCAAAA CAGAAGATAG CTCCAGTTTT CTTCAGAGAA 
GAATATATGC TGAATAATGC TGTTGGTTCT TGCCAGAAAC CTTATGTTGC ACTTATTCAT 
GGAATTACAA TGGGTGGGGG AGTTGGTCTC TCAGTCCATG GGCAATTTCG AGTGGCTACA 
GAAAAGTGTC TTTTTGCTAT GCCAGAAACT GCAATAGGAC TGTTCCCTGA TGTGGGTGGA 
GGTTATTTCT TGCCACGACT CCAAGGAAAA CTTGGTTACT TCCTTGCATT AACAGGATTC 
AGACTAAAAG GAAGAGATGT GTACAGAGCA GGAATTGCTA CACACTTTGT AGATTCTGAA 
AAGTTGGCCA TGTTAGAGGA AGATTTGTTA GCCTTGAAAT CTCCTTCAAA AGAAAATATT 
GCATCTGTCT TAGAAAATTA CCATACAGAG TCTAAGATTG ATCGAGACAA GTCTTTTATA 
CTTGAGGAAC ACATGGACAA AATAAACAGT TGTTTTTCAG CCAATACTGT GGAAGAAATT 
ATTGAAAACT TACAGCAAGA TGGTTCATCT TTTGCCCTAG AGCAATTGAA GGTAATTAAT 
AAAATGTCTC CAACATCTCT AAAGATCACA CTAAGGCAAC TCATGGAGGG GTCTTCAAAG 
ACCTTGCAAG AAGTACTAAC TATGGAGTAT CGGCTAAGTC AAGCTTGTAT GAGAGGTCAT 
GACTTTCATG AAGGCGTTAG AGCTGTTTTA ATTGATAAAG ACCAGAGTCC AAAATGGAAA 
CCAGCTGATC TAAAAGAAGT TACTGAGGAA GATTTGAATA ATCACTTTAA GTCTTTGGGA 
AGCAGTGATT TGAAATTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIBCH ( NM_014362.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055177.2 Homo sapiens 3-hydroxyisobutyryl-CoA hydrolase (HIBCH), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23117
Clone ID Related Accession (Same CDS sequence)	XM_011510953.2 , NM_014362.4 , NM_014362.3 , XM_011510953.1
Accession Version	NM_014362.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1514649600000
Organism	Homo sapiens(human)
Product	3-hydroxyisobutyryl-CoA hydrolase, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP193393.1, DB034438.1, AC092178.4, AC010679.6 and AK223023.1. This sequence is a reference standard in the RefSeqGene project. On Mar 27, 2010 this sequence version replaced NM_014362.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK222979.1, SRR1660809.187695.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGCAGC GCGAGATGTG GAGGCTCATG TCGAGGTTTA ATGCATTCAA AAGGACTAAT 
ACCATACTGC ACCATTTGAG AATGTCCAAG CACACAGATG CAGCAGAAGA GGTGCTATTG 
GAAAAAAAAG GTTGCACGGG AGTCATAACA CTAAACAGAC CAAAGTTCCT CAATGCACTG 
ACTCTTAATA TGATTCGGCA GATTTATCCA CAGCTAAAGA AGTGGGAACA AGATCCTGAA 
ACTTTCCTGA TCATTATAAA GGGAGCAGGA GGAAAGGCTT TCTGTGCCGG GGGTGATATC 
AGAGTGATCT CGGAAGCTGA AAAGGCAAAA CAGAAGATAG CTCCAGTTTT CTTCAGAGAA 
GAATATATGC TGAATAATGC TGTTGGTTCT TGCCAGAAAC CTTATGTTGC ACTTATTCAT 
GGAATTACAA TGGGTGGGGG AGTTGGTCTC TCAGTCCATG GGCAATTTCG AGTGGCTACA 
GAAAAGTGTC TTTTTGCTAT GCCAGAAACT GCAATAGGAC TGTTCCCTGA TGTGGGTGGA 
GGTTATTTCT TGCCACGACT CCAAGGAAAA CTTGGTTACT TCCTTGCATT AACAGGATTC 
AGACTAAAAG GAAGAGATGT GTACAGAGCA GGAATTGCTA CACACTTTGT AGATTCTGAA 
AAGTTGGCCA TGTTAGAGGA AGATTTGTTA GCCTTGAAAT CTCCTTCAAA AGAAAATATT 
GCATCTGTCT TAGAAAATTA CCATACAGAG TCTAAGATTG ATCGAGACAA GTCTTTTATA 
CTTGAGGAAC ACATGGACAA AATAAACAGT TGTTTTTCAG CCAATACTGT GGAAGAAATT 
ATTGAAAACT TACAGCAAGA TGGTTCATCT TTTGCCCTAG AGCAATTGAA GGTAATTAAT 
AAAATGTCTC CAACATCTCT AAAGATCACA CTAAGGCAAC TCATGGAGGG GTCTTCAAAG 
ACCTTGCAAG AAGTACTAAC TATGGAGTAT CGGCTAAGTC AAGCTTGTAT GAGAGGTCAT 
GACTTTCATG AAGGCGTTAG AGCTGTTTTA ATTGATAAAG ACCAGAGTCC AAAATGGAAA 
CCAGCTGATC TAAAAGAAGT TACTGAGGAA GATTTGAATA ATCACTTTAA GTCTTTGGGA 
AGCAGTGATT TGAAATTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055177.2
CDS	296..1456
Translation	MGQREMWRLMSRFNAFKRTNTILHHLRMSKHTDAAEEVLLEKKGCTGVITLNRPKFLNALTLNMIRQIYPQLKKWEQDPETFLIIIKGAGGKAFCAGGDIRVISEAEKAKQKIAPVFFREEYMLNNAVGSCQKPYVALIHGITMGGGVGLSVHGQFRVATEKCLFAMPETAIGLFPDVGGGYFLPRLQGKLGYFLALTGFRLKGRDVYRAGIATHFVDSEKLAMLEEDLLALKSPSKENIASVLENYHTESKIDRDKSFILEEHMDKINSCFSANTVEEIIENLQQDGSSFALEQLKVINKMSPTSLKITLRQLMEGSSKTLQEVLTMEYRLSQACMRGHDFHEGVRAVLIDKDQSPKWKPADLKEVTEEDLNNHFKSLGSSDLKF

Target ORF information:

RefSeq Version	NM_014362.3
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxyisobutyryl-CoA hydrolase (HIBCH), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014362.3

ORF Insert Sequence:

ATGGGGCAGC GCGAGATGTG GAGGCTCATG TCGAGGTTTA ATGCATTCAA AAGGACTAAT 
ACCATACTGC ACCATTTGAG AATGTCCAAG CACACAGATG CAGCAGAAGA GGTGCTATTG 
GAAAAAAAAG GTTGCACGGG AGTCATAACA CTAAACAGAC CAAAGTTCCT CAATGCACTG 
ACTCTTAATA TGATTCGGCA GATTTATCCA CAGCTAAAGA AGTGGGAACA AGATCCTGAA 
ACTTTCCTGA TCATTATAAA GGGAGCAGGA GGAAAGGCTT TCTGTGCCGG GGGTGATATC 
AGAGTGATCT CGGAAGCTGA AAAGGCAAAA CAGAAGATAG CTCCAGTTTT CTTCAGAGAA 
GAATATATGC TGAATAATGC TGTTGGTTCT TGCCAGAAAC CTTATGTTGC ACTTATTCAT 
GGAATTACAA TGGGTGGGGG AGTTGGTCTC TCAGTCCATG GGCAATTTCG AGTGGCTACA 
GAAAAGTGTC TTTTTGCTAT GCCAGAAACT GCAATAGGAC TGTTCCCTGA TGTGGGTGGA 
GGTTATTTCT TGCCACGACT CCAAGGAAAA CTTGGTTACT TCCTTGCATT AACAGGATTC 
AGACTAAAAG GAAGAGATGT GTACAGAGCA GGAATTGCTA CACACTTTGT AGATTCTGAA 
AAGTTGGCCA TGTTAGAGGA AGATTTGTTA GCCTTGAAAT CTCCTTCAAA AGAAAATATT 
GCATCTGTCT TAGAAAATTA CCATACAGAG TCTAAGATTG ATCGAGACAA GTCTTTTATA 
CTTGAGGAAC ACATGGACAA AATAAACAGT TGTTTTTCAG CCAATACTGT GGAAGAAATT 
ATTGAAAACT TACAGCAAGA TGGTTCATCT TTTGCCCTAG AGCAATTGAA GGTAATTAAT 
AAAATGTCTC CAACATCTCT AAAGATCACA CTAAGGCAAC TCATGGAGGG GTCTTCAAAG 
ACCTTGCAAG AAGTACTAAC TATGGAGTAT CGGCTAAGTC AAGCTTGTAT GAGAGGTCAT 
GACTTTCATG AAGGCGTTAG AGCTGTTTTA ATTGATAAAG ACCAGAGTCC AAAATGGAAA 
CCAGCTGATC TAAAAGAAGT TACTGAGGAA GATTTGAATA ATCACTTTAA GTCTTTGGGA 
AGCAGTGATT TGAAATTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIBCH ( XM_011510953.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509255.1 Homo sapiens 3-hydroxyisobutyryl-CoA hydrolase (HIBCH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu23117
Clone ID Related Accession (Same CDS sequence)	XM_011510953.2 , NM_014362.4 , NM_014362.3 , XM_011510953.1
Accession Version	XM_011510953.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	3-hydroxyisobutyryl-CoA hydrolase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011510953.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAGC GCGAGATGTG GAGGCTCATG TCGAGGTTTA ATGCATTCAA AAGGACTAAT 
ACCATACTGC ACCATTTGAG AATGTCCAAG CACACAGATG CAGCAGAAGA GGTGCTATTG 
GAAAAAAAAG GTTGCACGGG AGTCATAACA CTAAACAGAC CAAAGTTCCT CAATGCACTG 
ACTCTTAATA TGATTCGGCA GATTTATCCA CAGCTAAAGA AGTGGGAACA AGATCCTGAA 
ACTTTCCTGA TCATTATAAA GGGAGCAGGA GGAAAGGCTT TCTGTGCCGG GGGTGATATC 
AGAGTGATCT CGGAAGCTGA AAAGGCAAAA CAGAAGATAG CTCCAGTTTT CTTCAGAGAA 
GAATATATGC TGAATAATGC TGTTGGTTCT TGCCAGAAAC CTTATGTTGC ACTTATTCAT 
GGAATTACAA TGGGTGGGGG AGTTGGTCTC TCAGTCCATG GGCAATTTCG AGTGGCTACA 
GAAAAGTGTC TTTTTGCTAT GCCAGAAACT GCAATAGGAC TGTTCCCTGA TGTGGGTGGA 
GGTTATTTCT TGCCACGACT CCAAGGAAAA CTTGGTTACT TCCTTGCATT AACAGGATTC 
AGACTAAAAG GAAGAGATGT GTACAGAGCA GGAATTGCTA CACACTTTGT AGATTCTGAA 
AAGTTGGCCA TGTTAGAGGA AGATTTGTTA GCCTTGAAAT CTCCTTCAAA AGAAAATATT 
GCATCTGTCT TAGAAAATTA CCATACAGAG TCTAAGATTG ATCGAGACAA GTCTTTTATA 
CTTGAGGAAC ACATGGACAA AATAAACAGT TGTTTTTCAG CCAATACTGT GGAAGAAATT 
ATTGAAAACT TACAGCAAGA TGGTTCATCT TTTGCCCTAG AGCAATTGAA GGTAATTAAT 
AAAATGTCTC CAACATCTCT AAAGATCACA CTAAGGCAAC TCATGGAGGG GTCTTCAAAG 
ACCTTGCAAG AAGTACTAAC TATGGAGTAT CGGCTAAGTC AAGCTTGTAT GAGAGGTCAT 
GACTTTCATG AAGGCGTTAG AGCTGTTTTA ATTGATAAAG ACCAGAGTCC AAAATGGAAA 
CCAGCTGATC TAAAAGAAGT TACTGAGGAA GATTTGAATA ATCACTTTAA GTCTTTGGGA 
AGCAGTGATT TGAAATTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509255.1
CDS	64..1224
Translation	MGQREMWRLMSRFNAFKRTNTILHHLRMSKHTDAAEEVLLEKKGCTGVITLNRPKFLNALTLNMIRQIYPQLKKWEQDPETFLIIIKGAGGKAFCAGGDIRVISEAEKAKQKIAPVFFREEYMLNNAVGSCQKPYVALIHGITMGGGVGLSVHGQFRVATEKCLFAMPETAIGLFPDVGGGYFLPRLQGKLGYFLALTGFRLKGRDVYRAGIATHFVDSEKLAMLEEDLLALKSPSKENIASVLENYHTESKIDRDKSFILEEHMDKINSCFSANTVEEIIENLQQDGSSFALEQLKVINKMSPTSLKITLRQLMEGSSKTLQEVLTMEYRLSQACMRGHDFHEGVRAVLIDKDQSPKWKPADLKEVTEEDLNNHFKSLGSSDLKF

Target ORF information:

RefSeq Version	XM_011510953.2
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxyisobutyryl-CoA hydrolase (HIBCH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510953.2

ORF Insert Sequence:

ATGGGGCAGC GCGAGATGTG GAGGCTCATG TCGAGGTTTA ATGCATTCAA AAGGACTAAT 
ACCATACTGC ACCATTTGAG AATGTCCAAG CACACAGATG CAGCAGAAGA GGTGCTATTG 
GAAAAAAAAG GTTGCACGGG AGTCATAACA CTAAACAGAC CAAAGTTCCT CAATGCACTG 
ACTCTTAATA TGATTCGGCA GATTTATCCA CAGCTAAAGA AGTGGGAACA AGATCCTGAA 
ACTTTCCTGA TCATTATAAA GGGAGCAGGA GGAAAGGCTT TCTGTGCCGG GGGTGATATC 
AGAGTGATCT CGGAAGCTGA AAAGGCAAAA CAGAAGATAG CTCCAGTTTT CTTCAGAGAA 
GAATATATGC TGAATAATGC TGTTGGTTCT TGCCAGAAAC CTTATGTTGC ACTTATTCAT 
GGAATTACAA TGGGTGGGGG AGTTGGTCTC TCAGTCCATG GGCAATTTCG AGTGGCTACA 
GAAAAGTGTC TTTTTGCTAT GCCAGAAACT GCAATAGGAC TGTTCCCTGA TGTGGGTGGA 
GGTTATTTCT TGCCACGACT CCAAGGAAAA CTTGGTTACT TCCTTGCATT AACAGGATTC 
AGACTAAAAG GAAGAGATGT GTACAGAGCA GGAATTGCTA CACACTTTGT AGATTCTGAA 
AAGTTGGCCA TGTTAGAGGA AGATTTGTTA GCCTTGAAAT CTCCTTCAAA AGAAAATATT 
GCATCTGTCT TAGAAAATTA CCATACAGAG TCTAAGATTG ATCGAGACAA GTCTTTTATA 
CTTGAGGAAC ACATGGACAA AATAAACAGT TGTTTTTCAG CCAATACTGT GGAAGAAATT 
ATTGAAAACT TACAGCAAGA TGGTTCATCT TTTGCCCTAG AGCAATTGAA GGTAATTAAT 
AAAATGTCTC CAACATCTCT AAAGATCACA CTAAGGCAAC TCATGGAGGG GTCTTCAAAG 
ACCTTGCAAG AAGTACTAAC TATGGAGTAT CGGCTAAGTC AAGCTTGTAT GAGAGGTCAT 
GACTTTCATG AAGGCGTTAG AGCTGTTTTA ATTGATAAAG ACCAGAGTCC AAAATGGAAA 
CCAGCTGATC TAAAAGAAGT TACTGAGGAA GATTTGAATA ATCACTTTAA GTCTTTGGGA 
AGCAGTGATT TGAAATTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIBCH ( XM_011510953.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509255.1 Homo sapiens 3-hydroxyisobutyryl-CoA hydrolase (HIBCH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu23117
Clone ID Related Accession (Same CDS sequence)	XM_011510953.2 , NM_014362.4 , NM_014362.3 , XM_011510953.1
Accession Version	XM_011510953.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	3-hydroxyisobutyryl-CoA hydrolase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAGC GCGAGATGTG GAGGCTCATG TCGAGGTTTA ATGCATTCAA AAGGACTAAT 
ACCATACTGC ACCATTTGAG AATGTCCAAG CACACAGATG CAGCAGAAGA GGTGCTATTG 
GAAAAAAAAG GTTGCACGGG AGTCATAACA CTAAACAGAC CAAAGTTCCT CAATGCACTG 
ACTCTTAATA TGATTCGGCA GATTTATCCA CAGCTAAAGA AGTGGGAACA AGATCCTGAA 
ACTTTCCTGA TCATTATAAA GGGAGCAGGA GGAAAGGCTT TCTGTGCCGG GGGTGATATC 
AGAGTGATCT CGGAAGCTGA AAAGGCAAAA CAGAAGATAG CTCCAGTTTT CTTCAGAGAA 
GAATATATGC TGAATAATGC TGTTGGTTCT TGCCAGAAAC CTTATGTTGC ACTTATTCAT 
GGAATTACAA TGGGTGGGGG AGTTGGTCTC TCAGTCCATG GGCAATTTCG AGTGGCTACA 
GAAAAGTGTC TTTTTGCTAT GCCAGAAACT GCAATAGGAC TGTTCCCTGA TGTGGGTGGA 
GGTTATTTCT TGCCACGACT CCAAGGAAAA CTTGGTTACT TCCTTGCATT AACAGGATTC 
AGACTAAAAG GAAGAGATGT GTACAGAGCA GGAATTGCTA CACACTTTGT AGATTCTGAA 
AAGTTGGCCA TGTTAGAGGA AGATTTGTTA GCCTTGAAAT CTCCTTCAAA AGAAAATATT 
GCATCTGTCT TAGAAAATTA CCATACAGAG TCTAAGATTG ATCGAGACAA GTCTTTTATA 
CTTGAGGAAC ACATGGACAA AATAAACAGT TGTTTTTCAG CCAATACTGT GGAAGAAATT 
ATTGAAAACT TACAGCAAGA TGGTTCATCT TTTGCCCTAG AGCAATTGAA GGTAATTAAT 
AAAATGTCTC CAACATCTCT AAAGATCACA CTAAGGCAAC TCATGGAGGG GTCTTCAAAG 
ACCTTGCAAG AAGTACTAAC TATGGAGTAT CGGCTAAGTC AAGCTTGTAT GAGAGGTCAT 
GACTTTCATG AAGGCGTTAG AGCTGTTTTA ATTGATAAAG ACCAGAGTCC AAAATGGAAA 
CCAGCTGATC TAAAAGAAGT TACTGAGGAA GATTTGAATA ATCACTTTAA GTCTTTGGGA 
AGCAGTGATT TGAAATTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509255.1
CDS	245..1405
Translation	MGQREMWRLMSRFNAFKRTNTILHHLRMSKHTDAAEEVLLEKKGCTGVITLNRPKFLNALTLNMIRQIYPQLKKWEQDPETFLIIIKGAGGKAFCAGGDIRVISEAEKAKQKIAPVFFREEYMLNNAVGSCQKPYVALIHGITMGGGVGLSVHGQFRVATEKCLFAMPETAIGLFPDVGGGYFLPRLQGKLGYFLALTGFRLKGRDVYRAGIATHFVDSEKLAMLEEDLLALKSPSKENIASVLENYHTESKIDRDKSFILEEHMDKINSCFSANTVEEIIENLQQDGSSFALEQLKVINKMSPTSLKITLRQLMEGSSKTLQEVLTMEYRLSQACMRGHDFHEGVRAVLIDKDQSPKWKPADLKEVTEEDLNNHFKSLGSSDLKF

Target ORF information:

RefSeq Version	XM_011510953.1
Organism	Homo sapiens(human)
Definition	Homo sapiens 3-hydroxyisobutyryl-CoA hydrolase (HIBCH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011510953.1

ORF Insert Sequence:

ATGGGGCAGC GCGAGATGTG GAGGCTCATG TCGAGGTTTA ATGCATTCAA AAGGACTAAT 
ACCATACTGC ACCATTTGAG AATGTCCAAG CACACAGATG CAGCAGAAGA GGTGCTATTG 
GAAAAAAAAG GTTGCACGGG AGTCATAACA CTAAACAGAC CAAAGTTCCT CAATGCACTG 
ACTCTTAATA TGATTCGGCA GATTTATCCA CAGCTAAAGA AGTGGGAACA AGATCCTGAA 
ACTTTCCTGA TCATTATAAA GGGAGCAGGA GGAAAGGCTT TCTGTGCCGG GGGTGATATC 
AGAGTGATCT CGGAAGCTGA AAAGGCAAAA CAGAAGATAG CTCCAGTTTT CTTCAGAGAA 
GAATATATGC TGAATAATGC TGTTGGTTCT TGCCAGAAAC CTTATGTTGC ACTTATTCAT 
GGAATTACAA TGGGTGGGGG AGTTGGTCTC TCAGTCCATG GGCAATTTCG AGTGGCTACA 
GAAAAGTGTC TTTTTGCTAT GCCAGAAACT GCAATAGGAC TGTTCCCTGA TGTGGGTGGA 
GGTTATTTCT TGCCACGACT CCAAGGAAAA CTTGGTTACT TCCTTGCATT AACAGGATTC 
AGACTAAAAG GAAGAGATGT GTACAGAGCA GGAATTGCTA CACACTTTGT AGATTCTGAA 
AAGTTGGCCA TGTTAGAGGA AGATTTGTTA GCCTTGAAAT CTCCTTCAAA AGAAAATATT 
GCATCTGTCT TAGAAAATTA CCATACAGAG TCTAAGATTG ATCGAGACAA GTCTTTTATA 
CTTGAGGAAC ACATGGACAA AATAAACAGT TGTTTTTCAG CCAATACTGT GGAAGAAATT 
ATTGAAAACT TACAGCAAGA TGGTTCATCT TTTGCCCTAG AGCAATTGAA GGTAATTAAT 
AAAATGTCTC CAACATCTCT AAAGATCACA CTAAGGCAAC TCATGGAGGG GTCTTCAAAG 
ACCTTGCAAG AAGTACTAAC TATGGAGTAT CGGCTAAGTC AAGCTTGTAT GAGAGGTCAT 
GACTTTCATG AAGGCGTTAG AGCTGTTTTA ATTGATAAAG ACCAGAGTCC AAAATGGAAA 
CCAGCTGATC TAAAAGAAGT TACTGAGGAA GATTTGAATA ATCACTTTAA GTCTTTGGGA 
AGCAGTGATT TGAAATTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A movement disorder with dystonia and ataxia caused by a mutation in the HIBCH gene.
Movement disorders : official journal of the Movement Disorder Society31(11)1733-1739(2016 11)
Schottmann G,Sarpong A,Lorenz C,Weinhold N,Gill E,Teschner L,Ferdinandusse S,Wanders RJ,Prigione A,Schuelke M

A Common Polymorphism in HIBCH Influences Methylmalonic Acid Concentrations in Blood Independently of Cobalamin.
American journal of human genetics98(5)869-882(2016 May)
Molloy AM,Pangilinan F,Mills JL,Shane B,O'Neill MB,McGaughey DM,Velkova A,Abaan HO,Ueland PM,McNulty H,Ward M,Strain JJ,Cunningham C,Casey M,Cropp CD,Kim Y,Bailey-Wilson JE,Wilson AF,Brody LC

HIBCH mutations can cause Leigh-like disease with combined deficiency of multiple mitochondrial respiratory chain enzymes and pyruvate dehydrogenase.
Orphanet journal of rare diseases8188(2013 Dec)
Ferdinandusse S,Waterham HR,Heales SJ,Brown GK,Hargreaves IP,Taanman JW,Gunny R,Abulhoul L,Wanders RJ,Clayton PT,Leonard JV,Rahman S

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screening.
Proteomics7(11)1775-85(2007 Jun)
Wu C,Ma MH,Brown KR,Geisler M,Li L,Tzeng E,Jia CY,Jurisica I,Li SS

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HIBCH cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

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