EEF1B2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EEF1B2
Entrez Gene ID	1933
Full Name	eukaryotic translation elongation factor 1 beta 2
Synonyms	EEF1B,EEF1B1,EF1B
General protein information	Preferred Names eukaryotic translation elongation factor 1 beta 2 Names elongation factor 1-beta EF-1-beta eukaryotic translation elongation factor 1 beta 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q33.3
Summary	This gene encodes a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. Alternative splicing results in three transcript variants which differ only in the 5' UTR. [provided by RefSeq, Jul 2008].
Disorder MIM:	600655

mRNA and Protein(s)

mRNA	Protein	Name
NM_001959.4	NP_001950.1	elongation factor 1-beta
NM_021121.3	NP_066944.1	elongation factor 1-beta
NM_001037663.1	NP_001032752.1	elongation factor 1-beta
NM_001959.3	NP_001950.1	elongation factor 1-beta

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	EEF1B2	XP_004942717.1
Magnaporthe oryzae (rice blast fungus)	MGG_04436	XP_003713531.1
Neurospora crassa	NCU06035	XP_959625.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010612	XP_314575.2
Caenorhabditis elegans (roundworm)	eef-1B.1	NP_498737.1
Xenopus tropicalis (tropical clawed frog)	eef1b2	NP_001006877.1
Eremothecium gossypii	AGOS_AFR003C	NP_985550.1
Schizosaccharomyces pombe (fission yeast)	tef5	NP_588303.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010613	XP_558148.1
Homo sapiens (human)	EEF1B2	NP_066944.1
Bos taurus (cattle)	EEF1B2	NP_001014936.1
Saccharomyces cerevisiae (baker's yeast)	EFB1	NP_009398.1
Macaca mulatta (Rhesus monkey)	EEF1B2	XP_002799072.1
Mus musculus (house mouse)	Eef1b2	NP_061266.2
Drosophila melanogaster (fruit fly)	Ef1beta	NP_524808.3
Arabidopsis thaliana (thale cress)	AT5G12110	NP_196772.1
Arabidopsis thaliana (thale cress)	AT5G19510	NP_568375.2
Pan troglodytes (chimpanzee)	EEF1B2	XP_003309485.1
Canis lupus familiaris (dog)	EEF1B2	XP_536040.1
Rattus norvegicus (Norway rat)	Eef1b2	NP_001102269.1
Danio rerio (zebrafish)	eef1b2	NP_956243.1
Oryza sativa (rice)	Os07g0662500	NP_001060543.1
Kluyveromyces lactis	KLLA0C18469g	XP_453023.1

Gene Ontology
Bibliography

Related articles in PubMed

The protein-binding N-terminal domain of human translation elongation factor 1Bβ possesses a dynamic α-helical structural organization.
Bondarchuk TV, Lozhko DM, Shalak VF, Fatalska A, Szczepanowski RH, Dadlez M, Negrutskii BS, El'skaya AV
International journal of biological macromolecules126899-907(2019 Apr)

Translation elongation factor eEF1Bα is identified as a novel prognostic marker of gastric cancer.
Jia L, Yang T, Gu X, Zhao W, Tang Q, Wang X, Zhu J, Feng Z
International journal of biological macromolecules126345-351(2019 Apr)

An update on the biophysical character of the human eukaryotic elongation factor 1 beta: Perspectives from interaction with elongation factor 1 gamma.
Achilonu I, Elebo N, Hlabano B, Owen GR, Papathanasopoulos M, Dirr HW
Journal of molecular recognition : JMR31(7)e2708(2018 07)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Characterisation of translation elongation factor eEF1B subunit expression in mammalian cells and tissues and co-localisation with eEF1A2.
Cao Y, Portela M, Janikiewicz J, Doig J, Abbott CM
PloS one9(12)e114117(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EEF1B2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EEF1B2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03601	NM_001037663.1 Latest version!	Homo sapiens eukaryotic translation elongation factor 1 beta 2 (EEF1B2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu03601	NM_001959.4 Latest version!	Homo sapiens eukaryotic translation elongation factor 1 beta 2 (EEF1B2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu03601	NM_021121.3 Latest version!	Homo sapiens eukaryotic translation elongation factor 1 beta 2 (EEF1B2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
View Old Accession Versions >>
OHu03601	NM_001959.3	Homo sapiens eukaryotic translation elongation factor 1 beta 2 (EEF1B2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EEF1B2 ( NM_001959.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001950.1 Homo sapiens eukaryotic translation elongation factor 1 beta 2 (EEF1B2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03601
Clone ID Related Accession (Same CDS sequence)	NM_001959.4 , NM_021121.3 , NM_001037663.1 , NM_001959.3
Accession Version	NM_001959.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571760000000
Organism	Homo sapiens(human)
Product	elongation factor 1-beta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from X60489.1. On Nov 23, 2018 this sequence version replaced NM_001959.3. Transcript Variant: This predominant variant (1) has a distinct 5' UTR. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X60489.1, SRR1163655.11650.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000392222.7/ ENSP00000376056.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGTTTCG GAGACCTGAA AAGCCCTGCC GGCCTCCAGG TGCTCAACGA TTACCTGGCG 
GACAAGAGCT ACATCGAGGG GTATGTGCCA TCACAAGCAG ATGTGGCAGT ATTTGAAGCC 
GTGTCCAGCC CACCGCCTGC CGACTTGTGT CATGCCCTAC GTTGGTATAA TCACATCAAG 
TCTTACGAAA AGGAAAAGGC CAGCCTGCCA GGAGTGAAGA AAGCTTTGGG CAAATATGGT 
CCTGCCGATG TGGAAGACAC TACAGGAAGT GGAGCTACAG ATAGTAAAGA TGATGATGAC 
ATTGACCTCT TTGGATCTGA TGATGAGGAG GAAAGTGAAG AAGCAAAGAG GCTAAGGGAA 
GAACGTCTTG CACAATATGA ATCAAAGAAA GCCAAAAAAC CTGCACTTGT TGCCAAGTCT 
TCCATCTTAC TAGATGTGAA ACCTTGGGAT GATGAGACAG ATATGGCGAA ATTAGAGGAG 
TGCGTCAGAA GCATTCAAGC AGACGGCTTA GTCTGGGGCT CATCTAAACT AGTTCCAGTG 
GGATACGGAA TTAAGAAACT TCAAATACAG TGTGTAGTTG AAGATGATAA AGTTGGAACA 
GATATGCTGG AGGAGCAGAT CACTGCTTTT GAGGACTATG TGCAGTCCAT GGATGTGGCT 
GCTTTCAACA AGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001950.1
CDS	86..763
Translation	MGFGDLKSPAGLQVLNDYLADKSYIEGYVPSQADVAVFEAVSSPPPADLCHALRWYNHIKSYEKEKASLPGVKKALGKYGPADVEDTTGSGATDSKDDDDIDLFGSDDEEESEEAKRLREERLAQYESKKAKKPALVAKSSILLDVKPWDDETDMAKLEECVRSIQADGLVWGSSKLVPVGYGIKKLQIQCVVEDDKVGTDMLEEQITAFEDYVQSMDVAAFNKI

Target ORF information:

RefSeq Version	NM_001959.4
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation elongation factor 1 beta 2 (EEF1B2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001959.4

ORF Insert Sequence:

ATGGGTTTCG GAGACCTGAA AAGCCCTGCC GGCCTCCAGG TGCTCAACGA TTACCTGGCG 
GACAAGAGCT ACATCGAGGG GTATGTGCCA TCACAAGCAG ATGTGGCAGT ATTTGAAGCC 
GTGTCCAGCC CACCGCCTGC CGACTTGTGT CATGCCCTAC GTTGGTATAA TCACATCAAG 
TCTTACGAAA AGGAAAAGGC CAGCCTGCCA GGAGTGAAGA AAGCTTTGGG CAAATATGGT 
CCTGCCGATG TGGAAGACAC TACAGGAAGT GGAGCTACAG ATAGTAAAGA TGATGATGAC 
ATTGACCTCT TTGGATCTGA TGATGAGGAG GAAAGTGAAG AAGCAAAGAG GCTAAGGGAA 
GAACGTCTTG CACAATATGA ATCAAAGAAA GCCAAAAAAC CTGCACTTGT TGCCAAGTCT 
TCCATCTTAC TAGATGTGAA ACCTTGGGAT GATGAGACAG ATATGGCGAA ATTAGAGGAG 
TGCGTCAGAA GCATTCAAGC AGACGGCTTA GTCTGGGGCT CATCTAAACT AGTTCCAGTG 
GGATACGGAA TTAAGAAACT TCAAATACAG TGTGTAGTTG AAGATGATAA AGTTGGAACA 
GATATGCTGG AGGAGCAGAT CACTGCTTTT GAGGACTATG TGCAGTCCAT GGATGTGGCT 
GCTTTCAACA AGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEF1B2 ( NM_021121.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_066944.1 Homo sapiens eukaryotic translation elongation factor 1 beta 2 (EEF1B2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu03601
Clone ID Related Accession (Same CDS sequence)	NM_001959.4 , NM_021121.3 , NM_001037663.1 , NM_001959.3
Accession Version	NM_021121.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573660800000
Organism	Homo sapiens(human)
Product	elongation factor 1-beta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU565415.1, X60656.1 and BU627453.1. On Dec 8, 2005 this sequence version replaced NM_021121.2. Transcript Variant: This variant (2) has a distinct 5' UTR compared to variants 1 and 3. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.245059.1, SRR1163657.315360.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGTTTCG GAGACCTGAA AAGCCCTGCC GGCCTCCAGG TGCTCAACGA TTACCTGGCG 
GACAAGAGCT ACATCGAGGG GTATGTGCCA TCACAAGCAG ATGTGGCAGT ATTTGAAGCC 
GTGTCCAGCC CACCGCCTGC CGACTTGTGT CATGCCCTAC GTTGGTATAA TCACATCAAG 
TCTTACGAAA AGGAAAAGGC CAGCCTGCCA GGAGTGAAGA AAGCTTTGGG CAAATATGGT 
CCTGCCGATG TGGAAGACAC TACAGGAAGT GGAGCTACAG ATAGTAAAGA TGATGATGAC 
ATTGACCTCT TTGGATCTGA TGATGAGGAG GAAAGTGAAG AAGCAAAGAG GCTAAGGGAA 
GAACGTCTTG CACAATATGA ATCAAAGAAA GCCAAAAAAC CTGCACTTGT TGCCAAGTCT 
TCCATCTTAC TAGATGTGAA ACCTTGGGAT GATGAGACAG ATATGGCGAA ATTAGAGGAG 
TGCGTCAGAA GCATTCAAGC AGACGGCTTA GTCTGGGGCT CATCTAAACT AGTTCCAGTG 
GGATACGGAA TTAAGAAACT TCAAATACAG TGTGTAGTTG AAGATGATAA AGTTGGAACA 
GATATGCTGG AGGAGCAGAT CACTGCTTTT GAGGACTATG TGCAGTCCAT GGATGTGGCT 
GCTTTCAACA AGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066944.1
CDS	113..790
Translation	MGFGDLKSPAGLQVLNDYLADKSYIEGYVPSQADVAVFEAVSSPPPADLCHALRWYNHIKSYEKEKASLPGVKKALGKYGPADVEDTTGSGATDSKDDDDIDLFGSDDEEESEEAKRLREERLAQYESKKAKKPALVAKSSILLDVKPWDDETDMAKLEECVRSIQADGLVWGSSKLVPVGYGIKKLQIQCVVEDDKVGTDMLEEQITAFEDYVQSMDVAAFNKI

Target ORF information:

RefSeq Version	NM_021121.3
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation elongation factor 1 beta 2 (EEF1B2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021121.3

ORF Insert Sequence:

ATGGGTTTCG GAGACCTGAA AAGCCCTGCC GGCCTCCAGG TGCTCAACGA TTACCTGGCG 
GACAAGAGCT ACATCGAGGG GTATGTGCCA TCACAAGCAG ATGTGGCAGT ATTTGAAGCC 
GTGTCCAGCC CACCGCCTGC CGACTTGTGT CATGCCCTAC GTTGGTATAA TCACATCAAG 
TCTTACGAAA AGGAAAAGGC CAGCCTGCCA GGAGTGAAGA AAGCTTTGGG CAAATATGGT 
CCTGCCGATG TGGAAGACAC TACAGGAAGT GGAGCTACAG ATAGTAAAGA TGATGATGAC 
ATTGACCTCT TTGGATCTGA TGATGAGGAG GAAAGTGAAG AAGCAAAGAG GCTAAGGGAA 
GAACGTCTTG CACAATATGA ATCAAAGAAA GCCAAAAAAC CTGCACTTGT TGCCAAGTCT 
TCCATCTTAC TAGATGTGAA ACCTTGGGAT GATGAGACAG ATATGGCGAA ATTAGAGGAG 
TGCGTCAGAA GCATTCAAGC AGACGGCTTA GTCTGGGGCT CATCTAAACT AGTTCCAGTG 
GGATACGGAA TTAAGAAACT TCAAATACAG TGTGTAGTTG AAGATGATAA AGTTGGAACA 
GATATGCTGG AGGAGCAGAT CACTGCTTTT GAGGACTATG TGCAGTCCAT GGATGTGGCT 
GCTTTCAACA AGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEF1B2 ( NM_001037663.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001032752.1 Homo sapiens eukaryotic translation elongation factor 1 beta 2 (EEF1B2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu03601
Clone ID Related Accession (Same CDS sequence)	NM_001959.4 , NM_021121.3 , NM_001037663.1 , NM_001959.3
Accession Version	NM_001037663.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573747200000
Organism	Homo sapiens(human)
Product	elongation factor 1-beta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU565415.1, BM747256.1 and BU627453.1. Transcript Variant: This variant (3) has a distinct 5' UTR compared to variants 1 and 2. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.579760.1, SRR1163657.14653.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGTTTCG GAGACCTGAA AAGCCCTGCC GGCCTCCAGG TGCTCAACGA TTACCTGGCG 
GACAAGAGCT ACATCGAGGG GTATGTGCCA TCACAAGCAG ATGTGGCAGT ATTTGAAGCC 
GTGTCCAGCC CACCGCCTGC CGACTTGTGT CATGCCCTAC GTTGGTATAA TCACATCAAG 
TCTTACGAAA AGGAAAAGGC CAGCCTGCCA GGAGTGAAGA AAGCTTTGGG CAAATATGGT 
CCTGCCGATG TGGAAGACAC TACAGGAAGT GGAGCTACAG ATAGTAAAGA TGATGATGAC 
ATTGACCTCT TTGGATCTGA TGATGAGGAG GAAAGTGAAG AAGCAAAGAG GCTAAGGGAA 
GAACGTCTTG CACAATATGA ATCAAAGAAA GCCAAAAAAC CTGCACTTGT TGCCAAGTCT 
TCCATCTTAC TAGATGTGAA ACCTTGGGAT GATGAGACAG ATATGGCGAA ATTAGAGGAG 
TGCGTCAGAA GCATTCAAGC AGACGGCTTA GTCTGGGGCT CATCTAAACT AGTTCCAGTG 
GGATACGGAA TTAAGAAACT TCAAATACAG TGTGTAGTTG AAGATGATAA AGTTGGAACA 
GATATGCTGG AGGAGCAGAT CACTGCTTTT GAGGACTATG TGCAGTCCAT GGATGTGGCT 
GCTTTCAACA AGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032752.1
CDS	159..836
Translation	MGFGDLKSPAGLQVLNDYLADKSYIEGYVPSQADVAVFEAVSSPPPADLCHALRWYNHIKSYEKEKASLPGVKKALGKYGPADVEDTTGSGATDSKDDDDIDLFGSDDEEESEEAKRLREERLAQYESKKAKKPALVAKSSILLDVKPWDDETDMAKLEECVRSIQADGLVWGSSKLVPVGYGIKKLQIQCVVEDDKVGTDMLEEQITAFEDYVQSMDVAAFNKI

Target ORF information:

RefSeq Version	NM_001037663.1
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation elongation factor 1 beta 2 (EEF1B2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037663.1

ORF Insert Sequence:

ATGGGTTTCG GAGACCTGAA AAGCCCTGCC GGCCTCCAGG TGCTCAACGA TTACCTGGCG 
GACAAGAGCT ACATCGAGGG GTATGTGCCA TCACAAGCAG ATGTGGCAGT ATTTGAAGCC 
GTGTCCAGCC CACCGCCTGC CGACTTGTGT CATGCCCTAC GTTGGTATAA TCACATCAAG 
TCTTACGAAA AGGAAAAGGC CAGCCTGCCA GGAGTGAAGA AAGCTTTGGG CAAATATGGT 
CCTGCCGATG TGGAAGACAC TACAGGAAGT GGAGCTACAG ATAGTAAAGA TGATGATGAC 
ATTGACCTCT TTGGATCTGA TGATGAGGAG GAAAGTGAAG AAGCAAAGAG GCTAAGGGAA 
GAACGTCTTG CACAATATGA ATCAAAGAAA GCCAAAAAAC CTGCACTTGT TGCCAAGTCT 
TCCATCTTAC TAGATGTGAA ACCTTGGGAT GATGAGACAG ATATGGCGAA ATTAGAGGAG 
TGCGTCAGAA GCATTCAAGC AGACGGCTTA GTCTGGGGCT CATCTAAACT AGTTCCAGTG 
GGATACGGAA TTAAGAAACT TCAAATACAG TGTGTAGTTG AAGATGATAA AGTTGGAACA 
GATATGCTGG AGGAGCAGAT CACTGCTTTT GAGGACTATG TGCAGTCCAT GGATGTGGCT 
GCTTTCAACA AGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEF1B2 ( NM_001959.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001950.1 Homo sapiens eukaryotic translation elongation factor 1 beta 2 (EEF1B2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03601
Clone ID Related Accession (Same CDS sequence)	NM_001959.4 , NM_021121.3 , NM_001037663.1 , NM_001959.3
Accession Version	NM_001959.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1514217600000
Organism	Homo sapiens(human)
Product	elongation factor 1-beta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from BF669470.1, X60489.1 and BU627453.1. On Dec 8, 2005 this sequence version replaced NM_001959.2. Transcript Variant: This predominant variant (1) has a distinct 5' UTR. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.645699.1, SRR1163657.424007.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGTTTCG GAGACCTGAA AAGCCCTGCC GGCCTCCAGG TGCTCAACGA TTACCTGGCG 
GACAAGAGCT ACATCGAGGG GTATGTGCCA TCACAAGCAG ATGTGGCAGT ATTTGAAGCC 
GTGTCCAGCC CACCGCCTGC CGACTTGTGT CATGCCCTAC GTTGGTATAA TCACATCAAG 
TCTTACGAAA AGGAAAAGGC CAGCCTGCCA GGAGTGAAGA AAGCTTTGGG CAAATATGGT 
CCTGCCGATG TGGAAGACAC TACAGGAAGT GGAGCTACAG ATAGTAAAGA TGATGATGAC 
ATTGACCTCT TTGGATCTGA TGATGAGGAG GAAAGTGAAG AAGCAAAGAG GCTAAGGGAA 
GAACGTCTTG CACAATATGA ATCAAAGAAA GCCAAAAAAC CTGCACTTGT TGCCAAGTCT 
TCCATCTTAC TAGATGTGAA ACCTTGGGAT GATGAGACAG ATATGGCGAA ATTAGAGGAG 
TGCGTCAGAA GCATTCAAGC AGACGGCTTA GTCTGGGGCT CATCTAAACT AGTTCCAGTG 
GGATACGGAA TTAAGAAACT TCAAATACAG TGTGTAGTTG AAGATGATAA AGTTGGAACA 
GATATGCTGG AGGAGCAGAT CACTGCTTTT GAGGACTATG TGCAGTCCAT GGATGTGGCT 
GCTTTCAACA AGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001950.1
CDS	387..1064
Translation	MGFGDLKSPAGLQVLNDYLADKSYIEGYVPSQADVAVFEAVSSPPPADLCHALRWYNHIKSYEKEKASLPGVKKALGKYGPADVEDTTGSGATDSKDDDDIDLFGSDDEEESEEAKRLREERLAQYESKKAKKPALVAKSSILLDVKPWDDETDMAKLEECVRSIQADGLVWGSSKLVPVGYGIKKLQIQCVVEDDKVGTDMLEEQITAFEDYVQSMDVAAFNKI

Target ORF information:

RefSeq Version	NM_001959.3
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation elongation factor 1 beta 2 (EEF1B2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001959.3

ORF Insert Sequence:

ATGGGTTTCG GAGACCTGAA AAGCCCTGCC GGCCTCCAGG TGCTCAACGA TTACCTGGCG 
GACAAGAGCT ACATCGAGGG GTATGTGCCA TCACAAGCAG ATGTGGCAGT ATTTGAAGCC 
GTGTCCAGCC CACCGCCTGC CGACTTGTGT CATGCCCTAC GTTGGTATAA TCACATCAAG 
TCTTACGAAA AGGAAAAGGC CAGCCTGCCA GGAGTGAAGA AAGCTTTGGG CAAATATGGT 
CCTGCCGATG TGGAAGACAC TACAGGAAGT GGAGCTACAG ATAGTAAAGA TGATGATGAC 
ATTGACCTCT TTGGATCTGA TGATGAGGAG GAAAGTGAAG AAGCAAAGAG GCTAAGGGAA 
GAACGTCTTG CACAATATGA ATCAAAGAAA GCCAAAAAAC CTGCACTTGT TGCCAAGTCT 
TCCATCTTAC TAGATGTGAA ACCTTGGGAT GATGAGACAG ATATGGCGAA ATTAGAGGAG 
TGCGTCAGAA GCATTCAAGC AGACGGCTTA GTCTGGGGCT CATCTAAACT AGTTCCAGTG 
GGATACGGAA TTAAGAAACT TCAAATACAG TGTGTAGTTG AAGATGATAA AGTTGGAACA 
GATATGCTGG AGGAGCAGAT CACTGCTTTT GAGGACTATG TGCAGTCCAT GGATGTGGCT 
GCTTTCAACA AGATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The protein-binding N-terminal domain of human translation elongation factor 1Bβ possesses a dynamic α-helical structural organization.
International journal of biological macromolecules126899-907(2019 Apr)
Bondarchuk TV,Lozhko DM,Shalak VF,Fatalska A,Szczepanowski RH,Dadlez M,Negrutskii BS,El'skaya AV

Translation elongation factor eEF1Bα is identified as a novel prognostic marker of gastric cancer.
International journal of biological macromolecules126345-351(2019 Apr)
Jia L,Yang T,Gu X,Zhao W,Tang Q,Wang X,Zhu J,Feng Z

An update on the biophysical character of the human eukaryotic elongation factor 1 beta: Perspectives from interaction with elongation factor 1 gamma.
Journal of molecular recognition : JMR31(7)e2708(2018 07)
Achilonu I,Elebo N,Hlabano B,Owen GR,Papathanasopoulos M,Dirr HW

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Characterisation of translation elongation factor eEF1B subunit expression in mammalian cells and tissues and co-localisation with eEF1A2.
PloS one9(12)e114117(2014)
Cao Y,Portela M,Janikiewicz J,Doig J,Abbott CM

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EEF1B2 cDNA ORF clone, Homo sapiens(human)

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