GAST cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GAST
Entrez Gene ID	2520
Full Name	gastrin
Synonyms	GAS
General protein information	Preferred Names gastrin Names gastrin preprogastrin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q21.2
Summary	Gastrin is a hormone whose main function is to stimulate secretion of hydrochloric acid by the gastric mucosa, which results in gastrin formation inhibition. This hormone also acts as a mitogenic factor for gastrointestinal epithelial cells. Gastrin has two biologically active peptide forms, G34 and G17. [provided by RefSeq, Jul 2008].
Disorder MIM:	137250

mRNA and Protein(s)

mRNA	Protein	Name
NM_000805.5	NP_000796.1	gastrin preproprotein
NM_000805.4	NP_000796.1	gastrin preproprotein

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Gast	NP_034387.3
Bos taurus (cattle)	GAST	NP_776340.1
Homo sapiens (human)	GAST	NP_000796.1
Rattus norvegicus (Norway rat)	Gast	NP_036981.1
Canis lupus familiaris (dog)	GAST	XP_003435286.1
Pan troglodytes (chimpanzee)	GAST	XP_523651.2
Macaca mulatta (Rhesus monkey)	GAST	XP_001107333.1

Gene Ontology
Bibliography

Related articles in PubMed

Low serum gastrin associated with ER
Meng LL, Wang JL, Xu SP, Zu LD, Yan ZW, Zhang JB, Han YQ, Fu GH
BMC cancer18(1)824(2018 Aug)

Gastrin Protects Against Myocardial Ischemia/Reperfusion Injury via Activation of RISK (Reperfusion Injury Salvage Kinase) and SAFE (Survivor Activating Factor Enhancement) Pathways.
Yang X, Yue R, Zhang J, Zhang X, Liu Y, Chen C, Wang X, Luo H, Wang WE, Chen X, Wang HJ, Jose PA, Wang H, Zeng C
Journal of the American Heart Association7(14)(2018 07)

Critical role for non‑GAP function of Gαs in RGS1‑mediated promotion of melanoma progression through AKT and ERK phosphorylation.
Sun MY, Wang Y, Zhu J, Lv C, Wu K, Wang XW, Xue CY
Oncology reports39(6)2673-2680(2018 Jun)

Interrelation between ghrelin and gastrin in patients with combination of chronic gastritis and type 2 diabetes mellitus.
Sirchak ES, Patskun SV
Wiadomosci lekarskie (Warsaw, Poland : 1960)71(2 pt 1)311-314(2018)

[Expressions of gastrin and apoptosis-associated proteins involved in mitochondrial pathway in gastric cancer tissues and the clinical significance].
Feng L, Zhu J, Sun W, Zhao J, Liu Y
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology33(11)1557-1561(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GAST gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GAST cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu107224	NM_000805.5 Latest version!	Homo sapiens gastrin (GAST), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu55892	NM_000805.4 Latest version!	Homo sapiens gastrin (GAST), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GAST ( NM_000805.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000796.1 Homo sapiens gastrin (GAST), mRNA.

Price & Availability↑

CloneID	OHu107224
Clone ID Related Accession (Same CDS sequence)	NM_000805.5
Accession Version	NM_000805.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Homo sapiens(human)
Product	gastrin preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC130686.15 and AC109319.17. On Nov 22, 2018 this sequence version replaced NM_000805.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM768289.1, BM768748.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142586, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000329402.4/ ENSP00000331358.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGCGAC TGTGTGTGTA TGTGCTGATC TTTGCACTGG CTCTGGCCGC CTTCTCTGAA 
GCTTCTTGGA AGCCCCGCTC CCAGCAGCCA GATGCACCCT TAGGTACAGG GGCCAACAGG 
GACCTGGAGC TACCCTGGCT GGAGCAGCAG GGCCCAGCCT CTCATCATCG AAGGCAGCTG 
GGACCCCAGG GTCCCCCACA CCTCGTGGCA GACCCGTCCA AGAAGCAGGG ACCATGGCTG 
GAGGAAGAAG AAGAAGCCTA TGGATGGATG GACTTCGGCC GCCGCAGTGC TGAGGATGAG 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000796.1
CDS	63..368
Translation	MQRLCVYVLIFALALAAFSEASWKPRSQQPDAPLGTGANRDLELPWLEQQGPASHHRRQLGPQGPPHLVADPSKKQGPWLEEEEEAYGWMDFGRRSAEDEN

Target ORF information:

RefSeq Version	NM_000805.5
Organism	Homo sapiens(human)
Definition	Homo sapiens gastrin (GAST), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000805.5

ORF Insert Sequence:

ATGCAGCGAC TGTGTGTGTA TGTGCTGATC TTTGCACTGG CTCTGGCCGC CTTCTCTGAA 
GCTTCTTGGA AGCCCCGCTC CCAGCAGCCA GATGCACCCT TAGGTACAGG GGCCAACAGG 
GACCTGGAGC TACCCTGGCT GGAGCAGCAG GGCCCAGCCT CTCATCATCG AAGGCAGCTG 
GGACCCCAGG GTCCCCCACA CCTCGTGGCA GACCCGTCCA AGAAGCAGGG ACCATGGCTG 
GAGGAAGAAG AAGAAGCCTA TGGATGGATG GACTTCGGCC GCCGCAGTGC TGAGGATGAG 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAST ( NM_000805.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000796.1 Homo sapiens gastrin (GAST), mRNA.

Price & Availability↑

CloneID	OHu55892
Clone ID Related Accession (Same CDS sequence)	NM_000805.4
Accession Version	NM_000805.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1514390400000
Organism	Homo sapiens(human)
Product	gastrin preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM768762.1, BC069724.1 and BM768483.1. On Oct 15, 2011 this sequence version replaced NM_000805.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM854691.1, BM768762.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142586, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGCGAC TATGTGTGTA TGTGCTGATC TTTGCACTGG CTCTGGCCGC CTTCTCTGAA 
GCTTCTTGGA AGCCCCGCTC CCAGCAGCCA GATGCACCCT TAGGTACAGG GGCCAACAGG 
GACCTGGAGC TACCCTGGCT GGAGCAGCAG GGCCCAGCCT CTCATCATCG AAGGCAGCTG 
GGACCCCAGG GTCCCCCACA CCTCGTGGCA GACCCGTCCA AGAAGCAGGG ACCATGGCTG 
GAGGAAGAAG AAGAAGCCTA TGGATGGATG GACTTCGGCC GCCGCAGTGC TGAGGATGAG 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000796.1
CDS	68..373
Translation	MQRLCVYVLIFALALAAFSEASWKPRSQQPDAPLGTGANRDLELPWLEQQGPASHHRRQLGPQGPPHLVADPSKKQGPWLEEEEEAYGWMDFGRRSAEDEN

Target ORF information:

RefSeq Version	NM_000805.4
Organism	Homo sapiens(human)
Definition	Homo sapiens gastrin (GAST), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000805.4

ORF Insert Sequence:

ATGCAGCGAC TATGTGTGTA TGTGCTGATC TTTGCACTGG CTCTGGCCGC CTTCTCTGAA 
GCTTCTTGGA AGCCCCGCTC CCAGCAGCCA GATGCACCCT TAGGTACAGG GGCCAACAGG 
GACCTGGAGC TACCCTGGCT GGAGCAGCAG GGCCCAGCCT CTCATCATCG AAGGCAGCTG 
GGACCCCAGG GTCCCCCACA CCTCGTGGCA GACCCGTCCA AGAAGCAGGG ACCATGGCTG 
GAGGAAGAAG AAGAAGCCTA TGGATGGATG GACTTCGGCC GCCGCAGTGC TGAGGATGAG 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Low serum gastrin associated with ER
BMC cancer18(1)824(2018 Aug)
Meng LL,Wang JL,Xu SP,Zu LD,Yan ZW,Zhang JB,Han YQ,Fu GH

Gastrin Protects Against Myocardial Ischemia/Reperfusion Injury via Activation of RISK (Reperfusion Injury Salvage Kinase) and SAFE (Survivor Activating Factor Enhancement) Pathways.
Journal of the American Heart Association7(14)(2018 07)
Yang X,Yue R,Zhang J,Zhang X,Liu Y,Chen C,Wang X,Luo H,Wang WE,Chen X,Wang HJ,Jose PA,Wang H,Zeng C

Critical role for non‑GAP function of Gαs in RGS1‑mediated promotion of melanoma progression through AKT and ERK phosphorylation.
Oncology reports39(6)2673-2680(2018 Jun)
Sun MY,Wang Y,Zhu J,Lv C,Wu K,Wang XW,Xue CY

Interrelation between ghrelin and gastrin in patients with combination of chronic gastritis and type 2 diabetes mellitus.
Wiadomosci lekarskie (Warsaw, Poland : 1960)71(2 pt 1)311-314(2018)
Sirchak ES,Patskun SV

[Expressions of gastrin and apoptosis-associated proteins involved in mitochondrial pathway in gastric cancer tissues and the clinical significance].
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology33(11)1557-1561(2017 Nov)
Feng L,Zhu J,Sun W,Zhao J,Liu Y