EEF2KMT cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EEF2KMT
Entrez Gene ID	196483
Full Name	eukaryotic elongation factor 2 lysine methyltransferase
Synonyms	EFM3,FAM86A,SB153,eEF2-KMT
General protein information	Preferred Names eukaryotic elongation factor 2 lysine methyltransferase Names protein-lysine N-methyltransferase EEF2KMT eEF2-lysine methyltransferase family with sequence similarity 86, member A protein FAM86A putative protein N-methyltransferase FAM86A
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p13.3
Disorder MIM:	615263

mRNA and Protein(s)

mRNA	Protein	Name
NM_201400.3	NP_958802.1	protein-lysine N-methyltransferase EEF2KMT isoform 1
NM_201598.3	NP_963892.1	protein-lysine N-methyltransferase EEF2KMT isoform 2
NM_001289029.1	NP_001275958.1	protein-lysine N-methyltransferase EEF2KMT isoform 3
XM_005255157.4	XP_005255214.1	protein-lysine N-methyltransferase EEF2KMT isoform X1
XM_011522404.2	XP_011520706.1	protein-lysine N-methyltransferase EEF2KMT isoform X2
XM_005255158.4	XP_005255215.1	protein-lysine N-methyltransferase EEF2KMT isoform X2
NM_201400.4	NP_958802.1	protein-lysine N-methyltransferase EEF2KMT isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	FAM86A	NP_958802.1
Macaca mulatta (Rhesus monkey)	FAM86A	XP_001100213.1
Danio rerio (zebrafish)	fam86a	NP_001006058.1
Mus musculus (house mouse)	Fam86	NP_081722.1
Canis lupus familiaris (dog)	FAM86A	XP_005621633.1
Rattus norvegicus (Norway rat)	Fam86a	NP_001100445.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004255	XP_313174.3
Pan troglodytes (chimpanzee)	LOC742623	XP_003950240.1
Bos taurus (cattle)	FAM86A	NP_001157410.1
Drosophila melanogaster (fruit fly)	CG7889	NP_573368.2
Gallus gallus (chicken)	FAM86A	NP_001025798.1
Xenopus tropicalis (tropical clawed frog)	fam86a	XP_002938331.2

Gene Ontology
Bibliography

Related articles in PubMed

Identification and characterization of a novel evolutionarily conserved lysine-specific methyltransferase targeting eukaryotic translation elongation factor 2 (eEF2).
Davydova E, Ho AY, Malecki J, Moen A, Enserink JM, Jakobsson ME, Loenarz C, Falnes PØ
The Journal of biological chemistry289(44)30499-510(2014 Oct)

Exome sequencing identifies titin mutations causing hereditary myopathy with early respiratory failure (HMERF) in families of diverse ethnic origins.
Toro C, Olivé M, Dalakas MC, Sivakumar K, Bilbao JM, Tyndel F, Vidal N, Farrero E, Sambuughin N, Goldfarb LG
BMC neurology1329(2013 Mar)

A newly uncovered group of distantly related lysine methyltransferases preferentially interact with molecular chaperones to regulate their activity.
Cloutier P, Lavallée-Adam M, Faubert D, Blanchette M, Coulombe B
PLoS genetics9(1)e1003210(2013)

Genome-wide association for abdominal subcutaneous and visceral adipose reveals a novel locus for visceral fat in women.
Fox CS, Liu Y, White CC, Feitosa M, Smith AV, Heard-Costa N, Lohman K, , , , Johnson AD, Foster MC, Greenawalt DM, Griffin P, Ding J, Newman AB, Tylavsky F, Miljkovic I, Kritchevsky SB, Launer L, Garcia M, Eiriksdottir G, Carr JJ, Gudnason V, Harris TB, Cupples LA, Borecki IB
PLoS genetics8(5)e1002695(2012)

Isolation of novel and known genes from a human fetal cochlear cDNA library using subtractive hybridization and differential screening.
Robertson NG, Khetarpal U, Gutiérrez-Espeleta GA, Bieber FR, Morton CC
Genomics23(1)42-50(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EEF2KMT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EEF2KMT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02913	NM_201598.3 Latest version!	Homo sapiens eukaryotic elongation factor 2 lysine methyltransferase (EEF2KMT), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu06547	NM_001289029.1 Latest version!	Homo sapiens eukaryotic elongation factor 2 lysine methyltransferase (EEF2KMT), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu108612	NM_201400.4 Latest version!	Homo sapiens eukaryotic elongation factor 2 lysine methyltransferase (EEF2KMT), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu48779	XM_005255157.4 Latest version!	Homo sapiens eukaryotic elongation factor 2 lysine methyltransferase (EEF2KMT), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu48780	XM_011522404.2 Latest version!	Homo sapiens eukaryotic elongation factor 2 lysine methyltransferase (EEF2KMT), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu48780	XM_005255158.4 Latest version!	Homo sapiens eukaryotic elongation factor 2 lysine methyltransferase (EEF2KMT), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu02850	NM_201400.3 Latest version!	Homo sapiens eukaryotic elongation factor 2 lysine methyltransferase (EEF2KMT), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$216.30 $309.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EEF2KMT ( NM_201598.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_963892.1 Homo sapiens eukaryotic elongation factor 2 lysine methyltransferase (EEF2KMT), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02913
Clone ID Related Accession (Same CDS sequence)	NM_201598.3
Accession Version	NM_201598.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1530201600000
Organism	Homo sapiens(human)
Product	protein-lysine N-methyltransferase EEF2KMT isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY017433.1, AY037162.1, BI491350.1, BM691620.1, HY265953.1 and AI669435.1. On Jan 10, 2014 this sequence version replaced NM_201598.2. Transcript Variant: This variant (2) lacks an alternate exon in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.81723.1, SRR1803615.46036.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCGCCCG AGGAGAACGC GGGGACCGAA CTCTTGCTGC AGAGTTTCGA GCGCCGCTTC 
CTGGCGGCAC GCACACTGCG CTCCTTCCCC TGGCAGAGCT TAGAAGCAAA GTTAAGAGAC 
TCATCAGATT CTGAGCTGCT GCGGGATATT TTGCACAAGA CTGTGAAGCA TCCTGTGTGT 
GTGAAGCACC CGCCGTCCGT CAAATATGCC CGGTGCTTTC TCTCAGAACT CATCAAAAAG 
CCCTCGGGAG GCTCGGTCAC ACTCTCCGAG AGCACGGCCA TCATCTCCTA CGGTACCACA 
GGCCTGGTCA CATGGGACGC CGCCCTCTAC CTTGCAGAAT GGGCCATCGA GAACCCGGCA 
GTCTTCACTA ACAGGACTGT CCTAGAGCTT GGCAGTGGTG CTGGCCTCAC AGGCCTGGCC 
ATCTGCAAGA TGTGCCGCCC CCGGGCATAC ATCTTCAGCG ACTGTCACAG CCGGGTCCTT 
GAGCAGCTCC GAGGGAATGT CCTTCTCAAT GGCCTCTCAT TAGAGGCAGA CATCACTGCC 
AAGTTAGACA GCCCCAGGGT GACAGTGGCC CAGCTGGACT GGGACGTCGC GACGGTCCAT 
CAGCTCTCTG CCTTCCAGCC AGATGTTGTC ATTGCAGCAG ATGTGCTGTA TTGCCCAGAA 
GCCATCATGT CGCTGGTCGG CGTCCTGCGG AGGCTGGCTG CCTGCCGGGA GCACCAGCGG 
GCTCCTGAGG TCTACGTGGC CTTTACCGTC CGCAACCCAG AGACGTGCCA GCTGTTCACC 
ACCGAGCTAG GCCGGGCCGG GATCAGATGG GAAGTGGAAC CTCGTCATGA GCAGAAACTG 
TTTCCCTACG AAGAGCACTT GGAGATGGCA ATGCTGAATC TCACCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_963892.1
CDS	82..972
Translation	MAPEENAGTELLLQSFERRFLAARTLRSFPWQSLEAKLRDSSDSELLRDILHKTVKHPVCVKHPPSVKYARCFLSELIKKPSGGSVTLSESTAIISYGTTGLVTWDAALYLAEWAIENPAVFTNRTVLELGSGAGLTGLAICKMCRPRAYIFSDCHSRVLEQLRGNVLLNGLSLEADITAKLDSPRVTVAQLDWDVATVHQLSAFQPDVVIAADVLYCPEAIMSLVGVLRRLAACREHQRAPEVYVAFTVRNPETCQLFTTELGRAGIRWEVEPRHEQKLFPYEEHLEMAMLNLTL

Target ORF information:

RefSeq Version	NM_201598.3
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic elongation factor 2 lysine methyltransferase (EEF2KMT), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201598.3

ORF Insert Sequence:

ATGGCGCCCG AGGAGAACGC GGGGACCGAA CTCTTGCTGC AGAGTTTCGA GCGCCGCTTC 
CTGGCGGCAC GCACACTGCG CTCCTTCCCC TGGCAGAGCT TAGAAGCAAA GTTAAGAGAC 
TCATCAGATT CTGAGCTGCT GCGGGATATT TTGCACAAGA CTGTGAAGCA TCCTGTGTGT 
GTGAAGCACC CGCCGTCCGT CAAATATGCC CGGTGCTTTC TCTCAGAACT CATCAAAAAG 
CCCTCGGGAG GCTCGGTCAC ACTCTCCGAG AGCACGGCCA TCATCTCCTA CGGTACCACA 
GGCCTGGTCA CATGGGACGC CGCCCTCTAC CTTGCAGAAT GGGCCATCGA GAACCCGGCA 
GTCTTCACTA ACAGGACTGT CCTAGAGCTT GGCAGTGGTG CTGGCCTCAC AGGCCTGGCC 
ATCTGCAAGA TGTGCCGCCC CCGGGCATAC ATCTTCAGCG ACTGTCACAG CCGGGTCCTT 
GAGCAGCTCC GAGGGAATGT CCTTCTCAAT GGCCTCTCAT TAGAGGCAGA CATCACTGCC 
AAGTTAGACA GCCCCAGGGT GACAGTGGCC CAGCTGGACT GGGACGTCGC GACGGTCCAT 
CAGCTCTCTG CCTTCCAGCC AGATGTTGTC ATTGCAGCAG ATGTGCTGTA TTGCCCAGAA 
GCCATCATGT CGCTGGTCGG CGTCCTGCGG AGGCTGGCTG CCTGCCGGGA GCACCAGCGG 
GCTCCTGAGG TCTACGTGGC CTTTACCGTC CGCAACCCAG AGACGTGCCA GCTGTTCACC 
ACCGAGCTAG GCCGGGCCGG GATCAGATGG GAAGTGGAAC CTCGTCATGA GCAGAAACTG 
TTTCCCTACG AAGAGCACTT GGAGATGGCA ATGCTGAATC TCACCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEF2KMT ( NM_001289029.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001275958.1 Homo sapiens eukaryotic elongation factor 2 lysine methyltransferase (EEF2KMT), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu06547
Clone ID Related Accession (Same CDS sequence)	NM_001289029.1
Accession Version	NM_001289029.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1530201600000
Organism	Homo sapiens(human)
Product	protein-lysine N-methyltransferase EEF2KMT isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY017433.1, AL522535.3, AY037162.1, BI491350.1, BM691620.1, HY265953.1 and AI669435.1. Transcript Variant: This variant (3) lacks two alternate exons in the coding region compared to variant 1. The resulting protein (isoform 3) is shorter but has the same N- and C-termini compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.256595.1, SRR1163658.230448.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCGCCCG AGGAGAACGC GGGGACCGAA CTCTTGCTGC AGAGTTTCGA GCGCCGCTTC 
CTGGCGGCAC GCACACTGCG CTCCTTCCCC TGGCAGAGCT TAGAAGCAAA GTTAAGAGAC 
TCATCAGATT CTGAGCTGCT GCGGGATATT TTGCACAAGC CCTCGGGAGG CTCGGTCACA 
CTCTCCGAGA GCACGGCCAT CATCTCCTAC GGTACCACAG GCCTGGTCAC ATGGGACGCC 
GCCCTCTACC TTGCAGAATG GGCCATCGAG AACCCGGCAG TCTTCACTAA CAGGACTGTC 
CTAGAGCTTG GCAGTGGTGC TGGCCTCACA GGCCTGGCCA TCTGCAAGAT GTGCCGCCCC 
CGGGCATACA TCTTCAGCGA CTGTCACAGC CGGGTCCTTG AGCAGCTCCG AGGGAATGTC 
CTTCTCAATG GCCTCTCATT AGAGGCAGAC ATCACTGCCA AGTTAGACAG CCCCAGGGTG 
ACAGTGGCCC AGCTGGACTG GGACGTCGCG ACGGTCCATC AGCTCTCTGC CTTCCAGCCA 
GATGTTGTCA TTGCAGCAGA TGTGCTGTAT TGCCCAGAAG CCATCATGTC GCTGGTCGGC 
GTCCTGCGGA GGCTGGCTGC CTGCCGGGAG CACCAGCGGG CTCCTGAGGT CTACGTGGCC 
TTTACCGTCC GCAACCCAGA GACGTGCCAG CTGTTCACCA CCGAGCTAGG CCGGGCCGGG 
ATCAGATGGG AAGTGGAACC TCGTCATGAG CAGAAACTGT TTCCCTACGA AGAGCACTTG 
GAGATGGCAA TGCTGAATCT CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001275958.1
CDS	82..891
Translation	MAPEENAGTELLLQSFERRFLAARTLRSFPWQSLEAKLRDSSDSELLRDILHKPSGGSVTLSESTAIISYGTTGLVTWDAALYLAEWAIENPAVFTNRTVLELGSGAGLTGLAICKMCRPRAYIFSDCHSRVLEQLRGNVLLNGLSLEADITAKLDSPRVTVAQLDWDVATVHQLSAFQPDVVIAADVLYCPEAIMSLVGVLRRLAACREHQRAPEVYVAFTVRNPETCQLFTTELGRAGIRWEVEPRHEQKLFPYEEHLEMAMLNLTL

Target ORF information:

RefSeq Version	NM_001289029.1
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic elongation factor 2 lysine methyltransferase (EEF2KMT), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289029.1

ORF Insert Sequence:

ATGGCGCCCG AGGAGAACGC GGGGACCGAA CTCTTGCTGC AGAGTTTCGA GCGCCGCTTC 
CTGGCGGCAC GCACACTGCG CTCCTTCCCC TGGCAGAGCT TAGAAGCAAA GTTAAGAGAC 
TCATCAGATT CTGAGCTGCT GCGGGATATT TTGCACAAGC CCTCGGGAGG CTCGGTCACA 
CTCTCCGAGA GCACGGCCAT CATCTCCTAC GGTACCACAG GCCTGGTCAC ATGGGACGCC 
GCCCTCTACC TTGCAGAATG GGCCATCGAG AACCCGGCAG TCTTCACTAA CAGGACTGTC 
CTAGAGCTTG GCAGTGGTGC TGGCCTCACA GGCCTGGCCA TCTGCAAGAT GTGCCGCCCC 
CGGGCATACA TCTTCAGCGA CTGTCACAGC CGGGTCCTTG AGCAGCTCCG AGGGAATGTC 
CTTCTCAATG GCCTCTCATT AGAGGCAGAC ATCACTGCCA AGTTAGACAG CCCCAGGGTG 
ACAGTGGCCC AGCTGGACTG GGACGTCGCG ACGGTCCATC AGCTCTCTGC CTTCCAGCCA 
GATGTTGTCA TTGCAGCAGA TGTGCTGTAT TGCCCAGAAG CCATCATGTC GCTGGTCGGC 
GTCCTGCGGA GGCTGGCTGC CTGCCGGGAG CACCAGCGGG CTCCTGAGGT CTACGTGGCC 
TTTACCGTCC GCAACCCAGA GACGTGCCAG CTGTTCACCA CCGAGCTAGG CCGGGCCGGG 
ATCAGATGGG AAGTGGAACC TCGTCATGAG CAGAAACTGT TTCCCTACGA AGAGCACTTG 
GAGATGGCAA TGCTGAATCT CACCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEF2KMT ( NM_201400.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_958802.1 Homo sapiens eukaryotic elongation factor 2 lysine methyltransferase (EEF2KMT), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu108612
Clone ID Related Accession (Same CDS sequence)	NM_201400.4
Accession Version	NM_201400.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571846400000
Organism	Homo sapiens(human)
Product	protein-lysine N-methyltransferase EEF2KMT isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC026458.8. On May 17, 2019 this sequence version replaced NM_201400.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: SRR1803617.85008.1, SRR1660807.212373.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000427587.9/ ENSP00000398502.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGGCGCCCG AGGAGAACGC GGGGACCGAA CTCTTGCTGC AGAGTTTCGA GCGCCGCTTC 
CTGGCGGCAC GCACACTGCG CTCCTTCCCC TGGCAGAGCT TAGAAGCAAA GTTAAGAGAC 
TCATCAGATT CTGAGCTGCT GCGGGATATT TTGCACAAGA CTGTGAAGCA TCCTGTGTGT 
GTGAAGCACC CGCCGTCCGT CAAATATGCC CGGTGCTTTC TCTCAGAACT CATCAAAAAG 
CACGAGGCTG TCCACACAGA GCCTTTGGAC GAGCTGTATG AAGCGCTGGC GGAGACCCTG 
ATGGCCAAGG AGTCCACCCA GGGCCACCGG AGCTATTTGC TGCCCTCGGG AGGCTCGGTC 
ACACTCTCCG AGAGCACGGC CATCATCTCC TACGGTACCA CAGGCCTGGT CACATGGGAC 
GCCGCCCTCT ACCTTGCAGA ATGGGCCATC GAGAACCCGG CAGTCTTCAC TAACAGGACT 
GTCCTAGAGC TTGGCAGTGG TGCTGGCCTC ACAGGCCTGG CCATCTGCAA GATGTGCCGC 
CCCCGGGCAT ACATCTTCAG CGACTGTCAC AGCCGGGTCC TTGAGCAGCT CCGAGGGAAT 
GTCCTTCTCA ATGGCCTCTC ATTAGAGGCA GACATCACTG CCAAGTTAGA CAGCCCCAGG 
GTGACAGTGG CCCAGCTGGA CTGGGACGTC GCGACGGTCC ATCAGCTCTC TGCCTTCCAG 
CCAGATGTTG TCATTGCAGC AGATGTGCTG TATTGCCCAG AAGCCATCAT GTCGCTGGTC 
GGGGTCCTGC GGAGGCTGGC TGCCTGCCGG GAGCACCAGC GGGCTCCTGA GGTCTACGTG 
GCCTTTACCG TCCGCAACCC AGAGACGTGC CAGCTGTTCA CCACCGAGCT AGGCCGGGCC 
GGGATCAGAT GGGAAGTGGA ACCTCGTCAT GAGCAGAAAC TGTTTCCCTA CGAAGAGCAC 
TTGGAGATGG CAATGCTGAA TCTCACCCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_958802.1
CDS	57..1049
Translation	MAPEENAGTELLLQSFERRFLAARTLRSFPWQSLEAKLRDSSDSELLRDILHKTVKHPVCVKHPPSVKYARCFLSELIKKHEAVHTEPLDELYEALAETLMAKESTQGHRSYLLPSGGSVTLSESTAIISYGTTGLVTWDAALYLAEWAIENPAVFTNRTVLELGSGAGLTGLAICKMCRPRAYIFSDCHSRVLEQLRGNVLLNGLSLEADITAKLDSPRVTVAQLDWDVATVHQLSAFQPDVVIAADVLYCPEAIMSLVGVLRRLAACREHQRAPEVYVAFTVRNPETCQLFTTELGRAGIRWEVEPRHEQKLFPYEEHLEMAMLNLTL

Target ORF information:

RefSeq Version	NM_201400.4
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic elongation factor 2 lysine methyltransferase (EEF2KMT), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201400.4

ORF Insert Sequence:

ATGGCGCCCG AGGAGAACGC GGGGACCGAA CTCTTGCTGC AGAGTTTCGA GCGCCGCTTC 
CTGGCGGCAC GCACACTGCG CTCCTTCCCC TGGCAGAGCT TAGAAGCAAA GTTAAGAGAC 
TCATCAGATT CTGAGCTGCT GCGGGATATT TTGCACAAGA CTGTGAAGCA TCCTGTGTGT 
GTGAAGCACC CGCCGTCCGT CAAATATGCC CGGTGCTTTC TCTCAGAACT CATCAAAAAG 
CACGAGGCTG TCCACACAGA GCCTTTGGAC GAGCTGTATG AAGCGCTGGC GGAGACCCTG 
ATGGCCAAGG AGTCCACCCA GGGCCACCGG AGCTATTTGC TGCCCTCGGG AGGCTCGGTC 
ACACTCTCCG AGAGCACGGC CATCATCTCC TACGGTACCA CAGGCCTGGT CACATGGGAC 
GCCGCCCTCT ACCTTGCAGA ATGGGCCATC GAGAACCCGG CAGTCTTCAC TAACAGGACT 
GTCCTAGAGC TTGGCAGTGG TGCTGGCCTC ACAGGCCTGG CCATCTGCAA GATGTGCCGC 
CCCCGGGCAT ACATCTTCAG CGACTGTCAC AGCCGGGTCC TTGAGCAGCT CCGAGGGAAT 
GTCCTTCTCA ATGGCCTCTC ATTAGAGGCA GACATCACTG CCAAGTTAGA CAGCCCCAGG 
GTGACAGTGG CCCAGCTGGA CTGGGACGTC GCGACGGTCC ATCAGCTCTC TGCCTTCCAG 
CCAGATGTTG TCATTGCAGC AGATGTGCTG TATTGCCCAG AAGCCATCAT GTCGCTGGTC 
GGGGTCCTGC GGAGGCTGGC TGCCTGCCGG GAGCACCAGC GGGCTCCTGA GGTCTACGTG 
GCCTTTACCG TCCGCAACCC AGAGACGTGC CAGCTGTTCA CCACCGAGCT AGGCCGGGCC 
GGGATCAGAT GGGAAGTGGA ACCTCGTCAT GAGCAGAAAC TGTTTCCCTA CGAAGAGCAC 
TTGGAGATGG CAATGCTGAA TCTCACCCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEF2KMT ( XM_005255157.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005255214.1 Homo sapiens eukaryotic elongation factor 2 lysine methyltransferase (EEF2KMT), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu48779
Clone ID Related Accession (Same CDS sequence)	XM_005255157.4
Accession Version	XM_005255157.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	protein-lysine N-methyltransferase EEF2KMT isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005255157.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCCG AGGAGAACGC GGGGACCGAA CTCTTGCTGC AGAGTTTCGA GCGCCGCTTC 
CTGGCGGCAC GCACACTGCG CTCCTTCCCC TGGCAGAGCT TAGAAGCAAA GTTAAGAGAC 
TCATCAGATT CTGAGCTGCT GCGGGATATT TTGCACAAGC ACGAGGCTGT CCACACAGAG 
CCTTTGGACG AGCTGTATGA AGCGCTGGCG GAGACCCTGA TGGCCAAGGA GTCCACCCAG 
GGCCACCGGA GCTATTTGCT GCCCTCGGGA GGCTCGGTCA CACTCTCCGA GAGCACGGCC 
ATCATCTCCT ACGGTACCAC AGGCCTGGTC ACATGGGACG CCGCCCTCTA CCTTGCAGAA 
TGGGCCATCG AGAACCCGGC AGTCTTCACT AACAGGACTG TCCTAGAGCT TGGCAGTGGT 
GCTGGCCTCA CAGGCCTGGC CATCTGCAAG ATGTGCCGCC CCCGGGCATA CATCTTCAGC 
GACTGTCACA GCCGGGTCCT TGAGCAGCTC CGAGGGAATG TCCTTCTCAA TGGCCTCTCA 
TTAGAGGCAG ACATCACTGC CAAGTTAGAC AGCCCCAGGG TGACAGTGGC CCAGCTGGAC 
TGGGACGTCG CGACGGTCCA TCAGCTCTCT GCCTTCCAGC CAGATGTTGT CATTGCAGCA 
GATGTGCTGT ATTGCCCAGA AGCCATCATG TCGCTGGTCG GGGTCCTGCG GAGGCTGGCT 
GCCTGCCGGG AGCACCAGCG GGCTCCTGAG GTCTACGTGG CCTTTACCGT CCGCAACCCA 
GAGACGTGCC AGCTGTTCAC CACCGAGCTA GGCCGGGCCG GGATCAGATG GGAAGTGGAA 
CCTCGTCATG AGCAGAAACT GTTTCCCTAC GAAGAGCACT TGGAGATGGC AATGCTGAAT 
CTCACCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005255214.1
CDS	55..966
Translation	MAPEENAGTELLLQSFERRFLAARTLRSFPWQSLEAKLRDSSDSELLRDILHKHEAVHTEPLDELYEALAETLMAKESTQGHRSYLLPSGGSVTLSESTAIISYGTTGLVTWDAALYLAEWAIENPAVFTNRTVLELGSGAGLTGLAICKMCRPRAYIFSDCHSRVLEQLRGNVLLNGLSLEADITAKLDSPRVTVAQLDWDVATVHQLSAFQPDVVIAADVLYCPEAIMSLVGVLRRLAACREHQRAPEVYVAFTVRNPETCQLFTTELGRAGIRWEVEPRHEQKLFPYEEHLEMAMLNLTL

Target ORF information:

RefSeq Version	XM_005255157.4
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic elongation factor 2 lysine methyltransferase (EEF2KMT), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005255157.4

ORF Insert Sequence:

ATGGCGCCCG AGGAGAACGC GGGGACCGAA CTCTTGCTGC AGAGTTTCGA GCGCCGCTTC 
CTGGCGGCAC GCACACTGCG CTCCTTCCCC TGGCAGAGCT TAGAAGCAAA GTTAAGAGAC 
TCATCAGATT CTGAGCTGCT GCGGGATATT TTGCACAAGC ACGAGGCTGT CCACACAGAG 
CCTTTGGACG AGCTGTATGA AGCGCTGGCG GAGACCCTGA TGGCCAAGGA GTCCACCCAG 
GGCCACCGGA GCTATTTGCT GCCCTCGGGA GGCTCGGTCA CACTCTCCGA GAGCACGGCC 
ATCATCTCCT ACGGTACCAC AGGCCTGGTC ACATGGGACG CCGCCCTCTA CCTTGCAGAA 
TGGGCCATCG AGAACCCGGC AGTCTTCACT AACAGGACTG TCCTAGAGCT TGGCAGTGGT 
GCTGGCCTCA CAGGCCTGGC CATCTGCAAG ATGTGCCGCC CCCGGGCATA CATCTTCAGC 
GACTGTCACA GCCGGGTCCT TGAGCAGCTC CGAGGGAATG TCCTTCTCAA TGGCCTCTCA 
TTAGAGGCAG ACATCACTGC CAAGTTAGAC AGCCCCAGGG TGACAGTGGC CCAGCTGGAC 
TGGGACGTCG CGACGGTCCA TCAGCTCTCT GCCTTCCAGC CAGATGTTGT CATTGCAGCA 
GATGTGCTGT ATTGCCCAGA AGCCATCATG TCGCTGGTCG GGGTCCTGCG GAGGCTGGCT 
GCCTGCCGGG AGCACCAGCG GGCTCCTGAG GTCTACGTGG CCTTTACCGT CCGCAACCCA 
GAGACGTGCC AGCTGTTCAC CACCGAGCTA GGCCGGGCCG GGATCAGATG GGAAGTGGAA 
CCTCGTCATG AGCAGAAACT GTTTCCCTAC GAAGAGCACT TGGAGATGGC AATGCTGAAT 
CTCACCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEF2KMT ( XM_011522404.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011520706.1 Homo sapiens eukaryotic elongation factor 2 lysine methyltransferase (EEF2KMT), transcript variant X3, mRNA.

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CloneID	OHu48780
Clone ID Related Accession (Same CDS sequence)	XM_011522404.2 , XM_005255158.4
Accession Version	XM_011522404.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	protein-lysine N-methyltransferase EEF2KMT isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522404.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGTCCACCCA GGGCCACCGG AGCTATTTGC TGCCCTCGGG AGGCTCGGTC 
ACACTCTCCG AGAGCACGGC CATCATCTCC TACGGTACCA CAGGCCTGGT CACATGGGAC 
GCCGCCCTCT ACCTTGCAGA ATGGGCCATC GAGAACCCGG CAGTCTTCAC TAACAGGACT 
GTCCTAGAGC TTGGCAGTGG TGCTGGCCTC ACAGGCCTGG CCATCTGCAA GATGTGCCGC 
CCCCGGGCAT ACATCTTCAG CGACTGTCAC AGCCGGGTCC TTGAGCAGCT CCGAGGGAAT 
GTCCTTCTCA ATGGCCTCTC ATTAGAGGCA GACATCACTG CCAAGTTAGA CAGCCCCAGG 
GTGACAGTGG CCCAGCTGGA CTGGGACGTC GCGACGGTCC ATCAGCTCTC TGCCTTCCAG 
CCAGATGTTG TCATTGCAGC AGATGTGCTG TATTGCCCAG AAGCCATCAT GTCGCTGGTC 
GGGGTCCTGC GGAGGCTGGC TGCCTGCCGG GAGCACCAGC GGGCTCCTGA GGTCTACGTG 
GCCTTTACCG TCCGCAACCC AGAGACGTGC CAGCTGTTCA CCACCGAGCT AGGCCGGGCC 
GGGATCAGAT GGGAAGTGGA ACCTCGTCAT GAGCAGAAAC TGTTTCCCTA CGAAGAGCAC 
TTGGAGATGG CAATGCTGAA TCTCACCCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011520706.1
CDS	347..1039
Translation	MAKESTQGHRSYLLPSGGSVTLSESTAIISYGTTGLVTWDAALYLAEWAIENPAVFTNRTVLELGSGAGLTGLAICKMCRPRAYIFSDCHSRVLEQLRGNVLLNGLSLEADITAKLDSPRVTVAQLDWDVATVHQLSAFQPDVVIAADVLYCPEAIMSLVGVLRRLAACREHQRAPEVYVAFTVRNPETCQLFTTELGRAGIRWEVEPRHEQKLFPYEEHLEMAMLNLTL

Target ORF information:

RefSeq Version	XM_011522404.2
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic elongation factor 2 lysine methyltransferase (EEF2KMT), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011522404.2

ORF Insert Sequence:

ATGGCCAAGG AGTCCACCCA GGGCCACCGG AGCTATTTGC TGCCCTCGGG AGGCTCGGTC 
ACACTCTCCG AGAGCACGGC CATCATCTCC TACGGTACCA CAGGCCTGGT CACATGGGAC 
GCCGCCCTCT ACCTTGCAGA ATGGGCCATC GAGAACCCGG CAGTCTTCAC TAACAGGACT 
GTCCTAGAGC TTGGCAGTGG TGCTGGCCTC ACAGGCCTGG CCATCTGCAA GATGTGCCGC 
CCCCGGGCAT ACATCTTCAG CGACTGTCAC AGCCGGGTCC TTGAGCAGCT CCGAGGGAAT 
GTCCTTCTCA ATGGCCTCTC ATTAGAGGCA GACATCACTG CCAAGTTAGA CAGCCCCAGG 
GTGACAGTGG CCCAGCTGGA CTGGGACGTC GCGACGGTCC ATCAGCTCTC TGCCTTCCAG 
CCAGATGTTG TCATTGCAGC AGATGTGCTG TATTGCCCAG AAGCCATCAT GTCGCTGGTC 
GGGGTCCTGC GGAGGCTGGC TGCCTGCCGG GAGCACCAGC GGGCTCCTGA GGTCTACGTG 
GCCTTTACCG TCCGCAACCC AGAGACGTGC CAGCTGTTCA CCACCGAGCT AGGCCGGGCC 
GGGATCAGAT GGGAAGTGGA ACCTCGTCAT GAGCAGAAAC TGTTTCCCTA CGAAGAGCAC 
TTGGAGATGG CAATGCTGAA TCTCACCCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEF2KMT ( XM_005255158.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005255215.1 Homo sapiens eukaryotic elongation factor 2 lysine methyltransferase (EEF2KMT), transcript variant X2, mRNA.

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CloneID	OHu48780
Clone ID Related Accession (Same CDS sequence)	XM_011522404.2 , XM_005255158.4
Accession Version	XM_005255158.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	protein-lysine N-methyltransferase EEF2KMT isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005255158.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGTCCACCCA GGGCCACCGG AGCTATTTGC TGCCCTCGGG AGGCTCGGTC 
ACACTCTCCG AGAGCACGGC CATCATCTCC TACGGTACCA CAGGCCTGGT CACATGGGAC 
GCCGCCCTCT ACCTTGCAGA ATGGGCCATC GAGAACCCGG CAGTCTTCAC TAACAGGACT 
GTCCTAGAGC TTGGCAGTGG TGCTGGCCTC ACAGGCCTGG CCATCTGCAA GATGTGCCGC 
CCCCGGGCAT ACATCTTCAG CGACTGTCAC AGCCGGGTCC TTGAGCAGCT CCGAGGGAAT 
GTCCTTCTCA ATGGCCTCTC ATTAGAGGCA GACATCACTG CCAAGTTAGA CAGCCCCAGG 
GTGACAGTGG CCCAGCTGGA CTGGGACGTC GCGACGGTCC ATCAGCTCTC TGCCTTCCAG 
CCAGATGTTG TCATTGCAGC AGATGTGCTG TATTGCCCAG AAGCCATCAT GTCGCTGGTC 
GGGGTCCTGC GGAGGCTGGC TGCCTGCCGG GAGCACCAGC GGGCTCCTGA GGTCTACGTG 
GCCTTTACCG TCCGCAACCC AGAGACGTGC CAGCTGTTCA CCACCGAGCT AGGCCGGGCC 
GGGATCAGAT GGGAAGTGGA ACCTCGTCAT GAGCAGAAAC TGTTTCCCTA CGAAGAGCAC 
TTGGAGATGG CAATGCTGAA TCTCACCCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005255215.1
CDS	428..1120
Translation	MAKESTQGHRSYLLPSGGSVTLSESTAIISYGTTGLVTWDAALYLAEWAIENPAVFTNRTVLELGSGAGLTGLAICKMCRPRAYIFSDCHSRVLEQLRGNVLLNGLSLEADITAKLDSPRVTVAQLDWDVATVHQLSAFQPDVVIAADVLYCPEAIMSLVGVLRRLAACREHQRAPEVYVAFTVRNPETCQLFTTELGRAGIRWEVEPRHEQKLFPYEEHLEMAMLNLTL

Target ORF information:

RefSeq Version	XM_005255158.4
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic elongation factor 2 lysine methyltransferase (EEF2KMT), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005255158.4

ORF Insert Sequence:

ATGGCCAAGG AGTCCACCCA GGGCCACCGG AGCTATTTGC TGCCCTCGGG AGGCTCGGTC 
ACACTCTCCG AGAGCACGGC CATCATCTCC TACGGTACCA CAGGCCTGGT CACATGGGAC 
GCCGCCCTCT ACCTTGCAGA ATGGGCCATC GAGAACCCGG CAGTCTTCAC TAACAGGACT 
GTCCTAGAGC TTGGCAGTGG TGCTGGCCTC ACAGGCCTGG CCATCTGCAA GATGTGCCGC 
CCCCGGGCAT ACATCTTCAG CGACTGTCAC AGCCGGGTCC TTGAGCAGCT CCGAGGGAAT 
GTCCTTCTCA ATGGCCTCTC ATTAGAGGCA GACATCACTG CCAAGTTAGA CAGCCCCAGG 
GTGACAGTGG CCCAGCTGGA CTGGGACGTC GCGACGGTCC ATCAGCTCTC TGCCTTCCAG 
CCAGATGTTG TCATTGCAGC AGATGTGCTG TATTGCCCAG AAGCCATCAT GTCGCTGGTC 
GGGGTCCTGC GGAGGCTGGC TGCCTGCCGG GAGCACCAGC GGGCTCCTGA GGTCTACGTG 
GCCTTTACCG TCCGCAACCC AGAGACGTGC CAGCTGTTCA CCACCGAGCT AGGCCGGGCC 
GGGATCAGAT GGGAAGTGGA ACCTCGTCAT GAGCAGAAAC TGTTTCCCTA CGAAGAGCAC 
TTGGAGATGG CAATGCTGAA TCTCACCCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEF2KMT ( NM_201400.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_958802.1 Homo sapiens eukaryotic elongation factor 2 lysine methyltransferase (EEF2KMT), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02850
Clone ID Related Accession (Same CDS sequence)	NM_201400.3
Accession Version	NM_201400.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1530201600000
Organism	Homo sapiens(human)
Product	protein-lysine N-methyltransferase EEF2KMT isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HY017433.1, BC010084.2, AY037162.1, BI491350.1, BM691620.1, HY265953.1 and AI669435.1. On Jan 10, 2014 this sequence version replaced NM_201400.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: SRR1803613.77344.1, SRR1803617.85008.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCGCCCG AGGAGAACGC GGGGACCGAA CTCTTGCTGC AGAGTTTCGA GCGCCGCTTC 
CTGGCGGCAC GCACACTGCG CTCCTTCCCC TGGCAGAGCT TAGAAGCAAA GTTAAGAGAC 
TCATCAGATT CTGAGCTGCT GCGGGATATT TTGCACAAGA CTGTGAAGCA TCCTGTGTGT 
GTGAAGCACC CGCCGTCCGT CAAATATGCC CGGTGCTTTC TCTCAGAACT CATCAAAAAG 
CACGAGGCTG TCCACACAGA GCCTTTGGAC GAGCTGTATG AAGCGCTGGC GGAGACCCTG 
ATGGCCAAGG AGTCCACCCA GGGCCACCGG AGCTATTTGC TGCCCTCGGG AGGCTCGGTC 
ACACTCTCCG AGAGCACGGC CATCATCTCC TACGGTACCA CAGGCCTGGT CACATGGGAC 
GCCGCCCTCT ACCTTGCAGA ATGGGCCATC GAGAACCCGG CAGTCTTCAC TAACAGGACT 
GTCCTAGAGC TTGGCAGTGG TGCTGGCCTC ACAGGCCTGG CCATCTGCAA GATGTGCCGC 
CCCCGGGCAT ACATCTTCAG CGACTGTCAC AGCCGGGTCC TTGAGCAGCT CCGAGGGAAT 
GTCCTTCTCA ATGGCCTCTC ATTAGAGGCA GACATCACTG CCAAGTTAGA CAGCCCCAGG 
GTGACAGTGG CCCAGCTGGA CTGGGACGTC GCGACGGTCC ATCAGCTCTC TGCCTTCCAG 
CCAGATGTTG TCATTGCAGC AGATGTGCTG TATTGCCCAG AAGCCATCAT GTCGCTGGTC 
GGCGTCCTGC GGAGGCTGGC TGCCTGCCGG GAGCACCAGC GGGCTCCTGA GGTCTACGTG 
GCCTTTACCG TCCGCAACCC AGAGACGTGC CAGCTGTTCA CCACCGAGCT AGGCCGGGCC 
GGGATCAGAT GGGAAGTGGA ACCTCGTCAT GAGCAGAAAC TGTTTCCCTA CGAAGAGCAC 
TTGGAGATGG CAATGCTGAA TCTCACCCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_958802.1
CDS	82..1074
Translation	MAPEENAGTELLLQSFERRFLAARTLRSFPWQSLEAKLRDSSDSELLRDILHKTVKHPVCVKHPPSVKYARCFLSELIKKHEAVHTEPLDELYEALAETLMAKESTQGHRSYLLPSGGSVTLSESTAIISYGTTGLVTWDAALYLAEWAIENPAVFTNRTVLELGSGAGLTGLAICKMCRPRAYIFSDCHSRVLEQLRGNVLLNGLSLEADITAKLDSPRVTVAQLDWDVATVHQLSAFQPDVVIAADVLYCPEAIMSLVGVLRRLAACREHQRAPEVYVAFTVRNPETCQLFTTELGRAGIRWEVEPRHEQKLFPYEEHLEMAMLNLTL

Target ORF information:

RefSeq Version	NM_201400.3
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic elongation factor 2 lysine methyltransferase (EEF2KMT), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201400.3

ORF Insert Sequence:

ATGGCGCCCG AGGAGAACGC GGGGACCGAA CTCTTGCTGC AGAGTTTCGA GCGCCGCTTC 
CTGGCGGCAC GCACACTGCG CTCCTTCCCC TGGCAGAGCT TAGAAGCAAA GTTAAGAGAC 
TCATCAGATT CTGAGCTGCT GCGGGATATT TTGCACAAGA CTGTGAAGCA TCCTGTGTGT 
GTGAAGCACC CGCCGTCCGT CAAATATGCC CGGTGCTTTC TCTCAGAACT CATCAAAAAG 
CACGAGGCTG TCCACACAGA GCCTTTGGAC GAGCTGTATG AAGCGCTGGC GGAGACCCTG 
ATGGCCAAGG AGTCCACCCA GGGCCACCGG AGCTATTTGC TGCCCTCGGG AGGCTCGGTC 
ACACTCTCCG AGAGCACGGC CATCATCTCC TACGGTACCA CAGGCCTGGT CACATGGGAC 
GCCGCCCTCT ACCTTGCAGA ATGGGCCATC GAGAACCCGG CAGTCTTCAC TAACAGGACT 
GTCCTAGAGC TTGGCAGTGG TGCTGGCCTC ACAGGCCTGG CCATCTGCAA GATGTGCCGC 
CCCCGGGCAT ACATCTTCAG CGACTGTCAC AGCCGGGTCC TTGAGCAGCT CCGAGGGAAT 
GTCCTTCTCA ATGGCCTCTC ATTAGAGGCA GACATCACTG CCAAGTTAGA CAGCCCCAGG 
GTGACAGTGG CCCAGCTGGA CTGGGACGTC GCGACGGTCC ATCAGCTCTC TGCCTTCCAG 
CCAGATGTTG TCATTGCAGC AGATGTGCTG TATTGCCCAG AAGCCATCAT GTCGCTGGTC 
GGCGTCCTGC GGAGGCTGGC TGCCTGCCGG GAGCACCAGC GGGCTCCTGA GGTCTACGTG 
GCCTTTACCG TCCGCAACCC AGAGACGTGC CAGCTGTTCA CCACCGAGCT AGGCCGGGCC 
GGGATCAGAT GGGAAGTGGA ACCTCGTCAT GAGCAGAAAC TGTTTCCCTA CGAAGAGCAC 
TTGGAGATGG CAATGCTGAA TCTCACCCTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification and characterization of a novel evolutionarily conserved lysine-specific methyltransferase targeting eukaryotic translation elongation factor 2 (eEF2).
The Journal of biological chemistry289(44)30499-510(2014 Oct)
Davydova E,Ho AY,Malecki J,Moen A,Enserink JM,Jakobsson ME,Loenarz C,Falnes PØ

Exome sequencing identifies titin mutations causing hereditary myopathy with early respiratory failure (HMERF) in families of diverse ethnic origins.
BMC neurology1329(2013 Mar)
Toro C,Olivé M,Dalakas MC,Sivakumar K,Bilbao JM,Tyndel F,Vidal N,Farrero E,Sambuughin N,Goldfarb LG

A newly uncovered group of distantly related lysine methyltransferases preferentially interact with molecular chaperones to regulate their activity.
PLoS genetics9(1)e1003210(2013)
Cloutier P,Lavallée-Adam M,Faubert D,Blanchette M,Coulombe B

Genome-wide association for abdominal subcutaneous and visceral adipose reveals a novel locus for visceral fat in women.
PLoS genetics8(5)e1002695(2012)
Fox CS,Liu Y,White CC,Feitosa M,Smith AV,Heard-Costa N,Lohman K,,,,Johnson AD,Foster MC,Greenawalt DM,Griffin P,Ding J,Newman AB,Tylavsky F,Miljkovic I,Kritchevsky SB,Launer L,Garcia M,Eiriksdottir G,Carr JJ,Gudnason V,Harris TB,Cupples LA,Borecki IB

Isolation of novel and known genes from a human fetal cochlear cDNA library using subtractive hybridization and differential screening.
Genomics23(1)42-50(1994 Sep)
Robertson NG,Khetarpal U,Gutiérrez-Espeleta GA,Bieber FR,Morton CC

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EEF2KMT cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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