TRIM35 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRIM35
Entrez Gene ID	23087
Full Name	tripartite motif containing 35
Synonyms	HLS5,MAIR
General protein information	Preferred Names tripartite motif containing 35 Names tripartite motif-containing protein 35 hemopoietic lineage switch protein 5
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8p21.2
Summary	The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The function of this protein has not been identified. [provided by RefSeq, Jul 2008].
Disorder MIM:	617007

mRNA and Protein(s)

mRNA	Protein	Name
NM_171982.5	NP_741983.2	tripartite motif-containing protein 35 isoform 1
NM_171982.4	NP_741983.2	tripartite motif-containing protein 35 isoform 1
XM_005273452.3	XP_005273509.1	tripartite motif-containing protein 35 isoform X2
NM_001362813.1	NP_001349742.1	tripartite motif-containing protein 35 isoform 3
NM_001362813.2	NP_001349742.1	tripartite motif-containing protein 35 isoform 3
NM_001304495.2	NP_001291424.1	tripartite motif-containing protein 35 isoform 2
XM_011544453.1	XP_011542755.1	tripartite motif-containing protein 35 isoform X1
NM_001304495.1	NP_001291424.1	tripartite motif-containing protein 35 isoform 2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	TRIM35	XP_001234476.2
Homo sapiens (human)	TRIM35	NP_741983.2
Pan troglodytes (chimpanzee)	TRIM35	XP_519674.2
Macaca mulatta (Rhesus monkey)	TRIM35	XP_001108814.1
Rattus norvegicus (Norway rat)	Trim35	NP_001020313.2
Canis lupus familiaris (dog)	TRIM35	XP_543229.3
Bos taurus (cattle)	TRIM35	XP_002689839.1
Mus musculus (house mouse)	Trim35	NP_084255.2

Gene Ontology
Bibliography

Related articles in PubMed

miR-4417 Targets Tripartite Motif-Containing 35 (TRIM35) and Regulates Pyruvate Kinase Muscle 2 (PKM2) Phosphorylation to Promote Proliferation and Suppress Apoptosis in Hepatocellular Carcinoma Cells.
Song L, Zhang W, Chang Z, Pan Y, Zong H, Fan Q, Wang L
Medical science monitor : international medical journal of experimental and clinical research231741-1750(2017 Apr)

TRIM35 Interacts with pyruvate kinase isoform M2 to suppress the Warburg effect and tumorigenicity in hepatocellular carcinoma.
Chen Z, Wang Z, Guo W, Zhang Z, Zhao F, Zhao Y, Jia D, Ding J, Wang H, Yao M, He X
Oncogene34(30)3946-56(2015 Jul)

Co-expression of PKM2 and TRIM35 predicts survival and recurrence in hepatocellular carcinoma.
Chen Z, Lu X, Wang Z, Jin G, Wang Q, Chen D, Chen T, Li J, Fan J, Cong W, Gao Q, He X
Oncotarget6(4)2538-48(2015 Feb)

TRIM E3 ligases interfere with early and late stages of the retroviral life cycle.
Uchil PD, Quinlan BD, Chan WT, Luna JM, Mothes W
PLoS pathogens4(2)e16(2008 Feb)

HLS5, a novel RBCC (ring finger, B box, coiled-coil) family member isolated from a hemopoietic lineage switch, is a candidate tumor suppressor.
Lalonde JP, Lim R, Ingley E, Tilbrook PA, Thompson MJ, McCulloch R, Beaumont JG, Wicking C, Eyre HJ, Sutherland GR, Howe K, Solomon E, Williams JH, Klinken SP
The Journal of biological chemistry279(9)8181-9(2004 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TRIM35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRIM35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu42024	NM_001362813.2 Latest version!	Homo sapiens tripartite motif containing 35 (TRIM35), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu42025	NM_001304495.2 Latest version!	Homo sapiens tripartite motif containing 35 (TRIM35), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu42024	XM_005273452.3 Latest version!	Homo sapiens tripartite motif containing 35 (TRIM35), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu65417	XM_011544453.1 Latest version!	Homo sapiens tripartite motif containing 35 (TRIM35), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu07109	NM_171982.5 Latest version!	Homo sapiens tripartite motif containing 35 (TRIM35), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
View Old Accession Versions >>
OHu07109	NM_171982.4	Homo sapiens tripartite motif containing 35 (TRIM35), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
OHu42024	NM_001362813.1	Homo sapiens tripartite motif containing 35 (TRIM35), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu42025	NM_001304495.1	Homo sapiens tripartite motif containing 35 (TRIM35), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRIM35 ( NM_001362813.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001349742.1 Homo sapiens tripartite motif containing 35 (TRIM35), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu42024
Clone ID Related Accession (Same CDS sequence)	XM_005273452.3 , NM_001362813.1 , NM_001362813.2
Accession Version	NM_001362813.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1551110400000
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 35 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124649.6, AF492463.1 and BU622420.1. On Apr 28, 2018 this sequence version replaced XM_005273452.3. Transcript Variant: This variant (3) lacks an alternate exon in the central coding region, resulting in a frameshift compared to variant 1. The encoded isoform (3) is shorter and has a distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.135832.1, SRR1803611.16580.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2161674 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCGGA GTCCCGACGT GTCCCCCGGG CCTTCCCGCT CCTTCAAGGA GGAGTTGCTC 
TGCGCCGTCT GCTACGACCC CTTCCGCGAC GCAGTCACTC TGCGCTGCGG CCACAACTTC 
TGCCGCGGGT GCGTGAGCCG CTGCTGGGAG GTGCAGGTGT CGCCCACCTG CCCAGTGTGC 
AAAGACCGCG CGTCACCCGC CGACCTGCGC ACCAACCACA CCCTCAACAA CCTGGTGGAG 
AAGCTGCTGC GCGAGGAGGC CGAGGGCGCG CGCTGGACCA GCTACCGCTT CTCGCGTGTC 
TGCCGCCTGC ACCGCGGACA GCTCAGCCTC TTCTGCCTCG AGGACAAGGA GCTGCTGTGC 
TGCTCCTGCC AGGCCGACCC CCGACACCAG GGGCACCGCG TGCAGCCGGT GAAGGACACT 
GCCCACGACT TTCGGGCCAA GTGCAGGAAC ATGGAGCATG CACTGCGGGA GAAGGCCAAG 
GCCTTCTGGG CCATGCGGCG CTCCTATGAG GCCATCGCCA AGCACAATCA GGTGGAGGCT 
GCATGGCTGG AAGGCCGGAT CCGGCAGGAG TTTGATAAGC TTCGCGAGTT CTTGAGAGTG 
GAGGAGCAGG CCATTCTGGA TGCCATGGCC GAGGAGACAA GGCAGAAGCA ACTTCTGGCC 
GACGAGAAGA TGAAGCAGCT CACAGAGGAG ACGGAGGTGC TGGCACATGA GATCGAGCGG 
CTGCAGATGG AGATGAAGGA GGACGACGTT TCTTTTCTCA TGACTCTTCT GCACCATGGA 
GCCAGAGCCA GTCCAGCCCG GCATGCTTAT CGATGTCTGC AAGTACCTGG GCTCCCTGCA 
GTACCGCGTC TGGAAGAAGA TGCTTGCATC TGTGGAATCT GTACCCTTCA GCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349742.1
CDS	85..981
Translation	MERSPDVSPGPSRSFKEELLCAVCYDPFRDAVTLRCGHNFCRGCVSRCWEVQVSPTCPVCKDRASPADLRTNHTLNNLVEKLLREEAEGARWTSYRFSRVCRLHRGQLSLFCLEDKELLCCSCQADPRHQGHRVQPVKDTAHDFRAKCRNMEHALREKAKAFWAMRRSYEAIAKHNQVEAAWLEGRIRQEFDKLREFLRVEEQAILDAMAEETRQKQLLADEKMKQLTEETEVLAHEIERLQMEMKEDDVSFLMTLLHHGARASPARHAYRCLQVPGLPAVPRLEEDACICGICTLQL

Target ORF information:

RefSeq Version	NM_001362813.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 35 (TRIM35), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362813.1

ORF Insert Sequence:

ATGGAGCGGA GTCCCGACGT GTCCCCCGGG CCTTCCCGCT CCTTCAAGGA GGAGTTGCTC 
TGCGCCGTCT GCTACGACCC CTTCCGCGAC GCAGTCACTC TGCGCTGCGG CCACAACTTC 
TGCCGCGGGT GCGTGAGCCG CTGCTGGGAG GTGCAGGTGT CGCCCACCTG CCCAGTGTGC 
AAAGACCGCG CGTCACCCGC CGACCTGCGC ACCAACCACA CCCTCAACAA CCTGGTGGAG 
AAGCTGCTGC GCGAGGAGGC CGAGGGCGCG CGCTGGACCA GCTACCGCTT CTCGCGTGTC 
TGCCGCCTGC ACCGCGGACA GCTCAGCCTC TTCTGCCTCG AGGACAAGGA GCTGCTGTGC 
TGCTCCTGCC AGGCCGACCC CCGACACCAG GGGCACCGCG TGCAGCCGGT GAAGGACACT 
GCCCACGACT TTCGGGCCAA GTGCAGGAAC ATGGAGCATG CACTGCGGGA GAAGGCCAAG 
GCCTTCTGGG CCATGCGGCG CTCCTATGAG GCCATCGCCA AGCACAATCA GGTGGAGGCT 
GCATGGCTGG AAGGCCGGAT CCGGCAGGAG TTTGATAAGC TTCGCGAGTT CTTGAGAGTG 
GAGGAGCAGG CCATTCTGGA TGCCATGGCC GAGGAGACAA GGCAGAAGCA ACTTCTGGCC 
GACGAGAAGA TGAAGCAGCT CACAGAGGAG ACGGAGGTGC TGGCACATGA GATCGAGCGG 
CTGCAGATGG AGATGAAGGA GGACGACGTT TCTTTTCTCA TGACTCTTCT GCACCATGGA 
GCCAGAGCCA GTCCAGCCCG GCATGCTTAT CGATGTCTGC AAGTACCTGG GCTCCCTGCA 
GTACCGCGTC TGGAAGAAGA TGCTTGCATC TGTGGAATCT GTACCCTTCA GCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM35 ( NM_001362813.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001349742.1 Homo sapiens tripartite motif containing 35 (TRIM35), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu42024
Clone ID Related Accession (Same CDS sequence)	XM_005273452.3 , NM_001362813.1 , NM_001362813.2
Accession Version	NM_001362813.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 35 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF492463.1, AC124649.6 and BU622420.1. On May 31, 2019 this sequence version replaced NM_001362813.1. Transcript Variant: This variant (3) lacks an alternate exon in the central coding region, resulting in a frameshift compared to variant 1. The encoded isoform (3) is shorter and has a distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.16580.1, SRR1803612.135832.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2161674 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCGGA GTCCCGACGT GTCCCCCGGG CCTTCCCGCT CCTTCAAGGA GGAGTTGCTC 
TGCGCCGTCT GCTACGACCC CTTCCGCGAC GCAGTCACTC TGCGCTGCGG CCACAACTTC 
TGCCGCGGGT GCGTGAGCCG CTGCTGGGAG GTGCAGGTGT CGCCCACCTG CCCAGTGTGC 
AAAGACCGCG CGTCACCCGC CGACCTGCGC ACCAACCACA CCCTCAACAA CCTGGTGGAG 
AAGCTGCTGC GCGAGGAGGC CGAGGGCGCG CGCTGGACCA GCTACCGCTT CTCGCGTGTC 
TGCCGCCTGC ACCGCGGACA GCTCAGCCTC TTCTGCCTCG AGGACAAGGA GCTGCTGTGC 
TGCTCCTGCC AGGCCGACCC CCGACACCAG GGGCACCGCG TGCAGCCGGT GAAGGACACT 
GCCCACGACT TTCGGGCCAA GTGCAGGAAC ATGGAGCATG CACTGCGGGA GAAGGCCAAG 
GCCTTCTGGG CCATGCGGCG CTCCTATGAG GCCATCGCCA AGCACAATCA GGTGGAGGCT 
GCATGGCTGG AAGGCCGGAT CCGGCAGGAG TTTGATAAGC TTCGCGAGTT CTTGAGAGTG 
GAGGAGCAGG CCATTCTGGA TGCCATGGCC GAGGAGACAA GGCAGAAGCA ACTTCTGGCC 
GACGAGAAGA TGAAGCAGCT CACAGAGGAG ACGGAGGTGC TGGCACATGA GATCGAGCGG 
CTGCAGATGG AGATGAAGGA GGACGACGTT TCTTTTCTCA TGACTCTTCT GCACCATGGA 
GCCAGAGCCA GTCCAGCCCG GCATGCTTAT CGATGTCTGC AAGTACCTGG GCTCCCTGCA 
GTACCGCGTC TGGAAGAAGA TGCTTGCATC TGTGGAATCT GTACCCTTCA GCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349742.1
CDS	38..934
Translation	MERSPDVSPGPSRSFKEELLCAVCYDPFRDAVTLRCGHNFCRGCVSRCWEVQVSPTCPVCKDRASPADLRTNHTLNNLVEKLLREEAEGARWTSYRFSRVCRLHRGQLSLFCLEDKELLCCSCQADPRHQGHRVQPVKDTAHDFRAKCRNMEHALREKAKAFWAMRRSYEAIAKHNQVEAAWLEGRIRQEFDKLREFLRVEEQAILDAMAEETRQKQLLADEKMKQLTEETEVLAHEIERLQMEMKEDDVSFLMTLLHHGARASPARHAYRCLQVPGLPAVPRLEEDACICGICTLQL

Target ORF information:

RefSeq Version	NM_001362813.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 35 (TRIM35), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362813.2

ORF Insert Sequence:

ATGGAGCGGA GTCCCGACGT GTCCCCCGGG CCTTCCCGCT CCTTCAAGGA GGAGTTGCTC 
TGCGCCGTCT GCTACGACCC CTTCCGCGAC GCAGTCACTC TGCGCTGCGG CCACAACTTC 
TGCCGCGGGT GCGTGAGCCG CTGCTGGGAG GTGCAGGTGT CGCCCACCTG CCCAGTGTGC 
AAAGACCGCG CGTCACCCGC CGACCTGCGC ACCAACCACA CCCTCAACAA CCTGGTGGAG 
AAGCTGCTGC GCGAGGAGGC CGAGGGCGCG CGCTGGACCA GCTACCGCTT CTCGCGTGTC 
TGCCGCCTGC ACCGCGGACA GCTCAGCCTC TTCTGCCTCG AGGACAAGGA GCTGCTGTGC 
TGCTCCTGCC AGGCCGACCC CCGACACCAG GGGCACCGCG TGCAGCCGGT GAAGGACACT 
GCCCACGACT TTCGGGCCAA GTGCAGGAAC ATGGAGCATG CACTGCGGGA GAAGGCCAAG 
GCCTTCTGGG CCATGCGGCG CTCCTATGAG GCCATCGCCA AGCACAATCA GGTGGAGGCT 
GCATGGCTGG AAGGCCGGAT CCGGCAGGAG TTTGATAAGC TTCGCGAGTT CTTGAGAGTG 
GAGGAGCAGG CCATTCTGGA TGCCATGGCC GAGGAGACAA GGCAGAAGCA ACTTCTGGCC 
GACGAGAAGA TGAAGCAGCT CACAGAGGAG ACGGAGGTGC TGGCACATGA GATCGAGCGG 
CTGCAGATGG AGATGAAGGA GGACGACGTT TCTTTTCTCA TGACTCTTCT GCACCATGGA 
GCCAGAGCCA GTCCAGCCCG GCATGCTTAT CGATGTCTGC AAGTACCTGG GCTCCCTGCA 
GTACCGCGTC TGGAAGAAGA TGCTTGCATC TGTGGAATCT GTACCCTTCA GCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM35 ( NM_001304495.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001291424.1 Homo sapiens tripartite motif containing 35 (TRIM35), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu42025
Clone ID Related Accession (Same CDS sequence)	NM_001304495.2 , NM_001304495.1
Accession Version	NM_001304495.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565020800000
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 35 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124649.6, AK308236.1 and BU622420.1. On May 31, 2019 this sequence version replaced NM_001304495.1. Transcript Variant: This variant (2) lacks two exons in the central coding region one of which results in a frameshift compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.12648.1, AK308236.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCGGA GTCCCGACGT GTCCCCCGGG CCTTCCCGCT CCTTCAAGGA GGAGTTGCTC 
TGCGCCGTCT GCTACGACCC CTTCCGCGAC GCAGTCACTC TGCGCTGCGG CCACAACTTC 
TGCCGCGGGT GCGTGAGCCG CTGCTGGGAG GTGCAGGTGT CGCCCACCTG CCCAGTGTGC 
AAAGACCGCG CGTCACCCGC CGACCTGCGC ACCAACCACA CCCTCAACAA CCTGGTGGAG 
AAGCTGCTGC GCGAGGAGGC CGAGGGCGCG CGCTGGACCA GCTACCGCTT CTCGCGTGTC 
TGCCGCCTGC ACCGCGGACA GCTCAGCCTC TTCTGCCTCG AGGACAAGGA GCTGCTGTGC 
TGCTCCTGCC AGGCCGACCC CCGACACCAG GGGCACCGCG TGCAGCCGGT GAAGGACACT 
GCCCACGACT TTCGGGTGGA GGCTGCATGG CTGGAAGGCC GGATCCGGCA GGAGTTTGAT 
AAGCTTCGCG AGTTCTTGAG AGTGGAGGAG CAGGCCATTC TGGATGCCAT GGCCGAGGAG 
ACAAGGCAGA AGCAACTTCT GGCCGACGAG AAGATGAAGC AGCTCACAGA GGAGACGGAG 
GTGCTGGCAC ATGAGATCGA GCGGCTGCAG ATGGAGATGA AGGAGGACGA CGTTTCTTTT 
CTCATGACTC TTCTGCACCA TGGAGCCAGA GCCAGTCCAG CCCGGCATGC TTATCGATGT 
CTGCAAGTAC CTGGGCTCCC TGCAGTACCG CGTCTGGAAG AAGATGCTTG CATCTGTGGA 
ATCTGTACCC TTCAGCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001291424.1
CDS	38..838
Translation	MERSPDVSPGPSRSFKEELLCAVCYDPFRDAVTLRCGHNFCRGCVSRCWEVQVSPTCPVCKDRASPADLRTNHTLNNLVEKLLREEAEGARWTSYRFSRVCRLHRGQLSLFCLEDKELLCCSCQADPRHQGHRVQPVKDTAHDFRVEAAWLEGRIRQEFDKLREFLRVEEQAILDAMAEETRQKQLLADEKMKQLTEETEVLAHEIERLQMEMKEDDVSFLMTLLHHGARASPARHAYRCLQVPGLPAVPRLEEDACICGICTLQL

Target ORF information:

RefSeq Version	NM_001304495.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 35 (TRIM35), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001304495.2

ORF Insert Sequence:

ATGGAGCGGA GTCCCGACGT GTCCCCCGGG CCTTCCCGCT CCTTCAAGGA GGAGTTGCTC 
TGCGCCGTCT GCTACGACCC CTTCCGCGAC GCAGTCACTC TGCGCTGCGG CCACAACTTC 
TGCCGCGGGT GCGTGAGCCG CTGCTGGGAG GTGCAGGTGT CGCCCACCTG CCCAGTGTGC 
AAAGACCGCG CGTCACCCGC CGACCTGCGC ACCAACCACA CCCTCAACAA CCTGGTGGAG 
AAGCTGCTGC GCGAGGAGGC CGAGGGCGCG CGCTGGACCA GCTACCGCTT CTCGCGTGTC 
TGCCGCCTGC ACCGCGGACA GCTCAGCCTC TTCTGCCTCG AGGACAAGGA GCTGCTGTGC 
TGCTCCTGCC AGGCCGACCC CCGACACCAG GGGCACCGCG TGCAGCCGGT GAAGGACACT 
GCCCACGACT TTCGGGTGGA GGCTGCATGG CTGGAAGGCC GGATCCGGCA GGAGTTTGAT 
AAGCTTCGCG AGTTCTTGAG AGTGGAGGAG CAGGCCATTC TGGATGCCAT GGCCGAGGAG 
ACAAGGCAGA AGCAACTTCT GGCCGACGAG AAGATGAAGC AGCTCACAGA GGAGACGGAG 
GTGCTGGCAC ATGAGATCGA GCGGCTGCAG ATGGAGATGA AGGAGGACGA CGTTTCTTTT 
CTCATGACTC TTCTGCACCA TGGAGCCAGA GCCAGTCCAG CCCGGCATGC TTATCGATGT 
CTGCAAGTAC CTGGGCTCCC TGCAGTACCG CGTCTGGAAG AAGATGCTTG CATCTGTGGA 
ATCTGTACCC TTCAGCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM35 ( NM_001304495.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001291424.1 Homo sapiens tripartite motif containing 35 (TRIM35), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu42025
Clone ID Related Accession (Same CDS sequence)	NM_001304495.2 , NM_001304495.1
Accession Version	NM_001304495.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1530288000000
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 35 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124649.6, AK308236.1 and BU622420.1. On Jan 29, 2015 this sequence version replaced XM_005273453.1. Transcript Variant: This variant (2) lacks two exons in the central coding region one of which results in a frameshift compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.12648.1, AK308236.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCGGA GTCCCGACGT GTCCCCCGGG CCTTCCCGCT CCTTCAAGGA GGAGTTGCTC 
TGCGCCGTCT GCTACGACCC CTTCCGCGAC GCAGTCACTC TGCGCTGCGG CCACAACTTC 
TGCCGCGGGT GCGTGAGCCG CTGCTGGGAG GTGCAGGTGT CGCCCACCTG CCCAGTGTGC 
AAAGACCGCG CGTCACCCGC CGACCTGCGC ACCAACCACA CCCTCAACAA CCTGGTGGAG 
AAGCTGCTGC GCGAGGAGGC CGAGGGCGCG CGCTGGACCA GCTACCGCTT CTCGCGTGTC 
TGCCGCCTGC ACCGCGGACA GCTCAGCCTC TTCTGCCTCG AGGACAAGGA GCTGCTGTGC 
TGCTCCTGCC AGGCCGACCC CCGACACCAG GGGCACCGCG TGCAGCCGGT GAAGGACACT 
GCCCACGACT TTCGGGTGGA GGCTGCATGG CTGGAAGGCC GGATCCGGCA GGAGTTTGAT 
AAGCTTCGCG AGTTCTTGAG AGTGGAGGAG CAGGCCATTC TGGATGCCAT GGCCGAGGAG 
ACAAGGCAGA AGCAACTTCT GGCCGACGAG AAGATGAAGC AGCTCACAGA GGAGACGGAG 
GTGCTGGCAC ATGAGATCGA GCGGCTGCAG ATGGAGATGA AGGAGGACGA CGTTTCTTTT 
CTCATGACTC TTCTGCACCA TGGAGCCAGA GCCAGTCCAG CCCGGCATGC TTATCGATGT 
CTGCAAGTAC CTGGGCTCCC TGCAGTACCG CGTCTGGAAG AAGATGCTTG CATCTGTGGA 
ATCTGTACCC TTCAGCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001291424.1
CDS	85..885
Translation	MERSPDVSPGPSRSFKEELLCAVCYDPFRDAVTLRCGHNFCRGCVSRCWEVQVSPTCPVCKDRASPADLRTNHTLNNLVEKLLREEAEGARWTSYRFSRVCRLHRGQLSLFCLEDKELLCCSCQADPRHQGHRVQPVKDTAHDFRVEAAWLEGRIRQEFDKLREFLRVEEQAILDAMAEETRQKQLLADEKMKQLTEETEVLAHEIERLQMEMKEDDVSFLMTLLHHGARASPARHAYRCLQVPGLPAVPRLEEDACICGICTLQL

Target ORF information:

RefSeq Version	NM_001304495.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 35 (TRIM35), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001304495.1

ORF Insert Sequence:

ATGGAGCGGA GTCCCGACGT GTCCCCCGGG CCTTCCCGCT CCTTCAAGGA GGAGTTGCTC 
TGCGCCGTCT GCTACGACCC CTTCCGCGAC GCAGTCACTC TGCGCTGCGG CCACAACTTC 
TGCCGCGGGT GCGTGAGCCG CTGCTGGGAG GTGCAGGTGT CGCCCACCTG CCCAGTGTGC 
AAAGACCGCG CGTCACCCGC CGACCTGCGC ACCAACCACA CCCTCAACAA CCTGGTGGAG 
AAGCTGCTGC GCGAGGAGGC CGAGGGCGCG CGCTGGACCA GCTACCGCTT CTCGCGTGTC 
TGCCGCCTGC ACCGCGGACA GCTCAGCCTC TTCTGCCTCG AGGACAAGGA GCTGCTGTGC 
TGCTCCTGCC AGGCCGACCC CCGACACCAG GGGCACCGCG TGCAGCCGGT GAAGGACACT 
GCCCACGACT TTCGGGTGGA GGCTGCATGG CTGGAAGGCC GGATCCGGCA GGAGTTTGAT 
AAGCTTCGCG AGTTCTTGAG AGTGGAGGAG CAGGCCATTC TGGATGCCAT GGCCGAGGAG 
ACAAGGCAGA AGCAACTTCT GGCCGACGAG AAGATGAAGC AGCTCACAGA GGAGACGGAG 
GTGCTGGCAC ATGAGATCGA GCGGCTGCAG ATGGAGATGA AGGAGGACGA CGTTTCTTTT 
CTCATGACTC TTCTGCACCA TGGAGCCAGA GCCAGTCCAG CCCGGCATGC TTATCGATGT 
CTGCAAGTAC CTGGGCTCCC TGCAGTACCG CGTCTGGAAG AAGATGCTTG CATCTGTGGA 
ATCTGTACCC TTCAGCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM35 ( XM_005273452.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005273509.1 Homo sapiens tripartite motif containing 35 (TRIM35), transcript variant X2, mRNA.

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CloneID	OHu42024
Clone ID Related Accession (Same CDS sequence)	XM_005273452.3 , NM_001362813.1 , NM_001362813.2
Accession Version	XM_005273452.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 35 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005273452.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGA GTCCCGACGT GTCCCCCGGG CCTTCCCGCT CCTTCAAGGA GGAGTTGCTC 
TGCGCCGTCT GCTACGACCC CTTCCGCGAC GCAGTCACTC TGCGCTGCGG CCACAACTTC 
TGCCGCGGGT GCGTGAGCCG CTGCTGGGAG GTGCAGGTGT CGCCCACCTG CCCAGTGTGC 
AAAGACCGCG CGTCACCCGC CGACCTGCGC ACCAACCACA CCCTCAACAA CCTGGTGGAG 
AAGCTGCTGC GCGAGGAGGC CGAGGGCGCG CGCTGGACCA GCTACCGCTT CTCGCGTGTC 
TGCCGCCTGC ACCGCGGACA GCTCAGCCTC TTCTGCCTCG AGGACAAGGA GCTGCTGTGC 
TGCTCCTGCC AGGCCGACCC CCGACACCAG GGGCACCGCG TGCAGCCGGT GAAGGACACT 
GCCCACGACT TTCGGGCCAA GTGCAGGAAC ATGGAGCATG CACTGCGGGA GAAGGCCAAG 
GCCTTCTGGG CCATGCGGCG CTCCTATGAG GCCATCGCCA AGCACAATCA GGTGGAGGCT 
GCATGGCTGG AAGGCCGGAT CCGGCAGGAG TTTGATAAGC TTCGCGAGTT CTTGAGAGTG 
GAGGAGCAGG CCATTCTGGA TGCCATGGCC GAGGAGACAA GGCAGAAGCA ACTTCTGGCC 
GACGAGAAGA TGAAGCAGCT CACAGAGGAG ACGGAGGTGC TGGCACATGA GATCGAGCGG 
CTGCAGATGG AGATGAAGGA GGACGACGTT TCTTTTCTCA TGACTCTTCT GCACCATGGA 
GCCAGAGCCA GTCCAGCCCG GCATGCTTAT CGATGTCTGC AAGTACCTGG GCTCCCTGCA 
GTACCGCGTC TGGAAGAAGA TGCTTGCATC TGTGGAATCT GTACCCTTCA GCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005273509.1
CDS	80..976
Translation	MERSPDVSPGPSRSFKEELLCAVCYDPFRDAVTLRCGHNFCRGCVSRCWEVQVSPTCPVCKDRASPADLRTNHTLNNLVEKLLREEAEGARWTSYRFSRVCRLHRGQLSLFCLEDKELLCCSCQADPRHQGHRVQPVKDTAHDFRAKCRNMEHALREKAKAFWAMRRSYEAIAKHNQVEAAWLEGRIRQEFDKLREFLRVEEQAILDAMAEETRQKQLLADEKMKQLTEETEVLAHEIERLQMEMKEDDVSFLMTLLHHGARASPARHAYRCLQVPGLPAVPRLEEDACICGICTLQL

Target ORF information:

RefSeq Version	XM_005273452.3
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 35 (TRIM35), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005273452.3

ORF Insert Sequence:

ATGGAGCGGA GTCCCGACGT GTCCCCCGGG CCTTCCCGCT CCTTCAAGGA GGAGTTGCTC 
TGCGCCGTCT GCTACGACCC CTTCCGCGAC GCAGTCACTC TGCGCTGCGG CCACAACTTC 
TGCCGCGGGT GCGTGAGCCG CTGCTGGGAG GTGCAGGTGT CGCCCACCTG CCCAGTGTGC 
AAAGACCGCG CGTCACCCGC CGACCTGCGC ACCAACCACA CCCTCAACAA CCTGGTGGAG 
AAGCTGCTGC GCGAGGAGGC CGAGGGCGCG CGCTGGACCA GCTACCGCTT CTCGCGTGTC 
TGCCGCCTGC ACCGCGGACA GCTCAGCCTC TTCTGCCTCG AGGACAAGGA GCTGCTGTGC 
TGCTCCTGCC AGGCCGACCC CCGACACCAG GGGCACCGCG TGCAGCCGGT GAAGGACACT 
GCCCACGACT TTCGGGCCAA GTGCAGGAAC ATGGAGCATG CACTGCGGGA GAAGGCCAAG 
GCCTTCTGGG CCATGCGGCG CTCCTATGAG GCCATCGCCA AGCACAATCA GGTGGAGGCT 
GCATGGCTGG AAGGCCGGAT CCGGCAGGAG TTTGATAAGC TTCGCGAGTT CTTGAGAGTG 
GAGGAGCAGG CCATTCTGGA TGCCATGGCC GAGGAGACAA GGCAGAAGCA ACTTCTGGCC 
GACGAGAAGA TGAAGCAGCT CACAGAGGAG ACGGAGGTGC TGGCACATGA GATCGAGCGG 
CTGCAGATGG AGATGAAGGA GGACGACGTT TCTTTTCTCA TGACTCTTCT GCACCATGGA 
GCCAGAGCCA GTCCAGCCCG GCATGCTTAT CGATGTCTGC AAGTACCTGG GCTCCCTGCA 
GTACCGCGTC TGGAAGAAGA TGCTTGCATC TGTGGAATCT GTACCCTTCA GCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM35 ( XM_011544453.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542755.1 Homo sapiens tripartite motif containing 35 (TRIM35), transcript variant X1, mRNA.

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CloneID	OHu65417
Clone ID Related Accession (Same CDS sequence)	XM_011544453.1
Accession Version	XM_011544453.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 35 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCATG CACTGCGGGA GAAGGCCAAG GCCTTCTGGG CCATGCGGCG CTCCTATGAG 
GCCATCGCCA AGCACAATCA GGTGGAGGCT GCATGGCTGG AAGGCCGGAT CCGGCAGGAG 
TTTGATAAGC TTCGCGAGTT CTTGAGAGTG GAGGAGCAGG CCATTCTGGA TGCCATGGCC 
GAGGAGACAA GGCAGAAGCA ACTTCTGGCC GACGAGAAGA TGAAGCAGCT CACAGAGGAG 
ACGGAGGTGC TGGCACATGA GATCGAGCGG CTGCAGATGG AGATGAAGGA GGACGACGTT 
TCTTTTCTCA TGAAACACAA GAGCCGAAAA CGCCGACTCT TCTGCACCAT GGAGCCAGAG 
CCAGTCCAGC CCGGCATGCT TATCGATGTC TGCAAGTACC TGGGCTCCCT GCAGTACCGC 
GTCTGGAAGA AGATGCTTGC ATCTGTGGAA TCTGTACCCT TCAGCTTTGA CCCCAACACC 
GCAGCTGGCT GGCTCTCCGT GTCTGACGAC CTCACCAGCG TCACCAACCA TGGCTACCGC 
GTGCAGGTGG AGAACCCGGA ACGCTTCTCC TCGGCGCCCT GCCTGCTGGG CTCCCGTGTC 
TTCTCACAGG GCTCGCACGC CTGGGAGGTG GCCCTTGGGG GGCTGCAGAG CTGGAGGGTG 
GGCGTGGTAC GTGTGCGCCA GGACTCGGGC GCTGAGGGCC ACTCACACAG CTGCTACCAC 
GACACACGCT CGGGCTTCTG GTATGTCTGC CGCACGCAGG GCGTGGAGGG GGACCACTGC 
GTGACCTCGG ACCCAGCCAC GTCGCCCCTG GTCCTGGCCA TCCCACGCCG CCTGCGTGTG 
GAGCTGGAGT GTGAGGAGGG CGAGCTGTCT TTCTATGACG CGGAGCGCCA CTGCCACCTG 
TACACCTTCC ACGCCCGCTT TGGGGAGGTT CGCCCCTACT TCTACCTGGG GGGTGCACGG 
GGCGCCGGGC CTCCAGAGCC TTTGCGCATC TGCCCCTTGC ACATCAGTGT CAAGGAAGAA 
CTGGATGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542755.1
CDS	563..1594
Translation	MEHALREKAKAFWAMRRSYEAIAKHNQVEAAWLEGRIRQEFDKLREFLRVEEQAILDAMAEETRQKQLLADEKMKQLTEETEVLAHEIERLQMEMKEDDVSFLMKHKSRKRRLFCTMEPEPVQPGMLIDVCKYLGSLQYRVWKKMLASVESVPFSFDPNTAAGWLSVSDDLTSVTNHGYRVQVENPERFSSAPCLLGSRVFSQGSHAWEVALGGLQSWRVGVVRVRQDSGAEGHSHSCYHDTRSGFWYVCRTQGVEGDHCVTSDPATSPLVLAIPRRLRVELECEEGELSFYDAERHCHLYTFHARFGEVRPYFYLGGARGAGPPEPLRICPLHISVKEELDG

Target ORF information:

RefSeq Version	XM_011544453.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 35 (TRIM35), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544453.1

ORF Insert Sequence:

ATGGAGCATG CACTGCGGGA GAAGGCCAAG GCCTTCTGGG CCATGCGGCG CTCCTATGAG 
GCCATCGCCA AGCACAATCA GGTGGAGGCT GCATGGCTGG AAGGCCGGAT CCGGCAGGAG 
TTTGATAAGC TTCGCGAGTT CTTGAGAGTG GAGGAGCAGG CCATTCTGGA TGCCATGGCC 
GAGGAGACAA GGCAGAAGCA ACTTCTGGCC GACGAGAAGA TGAAGCAGCT CACAGAGGAG 
ACGGAGGTGC TGGCACATGA GATCGAGCGG CTGCAGATGG AGATGAAGGA GGACGACGTT 
TCTTTTCTCA TGAAACACAA GAGCCGAAAA CGCCGACTCT TCTGCACCAT GGAGCCAGAG 
CCAGTCCAGC CCGGCATGCT TATCGATGTC TGCAAGTACC TGGGCTCCCT GCAGTACCGC 
GTCTGGAAGA AGATGCTTGC ATCTGTGGAA TCTGTACCCT TCAGCTTTGA CCCCAACACC 
GCAGCTGGCT GGCTCTCCGT GTCTGACGAC CTCACCAGCG TCACCAACCA TGGCTACCGC 
GTGCAGGTGG AGAACCCGGA ACGCTTCTCC TCGGCGCCCT GCCTGCTGGG CTCCCGTGTC 
TTCTCACAGG GCTCGCACGC CTGGGAGGTG GCCCTTGGGG GGCTGCAGAG CTGGAGGGTG 
GGCGTGGTAC GTGTGCGCCA GGACTCGGGC GCTGAGGGCC ACTCACACAG CTGCTACCAC 
GACACACGCT CGGGCTTCTG GTATGTCTGC CGCACGCAGG GCGTGGAGGG GGACCACTGC 
GTGACCTCGG ACCCAGCCAC GTCGCCCCTG GTCCTGGCCA TCCCACGCCG CCTGCGTGTG 
GAGCTGGAGT GTGAGGAGGG CGAGCTGTCT TTCTATGACG CGGAGCGCCA CTGCCACCTG 
TACACCTTCC ACGCCCGCTT TGGGGAGGTT CGCCCCTACT TCTACCTGGG GGGTGCACGG 
GGCGCCGGGC CTCCAGAGCC TTTGCGCATC TGCCCCTTGC ACATCAGTGT CAAGGAAGAA 
CTGGATGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM35 ( NM_171982.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_741983.2 Homo sapiens tripartite motif containing 35 (TRIM35), transcript variant 1, mRNA.

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CloneID	OHu07109
Clone ID Related Accession (Same CDS sequence)	NM_171982.5 , NM_171982.4
Accession Version	NM_171982.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565020800000
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 35 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF492463.1, AC124649.6 and BU622420.1. On Nov 23, 2018 this sequence version replaced NM_171982.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.161349.1, AB029021.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000305364.9/ ENSP00000301924.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCGGA GTCCCGACGT GTCCCCCGGG CCTTCCCGCT CCTTCAAGGA GGAGTTGCTC 
TGCGCCGTCT GCTACGACCC CTTCCGCGAC GCAGTCACTC TGCGCTGCGG CCACAACTTC 
TGCCGCGGGT GCGTGAGCCG CTGCTGGGAG GTGCAGGTGT CGCCCACCTG CCCAGTGTGC 
AAAGACCGCG CGTCACCCGC CGACCTGCGC ACCAACCACA CCCTCAACAA CCTGGTGGAG 
AAGCTGCTGC GCGAGGAGGC CGAGGGCGCG CGCTGGACCA GCTACCGCTT CTCGCGTGTC 
TGCCGCCTGC ACCGCGGACA GCTCAGCCTC TTCTGCCTCG AGGACAAGGA GCTGCTGTGC 
TGCTCCTGCC AGGCCGACCC CCGACACCAG GGGCACCGCG TGCAGCCGGT GAAGGACACT 
GCCCACGACT TTCGGGCCAA GTGCAGGAAC ATGGAGCATG CACTGCGGGA GAAGGCCAAG 
GCCTTCTGGG CCATGCGGCG CTCCTATGAG GCCATCGCCA AGCACAATCA GGTGGAGGCT 
GCATGGCTGG AAGGCCGGAT CCGGCAGGAG TTTGATAAGC TTCGCGAGTT CTTGAGAGTG 
GAGGAGCAGG CCATTCTGGA TGCCATGGCC GAGGAGACAA GGCAGAAGCA ACTTCTGGCC 
GACGAGAAGA TGAAGCAGCT CACAGAGGAG ACGGAGGTGC TGGCACATGA GATCGAGCGG 
CTGCAGATGG AGATGAAGGA GGACGACGTT TCTTTTCTCA TGAAACACAA GAGCCGAAAA 
CGCCGACTCT TCTGCACCAT GGAGCCAGAG CCAGTCCAGC CCGGCATGCT TATCGATGTC 
TGCAAGTACC TGGGCTCCCT GCAGTACCGC GTCTGGAAGA AGATGCTTGC ATCTGTGGAA 
TCTGTACCCT TCAGCTTTGA CCCCAACACC GCAGCTGGCT GGCTCTCCGT GTCTGACGAC 
CTCACCAGCG TCACCAACCA TGGCTACCGC GTGCAGGTGG AGAACCCGGA ACGCTTCTCC 
TCGGCGCCCT GCCTGCTGGG CTCCCGTGTC TTCTCACAGG GCTCGCACGC CTGGGAGGTG 
GCCCTTGGGG GGCTGCAGAG CTGGAGGGTG GGCGTGGTAC GTGTGCGCCA GGACTCGGGC 
GCTGAGGGCC ACTCACACAG CTGCTACCAC GACACACGCT CGGGCTTCTG GTATGTCTGC 
CGCACGCAGG GCGTGGAGGG GGACCACTGC GTGACCTCGG ACCCAGCCAC GTCGCCCCTG 
GTCCTGGCCA TCCCACGCCG CCTGCGTGTG GAGCTGGAGT GTGAGGAGGG CGAGCTGTCT 
TTCTATGACG CGGAGCGCCA CTGCCACCTG TACACCTTCC ACGCCCGCTT TGGGGAGGTT 
CGCCCCTACT TCTACCTGGG GGGTGCACGG GGCGCCGGGC CTCCAGAGCC TTTGCGCATC 
TGCCCCTTGC ACATCAGTGT CAAGGAAGAA CTGGATGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_741983.2
CDS	38..1519
Translation	MERSPDVSPGPSRSFKEELLCAVCYDPFRDAVTLRCGHNFCRGCVSRCWEVQVSPTCPVCKDRASPADLRTNHTLNNLVEKLLREEAEGARWTSYRFSRVCRLHRGQLSLFCLEDKELLCCSCQADPRHQGHRVQPVKDTAHDFRAKCRNMEHALREKAKAFWAMRRSYEAIAKHNQVEAAWLEGRIRQEFDKLREFLRVEEQAILDAMAEETRQKQLLADEKMKQLTEETEVLAHEIERLQMEMKEDDVSFLMKHKSRKRRLFCTMEPEPVQPGMLIDVCKYLGSLQYRVWKKMLASVESVPFSFDPNTAAGWLSVSDDLTSVTNHGYRVQVENPERFSSAPCLLGSRVFSQGSHAWEVALGGLQSWRVGVVRVRQDSGAEGHSHSCYHDTRSGFWYVCRTQGVEGDHCVTSDPATSPLVLAIPRRLRVELECEEGELSFYDAERHCHLYTFHARFGEVRPYFYLGGARGAGPPEPLRICPLHISVKEELDG

Target ORF information:

RefSeq Version	NM_171982.5
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 35 (TRIM35), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_171982.5

ORF Insert Sequence:

ATGGAGCGGA GTCCCGACGT GTCCCCCGGG CCTTCCCGCT CCTTCAAGGA GGAGTTGCTC 
TGCGCCGTCT GCTACGACCC CTTCCGCGAC GCAGTCACTC TGCGCTGCGG CCACAACTTC 
TGCCGCGGGT GCGTGAGCCG CTGCTGGGAG GTGCAGGTGT CGCCCACCTG CCCAGTGTGC 
AAAGACCGCG CGTCACCCGC CGACCTGCGC ACCAACCACA CCCTCAACAA CCTGGTGGAG 
AAGCTGCTGC GCGAGGAGGC CGAGGGCGCG CGCTGGACCA GCTACCGCTT CTCGCGTGTC 
TGCCGCCTGC ACCGCGGACA GCTCAGCCTC TTCTGCCTCG AGGACAAGGA GCTGCTGTGC 
TGCTCCTGCC AGGCCGACCC CCGACACCAG GGGCACCGCG TGCAGCCGGT GAAGGACACT 
GCCCACGACT TTCGGGCCAA GTGCAGGAAC ATGGAGCATG CACTGCGGGA GAAGGCCAAG 
GCCTTCTGGG CCATGCGGCG CTCCTATGAG GCCATCGCCA AGCACAATCA GGTGGAGGCT 
GCATGGCTGG AAGGCCGGAT CCGGCAGGAG TTTGATAAGC TTCGCGAGTT CTTGAGAGTG 
GAGGAGCAGG CCATTCTGGA TGCCATGGCC GAGGAGACAA GGCAGAAGCA ACTTCTGGCC 
GACGAGAAGA TGAAGCAGCT CACAGAGGAG ACGGAGGTGC TGGCACATGA GATCGAGCGG 
CTGCAGATGG AGATGAAGGA GGACGACGTT TCTTTTCTCA TGAAACACAA GAGCCGAAAA 
CGCCGACTCT TCTGCACCAT GGAGCCAGAG CCAGTCCAGC CCGGCATGCT TATCGATGTC 
TGCAAGTACC TGGGCTCCCT GCAGTACCGC GTCTGGAAGA AGATGCTTGC ATCTGTGGAA 
TCTGTACCCT TCAGCTTTGA CCCCAACACC GCAGCTGGCT GGCTCTCCGT GTCTGACGAC 
CTCACCAGCG TCACCAACCA TGGCTACCGC GTGCAGGTGG AGAACCCGGA ACGCTTCTCC 
TCGGCGCCCT GCCTGCTGGG CTCCCGTGTC TTCTCACAGG GCTCGCACGC CTGGGAGGTG 
GCCCTTGGGG GGCTGCAGAG CTGGAGGGTG GGCGTGGTAC GTGTGCGCCA GGACTCGGGC 
GCTGAGGGCC ACTCACACAG CTGCTACCAC GACACACGCT CGGGCTTCTG GTATGTCTGC 
CGCACGCAGG GCGTGGAGGG GGACCACTGC GTGACCTCGG ACCCAGCCAC GTCGCCCCTG 
GTCCTGGCCA TCCCACGCCG CCTGCGTGTG GAGCTGGAGT GTGAGGAGGG CGAGCTGTCT 
TTCTATGACG CGGAGCGCCA CTGCCACCTG TACACCTTCC ACGCCCGCTT TGGGGAGGTT 
CGCCCCTACT TCTACCTGGG GGGTGCACGG GGCGCCGGGC CTCCAGAGCC TTTGCGCATC 
TGCCCCTTGC ACATCAGTGT CAAGGAAGAA CTGGATGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM35 ( NM_171982.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_741983.2 Homo sapiens tripartite motif containing 35 (TRIM35), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu07109
Clone ID Related Accession (Same CDS sequence)	NM_171982.5 , NM_171982.4
Accession Version	NM_171982.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 35 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124649.6, AF492463.1 and BU622420.1. On Jan 29, 2015 this sequence version replaced NM_171982.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AF492463.1, SRR1660805.161349.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCGGA GTCCCGACGT GTCCCCCGGG CCTTCCCGCT CCTTCAAGGA GGAGTTGCTC 
TGCGCCGTCT GCTACGACCC CTTCCGCGAC GCAGTCACTC TGCGCTGCGG CCACAACTTC 
TGCCGCGGGT GCGTGAGCCG CTGCTGGGAG GTGCAGGTGT CGCCCACCTG CCCAGTGTGC 
AAAGACCGCG CGTCACCCGC CGACCTGCGC ACCAACCACA CCCTCAACAA CCTGGTGGAG 
AAGCTGCTGC GCGAGGAGGC CGAGGGCGCG CGCTGGACCA GCTACCGCTT CTCGCGTGTC 
TGCCGCCTGC ACCGCGGACA GCTCAGCCTC TTCTGCCTCG AGGACAAGGA GCTGCTGTGC 
TGCTCCTGCC AGGCCGACCC CCGACACCAG GGGCACCGCG TGCAGCCGGT GAAGGACACT 
GCCCACGACT TTCGGGCCAA GTGCAGGAAC ATGGAGCATG CACTGCGGGA GAAGGCCAAG 
GCCTTCTGGG CCATGCGGCG CTCCTATGAG GCCATCGCCA AGCACAATCA GGTGGAGGCT 
GCATGGCTGG AAGGCCGGAT CCGGCAGGAG TTTGATAAGC TTCGCGAGTT CTTGAGAGTG 
GAGGAGCAGG CCATTCTGGA TGCCATGGCC GAGGAGACAA GGCAGAAGCA ACTTCTGGCC 
GACGAGAAGA TGAAGCAGCT CACAGAGGAG ACGGAGGTGC TGGCACATGA GATCGAGCGG 
CTGCAGATGG AGATGAAGGA GGACGACGTT TCTTTTCTCA TGAAACACAA GAGCCGAAAA 
CGCCGACTCT TCTGCACCAT GGAGCCAGAG CCAGTCCAGC CCGGCATGCT TATCGATGTC 
TGCAAGTACC TGGGCTCCCT GCAGTACCGC GTCTGGAAGA AGATGCTTGC ATCTGTGGAA 
TCTGTACCCT TCAGCTTTGA CCCCAACACC GCAGCTGGCT GGCTCTCCGT GTCTGACGAC 
CTCACCAGCG TCACCAACCA TGGCTACCGC GTGCAGGTGG AGAACCCGGA ACGCTTCTCC 
TCGGCGCCCT GCCTGCTGGG CTCCCGTGTC TTCTCACAGG GCTCGCACGC CTGGGAGGTG 
GCCCTTGGGG GGCTGCAGAG CTGGAGGGTG GGCGTGGTAC GTGTGCGCCA GGACTCGGGC 
GCTGAGGGCC ACTCACACAG CTGCTACCAC GACACACGCT CGGGCTTCTG GTATGTCTGC 
CGCACGCAGG GCGTGGAGGG GGACCACTGC GTGACCTCGG ACCCAGCCAC GTCGCCCCTG 
GTCCTGGCCA TCCCACGCCG CCTGCGTGTG GAGCTGGAGT GTGAGGAGGG CGAGCTGTCT 
TTCTATGACG CGGAGCGCCA CTGCCACCTG TACACCTTCC ACGCCCGCTT TGGGGAGGTT 
CGCCCCTACT TCTACCTGGG GGGTGCACGG GGCGCCGGGC CTCCAGAGCC TTTGCGCATC 
TGCCCCTTGC ACATCAGTGT CAAGGAAGAA CTGGATGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_741983.2
CDS	85..1566
Translation	MERSPDVSPGPSRSFKEELLCAVCYDPFRDAVTLRCGHNFCRGCVSRCWEVQVSPTCPVCKDRASPADLRTNHTLNNLVEKLLREEAEGARWTSYRFSRVCRLHRGQLSLFCLEDKELLCCSCQADPRHQGHRVQPVKDTAHDFRAKCRNMEHALREKAKAFWAMRRSYEAIAKHNQVEAAWLEGRIRQEFDKLREFLRVEEQAILDAMAEETRQKQLLADEKMKQLTEETEVLAHEIERLQMEMKEDDVSFLMKHKSRKRRLFCTMEPEPVQPGMLIDVCKYLGSLQYRVWKKMLASVESVPFSFDPNTAAGWLSVSDDLTSVTNHGYRVQVENPERFSSAPCLLGSRVFSQGSHAWEVALGGLQSWRVGVVRVRQDSGAEGHSHSCYHDTRSGFWYVCRTQGVEGDHCVTSDPATSPLVLAIPRRLRVELECEEGELSFYDAERHCHLYTFHARFGEVRPYFYLGGARGAGPPEPLRICPLHISVKEELDG

Target ORF information:

RefSeq Version	NM_171982.4
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 35 (TRIM35), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_171982.4

ORF Insert Sequence:

ATGGAGCGGA GTCCCGACGT GTCCCCCGGG CCTTCCCGCT CCTTCAAGGA GGAGTTGCTC 
TGCGCCGTCT GCTACGACCC CTTCCGCGAC GCAGTCACTC TGCGCTGCGG CCACAACTTC 
TGCCGCGGGT GCGTGAGCCG CTGCTGGGAG GTGCAGGTGT CGCCCACCTG CCCAGTGTGC 
AAAGACCGCG CGTCACCCGC CGACCTGCGC ACCAACCACA CCCTCAACAA CCTGGTGGAG 
AAGCTGCTGC GCGAGGAGGC CGAGGGCGCG CGCTGGACCA GCTACCGCTT CTCGCGTGTC 
TGCCGCCTGC ACCGCGGACA GCTCAGCCTC TTCTGCCTCG AGGACAAGGA GCTGCTGTGC 
TGCTCCTGCC AGGCCGACCC CCGACACCAG GGGCACCGCG TGCAGCCGGT GAAGGACACT 
GCCCACGACT TTCGGGCCAA GTGCAGGAAC ATGGAGCATG CACTGCGGGA GAAGGCCAAG 
GCCTTCTGGG CCATGCGGCG CTCCTATGAG GCCATCGCCA AGCACAATCA GGTGGAGGCT 
GCATGGCTGG AAGGCCGGAT CCGGCAGGAG TTTGATAAGC TTCGCGAGTT CTTGAGAGTG 
GAGGAGCAGG CCATTCTGGA TGCCATGGCC GAGGAGACAA GGCAGAAGCA ACTTCTGGCC 
GACGAGAAGA TGAAGCAGCT CACAGAGGAG ACGGAGGTGC TGGCACATGA GATCGAGCGG 
CTGCAGATGG AGATGAAGGA GGACGACGTT TCTTTTCTCA TGAAACACAA GAGCCGAAAA 
CGCCGACTCT TCTGCACCAT GGAGCCAGAG CCAGTCCAGC CCGGCATGCT TATCGATGTC 
TGCAAGTACC TGGGCTCCCT GCAGTACCGC GTCTGGAAGA AGATGCTTGC ATCTGTGGAA 
TCTGTACCCT TCAGCTTTGA CCCCAACACC GCAGCTGGCT GGCTCTCCGT GTCTGACGAC 
CTCACCAGCG TCACCAACCA TGGCTACCGC GTGCAGGTGG AGAACCCGGA ACGCTTCTCC 
TCGGCGCCCT GCCTGCTGGG CTCCCGTGTC TTCTCACAGG GCTCGCACGC CTGGGAGGTG 
GCCCTTGGGG GGCTGCAGAG CTGGAGGGTG GGCGTGGTAC GTGTGCGCCA GGACTCGGGC 
GCTGAGGGCC ACTCACACAG CTGCTACCAC GACACACGCT CGGGCTTCTG GTATGTCTGC 
CGCACGCAGG GCGTGGAGGG GGACCACTGC GTGACCTCGG ACCCAGCCAC GTCGCCCCTG 
GTCCTGGCCA TCCCACGCCG CCTGCGTGTG GAGCTGGAGT GTGAGGAGGG CGAGCTGTCT 
TTCTATGACG CGGAGCGCCA CTGCCACCTG TACACCTTCC ACGCCCGCTT TGGGGAGGTT 
CGCCCCTACT TCTACCTGGG GGGTGCACGG GGCGCCGGGC CTCCAGAGCC TTTGCGCATC 
TGCCCCTTGC ACATCAGTGT CAAGGAAGAA CTGGATGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

miR-4417 Targets Tripartite Motif-Containing 35 (TRIM35) and Regulates Pyruvate Kinase Muscle 2 (PKM2) Phosphorylation to Promote Proliferation and Suppress Apoptosis in Hepatocellular Carcinoma Cells.
Medical science monitor : international medical journal of experimental and clinical research231741-1750(2017 Apr)
Song L,Zhang W,Chang Z,Pan Y,Zong H,Fan Q,Wang L

TRIM35 Interacts with pyruvate kinase isoform M2 to suppress the Warburg effect and tumorigenicity in hepatocellular carcinoma.
Oncogene34(30)3946-56(2015 Jul)
Chen Z,Wang Z,Guo W,Zhang Z,Zhao F,Zhao Y,Jia D,Ding J,Wang H,Yao M,He X

Co-expression of PKM2 and TRIM35 predicts survival and recurrence in hepatocellular carcinoma.
Oncotarget6(4)2538-48(2015 Feb)
Chen Z,Lu X,Wang Z,Jin G,Wang Q,Chen D,Chen T,Li J,Fan J,Cong W,Gao Q,He X

TRIM E3 ligases interfere with early and late stages of the retroviral life cycle.
PLoS pathogens4(2)e16(2008 Feb)
Uchil PD,Quinlan BD,Chan WT,Luna JM,Mothes W

HLS5, a novel RBCC (ring finger, B box, coiled-coil) family member isolated from a hemopoietic lineage switch, is a candidate tumor suppressor.
The Journal of biological chemistry279(9)8181-9(2004 Feb)
Lalonde JP,Lim R,Ingley E,Tilbrook PA,Thompson MJ,McCulloch R,Beaumont JG,Wicking C,Eyre HJ,Sutherland GR,Howe K,Solomon E,Williams JH,Klinken SP

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TRIM35 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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