MYL12B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYL12B
Entrez Gene ID	103910
Full Name	myosin light chain 12B
Synonyms	MLC-B,MRLC2
General protein information	Preferred Names myosin light chain 12B Names myosin regulatory light chain 12B MLC-2 MLC-2A MLC20 SHUJUN-1 myosin regulatory light chain 2 myosin regulatory light chain 2-B, smooth muscle isoform myosin regulatory light chain 20 kDa myosin regulatory light chain MRLC2 myosin, light chain 12B, regulatory
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 18 Map Location: 18p11.31
Summary	The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments (Iwasaki et al., 2001 [PubMed 11942626]).[supplied by OMIM, Mar 2008].
Disorder MIM:	609211

mRNA and Protein(s)

mRNA	Protein	Name
NM_001144944.1	NP_001138416.1	myosin regulatory light chain 12B
NM_033546.3	NP_291024.1	myosin regulatory light chain 12B
NM_001144945.1	NP_001138417.1	myosin regulatory light chain 12B

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MYL12A	NP_001015640.1
Caenorhabditis elegans (roundworm)	mlc-4	NP_497700.1
Mus musculus (house mouse)	Myl12a	NP_080340.2
Danio rerio (zebrafish)	myl12.2	NP_001124061.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000927	XP_003437136.1
Gallus gallus (chicken)	MYL12A	NP_990672.1
Drosophila melanogaster (fruit fly)	sqh	NP_511057.1
Xenopus tropicalis (tropical clawed frog)	myl12b	NP_001004813.1
Homo sapiens (human)	MYL12B	NP_291024.1
Pan troglodytes (chimpanzee)	MYL12B	XP_512040.1
Macaca mulatta (Rhesus monkey)	MYL12B	XP_001084275.1
Canis lupus familiaris (dog)	MYL12A	XP_848839.1
Rattus norvegicus (Norway rat)	Myl12a	NP_001128489.1
Gallus gallus (chicken)	LOC770011	XP_004939810.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide data reveal novel genes for methotrexate response in a large cohort of juvenile idiopathic arthritis cases.
Cobb J, Cule E, Moncrieffe H, Hinks A, Ursu S, Patrick F, Kassoumeri L, Flynn E, Bulatović M, Wulffraat N, van Zelst B, de Jonge R, Bohm M, Dolezalova P, Hirani S, Newman S, Whitworth P, Southwood TR, De Iorio M, , , , Wedderburn LR, Thomson W
The pharmacogenomics journal14(4)356-64(2014 Aug)

Cortical dynein and asymmetric membrane elongation coordinately position the spindle in anaphase.
Kiyomitsu T, Cheeseman IM
Cell154(2)391-402(2013 Jul)

Genetically distinct subsets within ANCA-associated vasculitis.
Lyons PA, Rayner TF, Trivedi S, Holle JU, Watts RA, Jayne DR, Baslund B, Brenchley P, Bruchfeld A, Chaudhry AN, Cohen Tervaert JW, Deloukas P, Feighery C, Gross WL, Guillevin L, Gunnarsson I, Harper L, Hrušková Z, Little MA, Martorana D, Neumann T, Ohlsson S, Padmanabhan S, Pusey CD, Salama AD, Sanders JS, Savage CO, Segelmark M, Stegeman CA, Tesař V, Vaglio A, Wieczorek S, Wilde B, Zwerina J, Rees AJ, Clayton DG, Smith KG
The New England journal of medicine367(3)214-23(2012 Jul)

Phosphorylation of myosin II regulatory light chain controls its accumulation, not that of actin, at the contractile ring in HeLa cells.
Kondo T, Itakura S, Hamao K, Hosoya H
Experimental cell research318(8)915-24(2012 May)

Diphosphorylated but not monophosphorylated myosin II regulatory light chain localizes to the midzone without its heavy chain during cytokinesis.
Kondo T, Isoda R, Uchimura T, Sugiyama M, Hamao K, Hosoya H
Biochemical and biophysical research communications417(2)686-91(2012 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MYL12B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYL12B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02314	NM_001144944.1 Latest version!	Homo sapiens myosin light chain 12B (MYL12B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu02314	NM_033546.3 Latest version!	Homo sapiens myosin light chain 12B (MYL12B), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00
OHu02314	NM_001144945.1 Latest version!	Homo sapiens myosin light chain 12B (MYL12B), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYL12B ( NM_001144944.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001138416.1 Homo sapiens myosin light chain 12B (MYL12B), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02314
Clone ID Related Accession (Same CDS sequence)	NM_001144944.1 , NM_033546.3 , NM_001144945.1
Accession Version	NM_001144944.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571328000000
Organism	Homo sapiens(human)
Product	myosin regulatory light chain 12B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG696903.1 and CA307094.1. Transcript Variant: This variant (1) represents the longest transcript. Variants 1-3 all encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BG696903.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAGCA AAAAGGCAAA GACCAAGACC ACCAAGAAGC GCCCTCAGCG TGCAACATCC 
AATGTGTTTG CCATGTTTGA CCAGTCACAG ATTCAGGAGT TCAAAGAGGC CTTCAACATG 
ATTGATCAGA ACAGAGATGG CTTCATCGAC AAGGAAGATT TGCATGATAT GCTTGCTTCT 
CTAGGGAAGA ATCCCACTGA TGCATACCTT GATGCCATGA TGAATGAGGC CCCAGGGCCC 
ATCAATTTCA CCATGTTCCT GACCATGTTT GGTGAGAAGT TAAATGGCAC AGATCCTGAA 
GATGTCATCA GAAACGCCTT TGCTTGCTTT GATGAAGAAG CAACAGGCAC CATTCAGGAA 
GATTACCTAA GAGAGCTGCT GACAACCATG GGGGATCGGT TTACAGATGA GGAAGTGGAT 
GAGCTGTACA GAGAAGCACC TATTGACAAA AAGGGGAATT TCAATTACAT CGAGTTCACA 
CGCATCCTGA AACATGGAGC CAAAGACAAA GATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138416.1
CDS	160..678
Translation	MSSKKAKTKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKDD

Target ORF information:

RefSeq Version	NM_001144944.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin light chain 12B (MYL12B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144944.1

ORF Insert Sequence:

ATGTCGAGCA AAAAGGCAAA GACCAAGACC ACCAAGAAGC GCCCTCAGCG TGCAACATCC 
AATGTGTTTG CCATGTTTGA CCAGTCACAG ATTCAGGAGT TCAAAGAGGC CTTCAACATG 
ATTGATCAGA ACAGAGATGG CTTCATCGAC AAGGAAGATT TGCATGATAT GCTTGCTTCT 
CTAGGGAAGA ATCCCACTGA TGCATACCTT GATGCCATGA TGAATGAGGC CCCAGGGCCC 
ATCAATTTCA CCATGTTCCT GACCATGTTT GGTGAGAAGT TAAATGGCAC AGATCCTGAA 
GATGTCATCA GAAACGCCTT TGCTTGCTTT GATGAAGAAG CAACAGGCAC CATTCAGGAA 
GATTACCTAA GAGAGCTGCT GACAACCATG GGGGATCGGT TTACAGATGA GGAAGTGGAT 
GAGCTGTACA GAGAAGCACC TATTGACAAA AAGGGGAATT TCAATTACAT CGAGTTCACA 
CGCATCCTGA AACATGGAGC CAAAGACAAA GATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYL12B ( NM_033546.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_291024.1 Homo sapiens myosin light chain 12B (MYL12B), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02314
Clone ID Related Accession (Same CDS sequence)	NM_001144944.1 , NM_033546.3 , NM_001144945.1
Accession Version	NM_033546.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Homo sapiens(human)
Product	myosin regulatory light chain 12B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG703257.1, AP005329.3 and CA307094.1. On Jan 30, 2009 this sequence version replaced NM_033546.2. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1-3 all encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.686125.1, SRR1660807.245483.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCGAGCA AAAAGGCAAA GACCAAGACC ACCAAGAAGC GCCCTCAGCG TGCAACATCC 
AATGTGTTTG CCATGTTTGA CCAGTCACAG ATTCAGGAGT TCAAAGAGGC CTTCAACATG 
ATTGATCAGA ACAGAGATGG CTTCATCGAC AAGGAAGATT TGCATGATAT GCTTGCTTCT 
CTAGGGAAGA ATCCCACTGA TGCATACCTT GATGCCATGA TGAATGAGGC CCCAGGGCCC 
ATCAATTTCA CCATGTTCCT GACCATGTTT GGTGAGAAGT TAAATGGCAC AGATCCTGAA 
GATGTCATCA GAAACGCCTT TGCTTGCTTT GATGAAGAAG CAACAGGCAC CATTCAGGAA 
GATTACCTAA GAGAGCTGCT GACAACCATG GGGGATCGGT TTACAGATGA GGAAGTGGAT 
GAGCTGTACA GAGAAGCACC TATTGACAAA AAGGGGAATT TCAATTACAT CGAGTTCACA 
CGCATCCTGA AACATGGAGC CAAAGACAAA GATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_291024.1
CDS	141..659
Translation	MSSKKAKTKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKDD

Target ORF information:

RefSeq Version	NM_033546.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin light chain 12B (MYL12B), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033546.3

ORF Insert Sequence:

ATGTCGAGCA AAAAGGCAAA GACCAAGACC ACCAAGAAGC GCCCTCAGCG TGCAACATCC 
AATGTGTTTG CCATGTTTGA CCAGTCACAG ATTCAGGAGT TCAAAGAGGC CTTCAACATG 
ATTGATCAGA ACAGAGATGG CTTCATCGAC AAGGAAGATT TGCATGATAT GCTTGCTTCT 
CTAGGGAAGA ATCCCACTGA TGCATACCTT GATGCCATGA TGAATGAGGC CCCAGGGCCC 
ATCAATTTCA CCATGTTCCT GACCATGTTT GGTGAGAAGT TAAATGGCAC AGATCCTGAA 
GATGTCATCA GAAACGCCTT TGCTTGCTTT GATGAAGAAG CAACAGGCAC CATTCAGGAA 
GATTACCTAA GAGAGCTGCT GACAACCATG GGGGATCGGT TTACAGATGA GGAAGTGGAT 
GAGCTGTACA GAGAAGCACC TATTGACAAA AAGGGGAATT TCAATTACAT CGAGTTCACA 
CGCATCCTGA AACATGGAGC CAAAGACAAA GATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYL12B ( NM_001144945.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001138417.1 Homo sapiens myosin light chain 12B (MYL12B), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu02314
Clone ID Related Accession (Same CDS sequence)	NM_001144944.1 , NM_033546.3 , NM_001144945.1
Accession Version	NM_001144945.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571328000000
Organism	Homo sapiens(human)
Product	myosin regulatory light chain 12B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AP005329.3, CN302451.1 and CA307094.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1-3 all encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The extent of this RefSeq transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BQ420428.1, SRR1163657.11460.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGAGCA AAAAGGCAAA GACCAAGACC ACCAAGAAGC GCCCTCAGCG TGCAACATCC 
AATGTGTTTG CCATGTTTGA CCAGTCACAG ATTCAGGAGT TCAAAGAGGC CTTCAACATG 
ATTGATCAGA ACAGAGATGG CTTCATCGAC AAGGAAGATT TGCATGATAT GCTTGCTTCT 
CTAGGGAAGA ATCCCACTGA TGCATACCTT GATGCCATGA TGAATGAGGC CCCAGGGCCC 
ATCAATTTCA CCATGTTCCT GACCATGTTT GGTGAGAAGT TAAATGGCAC AGATCCTGAA 
GATGTCATCA GAAACGCCTT TGCTTGCTTT GATGAAGAAG CAACAGGCAC CATTCAGGAA 
GATTACCTAA GAGAGCTGCT GACAACCATG GGGGATCGGT TTACAGATGA GGAAGTGGAT 
GAGCTGTACA GAGAAGCACC TATTGACAAA AAGGGGAATT TCAATTACAT CGAGTTCACA 
CGCATCCTGA AACATGGAGC CAAAGACAAA GATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138417.1
CDS	82..600
Translation	MSSKKAKTKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKDD

Target ORF information:

RefSeq Version	NM_001144945.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin light chain 12B (MYL12B), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144945.1

ORF Insert Sequence:

ATGTCGAGCA AAAAGGCAAA GACCAAGACC ACCAAGAAGC GCCCTCAGCG TGCAACATCC 
AATGTGTTTG CCATGTTTGA CCAGTCACAG ATTCAGGAGT TCAAAGAGGC CTTCAACATG 
ATTGATCAGA ACAGAGATGG CTTCATCGAC AAGGAAGATT TGCATGATAT GCTTGCTTCT 
CTAGGGAAGA ATCCCACTGA TGCATACCTT GATGCCATGA TGAATGAGGC CCCAGGGCCC 
ATCAATTTCA CCATGTTCCT GACCATGTTT GGTGAGAAGT TAAATGGCAC AGATCCTGAA 
GATGTCATCA GAAACGCCTT TGCTTGCTTT GATGAAGAAG CAACAGGCAC CATTCAGGAA 
GATTACCTAA GAGAGCTGCT GACAACCATG GGGGATCGGT TTACAGATGA GGAAGTGGAT 
GAGCTGTACA GAGAAGCACC TATTGACAAA AAGGGGAATT TCAATTACAT CGAGTTCACA 
CGCATCCTGA AACATGGAGC CAAAGACAAA GATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide data reveal novel genes for methotrexate response in a large cohort of juvenile idiopathic arthritis cases.
The pharmacogenomics journal14(4)356-64(2014 Aug)
Cobb J,Cule E,Moncrieffe H,Hinks A,Ursu S,Patrick F,Kassoumeri L,Flynn E,Bulatović M,Wulffraat N,van Zelst B,de Jonge R,Bohm M,Dolezalova P,Hirani S,Newman S,Whitworth P,Southwood TR,De Iorio M,,,,Wedderburn LR,Thomson W

Cortical dynein and asymmetric membrane elongation coordinately position the spindle in anaphase.
Cell154(2)391-402(2013 Jul)
Kiyomitsu T,Cheeseman IM

Genetically distinct subsets within ANCA-associated vasculitis.
The New England journal of medicine367(3)214-23(2012 Jul)
Lyons PA,Rayner TF,Trivedi S,Holle JU,Watts RA,Jayne DR,Baslund B,Brenchley P,Bruchfeld A,Chaudhry AN,Cohen Tervaert JW,Deloukas P,Feighery C,Gross WL,Guillevin L,Gunnarsson I,Harper L,Hrušková Z,Little MA,Martorana D,Neumann T,Ohlsson S,Padmanabhan S,Pusey CD,Salama AD,Sanders JS,Savage CO,Segelmark M,Stegeman CA,Tesař V,Vaglio A,Wieczorek S,Wilde B,Zwerina J,Rees AJ,Clayton DG,Smith KG

Phosphorylation of myosin II regulatory light chain controls its accumulation, not that of actin, at the contractile ring in HeLa cells.
Experimental cell research318(8)915-24(2012 May)
Kondo T,Itakura S,Hamao K,Hosoya H

Diphosphorylated but not monophosphorylated myosin II regulatory light chain localizes to the midzone without its heavy chain during cytokinesis.
Biochemical and biophysical research communications417(2)686-91(2012 Jan)
Kondo T,Isoda R,Uchimura T,Sugiyama M,Hamao K,Hosoya H

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MYL12B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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