AKAP10 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AKAP10
Entrez Gene ID	11216
Full Name	A-kinase anchoring protein 10
Synonyms	AKAP-10,D-AKAP-2,D-AKAP2,PRKA10
General protein information	Preferred Names A-kinase anchoring protein 10 Names A-kinase anchor protein 10, mitochondrial A kinase (PRKA) anchor protein 10 A kinase anchor protein 10 dual specificity A kinase-anchoring protein 2 mitochondrial A kinase PPKA anchor protein 10 protein kinase A anchoring protein 10
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17p11.2
Summary	This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins bind to the regulatory subunits of protein kinase A (PKA) and confine the holoenzyme to discrete locations within the cell. The encoded protein is localized to mitochondria and interacts with both the type I and type II regulatory subunits of PKA. Polymorphisms in this gene may be associated with increased risk of arrhythmias and sudden cardiac death. [provided by RefSeq, May 2012].
Disorder MIM:	604694
Disorder Html:	{Cardiac conduction defect, susceptibility to}, 115080 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_007202.4	NP_009133.2	A-kinase anchor protein 10, mitochondrial isoform 1 precursor
NM_001330152.1	NP_001317081.1	A-kinase anchor protein 10, mitochondrial isoform 2
NM_007202.3	NP_009133.2	A-kinase anchor protein 10, mitochondrial isoform 1 precursor
XM_017024071.1	XP_016879560.1	A-kinase anchor protein 10, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007895	XP_317593.4
Homo sapiens (human)	AKAP10	NP_009133.2
Gallus gallus (chicken)	AKAP10	XP_415856.3
Mus musculus (house mouse)	Akap10	NP_064305.2
Bos taurus (cattle)	AKAP10	NP_001091493.1
Rattus norvegicus (Norway rat)	Akap10	NP_001108078.1
Drosophila melanogaster (fruit fly)	pkaap	NP_609783.1
Xenopus tropicalis (tropical clawed frog)	akap10	XP_002938286.2
Pan troglodytes (chimpanzee)	AKAP10	XP_003315625.1
Macaca mulatta (Rhesus monkey)	AKAP10	XP_001099682.2
Canis lupus familiaris (dog)	AKAP10	XP_851367.1
Danio rerio (zebrafish)	akap10	XP_005165633.1

Gene Ontology
Bibliography

Related articles in PubMed

Erythropoietin signaling regulates heme biosynthesis.
Chung J, Wittig JG, Ghamari A, Maeda M, Dailey TA, Bergonia H, Kafina MD, Coughlin EE, Minogue CE, Hebert AS, Li L, Kaplan J, Lodish HF, Bauer DE, Orkin SH, Cantor AB, Maeda T, Phillips JD, Coon JJ, Pagliarini DJ, Dailey HA, Paw BH
eLife6(2017 05)

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

Genetic association of AKAP10 gene polymorphism with reduced risk of preterm birth.
Langmia IM, Apalasamy YD, Suki SZ, Omar SZ, Mohamed Z
Journal of perinatology : official journal of the California Perinatal Association35(9)700-4(2015 Sep)

PKA RIα/A-kinase anchoring proteins 10 signaling pathway and the prognosis of colorectal cancer.
Wang M, Li Y, Wang R, Wang Z, Chen K, Zhou B, Zhou Z, Sun X
Journal of gastroenterology and hepatology30(3)496-503(2015 Mar)

D-AKAP2:PKA RII:PDZK1 ternary complex structure: insights from the nucleation of a polyvalent scaffold.
Sarma GN, Moody IS, Ilouz R, Phan RH, Sankaran B, Hall RA, Taylor SS
Protein science : a publication of the Protein Society24(1)105-16(2015 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AKAP10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AKAP10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu49689	NM_001330152.1 Latest version!	Homo sapiens A-kinase anchoring protein 10 (AKAP10), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu86509	XM_017024071.1 Latest version!	Homo sapiens A-kinase anchoring protein 10 (AKAP10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu21114	NM_007202.4 Latest version!	Homo sapiens A-kinase anchoring protein 10 (AKAP10), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
View Old Accession Versions >>
OHu21114	NM_007202.3	Homo sapiens A-kinase anchoring protein 10 (AKAP10), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AKAP10 ( NM_001330152.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317081.1 Homo sapiens A-kinase anchoring protein 10 (AKAP10), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu49689
Clone ID Related Accession (Same CDS sequence)	NM_001330152.1
Accession Version	NM_001330152.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569168000000
Organism	Homo sapiens(human)
Product	A-kinase anchor protein 10, mitochondrial isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005730.1 and CB242966.1. On Aug 18, 2016 this sequence version replaced XM_006721432.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.195945.1, SRR1660805.23324.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGAGGGGAG CCGGGCCCTC CCCGCGCCAG TCCCCCCGCA CCCTCCGTCC CGACCCGGGC 
CCCGCCATGT CCTTCTTCCG GCGGAAAGTG AAAGGCAAAG AACAAGAGAA GACCTCAGAT 
GTGAAGTCCA TTAAAGCTTC AATATCCGTA CATTCCCCAC AAAAAAGCAC TAAAAATCAT 
GCCTTGCTGG AGGCTGCAGG ACCAAGTCAT GTTGCAATCA ATGCCATTTC TGCCAACATG 
GACTCCTTTT CAAGTAGCAG GACAGCCACA CTTAAGAAGC AGCCAAGCCA CATGGAGGCC 
GCTCATTTTG GTGACCTGGG CAGATCTTGT CTGGACTACC AGACTCAAGA GACCAAATCA 
AGCCTTTCTA AGACCCTTGA ACAAGTCTTG CACGACACTA TTGTCCTCCC TTACTTCATT 
CAATTCATGG AACTTCGGCG AATGGAGCAT TTGGTGAAAT TTTGGTTAGA GGCTGAAAGT 
TTTCATTCAA CAACTTGGTC GCGAATAAGA GCACACAGTC TAAACACAGT GAAGCAGAGC 
TCACTGGCTG AGCCTGTCTC TCCATCTAAA AAGCATGAAA CTACAGCGTC TTTTTTAACT 
GATTCTCTTG ATAAGAGATT GGAGGATTCT GGCTCAGCAC AGTTGTTTAT GACTCATTCA 
GAAGGAATTG ACCTGAATAA TAGAACTAAC AGCACTCAGA ATCACTTGCT GCTTTCCCAG 
GAATGTGACA GTGCCCATTC TCTCCGTCTT GAAATGGCCA GAGCAGGAAC TCACCAAGTT 
TCCATGGAAA CCCAAGAATC TTCCTCTACA CTTACAGTAG CCAGTAGAAA TAGTCCCGCT 
TCTCCACTAA AAGAATTGTC AGGAAAACTA ATGAAAAGTA TAGAACAAGA TGCAGTGAAT 
ACTTTTACCA AATATATATC TCCAGATGCT GCTAAACCAA TACCAATTAC AGAAGCAATG 
AGAAATGACA TCATAGCAAG GATTTGTGGA GAAGATGGAC AGGTGGATCC CAACTGTTTC 
GTTTTGGCAC AGTCCATAGT CTTTAGTGCA ATGGAGCAAG AGCACTTTAG TGAGTTTCTG 
CGAAGTCACC ATTTCTGTAA ATACCAGATT GAAGTGCTGA CCAGTGGAAC TGTTTACCTG 
GCTGACATTC TCTTCTGTGA GTCAGCCCTC TTTTATTTCT CTGAGTACAT GGAAAAAGAG 
GATGCAGTGA ATATCTTACA ATTCTGGTTG GCAGCAGATA ACTTCCAGTC TCAGCTTGCT 
GCCAAAAAGG GCCAATATGA TGGACAGGAG GCACAGAATG ATGCCATGAT TTTATATGAC 
AAGTACTTCT CCCTCCAAGC CACACATCCT CTTGGATTTG ATGATGTTGT ACGATTAGAA 
ATTGAATCCA ATATCTGCAG GGAAGGTGGG CCACTCCCCA ACTGTTTCAC AACTCCATTA 
CGTCAGGCCT GGACAACCAT GGAGAAGTCC AGTGTGAAAA AAGCCAGTAT TAAAATACTG 
AAAAATTTTG ATGAAGCGAT AATTGTGGAT GCGGCAAGTC TGGATCCAGA ATCTTTATAT 
CAACGGACAT ATGCCGGGAA GATGACATTT GGAAGAGTCA GTGACTTGGG GCAATTCATC 
CGAGAATCTG AGCCTGAACC TGATGTAAGG AAATCAAAAG GATCCATGTT CTCACAAGCT 
ATGAAGAAAT GGGTGCAAGG AAATACTGAT GAGGCCCAGG AAGAGCTAGC TTGGAAGATT 
GCTAAAATGA TAGTCAGTGA CATTATGCAG CAGGCTCAGT ATGATCAACC GTTAGAGAAA 
TCTACAAAGT TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317081.1
CDS	178..1992
Translation	MRGAGPSPRQSPRTLRPDPGPAMSFFRRKVKGKEQEKTSDVKSIKASISVHSPQKSTKNHALLEAAGPSHVAINAISANMDSFSSSRTATLKKQPSHMEAAHFGDLGRSCLDYQTQETKSSLSKTLEQVLHDTIVLPYFIQFMELRRMEHLVKFWLEAESFHSTTWSRIRAHSLNTVKQSSLAEPVSPSKKHETTASFLTDSLDKRLEDSGSAQLFMTHSEGIDLNNRTNSTQNHLLLSQECDSAHSLRLEMARAGTHQVSMETQESSSTLTVASRNSPASPLKELSGKLMKSIEQDAVNTFTKYISPDAAKPIPITEAMRNDIIARICGEDGQVDPNCFVLAQSIVFSAMEQEHFSEFLRSHHFCKYQIEVLTSGTVYLADILFCESALFYFSEYMEKEDAVNILQFWLAADNFQSQLAAKKGQYDGQEAQNDAMILYDKYFSLQATHPLGFDDVVRLEIESNICREGGPLPNCFTTPLRQAWTTMEKSSVKKASIKILKNFDEAIIVDAASLDPESLYQRTYAGKMTFGRVSDLGQFIRESEPEPDVRKSKGSMFSQAMKKWVQGNTDEAQEELAWKIAKMIVSDIMQQAQYDQPLEKSTKL

Target ORF information:

RefSeq Version	NM_001330152.1
Organism	Homo sapiens(human)
Definition	Homo sapiens A-kinase anchoring protein 10 (AKAP10), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330152.1

ORF Insert Sequence:

ATGAGGGGAG CCGGGCCCTC CCCGCGCCAG TCCCCCCGCA CCCTCCGTCC CGACCCGGGC 
CCCGCCATGT CCTTCTTCCG GCGGAAAGTG AAAGGCAAAG AACAAGAGAA GACCTCAGAT 
GTGAAGTCCA TTAAAGCTTC AATATCCGTA CATTCCCCAC AAAAAAGCAC TAAAAATCAT 
GCCTTGCTGG AGGCTGCAGG ACCAAGTCAT GTTGCAATCA ATGCCATTTC TGCCAACATG 
GACTCCTTTT CAAGTAGCAG GACAGCCACA CTTAAGAAGC AGCCAAGCCA CATGGAGGCC 
GCTCATTTTG GTGACCTGGG CAGATCTTGT CTGGACTACC AGACTCAAGA GACCAAATCA 
AGCCTTTCTA AGACCCTTGA ACAAGTCTTG CACGACACTA TTGTCCTCCC TTACTTCATT 
CAATTCATGG AACTTCGGCG AATGGAGCAT TTGGTGAAAT TTTGGTTAGA GGCTGAAAGT 
TTTCATTCAA CAACTTGGTC GCGAATAAGA GCACACAGTC TAAACACAGT GAAGCAGAGC 
TCACTGGCTG AGCCTGTCTC TCCATCTAAA AAGCATGAAA CTACAGCGTC TTTTTTAACT 
GATTCTCTTG ATAAGAGATT GGAGGATTCT GGCTCAGCAC AGTTGTTTAT GACTCATTCA 
GAAGGAATTG ACCTGAATAA TAGAACTAAC AGCACTCAGA ATCACTTGCT GCTTTCCCAG 
GAATGTGACA GTGCCCATTC TCTCCGTCTT GAAATGGCCA GAGCAGGAAC TCACCAAGTT 
TCCATGGAAA CCCAAGAATC TTCCTCTACA CTTACAGTAG CCAGTAGAAA TAGTCCCGCT 
TCTCCACTAA AAGAATTGTC AGGAAAACTA ATGAAAAGTA TAGAACAAGA TGCAGTGAAT 
ACTTTTACCA AATATATATC TCCAGATGCT GCTAAACCAA TACCAATTAC AGAAGCAATG 
AGAAATGACA TCATAGCAAG GATTTGTGGA GAAGATGGAC AGGTGGATCC CAACTGTTTC 
GTTTTGGCAC AGTCCATAGT CTTTAGTGCA ATGGAGCAAG AGCACTTTAG TGAGTTTCTG 
CGAAGTCACC ATTTCTGTAA ATACCAGATT GAAGTGCTGA CCAGTGGAAC TGTTTACCTG 
GCTGACATTC TCTTCTGTGA GTCAGCCCTC TTTTATTTCT CTGAGTACAT GGAAAAAGAG 
GATGCAGTGA ATATCTTACA ATTCTGGTTG GCAGCAGATA ACTTCCAGTC TCAGCTTGCT 
GCCAAAAAGG GCCAATATGA TGGACAGGAG GCACAGAATG ATGCCATGAT TTTATATGAC 
AAGTACTTCT CCCTCCAAGC CACACATCCT CTTGGATTTG ATGATGTTGT ACGATTAGAA 
ATTGAATCCA ATATCTGCAG GGAAGGTGGG CCACTCCCCA ACTGTTTCAC AACTCCATTA 
CGTCAGGCCT GGACAACCAT GGAGAAGTCC AGTGTGAAAA AAGCCAGTAT TAAAATACTG 
AAAAATTTTG ATGAAGCGAT AATTGTGGAT GCGGCAAGTC TGGATCCAGA ATCTTTATAT 
CAACGGACAT ATGCCGGGAA GATGACATTT GGAAGAGTCA GTGACTTGGG GCAATTCATC 
CGAGAATCTG AGCCTGAACC TGATGTAAGG AAATCAAAAG GATCCATGTT CTCACAAGCT 
ATGAAGAAAT GGGTGCAAGG AAATACTGAT GAGGCCCAGG AAGAGCTAGC TTGGAAGATT 
GCTAAAATGA TAGTCAGTGA CATTATGCAG CAGGCTCAGT ATGATCAACC GTTAGAGAAA 
TCTACAAAGT TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKAP10 ( XM_017024071.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879560.1 Homo sapiens A-kinase anchoring protein 10 (AKAP10), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu86509
Clone ID Related Accession (Same CDS sequence)	XM_017024071.1
Accession Version	XM_017024071.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	A-kinase anchor protein 10, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGGAG CCGGGCCCTC CCCGCGCCAG TCCCCCCGCA CCCTCCGTCC CGACCCGGGC 
CCCGCCATGT CCTTCTTCCG GCGGAAAGTG AAAGGCAAAG AACAAGAGAA GACCTCAGAT 
GTGAAGTCCA TTAAAGCTTC AATATCCGTA CATTCCCCAC AAAAAAGCAC TAAAAATCAT 
GCCTTGCTGG AGGCTGCAGG ACCAAGTCAT GTTGCAATCA ATGCCATTTC TGCCAACATG 
GACTCCTTTT CAAGTAGCAG GACAGCCACA CTTAAGAAGC AGCCAAGCCA CATGGAGGCC 
GCTCATTTTG GTGACCTGGG CAGATCTTGT CTGGACTACC AGACTCAAGA GACCAAATCA 
AGCCTTTCTA AGACCCTTGA ACAAGTCTTG CACGACACTA TTGTCCTCCC TTACTTCATT 
CAATTCATGG AACTTCGGCG AATGGAGCAT TTGGTGAAAT TTTGGTTAGA GGCTGAAAGT 
TTTCATTCAA CAACTTGGTC GCGAATAAGA GCACACAGTC TAAACACAGT GAAGCAGAGC 
TCACTGGCTG AGCCTGTCTC TCCATCTAAA AAGCATGAAA CTACAGCGTC TTTTTTAACT 
GATTCTCTTG ATAAGAGATT GGAGGATTCT GGCTCAGCAC AGTTGTTTAT GACTCATTCA 
GAAGGAATTG ACCTGAATAA TAGAACTAAC AGCACTCAGA ATCACTTGCT GCTTTCCCAG 
GAATGTGACA GTGCCCATTC TCTCCGTCTT GAAATGGCCA GAGCAGGAAC TCACCAAGTT 
TCCATGGAAA CCCAAGAATC TTCCTCTACA CTTACAGTAG CCAGTAGAAA TAGTCCCGCT 
TCTCCACTAA AAGAATTGTC AGGAAAACTA ATGAAAAGTA TAGAACAAGA TGCAGTGAAT 
ACTTTTACCA AATATATATC TCCAGATGCT GCTAAACCAA TACCAATTAC AGAAGCAATG 
AGAAATGACA TCATAGCAAG GATTTGTGGA GAAGATGGAC AGGTGGATCC CAACTGTTTC 
GTTTTGGCAC AGTCCATAGT CTTTAGTGCA ATGGAGCAAG AGCACTTTAG TGAGTTTCTG 
CGAAGTCACC ATTTCTGTAA ATACCAGATT GAAGTGCTGA CCAGTGGAAC TGTTTACCTG 
GCTGACATTC TCTTCTGTGA GTCAGCCCTC TTTTATTTCT CTGAGTACAT GGAAAAAGAG 
GATGCAGTGA ATATCTTACA ATTCTGGTTG GCAGCAGATA ACTTCCAGTC TCAGCTTGCT 
GCCAAAAAGG GCCAATATGA TGGACAGGAG GCACAGAATG ATGCCATGAT TTTATATGAC 
AAGTACTTCT CCCTCCAAGC CACACATCCT CTTGGATTTG ATGATGTTGT ACGATTAGAA 
ATTGAATCCA ATATCTGCAG GGAAGGTGGG CCACTCCCCA ACTGTTTCAC AACTCCATTA 
CGTCAGGCCT GGACAACCAT GGAGAAGGTC TTTTTGCCTG GCTTTTTGTC CAGCAATCTT 
TATTATAAAT ATTTGAATGA TCTCATCCAT TCGGTTCGAG GAGATGAATT TCTGGGCGGG 
AACGTGTCGC TGACTGCTCC TGGCTCTGTT GGCCCTCCTG ATGAGTCTCA CCCAGGGAGT 
TCTGACAGCT CTGCGTCTCA GTCCAGTGTG AAAAAAGCCA GTATTAAAAT ACTGAAAAAT 
TTTGATGAAG CGATAATTGT GGATGCGGCA AGTCTGGATC CAGAATCTTT ATATCAACGG 
ACATATGCCG GGAAGATGAC ATTTGGAAGA GTCAGTGACT TGGGGCAATT CATCCGAGAA 
TCTGAGCCTG AACCTGATGT AAGGAAATCA AAAGTTCTTG CTCTGTCACC CAGACCGGAG 
TGCAGTGGTC TGACCGCACC TCACTGCAAC CTCTGCTTCC TAGGTTCAAG CGATTTTCCT 
GCCTCATCCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879560.1
CDS	173..2107
Translation	MRGAGPSPRQSPRTLRPDPGPAMSFFRRKVKGKEQEKTSDVKSIKASISVHSPQKSTKNHALLEAAGPSHVAINAISANMDSFSSSRTATLKKQPSHMEAAHFGDLGRSCLDYQTQETKSSLSKTLEQVLHDTIVLPYFIQFMELRRMEHLVKFWLEAESFHSTTWSRIRAHSLNTVKQSSLAEPVSPSKKHETTASFLTDSLDKRLEDSGSAQLFMTHSEGIDLNNRTNSTQNHLLLSQECDSAHSLRLEMARAGTHQVSMETQESSSTLTVASRNSPASPLKELSGKLMKSIEQDAVNTFTKYISPDAAKPIPITEAMRNDIIARICGEDGQVDPNCFVLAQSIVFSAMEQEHFSEFLRSHHFCKYQIEVLTSGTVYLADILFCESALFYFSEYMEKEDAVNILQFWLAADNFQSQLAAKKGQYDGQEAQNDAMILYDKYFSLQATHPLGFDDVVRLEIESNICREGGPLPNCFTTPLRQAWTTMEKVFLPGFLSSNLYYKYLNDLIHSVRGDEFLGGNVSLTAPGSVGPPDESHPGSSDSSASQSSVKKASIKILKNFDEAIIVDAASLDPESLYQRTYAGKMTFGRVSDLGQFIRESEPEPDVRKSKVLALSPRPECSGLTAPHCNLCFLGSSDFPASSS

Target ORF information:

RefSeq Version	XM_017024071.1
Organism	Homo sapiens(human)
Definition	Homo sapiens A-kinase anchoring protein 10 (AKAP10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024071.1

ORF Insert Sequence:

ATGAGGGGAG CCGGGCCCTC CCCGCGCCAG TCCCCCCGCA CCCTCCGTCC CGACCCGGGC 
CCCGCCATGT CCTTCTTCCG GCGGAAAGTG AAAGGCAAAG AACAAGAGAA GACCTCAGAT 
GTGAAGTCCA TTAAAGCTTC AATATCCGTA CATTCCCCAC AAAAAAGCAC TAAAAATCAT 
GCCTTGCTGG AGGCTGCAGG ACCAAGTCAT GTTGCAATCA ATGCCATTTC TGCCAACATG 
GACTCCTTTT CAAGTAGCAG GACAGCCACA CTTAAGAAGC AGCCAAGCCA CATGGAGGCC 
GCTCATTTTG GTGACCTGGG CAGATCTTGT CTGGACTACC AGACTCAAGA GACCAAATCA 
AGCCTTTCTA AGACCCTTGA ACAAGTCTTG CACGACACTA TTGTCCTCCC TTACTTCATT 
CAATTCATGG AACTTCGGCG AATGGAGCAT TTGGTGAAAT TTTGGTTAGA GGCTGAAAGT 
TTTCATTCAA CAACTTGGTC GCGAATAAGA GCACACAGTC TAAACACAGT GAAGCAGAGC 
TCACTGGCTG AGCCTGTCTC TCCATCTAAA AAGCATGAAA CTACAGCGTC TTTTTTAACT 
GATTCTCTTG ATAAGAGATT GGAGGATTCT GGCTCAGCAC AGTTGTTTAT GACTCATTCA 
GAAGGAATTG ACCTGAATAA TAGAACTAAC AGCACTCAGA ATCACTTGCT GCTTTCCCAG 
GAATGTGACA GTGCCCATTC TCTCCGTCTT GAAATGGCCA GAGCAGGAAC TCACCAAGTT 
TCCATGGAAA CCCAAGAATC TTCCTCTACA CTTACAGTAG CCAGTAGAAA TAGTCCCGCT 
TCTCCACTAA AAGAATTGTC AGGAAAACTA ATGAAAAGTA TAGAACAAGA TGCAGTGAAT 
ACTTTTACCA AATATATATC TCCAGATGCT GCTAAACCAA TACCAATTAC AGAAGCAATG 
AGAAATGACA TCATAGCAAG GATTTGTGGA GAAGATGGAC AGGTGGATCC CAACTGTTTC 
GTTTTGGCAC AGTCCATAGT CTTTAGTGCA ATGGAGCAAG AGCACTTTAG TGAGTTTCTG 
CGAAGTCACC ATTTCTGTAA ATACCAGATT GAAGTGCTGA CCAGTGGAAC TGTTTACCTG 
GCTGACATTC TCTTCTGTGA GTCAGCCCTC TTTTATTTCT CTGAGTACAT GGAAAAAGAG 
GATGCAGTGA ATATCTTACA ATTCTGGTTG GCAGCAGATA ACTTCCAGTC TCAGCTTGCT 
GCCAAAAAGG GCCAATATGA TGGACAGGAG GCACAGAATG ATGCCATGAT TTTATATGAC 
AAGTACTTCT CCCTCCAAGC CACACATCCT CTTGGATTTG ATGATGTTGT ACGATTAGAA 
ATTGAATCCA ATATCTGCAG GGAAGGTGGG CCACTCCCCA ACTGTTTCAC AACTCCATTA 
CGTCAGGCCT GGACAACCAT GGAGAAGGTC TTTTTGCCTG GCTTTTTGTC CAGCAATCTT 
TATTATAAAT ATTTGAATGA TCTCATCCAT TCGGTTCGAG GAGATGAATT TCTGGGCGGG 
AACGTGTCGC TGACTGCTCC TGGCTCTGTT GGCCCTCCTG ATGAGTCTCA CCCAGGGAGT 
TCTGACAGCT CTGCGTCTCA GTCCAGTGTG AAAAAAGCCA GTATTAAAAT ACTGAAAAAT 
TTTGATGAAG CGATAATTGT GGATGCGGCA AGTCTGGATC CAGAATCTTT ATATCAACGG 
ACATATGCCG GGAAGATGAC ATTTGGAAGA GTCAGTGACT TGGGGCAATT CATCCGAGAA 
TCTGAGCCTG AACCTGATGT AAGGAAATCA AAAGTTCTTG CTCTGTCACC CAGACCGGAG 
TGCAGTGGTC TGACCGCACC TCACTGCAAC CTCTGCTTCC TAGGTTCAAG CGATTTTCCT 
GCCTCATCCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKAP10 ( NM_007202.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_009133.2 Homo sapiens A-kinase anchoring protein 10 (AKAP10), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21114
Clone ID Related Accession (Same CDS sequence)	NM_007202.4 , NM_007202.3
Accession Version	NM_007202.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577548800000
Organism	Homo sapiens(human)
Product	A-kinase anchor protein 10, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP229845.1, BC017055.1, AC005730.1 and AC015726.8. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_007202.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.256198.1, SRR1803612.244754.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000225737.11/ ENSP00000225737.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGGGGAG CCGGGCCCTC CCCGCGCCAG TCCCCCCGCA CCCTCCGTCC CGACCCGGGC 
CCCGCCATGT CCTTCTTCCG GCGGAAAGTG AAAGGCAAAG AACAAGAGAA GACCTCAGAT 
GTGAAGTCCA TTAAAGCTTC AATATCCGTA CATTCCCCAC AAAAAAGCAC TAAAAATCAT 
GCCTTGCTGG AGGCTGCAGG ACCAAGTCAT GTTGCAATCA ATGCCATTTC TGCCAACATG 
GACTCCTTTT CAAGTAGCAG GACAGCCACA CTTAAGAAGC AGCCAAGCCA CATGGAGGCC 
GCTCATTTTG GTGACCTGGG CAGATCTTGT CTGGACTACC AGACTCAAGA GACCAAATCA 
AGCCTTTCTA AGACCCTTGA ACAAGTCTTG CACGACACTA TTGTCCTCCC TTACTTCATT 
CAATTCATGG AACTTCGGCG AATGGAGCAT TTGGTGAAAT TTTGGTTAGA GGCTGAAAGT 
TTTCATTCAA CAACTTGGTC GCGAATAAGA GCACACAGTC TAAACACAGT GAAGCAGAGC 
TCACTGGCTG AGCCTGTCTC TCCATCTAAA AAGCATGAAA CTACAGCGTC TTTTTTAACT 
GATTCTCTTG ATAAGAGATT GGAGGATTCT GGCTCAGCAC AGTTGTTTAT GACTCATTCA 
GAAGGAATTG ACCTGAATAA TAGAACTAAC AGCACTCAGA ATCACTTGCT GCTTTCCCAG 
GAATGTGACA GTGCCCATTC TCTCCGTCTT GAAATGGCCA GAGCAGGAAC TCACCAAGTT 
TCCATGGAAA CCCAAGAATC TTCCTCTACA CTTACAGTAG CCAGTAGAAA TAGTCCCGCT 
TCTCCACTAA AAGAATTGTC AGGAAAACTA ATGAAAAGTA TAGAACAAGA TGCAGTGAAT 
ACTTTTACCA AATATATATC TCCAGATGCT GCTAAACCAA TACCAATTAC AGAAGCAATG 
AGAAATGACA TCATAGCAAG GATTTGTGGA GAAGATGGAC AGGTGGATCC CAACTGTTTC 
GTTTTGGCAC AGTCCATAGT CTTTAGTGCA ATGGAGCAAG AGCACTTTAG TGAGTTTCTG 
CGAAGTCACC ATTTCTGTAA ATACCAGATT GAAGTGCTGA CCAGTGGAAC TGTTTACCTG 
GCTGACATTC TCTTCTGTGA GTCAGCCCTC TTTTATTTCT CTGAGTACAT GGAAAAAGAG 
GATGCAGTGA ATATCTTACA ATTCTGGTTG GCAGCAGATA ACTTCCAGTC TCAGCTTGCT 
GCCAAAAAGG GCCAATATGA TGGACAGGAG GCACAGAATG ATGCCATGAT TTTATATGAC 
AAGTACTTCT CCCTCCAAGC CACACATCCT CTTGGATTTG ATGATGTTGT ACGATTAGAA 
ATTGAATCCA ATATCTGCAG GGAAGGTGGG CCACTCCCCA ACTGTTTCAC AACTCCATTA 
CGTCAGGCCT GGACAACCAT GGAGAAGGTC TTTTTGCCTG GCTTTTTGTC CAGCAATCTT 
TATTATAAAT ATTTGAATGA TCTCATCCAT TCGGTTCGAG GAGATGAATT TCTGGGCGGG 
AACGTGTCGC TGACTGCTCC TGGCTCTGTT GGCCCTCCTG ATGAGTCTCA CCCAGGGAGT 
TCTGACAGCT CTGCGTCTCA GTCCAGTGTG AAAAAAGCCA GTATTAAAAT ACTGAAAAAT 
TTTGATGAAG CGATAATTGT GGATGCGGCA AGTCTGGATC CAGAATCTTT ATATCAACGG 
ACATATGCCG GGAAGATGAC ATTTGGAAGA GTCAGTGACT TGGGGCAATT CATCCGAGAA 
TCTGAGCCTG AACCTGATGT AAGGAAATCA AAAGGATCCA TGTTCTCACA AGCTATGAAG 
AAATGGGTGC AAGGAAATAC TGATGAGGCC CAGGAAGAGC TAGCTTGGAA GATTGCTAAA 
ATGATAGTCA GTGACATTAT GCAGCAGGCT CAGTATGATC AACCGTTAGA GAAATCTACA 
AAGTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009133.2
CDS	150..2138
Translation	MRGAGPSPRQSPRTLRPDPGPAMSFFRRKVKGKEQEKTSDVKSIKASISVHSPQKSTKNHALLEAAGPSHVAINAISANMDSFSSSRTATLKKQPSHMEAAHFGDLGRSCLDYQTQETKSSLSKTLEQVLHDTIVLPYFIQFMELRRMEHLVKFWLEAESFHSTTWSRIRAHSLNTVKQSSLAEPVSPSKKHETTASFLTDSLDKRLEDSGSAQLFMTHSEGIDLNNRTNSTQNHLLLSQECDSAHSLRLEMARAGTHQVSMETQESSSTLTVASRNSPASPLKELSGKLMKSIEQDAVNTFTKYISPDAAKPIPITEAMRNDIIARICGEDGQVDPNCFVLAQSIVFSAMEQEHFSEFLRSHHFCKYQIEVLTSGTVYLADILFCESALFYFSEYMEKEDAVNILQFWLAADNFQSQLAAKKGQYDGQEAQNDAMILYDKYFSLQATHPLGFDDVVRLEIESNICREGGPLPNCFTTPLRQAWTTMEKVFLPGFLSSNLYYKYLNDLIHSVRGDEFLGGNVSLTAPGSVGPPDESHPGSSDSSASQSSVKKASIKILKNFDEAIIVDAASLDPESLYQRTYAGKMTFGRVSDLGQFIRESEPEPDVRKSKGSMFSQAMKKWVQGNTDEAQEELAWKIAKMIVSDIMQQAQYDQPLEKSTKL

Target ORF information:

RefSeq Version	NM_007202.4
Organism	Homo sapiens(human)
Definition	Homo sapiens A-kinase anchoring protein 10 (AKAP10), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007202.4

ORF Insert Sequence:

ATGAGGGGAG CCGGGCCCTC CCCGCGCCAG TCCCCCCGCA CCCTCCGTCC CGACCCGGGC 
CCCGCCATGT CCTTCTTCCG GCGGAAAGTG AAAGGCAAAG AACAAGAGAA GACCTCAGAT 
GTGAAGTCCA TTAAAGCTTC AATATCCGTA CATTCCCCAC AAAAAAGCAC TAAAAATCAT 
GCCTTGCTGG AGGCTGCAGG ACCAAGTCAT GTTGCAATCA ATGCCATTTC TGCCAACATG 
GACTCCTTTT CAAGTAGCAG GACAGCCACA CTTAAGAAGC AGCCAAGCCA CATGGAGGCC 
GCTCATTTTG GTGACCTGGG CAGATCTTGT CTGGACTACC AGACTCAAGA GACCAAATCA 
AGCCTTTCTA AGACCCTTGA ACAAGTCTTG CACGACACTA TTGTCCTCCC TTACTTCATT 
CAATTCATGG AACTTCGGCG AATGGAGCAT TTGGTGAAAT TTTGGTTAGA GGCTGAAAGT 
TTTCATTCAA CAACTTGGTC GCGAATAAGA GCACACAGTC TAAACACAGT GAAGCAGAGC 
TCACTGGCTG AGCCTGTCTC TCCATCTAAA AAGCATGAAA CTACAGCGTC TTTTTTAACT 
GATTCTCTTG ATAAGAGATT GGAGGATTCT GGCTCAGCAC AGTTGTTTAT GACTCATTCA 
GAAGGAATTG ACCTGAATAA TAGAACTAAC AGCACTCAGA ATCACTTGCT GCTTTCCCAG 
GAATGTGACA GTGCCCATTC TCTCCGTCTT GAAATGGCCA GAGCAGGAAC TCACCAAGTT 
TCCATGGAAA CCCAAGAATC TTCCTCTACA CTTACAGTAG CCAGTAGAAA TAGTCCCGCT 
TCTCCACTAA AAGAATTGTC AGGAAAACTA ATGAAAAGTA TAGAACAAGA TGCAGTGAAT 
ACTTTTACCA AATATATATC TCCAGATGCT GCTAAACCAA TACCAATTAC AGAAGCAATG 
AGAAATGACA TCATAGCAAG GATTTGTGGA GAAGATGGAC AGGTGGATCC CAACTGTTTC 
GTTTTGGCAC AGTCCATAGT CTTTAGTGCA ATGGAGCAAG AGCACTTTAG TGAGTTTCTG 
CGAAGTCACC ATTTCTGTAA ATACCAGATT GAAGTGCTGA CCAGTGGAAC TGTTTACCTG 
GCTGACATTC TCTTCTGTGA GTCAGCCCTC TTTTATTTCT CTGAGTACAT GGAAAAAGAG 
GATGCAGTGA ATATCTTACA ATTCTGGTTG GCAGCAGATA ACTTCCAGTC TCAGCTTGCT 
GCCAAAAAGG GCCAATATGA TGGACAGGAG GCACAGAATG ATGCCATGAT TTTATATGAC 
AAGTACTTCT CCCTCCAAGC CACACATCCT CTTGGATTTG ATGATGTTGT ACGATTAGAA 
ATTGAATCCA ATATCTGCAG GGAAGGTGGG CCACTCCCCA ACTGTTTCAC AACTCCATTA 
CGTCAGGCCT GGACAACCAT GGAGAAGGTC TTTTTGCCTG GCTTTTTGTC CAGCAATCTT 
TATTATAAAT ATTTGAATGA TCTCATCCAT TCGGTTCGAG GAGATGAATT TCTGGGCGGG 
AACGTGTCGC TGACTGCTCC TGGCTCTGTT GGCCCTCCTG ATGAGTCTCA CCCAGGGAGT 
TCTGACAGCT CTGCGTCTCA GTCCAGTGTG AAAAAAGCCA GTATTAAAAT ACTGAAAAAT 
TTTGATGAAG CGATAATTGT GGATGCGGCA AGTCTGGATC CAGAATCTTT ATATCAACGG 
ACATATGCCG GGAAGATGAC ATTTGGAAGA GTCAGTGACT TGGGGCAATT CATCCGAGAA 
TCTGAGCCTG AACCTGATGT AAGGAAATCA AAAGGATCCA TGTTCTCACA AGCTATGAAG 
AAATGGGTGC AAGGAAATAC TGATGAGGCC CAGGAAGAGC TAGCTTGGAA GATTGCTAAA 
ATGATAGTCA GTGACATTAT GCAGCAGGCT CAGTATGATC AACCGTTAGA GAAATCTACA 
AAGTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AKAP10 ( NM_007202.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_009133.2 Homo sapiens A-kinase anchoring protein 10 (AKAP10), transcript variant 1, mRNA.

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CloneID	OHu21114
Clone ID Related Accession (Same CDS sequence)	NM_007202.4 , NM_007202.3
Accession Version	NM_007202.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Homo sapiens(human)
Product	A-kinase anchor protein 10, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP229845.1, BC017055.1, AC005730.1 and AC015726.8. This sequence is a reference standard in the RefSeqGene project. On May 11, 2012 this sequence version replaced NM_007202.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.244754.1, SRR1803611.140541.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGGGGAG CCGGGCCCTC CCCGCGCCAG TCCCCCCGCA CCCTCCGTCC CGACCCGGGC 
CCCGCCATGT CCTTCTTCCG GCGGAAAGTG AAAGGCAAAG AACAAGAGAA GACCTCAGAT 
GTGAAGTCCA TTAAAGCTTC AATATCCGTA CATTCCCCAC AAAAAAGCAC TAAAAATCAT 
GCCTTGCTGG AGGCTGCAGG ACCAAGTCAT GTTGCAATCA ATGCCATTTC TGCCAACATG 
GACTCCTTTT CAAGTAGCAG GACAGCCACA CTTAAGAAGC AGCCAAGCCA CATGGAGGCC 
GCTCATTTTG GTGACCTGGG CAGATCTTGT CTGGACTACC AGACTCAAGA GACCAAATCA 
AGCCTTTCTA AGACCCTTGA ACAAGTCTTG CACGACACTA TTGTCCTCCC TTACTTCATT 
CAATTCATGG AACTTCGGCG AATGGAGCAT TTGGTGAAAT TTTGGTTAGA GGCTGAAAGT 
TTTCATTCAA CAACTTGGTC GCGAATAAGA GCACACAGTC TAAACACAGT GAAGCAGAGC 
TCACTGGCTG AGCCTGTCTC TCCATCTAAA AAGCATGAAA CTACAGCGTC TTTTTTAACT 
GATTCTCTTG ATAAGAGATT GGAGGATTCT GGCTCAGCAC AGTTGTTTAT GACTCATTCA 
GAAGGAATTG ACCTGAATAA TAGAACTAAC AGCACTCAGA ATCACTTGCT GCTTTCCCAG 
GAATGTGACA GTGCCCATTC TCTCCGTCTT GAAATGGCCA GAGCAGGAAC TCACCAAGTT 
TCCATGGAAA CCCAAGAATC TTCCTCTACA CTTACAGTAG CCAGTAGAAA TAGTCCCGCT 
TCTCCACTAA AAGAATTGTC AGGAAAACTA ATGAAAAGTA TAGAACAAGA TGCAGTGAAT 
ACTTTTACCA AATATATATC TCCAGATGCT GCTAAACCAA TACCAATTAC AGAAGCAATG 
AGAAATGACA TCATAGCAAG GATTTGTGGA GAAGATGGAC AGGTGGATCC CAACTGTTTC 
GTTTTGGCAC AGTCCATAGT CTTTAGTGCA ATGGAGCAAG AGCACTTTAG TGAGTTTCTG 
CGAAGTCACC ATTTCTGTAA ATACCAGATT GAAGTGCTGA CCAGTGGAAC TGTTTACCTG 
GCTGACATTC TCTTCTGTGA GTCAGCCCTC TTTTATTTCT CTGAGTACAT GGAAAAAGAG 
GATGCAGTGA ATATCTTACA ATTCTGGTTG GCAGCAGATA ACTTCCAGTC TCAGCTTGCT 
GCCAAAAAGG GCCAATATGA TGGACAGGAG GCACAGAATG ATGCCATGAT TTTATATGAC 
AAGTACTTCT CCCTCCAAGC CACACATCCT CTTGGATTTG ATGATGTTGT ACGATTAGAA 
ATTGAATCCA ATATCTGCAG GGAAGGTGGG CCACTCCCCA ACTGTTTCAC AACTCCATTA 
CGTCAGGCCT GGACAACCAT GGAGAAGGTC TTTTTGCCTG GCTTTTTGTC CAGCAATCTT 
TATTATAAAT ATTTGAATGA TCTCATCCAT TCGGTTCGAG GAGATGAATT TCTGGGCGGG 
AACGTGTCGC TGACTGCTCC TGGCTCTGTT GGCCCTCCTG ATGAGTCTCA CCCAGGGAGT 
TCTGACAGCT CTGCGTCTCA GTCCAGTGTG AAAAAAGCCA GTATTAAAAT ACTGAAAAAT 
TTTGATGAAG CGATAATTGT GGATGCGGCA AGTCTGGATC CAGAATCTTT ATATCAACGG 
ACATATGCCG GGAAGATGAC ATTTGGAAGA GTCAGTGACT TGGGGCAATT CATCCGAGAA 
TCTGAGCCTG AACCTGATGT AAGGAAATCA AAAGGATCCA TGTTCTCACA AGCTATGAAG 
AAATGGGTGC AAGGAAATAC TGATGAGGCC CAGGAAGAGC TAGCTTGGAA GATTGCTAAA 
ATGATAGTCA GTGACATTAT GCAGCAGGCT CAGTATGATC AACCGTTAGA GAAATCTACA 
AAGTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009133.2
CDS	178..2166
Translation	MRGAGPSPRQSPRTLRPDPGPAMSFFRRKVKGKEQEKTSDVKSIKASISVHSPQKSTKNHALLEAAGPSHVAINAISANMDSFSSSRTATLKKQPSHMEAAHFGDLGRSCLDYQTQETKSSLSKTLEQVLHDTIVLPYFIQFMELRRMEHLVKFWLEAESFHSTTWSRIRAHSLNTVKQSSLAEPVSPSKKHETTASFLTDSLDKRLEDSGSAQLFMTHSEGIDLNNRTNSTQNHLLLSQECDSAHSLRLEMARAGTHQVSMETQESSSTLTVASRNSPASPLKELSGKLMKSIEQDAVNTFTKYISPDAAKPIPITEAMRNDIIARICGEDGQVDPNCFVLAQSIVFSAMEQEHFSEFLRSHHFCKYQIEVLTSGTVYLADILFCESALFYFSEYMEKEDAVNILQFWLAADNFQSQLAAKKGQYDGQEAQNDAMILYDKYFSLQATHPLGFDDVVRLEIESNICREGGPLPNCFTTPLRQAWTTMEKVFLPGFLSSNLYYKYLNDLIHSVRGDEFLGGNVSLTAPGSVGPPDESHPGSSDSSASQSSVKKASIKILKNFDEAIIVDAASLDPESLYQRTYAGKMTFGRVSDLGQFIRESEPEPDVRKSKGSMFSQAMKKWVQGNTDEAQEELAWKIAKMIVSDIMQQAQYDQPLEKSTKL

Target ORF information:

RefSeq Version	NM_007202.3
Organism	Homo sapiens(human)
Definition	Homo sapiens A-kinase anchoring protein 10 (AKAP10), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007202.3

ORF Insert Sequence:

ATGAGGGGAG CCGGGCCCTC CCCGCGCCAG TCCCCCCGCA CCCTCCGTCC CGACCCGGGC 
CCCGCCATGT CCTTCTTCCG GCGGAAAGTG AAAGGCAAAG AACAAGAGAA GACCTCAGAT 
GTGAAGTCCA TTAAAGCTTC AATATCCGTA CATTCCCCAC AAAAAAGCAC TAAAAATCAT 
GCCTTGCTGG AGGCTGCAGG ACCAAGTCAT GTTGCAATCA ATGCCATTTC TGCCAACATG 
GACTCCTTTT CAAGTAGCAG GACAGCCACA CTTAAGAAGC AGCCAAGCCA CATGGAGGCC 
GCTCATTTTG GTGACCTGGG CAGATCTTGT CTGGACTACC AGACTCAAGA GACCAAATCA 
AGCCTTTCTA AGACCCTTGA ACAAGTCTTG CACGACACTA TTGTCCTCCC TTACTTCATT 
CAATTCATGG AACTTCGGCG AATGGAGCAT TTGGTGAAAT TTTGGTTAGA GGCTGAAAGT 
TTTCATTCAA CAACTTGGTC GCGAATAAGA GCACACAGTC TAAACACAGT GAAGCAGAGC 
TCACTGGCTG AGCCTGTCTC TCCATCTAAA AAGCATGAAA CTACAGCGTC TTTTTTAACT 
GATTCTCTTG ATAAGAGATT GGAGGATTCT GGCTCAGCAC AGTTGTTTAT GACTCATTCA 
GAAGGAATTG ACCTGAATAA TAGAACTAAC AGCACTCAGA ATCACTTGCT GCTTTCCCAG 
GAATGTGACA GTGCCCATTC TCTCCGTCTT GAAATGGCCA GAGCAGGAAC TCACCAAGTT 
TCCATGGAAA CCCAAGAATC TTCCTCTACA CTTACAGTAG CCAGTAGAAA TAGTCCCGCT 
TCTCCACTAA AAGAATTGTC AGGAAAACTA ATGAAAAGTA TAGAACAAGA TGCAGTGAAT 
ACTTTTACCA AATATATATC TCCAGATGCT GCTAAACCAA TACCAATTAC AGAAGCAATG 
AGAAATGACA TCATAGCAAG GATTTGTGGA GAAGATGGAC AGGTGGATCC CAACTGTTTC 
GTTTTGGCAC AGTCCATAGT CTTTAGTGCA ATGGAGCAAG AGCACTTTAG TGAGTTTCTG 
CGAAGTCACC ATTTCTGTAA ATACCAGATT GAAGTGCTGA CCAGTGGAAC TGTTTACCTG 
GCTGACATTC TCTTCTGTGA GTCAGCCCTC TTTTATTTCT CTGAGTACAT GGAAAAAGAG 
GATGCAGTGA ATATCTTACA ATTCTGGTTG GCAGCAGATA ACTTCCAGTC TCAGCTTGCT 
GCCAAAAAGG GCCAATATGA TGGACAGGAG GCACAGAATG ATGCCATGAT TTTATATGAC 
AAGTACTTCT CCCTCCAAGC CACACATCCT CTTGGATTTG ATGATGTTGT ACGATTAGAA 
ATTGAATCCA ATATCTGCAG GGAAGGTGGG CCACTCCCCA ACTGTTTCAC AACTCCATTA 
CGTCAGGCCT GGACAACCAT GGAGAAGGTC TTTTTGCCTG GCTTTTTGTC CAGCAATCTT 
TATTATAAAT ATTTGAATGA TCTCATCCAT TCGGTTCGAG GAGATGAATT TCTGGGCGGG 
AACGTGTCGC TGACTGCTCC TGGCTCTGTT GGCCCTCCTG ATGAGTCTCA CCCAGGGAGT 
TCTGACAGCT CTGCGTCTCA GTCCAGTGTG AAAAAAGCCA GTATTAAAAT ACTGAAAAAT 
TTTGATGAAG CGATAATTGT GGATGCGGCA AGTCTGGATC CAGAATCTTT ATATCAACGG 
ACATATGCCG GGAAGATGAC ATTTGGAAGA GTCAGTGACT TGGGGCAATT CATCCGAGAA 
TCTGAGCCTG AACCTGATGT AAGGAAATCA AAAGGATCCA TGTTCTCACA AGCTATGAAG 
AAATGGGTGC AAGGAAATAC TGATGAGGCC CAGGAAGAGC TAGCTTGGAA GATTGCTAAA 
ATGATAGTCA GTGACATTAT GCAGCAGGCT CAGTATGATC AACCGTTAGA GAAATCTACA 
AAGTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Erythropoietin signaling regulates heme biosynthesis.
eLife6(2017 05)
Chung J,Wittig JG,Ghamari A,Maeda M,Dailey TA,Bergonia H,Kafina MD,Coughlin EE,Minogue CE,Hebert AS,Li L,Kaplan J,Lodish HF,Bauer DE,Orkin SH,Cantor AB,Maeda T,Phillips JD,Coon JJ,Pagliarini DJ,Dailey HA,Paw BH

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M

Genetic association of AKAP10 gene polymorphism with reduced risk of preterm birth.
Journal of perinatology : official journal of the California Perinatal Association35(9)700-4(2015 Sep)
Langmia IM,Apalasamy YD,Suki SZ,Omar SZ,Mohamed Z

PKA RIα/A-kinase anchoring proteins 10 signaling pathway and the prognosis of colorectal cancer.
Journal of gastroenterology and hepatology30(3)496-503(2015 Mar)
Wang M,Li Y,Wang R,Wang Z,Chen K,Zhou B,Zhou Z,Sun X

D-AKAP2:PKA RII:PDZK1 ternary complex structure: insights from the nucleation of a polyvalent scaffold.
Protein science : a publication of the Protein Society24(1)105-16(2015 Jan)
Sarma GN,Moody IS,Ilouz R,Phan RH,Sankaran B,Hall RA,Taylor SS

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AKAP10 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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