CHAF1A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHAF1A
Entrez Gene ID	10036
Full Name	chromatin assembly factor 1 subunit A
Synonyms	CAF-1,CAF1,CAF1B,CAF1P150,P150
General protein information	Preferred Names chromatin assembly factor 1 subunit A Names chromatin assembly factor 1 subunit A CAF-1 subunit A CAF-I 150 kDa subunit CAF-I p150 CTB-50L17.7 chromatin assembly factor I (150 kDa) chromatin assembly factor I p150 subunit hp150
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.3
Summary	Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).[supplied by OMIM, Mar 2008].
Disorder MIM:	601246

mRNA and Protein(s)

mRNA	Protein	Name
NM_005483.3	NP_005474.2	chromatin assembly factor 1 subunit A
NM_005483.2	NP_005474.2	chromatin assembly factor 1 subunit A
XM_011527606.2	XP_011525908.1	chromatin assembly factor 1 subunit A isoform X2
XM_011527605.2	XP_011525907.1	chromatin assembly factor 1 subunit A isoform X1
XM_011527607.2	XP_011525909.1	chromatin assembly factor 1 subunit A isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CHAF1A	XP_512286.3
Macaca mulatta (Rhesus monkey)	CHAF1A	XP_002801077.1
Bos taurus (cattle)	CHAF1A	NP_001095312.1
Rattus norvegicus (Norway rat)	Chaf1a	XP_343672.2
Gallus gallus (chicken)	CHAF1A	NP_001073220.2
Canis lupus familiaris (dog)	CHAF1A	XP_542156.4
Mus musculus (house mouse)	Chaf1a	NP_038761.1
Xenopus tropicalis (tropical clawed frog)	LOC100485264	XP_002934050.1
Homo sapiens (human)	CHAF1A	NP_005474.2

Gene Ontology
Bibliography

Related articles in PubMed

CHAF1A interacts with TCF4 to promote gastric carcinogenesis via upregulation of c-MYC and CCND1 expression.
Zheng L, Liang X, Li S, Li T, Shang W, Ma L, Jia X, Shao W, Sun P, Chen C, Jia J
EBioMedicine3869-78(2018 Dec)

NuRD and CAF-1-mediated silencing of the D4Z4 array is modulated by DUX4-induced MBD3L proteins.
Campbell AE, Shadle SC, Jagannathan S, Lim JW, Resnick R, Tawil R, van der Maarel SM, Tapscott SJ
eLife7(2018 03)

[Highly expressed CHAF1A and PCNA are positively associated with malignancy of cervical squamous cell carcinoma].
Luo Y, Wang Q, Tian P, Jia Y
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology33(12)1696-1701(2017 Dec)

Emerging roles of the histone chaperone CAF-1 in cellular plasticity.
Cheloufi S, Hochedlinger K
Current opinion in genetics & development4683-94(2017 Oct)

Over-expression of CHAF1A in Epithelial Ovarian Cancer can promote cell proliferation and inhibit cell apoptosis.
Xia D, Yang X, Liu W, Shen F, Pan J, Lin Y, Du N, Sun Y, Xi X
Biochemical and biophysical research communications486(1)191-197(2017 04)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CHAF1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHAF1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu63618	XM_011527605.2 Latest version!	Homo sapiens chromatin assembly factor 1 subunit A (CHAF1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu63619	XM_011527606.2 Latest version!	Homo sapiens chromatin assembly factor 1 subunit A (CHAF1A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu63620	XM_011527607.2 Latest version!	Homo sapiens chromatin assembly factor 1 subunit A (CHAF1A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu16589	NM_005483.3 Latest version!	Homo sapiens chromatin assembly factor 1 subunit A (CHAF1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$475.30 $679.00
View Old Accession Versions >>
OHu16589	NM_005483.2	Homo sapiens chromatin assembly factor 1 subunit A (CHAF1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHAF1A ( XM_011527605.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011525907.1 Homo sapiens chromatin assembly factor 1 subunit A (CHAF1A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu63618
Clone ID Related Accession (Same CDS sequence)	XM_011527605.2
Accession Version	XM_011527605.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	chromatin assembly factor 1 subunit A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011527605.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAGG AGCTGGAGTG CGGGGCGCCC GGCGCCAGGG GAGCCGCCAC AGCCATGGAT 
TGCAAAGATA GACCAGCTTT TCCAGTTAAG AAGTTAATAC AAGCCCGTCT GCCGTTTAAG 
CGCCTGAATC TTGTCCCAAA GGGGAAAGCC GATGACATGT CAGACGATCA GGGTACTTCT 
GTGCAAAGTA AAAGCCCCGA TTTAGAGGCC TCTTTGGACA CCTTGGAAAA CAACTGTCAT 
GTGGGTTCTG ACATAGACTT TAGACCGAAA CTTGTCAACG GGAAGGGTCC CTTAGATAAC 
TTTTTAAGAA ATAGAATCGA AACCAGTATT GGCCAGAGCA CAGTCATCAT TGATTTGACA 
GAGGACTCGA ATGAGCAGCC AGACAGTCTT GTGGACCACA ATAAACTAAA TTCTGAAGCC 
TCTCCCTCCA GGGAGGCAAT AAATGGCCAG CGAGAAGACA CTGGGGATCA GCAGGGGTTG 
TTGAAGGCCA TTCAGAACGA CAAGTTGGCA TTTCCTGGAG AGACCCTTTC AGACATTCCT 
TGCAAAACAG AGGAGGAGGG TGTTGGCTGT GGAGGTGCAG GGAGGAGAGG CGACTCCCAG 
GAATGTTCGC CACGGAGCTG CCCGGAGCTG ACGAGTGGCC CGAGAATGTG CCCCAGAAAG 
GAGCAGGACA GTTGGAGTGA AGCTGGGGGC ATCCTGTTCA AAGGGAAGGT GCCTATGGTG 
GTCTTGCAGG ACATCTTGGC TGTGAGACCA CCGCAAATCA AGTCCCTTCC AGCCACACCC 
CAAGGCAAGA ACATGACCCC TGAGAGTGAG GTGCTGGAAT CTTTCCCCGA AGAAGACTCT 
GTACTCAGCC ATTCGTCCCT GAGCTCTCCC TCTTCCACCA GCTCGCCCGA GGGGCCGCCT 
GCTCCCCCAA AGCAGCACAG CAGTACCAGT CCCTTCCCCA CCTCCACGCC CCTCCGCAGA 
ATAACTAAGA AATTCGTCAA AGGCTCTACA GAGAAGAACA AGCTCAGACT GCAAAGAGAT 
CAGGAGCGTC TGGGCAAGCA GCTCAAGTTA CGTGCAGAAA GGGAAGAAAA GGAGAAGCTG 
AAAGAGGAGG CCAAGCGGGC CAAGGAGGAG GCCAAGAAGA AGAAGGAGGA AGAGAAGGAG 
CTTAAGGAAA AGGAGAGGCG GGAGAAGCGG GAGAAGGATG AGAAGGAGAA GGCGGAGAAG 
CAGCGGCTCA AGGAGGAGCG GCGCAAGGAG AGACAGGAAG CCCTGGAGGC TAAACTTGAG 
GAAAAAAGGA AAAAGGAAGA AGAGAAACGG TTAAGAGAAG AAGAGAAGCG CATTAAAGCA 
GAGAAGGCCG AAATCACGAG GTTCTTCCAG AAACCAAAGA CTCCACAGGC CCCCAAGACC 
CTGGCCGGCT CCTGTGGGAA GTTTGCCCCC TTTGAAATTA AAGAGCACAT GGTCCTGGCC 
CCTCGGCGTC GGACCGCTTT CCATCCAGAC CTCTGCAGTC AGCTGGACCA GCTCCTCCAG 
CAGCAGAGCG GCGAGTTCTC CTTCTTGAAA GACCTCAAAG GCCGGCAGCC CCTGAGGTCC 
GGACCCACGC ACGTTTCCAC CCGGAATGCA GATATTTTTA ACAGTGATGT CGTCATCGTG 
GAGCGTGGGA AGGGCGACGG TGTTCCCGAG AGGAGGAAGT TTGGCAGGAT GAAGCTCCTG 
CAGTTCTGTG AGAACCACCG GCCTGCCTAC TGGGGTACCT GGAATAAGAA GACGGCACTC 
ATCCGCGCGC GAGACCCCTG GGCCCAGGAC ACGAAGCTCC TGGACTATGA GGTGGACAGT 
GATGAGGAGT GGGAAGAAGA GGAGCCTGGG GAGTCCCTGT CCCACAGTGA GGGGGATGAT 
GATGACGACA TGGGAGAGGA TGAAGATGAG GACGATGGTT TCTTTGTGCC CCATGGGTAC 
CTGTCTGAGG ACGAAGGTGT GACAGAGGAG TGTGCCGACC CTGAGAACCA TAAGGTCCGC 
CAGAAACTGA AGGCCAAGGA GTGGGACGAG TTCCTGGCTA AGGGGAAGCG CTTTCGCGTC 
CTGCAACCTG TGAAGATCGG CTGCGTGTGG GCGGCTGACA GAGACTGCGC AGGCGATGAC 
CTGAAGGTAC TGCAGCAGTT CGCAGCCTGC TTCCTGGAGA CCCTGCCGGC CCAGGAGGAG 
CAGACGCCCA AGGCCTCCAA GCGGGAGAGG AGAGACGAGC AGATCCTGGC CCAGCTGCTG 
CCGCTCCTGC ACGGCAATGT GAACGGGAGC AAGGTCATCA TCCGGGAGTT CCAGGAGCAC 
TGCCGCCGGG GACTGCTCAG CAACCACACC GGCAGCCCGC GGAGCCCCTC CACCACCTAC 
CTGCACACCC CCACCCCCAG CGAGGATGCC GCCATCCCCT CTAAGTCCCG GCTCAAGCGG 
CTCATTTCCG AGAACTCAGT GTATGAGAAG CGGCCTGACT TCAGGATGTG CTGGTACGTG 
CACCCGCAGG TGCTACAGAG CTTCCAGCAG GAGCACCTGC CCGTGCCGTG CCAGTGGAGC 
TATGTGACAT CGGTGCCCTC GGCCCCCAAA GAGGACAGTG GCAGCGTCCC CTCCACGGGG 
CCCAGCCAGG GCACTCCCAT CTCGCTGAAG AGGAAGTCAG CGGGCAGCAT GTGCATCACC 
CAATTCATGA AGAAGCGCAG GCACGACGGC CAGATTGGTG CTGAAGACAT GGACGGCTTC 
CAGGCAGACA CGGAGGAGGA GGAAGAGGAG GAGGGCGACT GTATGATCGT GGATGTCCCG 
GATGCTGCGG TCTCCAACAG CTCTGCACAG AGGAAAGAGT CACAACCAGG CACGAAGAGT 
GAGGCTGAGG AGCCAGACAC TGCTGGGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011525907.1
CDS	168..3020
Translation	MLEELECGAPGARGAATAMDCKDRPAFPVKKLIQARLPFKRLNLVPKGKADDMSDDQGTSVQSKSPDLEASLDTLENNCHVGSDIDFRPKLVNGKGPLDNFLRNRIETSIGQSTVIIDLTEDSNEQPDSLVDHNKLNSEASPSREAINGQREDTGDQQGLLKAIQNDKLAFPGETLSDIPCKTEEEGVGCGGAGRRGDSQECSPRSCPELTSGPRMCPRKEQDSWSEAGGILFKGKVPMVVLQDILAVRPPQIKSLPATPQGKNMTPESEVLESFPEEDSVLSHSSLSSPSSTSSPEGPPAPPKQHSSTSPFPTSTPLRRITKKFVKGSTEKNKLRLQRDQERLGKQLKLRAEREEKEKLKEEAKRAKEEAKKKKEEEKELKEKERREKREKDEKEKAEKQRLKEERRKERQEALEAKLEEKRKKEEEKRLREEEKRIKAEKAEITRFFQKPKTPQAPKTLAGSCGKFAPFEIKEHMVLAPRRRTAFHPDLCSQLDQLLQQQSGEFSFLKDLKGRQPLRSGPTHVSTRNADIFNSDVVIVERGKGDGVPERRKFGRMKLLQFCENHRPAYWGTWNKKTALIRARDPWAQDTKLLDYEVDSDEEWEEEEPGESLSHSEGDDDDDMGEDEDEDDGFFVPHGYLSEDEGVTEECADPENHKVRQKLKAKEWDEFLAKGKRFRVLQPVKIGCVWAADRDCAGDDLKVLQQFAACFLETLPAQEEQTPKASKRERRDEQILAQLLPLLHGNVNGSKVIIREFQEHCRRGLLSNHTGSPRSPSTTYLHTPTPSEDAAIPSKSRLKRLISENSVYEKRPDFRMCWYVHPQVLQSFQQEHLPVPCQWSYVTSVPSAPKEDSGSVPSTGPSQGTPISLKRKSAGSMCITQFMKKRRHDGQIGAEDMDGFQADTEEEEEEEGDCMIVDVPDAAVSNSSAQRKESQPGTKSEAEEPDTAGA

Target ORF information:

RefSeq Version	XM_011527605.2
Organism	Homo sapiens(human)
Definition	Homo sapiens chromatin assembly factor 1 subunit A (CHAF1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527605.2

ORF Insert Sequence:

ATGCTGGAGG AGCTGGAGTG CGGGGCGCCC GGCGCCAGGG GAGCCGCCAC AGCCATGGAT 
TGCAAAGATA GACCAGCTTT TCCAGTTAAG AAGTTAATAC AAGCCCGTCT GCCGTTTAAG 
CGCCTGAATC TTGTCCCAAA GGGGAAAGCC GATGACATGT CAGACGATCA GGGTACTTCT 
GTGCAAAGTA AAAGCCCCGA TTTAGAGGCC TCTTTGGACA CCTTGGAAAA CAACTGTCAT 
GTGGGTTCTG ACATAGACTT TAGACCGAAA CTTGTCAACG GGAAGGGTCC CTTAGATAAC 
TTTTTAAGAA ATAGAATCGA AACCAGTATT GGCCAGAGCA CAGTCATCAT TGATTTGACA 
GAGGACTCGA ATGAGCAGCC AGACAGTCTT GTGGACCACA ATAAACTAAA TTCTGAAGCC 
TCTCCCTCCA GGGAGGCAAT AAATGGCCAG CGAGAAGACA CTGGGGATCA GCAGGGGTTG 
TTGAAGGCCA TTCAGAACGA CAAGTTGGCA TTTCCTGGAG AGACCCTTTC AGACATTCCT 
TGCAAAACAG AGGAGGAGGG TGTTGGCTGT GGAGGTGCAG GGAGGAGAGG CGACTCCCAG 
GAATGTTCGC CACGGAGCTG CCCGGAGCTG ACGAGTGGCC CGAGAATGTG CCCCAGAAAG 
GAGCAGGACA GTTGGAGTGA AGCTGGGGGC ATCCTGTTCA AAGGGAAGGT GCCTATGGTG 
GTCTTGCAGG ACATCTTGGC TGTGAGACCA CCGCAAATCA AGTCCCTTCC AGCCACACCC 
CAAGGCAAGA ACATGACCCC TGAGAGTGAG GTGCTGGAAT CTTTCCCCGA AGAAGACTCT 
GTACTCAGCC ATTCGTCCCT GAGCTCTCCC TCTTCCACCA GCTCGCCCGA GGGGCCGCCT 
GCTCCCCCAA AGCAGCACAG CAGTACCAGT CCCTTCCCCA CCTCCACGCC CCTCCGCAGA 
ATAACTAAGA AATTCGTCAA AGGCTCTACA GAGAAGAACA AGCTCAGACT GCAAAGAGAT 
CAGGAGCGTC TGGGCAAGCA GCTCAAGTTA CGTGCAGAAA GGGAAGAAAA GGAGAAGCTG 
AAAGAGGAGG CCAAGCGGGC CAAGGAGGAG GCCAAGAAGA AGAAGGAGGA AGAGAAGGAG 
CTTAAGGAAA AGGAGAGGCG GGAGAAGCGG GAGAAGGATG AGAAGGAGAA GGCGGAGAAG 
CAGCGGCTCA AGGAGGAGCG GCGCAAGGAG AGACAGGAAG CCCTGGAGGC TAAACTTGAG 
GAAAAAAGGA AAAAGGAAGA AGAGAAACGG TTAAGAGAAG AAGAGAAGCG CATTAAAGCA 
GAGAAGGCCG AAATCACGAG GTTCTTCCAG AAACCAAAGA CTCCACAGGC CCCCAAGACC 
CTGGCCGGCT CCTGTGGGAA GTTTGCCCCC TTTGAAATTA AAGAGCACAT GGTCCTGGCC 
CCTCGGCGTC GGACCGCTTT CCATCCAGAC CTCTGCAGTC AGCTGGACCA GCTCCTCCAG 
CAGCAGAGCG GCGAGTTCTC CTTCTTGAAA GACCTCAAAG GCCGGCAGCC CCTGAGGTCC 
GGACCCACGC ACGTTTCCAC CCGGAATGCA GATATTTTTA ACAGTGATGT CGTCATCGTG 
GAGCGTGGGA AGGGCGACGG TGTTCCCGAG AGGAGGAAGT TTGGCAGGAT GAAGCTCCTG 
CAGTTCTGTG AGAACCACCG GCCTGCCTAC TGGGGTACCT GGAATAAGAA GACGGCACTC 
ATCCGCGCGC GAGACCCCTG GGCCCAGGAC ACGAAGCTCC TGGACTATGA GGTGGACAGT 
GATGAGGAGT GGGAAGAAGA GGAGCCTGGG GAGTCCCTGT CCCACAGTGA GGGGGATGAT 
GATGACGACA TGGGAGAGGA TGAAGATGAG GACGATGGTT TCTTTGTGCC CCATGGGTAC 
CTGTCTGAGG ACGAAGGTGT GACAGAGGAG TGTGCCGACC CTGAGAACCA TAAGGTCCGC 
CAGAAACTGA AGGCCAAGGA GTGGGACGAG TTCCTGGCTA AGGGGAAGCG CTTTCGCGTC 
CTGCAACCTG TGAAGATCGG CTGCGTGTGG GCGGCTGACA GAGACTGCGC AGGCGATGAC 
CTGAAGGTAC TGCAGCAGTT CGCAGCCTGC TTCCTGGAGA CCCTGCCGGC CCAGGAGGAG 
CAGACGCCCA AGGCCTCCAA GCGGGAGAGG AGAGACGAGC AGATCCTGGC CCAGCTGCTG 
CCGCTCCTGC ACGGCAATGT GAACGGGAGC AAGGTCATCA TCCGGGAGTT CCAGGAGCAC 
TGCCGCCGGG GACTGCTCAG CAACCACACC GGCAGCCCGC GGAGCCCCTC CACCACCTAC 
CTGCACACCC CCACCCCCAG CGAGGATGCC GCCATCCCCT CTAAGTCCCG GCTCAAGCGG 
CTCATTTCCG AGAACTCAGT GTATGAGAAG CGGCCTGACT TCAGGATGTG CTGGTACGTG 
CACCCGCAGG TGCTACAGAG CTTCCAGCAG GAGCACCTGC CCGTGCCGTG CCAGTGGAGC 
TATGTGACAT CGGTGCCCTC GGCCCCCAAA GAGGACAGTG GCAGCGTCCC CTCCACGGGG 
CCCAGCCAGG GCACTCCCAT CTCGCTGAAG AGGAAGTCAG CGGGCAGCAT GTGCATCACC 
CAATTCATGA AGAAGCGCAG GCACGACGGC CAGATTGGTG CTGAAGACAT GGACGGCTTC 
CAGGCAGACA CGGAGGAGGA GGAAGAGGAG GAGGGCGACT GTATGATCGT GGATGTCCCG 
GATGCTGCGG TCTCCAACAG CTCTGCACAG AGGAAAGAGT CACAACCAGG CACGAAGAGT 
GAGGCTGAGG AGCCAGACAC TGCTGGGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHAF1A ( XM_011527606.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011525908.1 Homo sapiens chromatin assembly factor 1 subunit A (CHAF1A), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu63619
Clone ID Related Accession (Same CDS sequence)	XM_011527606.2
Accession Version	XM_011527606.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	chromatin assembly factor 1 subunit A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011527606.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAGG AGCTGGAGTG CGGGGCGCCC GGCGCCAGGG GAGCCGCCAC AGCCATGGAT 
TGCAAAGATA GACCAGCTTT TCCAGTTAAG AAGTTAATAC AAGCCCGTCT GCCGTTTAAG 
CGCCTGAATC TTGTCCCAAA GGGGAAAGCC GATGACATGT CAGACGATCA GGGTACTTCT 
GTGCAAAGTA AAAGCCCCGA TTTAGAGGCC TCTTTGGACA CCTTGGAAAA CAACTGTCAT 
GTGGGTTCTG ACATAGACTT TAGACCGAAA CTTGTCAACG GGAAGGGTCC CTTAGATAAC 
TTTTTAAGAA ATAGAATCGA AACCAGTATT GGCCAGAGCA CAGTCATCAT TGATTTGACA 
GAGGACTCGA ATGAGCAGCC AGACAGTCTT GTGGACCACA ATAAACTAAA TTCTGAAGCC 
TCTCCCTCCA GGGAGGCAAT AAATGGCCAG CGAGAAGACA CTGGGGATCA GCAGGGGTTG 
TTGAAGGCCA TTCAGAACGA CAAGTTGGCA TTTCCTGGAG AGACCCTTTC AGACATTCCT 
TGCAAAACAG AGGAGGAGGG TGTTGGCTGT GGAGGTGCAG GGAGGAGAGG CGACTCCCAG 
GAATGTTCGC CACGGAGCTG CCCGGAGCTG ACGAGTGGCC CGAGAATGTG CCCCAGAAAG 
GAGCAGGACA GTTGGAGTGA AGCTGGGGGC ATCCTGTTCA AAGGGAAGGT GCCTATGGTG 
GTCTTGCAGG ACATCTTGGC TGTGAGACCA CCGCAAATCA AGTCCCTTCC AGCCACACCC 
CAAGGCAAGA ACATGACCCC TGAGAGTGAG GTGCTGGAAT CTTTCCCCGA AGAAGACTCT 
GTACTCAGCC ATTCGTCCCT GAGCTCTCCC TCTTCCACCA GCTCGCCCGA GGGGCCGCCT 
GCTCCCCCAA AGCAGCACAG CAGTACCAGT CCCTTCCCCA CCTCCACGCC CCTCCGCAGA 
ATAACTAAGA AATTCGTCAA AGGCTCTACA GAGAAGAACA AGCTCAGACT GCAAAGAGAT 
CAGGAGCGTC TGGGCAAGCA GCTCAAGTTA CGTGCAGAAA GGGAAGAAAA GGAGAAGCTG 
AAAGAGGAGG CCAAGCGGGC CAAGGAGGAG GCCAAGAAGA AGAAGGAGGA AGAGAAGGAG 
CTTAAGGAAA AGGAGAGGCG GGAGAAGCGG GAGAAGGATG AGAAGGAGAA GGCGGAGAAG 
CAGCGGCTCA AGGAGGAGCG GCGCAAGGAG AGACAGGAAG CCCTGGAGGC TAAACTTGAG 
GAAAAAAGGA AAAAGGAAGA AGAGAAACGG TTAAGAGAAG AAGAGAAGCG CATTAAAGCA 
GAGAAGGCCG AAATCACGAG GTTCTTCCAG AAACCAAAGA CTCCACAGGC CCCCAAGACC 
CTGGCCGGCT CCTGTGGGAA GTTTGCCCCC TTTGAAATTA AAGAGCACAT GGTCCTGGCC 
CCTCGGCGTC GGACCGCTTT CCATCCAGAC CTCTGCAGTC AGCTGGACCA GCTCCTCCAG 
CAGCAGAGCG GCGAGTTCTC CTTCTTGAAA GACCTCAAAG GCCGGCAGCC CCTGAGGTCC 
GGACCCACGC ACGTTTCCAC CCGGAATGCA GATATTTTTA ACAGTGATGT CGTCATCGTG 
GAGCGTGGGA AGGGCGACGG TGTTCCCGAG AGGAGGAAGT TTGGCAGGAT GAAGCTCCTG 
CAGTTCTGTG AGAACCACCG GCCTGCCTAC TGGGGTACCT GGAATAAGAA GACGGCACTC 
ATCCGCGCGC GAGACCCCTG GGCCCAGGAC ACGAAGCTCC TGGACTATGA GGTGGACAGT 
GATGAGGAGT GGGAAGAAGA GGAGCCTGGG GAGTCCCTGT CCCACAGTGA GGGGGATGAT 
GATGACGACA TGGGAGAGGA TGAAGATGAG GACGATGGTT TCTTTGTGCC CCATGGGTAC 
CTGTCTGAGG ACGAAGGTGT GACAGAGGAG TGTGCCGACC CTGAGAACCA TAAGGTCCGC 
CAGAAACTGA AGGCCAAGGA GTGGGACGAG TTCCTGGCTA AGGGGAAGCG CTTTCGCGTC 
CTGCAACCTG TGAAGATCGG CTGCGTGTGG GCGGCTGACA GAGACTGCGC AGGCGATGAC 
CTGAAGGTAC TGCAGCAGTT CGCAGCCTGC TTCCTGGAGA CCCTGCCGGC CCAGGAGGAG 
CAGACGCCCA AGGCCTCCAA GCGGGAGAGG AGAGACGAGC AGATCCTGGC CCAGCTGCTG 
CCGCTCCTGC ACGGCAATGT GAACGGGAGC AAGGTCATCA TCCGGGAGTT CCAGGAGCAC 
TGCCGCCGGG GACTGCTCAG CAACCACACC GGCAGCCCGC GGAGCCCCTC CACCACCTAC 
CTGCACACCC CCACCCCCAG CGAGGATGCC GCCATCCCCT CTAAGTCCCG GCTCAAGCGG 
CTCATTTCCG AGAACTCAGT GTATGAGAAG CGGCCTGACT TCAGGATGTG CTGGTACGTG 
CACCCGCAGG TGCTACAGAG CTTCCAGCAG GAGCACCTGC CCGTGCCGTG CCAGTGGAGC 
TATGTGACAT CGGTGCCCTC GGCCCCCAAA GAGGACAGTG GCAGCGTCCC CTCCACGGGG 
CCCAGCCAGG GCACTCCCAT CTCGCTGAAG AGGAAGTCAG CGGGCAGCAT GTGCATCACC 
CAATTCATGA AGAAGCGCAG GCACGACGGC CAGATTGGTG CTGAAGACAT GGACGGCTTC 
CAGGCAGACA CGGAGGAGGA GGAAGAGGAG GAGGGCGACT GTATGATCGT GGATGTCCCG 
GATGCTGCGG CCATGCGCAG CCTCTTCCAG TCTCTCTCTG ATTCACCCTC CTGCCACCTC 
TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011525908.1
CDS	168..2993
Translation	MLEELECGAPGARGAATAMDCKDRPAFPVKKLIQARLPFKRLNLVPKGKADDMSDDQGTSVQSKSPDLEASLDTLENNCHVGSDIDFRPKLVNGKGPLDNFLRNRIETSIGQSTVIIDLTEDSNEQPDSLVDHNKLNSEASPSREAINGQREDTGDQQGLLKAIQNDKLAFPGETLSDIPCKTEEEGVGCGGAGRRGDSQECSPRSCPELTSGPRMCPRKEQDSWSEAGGILFKGKVPMVVLQDILAVRPPQIKSLPATPQGKNMTPESEVLESFPEEDSVLSHSSLSSPSSTSSPEGPPAPPKQHSSTSPFPTSTPLRRITKKFVKGSTEKNKLRLQRDQERLGKQLKLRAEREEKEKLKEEAKRAKEEAKKKKEEEKELKEKERREKREKDEKEKAEKQRLKEERRKERQEALEAKLEEKRKKEEEKRLREEEKRIKAEKAEITRFFQKPKTPQAPKTLAGSCGKFAPFEIKEHMVLAPRRRTAFHPDLCSQLDQLLQQQSGEFSFLKDLKGRQPLRSGPTHVSTRNADIFNSDVVIVERGKGDGVPERRKFGRMKLLQFCENHRPAYWGTWNKKTALIRARDPWAQDTKLLDYEVDSDEEWEEEEPGESLSHSEGDDDDDMGEDEDEDDGFFVPHGYLSEDEGVTEECADPENHKVRQKLKAKEWDEFLAKGKRFRVLQPVKIGCVWAADRDCAGDDLKVLQQFAACFLETLPAQEEQTPKASKRERRDEQILAQLLPLLHGNVNGSKVIIREFQEHCRRGLLSNHTGSPRSPSTTYLHTPTPSEDAAIPSKSRLKRLISENSVYEKRPDFRMCWYVHPQVLQSFQQEHLPVPCQWSYVTSVPSAPKEDSGSVPSTGPSQGTPISLKRKSAGSMCITQFMKKRRHDGQIGAEDMDGFQADTEEEEEEEGDCMIVDVPDAAAMRSLFQSLSDSPSCHLL

Target ORF information:

RefSeq Version	XM_011527606.2
Organism	Homo sapiens(human)
Definition	Homo sapiens chromatin assembly factor 1 subunit A (CHAF1A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527606.2

ORF Insert Sequence:

ATGCTGGAGG AGCTGGAGTG CGGGGCGCCC GGCGCCAGGG GAGCCGCCAC AGCCATGGAT 
TGCAAAGATA GACCAGCTTT TCCAGTTAAG AAGTTAATAC AAGCCCGTCT GCCGTTTAAG 
CGCCTGAATC TTGTCCCAAA GGGGAAAGCC GATGACATGT CAGACGATCA GGGTACTTCT 
GTGCAAAGTA AAAGCCCCGA TTTAGAGGCC TCTTTGGACA CCTTGGAAAA CAACTGTCAT 
GTGGGTTCTG ACATAGACTT TAGACCGAAA CTTGTCAACG GGAAGGGTCC CTTAGATAAC 
TTTTTAAGAA ATAGAATCGA AACCAGTATT GGCCAGAGCA CAGTCATCAT TGATTTGACA 
GAGGACTCGA ATGAGCAGCC AGACAGTCTT GTGGACCACA ATAAACTAAA TTCTGAAGCC 
TCTCCCTCCA GGGAGGCAAT AAATGGCCAG CGAGAAGACA CTGGGGATCA GCAGGGGTTG 
TTGAAGGCCA TTCAGAACGA CAAGTTGGCA TTTCCTGGAG AGACCCTTTC AGACATTCCT 
TGCAAAACAG AGGAGGAGGG TGTTGGCTGT GGAGGTGCAG GGAGGAGAGG CGACTCCCAG 
GAATGTTCGC CACGGAGCTG CCCGGAGCTG ACGAGTGGCC CGAGAATGTG CCCCAGAAAG 
GAGCAGGACA GTTGGAGTGA AGCTGGGGGC ATCCTGTTCA AAGGGAAGGT GCCTATGGTG 
GTCTTGCAGG ACATCTTGGC TGTGAGACCA CCGCAAATCA AGTCCCTTCC AGCCACACCC 
CAAGGCAAGA ACATGACCCC TGAGAGTGAG GTGCTGGAAT CTTTCCCCGA AGAAGACTCT 
GTACTCAGCC ATTCGTCCCT GAGCTCTCCC TCTTCCACCA GCTCGCCCGA GGGGCCGCCT 
GCTCCCCCAA AGCAGCACAG CAGTACCAGT CCCTTCCCCA CCTCCACGCC CCTCCGCAGA 
ATAACTAAGA AATTCGTCAA AGGCTCTACA GAGAAGAACA AGCTCAGACT GCAAAGAGAT 
CAGGAGCGTC TGGGCAAGCA GCTCAAGTTA CGTGCAGAAA GGGAAGAAAA GGAGAAGCTG 
AAAGAGGAGG CCAAGCGGGC CAAGGAGGAG GCCAAGAAGA AGAAGGAGGA AGAGAAGGAG 
CTTAAGGAAA AGGAGAGGCG GGAGAAGCGG GAGAAGGATG AGAAGGAGAA GGCGGAGAAG 
CAGCGGCTCA AGGAGGAGCG GCGCAAGGAG AGACAGGAAG CCCTGGAGGC TAAACTTGAG 
GAAAAAAGGA AAAAGGAAGA AGAGAAACGG TTAAGAGAAG AAGAGAAGCG CATTAAAGCA 
GAGAAGGCCG AAATCACGAG GTTCTTCCAG AAACCAAAGA CTCCACAGGC CCCCAAGACC 
CTGGCCGGCT CCTGTGGGAA GTTTGCCCCC TTTGAAATTA AAGAGCACAT GGTCCTGGCC 
CCTCGGCGTC GGACCGCTTT CCATCCAGAC CTCTGCAGTC AGCTGGACCA GCTCCTCCAG 
CAGCAGAGCG GCGAGTTCTC CTTCTTGAAA GACCTCAAAG GCCGGCAGCC CCTGAGGTCC 
GGACCCACGC ACGTTTCCAC CCGGAATGCA GATATTTTTA ACAGTGATGT CGTCATCGTG 
GAGCGTGGGA AGGGCGACGG TGTTCCCGAG AGGAGGAAGT TTGGCAGGAT GAAGCTCCTG 
CAGTTCTGTG AGAACCACCG GCCTGCCTAC TGGGGTACCT GGAATAAGAA GACGGCACTC 
ATCCGCGCGC GAGACCCCTG GGCCCAGGAC ACGAAGCTCC TGGACTATGA GGTGGACAGT 
GATGAGGAGT GGGAAGAAGA GGAGCCTGGG GAGTCCCTGT CCCACAGTGA GGGGGATGAT 
GATGACGACA TGGGAGAGGA TGAAGATGAG GACGATGGTT TCTTTGTGCC CCATGGGTAC 
CTGTCTGAGG ACGAAGGTGT GACAGAGGAG TGTGCCGACC CTGAGAACCA TAAGGTCCGC 
CAGAAACTGA AGGCCAAGGA GTGGGACGAG TTCCTGGCTA AGGGGAAGCG CTTTCGCGTC 
CTGCAACCTG TGAAGATCGG CTGCGTGTGG GCGGCTGACA GAGACTGCGC AGGCGATGAC 
CTGAAGGTAC TGCAGCAGTT CGCAGCCTGC TTCCTGGAGA CCCTGCCGGC CCAGGAGGAG 
CAGACGCCCA AGGCCTCCAA GCGGGAGAGG AGAGACGAGC AGATCCTGGC CCAGCTGCTG 
CCGCTCCTGC ACGGCAATGT GAACGGGAGC AAGGTCATCA TCCGGGAGTT CCAGGAGCAC 
TGCCGCCGGG GACTGCTCAG CAACCACACC GGCAGCCCGC GGAGCCCCTC CACCACCTAC 
CTGCACACCC CCACCCCCAG CGAGGATGCC GCCATCCCCT CTAAGTCCCG GCTCAAGCGG 
CTCATTTCCG AGAACTCAGT GTATGAGAAG CGGCCTGACT TCAGGATGTG CTGGTACGTG 
CACCCGCAGG TGCTACAGAG CTTCCAGCAG GAGCACCTGC CCGTGCCGTG CCAGTGGAGC 
TATGTGACAT CGGTGCCCTC GGCCCCCAAA GAGGACAGTG GCAGCGTCCC CTCCACGGGG 
CCCAGCCAGG GCACTCCCAT CTCGCTGAAG AGGAAGTCAG CGGGCAGCAT GTGCATCACC 
CAATTCATGA AGAAGCGCAG GCACGACGGC CAGATTGGTG CTGAAGACAT GGACGGCTTC 
CAGGCAGACA CGGAGGAGGA GGAAGAGGAG GAGGGCGACT GTATGATCGT GGATGTCCCG 
GATGCTGCGG CCATGCGCAG CCTCTTCCAG TCTCTCTCTG ATTCACCCTC CTGCCACCTC 
TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHAF1A ( XM_011527607.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011525909.1 Homo sapiens chromatin assembly factor 1 subunit A (CHAF1A), transcript variant X4, mRNA.

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CloneID	OHu63620
Clone ID Related Accession (Same CDS sequence)	XM_011527607.2
Accession Version	XM_011527607.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	chromatin assembly factor 1 subunit A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011527607.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAGG AGCTGGAGTG CGGGGCGCCC GGCGCCAGGG GAGCCGCCAC AGCCATGGAT 
TGCAAAGATA GACCAGCTTT TCCAGTTAAG AAGTTAATAC AAGCCCGTCT GCCGTTTAAG 
CGCCTGAATC TTGTCCCAAA GGGGAAAGCC GATGACATGT CAGACGATCA GGGTACTTCT 
GTGCAAAGTA AAAGCCCCGA TTTAGAGGCC TCTTTGGACA CCTTGGAAAA CAACTGTCAT 
GTGGGTTCTG ACATAGACTT TAGACCGAAA CTTGTCAACG GGAAGGGTCC CTTAGATAAC 
TTTTTAAGAA ATAGAATCGA AACCAGTATT GGCCAGAGCA CAGTCATCAT TGATTTGACA 
GAGGACTCGA ATGAGCAGCC AGACAGTCTT GTGGACCACA ATAAACTAAA TTCTGAAGCC 
TCTCCCTCCA GGGAGGCAAT AAATGGCCAG CGAGAAGACA CTGGGGATCA GCAGGGGTTG 
TTGAAGGCCA TTCAGAACGA CAAGTTGGCA TTTCCTGGAG AGACCCTTTC AGACATTCCT 
TGCAAAACAG AGGAGGAGGG TGTTGGCTGT GGAGGTGCAG GGAGGAGAGG CGACTCCCAG 
GAATGTTCGC CACGGAGCTG CCCGGAGCTG ACGAGTGGCC CGAGAATGTG CCCCAGAAAG 
GAGCAGGACA GTTGGAGTGA AGCTGGGGGC ATCCTGTTCA AAGGGAAGGT GCCTATGGTG 
GTCTTGCAGG ACATCTTGGC TGTGAGACCA CCGCAAATCA AGTCCCTTCC AGCCACACCC 
CAAGGCAAGA ACATGACCCC TGAGAGTGAG GTGCTGGAAT CTTTCCCCGA AGAAGACTCT 
GTACTCAGCC ATTCGTCCCT GAGCTCTCCC TCTTCCACCA GCTCGCCCGA GGGGCCGCCT 
GCTCCCCCAA AGCAGCACAG CAGTACCAGT CCCTTCCCCA CCTCCACGCC CCTCCGCAGA 
GATCAGGAGC GTCTGGGCAA GCAGCTCAAG TTACGTGCAG AAAGGGAAGA AAAGGAGAAG 
CTGAAAGAGG AGGCCAAGCG GGCCAAGGAG GAGGCCAAGA AGAAGAAGGA GGAAGAGAAG 
GAGCTTAAGG AAAAGGAGAG GCGGGAGAAG CGGGAGAAGG ATGAGAAGGA GAAGGCGGAG 
AAGCAGCGGC TCAAGGAGGA GCGGCGCAAG GAGAGACAGG AAGCCCTGGA GGCTAAACTT 
GAGGAAAAAA GGAAAAAGGA AGAAGAGAAA CGGTTAAGAG AAGAAGAGAA GCGCATTAAA 
GCAGAGAAGG CCGAAATCAC GAGGTTCTTC CAGAAACCAA AGACTCCACA GGCCCCCAAG 
ACCCTGGCCG GCTCCTGTGG GAAGTTTGCC CCCTTTGAAA TTAAAGAGCA CATGGTCCTG 
GCCCCTCGGC GTCGGACCGC TTTCCATCCA GACCTCTGCA GTCAGCTGGA CCAGCTCCTC 
CAGCAGCAGA GCGGCGAGTT CTCCTTCTTG AAAGACCTCA AAGGCCGGCA GCCCCTGAGG 
TCCGGACCCA CGCACGTTTC CACCCGGAAT GCAGATATTT TTAACAGTGA TGTCGTCATC 
GTGGAGCGTG GGAAGGGCGA CGGTGTTCCC GAGAGGAGGA AGTTTGGCAG GATGAAGCTC 
CTGCAGTTCT GTGAGAACCA CCGGCCTGCC TACTGGGGTA CCTGGAATAA GAAGACGGCA 
CTCATCCGCG CGCGAGACCC CTGGGCCCAG GACACGAAGC TCCTGGACTA TGAGGTGGAC 
AGTGATGAGG AGTGGGAAGA AGAGGAGCCT GGGGAGTCCC TGTCCCACAG TGAGGGGGAT 
GATGATGACG ACATGGGAGA GGATGAAGAT GAGGACGATG GTTTCTTTGT GCCCCATGGG 
TACCTGTCTG AGGACGAAGG TGTGACAGAG GAGTGTGCCG ACCCTGAGAA CCATAAGGTC 
CGCCAGAAAC TGAAGGCCAA GGAGTGGGAC GAGTTCCTGG CTAAGGGGAA GCGCTTTCGC 
GTCCTGCAAC CTGTGAAGAT CGGCTGCGTG TGGGCGGCTG ACAGAGACTG CGCAGGCGAT 
GACCTGAAGG TACTGCAGCA GTTCGCAGCC TGCTTCCTGG AGACCCTGCC GGCCCAGGAG 
GAGCAGACGC CCAAGGCCTC CAAGCGGGAG AGGAGAGACG AGCAGATCCT GGCCCAGCTG 
CTGCCGCTCC TGCACGGCAA TGTGAACGGG AGCAAGGTCA TCATCCGGGA GTTCCAGGAG 
CACTGCCGCC GGGGACTGCT CAGCAACCAC ACCGGCAGCC CGCGGAGCCC CTCCACCACC 
TACCTGCACA CCCCCACCCC CAGCGAGGAT GCCGCCATCC CCTCTAAGTC CCGGCTCAAG 
CGGCTCATTT CCGAGAACTC AGTGTATGAG AAGCGGCCTG ACTTCAGGAT GTGCTGGTAC 
GTGCACCCGC AGGTGCTACA GAGCTTCCAG CAGGAGCACC TGCCCGTGCC GTGCCAGTGG 
AGCTATGTGA CATCGGTGCC CTCGGCCCCC AAAGAGGACA GTGGCAGCGT CCCCTCCACG 
GGGCCCAGCC AGGGCACTCC CATCTCGCTG AAGAGGAAGT CAGCGGGCAG CATGTGCATC 
ACCCAATTCA TGAAGAAGCG CAGGCACGAC GGCCAGATTG GTGCTGAAGA CATGGACGGC 
TTCCAGGCAG ACACGGAGGA GGAGGAAGAG GAGGAGGGCG ACTGTATGAT CGTGGATGTC 
CCGGATGCTG CGGAGGTCCA AGCCCCGTGT GGAGCCGCTT CCGGAGCTGG GGGTGGTGTG 
GGGGTGGACA CCGGCAAGGC CACCCTGACC GCGAGCCCAC TGGGTGCATC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011525909.1
CDS	168..2981
Translation	MLEELECGAPGARGAATAMDCKDRPAFPVKKLIQARLPFKRLNLVPKGKADDMSDDQGTSVQSKSPDLEASLDTLENNCHVGSDIDFRPKLVNGKGPLDNFLRNRIETSIGQSTVIIDLTEDSNEQPDSLVDHNKLNSEASPSREAINGQREDTGDQQGLLKAIQNDKLAFPGETLSDIPCKTEEEGVGCGGAGRRGDSQECSPRSCPELTSGPRMCPRKEQDSWSEAGGILFKGKVPMVVLQDILAVRPPQIKSLPATPQGKNMTPESEVLESFPEEDSVLSHSSLSSPSSTSSPEGPPAPPKQHSSTSPFPTSTPLRRDQERLGKQLKLRAEREEKEKLKEEAKRAKEEAKKKKEEEKELKEKERREKREKDEKEKAEKQRLKEERRKERQEALEAKLEEKRKKEEEKRLREEEKRIKAEKAEITRFFQKPKTPQAPKTLAGSCGKFAPFEIKEHMVLAPRRRTAFHPDLCSQLDQLLQQQSGEFSFLKDLKGRQPLRSGPTHVSTRNADIFNSDVVIVERGKGDGVPERRKFGRMKLLQFCENHRPAYWGTWNKKTALIRARDPWAQDTKLLDYEVDSDEEWEEEEPGESLSHSEGDDDDDMGEDEDEDDGFFVPHGYLSEDEGVTEECADPENHKVRQKLKAKEWDEFLAKGKRFRVLQPVKIGCVWAADRDCAGDDLKVLQQFAACFLETLPAQEEQTPKASKRERRDEQILAQLLPLLHGNVNGSKVIIREFQEHCRRGLLSNHTGSPRSPSTTYLHTPTPSEDAAIPSKSRLKRLISENSVYEKRPDFRMCWYVHPQVLQSFQQEHLPVPCQWSYVTSVPSAPKEDSGSVPSTGPSQGTPISLKRKSAGSMCITQFMKKRRHDGQIGAEDMDGFQADTEEEEEEEGDCMIVDVPDAAEVQAPCGAASGAGGGVGVDTGKATLTASPLGAS

Target ORF information:

RefSeq Version	XM_011527607.2
Organism	Homo sapiens(human)
Definition	Homo sapiens chromatin assembly factor 1 subunit A (CHAF1A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527607.2

ORF Insert Sequence:

ATGCTGGAGG AGCTGGAGTG CGGGGCGCCC GGCGCCAGGG GAGCCGCCAC AGCCATGGAT 
TGCAAAGATA GACCAGCTTT TCCAGTTAAG AAGTTAATAC AAGCCCGTCT GCCGTTTAAG 
CGCCTGAATC TTGTCCCAAA GGGGAAAGCC GATGACATGT CAGACGATCA GGGTACTTCT 
GTGCAAAGTA AAAGCCCCGA TTTAGAGGCC TCTTTGGACA CCTTGGAAAA CAACTGTCAT 
GTGGGTTCTG ACATAGACTT TAGACCGAAA CTTGTCAACG GGAAGGGTCC CTTAGATAAC 
TTTTTAAGAA ATAGAATCGA AACCAGTATT GGCCAGAGCA CAGTCATCAT TGATTTGACA 
GAGGACTCGA ATGAGCAGCC AGACAGTCTT GTGGACCACA ATAAACTAAA TTCTGAAGCC 
TCTCCCTCCA GGGAGGCAAT AAATGGCCAG CGAGAAGACA CTGGGGATCA GCAGGGGTTG 
TTGAAGGCCA TTCAGAACGA CAAGTTGGCA TTTCCTGGAG AGACCCTTTC AGACATTCCT 
TGCAAAACAG AGGAGGAGGG TGTTGGCTGT GGAGGTGCAG GGAGGAGAGG CGACTCCCAG 
GAATGTTCGC CACGGAGCTG CCCGGAGCTG ACGAGTGGCC CGAGAATGTG CCCCAGAAAG 
GAGCAGGACA GTTGGAGTGA AGCTGGGGGC ATCCTGTTCA AAGGGAAGGT GCCTATGGTG 
GTCTTGCAGG ACATCTTGGC TGTGAGACCA CCGCAAATCA AGTCCCTTCC AGCCACACCC 
CAAGGCAAGA ACATGACCCC TGAGAGTGAG GTGCTGGAAT CTTTCCCCGA AGAAGACTCT 
GTACTCAGCC ATTCGTCCCT GAGCTCTCCC TCTTCCACCA GCTCGCCCGA GGGGCCGCCT 
GCTCCCCCAA AGCAGCACAG CAGTACCAGT CCCTTCCCCA CCTCCACGCC CCTCCGCAGA 
GATCAGGAGC GTCTGGGCAA GCAGCTCAAG TTACGTGCAG AAAGGGAAGA AAAGGAGAAG 
CTGAAAGAGG AGGCCAAGCG GGCCAAGGAG GAGGCCAAGA AGAAGAAGGA GGAAGAGAAG 
GAGCTTAAGG AAAAGGAGAG GCGGGAGAAG CGGGAGAAGG ATGAGAAGGA GAAGGCGGAG 
AAGCAGCGGC TCAAGGAGGA GCGGCGCAAG GAGAGACAGG AAGCCCTGGA GGCTAAACTT 
GAGGAAAAAA GGAAAAAGGA AGAAGAGAAA CGGTTAAGAG AAGAAGAGAA GCGCATTAAA 
GCAGAGAAGG CCGAAATCAC GAGGTTCTTC CAGAAACCAA AGACTCCACA GGCCCCCAAG 
ACCCTGGCCG GCTCCTGTGG GAAGTTTGCC CCCTTTGAAA TTAAAGAGCA CATGGTCCTG 
GCCCCTCGGC GTCGGACCGC TTTCCATCCA GACCTCTGCA GTCAGCTGGA CCAGCTCCTC 
CAGCAGCAGA GCGGCGAGTT CTCCTTCTTG AAAGACCTCA AAGGCCGGCA GCCCCTGAGG 
TCCGGACCCA CGCACGTTTC CACCCGGAAT GCAGATATTT TTAACAGTGA TGTCGTCATC 
GTGGAGCGTG GGAAGGGCGA CGGTGTTCCC GAGAGGAGGA AGTTTGGCAG GATGAAGCTC 
CTGCAGTTCT GTGAGAACCA CCGGCCTGCC TACTGGGGTA CCTGGAATAA GAAGACGGCA 
CTCATCCGCG CGCGAGACCC CTGGGCCCAG GACACGAAGC TCCTGGACTA TGAGGTGGAC 
AGTGATGAGG AGTGGGAAGA AGAGGAGCCT GGGGAGTCCC TGTCCCACAG TGAGGGGGAT 
GATGATGACG ACATGGGAGA GGATGAAGAT GAGGACGATG GTTTCTTTGT GCCCCATGGG 
TACCTGTCTG AGGACGAAGG TGTGACAGAG GAGTGTGCCG ACCCTGAGAA CCATAAGGTC 
CGCCAGAAAC TGAAGGCCAA GGAGTGGGAC GAGTTCCTGG CTAAGGGGAA GCGCTTTCGC 
GTCCTGCAAC CTGTGAAGAT CGGCTGCGTG TGGGCGGCTG ACAGAGACTG CGCAGGCGAT 
GACCTGAAGG TACTGCAGCA GTTCGCAGCC TGCTTCCTGG AGACCCTGCC GGCCCAGGAG 
GAGCAGACGC CCAAGGCCTC CAAGCGGGAG AGGAGAGACG AGCAGATCCT GGCCCAGCTG 
CTGCCGCTCC TGCACGGCAA TGTGAACGGG AGCAAGGTCA TCATCCGGGA GTTCCAGGAG 
CACTGCCGCC GGGGACTGCT CAGCAACCAC ACCGGCAGCC CGCGGAGCCC CTCCACCACC 
TACCTGCACA CCCCCACCCC CAGCGAGGAT GCCGCCATCC CCTCTAAGTC CCGGCTCAAG 
CGGCTCATTT CCGAGAACTC AGTGTATGAG AAGCGGCCTG ACTTCAGGAT GTGCTGGTAC 
GTGCACCCGC AGGTGCTACA GAGCTTCCAG CAGGAGCACC TGCCCGTGCC GTGCCAGTGG 
AGCTATGTGA CATCGGTGCC CTCGGCCCCC AAAGAGGACA GTGGCAGCGT CCCCTCCACG 
GGGCCCAGCC AGGGCACTCC CATCTCGCTG AAGAGGAAGT CAGCGGGCAG CATGTGCATC 
ACCCAATTCA TGAAGAAGCG CAGGCACGAC GGCCAGATTG GTGCTGAAGA CATGGACGGC 
TTCCAGGCAG ACACGGAGGA GGAGGAAGAG GAGGAGGGCG ACTGTATGAT CGTGGATGTC 
CCGGATGCTG CGGAGGTCCA AGCCCCGTGT GGAGCCGCTT CCGGAGCTGG GGGTGGTGTG 
GGGGTGGACA CCGGCAAGGC CACCCTGACC GCGAGCCCAC TGGGTGCATC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHAF1A ( NM_005483.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005474.2 Homo sapiens chromatin assembly factor 1 subunit A (CHAF1A), mRNA.

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CloneID	OHu16589
Clone ID Related Accession (Same CDS sequence)	NM_005483.3 , NM_005483.2
Accession Version	NM_005483.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Homo sapiens(human)
Product	chromatin assembly factor 1 subunit A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC011498.7, BQ889365.1, BC052620.1, U20979.1, BG775588.1, BM826511.1, H98924.1, BE796132.1 and AA576757.1. On Nov 22, 2018 this sequence version replaced NM_005483.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.121232.1, SRR1660803.107529.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000301280.10/ ENSP00000301280.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGGAGG AGCTGGAGTG CGGGGCGCCC GGCGCCAGGG GAGCCGCCAC AGCCATGGAT 
TGCAAAGATA GACCAGCTTT TCCAGTTAAG AAGTTAATAC AAGCCCGTCT GCCGTTTAAG 
CGCCTGAATC TTGTCCCAAA GGGGAAAGCC GATGACATGT CAGACGATCA GGGTACTTCT 
GTGCAAAGTA AAAGCCCCGA TTTAGAGGCC TCTTTGGACA CCTTGGAAAA CAACTGTCAT 
GTGGGTTCTG ACATAGACTT TAGACCGAAA CTTGTCAACG GGAAGGGTCC CTTAGATAAC 
TTTTTAAGAA ATAGAATCGA AACCAGTATT GGCCAGAGCA CAGTCATCAT TGATTTGACA 
GAGGACTCGA ATGAGCAGCC AGACAGTCTT GTGGACCACA ATAAACTAAA TTCTGAAGCC 
TCTCCCTCCA GGGAGGCAAT AAATGGCCAG CGAGAAGACA CTGGGGATCA GCAGGGGTTG 
TTGAAGGCCA TTCAGAACGA CAAGTTGGCA TTTCCTGGAG AGACCCTTTC AGACATTCCT 
TGCAAAACAG AGGAGGAGGG TGTTGGCTGT GGAGGTGCAG GGAGGAGAGG CGACTCCCAG 
GAATGTTCGC CACGGAGCTG CCCGGAGCTG ACGAGTGGCC CGAGAATGTG CCCCAGAAAG 
GAGCAGGACA GTTGGAGTGA AGCTGGGGGC ATCCTGTTCA AAGGGAAGGT GCCTATGGTG 
GTCTTGCAGG ACATCTTGGC TGTGAGACCA CCGCAAATCA AGTCCCTTCC AGCCACACCC 
CAAGGCAAGA ACATGACCCC TGAGAGTGAG GTGCTGGAAT CTTTCCCCGA AGAAGACTCT 
GTACTCAGCC ATTCGTCCCT GAGCTCTCCC TCTTCCACCA GCTCGCCCGA GGGGCCGCCT 
GCTCCCCCAA AGCAGCACAG CAGTACCAGT CCCTTCCCCA CCTCCACGCC CCTCCGCAGA 
ATAACTAAGA AATTCGTCAA AGGCTCTACA GAGAAGAACA AGCTCAGACT GCAAAGAGAT 
CAGGAGCGTC TGGGCAAGCA GCTCAAGTTA CGTGCAGAAA GGGAAGAAAA GGAGAAGCTG 
AAAGAGGAGG CCAAGCGGGC CAAGGAGGAG GCCAAGAAGA AGAAGGAGGA AGAGAAGGAG 
CTTAAGGAAA AGGAGAGGCG GGAGAAGCGG GAGAAGGATG AGAAGGAGAA GGCGGAGAAG 
CAGCGGCTCA AGGAGGAGCG GCGCAAGGAG AGACAGGAAG CCCTGGAGGC TAAACTTGAG 
GAAAAAAGGA AAAAGGAAGA AGAGAAACGG TTAAGAGAAG AAGAGAAGCG CATTAAAGCA 
GAGAAGGCCG AAATCACGAG GTTCTTCCAG AAACCAAAGA CTCCACAGGC CCCCAAGACC 
CTGGCCGGCT CCTGTGGGAA GTTTGCCCCC TTTGAAATTA AAGAGCACAT GGTCCTGGCC 
CCTCGGCGTC GGACCGCTTT CCATCCAGAC CTCTGCAGTC AGCTGGACCA GCTCCTCCAG 
CAGCAGAGCG GCGAGTTCTC CTTCTTGAAA GACCTCAAAG GCCGGCAGCC CCTGAGGTCC 
GGACCCACGC ACGTTTCCAC CCGGAATGCA GATATTTTTA ACAGTGATGT CGTCATCGTG 
GAGCGTGGGA AGGGCGACGG TGTTCCCGAG AGGAGGAAGT TTGGCAGGAT GAAGCTCCTG 
CAGTTCTGTG AGAACCACCG GCCTGCCTAC TGGGGTACCT GGAATAAGAA GACGGCACTC 
ATCCGCGCGC GAGACCCCTG GGCCCAGGAC ACGAAGCTCC TGGACTATGA GGTGGACAGT 
GATGAGGAGT GGGAAGAAGA GGAGCCTGGG GAGTCCCTGT CCCACAGTGA GGGGGATGAT 
GATGACGACA TGGGAGAGGA TGAAGATGAG GACGATGGTT TCTTTGTGCC CCATGGGTAC 
CTGTCTGAGG ACGAAGGTGT GACAGAGGAG TGTGCCGACC CTGAGAACCA TAAGGTCCGC 
CAGAAACTGA AGGCCAAGGA GTGGGACGAG TTCCTGGCTA AGGGGAAGCG CTTTCGCGTC 
CTGCAACCTG TGAAGATCGG CTGCGTGTGG GCGGCTGACA GAGACTGCGC AGGCGATGAC 
CTGAAGGTAC TGCAGCAGTT CGCAGCCTGC TTCCTGGAGA CCCTGCCGGC CCAGGAGGAG 
CAGACGCCCA AGGCCTCCAA GCGGGAGAGG AGAGACGAGC AGATCCTGGC CCAGCTGCTG 
CCGCTCCTGC ACGGCAATGT GAACGGGAGC AAGGTCATCA TCCGGGAGTT CCAGGAGCAC 
TGCCGCCGGG GACTGCTCAG CAACCACACC GGCAGCCCGC GGAGCCCCTC CACCACCTAC 
CTGCACACCC CCACCCCCAG CGAGGATGCC GCCATCCCCT CTAAGTCCCG GCTCAAGCGG 
CTCATTTCCG AGAACTCAGT GTATGAGAAG CGGCCTGACT TCAGGATGTG CTGGTACGTG 
CACCCGCAGG TGCTACAGAG CTTCCAGCAG GAGCACCTGC CCGTGCCGTG CCAGTGGAGC 
TATGTGACAT CGGTGCCCTC GGCCCCCAAA GAGGACAGTG GCAGCGTCCC CTCCACGGGG 
CCCAGCCAGG GCACTCCCAT CTCGCTGAAG AGGAAGTCAG CGGGCAGCAT GTGCATCACC 
CAATTCATGA AGAAGCGCAG GCACGACGGC CAGATTGGTG CTGAAGACAT GGACGGCTTC 
CAGGCAGACA CGGAGGAGGA GGAAGAGGAG GAGGGCGACT GTATGATCGT GGATGTCCCG 
GATGCTGCGG AGGTCCAAGC CCCGTGTGGA GCCGCTTCCG GAGCTGGGGG TGGTGTGGGG 
GTGGACACCG GCAAGGCCAC CCTGACCGCG AGCCCACTGG GTGCATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005474.2
CDS	124..2994
Translation	MLEELECGAPGARGAATAMDCKDRPAFPVKKLIQARLPFKRLNLVPKGKADDMSDDQGTSVQSKSPDLEASLDTLENNCHVGSDIDFRPKLVNGKGPLDNFLRNRIETSIGQSTVIIDLTEDSNEQPDSLVDHNKLNSEASPSREAINGQREDTGDQQGLLKAIQNDKLAFPGETLSDIPCKTEEEGVGCGGAGRRGDSQECSPRSCPELTSGPRMCPRKEQDSWSEAGGILFKGKVPMVVLQDILAVRPPQIKSLPATPQGKNMTPESEVLESFPEEDSVLSHSSLSSPSSTSSPEGPPAPPKQHSSTSPFPTSTPLRRITKKFVKGSTEKNKLRLQRDQERLGKQLKLRAEREEKEKLKEEAKRAKEEAKKKKEEEKELKEKERREKREKDEKEKAEKQRLKEERRKERQEALEAKLEEKRKKEEEKRLREEEKRIKAEKAEITRFFQKPKTPQAPKTLAGSCGKFAPFEIKEHMVLAPRRRTAFHPDLCSQLDQLLQQQSGEFSFLKDLKGRQPLRSGPTHVSTRNADIFNSDVVIVERGKGDGVPERRKFGRMKLLQFCENHRPAYWGTWNKKTALIRARDPWAQDTKLLDYEVDSDEEWEEEEPGESLSHSEGDDDDDMGEDEDEDDGFFVPHGYLSEDEGVTEECADPENHKVRQKLKAKEWDEFLAKGKRFRVLQPVKIGCVWAADRDCAGDDLKVLQQFAACFLETLPAQEEQTPKASKRERRDEQILAQLLPLLHGNVNGSKVIIREFQEHCRRGLLSNHTGSPRSPSTTYLHTPTPSEDAAIPSKSRLKRLISENSVYEKRPDFRMCWYVHPQVLQSFQQEHLPVPCQWSYVTSVPSAPKEDSGSVPSTGPSQGTPISLKRKSAGSMCITQFMKKRRHDGQIGAEDMDGFQADTEEEEEEEGDCMIVDVPDAAEVQAPCGAASGAGGGVGVDTGKATLTASPLGAS

Target ORF information:

RefSeq Version	NM_005483.3
Organism	Homo sapiens(human)
Definition	Homo sapiens chromatin assembly factor 1 subunit A (CHAF1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005483.3

ORF Insert Sequence:

ATGCTGGAGG AGCTGGAGTG CGGGGCGCCC GGCGCCAGGG GAGCCGCCAC AGCCATGGAT 
TGCAAAGATA GACCAGCTTT TCCAGTTAAG AAGTTAATAC AAGCCCGTCT GCCGTTTAAG 
CGCCTGAATC TTGTCCCAAA GGGGAAAGCC GATGACATGT CAGACGATCA GGGTACTTCT 
GTGCAAAGTA AAAGCCCCGA TTTAGAGGCC TCTTTGGACA CCTTGGAAAA CAACTGTCAT 
GTGGGTTCTG ACATAGACTT TAGACCGAAA CTTGTCAACG GGAAGGGTCC CTTAGATAAC 
TTTTTAAGAA ATAGAATCGA AACCAGTATT GGCCAGAGCA CAGTCATCAT TGATTTGACA 
GAGGACTCGA ATGAGCAGCC AGACAGTCTT GTGGACCACA ATAAACTAAA TTCTGAAGCC 
TCTCCCTCCA GGGAGGCAAT AAATGGCCAG CGAGAAGACA CTGGGGATCA GCAGGGGTTG 
TTGAAGGCCA TTCAGAACGA CAAGTTGGCA TTTCCTGGAG AGACCCTTTC AGACATTCCT 
TGCAAAACAG AGGAGGAGGG TGTTGGCTGT GGAGGTGCAG GGAGGAGAGG CGACTCCCAG 
GAATGTTCGC CACGGAGCTG CCCGGAGCTG ACGAGTGGCC CGAGAATGTG CCCCAGAAAG 
GAGCAGGACA GTTGGAGTGA AGCTGGGGGC ATCCTGTTCA AAGGGAAGGT GCCTATGGTG 
GTCTTGCAGG ACATCTTGGC TGTGAGACCA CCGCAAATCA AGTCCCTTCC AGCCACACCC 
CAAGGCAAGA ACATGACCCC TGAGAGTGAG GTGCTGGAAT CTTTCCCCGA AGAAGACTCT 
GTACTCAGCC ATTCGTCCCT GAGCTCTCCC TCTTCCACCA GCTCGCCCGA GGGGCCGCCT 
GCTCCCCCAA AGCAGCACAG CAGTACCAGT CCCTTCCCCA CCTCCACGCC CCTCCGCAGA 
ATAACTAAGA AATTCGTCAA AGGCTCTACA GAGAAGAACA AGCTCAGACT GCAAAGAGAT 
CAGGAGCGTC TGGGCAAGCA GCTCAAGTTA CGTGCAGAAA GGGAAGAAAA GGAGAAGCTG 
AAAGAGGAGG CCAAGCGGGC CAAGGAGGAG GCCAAGAAGA AGAAGGAGGA AGAGAAGGAG 
CTTAAGGAAA AGGAGAGGCG GGAGAAGCGG GAGAAGGATG AGAAGGAGAA GGCGGAGAAG 
CAGCGGCTCA AGGAGGAGCG GCGCAAGGAG AGACAGGAAG CCCTGGAGGC TAAACTTGAG 
GAAAAAAGGA AAAAGGAAGA AGAGAAACGG TTAAGAGAAG AAGAGAAGCG CATTAAAGCA 
GAGAAGGCCG AAATCACGAG GTTCTTCCAG AAACCAAAGA CTCCACAGGC CCCCAAGACC 
CTGGCCGGCT CCTGTGGGAA GTTTGCCCCC TTTGAAATTA AAGAGCACAT GGTCCTGGCC 
CCTCGGCGTC GGACCGCTTT CCATCCAGAC CTCTGCAGTC AGCTGGACCA GCTCCTCCAG 
CAGCAGAGCG GCGAGTTCTC CTTCTTGAAA GACCTCAAAG GCCGGCAGCC CCTGAGGTCC 
GGACCCACGC ACGTTTCCAC CCGGAATGCA GATATTTTTA ACAGTGATGT CGTCATCGTG 
GAGCGTGGGA AGGGCGACGG TGTTCCCGAG AGGAGGAAGT TTGGCAGGAT GAAGCTCCTG 
CAGTTCTGTG AGAACCACCG GCCTGCCTAC TGGGGTACCT GGAATAAGAA GACGGCACTC 
ATCCGCGCGC GAGACCCCTG GGCCCAGGAC ACGAAGCTCC TGGACTATGA GGTGGACAGT 
GATGAGGAGT GGGAAGAAGA GGAGCCTGGG GAGTCCCTGT CCCACAGTGA GGGGGATGAT 
GATGACGACA TGGGAGAGGA TGAAGATGAG GACGATGGTT TCTTTGTGCC CCATGGGTAC 
CTGTCTGAGG ACGAAGGTGT GACAGAGGAG TGTGCCGACC CTGAGAACCA TAAGGTCCGC 
CAGAAACTGA AGGCCAAGGA GTGGGACGAG TTCCTGGCTA AGGGGAAGCG CTTTCGCGTC 
CTGCAACCTG TGAAGATCGG CTGCGTGTGG GCGGCTGACA GAGACTGCGC AGGCGATGAC 
CTGAAGGTAC TGCAGCAGTT CGCAGCCTGC TTCCTGGAGA CCCTGCCGGC CCAGGAGGAG 
CAGACGCCCA AGGCCTCCAA GCGGGAGAGG AGAGACGAGC AGATCCTGGC CCAGCTGCTG 
CCGCTCCTGC ACGGCAATGT GAACGGGAGC AAGGTCATCA TCCGGGAGTT CCAGGAGCAC 
TGCCGCCGGG GACTGCTCAG CAACCACACC GGCAGCCCGC GGAGCCCCTC CACCACCTAC 
CTGCACACCC CCACCCCCAG CGAGGATGCC GCCATCCCCT CTAAGTCCCG GCTCAAGCGG 
CTCATTTCCG AGAACTCAGT GTATGAGAAG CGGCCTGACT TCAGGATGTG CTGGTACGTG 
CACCCGCAGG TGCTACAGAG CTTCCAGCAG GAGCACCTGC CCGTGCCGTG CCAGTGGAGC 
TATGTGACAT CGGTGCCCTC GGCCCCCAAA GAGGACAGTG GCAGCGTCCC CTCCACGGGG 
CCCAGCCAGG GCACTCCCAT CTCGCTGAAG AGGAAGTCAG CGGGCAGCAT GTGCATCACC 
CAATTCATGA AGAAGCGCAG GCACGACGGC CAGATTGGTG CTGAAGACAT GGACGGCTTC 
CAGGCAGACA CGGAGGAGGA GGAAGAGGAG GAGGGCGACT GTATGATCGT GGATGTCCCG 
GATGCTGCGG AGGTCCAAGC CCCGTGTGGA GCCGCTTCCG GAGCTGGGGG TGGTGTGGGG 
GTGGACACCG GCAAGGCCAC CCTGACCGCG AGCCCACTGG GTGCATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHAF1A ( NM_005483.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_005474.2 Homo sapiens chromatin assembly factor 1 subunit A (CHAF1A), mRNA.

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CloneID	OHu16589
Clone ID Related Accession (Same CDS sequence)	NM_005483.3 , NM_005483.2
Accession Version	NM_005483.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Homo sapiens(human)
Product	chromatin assembly factor 1 subunit A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BQ889365.1, BC052620.1, U20979.1, BG775588.1, BM826511.1, H98924.1, BE796132.1 and AA576757.1. On Jul 22, 2004 this sequence version replaced NM_005483.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.121232.1, SRR1660803.107529.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGCTGGAGG AGCTGGAGTG CGGGGCGCCC GGCGCCAGGG GAGCCGCCAC AGCCATGGAT 
TGCAAAGATA GACCAGCTTT TCCAGTTAAG AAGTTAATAC AAGCCCGTCT GCCGTTTAAG 
CGCCTGAATC TTGTCCCAAA GGGGAAAGCC GATGACATGT CAGACGATCA GGGTACTTCT 
GTGCAAAGTA AAAGCCCCGA TTTAGAGGCC TCTTTGGACA CCTTGGAAAA CAACTGTCAT 
GTGGGTTCTG ACATAGACTT TAGACCGAAA CTTGTCAACG GGAAGGGTCC CTTAGATAAC 
TTTTTAAGAA ATAGAATCGA AACCAGTATT GGCCAGAGCA CAGTCATCAT TGATTTGACA 
GAGGACTCGA ATGAGCAGCC AGACAGTCTT GTGGACCACA ATAAACTAAA TTCTGAAGCC 
TCTCCCTCCA GGGAGGCAAT AAATGGCCAG CGAGAAGACA CTGGGGATCA GCAGGGGTTG 
TTGAAGGCCA TTCAGAACGA CAAGTTGGCA TTTCCTGGAG AGACCCTTTC AGACATTCCT 
TGCAAAACAG AGGAGGAGGG TGTTGGCTGT GGAGGTGCAG GGAGGAGAGG CGACTCCCAG 
GAATGTTCGC CACGGAGCTG CCCGGAGCTG ACGAGTGGCC CGAGAATGTG CCCCAGAAAG 
GAGCAGGACA GTTGGAGTGA AGCTGGGGGC ATCCTGTTCA AAGGGAAGGT GCCTATGGTG 
GTCTTGCAGG ACATCTTGGC TGTGAGACCA CCGCAAATCA AGTCCCTTCC AGCCACACCC 
CAAGGCAAGA ACATGACCCC TGAGAGTGAG GTGCTGGAAT CTTTCCCCGA AGAAGACTCT 
GTACTCAGCC ATTCGTCCCT GAGCTCTCCC TCTTCCACCA GCTCGCCCGA GGGGCCGCCT 
GCTCCCCCAA AGCAGCACAG CAGTACCAGT CCCTTCCCCA CCTCCACGCC CCTCCGCAGA 
ATAACTAAGA AATTCGTCAA AGGCTCTACA GAGAAGAACA AGCTCAGACT GCAAAGAGAT 
CAGGAGCGTC TGGGCAAGCA GCTCAAGTTA CGTGCAGAAA GGGAAGAAAA GGAGAAGCTG 
AAAGAGGAGG CCAAGCGGGC CAAGGAGGAG GCCAAGAAGA AGAAGGAGGA AGAGAAGGAG 
CTTAAGGAAA AGGAGAGGCG GGAGAAGCGG GAGAAGGATG AGAAGGAGAA GGCGGAGAAG 
CAGCGGCTCA AGGAGGAGCG GCGCAAGGAG AGACAGGAAG CCCTGGAGGC TAAACTTGAG 
GAAAAAAGGA AAAAGGAAGA AGAGAAACGG TTAAGAGAAG AAGAGAAGCG CATTAAAGCA 
GAGAAGGCCG AAATCACGAG GTTCTTCCAG AAACCAAAGA CTCCACAGGC CCCCAAGACC 
CTGGCCGGCT CCTGTGGGAA GTTTGCCCCC TTTGAAATTA AAGAGCACAT GGTCCTGGCC 
CCTCGGCGTC GGACCGCTTT CCATCCAGAC CTCTGCAGTC AGCTGGACCA GCTCCTCCAG 
CAGCAGAGCG GCGAGTTCTC CTTCTTGAAA GACCTCAAAG GCCGGCAGCC CCTGAGGTCC 
GGACCCACGC ACGTTTCCAC CCGGAATGCA GATATTTTTA ACAGTGATGT CGTCATCGTG 
GAGCGTGGGA AGGGCGACGG TGTTCCCGAG AGGAGGAAGT TTGGCAGGAT GAAGCTCCTG 
CAGTTCTGTG AGAACCACCG GCCTGCCTAC TGGGGTACCT GGAATAAGAA GACGGCACTC 
ATCCGCGCGC GAGACCCCTG GGCCCAGGAC ACGAAGCTCC TGGACTATGA GGTGGACAGT 
GATGAGGAGT GGGAAGAAGA GGAGCCTGGG GAGTCCCTGT CCCACAGTGA GGGGGATGAT 
GATGACGACA TGGGAGAGGA TGAAGATGAG GACGATGGTT TCTTTGTGCC CCATGGGTAC 
CTGTCTGAGG ACGAAGGTGT GACAGAGGAG TGTGCCGACC CTGAGAACCA TAAGGTCCGC 
CAGAAACTGA AGGCCAAGGA GTGGGACGAG TTCCTGGCTA AGGGGAAGCG CTTTCGCGTC 
CTGCAACCTG TGAAGATCGG CTGCGTGTGG GCGGCTGACA GAGACTGCGC AGGCGATGAC 
CTGAAGGTAC TGCAGCAGTT CGCAGCCTGC TTCCTGGAGA CCCTGCCGGC CCAGGAGGAG 
CAGACGCCCA AGGCCTCCAA GCGGGAGAGG AGAGACGAGC AGATCCTGGC CCAGCTGCTG 
CCGCTCCTGC ACGGCAATGT GAACGGGAGC AAGGTCATCA TCCGGGAGTT CCAGGAGCAC 
TGCCGCCGGG GACTGCTCAG CAACCACACC GGCAGCCCGC GGAGCCCCTC CACCACCTAC 
CTGCACACCC CCACCCCCAG CGAGGATGCC GCCATCCCCT CTAAGTCCCG GCTCAAGCGG 
CTCATTTCCG AGAACTCAGT GTATGAGAAG CGGCCTGACT TCAGGATGTG CTGGTACGTG 
CACCCGCAGG TGCTACAGAG CTTCCAGCAG GAGCACCTGC CCGTGCCGTG CCAGTGGAGC 
TATGTGACAT CGGTGCCCTC GGCCCCCAAA GAGGACAGTG GCAGCGTCCC CTCCACGGGG 
CCCAGCCAGG GCACTCCCAT CTCGCTGAAG AGGAAGTCAG CGGGCAGCAT GTGCATCACC 
CAATTCATGA AGAAGCGCAG GCACGACGGC CAGATTGGTG CTGAAGACAT GGACGGCTTC 
CAGGCAGACA CGGAGGAGGA GGAAGAGGAG GAGGGCGACT GTATGATCGT GGATGTCCCG 
GATGCTGCGG AGGTCCAAGC CCCGTGTGGA GCCGCTTCCG GAGCTGGGGG TGGTGTGGGG 
GTGGACACCG GCAAGGCCAC CCTGACCGCG AGCCCACTGG GTGCATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005474.2
CDS	101..2971
Translation	MLEELECGAPGARGAATAMDCKDRPAFPVKKLIQARLPFKRLNLVPKGKADDMSDDQGTSVQSKSPDLEASLDTLENNCHVGSDIDFRPKLVNGKGPLDNFLRNRIETSIGQSTVIIDLTEDSNEQPDSLVDHNKLNSEASPSREAINGQREDTGDQQGLLKAIQNDKLAFPGETLSDIPCKTEEEGVGCGGAGRRGDSQECSPRSCPELTSGPRMCPRKEQDSWSEAGGILFKGKVPMVVLQDILAVRPPQIKSLPATPQGKNMTPESEVLESFPEEDSVLSHSSLSSPSSTSSPEGPPAPPKQHSSTSPFPTSTPLRRITKKFVKGSTEKNKLRLQRDQERLGKQLKLRAEREEKEKLKEEAKRAKEEAKKKKEEEKELKEKERREKREKDEKEKAEKQRLKEERRKERQEALEAKLEEKRKKEEEKRLREEEKRIKAEKAEITRFFQKPKTPQAPKTLAGSCGKFAPFEIKEHMVLAPRRRTAFHPDLCSQLDQLLQQQSGEFSFLKDLKGRQPLRSGPTHVSTRNADIFNSDVVIVERGKGDGVPERRKFGRMKLLQFCENHRPAYWGTWNKKTALIRARDPWAQDTKLLDYEVDSDEEWEEEEPGESLSHSEGDDDDDMGEDEDEDDGFFVPHGYLSEDEGVTEECADPENHKVRQKLKAKEWDEFLAKGKRFRVLQPVKIGCVWAADRDCAGDDLKVLQQFAACFLETLPAQEEQTPKASKRERRDEQILAQLLPLLHGNVNGSKVIIREFQEHCRRGLLSNHTGSPRSPSTTYLHTPTPSEDAAIPSKSRLKRLISENSVYEKRPDFRMCWYVHPQVLQSFQQEHLPVPCQWSYVTSVPSAPKEDSGSVPSTGPSQGTPISLKRKSAGSMCITQFMKKRRHDGQIGAEDMDGFQADTEEEEEEEGDCMIVDVPDAAEVQAPCGAASGAGGGVGVDTGKATLTASPLGAS

Target ORF information:

RefSeq Version	NM_005483.2
Organism	Homo sapiens(human)
Definition	Homo sapiens chromatin assembly factor 1 subunit A (CHAF1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005483.2

ORF Insert Sequence:

ATGCTGGAGG AGCTGGAGTG CGGGGCGCCC GGCGCCAGGG GAGCCGCCAC AGCCATGGAT 
TGCAAAGATA GACCAGCTTT TCCAGTTAAG AAGTTAATAC AAGCCCGTCT GCCGTTTAAG 
CGCCTGAATC TTGTCCCAAA GGGGAAAGCC GATGACATGT CAGACGATCA GGGTACTTCT 
GTGCAAAGTA AAAGCCCCGA TTTAGAGGCC TCTTTGGACA CCTTGGAAAA CAACTGTCAT 
GTGGGTTCTG ACATAGACTT TAGACCGAAA CTTGTCAACG GGAAGGGTCC CTTAGATAAC 
TTTTTAAGAA ATAGAATCGA AACCAGTATT GGCCAGAGCA CAGTCATCAT TGATTTGACA 
GAGGACTCGA ATGAGCAGCC AGACAGTCTT GTGGACCACA ATAAACTAAA TTCTGAAGCC 
TCTCCCTCCA GGGAGGCAAT AAATGGCCAG CGAGAAGACA CTGGGGATCA GCAGGGGTTG 
TTGAAGGCCA TTCAGAACGA CAAGTTGGCA TTTCCTGGAG AGACCCTTTC AGACATTCCT 
TGCAAAACAG AGGAGGAGGG TGTTGGCTGT GGAGGTGCAG GGAGGAGAGG CGACTCCCAG 
GAATGTTCGC CACGGAGCTG CCCGGAGCTG ACGAGTGGCC CGAGAATGTG CCCCAGAAAG 
GAGCAGGACA GTTGGAGTGA AGCTGGGGGC ATCCTGTTCA AAGGGAAGGT GCCTATGGTG 
GTCTTGCAGG ACATCTTGGC TGTGAGACCA CCGCAAATCA AGTCCCTTCC AGCCACACCC 
CAAGGCAAGA ACATGACCCC TGAGAGTGAG GTGCTGGAAT CTTTCCCCGA AGAAGACTCT 
GTACTCAGCC ATTCGTCCCT GAGCTCTCCC TCTTCCACCA GCTCGCCCGA GGGGCCGCCT 
GCTCCCCCAA AGCAGCACAG CAGTACCAGT CCCTTCCCCA CCTCCACGCC CCTCCGCAGA 
ATAACTAAGA AATTCGTCAA AGGCTCTACA GAGAAGAACA AGCTCAGACT GCAAAGAGAT 
CAGGAGCGTC TGGGCAAGCA GCTCAAGTTA CGTGCAGAAA GGGAAGAAAA GGAGAAGCTG 
AAAGAGGAGG CCAAGCGGGC CAAGGAGGAG GCCAAGAAGA AGAAGGAGGA AGAGAAGGAG 
CTTAAGGAAA AGGAGAGGCG GGAGAAGCGG GAGAAGGATG AGAAGGAGAA GGCGGAGAAG 
CAGCGGCTCA AGGAGGAGCG GCGCAAGGAG AGACAGGAAG CCCTGGAGGC TAAACTTGAG 
GAAAAAAGGA AAAAGGAAGA AGAGAAACGG TTAAGAGAAG AAGAGAAGCG CATTAAAGCA 
GAGAAGGCCG AAATCACGAG GTTCTTCCAG AAACCAAAGA CTCCACAGGC CCCCAAGACC 
CTGGCCGGCT CCTGTGGGAA GTTTGCCCCC TTTGAAATTA AAGAGCACAT GGTCCTGGCC 
CCTCGGCGTC GGACCGCTTT CCATCCAGAC CTCTGCAGTC AGCTGGACCA GCTCCTCCAG 
CAGCAGAGCG GCGAGTTCTC CTTCTTGAAA GACCTCAAAG GCCGGCAGCC CCTGAGGTCC 
GGACCCACGC ACGTTTCCAC CCGGAATGCA GATATTTTTA ACAGTGATGT CGTCATCGTG 
GAGCGTGGGA AGGGCGACGG TGTTCCCGAG AGGAGGAAGT TTGGCAGGAT GAAGCTCCTG 
CAGTTCTGTG AGAACCACCG GCCTGCCTAC TGGGGTACCT GGAATAAGAA GACGGCACTC 
ATCCGCGCGC GAGACCCCTG GGCCCAGGAC ACGAAGCTCC TGGACTATGA GGTGGACAGT 
GATGAGGAGT GGGAAGAAGA GGAGCCTGGG GAGTCCCTGT CCCACAGTGA GGGGGATGAT 
GATGACGACA TGGGAGAGGA TGAAGATGAG GACGATGGTT TCTTTGTGCC CCATGGGTAC 
CTGTCTGAGG ACGAAGGTGT GACAGAGGAG TGTGCCGACC CTGAGAACCA TAAGGTCCGC 
CAGAAACTGA AGGCCAAGGA GTGGGACGAG TTCCTGGCTA AGGGGAAGCG CTTTCGCGTC 
CTGCAACCTG TGAAGATCGG CTGCGTGTGG GCGGCTGACA GAGACTGCGC AGGCGATGAC 
CTGAAGGTAC TGCAGCAGTT CGCAGCCTGC TTCCTGGAGA CCCTGCCGGC CCAGGAGGAG 
CAGACGCCCA AGGCCTCCAA GCGGGAGAGG AGAGACGAGC AGATCCTGGC CCAGCTGCTG 
CCGCTCCTGC ACGGCAATGT GAACGGGAGC AAGGTCATCA TCCGGGAGTT CCAGGAGCAC 
TGCCGCCGGG GACTGCTCAG CAACCACACC GGCAGCCCGC GGAGCCCCTC CACCACCTAC 
CTGCACACCC CCACCCCCAG CGAGGATGCC GCCATCCCCT CTAAGTCCCG GCTCAAGCGG 
CTCATTTCCG AGAACTCAGT GTATGAGAAG CGGCCTGACT TCAGGATGTG CTGGTACGTG 
CACCCGCAGG TGCTACAGAG CTTCCAGCAG GAGCACCTGC CCGTGCCGTG CCAGTGGAGC 
TATGTGACAT CGGTGCCCTC GGCCCCCAAA GAGGACAGTG GCAGCGTCCC CTCCACGGGG 
CCCAGCCAGG GCACTCCCAT CTCGCTGAAG AGGAAGTCAG CGGGCAGCAT GTGCATCACC 
CAATTCATGA AGAAGCGCAG GCACGACGGC CAGATTGGTG CTGAAGACAT GGACGGCTTC 
CAGGCAGACA CGGAGGAGGA GGAAGAGGAG GAGGGCGACT GTATGATCGT GGATGTCCCG 
GATGCTGCGG AGGTCCAAGC CCCGTGTGGA GCCGCTTCCG GAGCTGGGGG TGGTGTGGGG 
GTGGACACCG GCAAGGCCAC CCTGACCGCG AGCCCACTGG GTGCATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CHAF1A interacts with TCF4 to promote gastric carcinogenesis via upregulation of c-MYC and CCND1 expression.
EBioMedicine3869-78(2018 Dec)
Zheng L,Liang X,Li S,Li T,Shang W,Ma L,Jia X,Shao W,Sun P,Chen C,Jia J

NuRD and CAF-1-mediated silencing of the D4Z4 array is modulated by DUX4-induced MBD3L proteins.
eLife7(2018 03)
Campbell AE,Shadle SC,Jagannathan S,Lim JW,Resnick R,Tawil R,van der Maarel SM,Tapscott SJ

[Highly expressed CHAF1A and PCNA are positively associated with malignancy of cervical squamous cell carcinoma].
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology33(12)1696-1701(2017 Dec)
Luo Y,Wang Q,Tian P,Jia Y

Emerging roles of the histone chaperone CAF-1 in cellular plasticity.
Current opinion in genetics & development4683-94(2017 Oct)
Cheloufi S,Hochedlinger K

Over-expression of CHAF1A in Epithelial Ovarian Cancer can promote cell proliferation and inhibit cell apoptosis.
Biochemical and biophysical research communications486(1)191-197(2017 04)
Xia D,Yang X,Liu W,Shen F,Pan J,Lin Y,Du N,Sun Y,Xi X

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CHAF1A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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