NCKAP5 gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_015289932.1	XP_015145418.1	nck-associated protein 5 isoform X6
XM_015289933.1	XP_015145419.1	nck-associated protein 5 isoform X6
XM_025152642.1	XP_025008410.1	nck-associated protein 5 isoform X3
XM_015289926.1	XP_015145412.1	nck-associated protein 5 isoform X1
XM_015289927.1	XP_015145413.1	nck-associated protein 5 isoform X2
XM_015289931.1	XP_015145417.1	nck-associated protein 5 isoform X5
XM_015289934.1	XP_015145420.1	nck-associated protein 5 isoform X5
XM_025152643.1	XP_025008411.1	nck-associated protein 5 isoform X4
XM_015289928.1	XP_015145414.1	nck-associated protein 5 isoform X3
XM_015289932.2	XP_015145418.1	nck-associated protein 5 isoform X1
XM_015289936.1	XP_015145422.1	nck-associated protein 5 isoform X9
XM_025152641.1	XP_025008409.1	nck-associated protein 5 isoform X2
XM_025152644.1	XP_025008412.1	nck-associated protein 5 isoform X7
XM_015289933.2	XP_015145419.1	nck-associated protein 5 isoform X1
XM_015289930.1	XP_015145416.1	nck-associated protein 5 isoform X4
XM_015289935.1	XP_015145421.1	nck-associated protein 5 isoform X6
XM_025152645.1	XP_025008413.1	nck-associated protein 5 isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa152166	XM_025152644.1 Latest version!	Gallus gallus NCK associated protein 5 (NCKAP5), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa152167	XM_025152645.1 Latest version!	Gallus gallus NCK associated protein 5 (NCKAP5), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa38642	XM_015289935.1 Latest version!	Gallus gallus NCK associated protein 5 (NCKAP5), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa38643	XM_015289936.1 Latest version!	Gallus gallus NCK-associated protein 5 (NCKAP5), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa152163	XM_025152641.1 Latest version!	Gallus gallus NCK associated protein 5 (NCKAP5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa152164	XM_025152642.1 Latest version!	Gallus gallus NCK associated protein 5 (NCKAP5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa152165	XM_025152643.1 Latest version!	Gallus gallus NCK associated protein 5 (NCKAP5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa38635	XM_015289926.1 Latest version!	Gallus gallus NCK-associated protein 5 (NCKAP5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa38636	XM_015289927.1 Latest version!	Gallus gallus NCK-associated protein 5 (NCKAP5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa38637	XM_015289928.1 Latest version!	Gallus gallus NCK-associated protein 5 (NCKAP5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa38638	XM_015289930.1 Latest version!	Gallus gallus NCK-associated protein 5 (NCKAP5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa38639	XM_015289931.1 Latest version!	Gallus gallus NCK-associated protein 5 (NCKAP5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa38640	XM_015289933.2 Latest version!	Gallus gallus NCK associated protein 5 (NCKAP5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa38640	XM_015289932.2 Latest version!	Gallus gallus NCK associated protein 5 (NCKAP5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa38641	XM_015289934.1 Latest version!	Gallus gallus NCK associated protein 5 (NCKAP5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OGa38640	XM_015289932.1	Gallus gallus NCK-associated protein 5 (NCKAP5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa38640	XM_015289933.1	Gallus gallus NCK-associated protein 5 (NCKAP5), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

NCKAP5 ( XM_025152644.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025008412.1 Gallus gallus NCK associated protein 5 (NCKAP5), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OGa152166
Clone ID Related Accession (Same CDS sequence)	XM_025152644.1
Accession Version	XM_025152644.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	nck-associated protein 5 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAA AGAGACTACT ACAAAAAAGG GAAATTGGGA AAAGACTTTC TCTGGACAGC 
AGCCTTGTGG ACTATATGGA CTCAAACAAA TACATAGAGC ATCTAGTGAC ACAGCTAGAG 
GAACAGCGCT GGAATTTATG GAGGGAGAAG CTGTCTGTGG CTCGGCTGCA GCGAGAAGTT 
GCGCAGAGTA AGAGCGAAGG AACCATGCGC GAGAAACTGA TACATGAGTT GGAGGAAGAA 
AGACATTTAA GACTTGAGAG TGAAAAACGA TTACGGGAGG TAACACTGGA GTCAGAGCGT 
AACAGAGCTC AGATGCGTGG CCTACAAGAG CAGTTTTCCA GAATGGAGGA AACGGTGAGA 
AATCTACTAC AGAGCCAAGG ATCCCCAGGC CAGAATGGAG AAGGAACTGT CAATATCATG 
AAGGCATATC AGGAAAAGCT GTCAGAAGAC AGTAGGAAAT GCAAAGAAGT CATGGAAAAA 
AATCACACAG CAGTAGATGA AGATTCAAGA AATGAAAGCA ACAGCACTGA AGAGGAAAAA 
GAAAAGACAA AATTGCTACT GGAGCGTTTG AAAGCTCTGG AGGCAGAGAA TTCAGCACTG 
GCTCTGGAAA ATGAAAATCA GAGGGAACAG TATGAAAGAT GTCTTGATGA GGTAGCTAAT 
CAGGTCGTCC AGGCTCTACT TACTCAAAAG GATCTGAGGG AGGAATGTAT AAAGCTGAAA 
ACGAGAGTTT TTGATTTGGA GCAACAGAAT CAAACATTAA GTGTGCTTTT CCAGCAGAGA 
GTCAAACCAG CTTCTGACCT CCTCCTTCAG AAACTCCATT CGCGCATCTT AGACCTCTCA 
TCTGGAGATT TGCTGTCAGA AGTAGATCGA AACAGAAGCT CGGTGCCGTC ACGACCTGCT 
GATGCTCAGA TTCACGAATG CCAGCAGAAT GTGAAATCCA CTATACCTGT CTTGAAATGC 
CACAGTCAAT TAAATGTGAC AGGTCCTAGC CGCCTGTATC CCCGGAGCAG CTGCAGTAGC 
AGTGAACTCT CCCTTTCAAG TGCTTGCAGT GAATATTCCA GTGGTTCCTC CTACACTTGG 
AATGATGGGA AGACGTGCAG CAAAAGGTCA TCCGTGAGCT GGGATAAAAG AATAAGCATT 
GGTTCTTCAC TCCCAAGCAA CCTCTCTAGT CCTGCAGATG ATCTGCCTCC AACTCGTATC 
AAGGAAAACC ATATCCTAGA AGGACTGAAG AAATTACAGA GGCAAAAAGT ATTACTTGAA 
TCACCTTCAG TAATACCGAA ATGGGGTTAC AAAGATTGCA TGAACTCCAA TGAAGGAATA 
TATTCCCCTG GAGTTAAATG TGGCAGCCAT CATGAGCAGA CTCATTGTAA GCCAATGGAA 
ATAGGAAGTA GTTGCATTGA ACATAACAAA ACTTTCTCTT ATGATTCAGA CTCACATGAT 
GATGCAGATG ATGATTCTTC ATCTTTATTA TTGCTGCAGG AAGTACCAAG CAAAGACTGC 
AGGATCTATT GTAACAAATT AACTCACAGT GTTTCTGACA GTCTCTTTCA CTGGGATCCT 
AATAGGAAAC ATTTGCCAGA AAGAAGTTCA TATTTTAATT CAAAGGAAAG ACCAGAAAAA 
TTGACCAGTT TTGTCAGTGG ATTTCAGGCA GAAGTAGAAT CGTGCACCAG AACAAAGCTG 
CCTGATTTAC AGTTAGATCA AAGCCATGCT AATATAGGCT GGAAGGACCT TAATTTTCAG 
CTTTCAGATA CTGATGACAA TGAAGTCTTG GATGAATTGC ATATAGAAAG CAGTGATGAG 
AAAAGTCCAT CAGATTTATT AGCAACTCCT TTTACTGAGA AGTACACAAA GAACTCTGAT 
GTGCTCATAG TCACAGAGAA TTCTCAGTTT ATATCTAGTG ACAAAGAGGA AAGACAGACA 
TCTGCTCACT CAGACATTAG GCCAAAGACA TGTAATTTCA TTAAACAACA AAAAGTCATA 
AAAAAGACTT CTTCTGAGGA GTGCATTACA GTGATATTTG ATGCTGAGGA CGGTGAGCCT 
ATTGAATTCA GCTCGCATCA AATTGGAGTT GTCACTGTAA CAAGAAATGA GATATCAGTC 
AACCAACCAG AAAGTAGGTC CAGTGCAGAA TATACTGAAC TTATACCTCA GGGAATAGCT 
AATTTGCAGG CAGGAACAAA TGCTAGAGAC TACAGTGTTT TGGAAGGACC TGAAGACAAA 
ACTAAAAAAC AGACTCTTGA AGATAATACA GGGAATAAAA ATATGGTTGT CACCACAACC 
TGCAGTGAAT CTTCACCCTG TGGAAGAATC ATACTGCAAA ATGCACCACG ACAAAAACTA 
GTGAAGCCAG TGTATGATGT GTCGTACAAG ACCAATTCAA GCTCCATTGT TCATGCAGGA 
GTCAATCAAA AGCCAATCTT GACTAAAATA CCCAGCAGAG GTAAATTTTC TCCACAAAAA 
AACAAGATGA CAGAGAGTGA GGAGGCACCT GTAGCTACTT CAGTGACCCC TGCAACCCTT 
GAAAAATCAC CTGCTCTTCC ACCTGTAAAG CTTTCAAGAC TCAAAAAGGC ACAAAGTCCA 
CCTCGTAATT TTGACTCAAA AGTTGACTTC CAAGTTCCAA AGCCCCCTGC CCACCCCCTG 
CCCAGCTCAA AGCGTCCGAG CAGAGGCAAT GGGACAAAGT ATCCAAAGAG TCAGAGTCCA 
GCATCACCAC TGTTGCCTCA GCACCTTATA GAGCCTACTG ACTATGGAGA ACCTCCAACT 
AGAGACTCTA ATCATGATTT AGCAACTGTA GAAGCCAGGT CGCCATCCCC ACCCCTTCCT 
CCCGGCAGGT CAACGTCCCT ATTGATTCGT CCTGATTATG CTCATTCACA ACCTGCGGCT 
GTGAAGTTAG GAGGAGCTGT TCCAGGTGAA ACAGCAAAAA ATATACTGAA ACCATCACCA 
TTAAAAGGAA CTGCAAACTC TGGCATCCAT AATCAATCTA GTCATGATAT GCAAGCAAAA 
AGTCTATCTT CTGGGGCCAA AATTGAGTTA TCTTACCTTC AAGAAAATTC TGAAGCAATT 
ATGCAAAATA AATGTATATC TCAGCAACCC CATAGTGCAT GCCAAGGACT TTCCAAAGTT 
TCCACAAAGC AAGTCTGCCT GAAAAACACC AATCAGATGG GTCTCCACAG GAACCGTGAG 
TTTCTGAGCA TGGATTCTCA GACAGGCAGG TCCATTCCTC TGGGTCCGTC ACTGGAAACA 
ACTTCACAAG AAGTGCATTC CAGCAAGAAG GATATTTCCA GTGACAGTGG GGTTGATCAG 
CCACAAAAGA TGCCAAACAT TCTCCCTGCA ACTCCTCAAT GTCATACAGC AAGCGTGAGT 
GAGCCCTTGC TATCTCAGGT AAACAATTCT CCATTGTCAA CACTGTTTCA TGGTAGTGAC 
ACTGCAAATG CCACACAAAA CTCTAATGAG AAAGGAGTGA AAACCCGTCT CCCTGTAGGA 
CTGAAATTAT TTGTCAAATC TCCTCAACTT CTCCGAAAGA GCTCCACCGT CCCAGGGAAG 
CAGGAAAAAG ATAGTATAAA TGCAGCTTCC AAAAGCCACG TGAGTACAAG TAAGCACAAG 
CAAAATGAAC GTGTGGCCAC CAAAGGTGCA ATAGATGCTG AATTAAAGGA CTCTAAGTCT 
GATACCGAAA TAAAAGATAA TTTTTCTTTG AGACTTAGTG TGGATACAGC ATCACCTCGT 
TCACATGAAA ATGGCAGCTT GGTGGTAGAC AGAGTAGATG AATTAGAAAG CAAACTAGTT 
AAGAGATCTT TTTCTTCTAG CAACAAGTCA CACTTGAAAC CAGCTCTAGG CATGAATGGA 
GCGAAAGCTC GAAGTCAGAG TTTCAGCGTT CATACAGGAG AAAAGTCATC TACCCTTGTG 
ACAGAAGGTC TTGGAAAAGT ACGGACTCAG ATTATTACAA ACACTTCTGA CAGAGGAAAT 
TCTCTAACAA GGCAGAACTC AGCCATGGAA GGACTTCAAA TCAAGGCCAT TCCTGGGTCA 
GGAGCAACAC AAGAGACTCT TTCAAATACT TCAAAAAACA CAGAAAATGC TTACTCTAGA 
CAAGGTTCTC TTGGAAGTAA TAGCAATAAC CAGCATGGAA GCCCAAGCAA ACTGCCATTC 
AGATCATCTC CAAAAGTAGA CTTATTTCGA AACACTTCAA AATATGATGG AAACAAATCA 
TTTTCTATGA AAGATGCACG GAGTCTATCT GTCCAGAGTG AGAAAAGCAG TGAAAATTTA 
AAACACGAAG CTCTAAATAA AAAGAGTGTT TTGCCAGCAG AATTGGAGTT AGATACATCA 
GCTACTGTAT CTTCCAAACA GATTTCATCA ATTCATAGCT TGGGTGGAAT AAAATGTCCG 
CATAAACTGG AAGCAATAAA ATCACAGAGA CAGCAGATGT CTTTAAAGTT AGCAGAAGCC 
CCTCAGGTTA CTGATGTTTT TAGTTCACCT GCTCTGCAAC CTACAATAGA GGAGAAGGTC 
ATGTTGTGCA TCCAAGAAAA TGTCCAGAAG GGTCAGGAAC AAAACAAATC TCCCACTGTA 
GAGACTAAAC AAAAGACTGG GCCCTCTATA GCTAGTTGGT TTGGCTTTCG AAAGAGTAAG 
CTGCCAGCAC TGAGCAGCAG GAAACCTGAC GTTCCTAAAA CAAAGGTAGA AAAAAAAGAA 
CCAAGAGTTT CAGGATTTGG AATTAAGCAG TCGAAATTAG AGAGAAGAAA AGAAAAAAAG 
CAAACTGAAC AACACTGTGA AGTAGAAAAT GAAATTAACA GGAAAACGGA CAGCAGTGAC 
TTTTTAGATG ATGCTATGGA GTTTAAAATT TTGAAGCCAT CACGAAACAC GTCTGGCAAG 
ATTGGATGTG AACAACTCAG AGGTTCCTCC ATAGCTACAT ATTCATCAAA AGACAGTTTT 
ATGAAGGAAC TTCTAAACAG AGTTGATAAG AAAGCAGCTC AGCAGACAGA AAGTGGATCA 
AATAATGTTT CCTACAGGAG TGTGTCGAAG GGCAGCTCCC AGGGCTCCTC TCTTCACAGC 
AACTCTATCA GCTCTCAAGG AAACCACAAG AAAAACAGCA ACACAAAAGC TGATATGGAA 
ATACAGAATC AGACCCTGAG TCTTGCTGCC AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025008412.1
CDS	291..5426
Translation	MERKRLLQKREIGKRLSLDSSLVDYMDSNKYIEHLVTQLEEQRWNLWREKLSVARLQREVAQSKSEGTMREKLIHELEEERHLRLESEKRLREVTLESERNRAQMRGLQEQFSRMEETVRNLLQSQGSPGQNGEGTVNIMKAYQEKLSEDSRKCKEVMEKNHTAVDEDSRNESNSTEEEKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECIKLKTRVFDLEQQNQTLSVLFQQRVKPASDLLLQKLHSRILDLSSGDLLSEVDRNRSSVPSRPADAQIHECQQNVKSTIPVLKCHSQLNVTGPSRLYPRSSCSSSELSLSSACSEYSSGSSYTWNDGKTCSKRSSVSWDKRISIGSSLPSNLSSPADDLPPTRIKENHILEGLKKLQRQKVLLESPSVIPKWGYKDCMNSNEGIYSPGVKCGSHHEQTHCKPMEIGSSCIEHNKTFSYDSDSHDDADDDSSSLLLLQEVPSKDCRIYCNKLTHSVSDSLFHWDPNRKHLPERSSYFNSKERPEKLTSFVSGFQAEVESCTRTKLPDLQLDQSHANIGWKDLNFQLSDTDDNEVLDELHIESSDEKSPSDLLATPFTEKYTKNSDVLIVTENSQFISSDKEERQTSAHSDIRPKTCNFIKQQKVIKKTSSEECITVIFDAEDGEPIEFSSHQIGVVTVTRNEISVNQPESRSSAEYTELIPQGIANLQAGTNARDYSVLEGPEDKTKKQTLEDNTGNKNMVVTTTCSESSPCGRIILQNAPRQKLVKPVYDVSYKTNSSSIVHAGVNQKPILTKIPSRGKFSPQKNKMTESEEAPVATSVTPATLEKSPALPPVKLSRLKKAQSPPRNFDSKVDFQVPKPPAHPLPSSKRPSRGNGTKYPKSQSPASPLLPQHLIEPTDYGEPPTRDSNHDLATVEARSPSPPLPPGRSTSLLIRPDYAHSQPAAVKLGGAVPGETAKNILKPSPLKGTANSGIHNQSSHDMQAKSLSSGAKIELSYLQENSEAIMQNKCISQQPHSACQGLSKVSTKQVCLKNTNQMGLHRNREFLSMDSQTGRSIPLGPSLETTSQEVHSSKKDISSDSGVDQPQKMPNILPATPQCHTASVSEPLLSQVNNSPLSTLFHGSDTANATQNSNEKGVKTRLPVGLKLFVKSPQLLRKSSTVPGKQEKDSINAASKSHVSTSKHKQNERVATKGAIDAELKDSKSDTEIKDNFSLRLSVDTASPRSHENGSLVVDRVDELESKLVKRSFSSSNKSHLKPALGMNGAKARSQSFSVHTGEKSSTLVTEGLGKVRTQIITNTSDRGNSLTRQNSAMEGLQIKAIPGSGATQETLSNTSKNTENAYSRQGSLGSNSNNQHGSPSKLPFRSSPKVDLFRNTSKYDGNKSFSMKDARSLSVQSEKSSENLKHEALNKKSVLPAELELDTSATVSSKQISSIHSLGGIKCPHKLEAIKSQRQQMSLKLAEAPQVTDVFSSPALQPTIEEKVMLCIQENVQKGQEQNKSPTVETKQKTGPSIASWFGFRKSKLPALSSRKPDVPKTKVEKKEPRVSGFGIKQSKLERRKEKKQTEQHCEVENEINRKTDSSDFLDDAMEFKILKPSRNTSGKIGCEQLRGSSIATYSSKDSFMKELLNRVDKKAAQQTESGSNNVSYRSVSKGSSQGSSLHSNSISSQGNHKKNSNTKADMEIQNQTLSLAAR

Target ORF information:

RefSeq Version	XM_025152644.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NCK associated protein 5 (NCKAP5), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025152644.1

ORF Insert Sequence:

ATGGAGAGAA AGAGACTACT ACAAAAAAGG GAAATTGGGA AAAGACTTTC TCTGGACAGC 
AGCCTTGTGG ACTATATGGA CTCAAACAAA TACATAGAGC ATCTAGTGAC ACAGCTAGAG 
GAACAGCGCT GGAATTTATG GAGGGAGAAG CTGTCTGTGG CTCGGCTGCA GCGAGAAGTT 
GCGCAGAGTA AGAGCGAAGG AACCATGCGC GAGAAACTGA TACATGAGTT GGAGGAAGAA 
AGACATTTAA GACTTGAGAG TGAAAAACGA TTACGGGAGG TAACACTGGA GTCAGAGCGT 
AACAGAGCTC AGATGCGTGG CCTACAAGAG CAGTTTTCCA GAATGGAGGA AACGGTGAGA 
AATCTACTAC AGAGCCAAGG ATCCCCAGGC CAGAATGGAG AAGGAACTGT CAATATCATG 
AAGGCATATC AGGAAAAGCT GTCAGAAGAC AGTAGGAAAT GCAAAGAAGT CATGGAAAAA 
AATCACACAG CAGTAGATGA AGATTCAAGA AATGAAAGCA ACAGCACTGA AGAGGAAAAA 
GAAAAGACAA AATTGCTACT GGAGCGTTTG AAAGCTCTGG AGGCAGAGAA TTCAGCACTG 
GCTCTGGAAA ATGAAAATCA GAGGGAACAG TATGAAAGAT GTCTTGATGA GGTAGCTAAT 
CAGGTCGTCC AGGCTCTACT TACTCAAAAG GATCTGAGGG AGGAATGTAT AAAGCTGAAA 
ACGAGAGTTT TTGATTTGGA GCAACAGAAT CAAACATTAA GTGTGCTTTT CCAGCAGAGA 
GTCAAACCAG CTTCTGACCT CCTCCTTCAG AAACTCCATT CGCGCATCTT AGACCTCTCA 
TCTGGAGATT TGCTGTCAGA AGTAGATCGA AACAGAAGCT CGGTGCCGTC ACGACCTGCT 
GATGCTCAGA TTCACGAATG CCAGCAGAAT GTGAAATCCA CTATACCTGT CTTGAAATGC 
CACAGTCAAT TAAATGTGAC AGGTCCTAGC CGCCTGTATC CCCGGAGCAG CTGCAGTAGC 
AGTGAACTCT CCCTTTCAAG TGCTTGCAGT GAATATTCCA GTGGTTCCTC CTACACTTGG 
AATGATGGGA AGACGTGCAG CAAAAGGTCA TCCGTGAGCT GGGATAAAAG AATAAGCATT 
GGTTCTTCAC TCCCAAGCAA CCTCTCTAGT CCTGCAGATG ATCTGCCTCC AACTCGTATC 
AAGGAAAACC ATATCCTAGA AGGACTGAAG AAATTACAGA GGCAAAAAGT ATTACTTGAA 
TCACCTTCAG TAATACCGAA ATGGGGTTAC AAAGATTGCA TGAACTCCAA TGAAGGAATA 
TATTCCCCTG GAGTTAAATG TGGCAGCCAT CATGAGCAGA CTCATTGTAA GCCAATGGAA 
ATAGGAAGTA GTTGCATTGA ACATAACAAA ACTTTCTCTT ATGATTCAGA CTCACATGAT 
GATGCAGATG ATGATTCTTC ATCTTTATTA TTGCTGCAGG AAGTACCAAG CAAAGACTGC 
AGGATCTATT GTAACAAATT AACTCACAGT GTTTCTGACA GTCTCTTTCA CTGGGATCCT 
AATAGGAAAC ATTTGCCAGA AAGAAGTTCA TATTTTAATT CAAAGGAAAG ACCAGAAAAA 
TTGACCAGTT TTGTCAGTGG ATTTCAGGCA GAAGTAGAAT CGTGCACCAG AACAAAGCTG 
CCTGATTTAC AGTTAGATCA AAGCCATGCT AATATAGGCT GGAAGGACCT TAATTTTCAG 
CTTTCAGATA CTGATGACAA TGAAGTCTTG GATGAATTGC ATATAGAAAG CAGTGATGAG 
AAAAGTCCAT CAGATTTATT AGCAACTCCT TTTACTGAGA AGTACACAAA GAACTCTGAT 
GTGCTCATAG TCACAGAGAA TTCTCAGTTT ATATCTAGTG ACAAAGAGGA AAGACAGACA 
TCTGCTCACT CAGACATTAG GCCAAAGACA TGTAATTTCA TTAAACAACA AAAAGTCATA 
AAAAAGACTT CTTCTGAGGA GTGCATTACA GTGATATTTG ATGCTGAGGA CGGTGAGCCT 
ATTGAATTCA GCTCGCATCA AATTGGAGTT GTCACTGTAA CAAGAAATGA GATATCAGTC 
AACCAACCAG AAAGTAGGTC CAGTGCAGAA TATACTGAAC TTATACCTCA GGGAATAGCT 
AATTTGCAGG CAGGAACAAA TGCTAGAGAC TACAGTGTTT TGGAAGGACC TGAAGACAAA 
ACTAAAAAAC AGACTCTTGA AGATAATACA GGGAATAAAA ATATGGTTGT CACCACAACC 
TGCAGTGAAT CTTCACCCTG TGGAAGAATC ATACTGCAAA ATGCACCACG ACAAAAACTA 
GTGAAGCCAG TGTATGATGT GTCGTACAAG ACCAATTCAA GCTCCATTGT TCATGCAGGA 
GTCAATCAAA AGCCAATCTT GACTAAAATA CCCAGCAGAG GTAAATTTTC TCCACAAAAA 
AACAAGATGA CAGAGAGTGA GGAGGCACCT GTAGCTACTT CAGTGACCCC TGCAACCCTT 
GAAAAATCAC CTGCTCTTCC ACCTGTAAAG CTTTCAAGAC TCAAAAAGGC ACAAAGTCCA 
CCTCGTAATT TTGACTCAAA AGTTGACTTC CAAGTTCCAA AGCCCCCTGC CCACCCCCTG 
CCCAGCTCAA AGCGTCCGAG CAGAGGCAAT GGGACAAAGT ATCCAAAGAG TCAGAGTCCA 
GCATCACCAC TGTTGCCTCA GCACCTTATA GAGCCTACTG ACTATGGAGA ACCTCCAACT 
AGAGACTCTA ATCATGATTT AGCAACTGTA GAAGCCAGGT CGCCATCCCC ACCCCTTCCT 
CCCGGCAGGT CAACGTCCCT ATTGATTCGT CCTGATTATG CTCATTCACA ACCTGCGGCT 
GTGAAGTTAG GAGGAGCTGT TCCAGGTGAA ACAGCAAAAA ATATACTGAA ACCATCACCA 
TTAAAAGGAA CTGCAAACTC TGGCATCCAT AATCAATCTA GTCATGATAT GCAAGCAAAA 
AGTCTATCTT CTGGGGCCAA AATTGAGTTA TCTTACCTTC AAGAAAATTC TGAAGCAATT 
ATGCAAAATA AATGTATATC TCAGCAACCC CATAGTGCAT GCCAAGGACT TTCCAAAGTT 
TCCACAAAGC AAGTCTGCCT GAAAAACACC AATCAGATGG GTCTCCACAG GAACCGTGAG 
TTTCTGAGCA TGGATTCTCA GACAGGCAGG TCCATTCCTC TGGGTCCGTC ACTGGAAACA 
ACTTCACAAG AAGTGCATTC CAGCAAGAAG GATATTTCCA GTGACAGTGG GGTTGATCAG 
CCACAAAAGA TGCCAAACAT TCTCCCTGCA ACTCCTCAAT GTCATACAGC AAGCGTGAGT 
GAGCCCTTGC TATCTCAGGT AAACAATTCT CCATTGTCAA CACTGTTTCA TGGTAGTGAC 
ACTGCAAATG CCACACAAAA CTCTAATGAG AAAGGAGTGA AAACCCGTCT CCCTGTAGGA 
CTGAAATTAT TTGTCAAATC TCCTCAACTT CTCCGAAAGA GCTCCACCGT CCCAGGGAAG 
CAGGAAAAAG ATAGTATAAA TGCAGCTTCC AAAAGCCACG TGAGTACAAG TAAGCACAAG 
CAAAATGAAC GTGTGGCCAC CAAAGGTGCA ATAGATGCTG AATTAAAGGA CTCTAAGTCT 
GATACCGAAA TAAAAGATAA TTTTTCTTTG AGACTTAGTG TGGATACAGC ATCACCTCGT 
TCACATGAAA ATGGCAGCTT GGTGGTAGAC AGAGTAGATG AATTAGAAAG CAAACTAGTT 
AAGAGATCTT TTTCTTCTAG CAACAAGTCA CACTTGAAAC CAGCTCTAGG CATGAATGGA 
GCGAAAGCTC GAAGTCAGAG TTTCAGCGTT CATACAGGAG AAAAGTCATC TACCCTTGTG 
ACAGAAGGTC TTGGAAAAGT ACGGACTCAG ATTATTACAA ACACTTCTGA CAGAGGAAAT 
TCTCTAACAA GGCAGAACTC AGCCATGGAA GGACTTCAAA TCAAGGCCAT TCCTGGGTCA 
GGAGCAACAC AAGAGACTCT TTCAAATACT TCAAAAAACA CAGAAAATGC TTACTCTAGA 
CAAGGTTCTC TTGGAAGTAA TAGCAATAAC CAGCATGGAA GCCCAAGCAA ACTGCCATTC 
AGATCATCTC CAAAAGTAGA CTTATTTCGA AACACTTCAA AATATGATGG AAACAAATCA 
TTTTCTATGA AAGATGCACG GAGTCTATCT GTCCAGAGTG AGAAAAGCAG TGAAAATTTA 
AAACACGAAG CTCTAAATAA AAAGAGTGTT TTGCCAGCAG AATTGGAGTT AGATACATCA 
GCTACTGTAT CTTCCAAACA GATTTCATCA ATTCATAGCT TGGGTGGAAT AAAATGTCCG 
CATAAACTGG AAGCAATAAA ATCACAGAGA CAGCAGATGT CTTTAAAGTT AGCAGAAGCC 
CCTCAGGTTA CTGATGTTTT TAGTTCACCT GCTCTGCAAC CTACAATAGA GGAGAAGGTC 
ATGTTGTGCA TCCAAGAAAA TGTCCAGAAG GGTCAGGAAC AAAACAAATC TCCCACTGTA 
GAGACTAAAC AAAAGACTGG GCCCTCTATA GCTAGTTGGT TTGGCTTTCG AAAGAGTAAG 
CTGCCAGCAC TGAGCAGCAG GAAACCTGAC GTTCCTAAAA CAAAGGTAGA AAAAAAAGAA 
CCAAGAGTTT CAGGATTTGG AATTAAGCAG TCGAAATTAG AGAGAAGAAA AGAAAAAAAG 
CAAACTGAAC AACACTGTGA AGTAGAAAAT GAAATTAACA GGAAAACGGA CAGCAGTGAC 
TTTTTAGATG ATGCTATGGA GTTTAAAATT TTGAAGCCAT CACGAAACAC GTCTGGCAAG 
ATTGGATGTG AACAACTCAG AGGTTCCTCC ATAGCTACAT ATTCATCAAA AGACAGTTTT 
ATGAAGGAAC TTCTAAACAG AGTTGATAAG AAAGCAGCTC AGCAGACAGA AAGTGGATCA 
AATAATGTTT CCTACAGGAG TGTGTCGAAG GGCAGCTCCC AGGGCTCCTC TCTTCACAGC 
AACTCTATCA GCTCTCAAGG AAACCACAAG AAAAACAGCA ACACAAAAGC TGATATGGAA 
ATACAGAATC AGACCCTGAG TCTTGCTGCC AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_025152645.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025008413.1 Gallus gallus NCK associated protein 5 (NCKAP5), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OGa152167
Clone ID Related Accession (Same CDS sequence)	XM_025152645.1
Accession Version	XM_025152645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	nck-associated protein 5 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAA AGAGACTACT ACAAAAAAGG GAAATTGGGA AAAGACTTTC TCTGGACAGC 
AGCCTTGTGG ACTATATGGA CTCAAACAAA TACATAGAGC ATCTAGTGAC ACAGCTAGAG 
GAACAGCGCT GGAATTTATG GAGGGAGAAG CTGTCTGTGG CTCGGCTGCA GCGAGAAGTT 
GCGCAGAGTA AGAGCGAAGG AACCATGCGC GAGAAACTGA TACATGAGTT GGAGGAAGAA 
AGACATTTAA GACTTGAGAG TGAAAAACGA TTACGGGAGG TAACACTGGA GTCAGAGCGT 
AACAGAGCTC AGATGCGTGG CCTACAAGAG CAGTTTTCCA GAATGGAGGA AACGGTGAGA 
AATCTACTAC AGAGCCAAGG ATCCCCAGGC CAGAATGGAG AAGGAACTGT CAATATCATG 
AAGGCATATC AGGAAAAGCT GTCAGAAGAC AGTAGGAAAT GCAAAGAAGT CATGGAAAAA 
AATCACACAG CAGTAGATGA AGATTCAAGA AATGAAAGCA ACAGCACTGA AGAGGAAAAA 
GAAAAGACAA AATTGCTACT GGAGCGTTTG AAAGCTCTGG AGGCAGAGAA TTCAGCACTG 
GCTCTGGAAA ATGAAAATCA GAGGGAACAG TATGAAAGAT GTCTTGATGA GGTAGCTAAT 
CAGGTCGTCC AGGCTCTACT TACTCAAAAG GATCTGAGGG AGGAATGTAT AAAGCTGAAA 
ACGAGAGTTT TTGATTTGGA GCAACAGAAT CAAACATTAA GTGTGCTTTT CCAGCAGAGA 
GTCAAACCAG CTTCTGACCT CCTCCTTCAG AAACTCCATT CGCGCATCTT AGACCTCTCA 
TCTGGAGATT TGCTGTCAGA AGTAGATCGA AACAGAAGCT CGGTGCCGTC ACGACCTGCT 
GATGCTCAGA TTCACGAATG CCAGCAGAAT GTGAAATCCA CTATACCTGT CTTGAAATGC 
CACAGTCAAT TAAATGTGAC AGGTCCTAGC CGCCTGTATC CCCGGAGCAG CTGCAGTAGC 
AGTGAACTCT CCCTTTCAAG TGCTTGCAGT GAATATTCCA GTGGTTCCTC CTACACTTGG 
AATGATGGGA AGACGTGCAG CAAAAGGTCA TCCGTGAGCT GGGATAAAAG AATAAGCATT 
GGTTCTTCAC TCCCAAGCAA CCTCTCTAGT CCTGCAGATG ATCTGCCTCC AACTCGTATC 
AAGGAAAACC ATATCCTAGA AGGACTGAAG AAATTACAGA GGCAAAAAGT ATTACTTGAA 
TCACCTTCAG TAATACCGAA ATGGGGTTAC AAAGATTGCA TGAACTCCAA TGAAGGAATA 
TATTCCCCTG GAGTTAAATG TGGCAGCCAT CATGAGCAGA CTCATTGTAA GCCAATGGAA 
ATAGGAAGTA GTTGCATTGA ACATAACAAA ACTTTCTCTT ATGATTCAGA CTCACATGAT 
GATGCAGATG ATGATTCTTC ATCTTTATTA TTGCTGCAGG AAGTACCAAG CAAAGACTGC 
AGGATCTATT GTAACAAATT AACTCACAGT GTTTCTGACA GTCTCTTTCA CTGGGATCCT 
AATAGGAAAC ATTTGCCAGA AAGAAGTTCA TATTTTAATT CAAAGGAAAG ACCAGAAAAA 
TTGACCAGTT TTGTCAGTGG ATTTCAGGCA GAAGTAGAAT CGTGCACCAG AACAAAGCTG 
CCTGATTTAC AGTTAGATCA AAGCCATGCT AATATAGGCT GGAAGGACCT TAATTTTCAG 
CTTTCAGATA CTGATGACAA TGAAGTCTTG GATGAATTGC ATATAGAAAG CAGTGATGAG 
AAAAGTCCAT CAGATTTATT AGCAACTCCT TTTACTGAGA AGTACACAAA GAACTCTGAT 
GTGCTCATAG TCACAGAGAA TTCTCAGTTT ATATCTAGTG ACAAAGAGGA AAGACAGACA 
TCTGCTCACT CAGACATTAG GCCAAAGACA TGTAATTTCA TTAAACAACA AAAAGTCATA 
AAAAAGACTT CTTCTGAGGA GTGCATTACA GTGATATTTG ATGCTGAGGA CGGTGAGCCT 
ATTGAATTCA GCTCGCATCA AATTGGAGTT GTCACTGTAA CAAGAAATGA GATATCAGTC 
AACCAACCAG AAAGTAGGTC CAGTGCAGAA TATACTGAAC TTATACCTCA GGGAATAGCT 
AATTTGCAGG CAGGAACAAA TGCTAGAGAC TACAGTGTTT TGGAAGGACC TGAAGACAAA 
ACTAAAAAAC AGACTCTTGA AGATAATACA GGGAATAAAA ATATGGTTGT CACCACAACC 
TGCAGTGAAT CTTCACCCTG TGGAAGAATC ATACTGCAAA ATGCACCACG ACAAAAACTA 
GTGAAGCCAG TGTATGATGT GTCGTACAAG ACCAATTCAA GCTCCATTGT TCATGCAGGA 
GTCAATCAAA AGCCAATCTT GACTAAAATA CCCAGCAGAG GTAAATTTTC TCCACAAAAA 
AACAAGATGA CAGAGAGTGA GGAGGCACCT GTAGCTACTT CAGTGACCCC TGCAACCCTT 
GAAAAATCAC CTGCTCTTCC ACCTGTAAAG CTTTCAAGAC TCAAAAAGGC ACAAAGTCCA 
CCTCGTAATT TTGACTCAAA AGTTGACTTC CAAGTTCCAA AGCCCCCTGC CCACCCCCTG 
CCCAGCTCAA AGCGTCCGAG CAGAGGCAAT GGGACAAAGT ATCCAAAGAG TCAGAGTCCA 
GCATCACCAC TGTTGCCTCA GCACCTTATA GAGCCTACTG ACTATGGAGA ACCTCCAACT 
AGAGACTCTA ATCATGATTT AGCAACTGTA GAAGCCAGGT CGCCATCCCC ACCCCTTCCT 
CCCGGCAGGT CAACGTCCCT ATTGATTCGT CCTGATTATG CTCATTCACA ACCTGCGGCT 
GTGAAGTTAG GAGGAGCTGT TCCAGGTGAA ACAGCAAAAA ATATACTGAA ACCATCACCA 
TTAAAAGGAA CTGCAAACTC TGGCATCCAT AATCAATCTA GTCATGATAT GCAAGCAAAA 
AGTCTATCTT CTGGGGCCAA AATTGAGTTA TCTTACCTTC AAGAAAATTC TGAAGCAATT 
ATGCAAAATA AATGTATATC TCAGCAACCC CATAGTGCAT GCCAAGGACT TTCCAAAGTT 
TCCACAAAGC AAGTCTGCCT GAAAAACACC AATCAGATGG GTCTCCACAG GAACCGTGAG 
TTTCTGAGCA TGGATTCTCA GACAGGCAGG TCCATTCCTC TGGGTCCGTC ACTGGAAACA 
ACTTCACAAG AAGTGCATTC CAGCAAGAAG GATATTTCCA GTGACAGTGG GGTTGATCAG 
CCACAAAAGA TGCCAAACAT TCTCCCTGCA ACTCCTCAAT GTCATACAGC AAGCGTGAGT 
GAGCCCTTGC TATCTCAGGT AAACAATTCT CCATTGTCAA CACTGTTTCA TGGTAGTGAC 
ACTGCAAATG CCACACAAAA CTCTAATGAG AAAGGAGTGA AAACCCGTCT CCCTGTAGGA 
CTGAAATTAT TTGTCAAATC TCCTCAACTT CTCCGAAAGA GCTCCACCGT CCCAGGGAAG 
CAGGAAAAAG ATAGTATAAA TGCAGCTTCC AAAAGCCACG TGAGTACAAG TAAGCACAAG 
CAAAATGAAC GTGTGGCCAC CAAAGGTGCA ATAGATGCTG AATTAAAGGA CTCTAAGTCT 
GATACCGAAA TAAAAGATAA TTTTTCTTTG AGACTTAGTG TGGATACAGC ATCACCTCGT 
TCACATGAAA ATGGCAGCTT GGTGGTAGAC AGAGTAGATG AATTAGAAAG CAAACTAGTT 
AAGAGATCTT TTTCTTCTAG CAACAAGTCA CACTTGAAAC CAGCTCTAGG CATGAATGGA 
GCGAAAGCTC GAAGTCAGAG TTTCAGCGTT CATACAGGAG AAAAGTCATC TACCCTTGTG 
ACAGAAGGTC TTGGAAAAGT ACGGACTCAG ATTATTACAA ACACTTCTGA CAGAGGAAAT 
TCTCTAACAA GGCAGAACTC AGCCATGGAA GGACTTCAAA TCAAGGCCAT TCCTGGGTCA 
GGAGCAACAC AAGAGACTCT TTCAAATACT TCAAAAAACA CAGAAAATGC TTACTCTAGA 
CAAGGTTCTC TTGGAAGTAA TAGCAATAAC CAGCATGGAA GCCCAAGCAA ACTGCCATTC 
AGATCATCTC CAAAAGTAGA CTTATTTCGA AACACTTCAA AATATGATGG AAACAAATCA 
TTTTCTATGA AAGATGCACG GAGTCTATCT GTCCAGAGTG AGAAAAGCAG TGAAAATTTA 
AAACACGAAG CTCTAAATAA AAAGAGTGTT TTGCCAGCAG AATTGGAGTT AGATACATCA 
GCTACTGTAT CTTCCAAACA GATTTCATCA ATTCATAGCT TGGGTGGAAT AAAATGTCCG 
CATAAACTGG AAGCAATAAA ATCACAGAGA CAGCAGATGT CTTTAAAGTT AGCAGAAGCC 
CCTCAGGTTA CTGATGTTTT TAGTTCACCT GCTCTGCAAC CTACAATAGA GGAGAAGGTC 
ATGTTGTGCA TCCAAGAAAA TGTCCAGAAG GGTCAGGAAC AAAACAAATC TCCCACTGTA 
GAGACTAAAC AAAAGACTGG GCCCTCTATA GCTAGTTGGT TTGGCTTTCG AAAGAGTAAG 
CTGCCAGCAC TGAGCAGCAG GAAACCTGAC GTTCCTAAAA CAAAGGTAGA AAAAAAAGAA 
CCAAGAGTTT CAGGATTTGG AATTAAGCAG TCGAAATTAG AGAGAAGAAA AGAAAAAAAG 
CAAACTGAAC AACACTGTGA AGTAGAAAAT GAAATTAACA GGAAAACGGA CAGCAGTGAC 
TTTTTAGATG ATGCTATGGA GTTTAAAATT TTGAAGCCAT CACGAAACAC GTCTGGCAAG 
ATTGGATGTG AACAACTCAG AGGTTCCTCC ATAGCTACAT ATTCATCAAA AGACAGTTTT 
ATGAAGGAAC TTCTAAACAG GAGTGTGTCG AAGGGCAGCT CCCAGGGCTC CTCTCTTCAC 
AGCAACTCTA TCAGCTCTCA AGGAAACCAC AAGAAAAACA GCAACACAAA AGCTGATATG 
GAAATACAGA ATCAGACCCT GAGTCTTGCT GCCAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025008413.1
CDS	290..5368
Translation	MERKRLLQKREIGKRLSLDSSLVDYMDSNKYIEHLVTQLEEQRWNLWREKLSVARLQREVAQSKSEGTMREKLIHELEEERHLRLESEKRLREVTLESERNRAQMRGLQEQFSRMEETVRNLLQSQGSPGQNGEGTVNIMKAYQEKLSEDSRKCKEVMEKNHTAVDEDSRNESNSTEEEKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECIKLKTRVFDLEQQNQTLSVLFQQRVKPASDLLLQKLHSRILDLSSGDLLSEVDRNRSSVPSRPADAQIHECQQNVKSTIPVLKCHSQLNVTGPSRLYPRSSCSSSELSLSSACSEYSSGSSYTWNDGKTCSKRSSVSWDKRISIGSSLPSNLSSPADDLPPTRIKENHILEGLKKLQRQKVLLESPSVIPKWGYKDCMNSNEGIYSPGVKCGSHHEQTHCKPMEIGSSCIEHNKTFSYDSDSHDDADDDSSSLLLLQEVPSKDCRIYCNKLTHSVSDSLFHWDPNRKHLPERSSYFNSKERPEKLTSFVSGFQAEVESCTRTKLPDLQLDQSHANIGWKDLNFQLSDTDDNEVLDELHIESSDEKSPSDLLATPFTEKYTKNSDVLIVTENSQFISSDKEERQTSAHSDIRPKTCNFIKQQKVIKKTSSEECITVIFDAEDGEPIEFSSHQIGVVTVTRNEISVNQPESRSSAEYTELIPQGIANLQAGTNARDYSVLEGPEDKTKKQTLEDNTGNKNMVVTTTCSESSPCGRIILQNAPRQKLVKPVYDVSYKTNSSSIVHAGVNQKPILTKIPSRGKFSPQKNKMTESEEAPVATSVTPATLEKSPALPPVKLSRLKKAQSPPRNFDSKVDFQVPKPPAHPLPSSKRPSRGNGTKYPKSQSPASPLLPQHLIEPTDYGEPPTRDSNHDLATVEARSPSPPLPPGRSTSLLIRPDYAHSQPAAVKLGGAVPGETAKNILKPSPLKGTANSGIHNQSSHDMQAKSLSSGAKIELSYLQENSEAIMQNKCISQQPHSACQGLSKVSTKQVCLKNTNQMGLHRNREFLSMDSQTGRSIPLGPSLETTSQEVHSSKKDISSDSGVDQPQKMPNILPATPQCHTASVSEPLLSQVNNSPLSTLFHGSDTANATQNSNEKGVKTRLPVGLKLFVKSPQLLRKSSTVPGKQEKDSINAASKSHVSTSKHKQNERVATKGAIDAELKDSKSDTEIKDNFSLRLSVDTASPRSHENGSLVVDRVDELESKLVKRSFSSSNKSHLKPALGMNGAKARSQSFSVHTGEKSSTLVTEGLGKVRTQIITNTSDRGNSLTRQNSAMEGLQIKAIPGSGATQETLSNTSKNTENAYSRQGSLGSNSNNQHGSPSKLPFRSSPKVDLFRNTSKYDGNKSFSMKDARSLSVQSEKSSENLKHEALNKKSVLPAELELDTSATVSSKQISSIHSLGGIKCPHKLEAIKSQRQQMSLKLAEAPQVTDVFSSPALQPTIEEKVMLCIQENVQKGQEQNKSPTVETKQKTGPSIASWFGFRKSKLPALSSRKPDVPKTKVEKKEPRVSGFGIKQSKLERRKEKKQTEQHCEVENEINRKTDSSDFLDDAMEFKILKPSRNTSGKIGCEQLRGSSIATYSSKDSFMKELLNRSVSKGSSQGSSLHSNSISSQGNHKKNSNTKADMEIQNQTLSLAAR

Target ORF information:

RefSeq Version	XM_025152645.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NCK associated protein 5 (NCKAP5), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025152645.1

ORF Insert Sequence:

ATGGAGAGAA AGAGACTACT ACAAAAAAGG GAAATTGGGA AAAGACTTTC TCTGGACAGC 
AGCCTTGTGG ACTATATGGA CTCAAACAAA TACATAGAGC ATCTAGTGAC ACAGCTAGAG 
GAACAGCGCT GGAATTTATG GAGGGAGAAG CTGTCTGTGG CTCGGCTGCA GCGAGAAGTT 
GCGCAGAGTA AGAGCGAAGG AACCATGCGC GAGAAACTGA TACATGAGTT GGAGGAAGAA 
AGACATTTAA GACTTGAGAG TGAAAAACGA TTACGGGAGG TAACACTGGA GTCAGAGCGT 
AACAGAGCTC AGATGCGTGG CCTACAAGAG CAGTTTTCCA GAATGGAGGA AACGGTGAGA 
AATCTACTAC AGAGCCAAGG ATCCCCAGGC CAGAATGGAG AAGGAACTGT CAATATCATG 
AAGGCATATC AGGAAAAGCT GTCAGAAGAC AGTAGGAAAT GCAAAGAAGT CATGGAAAAA 
AATCACACAG CAGTAGATGA AGATTCAAGA AATGAAAGCA ACAGCACTGA AGAGGAAAAA 
GAAAAGACAA AATTGCTACT GGAGCGTTTG AAAGCTCTGG AGGCAGAGAA TTCAGCACTG 
GCTCTGGAAA ATGAAAATCA GAGGGAACAG TATGAAAGAT GTCTTGATGA GGTAGCTAAT 
CAGGTCGTCC AGGCTCTACT TACTCAAAAG GATCTGAGGG AGGAATGTAT AAAGCTGAAA 
ACGAGAGTTT TTGATTTGGA GCAACAGAAT CAAACATTAA GTGTGCTTTT CCAGCAGAGA 
GTCAAACCAG CTTCTGACCT CCTCCTTCAG AAACTCCATT CGCGCATCTT AGACCTCTCA 
TCTGGAGATT TGCTGTCAGA AGTAGATCGA AACAGAAGCT CGGTGCCGTC ACGACCTGCT 
GATGCTCAGA TTCACGAATG CCAGCAGAAT GTGAAATCCA CTATACCTGT CTTGAAATGC 
CACAGTCAAT TAAATGTGAC AGGTCCTAGC CGCCTGTATC CCCGGAGCAG CTGCAGTAGC 
AGTGAACTCT CCCTTTCAAG TGCTTGCAGT GAATATTCCA GTGGTTCCTC CTACACTTGG 
AATGATGGGA AGACGTGCAG CAAAAGGTCA TCCGTGAGCT GGGATAAAAG AATAAGCATT 
GGTTCTTCAC TCCCAAGCAA CCTCTCTAGT CCTGCAGATG ATCTGCCTCC AACTCGTATC 
AAGGAAAACC ATATCCTAGA AGGACTGAAG AAATTACAGA GGCAAAAAGT ATTACTTGAA 
TCACCTTCAG TAATACCGAA ATGGGGTTAC AAAGATTGCA TGAACTCCAA TGAAGGAATA 
TATTCCCCTG GAGTTAAATG TGGCAGCCAT CATGAGCAGA CTCATTGTAA GCCAATGGAA 
ATAGGAAGTA GTTGCATTGA ACATAACAAA ACTTTCTCTT ATGATTCAGA CTCACATGAT 
GATGCAGATG ATGATTCTTC ATCTTTATTA TTGCTGCAGG AAGTACCAAG CAAAGACTGC 
AGGATCTATT GTAACAAATT AACTCACAGT GTTTCTGACA GTCTCTTTCA CTGGGATCCT 
AATAGGAAAC ATTTGCCAGA AAGAAGTTCA TATTTTAATT CAAAGGAAAG ACCAGAAAAA 
TTGACCAGTT TTGTCAGTGG ATTTCAGGCA GAAGTAGAAT CGTGCACCAG AACAAAGCTG 
CCTGATTTAC AGTTAGATCA AAGCCATGCT AATATAGGCT GGAAGGACCT TAATTTTCAG 
CTTTCAGATA CTGATGACAA TGAAGTCTTG GATGAATTGC ATATAGAAAG CAGTGATGAG 
AAAAGTCCAT CAGATTTATT AGCAACTCCT TTTACTGAGA AGTACACAAA GAACTCTGAT 
GTGCTCATAG TCACAGAGAA TTCTCAGTTT ATATCTAGTG ACAAAGAGGA AAGACAGACA 
TCTGCTCACT CAGACATTAG GCCAAAGACA TGTAATTTCA TTAAACAACA AAAAGTCATA 
AAAAAGACTT CTTCTGAGGA GTGCATTACA GTGATATTTG ATGCTGAGGA CGGTGAGCCT 
ATTGAATTCA GCTCGCATCA AATTGGAGTT GTCACTGTAA CAAGAAATGA GATATCAGTC 
AACCAACCAG AAAGTAGGTC CAGTGCAGAA TATACTGAAC TTATACCTCA GGGAATAGCT 
AATTTGCAGG CAGGAACAAA TGCTAGAGAC TACAGTGTTT TGGAAGGACC TGAAGACAAA 
ACTAAAAAAC AGACTCTTGA AGATAATACA GGGAATAAAA ATATGGTTGT CACCACAACC 
TGCAGTGAAT CTTCACCCTG TGGAAGAATC ATACTGCAAA ATGCACCACG ACAAAAACTA 
GTGAAGCCAG TGTATGATGT GTCGTACAAG ACCAATTCAA GCTCCATTGT TCATGCAGGA 
GTCAATCAAA AGCCAATCTT GACTAAAATA CCCAGCAGAG GTAAATTTTC TCCACAAAAA 
AACAAGATGA CAGAGAGTGA GGAGGCACCT GTAGCTACTT CAGTGACCCC TGCAACCCTT 
GAAAAATCAC CTGCTCTTCC ACCTGTAAAG CTTTCAAGAC TCAAAAAGGC ACAAAGTCCA 
CCTCGTAATT TTGACTCAAA AGTTGACTTC CAAGTTCCAA AGCCCCCTGC CCACCCCCTG 
CCCAGCTCAA AGCGTCCGAG CAGAGGCAAT GGGACAAAGT ATCCAAAGAG TCAGAGTCCA 
GCATCACCAC TGTTGCCTCA GCACCTTATA GAGCCTACTG ACTATGGAGA ACCTCCAACT 
AGAGACTCTA ATCATGATTT AGCAACTGTA GAAGCCAGGT CGCCATCCCC ACCCCTTCCT 
CCCGGCAGGT CAACGTCCCT ATTGATTCGT CCTGATTATG CTCATTCACA ACCTGCGGCT 
GTGAAGTTAG GAGGAGCTGT TCCAGGTGAA ACAGCAAAAA ATATACTGAA ACCATCACCA 
TTAAAAGGAA CTGCAAACTC TGGCATCCAT AATCAATCTA GTCATGATAT GCAAGCAAAA 
AGTCTATCTT CTGGGGCCAA AATTGAGTTA TCTTACCTTC AAGAAAATTC TGAAGCAATT 
ATGCAAAATA AATGTATATC TCAGCAACCC CATAGTGCAT GCCAAGGACT TTCCAAAGTT 
TCCACAAAGC AAGTCTGCCT GAAAAACACC AATCAGATGG GTCTCCACAG GAACCGTGAG 
TTTCTGAGCA TGGATTCTCA GACAGGCAGG TCCATTCCTC TGGGTCCGTC ACTGGAAACA 
ACTTCACAAG AAGTGCATTC CAGCAAGAAG GATATTTCCA GTGACAGTGG GGTTGATCAG 
CCACAAAAGA TGCCAAACAT TCTCCCTGCA ACTCCTCAAT GTCATACAGC AAGCGTGAGT 
GAGCCCTTGC TATCTCAGGT AAACAATTCT CCATTGTCAA CACTGTTTCA TGGTAGTGAC 
ACTGCAAATG CCACACAAAA CTCTAATGAG AAAGGAGTGA AAACCCGTCT CCCTGTAGGA 
CTGAAATTAT TTGTCAAATC TCCTCAACTT CTCCGAAAGA GCTCCACCGT CCCAGGGAAG 
CAGGAAAAAG ATAGTATAAA TGCAGCTTCC AAAAGCCACG TGAGTACAAG TAAGCACAAG 
CAAAATGAAC GTGTGGCCAC CAAAGGTGCA ATAGATGCTG AATTAAAGGA CTCTAAGTCT 
GATACCGAAA TAAAAGATAA TTTTTCTTTG AGACTTAGTG TGGATACAGC ATCACCTCGT 
TCACATGAAA ATGGCAGCTT GGTGGTAGAC AGAGTAGATG AATTAGAAAG CAAACTAGTT 
AAGAGATCTT TTTCTTCTAG CAACAAGTCA CACTTGAAAC CAGCTCTAGG CATGAATGGA 
GCGAAAGCTC GAAGTCAGAG TTTCAGCGTT CATACAGGAG AAAAGTCATC TACCCTTGTG 
ACAGAAGGTC TTGGAAAAGT ACGGACTCAG ATTATTACAA ACACTTCTGA CAGAGGAAAT 
TCTCTAACAA GGCAGAACTC AGCCATGGAA GGACTTCAAA TCAAGGCCAT TCCTGGGTCA 
GGAGCAACAC AAGAGACTCT TTCAAATACT TCAAAAAACA CAGAAAATGC TTACTCTAGA 
CAAGGTTCTC TTGGAAGTAA TAGCAATAAC CAGCATGGAA GCCCAAGCAA ACTGCCATTC 
AGATCATCTC CAAAAGTAGA CTTATTTCGA AACACTTCAA AATATGATGG AAACAAATCA 
TTTTCTATGA AAGATGCACG GAGTCTATCT GTCCAGAGTG AGAAAAGCAG TGAAAATTTA 
AAACACGAAG CTCTAAATAA AAAGAGTGTT TTGCCAGCAG AATTGGAGTT AGATACATCA 
GCTACTGTAT CTTCCAAACA GATTTCATCA ATTCATAGCT TGGGTGGAAT AAAATGTCCG 
CATAAACTGG AAGCAATAAA ATCACAGAGA CAGCAGATGT CTTTAAAGTT AGCAGAAGCC 
CCTCAGGTTA CTGATGTTTT TAGTTCACCT GCTCTGCAAC CTACAATAGA GGAGAAGGTC 
ATGTTGTGCA TCCAAGAAAA TGTCCAGAAG GGTCAGGAAC AAAACAAATC TCCCACTGTA 
GAGACTAAAC AAAAGACTGG GCCCTCTATA GCTAGTTGGT TTGGCTTTCG AAAGAGTAAG 
CTGCCAGCAC TGAGCAGCAG GAAACCTGAC GTTCCTAAAA CAAAGGTAGA AAAAAAAGAA 
CCAAGAGTTT CAGGATTTGG AATTAAGCAG TCGAAATTAG AGAGAAGAAA AGAAAAAAAG 
CAAACTGAAC AACACTGTGA AGTAGAAAAT GAAATTAACA GGAAAACGGA CAGCAGTGAC 
TTTTTAGATG ATGCTATGGA GTTTAAAATT TTGAAGCCAT CACGAAACAC GTCTGGCAAG 
ATTGGATGTG AACAACTCAG AGGTTCCTCC ATAGCTACAT ATTCATCAAA AGACAGTTTT 
ATGAAGGAAC TTCTAAACAG GAGTGTGTCG AAGGGCAGCT CCCAGGGCTC CTCTCTTCAC 
AGCAACTCTA TCAGCTCTCA AGGAAACCAC AAGAAAAACA GCAACACAAA AGCTGATATG 
GAAATACAGA ATCAGACCCT GAGTCTTGCT GCCAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_015289935.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145421.1 Gallus gallus NCK associated protein 5 (NCKAP5), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OGa38642
Clone ID Related Accession (Same CDS sequence)	XM_015289935.1
Accession Version	XM_015289935.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	nck-associated protein 5 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAAA ATCACACAGC AGTAGATGAA GATTCAAGAA ATGAAAGCAA CAGCACTGAA 
GAGGAAAAAG AAAAGACAAA ATTGCTACTG GAGCGTTTGA AAGCTCTGGA GGCAGAGAAT 
TCAGCACTGG CTCTGGAAAA TGAAAATCAG AGGGAACAGT ATGAAAGATG TCTTGATGAG 
GTAGCTAATC AGGTCGTCCA GGCTCTACTT ACTCAAAAGG ATCTGAGGGA GGAATGTATA 
AAGCTGAAAA CGAGAGTTTT TGATTTGGAG CAACAGAATC AAACATTAAG TGTGCTTTTC 
CAGCAGAGAG TCAAACCAGC TTCTGACCTC CTCCTTCAGA AACTCCATTC GCGCATCTTA 
GACCTCTCAT CTGGAGATTT GCTGTCAGAA GTAGATCGAA ACAGAAGCTC GGTGCCGTCA 
CGACCTGCTG ATGCTCAGAT TCACGAATGC CAGCAGAATG TGAAATCCAC TATACCTGTC 
TTGAAATGCC ACAGTCAATT AAATGTGACA GGTCCTAGCC GCCTGTATCC CCGGAGCAGC 
TGCAGTAGCA GTGAACTCTC CCTTTCAAGT GCTTGCAGTG AATATTCCAG TGGTTCCTCC 
TACACTTGGA ATGATGGGAA GACGTGCAGC AAAAGGTCAT CCGTGAGCTG GGATAAAAGA 
ATAAGCATTG GTTCTTCACT CCCAAGCAAC CTCTCTAGTC CTGCAGATGA TCTGCCTCCA 
ACTCGTATCA AGGAAAACCA TATCCTAGAA GGACTGAAGA AATTACAGAG GCAAAAAGTA 
TTACTTGAAT CACCTTCAGT AATACCGAAA TGGGGTTACA AAGATTGCAT GAACTCCAAT 
GAAGGAATAT ATTCCCCTGG AGTTAAATGT GGCAGCCATC ATGAGCAGAC TCATTGTAAG 
CCAATGGAAA TAGGAAGTAG TTGCATTGAA CATAACAAAA CTTTCTCTTA TGATTCAGAC 
TCACATGATG ATGCAGATGA TGATTCTTCA TCTTTATTAT TGCTGCAGGA AGTACCAAGC 
AAAGACTGCA GGATCTATTG TAACAAATTA ACTCACAGTG TTTCTGACAG TCTCTTTCAC 
TGGGATCCTA ATAGGAAACA TTTGCCAGAA AGAAGTTCAT ATTTTAATTC AAAGGAAAGA 
CCAGAAAAAT TGACCAGTTT TGTCAGTGGA TTTCAGGCAG AAGTAGAATC GTGCACCAGA 
ACAAAGCTGC CTGATTTACA GTTAGATCAA AGCCATGCTA ATATAGGCTG GAAGGACCTT 
AATTTTCAGC TTTCAGATAC TGATGACAAT GAAGTCTTGG ATGAATTGCA TATAGAAAGC 
AGTGATGAGA AAAGTCCATC AGATTTATTA GCAACTCCTT TTACTGAGAA GTACACAAAG 
AACTCTGATG TGCTCATAGT CACAGAGAAT TCTCAGTTTA TATCTAGTGA CAAAGAGGAA 
AGACAGACAT CTGCTCACTC AGACATTAGG CCAAAGACAT GTAATTTCAT TAAACAACAA 
AAAGTCATAA AAAAGACTTC TTCTGAGGAG TGCATTACAG TGATATTTGA TGCTGAGGAC 
GGTGAGCCTA TTGAATTCAG CTCGCATCAA ATTGGAGTTG TCACTGTAAC AAGAAATGAG 
ATATCAGTCA ACCAACCAGA AAGTAGGTCC AGTGCAGAAT ATACTGAACT TATACCTCAG 
GGAATAGCTA ATTTGCAGGC AGGAACAAAT GCTAGAGACT ACAGTGTTTT GGAAGGACCT 
GAAGACAAAA CTAAAAAACA GACTCTTGAA GATAATACAG GGAATAAAAA TATGGTTGTC 
ACCACAACCT GCAGTGAATC TTCACCCTGT GGAAGAATCA TACTGCAAAA TGCACCACGA 
CAAAAACTAG TGAAGCCAGT GTATGATGTG TCGTACAAGA CCAATTCAAG CTCCATTGTT 
CATGCAGGAG TCAATCAAAA GCCAATCTTG ACTAAAATAC CCAGCAGAGG TAAATTTTCT 
CCACAAAAAA ACAAGATGAC AGAGAGTGAG GAGGCACCTG TAGCTACTTC AGTGACCCCT 
GCAACCCTTG AAAAATCACC TGCTCTTCCA CCTGTAAAGC TTTCAAGACT CAAAAAGGCA 
CAAAGTCCAC CTCGTAATTT TGACTCAAAA GTTGACTTCC AAGTTCCAAA GCCCCCTGCC 
CACCCCCTGC CCAGCTCAAA GCGTCCGAGC AGAGGCAATG GGACAAAGTA TCCAAAGAGT 
CAGAGTCCAG CATCACCACT GTTGCCTCAG CACCTTATAG AGCCTACTGA CTATGGAGAA 
CCTCCAACTA GAGACTCTAA TCATGATTTA GCAACTGTAG AAGCCAGGTC GCCATCCCCA 
CCCCTTCCTC CCGGCAGGTC AACGTCCCTA TTGATTCGTC CTGATTATGC TCATTCACAA 
CCTGCGGCTG TGAAGTTAGG AGGAGCTGTT CCAGGTGAAA CAGCAAAAAA TATACTGAAA 
CCATCACCAT TAAAAGGAAC TGCAAACTCT GGCATCCATA ATCAATCTAG TCATGATATG 
CAAGCAAAAA GTCTATCTTC TGGGGCCAAA ATTGAGTTAT CTTACCTTCA AGAAAATTCT 
GAAGCAATTA TGCAAAATAA ATGTATATCT CAGCAACCCC ATAGTGCATG CCAAGGACTT 
TCCAAAGTTT CCACAAAGCA AGTCTGCCTG AAAAACACCA ATCAGATGGG TCTCCACAGG 
AACCGTGAGT TTCTGAGCAT GGATTCTCAG ACAGGCAGGT CCATTCCTCT GGGTCCGTCA 
CTGGAAACAA CTTCACAAGA AGTGCATTCC AGCAAGAAGG ATATTTCCAG TGACAGTGGG 
GTTGATCAGC CACAAAAGAT GCCAAACATT CTCCCTGCAA CTCCTCAATG TCATACAGCA 
AGCGTGAGTG AGCCCTTGCT ATCTCAGGTA AACAATTCTC CATTGTCAAC ACTGTTTCAT 
GGTAGTGACA CTGCAAATGC CACACAAAAC TCTAATGAGA AAGGAGTGAA AACCCGTCTC 
CCTGTAGGAC TGAAATTATT TGTCAAATCT CCTCAACTTC TCCGAAAGAG CTCCACCGTC 
CCAGGGAAGC AGGAAAAAGA TAGTATAAAT GCAGCTTCCA AAAGCCACGT GAGTACAAGT 
AAGCACAAGC AAAATGAACG TGTGGCCACC AAAGGTGCAA TAGATGCTGA ATTAAAGGAC 
TCTAAGTCTG ATACCGAAAT AAAAGATAAT TTTTCTTTGA GACTTAGTGT GGATACAGCA 
TCACCTCGTT CACATGAAAA TGGCAGCTTG GTGGTAGACA GAGTAGATGA ATTAGAAAGC 
AAACTAGTTA AGAGATCTTT TTCTTCTAGC AACAAGTCAC ACTTGAAACC AGCTCTAGGC 
ATGAATGGAG CGAAAGCTCG AAGTCAGAGT TTCAGCGTTC ATACAGGAGA AAAGTCATCT 
ACCCTTGTGA CAGAAGGTCT TGGAAAAGTA CGGACTCAGA TTATTACAAA CACTTCTGAC 
AGAGGAAATT CTCTAACAAG GCAGAACTCA GCCATGGAAG GACTTCAAAT CAAGGCCATT 
CCTGGGTCAG GAGCAACACA AGAGACTCTT TCAAATACTT CAAAAAACAC AGAAAATGCT 
TACTCTAGAC AAGGTTCTCT TGGAAGTAAT AGCAATAACC AGCATGGAAG CCCAAGCAAA 
CTGCCATTCA GATCATCTCC AAAAGTAGAC TTATTTCGAA ACACTTCAAA ATATGATGGA 
AACAAATCAT TTTCTATGAA AGATGCACGG AGTCTATCTG TCCAGAGTGA GAAAAGCAGT 
GAAAATTTAA AACACGAAGC TCTAAATAAA AAGAGTGTTT TGCCAGCAGA ATTGGAGTTA 
GATACATCAG CTACTGTATC TTCCAAACAG ATTTCATCAA TTCATAGCTT GGGTGGAATA 
AAATGTCCGC ATAAACTGGA AGCAATAAAA TCACAGAGAC AGCAGATGTC TTTAAAGTTA 
GCAGAAGCCC CTCAGGTTAC TGATGTTTTT AGTTCACCTG CTCTGCAACC TACAATAGAG 
GAGAAGGTCA TGTTGTGCAT CCAAGAAAAT GTCCAGAAGG GTCAGGAACA AAACAAATCT 
CCCACTGTAG AGACTAAACA AAAGACTGGG CCCTCTATAG CTAGTTGGTT TGGCTTTCGA 
AAGAGTAAGC TGCCAGCACT GAGCAGCAGG AAACCTGACG TTCCTAAAAC AAAGGTAGAA 
AAAAAAGAAC CAAGAGTTTC AGGATTTGGA ATTAAGCAGT CGAAATTAGA GAGAAGAAAA 
GAAAAAAAGC AAACTGAACA ACACTGTGAA GTAGAAAATG AAATTAACAG GAAAACGGAC 
AGCAGTGACT TTTTAGATGA TGCTATGGAG TTTAAAATTT TGAAGCCATC ACGAAACACG 
TCTGGCAAGA TTGGATGTGA ACAACTCAGA GGTTCCTCCA TAGCTACATA TTCATCAAAA 
GACAGTTTTA TGAAGGAACT TCTAAACAGA GTTGATAAGA AAGCAGCTCA GCAGACAGAA 
AGTGGATCAA ATAATGTTTC CTACAGGAGT GTGTCGAAGG GCAGCTCCCA GGGCTCCTCT 
CTTCACAGCA ACTCTATCAG CTCTCAAGGA AACCACAAGA AAAACAGCAA CACAAAAGCT 
GATATGGAAA TACAGAATCA GACCCTGGCT AACGTAGTCG CAGAAAACCT GCAAGAAGAA 
GAGGAGGACA CCATGACAAG GACTACATGT CAGAGTCATG TGATAGAGTC TTGCTGCCAG 
ATGAGGACGT TGGACAGCGG GATTGGAACC TTCCCACTCC CCGACTCAGG GAACCGATCT 
GCGGGGCGAC ACGTGTCTAA GCAGGAATTA ACCTTGGAAA CAGAAGCCCT AGCACCGTCT 
GAGCAAGTTC TTCTTTCAGC TCCTTCAGTA AAAGCCAAAA CTCTTGAGCG AGAAGTGCCT 
TCAACAGCTA CGAGTCAGGA GTCTGTGGAA AGCATGATTA GTCACTCAAC ATCTGATCCA 
GCCATGACTG CCAAAGGTAT ACGACCTTTT CAAAGTCGCC TTCCAAAACC AGATTCCTCA 
GGAATAATTA ACCTTGCAAA GCAAAGTGAA CAAGAACCAA GTTCTGTGAC CTCTACCTCA 
TTACAGCTTA CTGAAGAAAC TGTAGAAAAT AGAAAAGTTC TTTCAGATTG GACCACCAAG 
AAAACTACAA AAGCAAAGGA CAGAACTCTA AGAGTGTGCA CTTACTCTGC CAGCAGCAGT 
GACGCTGAAC CGGAGCCAGA GTATGGAACA AATTATTTTC AAACTGCAGA GGAGATGTTA 
GTAGAGTTAA CAAAGAGCAA CAAACAAGCT GAGCAGGAAA AACAGAAGCA GAGAAAGTCA 
GTTTTGGGGA ACCCGATGTC AATCTTGGAT TTATACCAGC ACAGTCTCTA TGGCCATTTT 
GGAGAGGATG GACCTGAACA ATTAACACAT TTCAGTTTAA TTGAGCAGCT GAGTGAACCT 
TCCGGTAAAG ACAGCAAGGA CAAAGAGAGC CCAAGTAACC TGAAGCAAAC TGAAGAAACA 
AAAGAAGATC CTCAGACTAG ATTATCTAAA ATTTCCCTGG AGTCTCTCAA TAAATTTAAC 
AGCAATAACG TCCTTTTATT AGAAAAGGAG AAGAACTGTC TGAACAAAGC TGAAGGACAA 
AAGGAAGAAA GCGGAAAGAA GGAAGCATCT TCCTTAAACA GCTCCGATAG GCAGGACATG 
GACAATTTGG AGTCCCTGAG CGATTCTTTG TACGACAGCT TCTCCTCTTG TGCCAGCCAA 
GGCTCTAACG ACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145421.1
CDS	582..6359
Translation	MEKNHTAVDEDSRNESNSTEEEKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECIKLKTRVFDLEQQNQTLSVLFQQRVKPASDLLLQKLHSRILDLSSGDLLSEVDRNRSSVPSRPADAQIHECQQNVKSTIPVLKCHSQLNVTGPSRLYPRSSCSSSELSLSSACSEYSSGSSYTWNDGKTCSKRSSVSWDKRISIGSSLPSNLSSPADDLPPTRIKENHILEGLKKLQRQKVLLESPSVIPKWGYKDCMNSNEGIYSPGVKCGSHHEQTHCKPMEIGSSCIEHNKTFSYDSDSHDDADDDSSSLLLLQEVPSKDCRIYCNKLTHSVSDSLFHWDPNRKHLPERSSYFNSKERPEKLTSFVSGFQAEVESCTRTKLPDLQLDQSHANIGWKDLNFQLSDTDDNEVLDELHIESSDEKSPSDLLATPFTEKYTKNSDVLIVTENSQFISSDKEERQTSAHSDIRPKTCNFIKQQKVIKKTSSEECITVIFDAEDGEPIEFSSHQIGVVTVTRNEISVNQPESRSSAEYTELIPQGIANLQAGTNARDYSVLEGPEDKTKKQTLEDNTGNKNMVVTTTCSESSPCGRIILQNAPRQKLVKPVYDVSYKTNSSSIVHAGVNQKPILTKIPSRGKFSPQKNKMTESEEAPVATSVTPATLEKSPALPPVKLSRLKKAQSPPRNFDSKVDFQVPKPPAHPLPSSKRPSRGNGTKYPKSQSPASPLLPQHLIEPTDYGEPPTRDSNHDLATVEARSPSPPLPPGRSTSLLIRPDYAHSQPAAVKLGGAVPGETAKNILKPSPLKGTANSGIHNQSSHDMQAKSLSSGAKIELSYLQENSEAIMQNKCISQQPHSACQGLSKVSTKQVCLKNTNQMGLHRNREFLSMDSQTGRSIPLGPSLETTSQEVHSSKKDISSDSGVDQPQKMPNILPATPQCHTASVSEPLLSQVNNSPLSTLFHGSDTANATQNSNEKGVKTRLPVGLKLFVKSPQLLRKSSTVPGKQEKDSINAASKSHVSTSKHKQNERVATKGAIDAELKDSKSDTEIKDNFSLRLSVDTASPRSHENGSLVVDRVDELESKLVKRSFSSSNKSHLKPALGMNGAKARSQSFSVHTGEKSSTLVTEGLGKVRTQIITNTSDRGNSLTRQNSAMEGLQIKAIPGSGATQETLSNTSKNTENAYSRQGSLGSNSNNQHGSPSKLPFRSSPKVDLFRNTSKYDGNKSFSMKDARSLSVQSEKSSENLKHEALNKKSVLPAELELDTSATVSSKQISSIHSLGGIKCPHKLEAIKSQRQQMSLKLAEAPQVTDVFSSPALQPTIEEKVMLCIQENVQKGQEQNKSPTVETKQKTGPSIASWFGFRKSKLPALSSRKPDVPKTKVEKKEPRVSGFGIKQSKLERRKEKKQTEQHCEVENEINRKTDSSDFLDDAMEFKILKPSRNTSGKIGCEQLRGSSIATYSSKDSFMKELLNRVDKKAAQQTESGSNNVSYRSVSKGSSQGSSLHSNSISSQGNHKKNSNTKADMEIQNQTLANVVAENLQEEEEDTMTRTTCQSHVIESCCQMRTLDSGIGTFPLPDSGNRSAGRHVSKQELTLETEALAPSEQVLLSAPSVKAKTLEREVPSTATSQESVESMISHSTSDPAMTAKGIRPFQSRLPKPDSSGIINLAKQSEQEPSSVTSTSLQLTEETVENRKVLSDWTTKKTTKAKDRTLRVCTYSASSSDAEPEPEYGTNYFQTAEEMLVELTKSNKQAEQEKQKQRKSVLGNPMSILDLYQHSLYGHFGEDGPEQLTHFSLIEQLSEPSGKDSKDKESPSNLKQTEETKEDPQTRLSKISLESLNKFNSNNVLLLEKEKNCLNKAEGQKEESGKKEASSLNSSDRQDMDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_015289935.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NCK associated protein 5 (NCKAP5), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289935.1

ORF Insert Sequence:

ATGGAAAAAA ATCACACAGC AGTAGATGAA GATTCAAGAA ATGAAAGCAA CAGCACTGAA 
GAGGAAAAAG AAAAGACAAA ATTGCTACTG GAGCGTTTGA AAGCTCTGGA GGCAGAGAAT 
TCAGCACTGG CTCTGGAAAA TGAAAATCAG AGGGAACAGT ATGAAAGATG TCTTGATGAG 
GTAGCTAATC AGGTCGTCCA GGCTCTACTT ACTCAAAAGG ATCTGAGGGA GGAATGTATA 
AAGCTGAAAA CGAGAGTTTT TGATTTGGAG CAACAGAATC AAACATTAAG TGTGCTTTTC 
CAGCAGAGAG TCAAACCAGC TTCTGACCTC CTCCTTCAGA AACTCCATTC GCGCATCTTA 
GACCTCTCAT CTGGAGATTT GCTGTCAGAA GTAGATCGAA ACAGAAGCTC GGTGCCGTCA 
CGACCTGCTG ATGCTCAGAT TCACGAATGC CAGCAGAATG TGAAATCCAC TATACCTGTC 
TTGAAATGCC ACAGTCAATT AAATGTGACA GGTCCTAGCC GCCTGTATCC CCGGAGCAGC 
TGCAGTAGCA GTGAACTCTC CCTTTCAAGT GCTTGCAGTG AATATTCCAG TGGTTCCTCC 
TACACTTGGA ATGATGGGAA GACGTGCAGC AAAAGGTCAT CCGTGAGCTG GGATAAAAGA 
ATAAGCATTG GTTCTTCACT CCCAAGCAAC CTCTCTAGTC CTGCAGATGA TCTGCCTCCA 
ACTCGTATCA AGGAAAACCA TATCCTAGAA GGACTGAAGA AATTACAGAG GCAAAAAGTA 
TTACTTGAAT CACCTTCAGT AATACCGAAA TGGGGTTACA AAGATTGCAT GAACTCCAAT 
GAAGGAATAT ATTCCCCTGG AGTTAAATGT GGCAGCCATC ATGAGCAGAC TCATTGTAAG 
CCAATGGAAA TAGGAAGTAG TTGCATTGAA CATAACAAAA CTTTCTCTTA TGATTCAGAC 
TCACATGATG ATGCAGATGA TGATTCTTCA TCTTTATTAT TGCTGCAGGA AGTACCAAGC 
AAAGACTGCA GGATCTATTG TAACAAATTA ACTCACAGTG TTTCTGACAG TCTCTTTCAC 
TGGGATCCTA ATAGGAAACA TTTGCCAGAA AGAAGTTCAT ATTTTAATTC AAAGGAAAGA 
CCAGAAAAAT TGACCAGTTT TGTCAGTGGA TTTCAGGCAG AAGTAGAATC GTGCACCAGA 
ACAAAGCTGC CTGATTTACA GTTAGATCAA AGCCATGCTA ATATAGGCTG GAAGGACCTT 
AATTTTCAGC TTTCAGATAC TGATGACAAT GAAGTCTTGG ATGAATTGCA TATAGAAAGC 
AGTGATGAGA AAAGTCCATC AGATTTATTA GCAACTCCTT TTACTGAGAA GTACACAAAG 
AACTCTGATG TGCTCATAGT CACAGAGAAT TCTCAGTTTA TATCTAGTGA CAAAGAGGAA 
AGACAGACAT CTGCTCACTC AGACATTAGG CCAAAGACAT GTAATTTCAT TAAACAACAA 
AAAGTCATAA AAAAGACTTC TTCTGAGGAG TGCATTACAG TGATATTTGA TGCTGAGGAC 
GGTGAGCCTA TTGAATTCAG CTCGCATCAA ATTGGAGTTG TCACTGTAAC AAGAAATGAG 
ATATCAGTCA ACCAACCAGA AAGTAGGTCC AGTGCAGAAT ATACTGAACT TATACCTCAG 
GGAATAGCTA ATTTGCAGGC AGGAACAAAT GCTAGAGACT ACAGTGTTTT GGAAGGACCT 
GAAGACAAAA CTAAAAAACA GACTCTTGAA GATAATACAG GGAATAAAAA TATGGTTGTC 
ACCACAACCT GCAGTGAATC TTCACCCTGT GGAAGAATCA TACTGCAAAA TGCACCACGA 
CAAAAACTAG TGAAGCCAGT GTATGATGTG TCGTACAAGA CCAATTCAAG CTCCATTGTT 
CATGCAGGAG TCAATCAAAA GCCAATCTTG ACTAAAATAC CCAGCAGAGG TAAATTTTCT 
CCACAAAAAA ACAAGATGAC AGAGAGTGAG GAGGCACCTG TAGCTACTTC AGTGACCCCT 
GCAACCCTTG AAAAATCACC TGCTCTTCCA CCTGTAAAGC TTTCAAGACT CAAAAAGGCA 
CAAAGTCCAC CTCGTAATTT TGACTCAAAA GTTGACTTCC AAGTTCCAAA GCCCCCTGCC 
CACCCCCTGC CCAGCTCAAA GCGTCCGAGC AGAGGCAATG GGACAAAGTA TCCAAAGAGT 
CAGAGTCCAG CATCACCACT GTTGCCTCAG CACCTTATAG AGCCTACTGA CTATGGAGAA 
CCTCCAACTA GAGACTCTAA TCATGATTTA GCAACTGTAG AAGCCAGGTC GCCATCCCCA 
CCCCTTCCTC CCGGCAGGTC AACGTCCCTA TTGATTCGTC CTGATTATGC TCATTCACAA 
CCTGCGGCTG TGAAGTTAGG AGGAGCTGTT CCAGGTGAAA CAGCAAAAAA TATACTGAAA 
CCATCACCAT TAAAAGGAAC TGCAAACTCT GGCATCCATA ATCAATCTAG TCATGATATG 
CAAGCAAAAA GTCTATCTTC TGGGGCCAAA ATTGAGTTAT CTTACCTTCA AGAAAATTCT 
GAAGCAATTA TGCAAAATAA ATGTATATCT CAGCAACCCC ATAGTGCATG CCAAGGACTT 
TCCAAAGTTT CCACAAAGCA AGTCTGCCTG AAAAACACCA ATCAGATGGG TCTCCACAGG 
AACCGTGAGT TTCTGAGCAT GGATTCTCAG ACAGGCAGGT CCATTCCTCT GGGTCCGTCA 
CTGGAAACAA CTTCACAAGA AGTGCATTCC AGCAAGAAGG ATATTTCCAG TGACAGTGGG 
GTTGATCAGC CACAAAAGAT GCCAAACATT CTCCCTGCAA CTCCTCAATG TCATACAGCA 
AGCGTGAGTG AGCCCTTGCT ATCTCAGGTA AACAATTCTC CATTGTCAAC ACTGTTTCAT 
GGTAGTGACA CTGCAAATGC CACACAAAAC TCTAATGAGA AAGGAGTGAA AACCCGTCTC 
CCTGTAGGAC TGAAATTATT TGTCAAATCT CCTCAACTTC TCCGAAAGAG CTCCACCGTC 
CCAGGGAAGC AGGAAAAAGA TAGTATAAAT GCAGCTTCCA AAAGCCACGT GAGTACAAGT 
AAGCACAAGC AAAATGAACG TGTGGCCACC AAAGGTGCAA TAGATGCTGA ATTAAAGGAC 
TCTAAGTCTG ATACCGAAAT AAAAGATAAT TTTTCTTTGA GACTTAGTGT GGATACAGCA 
TCACCTCGTT CACATGAAAA TGGCAGCTTG GTGGTAGACA GAGTAGATGA ATTAGAAAGC 
AAACTAGTTA AGAGATCTTT TTCTTCTAGC AACAAGTCAC ACTTGAAACC AGCTCTAGGC 
ATGAATGGAG CGAAAGCTCG AAGTCAGAGT TTCAGCGTTC ATACAGGAGA AAAGTCATCT 
ACCCTTGTGA CAGAAGGTCT TGGAAAAGTA CGGACTCAGA TTATTACAAA CACTTCTGAC 
AGAGGAAATT CTCTAACAAG GCAGAACTCA GCCATGGAAG GACTTCAAAT CAAGGCCATT 
CCTGGGTCAG GAGCAACACA AGAGACTCTT TCAAATACTT CAAAAAACAC AGAAAATGCT 
TACTCTAGAC AAGGTTCTCT TGGAAGTAAT AGCAATAACC AGCATGGAAG CCCAAGCAAA 
CTGCCATTCA GATCATCTCC AAAAGTAGAC TTATTTCGAA ACACTTCAAA ATATGATGGA 
AACAAATCAT TTTCTATGAA AGATGCACGG AGTCTATCTG TCCAGAGTGA GAAAAGCAGT 
GAAAATTTAA AACACGAAGC TCTAAATAAA AAGAGTGTTT TGCCAGCAGA ATTGGAGTTA 
GATACATCAG CTACTGTATC TTCCAAACAG ATTTCATCAA TTCATAGCTT GGGTGGAATA 
AAATGTCCGC ATAAACTGGA AGCAATAAAA TCACAGAGAC AGCAGATGTC TTTAAAGTTA 
GCAGAAGCCC CTCAGGTTAC TGATGTTTTT AGTTCACCTG CTCTGCAACC TACAATAGAG 
GAGAAGGTCA TGTTGTGCAT CCAAGAAAAT GTCCAGAAGG GTCAGGAACA AAACAAATCT 
CCCACTGTAG AGACTAAACA AAAGACTGGG CCCTCTATAG CTAGTTGGTT TGGCTTTCGA 
AAGAGTAAGC TGCCAGCACT GAGCAGCAGG AAACCTGACG TTCCTAAAAC AAAGGTAGAA 
AAAAAAGAAC CAAGAGTTTC AGGATTTGGA ATTAAGCAGT CGAAATTAGA GAGAAGAAAA 
GAAAAAAAGC AAACTGAACA ACACTGTGAA GTAGAAAATG AAATTAACAG GAAAACGGAC 
AGCAGTGACT TTTTAGATGA TGCTATGGAG TTTAAAATTT TGAAGCCATC ACGAAACACG 
TCTGGCAAGA TTGGATGTGA ACAACTCAGA GGTTCCTCCA TAGCTACATA TTCATCAAAA 
GACAGTTTTA TGAAGGAACT TCTAAACAGA GTTGATAAGA AAGCAGCTCA GCAGACAGAA 
AGTGGATCAA ATAATGTTTC CTACAGGAGT GTGTCGAAGG GCAGCTCCCA GGGCTCCTCT 
CTTCACAGCA ACTCTATCAG CTCTCAAGGA AACCACAAGA AAAACAGCAA CACAAAAGCT 
GATATGGAAA TACAGAATCA GACCCTGGCT AACGTAGTCG CAGAAAACCT GCAAGAAGAA 
GAGGAGGACA CCATGACAAG GACTACATGT CAGAGTCATG TGATAGAGTC TTGCTGCCAG 
ATGAGGACGT TGGACAGCGG GATTGGAACC TTCCCACTCC CCGACTCAGG GAACCGATCT 
GCGGGGCGAC ACGTGTCTAA GCAGGAATTA ACCTTGGAAA CAGAAGCCCT AGCACCGTCT 
GAGCAAGTTC TTCTTTCAGC TCCTTCAGTA AAAGCCAAAA CTCTTGAGCG AGAAGTGCCT 
TCAACAGCTA CGAGTCAGGA GTCTGTGGAA AGCATGATTA GTCACTCAAC ATCTGATCCA 
GCCATGACTG CCAAAGGTAT ACGACCTTTT CAAAGTCGCC TTCCAAAACC AGATTCCTCA 
GGAATAATTA ACCTTGCAAA GCAAAGTGAA CAAGAACCAA GTTCTGTGAC CTCTACCTCA 
TTACAGCTTA CTGAAGAAAC TGTAGAAAAT AGAAAAGTTC TTTCAGATTG GACCACCAAG 
AAAACTACAA AAGCAAAGGA CAGAACTCTA AGAGTGTGCA CTTACTCTGC CAGCAGCAGT 
GACGCTGAAC CGGAGCCAGA GTATGGAACA AATTATTTTC AAACTGCAGA GGAGATGTTA 
GTAGAGTTAA CAAAGAGCAA CAAACAAGCT GAGCAGGAAA AACAGAAGCA GAGAAAGTCA 
GTTTTGGGGA ACCCGATGTC AATCTTGGAT TTATACCAGC ACAGTCTCTA TGGCCATTTT 
GGAGAGGATG GACCTGAACA ATTAACACAT TTCAGTTTAA TTGAGCAGCT GAGTGAACCT 
TCCGGTAAAG ACAGCAAGGA CAAAGAGAGC CCAAGTAACC TGAAGCAAAC TGAAGAAACA 
AAAGAAGATC CTCAGACTAG ATTATCTAAA ATTTCCCTGG AGTCTCTCAA TAAATTTAAC 
AGCAATAACG TCCTTTTATT AGAAAAGGAG AAGAACTGTC TGAACAAAGC TGAAGGACAA 
AAGGAAGAAA GCGGAAAGAA GGAAGCATCT TCCTTAAACA GCTCCGATAG GCAGGACATG 
GACAATTTGG AGTCCCTGAG CGATTCTTTG TACGACAGCT TCTCCTCTTG TGCCAGCCAA 
GGCTCTAACG ACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_015289936.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145422.1 Gallus gallus NCK-associated protein 5 (NCKAP5), transcript variant X10, mRNA.

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CloneID	OGa38643
Clone ID Related Accession (Same CDS sequence)	XM_015289936.1
Accession Version	XM_015289936.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	nck-associated protein 5 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAC AACTCTTTGA CCTCGGGCTG ACGAGGCGGG CTCTCCAGAA GCAATCTTCC 
CAGCACGGCA GGATTTACGA GGCACGCCAG CCGGCCAGAC CCAACGGTGC GCTGCCCCCT 
CAGCAGGCCG CGTCTCCTTC CCACGCTTCC GACGGCCCCG AGGCGGCACC TCCGCTCGGG 
GAGCGCCGCG GGCCGTACTG TGCGGCGCTG CCCCCGGGGG ATTTCGGCGG CGGGAGAGCC 
TCGCCAGGGG CAGCCCCGGC CCGGGACGAG CCGCCGGCCC CGGCCCCGCC GCCGCAGACA 
ATGGCCGCCC CCGCCCGACC CCCCCCCCAA CCCCCTCCCC GCCGCGGCGA GCCCCTGCTG 
CCGCCGCACG GTTGCCGGAG CCATTCAGTC GCGGCCGATC GCGACCGCGG CAGATGGGCA 
GGATCTGACG GCCCGGTGCT CCGCGGCTGC CCGGGGCCGG GGCGGGCGGC TCGGGCGGAG 
CCCCCCCCCC TCGCCCCCGC GCGAAGTGCC GCCCCCGCAT GCCGAGCGTC CCGCGGCCGC 
GGAGGGAGCG GGGCACTCCG CAGCGCGACG AAGCGGAGGA GGCGCGCGGC GGCCGGCGGC 
GGGGGGCAGC CCGGGGGGCG CGGCCCCGCA CCGCTCGTCG AGGCGGCGGC CGGCGCCGCG 
CTCCCCGGGC CCGGCGCGGG CCGCCCCCGG CCGCCGCAGC ATGCCCCGCC GGGCAGCAGG 
GAGAAGCTGT CTGTGGCTCG GCTGCAGCGA GAAGTTGCGC AGAGTAAGAG CGAAGGAACC 
ATGCGCGAGA AACTGATACA TGAGTTGGAG GAAGAAAGAC ATTTAAGACT TGAGAGTGAA 
AAACGATTAC GGGAGGTAAC ACTGGAGTCA GAGCGTAACA GAGCTCAGAT GCGTGGCCTA 
CAAGAGCAGT TTTCCAGAAT GGAGGAAACG GTGAGAAATC TACTACAGAG CCAAGGATCC 
CCAGGCCAGA ATGGAGAAGG AACTGTCAAT ATCATGAAGG CATATCAGGA AAAGCTGTCA 
GAAGACAGTA GGAAATGCAA AGAAGTCATG GAAAAAAATC ACACAGCAGT AGATGAAGAT 
TCAAGAAATG AAAGCAACAG CACTGAAGAG GAAAAAGAAA AGACAAAATT GCTACTGGAG 
CGTTTGAAAG CTCTGGAGGC AGAGAATTCA GCACTGGCTC TGGAAAATGA AAATCAGAGG 
GAACAGTATG AAAGATGTCT TGATGAGGTA GCTAATCAGG TCGTCCAGGC TCTACTTACT 
CAAAAGGATC TGAGGGAGGA ATGTATAAAG CTGAAAACGA GAGTTTTTGA TTTGGAGCAA 
CAGAATCAAA CATTAAGTGT GCTTTTCCAG CAGAGAGTCA AACCAGCTTC TGACCTCCTC 
CTTCAGAAAC TCCATTCGCG CATCTTAGAC CTCTCATCTG GAGATTTGCT GTCAGAAGTA 
GATCGAAACA GAAGCTCGGT GCCGTCACGA CCTGCTGATG CTCAGATTCA CGAATGCCAG 
CAGAATGTGA AATCCACTAT ACCTGTCTTG AAATGCCACA GTCAATTAAA TGTGACAGGT 
CCTAGCCGCC TGTATCCCCG GAGCAGCTGC AGTAGCAGTG AACTCTCCCT TTCAAGTGCT 
TGCAGTGAAT ATTCCAGTGG TTCCTCCTAC ACTTGGAATG ATGGGAAGAC GTGCAGCAAA 
AGGTCATCCG TGAGCTGGGA TAAAAGAATA AGCATTGGTT CTTCACTCCC AAGCAACCTC 
TCTAGTCCTG CAGATGATCT GCCTCCAACT CGTATCAAGG AAAACCATAT CCTAGAAGGA 
CTGAAGAAAT TACAGAGGCA AAAAGTATTA CTTGAATCAC CTTCAGTAAT ACCGAAATGG 
GGTTACAAAG ATTGCATGAA CTCCAATGAA GGAATATATT CCCCTGGAGT TAAATGTGGC 
AGCCATCATG AGCAGACTCA TTGTAAGCCA ATGGAAATAG GAAGTAGTTG CATTGAACAT 
AACAAAACTT TCTCTTATGA TTCAGACTCA CATGATGATG CAGATGATGA TTCTTCATCT 
TTATTATTGC TGCAGGAAGT ACCAAGCAAA GACTGCAGGA TCTATTGTAA CAAATTAACT 
CACAGTGTTT CTGACAGTCT CTTTCACTGG GATCCTAATA GGAAACATTT GCCAGAAAGA 
AGTTCATATT TTAATTCAAA GGAAAGACCA GAAAAATTGA CCAGTTTTGT CAGTGGATTT 
CAGGCAGAAG TAGAATCGTG CACCAGAACA AAGCTGCCTG ATTTACAGTT AGATCAAAGC 
CATGCTAATA TAGGCTGGAA GGACCTTAAT TTTCAGCTTT CAGATACTGA TGACAATGAA 
GTCTTGGATG AATTGCATAT AGAAAGCAGT GATGAGAAAA GTCCATCAGA TTTATTAGCA 
ACTCCTTTTA CTGAGAAGTA CACAAAGAAC TCTGATGTGC TCATAGTCAC AGAGAATTCT 
CAGTTTATAT CTAGTGACAA AGAGGAAAGA CAGACATCTG CTCACTCAGA CATTAGGCCA 
AAGACATGTA ATTTCATTAA ACAACAAAAA GTCATAAAAA AGACTTCTTC TGAGGAGTGC 
ATTACAGTGA TATTTGATGC TGAGGACGGT GAGCCTATTG AATTCAGCTC GCATCAAATT 
GGAGTTGTCA CTGTAACAAG AAATGAGATA TCAGTCAACC AACCAGAAAG TAGGTCCAGT 
GCAGAATATA CTGAACTTAT ACCTCAGGGA ATAGCTAATT TGCAGGCAGG AACAAATGCT 
AGAGACTACA GTGTTTTGGA AGGACCTGAA GACAAAACTA AAAAACAGAC TCTTGAAGAT 
AATACAGGGA ATAAAAATAT GGTTGTCACC ACAACCTGCA GTGAATCTTC ACCCTGTGGA 
AGAATCATAC TGCAAAATGC ACCACGACAA AAACTAGTGA AGCCAGTGTA TGATGTGTCG 
TACAAGACCA ATTCAAGCTC CATTGTTCAT GCAGGAGTCA ATCAAAAGCC AATCTTGACT 
AAAATACCCA GCAGAGGTAA ATTTTCTCCA CAAAAAAACA AGATGACAGA GAGTGAGGAG 
GCACCTGTAG CTACTTCAGT GACCCCTGCA ACCCTTGAAA AATCACCTGC TCTTCCACCT 
GTAAAGCTTT CAAGACTCAA AAAGGCACAA AGTCCACCTC GTAATTTTGA CTCAAAAGTT 
GACTTCCAAG TTCCAAAGCC CCCTGCCCAC CCCCTGCCCA GCTCAAAGCG TCCGAGCAGA 
GGCAATGGGA CAAAGTATCC AAAGAGTCAG AGTCCAGCAT CACCACTGTT GCCTCAGCAC 
CTTATAGAGC CTACTGACTA TGGAGAACCT CCAACTAGAG ACTCTAATCA TGATTTAGCA 
ACTGTAGAAG CCAGGTCGCC ATCCCCACCC CTTCCTCCCG GCAGGTCAAC GTCCCTATTG 
ATTCGTCCTG ATTATGCTCA TTCACAACCT GCGGCTGTGA AGTTAGGAGG AGCTGTTCCA 
GGTGAAACAG CAAAAAATAT ACTGAAACCA TCACCATTAA AAGGAACTGC AAACTCTGGC 
ATCCATAATC AATCTAGTCA TGATATGCAA GCAAAAAGTC TATCTTCTGG GGCCAAAATT 
GAGTTATCTT ACCTTCAAGA AAATTCTGAA GCAATTATGC AAAATAAATG TATATCTCAG 
CAACCCCATA GTGCATGCCA AGGACTTTCC AAAGTTTCCA CAAAGCAAGT CTGCCTGAAA 
AACACCAATC AGATGGGTCT CCACAGGAAC CGTGAGTTTC TGAGCATGGA TTCTCAGACA 
GGCAGGTCCA TTCCTCTGGG TCCGTCACTG GAAACAACTT CACAAGAAGT GCATTCCAGC 
AAGAAGGATA TTTCCAGTGA CAGTGGGGTT GATCAGCCAC AAAAGATGCC AAACATTCTC 
CCTGCAACTC CTCAATGTCA TACAGCAAGC GTGAGTGAGC CCTTGCTATC TCAGGTAAAC 
AATTCTCCAT TGTCAACACT GTTTCATGGT AGTGACACTG CAAATGCCAC ACAAAACTCT 
AATGAGAAAG GAGTGAAAAC CCGTCTCCCT GTAGGACTGA AATTATTTGT CAAATCTCCT 
CAACTTCTCC GAAAGAGCTC CACCGTCCCA GGGAAGCAGG AAAAAGATAG TATAAATGCA 
GCTTCCAAAA GCCACGTGAG TACAAGTAAG CACAAGCAAA ATGAACGTGT GGCCACCAAA 
GGTGCAATAG ATGCTGAATT AAAGGACTCT AAGTCTGATA CCGAAATAAA AGATAATTTT 
TCTTTGAGAC TTAGTGTGGA TACAGCATCA CCTCGTTCAC ATGAAAATGG CAGCTTGGTG 
GTAGACAGAG TAGATGAATT AGAAAGCAAA CTAGTTAAGA GATCTTTTTC TTCTAGCAAC 
AAGTCACACT TGAAACCAGC TCTAGGCATG AATGGAGCGA AAGCTCGAAG TCAGAGTTTC 
AGCGTTCATA CAGGAGAAAA GTCATCTACC CTTGTGACAG AAGGTCTTGG AAAAGTACGG 
ACTCAGATTA TTACAAACAC TTCTGACAGA GGAAATTCTC TAACAAGGCA GAACTCAGCC 
ATGGAAGGAC TTCAAATCAA GGCCATTCCT GGGTCAGGAG CAACACAAGA GACTCTTTCA 
AATACTTCAA AAAACACAGA AAATGCTTAC TCTAGACAAG GTTCTCTTGG AAGTAATAGC 
AATAACCAGC ATGGAAGCCC AAGCAAACTG CCATTCAGAT CATCTCCAAA AGTAGACTTA 
TTTCGAAACA CTTCAAAATA TGATGGAAAC AAATCATTTT CTATGAAAGA TGCACGGAGT 
CTATCTGTCC AGAGTGAGAA AAGCAGTGAA AATTTAAAAC ACGAAGCTCT AAATAAAAAG 
AGTGTTTTGC CAGCAGAATT GGAGTTAGAT ACATCAGCTA CTGTATCTTC CAAACAGATT 
TCATCAATTC ATAGCTTGGG TGGAATAAAA TGTCCGCATA AACTGGAAGC AATAAAATCA 
CAGAGACAGC AGATGTCTTT AAAGTTAGCA GAAGCCCCTC AGGTTACTGA TGTTTTTAGT 
TCACCTGCTC TGCAACCTAC AATAGAGGAG AAGGTCATGT TGTGCATCCA AGAAAATGTC 
CAGAAGGGTC AGGAACAAAA CAAATCTCCC ACTGTAGAGA CTAAACAAAA GACTGGGCCC 
TCTATAGCTA GTTGGTTTGG CTTTCGAAAG AGTAAGCTGC CAGCACTGAG CAGCAGGAAA 
CCTGACGTTC CTAAAACAAA GGTAGAAAAA AAAGAACCAA GAGTTTCAGG ATTTGGAATT 
AAGCAGTCGA AATTAGAGAG AAGAAAAGAA AAAAAGCAAA CTGAACAACA CTGTGAAGTA 
GAAAATGAAA TTAACAGGAA AACGGACAGC AGTGACTTTT TAGATGATGC TATGGAGTTT 
AAAATTTTGA AGCCATCACG AAACACGTCT GGCAAGATTG GATGTGAACA ACTCAGAGGT 
TCCTCCATAG CTACATATTC ATCAAAAGAC AGTTTTATGA AGGAACTTCT AAACAGAGTT 
GATAAGAAAG CAGCTCAGCA GACAGAAAGT GGATCAAATA ATGTTTCCTA CAGGAGTGTG 
TCGAAGGGCA GCTCCCAGGG CTCCTCTCTT CACAGCAACT CTATCAGCTC TCAAGGAAAC 
CACAAGAAAA ACAGCAACAC AAAAGCTGAT ATGGAAATAC AGAATCAGAC CCTGAGTCTT 
GCTGCCAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145422.1
CDS	5371..11082
Translation	MATQLFDLGLTRRALQKQSSQHGRIYEARQPARPNGALPPQQAASPSHASDGPEAAPPLGERRGPYCAALPPGDFGGGRASPGAAPARDEPPAPAPPPQTMAAPARPPPQPPPRRGEPLLPPHGCRSHSVAADRDRGRWAGSDGPVLRGCPGPGRAARAEPPPLAPARSAAPACRASRGRGGSGALRSATKRRRRAAAGGGGQPGGRGPAPLVEAAAGAALPGPGAGRPRPPQHAPPGSREKLSVARLQREVAQSKSEGTMREKLIHELEEERHLRLESEKRLREVTLESERNRAQMRGLQEQFSRMEETVRNLLQSQGSPGQNGEGTVNIMKAYQEKLSEDSRKCKEVMEKNHTAVDEDSRNESNSTEEEKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECIKLKTRVFDLEQQNQTLSVLFQQRVKPASDLLLQKLHSRILDLSSGDLLSEVDRNRSSVPSRPADAQIHECQQNVKSTIPVLKCHSQLNVTGPSRLYPRSSCSSSELSLSSACSEYSSGSSYTWNDGKTCSKRSSVSWDKRISIGSSLPSNLSSPADDLPPTRIKENHILEGLKKLQRQKVLLESPSVIPKWGYKDCMNSNEGIYSPGVKCGSHHEQTHCKPMEIGSSCIEHNKTFSYDSDSHDDADDDSSSLLLLQEVPSKDCRIYCNKLTHSVSDSLFHWDPNRKHLPERSSYFNSKERPEKLTSFVSGFQAEVESCTRTKLPDLQLDQSHANIGWKDLNFQLSDTDDNEVLDELHIESSDEKSPSDLLATPFTEKYTKNSDVLIVTENSQFISSDKEERQTSAHSDIRPKTCNFIKQQKVIKKTSSEECITVIFDAEDGEPIEFSSHQIGVVTVTRNEISVNQPESRSSAEYTELIPQGIANLQAGTNARDYSVLEGPEDKTKKQTLEDNTGNKNMVVTTTCSESSPCGRIILQNAPRQKLVKPVYDVSYKTNSSSIVHAGVNQKPILTKIPSRGKFSPQKNKMTESEEAPVATSVTPATLEKSPALPPVKLSRLKKAQSPPRNFDSKVDFQVPKPPAHPLPSSKRPSRGNGTKYPKSQSPASPLLPQHLIEPTDYGEPPTRDSNHDLATVEARSPSPPLPPGRSTSLLIRPDYAHSQPAAVKLGGAVPGETAKNILKPSPLKGTANSGIHNQSSHDMQAKSLSSGAKIELSYLQENSEAIMQNKCISQQPHSACQGLSKVSTKQVCLKNTNQMGLHRNREFLSMDSQTGRSIPLGPSLETTSQEVHSSKKDISSDSGVDQPQKMPNILPATPQCHTASVSEPLLSQVNNSPLSTLFHGSDTANATQNSNEKGVKTRLPVGLKLFVKSPQLLRKSSTVPGKQEKDSINAASKSHVSTSKHKQNERVATKGAIDAELKDSKSDTEIKDNFSLRLSVDTASPRSHENGSLVVDRVDELESKLVKRSFSSSNKSHLKPALGMNGAKARSQSFSVHTGEKSSTLVTEGLGKVRTQIITNTSDRGNSLTRQNSAMEGLQIKAIPGSGATQETLSNTSKNTENAYSRQGSLGSNSNNQHGSPSKLPFRSSPKVDLFRNTSKYDGNKSFSMKDARSLSVQSEKSSENLKHEALNKKSVLPAELELDTSATVSSKQISSIHSLGGIKCPHKLEAIKSQRQQMSLKLAEAPQVTDVFSSPALQPTIEEKVMLCIQENVQKGQEQNKSPTVETKQKTGPSIASWFGFRKSKLPALSSRKPDVPKTKVEKKEPRVSGFGIKQSKLERRKEKKQTEQHCEVENEINRKTDSSDFLDDAMEFKILKPSRNTSGKIGCEQLRGSSIATYSSKDSFMKELLNRVDKKAAQQTESGSNNVSYRSVSKGSSQGSSLHSNSISSQGNHKKNSNTKADMEIQNQTLSLAAR

Target ORF information:

RefSeq Version	XM_015289936.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NCK-associated protein 5 (NCKAP5), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289936.1

ORF Insert Sequence:

ATGGCCACAC AACTCTTTGA CCTCGGGCTG ACGAGGCGGG CTCTCCAGAA GCAATCTTCC 
CAGCACGGCA GGATTTACGA GGCACGCCAG CCGGCCAGAC CCAACGGTGC GCTGCCCCCT 
CAGCAGGCCG CGTCTCCTTC CCACGCTTCC GACGGCCCCG AGGCGGCACC TCCGCTCGGG 
GAGCGCCGCG GGCCGTACTG TGCGGCGCTG CCCCCGGGGG ATTTCGGCGG CGGGAGAGCC 
TCGCCAGGGG CAGCCCCGGC CCGGGACGAG CCGCCGGCCC CGGCCCCGCC GCCGCAGACA 
ATGGCCGCCC CCGCCCGACC CCCCCCCCAA CCCCCTCCCC GCCGCGGCGA GCCCCTGCTG 
CCGCCGCACG GTTGCCGGAG CCATTCAGTC GCGGCCGATC GCGACCGCGG CAGATGGGCA 
GGATCTGACG GCCCGGTGCT CCGCGGCTGC CCGGGGCCGG GGCGGGCGGC TCGGGCGGAG 
CCCCCCCCCC TCGCCCCCGC GCGAAGTGCC GCCCCCGCAT GCCGAGCGTC CCGCGGCCGC 
GGAGGGAGCG GGGCACTCCG CAGCGCGACG AAGCGGAGGA GGCGCGCGGC GGCCGGCGGC 
GGGGGGCAGC CCGGGGGGCG CGGCCCCGCA CCGCTCGTCG AGGCGGCGGC CGGCGCCGCG 
CTCCCCGGGC CCGGCGCGGG CCGCCCCCGG CCGCCGCAGC ATGCCCCGCC GGGCAGCAGG 
GAGAAGCTGT CTGTGGCTCG GCTGCAGCGA GAAGTTGCGC AGAGTAAGAG CGAAGGAACC 
ATGCGCGAGA AACTGATACA TGAGTTGGAG GAAGAAAGAC ATTTAAGACT TGAGAGTGAA 
AAACGATTAC GGGAGGTAAC ACTGGAGTCA GAGCGTAACA GAGCTCAGAT GCGTGGCCTA 
CAAGAGCAGT TTTCCAGAAT GGAGGAAACG GTGAGAAATC TACTACAGAG CCAAGGATCC 
CCAGGCCAGA ATGGAGAAGG AACTGTCAAT ATCATGAAGG CATATCAGGA AAAGCTGTCA 
GAAGACAGTA GGAAATGCAA AGAAGTCATG GAAAAAAATC ACACAGCAGT AGATGAAGAT 
TCAAGAAATG AAAGCAACAG CACTGAAGAG GAAAAAGAAA AGACAAAATT GCTACTGGAG 
CGTTTGAAAG CTCTGGAGGC AGAGAATTCA GCACTGGCTC TGGAAAATGA AAATCAGAGG 
GAACAGTATG AAAGATGTCT TGATGAGGTA GCTAATCAGG TCGTCCAGGC TCTACTTACT 
CAAAAGGATC TGAGGGAGGA ATGTATAAAG CTGAAAACGA GAGTTTTTGA TTTGGAGCAA 
CAGAATCAAA CATTAAGTGT GCTTTTCCAG CAGAGAGTCA AACCAGCTTC TGACCTCCTC 
CTTCAGAAAC TCCATTCGCG CATCTTAGAC CTCTCATCTG GAGATTTGCT GTCAGAAGTA 
GATCGAAACA GAAGCTCGGT GCCGTCACGA CCTGCTGATG CTCAGATTCA CGAATGCCAG 
CAGAATGTGA AATCCACTAT ACCTGTCTTG AAATGCCACA GTCAATTAAA TGTGACAGGT 
CCTAGCCGCC TGTATCCCCG GAGCAGCTGC AGTAGCAGTG AACTCTCCCT TTCAAGTGCT 
TGCAGTGAAT ATTCCAGTGG TTCCTCCTAC ACTTGGAATG ATGGGAAGAC GTGCAGCAAA 
AGGTCATCCG TGAGCTGGGA TAAAAGAATA AGCATTGGTT CTTCACTCCC AAGCAACCTC 
TCTAGTCCTG CAGATGATCT GCCTCCAACT CGTATCAAGG AAAACCATAT CCTAGAAGGA 
CTGAAGAAAT TACAGAGGCA AAAAGTATTA CTTGAATCAC CTTCAGTAAT ACCGAAATGG 
GGTTACAAAG ATTGCATGAA CTCCAATGAA GGAATATATT CCCCTGGAGT TAAATGTGGC 
AGCCATCATG AGCAGACTCA TTGTAAGCCA ATGGAAATAG GAAGTAGTTG CATTGAACAT 
AACAAAACTT TCTCTTATGA TTCAGACTCA CATGATGATG CAGATGATGA TTCTTCATCT 
TTATTATTGC TGCAGGAAGT ACCAAGCAAA GACTGCAGGA TCTATTGTAA CAAATTAACT 
CACAGTGTTT CTGACAGTCT CTTTCACTGG GATCCTAATA GGAAACATTT GCCAGAAAGA 
AGTTCATATT TTAATTCAAA GGAAAGACCA GAAAAATTGA CCAGTTTTGT CAGTGGATTT 
CAGGCAGAAG TAGAATCGTG CACCAGAACA AAGCTGCCTG ATTTACAGTT AGATCAAAGC 
CATGCTAATA TAGGCTGGAA GGACCTTAAT TTTCAGCTTT CAGATACTGA TGACAATGAA 
GTCTTGGATG AATTGCATAT AGAAAGCAGT GATGAGAAAA GTCCATCAGA TTTATTAGCA 
ACTCCTTTTA CTGAGAAGTA CACAAAGAAC TCTGATGTGC TCATAGTCAC AGAGAATTCT 
CAGTTTATAT CTAGTGACAA AGAGGAAAGA CAGACATCTG CTCACTCAGA CATTAGGCCA 
AAGACATGTA ATTTCATTAA ACAACAAAAA GTCATAAAAA AGACTTCTTC TGAGGAGTGC 
ATTACAGTGA TATTTGATGC TGAGGACGGT GAGCCTATTG AATTCAGCTC GCATCAAATT 
GGAGTTGTCA CTGTAACAAG AAATGAGATA TCAGTCAACC AACCAGAAAG TAGGTCCAGT 
GCAGAATATA CTGAACTTAT ACCTCAGGGA ATAGCTAATT TGCAGGCAGG AACAAATGCT 
AGAGACTACA GTGTTTTGGA AGGACCTGAA GACAAAACTA AAAAACAGAC TCTTGAAGAT 
AATACAGGGA ATAAAAATAT GGTTGTCACC ACAACCTGCA GTGAATCTTC ACCCTGTGGA 
AGAATCATAC TGCAAAATGC ACCACGACAA AAACTAGTGA AGCCAGTGTA TGATGTGTCG 
TACAAGACCA ATTCAAGCTC CATTGTTCAT GCAGGAGTCA ATCAAAAGCC AATCTTGACT 
AAAATACCCA GCAGAGGTAA ATTTTCTCCA CAAAAAAACA AGATGACAGA GAGTGAGGAG 
GCACCTGTAG CTACTTCAGT GACCCCTGCA ACCCTTGAAA AATCACCTGC TCTTCCACCT 
GTAAAGCTTT CAAGACTCAA AAAGGCACAA AGTCCACCTC GTAATTTTGA CTCAAAAGTT 
GACTTCCAAG TTCCAAAGCC CCCTGCCCAC CCCCTGCCCA GCTCAAAGCG TCCGAGCAGA 
GGCAATGGGA CAAAGTATCC AAAGAGTCAG AGTCCAGCAT CACCACTGTT GCCTCAGCAC 
CTTATAGAGC CTACTGACTA TGGAGAACCT CCAACTAGAG ACTCTAATCA TGATTTAGCA 
ACTGTAGAAG CCAGGTCGCC ATCCCCACCC CTTCCTCCCG GCAGGTCAAC GTCCCTATTG 
ATTCGTCCTG ATTATGCTCA TTCACAACCT GCGGCTGTGA AGTTAGGAGG AGCTGTTCCA 
GGTGAAACAG CAAAAAATAT ACTGAAACCA TCACCATTAA AAGGAACTGC AAACTCTGGC 
ATCCATAATC AATCTAGTCA TGATATGCAA GCAAAAAGTC TATCTTCTGG GGCCAAAATT 
GAGTTATCTT ACCTTCAAGA AAATTCTGAA GCAATTATGC AAAATAAATG TATATCTCAG 
CAACCCCATA GTGCATGCCA AGGACTTTCC AAAGTTTCCA CAAAGCAAGT CTGCCTGAAA 
AACACCAATC AGATGGGTCT CCACAGGAAC CGTGAGTTTC TGAGCATGGA TTCTCAGACA 
GGCAGGTCCA TTCCTCTGGG TCCGTCACTG GAAACAACTT CACAAGAAGT GCATTCCAGC 
AAGAAGGATA TTTCCAGTGA CAGTGGGGTT GATCAGCCAC AAAAGATGCC AAACATTCTC 
CCTGCAACTC CTCAATGTCA TACAGCAAGC GTGAGTGAGC CCTTGCTATC TCAGGTAAAC 
AATTCTCCAT TGTCAACACT GTTTCATGGT AGTGACACTG CAAATGCCAC ACAAAACTCT 
AATGAGAAAG GAGTGAAAAC CCGTCTCCCT GTAGGACTGA AATTATTTGT CAAATCTCCT 
CAACTTCTCC GAAAGAGCTC CACCGTCCCA GGGAAGCAGG AAAAAGATAG TATAAATGCA 
GCTTCCAAAA GCCACGTGAG TACAAGTAAG CACAAGCAAA ATGAACGTGT GGCCACCAAA 
GGTGCAATAG ATGCTGAATT AAAGGACTCT AAGTCTGATA CCGAAATAAA AGATAATTTT 
TCTTTGAGAC TTAGTGTGGA TACAGCATCA CCTCGTTCAC ATGAAAATGG CAGCTTGGTG 
GTAGACAGAG TAGATGAATT AGAAAGCAAA CTAGTTAAGA GATCTTTTTC TTCTAGCAAC 
AAGTCACACT TGAAACCAGC TCTAGGCATG AATGGAGCGA AAGCTCGAAG TCAGAGTTTC 
AGCGTTCATA CAGGAGAAAA GTCATCTACC CTTGTGACAG AAGGTCTTGG AAAAGTACGG 
ACTCAGATTA TTACAAACAC TTCTGACAGA GGAAATTCTC TAACAAGGCA GAACTCAGCC 
ATGGAAGGAC TTCAAATCAA GGCCATTCCT GGGTCAGGAG CAACACAAGA GACTCTTTCA 
AATACTTCAA AAAACACAGA AAATGCTTAC TCTAGACAAG GTTCTCTTGG AAGTAATAGC 
AATAACCAGC ATGGAAGCCC AAGCAAACTG CCATTCAGAT CATCTCCAAA AGTAGACTTA 
TTTCGAAACA CTTCAAAATA TGATGGAAAC AAATCATTTT CTATGAAAGA TGCACGGAGT 
CTATCTGTCC AGAGTGAGAA AAGCAGTGAA AATTTAAAAC ACGAAGCTCT AAATAAAAAG 
AGTGTTTTGC CAGCAGAATT GGAGTTAGAT ACATCAGCTA CTGTATCTTC CAAACAGATT 
TCATCAATTC ATAGCTTGGG TGGAATAAAA TGTCCGCATA AACTGGAAGC AATAAAATCA 
CAGAGACAGC AGATGTCTTT AAAGTTAGCA GAAGCCCCTC AGGTTACTGA TGTTTTTAGT 
TCACCTGCTC TGCAACCTAC AATAGAGGAG AAGGTCATGT TGTGCATCCA AGAAAATGTC 
CAGAAGGGTC AGGAACAAAA CAAATCTCCC ACTGTAGAGA CTAAACAAAA GACTGGGCCC 
TCTATAGCTA GTTGGTTTGG CTTTCGAAAG AGTAAGCTGC CAGCACTGAG CAGCAGGAAA 
CCTGACGTTC CTAAAACAAA GGTAGAAAAA AAAGAACCAA GAGTTTCAGG ATTTGGAATT 
AAGCAGTCGA AATTAGAGAG AAGAAAAGAA AAAAAGCAAA CTGAACAACA CTGTGAAGTA 
GAAAATGAAA TTAACAGGAA AACGGACAGC AGTGACTTTT TAGATGATGC TATGGAGTTT 
AAAATTTTGA AGCCATCACG AAACACGTCT GGCAAGATTG GATGTGAACA ACTCAGAGGT 
TCCTCCATAG CTACATATTC ATCAAAAGAC AGTTTTATGA AGGAACTTCT AAACAGAGTT 
GATAAGAAAG CAGCTCAGCA GACAGAAAGT GGATCAAATA ATGTTTCCTA CAGGAGTGTG 
TCGAAGGGCA GCTCCCAGGG CTCCTCTCTT CACAGCAACT CTATCAGCTC TCAAGGAAAC 
CACAAGAAAA ACAGCAACAC AAAAGCTGAT ATGGAAATAC AGAATCAGAC CCTGAGTCTT 
GCTGCCAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_025152641.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025008409.1 Gallus gallus NCK associated protein 5 (NCKAP5), transcript variant X3, mRNA.

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CloneID	OGa152163
Clone ID Related Accession (Same CDS sequence)	XM_025152641.1
Accession Version	XM_025152641.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	nck-associated protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAA AGAGACTACT ACAAAAAAGG GAAATTGGGA AAAGACTTTC TCTGGACAGC 
AGCCTTGTGG ACTATATGGA CTCAAACAAA TACATAGAGC ATCTAGTGAC ACAGCTAGAG 
GAACAGCGCT GGAATTTATG GAGGGAGAAG CTGTCTGTGG CTCGGCTGCA GCGAGAAGTT 
GCGCAGAGTA AGAGCGAAGG AACCATGCGC GAGAAACTGA TACATGAGTT GGAGGAAGAA 
AGACATTTAA GACTTGAGAG TGAAAAACGA TTACGGGAGG TAACACTGGA GTCAGAGCGT 
AACAGAGCTC AGATGCGTGG CCTACAAGAG CAGTTTTCCA GAATGGAGGA AACGGTGAGA 
AATCTACTAC AGAGCCAAGG ATCCCCAGGC CAGAATGGAG AAGGAACTGT CAATATCATG 
AAGGCATATC AGGAAAAGCT GTCAGAAGAC AGTAGGAAAT GCAAAGAAGT CATGGAAAAA 
AATCACACAG CAGTAGATGA AGATTCAAGA AATGAAAGCA ACAGCACTGA AGAGGAAAAA 
GAAAAGACAA AATTGCTACT GGAGCGTTTG AAAGCTCTGG AGGCAGAGAA TTCAGCACTG 
GCTCTGGAAA ATGAAAATCA GAGGGAACAG TATGAAAGAT GTCTTGATGA GGTAGCTAAT 
CAGGTCGTCC AGGCTCTACT TACTCAAAAG GATCTGAGGG AGGAATGTAT AAAGCTGAAA 
ACGAGAGTTT TTGATTTGGA GCAACAGAAT CAAACATTAA GTGTGCTTTT CCAGCAGAGA 
GTCAAACCAG CTTCTGACCT CCTCCTTCAG AAACTCCATT CGCGCATCTT AGACCTCTCA 
TCTGGAGATT TGCTGTCAGA AGTAGATCGA AACAGAAGCT CGGTGCCGTC ACGACCTGCT 
GATGCTCAGA TTCACGAATG CCAGCAGAAT GTGAAATCCA CTATACCTGT CTTGAAATGC 
CACAGTCAAT TAAATGTGAC AGGTCCTAGC CGCCTGTATC CCCGGAGCAG CTGCAGTAGC 
AGTGAACTCT CCCTTTCAAG TGCTTGCAGT GAATATTCCA GTGGTTCCTC CTACACTTGG 
AATGATGGGA AGACGTGCAG CAAAAGGTCA TCCGTGAGCT GGGATAAAAG AATAAGCATT 
GGTTCTTCAC TCCCAAGCAA CCTCTCTAGT CCTGCAGATG ATCTGCCTCC AACTCGTATC 
AAGGAAAACC ATATCCTAGA AGGACTGAAG AAATTACAGA GGCAAAAAGT ATTACTTGAA 
TCACCTTCAG TAATACCGAA ATGGGGTTAC AAAGATTGCA TGAACTCCAA TGAAGGAATA 
TATTCCCCTG GAGTTAAATG TGGCAGCCAT CATGAGCAGA CTCATTGTAA GCCAATGGAA 
ATAGGAAGTA GTTGCATTGA ACATAACAAA ACTTTCTCTT ATGATTCAGA CTCACATGAT 
GATGCAGATG ATGATTCTTC ATCTTTATTA TTGCTGCAGG AAGTACCAAG CAAAGACTGC 
AGGATCTATT GTAACAAATT AACTCACAGT GTTTCTGACA GTCTCTTTCA CTGGGATCCT 
AATAGGAAAC ATTTGCCAGA AAGAAGTTCA TATTTTAATT CAAAGGAAAG ACCAGAAAAA 
TTGACCAGTT TTGTCAGTGG ATTTCAGGCA GAAGTAGAAT CGTGCACCAG AACAAAGCTG 
CCTGATTTAC AGTTAGATCA AAGCCATGCT AATATAGGCT GGAAGGACCT TAATTTTCAG 
CTTTCAGATA CTGATGACAA TGAAGTCTTG GATGAATTGC ATATAGAAAG CAGTGATGAG 
AAAAGTCCAT CAGATTTATT AGCAACTCCT TTTACTGAGA AGTACACAAA GAACTCTGAT 
GTGCTCATAG TCACAGAGAA TTCTCAGTTT ATATCTAGTG ACAAAGAGGA AAGACAGACA 
TCTGCTCACT CAGACATTAG GCCAAAGACA TGTAATTTCA TTAAACAACA AAAAGTCATA 
AAAAAGACTT CTTCTGAGGA GTGCATTACA GTGATATTTG ATGCTGAGGA CGGTGAGCCT 
ATTGAATTCA GCTCGCATCA AATTGGAGTT GTCACTGTAA CAAGAAATGA GATATCAGTC 
AACCAACCAG AAAGTAGGTC CAGTGCAGAA TATACTGAAC TTATACCTCA GGGAATAGCT 
AATTTGCAGG CAGGAACAAA TGCTAGAGAC TACAGTGTTT TGGAAGGACC TGAAGACAAA 
ACTAAAAAAC AGACTCTTGA AGATAATACA GGGAATAAAA ATATGGTTGT CACCACAACC 
TGCAGTGAAT CTTCACCCTG TGGAAGAATC ATACTGCAAA ATGCACCACG ACAAAAACTA 
GTGAAGCCAG TGTATGATGT GTCGTACAAG ACCAATTCAA GCTCCATTGT TCATGCAGGA 
GTCAATCAAA AGCCAATCTT GACTAAAATA CCCAGCAGAG GTAAATTTTC TCCACAAAAA 
AACAAGATGA CAGAGAGTGA GGAGGCACCT GTAGCTACTT CAGTGACCCC TGCAACCCTT 
GAAAAATCAC CTGCTCTTCC ACCTGTAAAG CTTTCAAGAC TCAAAAAGGC ACAAAGTCCA 
CCTCGTAATT TTGACTCAAA AGTTGACTTC CAAGTTCCAA AGCCCCCTGC CCACCCCCTG 
CCCAGCTCAA AGCGTCCGAG CAGAGGCAAT GGGACAAAGT ATCCAAAGAG TCAGAGTCCA 
GCATCACCAC TGTTGCCTCA GCACCTTATA GAGCCTACTG ACTATGGAGA ACCTCCAACT 
AGAGACTCTA ATCATGATTT AGCAACTGTA GAAGCCAGGT CGCCATCCCC ACCCCTTCCT 
CCCGGCAGGT CAACGTCCCT ATTGATTCGT CCTGATTATG CTCATTCACA ACCTGCGGCT 
GTGAAGTTAG GAGGAGCTGT TCCAGGTGAA ACAGCAAAAA ATATACTGAA ACCATCACCA 
TTAAAAGGAA CTGCAAACTC TGGCATCCAT AATCAATCTA GTCATGATAT GCAAGCAAAA 
AGTCTATCTT CTGGGGCCAA AATTGAGTTA TCTTACCTTC AAGAAAATTC TGAAGCAATT 
ATGCAAAATA AATGTATATC TCAGCAACCC CATAGTGCAT GCCAAGGACT TTCCAAAGTT 
TCCACAAAGC AAGTCTGCCT GAAAAACACC AATCAGATGG GTCTCCACAG GAACCGTGAG 
TTTCTGAGCA TGGATTCTCA GACAGGCAGG TCCATTCCTC TGGGTCCGTC ACTGGAAACA 
ACTTCACAAG AAGTGCATTC CAGCAAGAAG GATATTTCCA GTGACAGTGG GGTTGATCAG 
CCACAAAAGA TGCCAAACAT TCTCCCTGCA ACTCCTCAAT GTCATACAGC AAGCGTGAGT 
GAGCCCTTGC TATCTCAGGT AAACAATTCT CCATTGTCAA CACTGTTTCA TGGTAGTGAC 
ACTGCAAATG CCACACAAAA CTCTAATGAG AAAGGAGTGA AAACCCGTCT CCCTGTAGGA 
CTGAAATTAT TTGTCAAATC TCCTCAACTT CTCCGAAAGA GCTCCACCGT CCCAGGGAAG 
CAGGAAAAAG ATAGTATAAA TGCAGCTTCC AAAAGCCACG TGAGTACAAG TAAGCACAAG 
CAAAATGAAC GTGTGGCCAC CAAAGGTGCA ATAGATGCTG AATTAAAGGA CTCTAAGTCT 
GATACCGAAA TAAAAGATAA TTTTTCTTTG AGACTTAGTG TGGATACAGC ATCACCTCGT 
TCACATGAAA ATGGCAGCTT GGTGGTAGAC AGAGTAGATG AATTAGAAAG CAAACTAGTT 
AAGAGATCTT TTTCTTCTAG CAACAAGTCA CACTTGAAAC CAGCTCTAGG CATGAATGGA 
GCGAAAGCTC GAAGTCAGAG TTTCAGCGTT CATACAGGAG AAAAGTCATC TACCCTTGTG 
ACAGAAGGTC TTGGAAAAGT ACGGACTCAG ATTATTACAA ACACTTCTGA CAGAGGAAAT 
TCTCTAACAA GGCAGAACTC AGCCATGGAA GGACTTCAAA TCAAGGCCAT TCCTGGGTCA 
GGAGCAACAC AAGAGACTCT TTCAAATACT TCAAAAAACA CAGAAAATGC TTACTCTAGA 
CAAGGTTCTC TTGGAAGTAA TAGCAATAAC CAGCATGGAA GCCCAAGCAA ACTGCCATTC 
AGATCATCTC CAAAAGTAGA CTTATTTCGA AACACTTCAA AATATGATGG AAACAAATCA 
TTTTCTATGA AAGATGCACG GAGTCTATCT GTCCAGAGTG AGAAAAGCAG TGAAAATTTA 
AAACACGAAG CTCTAAATAA AAAGAGTGTT TTGCCAGCAG AATTGGAGTT AGATACATCA 
GCTACTGTAT CTTCCAAACA GATTTCATCA ATTCATAGCT TGGGTGGAAT AAAATGTCCG 
CATAAACTGG AAGCAATAAA ATCACAGAGA CAGCAGATGT CTTTAAAGTT AGCAGAAGCC 
CCTCAGGTTA CTGATGTTTT TAGTTCACCT GCTCTGCAAC CTACAATAGA GGAGAAGGTC 
ATGTTGTGCA TCCAAGAAAA TGTCCAGAAG GGTCAGGAAC AAAACAAATC TCCCACTGTA 
GAGACTAAAC AAAAGACTGG GCCCTCTATA GCTAGTTGGT TTGGCTTTCG AAAGAGTAAG 
CTGCCAGCAC TGAGCAGCAG GAAACCTGAC GTTCCTAAAA CAAAGGTAGA AAAAAAAGAA 
CCAAGAGTTT CAGGATTTGG AATTAAGCAG TCGAAATTAG AGAGAAGAAA AGAAAAAAAG 
CAAACTGAAC AACACTGTGA AGTAGAAAAT GAAATTAACA GGAAAACGGA CAGCAGTGAC 
TTTTTAGATG ATGCTATGGA GTTTAAAATT TTGAAGCCAT CACGAAACAC GTCTGGCAAG 
ATTGGATGTG AACAACTCAG AGGTTCCTCC ATAGCTACAT ATTCATCAAA AGACAGTTTT 
ATGAAGGAAC TTCTAAACAG AGTTGATAAG AAAGCAGCTC AGCAGACAGA AAGTGGATCA 
AATAATGTTT CCTACAGGAG TGTGTCGAAG GGCAGCTCCC AGGGCTCCTC TCTTCACAGC 
AACTCTATCA GCTCTCAAGG AAACCACAAG AAAAACAGCA ACACAAAAGC TGATATGGAA 
ATACAGAATC AGACCCTGGC TAACGTAGTC GCAGAAAACC TGCAAGAAGA AGAGGAGGAC 
ACCATGACAA GGACTACATG TCAGAGTCAT GTGATAGAGT CTTGCTGCCA GATGAGGACG 
TTGGACAGCG GGATTGGAAC CTTCCCACTC CCCGACTCAG GGAACCGATC TGCGGGGCGA 
CACGTGTCTA AGCAGGAATT AACCTTGGAA ACAGAAGCCC TAGCACCGTC TGAGCAAGTT 
CTTCTTTCAG CTCCTTCAGT AAAAGCCAAA ACTCTTGAGC GAGAAGTGCC TTCAACAGCT 
ACGAGTCAGG AGTCTGTGGA AAGCATGATT AGTCACTCAA CATCTGATCC AGCCATGACT 
GCCAAAGGAA TAATTAACCT TGCAAAGCAA AGTGAACAAG AACCAAGTTC TGTGACCTCT 
ACCTCATTAC AGCTTACTGA AGAAACTGTA GAAAATAGAA AAGTTCTTTC AGATTGGACC 
ACCAAGAAAA CTACAAAAGC AAAGGACAGA ACTCTAAGAG TGTGCACTTA CTCTGCCAGC 
AGCAGTGACG CTGAACCGGA GCCAGAGTAT GGAACAAATT ATTTTCAAAC TGCAGAGGAG 
ATGTTAGTAG AGTTAACAAA GAGCAACAAA CAAGCTGAGC AGGAAAAACA GAAGCAGAGA 
AAGTCAGTTT TGGGGAACCC GATGTCAATC TTGGATTTAT ACCAGCACAG TCTCTATGGC 
CATTTTGGAG AGGATGGACC TGAACAATTA ACACATTTCA GTTTAATTGA GCAGCTGAGT 
GAACCTTCCG GTAAAGACAG CAAGGACAAA GAGAGCCCAA GTAACCTGAA GCAAACTGAA 
GAAACAAAAG AAGATCCTCA GACTAGATTA TCTAAAATTT CCCTGGAGTC TCTCAATAAA 
TTTAACAGCA ATAACGTCCT TTTATTAGAA AAGGAGAAGA ACTGTCTGAA CAAAGCTGAA 
GGACAAAAGG AAGAAAGCGG AAAGAAGGAA GCATCTTCCT TAAACAGCTC CGATAGGCAG 
GACATGGACA ATTTGGAGTC CCTGAGCGAT TCTTTGTACG ACAGCTTCTC CTCTTGTGCC 
AGCCAAGGCT CTAACGACGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025008409.1
CDS	287..6490
Translation	MERKRLLQKREIGKRLSLDSSLVDYMDSNKYIEHLVTQLEEQRWNLWREKLSVARLQREVAQSKSEGTMREKLIHELEEERHLRLESEKRLREVTLESERNRAQMRGLQEQFSRMEETVRNLLQSQGSPGQNGEGTVNIMKAYQEKLSEDSRKCKEVMEKNHTAVDEDSRNESNSTEEEKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECIKLKTRVFDLEQQNQTLSVLFQQRVKPASDLLLQKLHSRILDLSSGDLLSEVDRNRSSVPSRPADAQIHECQQNVKSTIPVLKCHSQLNVTGPSRLYPRSSCSSSELSLSSACSEYSSGSSYTWNDGKTCSKRSSVSWDKRISIGSSLPSNLSSPADDLPPTRIKENHILEGLKKLQRQKVLLESPSVIPKWGYKDCMNSNEGIYSPGVKCGSHHEQTHCKPMEIGSSCIEHNKTFSYDSDSHDDADDDSSSLLLLQEVPSKDCRIYCNKLTHSVSDSLFHWDPNRKHLPERSSYFNSKERPEKLTSFVSGFQAEVESCTRTKLPDLQLDQSHANIGWKDLNFQLSDTDDNEVLDELHIESSDEKSPSDLLATPFTEKYTKNSDVLIVTENSQFISSDKEERQTSAHSDIRPKTCNFIKQQKVIKKTSSEECITVIFDAEDGEPIEFSSHQIGVVTVTRNEISVNQPESRSSAEYTELIPQGIANLQAGTNARDYSVLEGPEDKTKKQTLEDNTGNKNMVVTTTCSESSPCGRIILQNAPRQKLVKPVYDVSYKTNSSSIVHAGVNQKPILTKIPSRGKFSPQKNKMTESEEAPVATSVTPATLEKSPALPPVKLSRLKKAQSPPRNFDSKVDFQVPKPPAHPLPSSKRPSRGNGTKYPKSQSPASPLLPQHLIEPTDYGEPPTRDSNHDLATVEARSPSPPLPPGRSTSLLIRPDYAHSQPAAVKLGGAVPGETAKNILKPSPLKGTANSGIHNQSSHDMQAKSLSSGAKIELSYLQENSEAIMQNKCISQQPHSACQGLSKVSTKQVCLKNTNQMGLHRNREFLSMDSQTGRSIPLGPSLETTSQEVHSSKKDISSDSGVDQPQKMPNILPATPQCHTASVSEPLLSQVNNSPLSTLFHGSDTANATQNSNEKGVKTRLPVGLKLFVKSPQLLRKSSTVPGKQEKDSINAASKSHVSTSKHKQNERVATKGAIDAELKDSKSDTEIKDNFSLRLSVDTASPRSHENGSLVVDRVDELESKLVKRSFSSSNKSHLKPALGMNGAKARSQSFSVHTGEKSSTLVTEGLGKVRTQIITNTSDRGNSLTRQNSAMEGLQIKAIPGSGATQETLSNTSKNTENAYSRQGSLGSNSNNQHGSPSKLPFRSSPKVDLFRNTSKYDGNKSFSMKDARSLSVQSEKSSENLKHEALNKKSVLPAELELDTSATVSSKQISSIHSLGGIKCPHKLEAIKSQRQQMSLKLAEAPQVTDVFSSPALQPTIEEKVMLCIQENVQKGQEQNKSPTVETKQKTGPSIASWFGFRKSKLPALSSRKPDVPKTKVEKKEPRVSGFGIKQSKLERRKEKKQTEQHCEVENEINRKTDSSDFLDDAMEFKILKPSRNTSGKIGCEQLRGSSIATYSSKDSFMKELLNRVDKKAAQQTESGSNNVSYRSVSKGSSQGSSLHSNSISSQGNHKKNSNTKADMEIQNQTLANVVAENLQEEEEDTMTRTTCQSHVIESCCQMRTLDSGIGTFPLPDSGNRSAGRHVSKQELTLETEALAPSEQVLLSAPSVKAKTLEREVPSTATSQESVESMISHSTSDPAMTAKGIINLAKQSEQEPSSVTSTSLQLTEETVENRKVLSDWTTKKTTKAKDRTLRVCTYSASSSDAEPEPEYGTNYFQTAEEMLVELTKSNKQAEQEKQKQRKSVLGNPMSILDLYQHSLYGHFGEDGPEQLTHFSLIEQLSEPSGKDSKDKESPSNLKQTEETKEDPQTRLSKISLESLNKFNSNNVLLLEKEKNCLNKAEGQKEESGKKEASSLNSSDRQDMDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_025152641.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NCK associated protein 5 (NCKAP5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025152641.1

ORF Insert Sequence:

ATGGAGAGAA AGAGACTACT ACAAAAAAGG GAAATTGGGA AAAGACTTTC TCTGGACAGC 
AGCCTTGTGG ACTATATGGA CTCAAACAAA TACATAGAGC ATCTAGTGAC ACAGCTAGAG 
GAACAGCGCT GGAATTTATG GAGGGAGAAG CTGTCTGTGG CTCGGCTGCA GCGAGAAGTT 
GCGCAGAGTA AGAGCGAAGG AACCATGCGC GAGAAACTGA TACATGAGTT GGAGGAAGAA 
AGACATTTAA GACTTGAGAG TGAAAAACGA TTACGGGAGG TAACACTGGA GTCAGAGCGT 
AACAGAGCTC AGATGCGTGG CCTACAAGAG CAGTTTTCCA GAATGGAGGA AACGGTGAGA 
AATCTACTAC AGAGCCAAGG ATCCCCAGGC CAGAATGGAG AAGGAACTGT CAATATCATG 
AAGGCATATC AGGAAAAGCT GTCAGAAGAC AGTAGGAAAT GCAAAGAAGT CATGGAAAAA 
AATCACACAG CAGTAGATGA AGATTCAAGA AATGAAAGCA ACAGCACTGA AGAGGAAAAA 
GAAAAGACAA AATTGCTACT GGAGCGTTTG AAAGCTCTGG AGGCAGAGAA TTCAGCACTG 
GCTCTGGAAA ATGAAAATCA GAGGGAACAG TATGAAAGAT GTCTTGATGA GGTAGCTAAT 
CAGGTCGTCC AGGCTCTACT TACTCAAAAG GATCTGAGGG AGGAATGTAT AAAGCTGAAA 
ACGAGAGTTT TTGATTTGGA GCAACAGAAT CAAACATTAA GTGTGCTTTT CCAGCAGAGA 
GTCAAACCAG CTTCTGACCT CCTCCTTCAG AAACTCCATT CGCGCATCTT AGACCTCTCA 
TCTGGAGATT TGCTGTCAGA AGTAGATCGA AACAGAAGCT CGGTGCCGTC ACGACCTGCT 
GATGCTCAGA TTCACGAATG CCAGCAGAAT GTGAAATCCA CTATACCTGT CTTGAAATGC 
CACAGTCAAT TAAATGTGAC AGGTCCTAGC CGCCTGTATC CCCGGAGCAG CTGCAGTAGC 
AGTGAACTCT CCCTTTCAAG TGCTTGCAGT GAATATTCCA GTGGTTCCTC CTACACTTGG 
AATGATGGGA AGACGTGCAG CAAAAGGTCA TCCGTGAGCT GGGATAAAAG AATAAGCATT 
GGTTCTTCAC TCCCAAGCAA CCTCTCTAGT CCTGCAGATG ATCTGCCTCC AACTCGTATC 
AAGGAAAACC ATATCCTAGA AGGACTGAAG AAATTACAGA GGCAAAAAGT ATTACTTGAA 
TCACCTTCAG TAATACCGAA ATGGGGTTAC AAAGATTGCA TGAACTCCAA TGAAGGAATA 
TATTCCCCTG GAGTTAAATG TGGCAGCCAT CATGAGCAGA CTCATTGTAA GCCAATGGAA 
ATAGGAAGTA GTTGCATTGA ACATAACAAA ACTTTCTCTT ATGATTCAGA CTCACATGAT 
GATGCAGATG ATGATTCTTC ATCTTTATTA TTGCTGCAGG AAGTACCAAG CAAAGACTGC 
AGGATCTATT GTAACAAATT AACTCACAGT GTTTCTGACA GTCTCTTTCA CTGGGATCCT 
AATAGGAAAC ATTTGCCAGA AAGAAGTTCA TATTTTAATT CAAAGGAAAG ACCAGAAAAA 
TTGACCAGTT TTGTCAGTGG ATTTCAGGCA GAAGTAGAAT CGTGCACCAG AACAAAGCTG 
CCTGATTTAC AGTTAGATCA AAGCCATGCT AATATAGGCT GGAAGGACCT TAATTTTCAG 
CTTTCAGATA CTGATGACAA TGAAGTCTTG GATGAATTGC ATATAGAAAG CAGTGATGAG 
AAAAGTCCAT CAGATTTATT AGCAACTCCT TTTACTGAGA AGTACACAAA GAACTCTGAT 
GTGCTCATAG TCACAGAGAA TTCTCAGTTT ATATCTAGTG ACAAAGAGGA AAGACAGACA 
TCTGCTCACT CAGACATTAG GCCAAAGACA TGTAATTTCA TTAAACAACA AAAAGTCATA 
AAAAAGACTT CTTCTGAGGA GTGCATTACA GTGATATTTG ATGCTGAGGA CGGTGAGCCT 
ATTGAATTCA GCTCGCATCA AATTGGAGTT GTCACTGTAA CAAGAAATGA GATATCAGTC 
AACCAACCAG AAAGTAGGTC CAGTGCAGAA TATACTGAAC TTATACCTCA GGGAATAGCT 
AATTTGCAGG CAGGAACAAA TGCTAGAGAC TACAGTGTTT TGGAAGGACC TGAAGACAAA 
ACTAAAAAAC AGACTCTTGA AGATAATACA GGGAATAAAA ATATGGTTGT CACCACAACC 
TGCAGTGAAT CTTCACCCTG TGGAAGAATC ATACTGCAAA ATGCACCACG ACAAAAACTA 
GTGAAGCCAG TGTATGATGT GTCGTACAAG ACCAATTCAA GCTCCATTGT TCATGCAGGA 
GTCAATCAAA AGCCAATCTT GACTAAAATA CCCAGCAGAG GTAAATTTTC TCCACAAAAA 
AACAAGATGA CAGAGAGTGA GGAGGCACCT GTAGCTACTT CAGTGACCCC TGCAACCCTT 
GAAAAATCAC CTGCTCTTCC ACCTGTAAAG CTTTCAAGAC TCAAAAAGGC ACAAAGTCCA 
CCTCGTAATT TTGACTCAAA AGTTGACTTC CAAGTTCCAA AGCCCCCTGC CCACCCCCTG 
CCCAGCTCAA AGCGTCCGAG CAGAGGCAAT GGGACAAAGT ATCCAAAGAG TCAGAGTCCA 
GCATCACCAC TGTTGCCTCA GCACCTTATA GAGCCTACTG ACTATGGAGA ACCTCCAACT 
AGAGACTCTA ATCATGATTT AGCAACTGTA GAAGCCAGGT CGCCATCCCC ACCCCTTCCT 
CCCGGCAGGT CAACGTCCCT ATTGATTCGT CCTGATTATG CTCATTCACA ACCTGCGGCT 
GTGAAGTTAG GAGGAGCTGT TCCAGGTGAA ACAGCAAAAA ATATACTGAA ACCATCACCA 
TTAAAAGGAA CTGCAAACTC TGGCATCCAT AATCAATCTA GTCATGATAT GCAAGCAAAA 
AGTCTATCTT CTGGGGCCAA AATTGAGTTA TCTTACCTTC AAGAAAATTC TGAAGCAATT 
ATGCAAAATA AATGTATATC TCAGCAACCC CATAGTGCAT GCCAAGGACT TTCCAAAGTT 
TCCACAAAGC AAGTCTGCCT GAAAAACACC AATCAGATGG GTCTCCACAG GAACCGTGAG 
TTTCTGAGCA TGGATTCTCA GACAGGCAGG TCCATTCCTC TGGGTCCGTC ACTGGAAACA 
ACTTCACAAG AAGTGCATTC CAGCAAGAAG GATATTTCCA GTGACAGTGG GGTTGATCAG 
CCACAAAAGA TGCCAAACAT TCTCCCTGCA ACTCCTCAAT GTCATACAGC AAGCGTGAGT 
GAGCCCTTGC TATCTCAGGT AAACAATTCT CCATTGTCAA CACTGTTTCA TGGTAGTGAC 
ACTGCAAATG CCACACAAAA CTCTAATGAG AAAGGAGTGA AAACCCGTCT CCCTGTAGGA 
CTGAAATTAT TTGTCAAATC TCCTCAACTT CTCCGAAAGA GCTCCACCGT CCCAGGGAAG 
CAGGAAAAAG ATAGTATAAA TGCAGCTTCC AAAAGCCACG TGAGTACAAG TAAGCACAAG 
CAAAATGAAC GTGTGGCCAC CAAAGGTGCA ATAGATGCTG AATTAAAGGA CTCTAAGTCT 
GATACCGAAA TAAAAGATAA TTTTTCTTTG AGACTTAGTG TGGATACAGC ATCACCTCGT 
TCACATGAAA ATGGCAGCTT GGTGGTAGAC AGAGTAGATG AATTAGAAAG CAAACTAGTT 
AAGAGATCTT TTTCTTCTAG CAACAAGTCA CACTTGAAAC CAGCTCTAGG CATGAATGGA 
GCGAAAGCTC GAAGTCAGAG TTTCAGCGTT CATACAGGAG AAAAGTCATC TACCCTTGTG 
ACAGAAGGTC TTGGAAAAGT ACGGACTCAG ATTATTACAA ACACTTCTGA CAGAGGAAAT 
TCTCTAACAA GGCAGAACTC AGCCATGGAA GGACTTCAAA TCAAGGCCAT TCCTGGGTCA 
GGAGCAACAC AAGAGACTCT TTCAAATACT TCAAAAAACA CAGAAAATGC TTACTCTAGA 
CAAGGTTCTC TTGGAAGTAA TAGCAATAAC CAGCATGGAA GCCCAAGCAA ACTGCCATTC 
AGATCATCTC CAAAAGTAGA CTTATTTCGA AACACTTCAA AATATGATGG AAACAAATCA 
TTTTCTATGA AAGATGCACG GAGTCTATCT GTCCAGAGTG AGAAAAGCAG TGAAAATTTA 
AAACACGAAG CTCTAAATAA AAAGAGTGTT TTGCCAGCAG AATTGGAGTT AGATACATCA 
GCTACTGTAT CTTCCAAACA GATTTCATCA ATTCATAGCT TGGGTGGAAT AAAATGTCCG 
CATAAACTGG AAGCAATAAA ATCACAGAGA CAGCAGATGT CTTTAAAGTT AGCAGAAGCC 
CCTCAGGTTA CTGATGTTTT TAGTTCACCT GCTCTGCAAC CTACAATAGA GGAGAAGGTC 
ATGTTGTGCA TCCAAGAAAA TGTCCAGAAG GGTCAGGAAC AAAACAAATC TCCCACTGTA 
GAGACTAAAC AAAAGACTGG GCCCTCTATA GCTAGTTGGT TTGGCTTTCG AAAGAGTAAG 
CTGCCAGCAC TGAGCAGCAG GAAACCTGAC GTTCCTAAAA CAAAGGTAGA AAAAAAAGAA 
CCAAGAGTTT CAGGATTTGG AATTAAGCAG TCGAAATTAG AGAGAAGAAA AGAAAAAAAG 
CAAACTGAAC AACACTGTGA AGTAGAAAAT GAAATTAACA GGAAAACGGA CAGCAGTGAC 
TTTTTAGATG ATGCTATGGA GTTTAAAATT TTGAAGCCAT CACGAAACAC GTCTGGCAAG 
ATTGGATGTG AACAACTCAG AGGTTCCTCC ATAGCTACAT ATTCATCAAA AGACAGTTTT 
ATGAAGGAAC TTCTAAACAG AGTTGATAAG AAAGCAGCTC AGCAGACAGA AAGTGGATCA 
AATAATGTTT CCTACAGGAG TGTGTCGAAG GGCAGCTCCC AGGGCTCCTC TCTTCACAGC 
AACTCTATCA GCTCTCAAGG AAACCACAAG AAAAACAGCA ACACAAAAGC TGATATGGAA 
ATACAGAATC AGACCCTGGC TAACGTAGTC GCAGAAAACC TGCAAGAAGA AGAGGAGGAC 
ACCATGACAA GGACTACATG TCAGAGTCAT GTGATAGAGT CTTGCTGCCA GATGAGGACG 
TTGGACAGCG GGATTGGAAC CTTCCCACTC CCCGACTCAG GGAACCGATC TGCGGGGCGA 
CACGTGTCTA AGCAGGAATT AACCTTGGAA ACAGAAGCCC TAGCACCGTC TGAGCAAGTT 
CTTCTTTCAG CTCCTTCAGT AAAAGCCAAA ACTCTTGAGC GAGAAGTGCC TTCAACAGCT 
ACGAGTCAGG AGTCTGTGGA AAGCATGATT AGTCACTCAA CATCTGATCC AGCCATGACT 
GCCAAAGGAA TAATTAACCT TGCAAAGCAA AGTGAACAAG AACCAAGTTC TGTGACCTCT 
ACCTCATTAC AGCTTACTGA AGAAACTGTA GAAAATAGAA AAGTTCTTTC AGATTGGACC 
ACCAAGAAAA CTACAAAAGC AAAGGACAGA ACTCTAAGAG TGTGCACTTA CTCTGCCAGC 
AGCAGTGACG CTGAACCGGA GCCAGAGTAT GGAACAAATT ATTTTCAAAC TGCAGAGGAG 
ATGTTAGTAG AGTTAACAAA GAGCAACAAA CAAGCTGAGC AGGAAAAACA GAAGCAGAGA 
AAGTCAGTTT TGGGGAACCC GATGTCAATC TTGGATTTAT ACCAGCACAG TCTCTATGGC 
CATTTTGGAG AGGATGGACC TGAACAATTA ACACATTTCA GTTTAATTGA GCAGCTGAGT 
GAACCTTCCG GTAAAGACAG CAAGGACAAA GAGAGCCCAA GTAACCTGAA GCAAACTGAA 
GAAACAAAAG AAGATCCTCA GACTAGATTA TCTAAAATTT CCCTGGAGTC TCTCAATAAA 
TTTAACAGCA ATAACGTCCT TTTATTAGAA AAGGAGAAGA ACTGTCTGAA CAAAGCTGAA 
GGACAAAAGG AAGAAAGCGG AAAGAAGGAA GCATCTTCCT TAAACAGCTC CGATAGGCAG 
GACATGGACA ATTTGGAGTC CCTGAGCGAT TCTTTGTACG ACAGCTTCTC CTCTTGTGCC 
AGCCAAGGCT CTAACGACGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_025152642.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025008410.1 Gallus gallus NCK associated protein 5 (NCKAP5), transcript variant X4, mRNA.

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CloneID	OGa152164
Clone ID Related Accession (Same CDS sequence)	XM_025152642.1
Accession Version	XM_025152642.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	nck-associated protein 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAA AGAGACTACT ACAAAAAAGG GAAATTGGGA AAAGACTTTC TCTGGACAGC 
AGCCTTGTGG ACTATATGGA CTCAAACAAA TACATAGAGC ATCTAGTGAC ACAGCTAGAG 
GAACAGCGCT GGAATTTATG GAGGGAGAAG CTGTCTGTGG CTCGGCTGCA GCGAGAAGTT 
GCGCAGAGTA AGAGCGAAGG AACCATGCGC GAGAAACTGA TACATGAGTT GGAGGAAGAA 
AGACATTTAA GACTTGAGAG TGAAAAACGA TTACGGGAGG TAACACTGGA GTCAGAGCGT 
AACAGAGCTC AGATGCGTGG CCTACAAGAG CAGTTTTCCA GAATGGAGGA AACGGTGAGA 
AATCTACTAC AGAGCCAAGG ATCCCCAGGC CAGAATGGAG AAGGAACTGT CAATATCATG 
AAGGCATATC AGGAAAAGCT GTCAGAAGAC AGTAGGAAAT GCAAAGAAGT CATGGAAAAA 
AATCACACAG CAGTAGATGA AGATTCAAGA AATGAAAGCA ACAGCACTGA AGAGGAAAAA 
GAAAAGACAA AATTGCTACT GGAGCGTTTG AAAGCTCTGG AGGCAGAGAA TTCAGCACTG 
GCTCTGGAAA ATGAAAATCA GAGGGAACAG TATGAAAGAT GTCTTGATGA GGTAGCTAAT 
CAGGTCGTCC AGGCTCTACT TACTCAAAAG GATCTGAGGG AGGAATGTAT AAAGCTGAAA 
ACGAGAGTTT TTGATTTGGA GCAACAGAAT CAAACATTAA GTGTGCTTTT CCAGCAGAGA 
GTCAAACCAG CTTCTGACCT CCTCCTTCAG AAACTCCATT CGCGCATCTT AGACCTCTCA 
TCTGGAGATT TGCTGTCAGA AGTAGATCGA AACAGAAGCT CGGTGCCGTC ACGACCTGCT 
GATGCTCAGA TTCACGAATG CCAGCAGAAT GTGAAATCCA CTATACCTGT CTTGAAATGC 
CACAGTCAAT TAAATGTGAC AGGTCCTAGC CGCCTGTATC CCCGGAGCAG CTGCAGTAGC 
AGTGAACTCT CCCTTTCAAG TGCTTGCAGT GAATATTCCA GTGGTTCCTC CTACACTTGG 
AATGATGGGA AGACGTGCAG CAAAAGGTCA TCCGTGAGCT GGGATAAAAG AATAAGCATT 
GGTTCTTCAC TCCCAAGCAA CCTCTCTAGT CCTGCAGATG ATCTGCCTCC AACTCGTATC 
AAGGAAAACC ATATCCTAGA AGGACTGAAG AAATTACAGA GGCAAAAAGT ATTACTTGAA 
TCACCTTCAG TAATACCGAA ATGGGGTTAC AAAGATTGCA TGAACTCCAA TGAAGGAATA 
TATTCCCCTG GAGTTAAATG TGGCAGCCAT CATGAGCAGA CTCATTGTAA GCCAATGGAA 
ATAGGAAGTA GTTGCATTGA ACATAACAAA ACTTTCTCTT ATGATTCAGA CTCACATGAT 
GATGCAGATG ATGATTCTTC ATCTTTATTA TTGCTGCAGG AAGTACCAAG CAAAGACTGC 
AGGATCTATT GTAACAAATT AACTCACAGT GTTTCTGACA GTCTCTTTCA CTGGGATCCT 
AATAGGAAAC ATTTGCCAGA AAGAAGTTCA TATTTTAATT CAAAGGAAAG ACCAGAAAAA 
TTGACCAGTT TTGTCAGTGG ATTTCAGGCA GAAGTAGAAT CGTGCACCAG AACAAAGCTG 
CCTGATTTAC AGTTAGATCA AAGCCATGCT AATATAGGCT GGAAGGACCT TAATTTTCAG 
CTTTCAGATA CTGATGACAA TGAAGTCTTG GATGAATTGC ATATAGAAAG CAGTGATGAG 
AAAAGTCCAT CAGATTTATT AGCAACTCCT TTTACTGAGA AGTACACAAA GAACTCTGAT 
GTGCTCATAG TCACAGAGAA TTCTCAGTTT ATATCTAGTG ACAAAGAGGA AAGACAGACA 
TCTGCTCACT CAGACATTAG GCCAAAGACA TGTAATTTCA TTAAACAACA AAAAGTCATA 
AAAAAGACTT CTTCTGAGGA GTGCATTACA GTGATATTTG ATGCTGAGGA CGGTGAGCCT 
ATTGAATTCA GCTCGCATCA AATTGGAGTT GTCACTGTAA CAAGAAATGA GATATCAGTC 
AACCAACCAG AAAGTAGGTC CAGTGCAGAA TATACTGAAC TTATACCTCA GGGAATAGCT 
AATTTGCAGG CAGGAACAAA TGCTAGAGAC TACAGTGTTT TGGAAGGACC TGAAGACAAA 
ACTAAAAAAC AGACTCTTGA AGATAATACA GGGAATAAAA ATATGGTTGT CACCACAACC 
TGCAGTGAAT CTTCACCCTG TGGAAGAATC ATACTGCAAA ATGCACCACG ACAAAAACTA 
GTGAAGCCAG TGTATGATGT GTCGTACAAG ACCAATTCAA GCTCCATTGT TCATGCAGGA 
GTCAATCAAA AGCCAATCTT GACTAAAATA CCCAGCAGAG GTAAATTTTC TCCACAAAAA 
AACAAGATGA CAGAGAGTGA GGAGGCACCT GTAGCTACTT CAGTGACCCC TGCAACCCTT 
GAAAAATCAC CTGCTCTTCC ACCTGTAAAG CTTTCAAGAC TCAAAAAGGC ACAAAGTCCA 
CCTCGTAATT TTGACTCAAA AGTTGACTTC CAAGTTCCAA AGCCCCCTGC CCACCCCCTG 
CCCAGCTCAA AGCGTCCGAG CAGAGGCAAT GGGACAAAGT ATCCAAAGAG TCAGAGTCCA 
GCATCACCAC TGTTGCCTCA GCACCTTATA GAGCCTACTG ACTATGGAGA ACCTCCAACT 
AGAGACTCTA ATCATGATTT AGCAACTGTA GAAGCCAGGT CGCCATCCCC ACCCCTTCCT 
CCCGGCAGGT CAACGTCCCT ATTGATTCGT CCTGATTATG CTCATTCACA ACCTGCGGCT 
GTGAAGTTAG GAGGAGCTGT TCCAGGTGAA ACAGCAAAAA ATATACTGAA ACCATCACCA 
TTAAAAGGAA CTGCAAACTC TGGCATCCAT AATCAATCTA GTCATGATAT GCAAGCAAAA 
AGTCTATCTT CTGGGGCCAA AATTGAGTTA TCTTACCTTC AAGAAAATTC TGAAGCAATT 
ATGCAAAATA AATGTATATC TCAGCAACCC CATAGTGCAT GCCAAGGACT TTCCAAAGTT 
TCCACAAAGC AAGTCTGCCT GAAAAACACC AATCAGATGG GTCTCCACAG GAACCGTGAG 
TTTCTGAGCA TGGATTCTCA GACAGGCAGG TCCATTCCTC TGGGTCCGTC ACTGGAAACA 
ACTTCACAAG AAGTGCATTC CAGCAAGAAG GATATTTCCA GTGACAGTGG GGTTGATCAG 
CCACAAAAGA TGCCAAACAT TCTCCCTGCA ACTCCTCAAT GTCATACAGC AAGCGTGAGT 
GAGCCCTTGC TATCTCAGGT AAACAATTCT CCATTGTCAA CACTGTTTCA TGGTAGTGAC 
ACTGCAAATG CCACACAAAA CTCTAATGAG AAAGGAGTGA AAACCCGTCT CCCTGTAGGA 
CTGAAATTAT TTGTCAAATC TCCTCAACTT CTCCGAAAGA GCTCCACCGT CCCAGGGAAG 
CAGGAAAAAG ATAGTATAAA TGCAGCTTCC AAAAGCCACG TGAGTACAAG TAAGCACAAG 
CAAAATGAAC GTGTGGCCAC CAAAGGTGCA ATAGATGCTG AATTAAAGGA CTCTAAGTCT 
GATACCGAAA TAAAAGATAA TTTTTCTTTG AGACTTAGTG TGGATACAGC ATCACCTCGT 
TCACATGAAA ATGGCAGCTT GGTGGTAGAC AGAGTAGATG AATTAGAAAG CAAACTAGTT 
AAGAGATCTT TTTCTTCTAG CAACAAGTCA CACTTGAAAC CAGCTCTAGG CATGAATGGA 
GCGAAAGCTC GAAGTCAGAG TTTCAGCGTT CATACAGGAG AAAAGTCATC TACCCTTGTG 
ACAGAAGGTC TTGGAAAAGT ACGGACTCAG ATTATTACAA ACACTTCTGA CAGAGGAAAT 
TCTCTAACAA GGCAGAACTC AGCCATGGAA GGACTTCAAA TCAAGGCCAT TCCTGGGTCA 
GGAGCAACAC AAGAGACTCT TTCAAATACT TCAAAAAACA CAGAAAATGC TTACTCTAGA 
CAAGGTTCTC TTGGAAGTAA TAGCAATAAC CAGCATGGAA GCCCAAGCAA ACTGCCATTC 
AGATCATCTC CAAAAGTAGA CTTATTTCGA AACACTTCAA AATATGATGG AAACAAATCA 
TTTTCTATGA AAGATGCACG GAGTCTATCT GTCCAGAGTG AGAAAAGCAG TGAAAATTTA 
AAACACGAAG CTCTAAATAA AAAGAGTGTT TTGCCAGCAG AATTGGAGTT AGATACATCA 
GCTACTGTAT CTTCCAAACA GATTTCATCA ATTCATAGCT TGGGTGGAAT AAAATGTCCG 
CATAAACTGG AAGCAATAAA ATCACAGAGA CAGCAGATGT CTTTAAAGTT AGCAGAAGCC 
CCTCAGGTTA CTGATGTTTT TAGTTCACCT GCTCTGCAAC CTACAATAGA GGAGAAGGTC 
ATGTTGTGCA TCCAAGAAAA TGTCCAGAAG GGTCAGGAAC AAAACAAATC TCCCACTGTA 
GAGACTAAAC AAAAGACTGG GCCCTCTATA GCTAGTTGGT TTGGCTTTCG AAAGAGTAAG 
CTGCCAGCAC TGAGCAGCAG GAAACCTGAC GTTCCTAAAA CAAAGGTAGA AAAAAAAGAA 
CCAAGAGTTT CAGGATTTGG AATTAAGCAG TCGAAATTAG AGAGAAGAAA AGAAAAAAAG 
CAAACTGAAC AACACTGTGA AGTAGAAAAT GAAATTAACA GGAAAACGGA CAGCAGTGAC 
TTTTTAGATG ATGCTATGGA GTTTAAAATT TTGAAGCCAT CACGAAACAC GTCTGGCAAG 
ATTGGATGTG AACAACTCAG AGGTTCCTCC ATAGCTACAT ATTCATCAAA AGACAGTTTT 
ATGAAGGAAC TTCTAAACAG GAGTGTGTCG AAGGGCAGCT CCCAGGGCTC CTCTCTTCAC 
AGCAACTCTA TCAGCTCTCA AGGAAACCAC AAGAAAAACA GCAACACAAA AGCTGATATG 
GAAATACAGA ATCAGACCCT GGCTAACGTA GTCGCAGAAA ACCTGCAAGA AGAAGAGGAG 
GACACCATGA CAAGGACTAC ATGTCAGAGT CATGTGATAG AGTCTTGCTG CCAGATGAGG 
ACGTTGGACA GCGGGATTGG AACCTTCCCA CTCCCCGACT CAGGGAACCG ATCTGCGGGG 
CGACACGTGT CTAAGCAGGA ATTAACCTTG GAAACAGAAG CCCTAGCACC GTCTGAGCAA 
GTTCTTCTTT CAGCTCCTTC AGTAAAAGCC AAAACTCTTG AGCGAGAAGT GCCTTCAACA 
GCTACGAGTC AGGAGTCTGT GGAAAGCATG ATTAGTCACT CAACATCTGA TCCAGCCATG 
ACTGCCAAAG GTATACGACC TTTTCAAAGT CGCCTTCCAA AACCAGATTC CTCAGGAATA 
ATTAACCTTG CAAAGCAAAG TGAACAAGAA CCAAGTTCTG TGACCTCTAC CTCATTACAG 
CTTACTGAAG AAACTGTAGA AAATAGAAAA GTTCTTTCAG ATTGGACCAC CAAGAAAACT 
ACAAAAGCAA AGGACAGAAC TCTAAGAGTG TGCACTTACT CTGCCAGCAG CAGTGACGCT 
GAACCGGAGC CAGAGTATGG AACAAATTAT TTTCAAACTG CAGAGGAGAT GTTAGTAGAG 
TTAACAAAGA GCAACAAACA AGCTGAGCAG GAAAAACAGA AGCAGAGAAA GTCAGTTTTG 
GGGAACCCGA TGTCAATCTT GGATTTATAC CAGCACAGTC TCTATGGCCA TTTTGGAGAG 
GATGGACCTG AACAATTAAC ACATTTCAGT TTAATTGAGC AGCTGAGTGA ACCTTCCGGT 
AAAGACAGCA AGGACAAAGA GAGCCCAAGT AACCTGAAGC AAACTGAAGA AACAAAAGAA 
GATCCTCAGA CTAGATTATC TAAAATTTCC CTGGAGTCTC TCAATAAATT TAACAGCAAT 
AACGTCCTTT TATTAGAAAA GGAGAAGAAC TGTCTGAACA AAGCTGAAGG ACAAAAGGAA 
GAAAGCGGAA AGAAGGAAGC ATCTTCCTTA AACAGCTCCG ATAGGCAGGA CATGGACAAT 
TTGGAGTCCC TGAGCGATTC TTTGTACGAC AGCTTCTCCT CTTGTGCCAG CCAAGGCTCT 
AACGACGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025008410.1
CDS	285..6476
Translation	MERKRLLQKREIGKRLSLDSSLVDYMDSNKYIEHLVTQLEEQRWNLWREKLSVARLQREVAQSKSEGTMREKLIHELEEERHLRLESEKRLREVTLESERNRAQMRGLQEQFSRMEETVRNLLQSQGSPGQNGEGTVNIMKAYQEKLSEDSRKCKEVMEKNHTAVDEDSRNESNSTEEEKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECIKLKTRVFDLEQQNQTLSVLFQQRVKPASDLLLQKLHSRILDLSSGDLLSEVDRNRSSVPSRPADAQIHECQQNVKSTIPVLKCHSQLNVTGPSRLYPRSSCSSSELSLSSACSEYSSGSSYTWNDGKTCSKRSSVSWDKRISIGSSLPSNLSSPADDLPPTRIKENHILEGLKKLQRQKVLLESPSVIPKWGYKDCMNSNEGIYSPGVKCGSHHEQTHCKPMEIGSSCIEHNKTFSYDSDSHDDADDDSSSLLLLQEVPSKDCRIYCNKLTHSVSDSLFHWDPNRKHLPERSSYFNSKERPEKLTSFVSGFQAEVESCTRTKLPDLQLDQSHANIGWKDLNFQLSDTDDNEVLDELHIESSDEKSPSDLLATPFTEKYTKNSDVLIVTENSQFISSDKEERQTSAHSDIRPKTCNFIKQQKVIKKTSSEECITVIFDAEDGEPIEFSSHQIGVVTVTRNEISVNQPESRSSAEYTELIPQGIANLQAGTNARDYSVLEGPEDKTKKQTLEDNTGNKNMVVTTTCSESSPCGRIILQNAPRQKLVKPVYDVSYKTNSSSIVHAGVNQKPILTKIPSRGKFSPQKNKMTESEEAPVATSVTPATLEKSPALPPVKLSRLKKAQSPPRNFDSKVDFQVPKPPAHPLPSSKRPSRGNGTKYPKSQSPASPLLPQHLIEPTDYGEPPTRDSNHDLATVEARSPSPPLPPGRSTSLLIRPDYAHSQPAAVKLGGAVPGETAKNILKPSPLKGTANSGIHNQSSHDMQAKSLSSGAKIELSYLQENSEAIMQNKCISQQPHSACQGLSKVSTKQVCLKNTNQMGLHRNREFLSMDSQTGRSIPLGPSLETTSQEVHSSKKDISSDSGVDQPQKMPNILPATPQCHTASVSEPLLSQVNNSPLSTLFHGSDTANATQNSNEKGVKTRLPVGLKLFVKSPQLLRKSSTVPGKQEKDSINAASKSHVSTSKHKQNERVATKGAIDAELKDSKSDTEIKDNFSLRLSVDTASPRSHENGSLVVDRVDELESKLVKRSFSSSNKSHLKPALGMNGAKARSQSFSVHTGEKSSTLVTEGLGKVRTQIITNTSDRGNSLTRQNSAMEGLQIKAIPGSGATQETLSNTSKNTENAYSRQGSLGSNSNNQHGSPSKLPFRSSPKVDLFRNTSKYDGNKSFSMKDARSLSVQSEKSSENLKHEALNKKSVLPAELELDTSATVSSKQISSIHSLGGIKCPHKLEAIKSQRQQMSLKLAEAPQVTDVFSSPALQPTIEEKVMLCIQENVQKGQEQNKSPTVETKQKTGPSIASWFGFRKSKLPALSSRKPDVPKTKVEKKEPRVSGFGIKQSKLERRKEKKQTEQHCEVENEINRKTDSSDFLDDAMEFKILKPSRNTSGKIGCEQLRGSSIATYSSKDSFMKELLNRSVSKGSSQGSSLHSNSISSQGNHKKNSNTKADMEIQNQTLANVVAENLQEEEEDTMTRTTCQSHVIESCCQMRTLDSGIGTFPLPDSGNRSAGRHVSKQELTLETEALAPSEQVLLSAPSVKAKTLEREVPSTATSQESVESMISHSTSDPAMTAKGIRPFQSRLPKPDSSGIINLAKQSEQEPSSVTSTSLQLTEETVENRKVLSDWTTKKTTKAKDRTLRVCTYSASSSDAEPEPEYGTNYFQTAEEMLVELTKSNKQAEQEKQKQRKSVLGNPMSILDLYQHSLYGHFGEDGPEQLTHFSLIEQLSEPSGKDSKDKESPSNLKQTEETKEDPQTRLSKISLESLNKFNSNNVLLLEKEKNCLNKAEGQKEESGKKEASSLNSSDRQDMDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_025152642.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NCK associated protein 5 (NCKAP5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025152642.1

ORF Insert Sequence:

ATGGAGAGAA AGAGACTACT ACAAAAAAGG GAAATTGGGA AAAGACTTTC TCTGGACAGC 
AGCCTTGTGG ACTATATGGA CTCAAACAAA TACATAGAGC ATCTAGTGAC ACAGCTAGAG 
GAACAGCGCT GGAATTTATG GAGGGAGAAG CTGTCTGTGG CTCGGCTGCA GCGAGAAGTT 
GCGCAGAGTA AGAGCGAAGG AACCATGCGC GAGAAACTGA TACATGAGTT GGAGGAAGAA 
AGACATTTAA GACTTGAGAG TGAAAAACGA TTACGGGAGG TAACACTGGA GTCAGAGCGT 
AACAGAGCTC AGATGCGTGG CCTACAAGAG CAGTTTTCCA GAATGGAGGA AACGGTGAGA 
AATCTACTAC AGAGCCAAGG ATCCCCAGGC CAGAATGGAG AAGGAACTGT CAATATCATG 
AAGGCATATC AGGAAAAGCT GTCAGAAGAC AGTAGGAAAT GCAAAGAAGT CATGGAAAAA 
AATCACACAG CAGTAGATGA AGATTCAAGA AATGAAAGCA ACAGCACTGA AGAGGAAAAA 
GAAAAGACAA AATTGCTACT GGAGCGTTTG AAAGCTCTGG AGGCAGAGAA TTCAGCACTG 
GCTCTGGAAA ATGAAAATCA GAGGGAACAG TATGAAAGAT GTCTTGATGA GGTAGCTAAT 
CAGGTCGTCC AGGCTCTACT TACTCAAAAG GATCTGAGGG AGGAATGTAT AAAGCTGAAA 
ACGAGAGTTT TTGATTTGGA GCAACAGAAT CAAACATTAA GTGTGCTTTT CCAGCAGAGA 
GTCAAACCAG CTTCTGACCT CCTCCTTCAG AAACTCCATT CGCGCATCTT AGACCTCTCA 
TCTGGAGATT TGCTGTCAGA AGTAGATCGA AACAGAAGCT CGGTGCCGTC ACGACCTGCT 
GATGCTCAGA TTCACGAATG CCAGCAGAAT GTGAAATCCA CTATACCTGT CTTGAAATGC 
CACAGTCAAT TAAATGTGAC AGGTCCTAGC CGCCTGTATC CCCGGAGCAG CTGCAGTAGC 
AGTGAACTCT CCCTTTCAAG TGCTTGCAGT GAATATTCCA GTGGTTCCTC CTACACTTGG 
AATGATGGGA AGACGTGCAG CAAAAGGTCA TCCGTGAGCT GGGATAAAAG AATAAGCATT 
GGTTCTTCAC TCCCAAGCAA CCTCTCTAGT CCTGCAGATG ATCTGCCTCC AACTCGTATC 
AAGGAAAACC ATATCCTAGA AGGACTGAAG AAATTACAGA GGCAAAAAGT ATTACTTGAA 
TCACCTTCAG TAATACCGAA ATGGGGTTAC AAAGATTGCA TGAACTCCAA TGAAGGAATA 
TATTCCCCTG GAGTTAAATG TGGCAGCCAT CATGAGCAGA CTCATTGTAA GCCAATGGAA 
ATAGGAAGTA GTTGCATTGA ACATAACAAA ACTTTCTCTT ATGATTCAGA CTCACATGAT 
GATGCAGATG ATGATTCTTC ATCTTTATTA TTGCTGCAGG AAGTACCAAG CAAAGACTGC 
AGGATCTATT GTAACAAATT AACTCACAGT GTTTCTGACA GTCTCTTTCA CTGGGATCCT 
AATAGGAAAC ATTTGCCAGA AAGAAGTTCA TATTTTAATT CAAAGGAAAG ACCAGAAAAA 
TTGACCAGTT TTGTCAGTGG ATTTCAGGCA GAAGTAGAAT CGTGCACCAG AACAAAGCTG 
CCTGATTTAC AGTTAGATCA AAGCCATGCT AATATAGGCT GGAAGGACCT TAATTTTCAG 
CTTTCAGATA CTGATGACAA TGAAGTCTTG GATGAATTGC ATATAGAAAG CAGTGATGAG 
AAAAGTCCAT CAGATTTATT AGCAACTCCT TTTACTGAGA AGTACACAAA GAACTCTGAT 
GTGCTCATAG TCACAGAGAA TTCTCAGTTT ATATCTAGTG ACAAAGAGGA AAGACAGACA 
TCTGCTCACT CAGACATTAG GCCAAAGACA TGTAATTTCA TTAAACAACA AAAAGTCATA 
AAAAAGACTT CTTCTGAGGA GTGCATTACA GTGATATTTG ATGCTGAGGA CGGTGAGCCT 
ATTGAATTCA GCTCGCATCA AATTGGAGTT GTCACTGTAA CAAGAAATGA GATATCAGTC 
AACCAACCAG AAAGTAGGTC CAGTGCAGAA TATACTGAAC TTATACCTCA GGGAATAGCT 
AATTTGCAGG CAGGAACAAA TGCTAGAGAC TACAGTGTTT TGGAAGGACC TGAAGACAAA 
ACTAAAAAAC AGACTCTTGA AGATAATACA GGGAATAAAA ATATGGTTGT CACCACAACC 
TGCAGTGAAT CTTCACCCTG TGGAAGAATC ATACTGCAAA ATGCACCACG ACAAAAACTA 
GTGAAGCCAG TGTATGATGT GTCGTACAAG ACCAATTCAA GCTCCATTGT TCATGCAGGA 
GTCAATCAAA AGCCAATCTT GACTAAAATA CCCAGCAGAG GTAAATTTTC TCCACAAAAA 
AACAAGATGA CAGAGAGTGA GGAGGCACCT GTAGCTACTT CAGTGACCCC TGCAACCCTT 
GAAAAATCAC CTGCTCTTCC ACCTGTAAAG CTTTCAAGAC TCAAAAAGGC ACAAAGTCCA 
CCTCGTAATT TTGACTCAAA AGTTGACTTC CAAGTTCCAA AGCCCCCTGC CCACCCCCTG 
CCCAGCTCAA AGCGTCCGAG CAGAGGCAAT GGGACAAAGT ATCCAAAGAG TCAGAGTCCA 
GCATCACCAC TGTTGCCTCA GCACCTTATA GAGCCTACTG ACTATGGAGA ACCTCCAACT 
AGAGACTCTA ATCATGATTT AGCAACTGTA GAAGCCAGGT CGCCATCCCC ACCCCTTCCT 
CCCGGCAGGT CAACGTCCCT ATTGATTCGT CCTGATTATG CTCATTCACA ACCTGCGGCT 
GTGAAGTTAG GAGGAGCTGT TCCAGGTGAA ACAGCAAAAA ATATACTGAA ACCATCACCA 
TTAAAAGGAA CTGCAAACTC TGGCATCCAT AATCAATCTA GTCATGATAT GCAAGCAAAA 
AGTCTATCTT CTGGGGCCAA AATTGAGTTA TCTTACCTTC AAGAAAATTC TGAAGCAATT 
ATGCAAAATA AATGTATATC TCAGCAACCC CATAGTGCAT GCCAAGGACT TTCCAAAGTT 
TCCACAAAGC AAGTCTGCCT GAAAAACACC AATCAGATGG GTCTCCACAG GAACCGTGAG 
TTTCTGAGCA TGGATTCTCA GACAGGCAGG TCCATTCCTC TGGGTCCGTC ACTGGAAACA 
ACTTCACAAG AAGTGCATTC CAGCAAGAAG GATATTTCCA GTGACAGTGG GGTTGATCAG 
CCACAAAAGA TGCCAAACAT TCTCCCTGCA ACTCCTCAAT GTCATACAGC AAGCGTGAGT 
GAGCCCTTGC TATCTCAGGT AAACAATTCT CCATTGTCAA CACTGTTTCA TGGTAGTGAC 
ACTGCAAATG CCACACAAAA CTCTAATGAG AAAGGAGTGA AAACCCGTCT CCCTGTAGGA 
CTGAAATTAT TTGTCAAATC TCCTCAACTT CTCCGAAAGA GCTCCACCGT CCCAGGGAAG 
CAGGAAAAAG ATAGTATAAA TGCAGCTTCC AAAAGCCACG TGAGTACAAG TAAGCACAAG 
CAAAATGAAC GTGTGGCCAC CAAAGGTGCA ATAGATGCTG AATTAAAGGA CTCTAAGTCT 
GATACCGAAA TAAAAGATAA TTTTTCTTTG AGACTTAGTG TGGATACAGC ATCACCTCGT 
TCACATGAAA ATGGCAGCTT GGTGGTAGAC AGAGTAGATG AATTAGAAAG CAAACTAGTT 
AAGAGATCTT TTTCTTCTAG CAACAAGTCA CACTTGAAAC CAGCTCTAGG CATGAATGGA 
GCGAAAGCTC GAAGTCAGAG TTTCAGCGTT CATACAGGAG AAAAGTCATC TACCCTTGTG 
ACAGAAGGTC TTGGAAAAGT ACGGACTCAG ATTATTACAA ACACTTCTGA CAGAGGAAAT 
TCTCTAACAA GGCAGAACTC AGCCATGGAA GGACTTCAAA TCAAGGCCAT TCCTGGGTCA 
GGAGCAACAC AAGAGACTCT TTCAAATACT TCAAAAAACA CAGAAAATGC TTACTCTAGA 
CAAGGTTCTC TTGGAAGTAA TAGCAATAAC CAGCATGGAA GCCCAAGCAA ACTGCCATTC 
AGATCATCTC CAAAAGTAGA CTTATTTCGA AACACTTCAA AATATGATGG AAACAAATCA 
TTTTCTATGA AAGATGCACG GAGTCTATCT GTCCAGAGTG AGAAAAGCAG TGAAAATTTA 
AAACACGAAG CTCTAAATAA AAAGAGTGTT TTGCCAGCAG AATTGGAGTT AGATACATCA 
GCTACTGTAT CTTCCAAACA GATTTCATCA ATTCATAGCT TGGGTGGAAT AAAATGTCCG 
CATAAACTGG AAGCAATAAA ATCACAGAGA CAGCAGATGT CTTTAAAGTT AGCAGAAGCC 
CCTCAGGTTA CTGATGTTTT TAGTTCACCT GCTCTGCAAC CTACAATAGA GGAGAAGGTC 
ATGTTGTGCA TCCAAGAAAA TGTCCAGAAG GGTCAGGAAC AAAACAAATC TCCCACTGTA 
GAGACTAAAC AAAAGACTGG GCCCTCTATA GCTAGTTGGT TTGGCTTTCG AAAGAGTAAG 
CTGCCAGCAC TGAGCAGCAG GAAACCTGAC GTTCCTAAAA CAAAGGTAGA AAAAAAAGAA 
CCAAGAGTTT CAGGATTTGG AATTAAGCAG TCGAAATTAG AGAGAAGAAA AGAAAAAAAG 
CAAACTGAAC AACACTGTGA AGTAGAAAAT GAAATTAACA GGAAAACGGA CAGCAGTGAC 
TTTTTAGATG ATGCTATGGA GTTTAAAATT TTGAAGCCAT CACGAAACAC GTCTGGCAAG 
ATTGGATGTG AACAACTCAG AGGTTCCTCC ATAGCTACAT ATTCATCAAA AGACAGTTTT 
ATGAAGGAAC TTCTAAACAG GAGTGTGTCG AAGGGCAGCT CCCAGGGCTC CTCTCTTCAC 
AGCAACTCTA TCAGCTCTCA AGGAAACCAC AAGAAAAACA GCAACACAAA AGCTGATATG 
GAAATACAGA ATCAGACCCT GGCTAACGTA GTCGCAGAAA ACCTGCAAGA AGAAGAGGAG 
GACACCATGA CAAGGACTAC ATGTCAGAGT CATGTGATAG AGTCTTGCTG CCAGATGAGG 
ACGTTGGACA GCGGGATTGG AACCTTCCCA CTCCCCGACT CAGGGAACCG ATCTGCGGGG 
CGACACGTGT CTAAGCAGGA ATTAACCTTG GAAACAGAAG CCCTAGCACC GTCTGAGCAA 
GTTCTTCTTT CAGCTCCTTC AGTAAAAGCC AAAACTCTTG AGCGAGAAGT GCCTTCAACA 
GCTACGAGTC AGGAGTCTGT GGAAAGCATG ATTAGTCACT CAACATCTGA TCCAGCCATG 
ACTGCCAAAG GTATACGACC TTTTCAAAGT CGCCTTCCAA AACCAGATTC CTCAGGAATA 
ATTAACCTTG CAAAGCAAAG TGAACAAGAA CCAAGTTCTG TGACCTCTAC CTCATTACAG 
CTTACTGAAG AAACTGTAGA AAATAGAAAA GTTCTTTCAG ATTGGACCAC CAAGAAAACT 
ACAAAAGCAA AGGACAGAAC TCTAAGAGTG TGCACTTACT CTGCCAGCAG CAGTGACGCT 
GAACCGGAGC CAGAGTATGG AACAAATTAT TTTCAAACTG CAGAGGAGAT GTTAGTAGAG 
TTAACAAAGA GCAACAAACA AGCTGAGCAG GAAAAACAGA AGCAGAGAAA GTCAGTTTTG 
GGGAACCCGA TGTCAATCTT GGATTTATAC CAGCACAGTC TCTATGGCCA TTTTGGAGAG 
GATGGACCTG AACAATTAAC ACATTTCAGT TTAATTGAGC AGCTGAGTGA ACCTTCCGGT 
AAAGACAGCA AGGACAAAGA GAGCCCAAGT AACCTGAAGC AAACTGAAGA AACAAAAGAA 
GATCCTCAGA CTAGATTATC TAAAATTTCC CTGGAGTCTC TCAATAAATT TAACAGCAAT 
AACGTCCTTT TATTAGAAAA GGAGAAGAAC TGTCTGAACA AAGCTGAAGG ACAAAAGGAA 
GAAAGCGGAA AGAAGGAAGC ATCTTCCTTA AACAGCTCCG ATAGGCAGGA CATGGACAAT 
TTGGAGTCCC TGAGCGATTC TTTGTACGAC AGCTTCTCCT CTTGTGCCAG CCAAGGCTCT 
AACGACGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_025152643.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025008411.1 Gallus gallus NCK associated protein 5 (NCKAP5), transcript variant X5, mRNA.

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CloneID	OGa152165
Clone ID Related Accession (Same CDS sequence)	XM_025152643.1
Accession Version	XM_025152643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	nck-associated protein 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAA AGAGACTACT ACAAAAAAGG GAAATTGGGA AAAGACTTTC TCTGGACAGC 
AGCCTTGTGG ACTATATGGA CTCAAACAAA TACATAGAGC ATCTAGTGAC ACAGCTAGAG 
GAACAGCGCT GGAATTTATG GAGGGAGAAG CTGTCTGTGG CTCGGCTGCA GCGAGAAGTT 
GCGCAGAGTA AGAGCGAAGG AACCATGCGC GAGAAACTGA TACATGAGTT GGAGGAAGAA 
AGACATTTAA GACTTGAGAG TGAAAAACGA TTACGGGAGG TAACACTGGA GTCAGAGCGT 
AACAGAGCTC AGATGCGTGG CCTACAAGAG CAGTTTTCCA GAATGGAGGA AACGGTGAGA 
AATCTACTAC AGAGCCAAGG ATCCCCAGGC CAGAATGGAG AAGGAACTGT CAATATCATG 
AAGGCATATC AGGAAAAGCT GTCAGAAGAC AGTAGGAAAT GCAAAGAAGT CATGGAAAAA 
AATCACACAG CAGTAGATGA AGATTCAAGA AATGAAAGCA ACAGCACTGA AGAGGAAAAA 
GAAAAGACAA AATTGCTACT GGAGCGTTTG AAAGCTCTGG AGGCAGAGAA TTCAGCACTG 
GCTCTGGAAA ATGAAAATCA GAGGGAACAG TATGAAAGAT GTCTTGATGA GGTAGCTAAT 
CAGGTCGTCC AGGCTCTACT TACTCAAAAG GATCTGAGGG AGGAATGTAT AAAGCTGAAA 
ACGAGAGTTT TTGATTTGGA GCAACAGAAT CAAACATTAA GTGTGCTTTT CCAGCAGAGA 
GTCAAACCAG CTTCTGACCT CCTCCTTCAG AAACTCCATT CGCGCATCTT AGACCTCTCA 
TCTGGAGATT TGCTGTCAGA AGTAGATCGA AACAGAAGCT CGGTGCCGTC ACGACCTGCT 
GATGCTCAGA TTCACGAATG CCAGCAGAAT GTGAAATCCA CTATACCTGT CTTGAAATGC 
CACAGTCAAT TAAATGTGAC AGGTCCTAGC CGCCTGTATC CCCGGAGCAG CTGCAGTAGC 
AGTGAACTCT CCCTTTCAAG TGCTTGCAGT GAATATTCCA GTGGTTCCTC CTACACTTGG 
AATGATGGGA AGACGTGCAG CAAAAGGTCA TCCGTGAGCT GGGATAAAAG AATAAGCATT 
GGTTCTTCAC TCCCAAGCAA CCTCTCTAGT CCTGCAGATG ATCTGCCTCC AACTCGTATC 
AAGGAAAACC ATATCCTAGA AGGACTGAAG AAATTACAGA GGCAAAAAGT ATTACTTGAA 
TCACCTTCAG TAATACCGAA ATGGGGTTAC AAAGATTGCA TGAACTCCAA TGAAGGAATA 
TATTCCCCTG GAGTTAAATG TGGCAGCCAT CATGAGCAGA CTCATTGTAA GCCAATGGAA 
ATAGGAAGTA GTTGCATTGA ACATAACAAA ACTTTCTCTT ATGATTCAGA CTCACATGAT 
GATGCAGATG ATGATTCTTC ATCTTTATTA TTGCTGCAGG AAGTACCAAG CAAAGACTGC 
AGGATCTATT GTAACAAATT AACTCACAGT GTTTCTGACA GTCTCTTTCA CTGGGATCCT 
AATAGGAAAC ATTTGCCAGA AAGAAGTTCA TATTTTAATT CAAAGGAAAG ACCAGAAAAA 
TTGACCAGTT TTGTCAGTGG ATTTCAGGCA GAAGTAGAAT CGTGCACCAG AACAAAGCTG 
CCTGATTTAC AGTTAGATCA AAGCCATGCT AATATAGGCT GGAAGGACCT TAATTTTCAG 
CTTTCAGATA CTGATGACAA TGAAGTCTTG GATGAATTGC ATATAGAAAG CAGTGATGAG 
AAAAGTCCAT CAGATTTATT AGCAACTCCT TTTACTGAGA AGTACACAAA GAACTCTGAT 
GTGCTCATAG TCACAGAGAA TTCTCAGTTT ATATCTAGTG ACAAAGAGGA AAGACAGACA 
TCTGCTCACT CAGACATTAG GCCAAAGACA TGTAATTTCA TTAAACAACA AAAAGTCATA 
AAAAAGACTT CTTCTGAGGA GTGCATTACA GTGATATTTG ATGCTGAGGA CGGTGAGCCT 
ATTGAATTCA GCTCGCATCA AATTGGAGTT GTCACTGTAA CAAGAAATGA GATATCAGTC 
AACCAACCAG AAAGTAGGTC CAGTGCAGAA TATACTGAAC TTATACCTCA GGGAATAGCT 
AATTTGCAGG CAGGAACAAA TGCTAGAGAC TACAGTGTTT TGGAAGGACC TGAAGACAAA 
ACTAAAAAAC AGACTCTTGA AGATAATACA GGGAATAAAA ATATGGTTGT CACCACAACC 
TGCAGTGAAT CTTCACCCTG TGGAAGAATC ATACTGCAAA ATGCACCACG ACAAAAACTA 
GTGAAGCCAG TGTATGATGT GTCGTACAAG ACCAATTCAA GCTCCATTGT TCATGCAGGA 
GTCAATCAAA AGCCAATCTT GACTAAAATA CCCAGCAGAG GTAAATTTTC TCCACAAAAA 
AACAAGATGA CAGAGAGTGA GGAGGCACCT GTAGCTACTT CAGTGACCCC TGCAACCCTT 
GAAAAATCAC CTGCTCTTCC ACCTGTAAAG CTTTCAAGAC TCAAAAAGGC ACAAAGTCCA 
CCTCGTAATT TTGACTCAAA AGTTGACTTC CAAGTTCCAA AGCCCCCTGC CCACCCCCTG 
CCCAGCTCAA AGCGTCCGAG CAGAGGCAAT GGGACAAAGT ATCCAAAGAG TCAGAGTCCA 
GCATCACCAC TGTTGCCTCA GCACCTTATA GAGCCTACTG ACTATGGAGA ACCTCCAACT 
AGAGACTCTA ATCATGATTT AGCAACTGTA GAAGCCAGGT CGCCATCCCC ACCCCTTCCT 
CCCGGCAGGT CAACGTCCCT ATTGATTCGT CCTGATTATG CTCATTCACA ACCTGCGGCT 
GTGAAGTTAG GAGGAGCTGT TCCAGGTGAA ACAGCAAAAA ATATACTGAA ACCATCACCA 
TTAAAAGGAA CTGCAAACTC TGGCATCCAT AATCAATCTA GTCATGATAT GCAAGCAAAA 
AGTCTATCTT CTGGGGCCAA AATTGAGTTA TCTTACCTTC AAGAAAATTC TGAAGCAATT 
ATGCAAAATA AATGTATATC TCAGCAACCC CATAGTGCAT GCCAAGGACT TTCCAAAGTT 
TCCACAAAGC AAGTCTGCCT GAAAAACACC AATCAGATGG GTCTCCACAG GAACCGTGAG 
TTTCTGAGCA TGGATTCTCA GACAGGCAGG TCCATTCCTC TGGGTCCGTC ACTGGAAACA 
ACTTCACAAG AAGTGCATTC CAGCAAGAAG GATATTTCCA GTGACAGTGG GGTTGATCAG 
CCACAAAAGA TGCCAAACAT TCTCCCTGCA ACTCCTCAAT GTCATACAGC AAGCGTGAGT 
GAGCCCTTGC TATCTCAGGT AAACAATTCT CCATTGTCAA CACTGTTTCA TGGTAGTGAC 
ACTGCAAATG CCACACAAAA CTCTAATGAG AAAGGAGTGA AAACCCGTCT CCCTGTAGGA 
CTGAAATTAT TTGTCAAATC TCCTCAACTT CTCCGAAAGA GCTCCACCGT CCCAGGGAAG 
CAGGAAAAAG ATAGTATAAA TGCAGCTTCC AAAAGCCACG TGAGTACAAG TAAGCACAAG 
CAAAATGAAC GTGTGGCCAC CAAAGGTGCA ATAGATGCTG AATTAAAGGA CTCTAAGTCT 
GATACCGAAA TAAAAGATAA TTTTTCTTTG AGACTTAGTG TGGATACAGC ATCACCTCGT 
TCACATGAAA ATGGCAGCTT GGTGGTAGAC AGAGTAGATG AATTAGAAAG CAAACTAGTT 
AAGAGATCTT TTTCTTCTAG CAACAAGTCA CACTTGAAAC CAGCTCTAGG CATGAATGGA 
GCGAAAGCTC GAAGTCAGAG TTTCAGCGTT CATACAGGAG AAAAGTCATC TACCCTTGTG 
ACAGAAGGTC TTGGAAAAGT ACGGACTCAG ATTATTACAA ACACTTCTGA CAGAGGAAAT 
TCTCTAACAA GGCAGAACTC AGCCATGGAA GGACTTCAAA TCAAGGCCAT TCCTGGGTCA 
GGAGCAACAC AAGAGACTCT TTCAAATACT TCAAAAAACA CAGAAAATGC TTACTCTAGA 
CAAGGTTCTC TTGGAAGTAA TAGCAATAAC CAGCATGGAA GCCCAAGCAA ACTGCCATTC 
AGATCATCTC CAAAAGTAGA CTTATTTCGA AACACTTCAA AATATGATGG AAACAAATCA 
TTTTCTATGA AAGATGCACG GAGTCTATCT GTCCAGAGTG AGAAAAGCAG TGAAAATTTA 
AAACACGAAG CTCTAAATAA AAAGAGTGTT TTGCCAGCAG AATTGGAGTT AGATACATCA 
GCTACTGTAT CTTCCAAACA GATTTCATCA ATTCATAGCT TGGGTGGAAT AAAATGTCCG 
CATAAACTGG AAGCAATAAA ATCACAGAGA CAGCAGATGT CTTTAAAGTT AGCAGAAGCC 
CCTCAGGTTA CTGATGTTTT TAGTTCACCT GCTCTGCAAC CTACAATAGA GGAGAAGGTC 
ATGTTGTGCA TCCAAGAAAA TGTCCAGAAG GGTCAGGAAC AAAACAAATC TCCCACTGTA 
GAGACTAAAC AAAAGACTGG GCCCTCTATA GCTAGTTGGT TTGGCTTTCG AAAGAGTAAG 
CTGCCAGCAC TGAGCAGCAG GAAACCTGAC GTTCCTAAAA CAAAGGTAGA AAAAAAAGAA 
CCAAGAGTTT CAGGATTTGG AATTAAGCAG TCGAAATTAG AGAGAAGAAA AGAAAAAAAG 
CAAACTGAAC AACACTGTGA AGTAGAAAAT GAAATTAACA GGAAAACGGA CAGCAGTGAC 
TTTTTAGATG ATGCTATGGA GTTTAAAATT TTGAAGCCAT CACGAAACAC GTCTGGCAAG 
ATTGGATGTG AACAACTCAG AGGTTCCTCC ATAGCTACAT ATTCATCAAA AGACAGTTTT 
ATGAAGGAAC TTCTAAACAG AGTTGATAAG AAAGCAGCTC AGCAGACAGA AAGTGGATCA 
AATAATGTTT CCTACAGGAG TGTGTCGAAG GGCAGCTCCC AGGGCTCCTC TCTTCACAGC 
AACTCTATCA GCTCTCAAGG AAACCACAAG AAAAACAGCA ACACAAAAGC TGATATGGAA 
ATACAGAATC AGACCCTGGC TAACGTAGTC GCAGAAAACC TGCAAGAAGA AGAGGAGGAC 
ACCATGACAA GGACTACATG TCAGAGTCAT GTGATAGAGT CTTGCTGCCA GATGAGGACG 
TTGGACAGCG GGATTGGAAC CTTCCCACTC CCCGACTCAG GGAACCGATC TGCGGGGCGA 
CACGTGTCTA AGCAGGAATT AACCTTGGAA ACAGAAGCCC TAGCACCGTC TGAGCAAGTT 
CTTCTTTCAG CTCCTTCAGT AAAAGCCAAA ACTCTTGAGC GAGAAGTGCC TTCAACAGCT 
ACGAGTCAGG AGTCTGTGGA AAGCATGATT AGTCACTCAA CATCTGATCC AGCCATGACT 
GCCAAAGGTA TACGACCTTT TCAAAGTCGC CTTCCAAAAC CAGATTCCTC AGGAATAATT 
AACCTTGCAA AGCAAAGTGA ACAAGAACCA AGTTCTGTGA CCTCTACCTC ATTACAGCTT 
ACTGAAGAAA CTGTAGAAAA TAGAAAAGTT CTTTCAGATT GGACCACCAA GAAAACTACA 
AAAGCAAAGG ACAGAACTCT AAGAGTGTGC ACTTACTCTG CCAGCAGCAG TGACGCTGAA 
CCGGAGCCAG AGTATGGAAC AAATTATTTT CAAACTGCAG AGGAGATGTT AGTAGAGTTA 
ACAAAGAGCA ACAAACAAGC TGAGCAGGAA AAACAGAAGC AGAGAAAGTC AGTTTTGGGG 
AACCCGATGT CAATCTTGGA TTTATACCAG CACAGTCTCT ATGGCCATTT TGGAGAGGAT 
GGACCTGAAC AATTAACACA TTTCAGTTTA ATTGAGCAGC TGAGTGAACC TTCCGGTAAA 
GACAGCAAGG ACAAAGAGAG CCCAAGTGTG AAGCAAGTGA AGCTTCTTTC ACTTCTGAGA 
GCTGTAGAAA AACAGCATGC AGCGGAGAGT GGTTCAGCAA CAAGGAGGGT GATAACATTT 
TCTATAACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025008411.1
CDS	287..6358
Translation	MERKRLLQKREIGKRLSLDSSLVDYMDSNKYIEHLVTQLEEQRWNLWREKLSVARLQREVAQSKSEGTMREKLIHELEEERHLRLESEKRLREVTLESERNRAQMRGLQEQFSRMEETVRNLLQSQGSPGQNGEGTVNIMKAYQEKLSEDSRKCKEVMEKNHTAVDEDSRNESNSTEEEKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECIKLKTRVFDLEQQNQTLSVLFQQRVKPASDLLLQKLHSRILDLSSGDLLSEVDRNRSSVPSRPADAQIHECQQNVKSTIPVLKCHSQLNVTGPSRLYPRSSCSSSELSLSSACSEYSSGSSYTWNDGKTCSKRSSVSWDKRISIGSSLPSNLSSPADDLPPTRIKENHILEGLKKLQRQKVLLESPSVIPKWGYKDCMNSNEGIYSPGVKCGSHHEQTHCKPMEIGSSCIEHNKTFSYDSDSHDDADDDSSSLLLLQEVPSKDCRIYCNKLTHSVSDSLFHWDPNRKHLPERSSYFNSKERPEKLTSFVSGFQAEVESCTRTKLPDLQLDQSHANIGWKDLNFQLSDTDDNEVLDELHIESSDEKSPSDLLATPFTEKYTKNSDVLIVTENSQFISSDKEERQTSAHSDIRPKTCNFIKQQKVIKKTSSEECITVIFDAEDGEPIEFSSHQIGVVTVTRNEISVNQPESRSSAEYTELIPQGIANLQAGTNARDYSVLEGPEDKTKKQTLEDNTGNKNMVVTTTCSESSPCGRIILQNAPRQKLVKPVYDVSYKTNSSSIVHAGVNQKPILTKIPSRGKFSPQKNKMTESEEAPVATSVTPATLEKSPALPPVKLSRLKKAQSPPRNFDSKVDFQVPKPPAHPLPSSKRPSRGNGTKYPKSQSPASPLLPQHLIEPTDYGEPPTRDSNHDLATVEARSPSPPLPPGRSTSLLIRPDYAHSQPAAVKLGGAVPGETAKNILKPSPLKGTANSGIHNQSSHDMQAKSLSSGAKIELSYLQENSEAIMQNKCISQQPHSACQGLSKVSTKQVCLKNTNQMGLHRNREFLSMDSQTGRSIPLGPSLETTSQEVHSSKKDISSDSGVDQPQKMPNILPATPQCHTASVSEPLLSQVNNSPLSTLFHGSDTANATQNSNEKGVKTRLPVGLKLFVKSPQLLRKSSTVPGKQEKDSINAASKSHVSTSKHKQNERVATKGAIDAELKDSKSDTEIKDNFSLRLSVDTASPRSHENGSLVVDRVDELESKLVKRSFSSSNKSHLKPALGMNGAKARSQSFSVHTGEKSSTLVTEGLGKVRTQIITNTSDRGNSLTRQNSAMEGLQIKAIPGSGATQETLSNTSKNTENAYSRQGSLGSNSNNQHGSPSKLPFRSSPKVDLFRNTSKYDGNKSFSMKDARSLSVQSEKSSENLKHEALNKKSVLPAELELDTSATVSSKQISSIHSLGGIKCPHKLEAIKSQRQQMSLKLAEAPQVTDVFSSPALQPTIEEKVMLCIQENVQKGQEQNKSPTVETKQKTGPSIASWFGFRKSKLPALSSRKPDVPKTKVEKKEPRVSGFGIKQSKLERRKEKKQTEQHCEVENEINRKTDSSDFLDDAMEFKILKPSRNTSGKIGCEQLRGSSIATYSSKDSFMKELLNRVDKKAAQQTESGSNNVSYRSVSKGSSQGSSLHSNSISSQGNHKKNSNTKADMEIQNQTLANVVAENLQEEEEDTMTRTTCQSHVIESCCQMRTLDSGIGTFPLPDSGNRSAGRHVSKQELTLETEALAPSEQVLLSAPSVKAKTLEREVPSTATSQESVESMISHSTSDPAMTAKGIRPFQSRLPKPDSSGIINLAKQSEQEPSSVTSTSLQLTEETVENRKVLSDWTTKKTTKAKDRTLRVCTYSASSSDAEPEPEYGTNYFQTAEEMLVELTKSNKQAEQEKQKQRKSVLGNPMSILDLYQHSLYGHFGEDGPEQLTHFSLIEQLSEPSGKDSKDKESPSVKQVKLLSLLRAVEKQHAAESGSATRRVITFSIT

Target ORF information:

RefSeq Version	XM_025152643.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NCK associated protein 5 (NCKAP5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025152643.1

ORF Insert Sequence:

ATGGAGAGAA AGAGACTACT ACAAAAAAGG GAAATTGGGA AAAGACTTTC TCTGGACAGC 
AGCCTTGTGG ACTATATGGA CTCAAACAAA TACATAGAGC ATCTAGTGAC ACAGCTAGAG 
GAACAGCGCT GGAATTTATG GAGGGAGAAG CTGTCTGTGG CTCGGCTGCA GCGAGAAGTT 
GCGCAGAGTA AGAGCGAAGG AACCATGCGC GAGAAACTGA TACATGAGTT GGAGGAAGAA 
AGACATTTAA GACTTGAGAG TGAAAAACGA TTACGGGAGG TAACACTGGA GTCAGAGCGT 
AACAGAGCTC AGATGCGTGG CCTACAAGAG CAGTTTTCCA GAATGGAGGA AACGGTGAGA 
AATCTACTAC AGAGCCAAGG ATCCCCAGGC CAGAATGGAG AAGGAACTGT CAATATCATG 
AAGGCATATC AGGAAAAGCT GTCAGAAGAC AGTAGGAAAT GCAAAGAAGT CATGGAAAAA 
AATCACACAG CAGTAGATGA AGATTCAAGA AATGAAAGCA ACAGCACTGA AGAGGAAAAA 
GAAAAGACAA AATTGCTACT GGAGCGTTTG AAAGCTCTGG AGGCAGAGAA TTCAGCACTG 
GCTCTGGAAA ATGAAAATCA GAGGGAACAG TATGAAAGAT GTCTTGATGA GGTAGCTAAT 
CAGGTCGTCC AGGCTCTACT TACTCAAAAG GATCTGAGGG AGGAATGTAT AAAGCTGAAA 
ACGAGAGTTT TTGATTTGGA GCAACAGAAT CAAACATTAA GTGTGCTTTT CCAGCAGAGA 
GTCAAACCAG CTTCTGACCT CCTCCTTCAG AAACTCCATT CGCGCATCTT AGACCTCTCA 
TCTGGAGATT TGCTGTCAGA AGTAGATCGA AACAGAAGCT CGGTGCCGTC ACGACCTGCT 
GATGCTCAGA TTCACGAATG CCAGCAGAAT GTGAAATCCA CTATACCTGT CTTGAAATGC 
CACAGTCAAT TAAATGTGAC AGGTCCTAGC CGCCTGTATC CCCGGAGCAG CTGCAGTAGC 
AGTGAACTCT CCCTTTCAAG TGCTTGCAGT GAATATTCCA GTGGTTCCTC CTACACTTGG 
AATGATGGGA AGACGTGCAG CAAAAGGTCA TCCGTGAGCT GGGATAAAAG AATAAGCATT 
GGTTCTTCAC TCCCAAGCAA CCTCTCTAGT CCTGCAGATG ATCTGCCTCC AACTCGTATC 
AAGGAAAACC ATATCCTAGA AGGACTGAAG AAATTACAGA GGCAAAAAGT ATTACTTGAA 
TCACCTTCAG TAATACCGAA ATGGGGTTAC AAAGATTGCA TGAACTCCAA TGAAGGAATA 
TATTCCCCTG GAGTTAAATG TGGCAGCCAT CATGAGCAGA CTCATTGTAA GCCAATGGAA 
ATAGGAAGTA GTTGCATTGA ACATAACAAA ACTTTCTCTT ATGATTCAGA CTCACATGAT 
GATGCAGATG ATGATTCTTC ATCTTTATTA TTGCTGCAGG AAGTACCAAG CAAAGACTGC 
AGGATCTATT GTAACAAATT AACTCACAGT GTTTCTGACA GTCTCTTTCA CTGGGATCCT 
AATAGGAAAC ATTTGCCAGA AAGAAGTTCA TATTTTAATT CAAAGGAAAG ACCAGAAAAA 
TTGACCAGTT TTGTCAGTGG ATTTCAGGCA GAAGTAGAAT CGTGCACCAG AACAAAGCTG 
CCTGATTTAC AGTTAGATCA AAGCCATGCT AATATAGGCT GGAAGGACCT TAATTTTCAG 
CTTTCAGATA CTGATGACAA TGAAGTCTTG GATGAATTGC ATATAGAAAG CAGTGATGAG 
AAAAGTCCAT CAGATTTATT AGCAACTCCT TTTACTGAGA AGTACACAAA GAACTCTGAT 
GTGCTCATAG TCACAGAGAA TTCTCAGTTT ATATCTAGTG ACAAAGAGGA AAGACAGACA 
TCTGCTCACT CAGACATTAG GCCAAAGACA TGTAATTTCA TTAAACAACA AAAAGTCATA 
AAAAAGACTT CTTCTGAGGA GTGCATTACA GTGATATTTG ATGCTGAGGA CGGTGAGCCT 
ATTGAATTCA GCTCGCATCA AATTGGAGTT GTCACTGTAA CAAGAAATGA GATATCAGTC 
AACCAACCAG AAAGTAGGTC CAGTGCAGAA TATACTGAAC TTATACCTCA GGGAATAGCT 
AATTTGCAGG CAGGAACAAA TGCTAGAGAC TACAGTGTTT TGGAAGGACC TGAAGACAAA 
ACTAAAAAAC AGACTCTTGA AGATAATACA GGGAATAAAA ATATGGTTGT CACCACAACC 
TGCAGTGAAT CTTCACCCTG TGGAAGAATC ATACTGCAAA ATGCACCACG ACAAAAACTA 
GTGAAGCCAG TGTATGATGT GTCGTACAAG ACCAATTCAA GCTCCATTGT TCATGCAGGA 
GTCAATCAAA AGCCAATCTT GACTAAAATA CCCAGCAGAG GTAAATTTTC TCCACAAAAA 
AACAAGATGA CAGAGAGTGA GGAGGCACCT GTAGCTACTT CAGTGACCCC TGCAACCCTT 
GAAAAATCAC CTGCTCTTCC ACCTGTAAAG CTTTCAAGAC TCAAAAAGGC ACAAAGTCCA 
CCTCGTAATT TTGACTCAAA AGTTGACTTC CAAGTTCCAA AGCCCCCTGC CCACCCCCTG 
CCCAGCTCAA AGCGTCCGAG CAGAGGCAAT GGGACAAAGT ATCCAAAGAG TCAGAGTCCA 
GCATCACCAC TGTTGCCTCA GCACCTTATA GAGCCTACTG ACTATGGAGA ACCTCCAACT 
AGAGACTCTA ATCATGATTT AGCAACTGTA GAAGCCAGGT CGCCATCCCC ACCCCTTCCT 
CCCGGCAGGT CAACGTCCCT ATTGATTCGT CCTGATTATG CTCATTCACA ACCTGCGGCT 
GTGAAGTTAG GAGGAGCTGT TCCAGGTGAA ACAGCAAAAA ATATACTGAA ACCATCACCA 
TTAAAAGGAA CTGCAAACTC TGGCATCCAT AATCAATCTA GTCATGATAT GCAAGCAAAA 
AGTCTATCTT CTGGGGCCAA AATTGAGTTA TCTTACCTTC AAGAAAATTC TGAAGCAATT 
ATGCAAAATA AATGTATATC TCAGCAACCC CATAGTGCAT GCCAAGGACT TTCCAAAGTT 
TCCACAAAGC AAGTCTGCCT GAAAAACACC AATCAGATGG GTCTCCACAG GAACCGTGAG 
TTTCTGAGCA TGGATTCTCA GACAGGCAGG TCCATTCCTC TGGGTCCGTC ACTGGAAACA 
ACTTCACAAG AAGTGCATTC CAGCAAGAAG GATATTTCCA GTGACAGTGG GGTTGATCAG 
CCACAAAAGA TGCCAAACAT TCTCCCTGCA ACTCCTCAAT GTCATACAGC AAGCGTGAGT 
GAGCCCTTGC TATCTCAGGT AAACAATTCT CCATTGTCAA CACTGTTTCA TGGTAGTGAC 
ACTGCAAATG CCACACAAAA CTCTAATGAG AAAGGAGTGA AAACCCGTCT CCCTGTAGGA 
CTGAAATTAT TTGTCAAATC TCCTCAACTT CTCCGAAAGA GCTCCACCGT CCCAGGGAAG 
CAGGAAAAAG ATAGTATAAA TGCAGCTTCC AAAAGCCACG TGAGTACAAG TAAGCACAAG 
CAAAATGAAC GTGTGGCCAC CAAAGGTGCA ATAGATGCTG AATTAAAGGA CTCTAAGTCT 
GATACCGAAA TAAAAGATAA TTTTTCTTTG AGACTTAGTG TGGATACAGC ATCACCTCGT 
TCACATGAAA ATGGCAGCTT GGTGGTAGAC AGAGTAGATG AATTAGAAAG CAAACTAGTT 
AAGAGATCTT TTTCTTCTAG CAACAAGTCA CACTTGAAAC CAGCTCTAGG CATGAATGGA 
GCGAAAGCTC GAAGTCAGAG TTTCAGCGTT CATACAGGAG AAAAGTCATC TACCCTTGTG 
ACAGAAGGTC TTGGAAAAGT ACGGACTCAG ATTATTACAA ACACTTCTGA CAGAGGAAAT 
TCTCTAACAA GGCAGAACTC AGCCATGGAA GGACTTCAAA TCAAGGCCAT TCCTGGGTCA 
GGAGCAACAC AAGAGACTCT TTCAAATACT TCAAAAAACA CAGAAAATGC TTACTCTAGA 
CAAGGTTCTC TTGGAAGTAA TAGCAATAAC CAGCATGGAA GCCCAAGCAA ACTGCCATTC 
AGATCATCTC CAAAAGTAGA CTTATTTCGA AACACTTCAA AATATGATGG AAACAAATCA 
TTTTCTATGA AAGATGCACG GAGTCTATCT GTCCAGAGTG AGAAAAGCAG TGAAAATTTA 
AAACACGAAG CTCTAAATAA AAAGAGTGTT TTGCCAGCAG AATTGGAGTT AGATACATCA 
GCTACTGTAT CTTCCAAACA GATTTCATCA ATTCATAGCT TGGGTGGAAT AAAATGTCCG 
CATAAACTGG AAGCAATAAA ATCACAGAGA CAGCAGATGT CTTTAAAGTT AGCAGAAGCC 
CCTCAGGTTA CTGATGTTTT TAGTTCACCT GCTCTGCAAC CTACAATAGA GGAGAAGGTC 
ATGTTGTGCA TCCAAGAAAA TGTCCAGAAG GGTCAGGAAC AAAACAAATC TCCCACTGTA 
GAGACTAAAC AAAAGACTGG GCCCTCTATA GCTAGTTGGT TTGGCTTTCG AAAGAGTAAG 
CTGCCAGCAC TGAGCAGCAG GAAACCTGAC GTTCCTAAAA CAAAGGTAGA AAAAAAAGAA 
CCAAGAGTTT CAGGATTTGG AATTAAGCAG TCGAAATTAG AGAGAAGAAA AGAAAAAAAG 
CAAACTGAAC AACACTGTGA AGTAGAAAAT GAAATTAACA GGAAAACGGA CAGCAGTGAC 
TTTTTAGATG ATGCTATGGA GTTTAAAATT TTGAAGCCAT CACGAAACAC GTCTGGCAAG 
ATTGGATGTG AACAACTCAG AGGTTCCTCC ATAGCTACAT ATTCATCAAA AGACAGTTTT 
ATGAAGGAAC TTCTAAACAG AGTTGATAAG AAAGCAGCTC AGCAGACAGA AAGTGGATCA 
AATAATGTTT CCTACAGGAG TGTGTCGAAG GGCAGCTCCC AGGGCTCCTC TCTTCACAGC 
AACTCTATCA GCTCTCAAGG AAACCACAAG AAAAACAGCA ACACAAAAGC TGATATGGAA 
ATACAGAATC AGACCCTGGC TAACGTAGTC GCAGAAAACC TGCAAGAAGA AGAGGAGGAC 
ACCATGACAA GGACTACATG TCAGAGTCAT GTGATAGAGT CTTGCTGCCA GATGAGGACG 
TTGGACAGCG GGATTGGAAC CTTCCCACTC CCCGACTCAG GGAACCGATC TGCGGGGCGA 
CACGTGTCTA AGCAGGAATT AACCTTGGAA ACAGAAGCCC TAGCACCGTC TGAGCAAGTT 
CTTCTTTCAG CTCCTTCAGT AAAAGCCAAA ACTCTTGAGC GAGAAGTGCC TTCAACAGCT 
ACGAGTCAGG AGTCTGTGGA AAGCATGATT AGTCACTCAA CATCTGATCC AGCCATGACT 
GCCAAAGGTA TACGACCTTT TCAAAGTCGC CTTCCAAAAC CAGATTCCTC AGGAATAATT 
AACCTTGCAA AGCAAAGTGA ACAAGAACCA AGTTCTGTGA CCTCTACCTC ATTACAGCTT 
ACTGAAGAAA CTGTAGAAAA TAGAAAAGTT CTTTCAGATT GGACCACCAA GAAAACTACA 
AAAGCAAAGG ACAGAACTCT AAGAGTGTGC ACTTACTCTG CCAGCAGCAG TGACGCTGAA 
CCGGAGCCAG AGTATGGAAC AAATTATTTT CAAACTGCAG AGGAGATGTT AGTAGAGTTA 
ACAAAGAGCA ACAAACAAGC TGAGCAGGAA AAACAGAAGC AGAGAAAGTC AGTTTTGGGG 
AACCCGATGT CAATCTTGGA TTTATACCAG CACAGTCTCT ATGGCCATTT TGGAGAGGAT 
GGACCTGAAC AATTAACACA TTTCAGTTTA ATTGAGCAGC TGAGTGAACC TTCCGGTAAA 
GACAGCAAGG ACAAAGAGAG CCCAAGTGTG AAGCAAGTGA AGCTTCTTTC ACTTCTGAGA 
GCTGTAGAAA AACAGCATGC AGCGGAGAGT GGTTCAGCAA CAAGGAGGGT GATAACATTT 
TCTATAACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_015289926.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145412.1 Gallus gallus NCK-associated protein 5 (NCKAP5), transcript variant X1, mRNA.

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CloneID	OGa38635
Clone ID Related Accession (Same CDS sequence)	XM_015289926.1
Accession Version	XM_015289926.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	nck-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAC AACTCTTTGA CCTCGGGCTG ACGAGGCGGG CTCTCCAGAA GCAATCTTCC 
CAGCACGGCA GGATTTACGA GGCACGCCAG CCGGCCAGAC CCAACGGTGC GCTGCCCCCT 
CAGCAGGCCG CGTCTCCTTC CCACGCTTCC GACGGCCCCG AGGCGGCACC TCCGCTCGGG 
GAGCGCCGCG GGCCGTACTG TGCGGCGCTG CCCCCGGGGG ATTTCGGCGG CGGGAGAGCC 
TCGCCAGGGG CAGCCCCGGC CCGGGACGAG CCGCCGGCCC CGGCCCCGCC GCCGCAGACA 
ATGGCCGCCC CCGCCCGACC CCCCCCCCAA CCCCCTCCCC GCCGCGGCGA GCCCCTGCTG 
CCGCCGCACG GTTGCCGGAG CCATTCAGTC GCGGCCGATC GCGACCGCGG CAGATGGGCA 
GGATCTGACG GCCCGGTGCT CCGCGGCTGC CCGGGGCCGG GGCGGGCGGC TCGGGCGGAG 
CCCCCCCCCC TCGCCCCCGC GCGAAGTGCC GCCCCCGCAT GCCGAGCGTC CCGCGGCCGC 
GGAGGGAGCG GGGCACTCCG CAGCGCGACG AAGCGGAGGA GGCGCGCGGC GGCCGGCGGC 
GGGGGGCAGC CCGGGGGGCG CGGCCCCGCA CCGCTCGTCG AGGCGGCGGC CGGCGCCGCG 
CTCCCCGGGC CCGGCGCGGG CCGCCCCCGG CCGCCGCAGC ATGCCCCGCC GGGCAGCAGG 
GAGAAGCTGT CTGTGGCTCG GCTGCAGCGA GAAGTTGCGC AGAGTAAGAG CGAAGGAACC 
ATGCGCGAGA AACTGATACA TGAGTTGGAG GAAGAAAGAC ATTTAAGACT TGAGAGTGAA 
AAACGATTAC GGGAGGTAAC ACTGGAGTCA GAGCGTAACA GAGCTCAGAT GCGTGGCCTA 
CAAGAGCAGT TTTCCAGAAT GGAGGAAACG GTGAGAAATC TACTACAGAG CCAAGGATCC 
CCAGGCCAGA ATGGAGAAGG AACTGTCAAT ATCATGAAGG CATATCAGGA AAAGCTGTCA 
GAAGACAGTA GGAAATGCAA AGAAGTCATG GAAAAAAATC ACACAGCAGT AGATGAAGAT 
TCAAGAAATG AAAGCAACAG CACTGAAGAG GAAAAAGAAA AGACAAAATT GCTACTGGAG 
CGTTTGAAAG CTCTGGAGGC AGAGAATTCA GCACTGGCTC TGGAAAATGA AAATCAGAGG 
GAACAGTATG AAAGATGTCT TGATGAGGTA GCTAATCAGG TCGTCCAGGC TCTACTTACT 
CAAAAGGATC TGAGGGAGGA ATGTATAAAG CTGAAAACGA GAGTTTTTGA TTTGGAGCAA 
CAGAATCAAA CATTAAGTGT GCTTTTCCAG CAGAGAGTCA AACCAGCTTC TGACCTCCTC 
CTTCAGAAAC TCCATTCGCG CATCTTAGAC CTCTCATCTG GAGATTTGCT GTCAGAAGTA 
GATCGAAACA GAAGCTCGGT GCCGTCACGA CCTGCTGATG CTCAGATTCA CGAATGCCAG 
CAGAATGTGA AATCCACTAT ACCTGTCTTG AAATGCCACA GTCAATTAAA TGTGACAGGT 
CCTAGCCGCC TGTATCCCCG GAGCAGCTGC AGTAGCAGTG AACTCTCCCT TTCAAGTGCT 
TGCAGTGAAT ATTCCAGTGG TTCCTCCTAC ACTTGGAATG ATGGGAAGAC GTGCAGCAAA 
AGGTCATCCG TGAGCTGGGA TAAAAGAATA AGCATTGGTT CTTCACTCCC AAGCAACCTC 
TCTAGTCCTG CAGATGATCT GCCTCCAACT CGTATCAAGG AAAACCATAT CCTAGAAGGA 
CTGAAGAAAT TACAGAGGCA AAAAGTATTA CTTGAATCAC CTTCAGTAAT ACCGAAATGG 
GGTTACAAAG ATTGCATGAA CTCCAATGAA GGAATATATT CCCCTGGAGT TAAATGTGGC 
AGCCATCATG AGCAGACTCA TTGTAAGCCA ATGGAAATAG GAAGTAGTTG CATTGAACAT 
AACAAAACTT TCTCTTATGA TTCAGACTCA CATGATGATG CAGATGATGA TTCTTCATCT 
TTATTATTGC TGCAGGAAGT ACCAAGCAAA GACTGCAGGA TCTATTGTAA CAAATTAACT 
CACAGTGTTT CTGACAGTCT CTTTCACTGG GATCCTAATA GGAAACATTT GCCAGAAAGA 
AGTTCATATT TTAATTCAAA GGAAAGACCA GAAAAATTGA CCAGTTTTGT CAGTGGATTT 
CAGGCAGAAG TAGAATCGTG CACCAGAACA AAGCTGCCTG ATTTACAGTT AGATCAAAGC 
CATGCTAATA TAGGCTGGAA GGACCTTAAT TTTCAGCTTT CAGATACTGA TGACAATGAA 
GTCTTGGATG AATTGCATAT AGAAAGCAGT GATGAGAAAA GTCCATCAGA TTTATTAGCA 
ACTCCTTTTA CTGAGAAGTA CACAAAGAAC TCTGATGTGC TCATAGTCAC AGAGAATTCT 
CAGTTTATAT CTAGTGACAA AGAGGAAAGA CAGACATCTG CTCACTCAGA CATTAGGCCA 
AAGACATGTA ATTTCATTAA ACAACAAAAA GTCATAAAAA AGACTTCTTC TGAGGAGTGC 
ATTACAGTGA TATTTGATGC TGAGGACGGT GAGCCTATTG AATTCAGCTC GCATCAAATT 
GGAGTTGTCA CTGTAACAAG AAATGAGATA TCAGTCAACC AACCAGAAAG TAGGTCCAGT 
GCAGAATATA CTGAACTTAT ACCTCAGGGA ATAGCTAATT TGCAGGCAGG AACAAATGCT 
AGAGACTACA GTGTTTTGGA AGGACCTGAA GACAAAACTA AAAAACAGAC TCTTGAAGAT 
AATACAGGGA ATAAAAATAT GGTTGTCACC ACAACCTGCA GTGAATCTTC ACCCTGTGGA 
AGAATCATAC TGCAAAATGC ACCACGACAA AAACTAGTGA AGCCAGTGTA TGATGTGTCG 
TACAAGACCA ATTCAAGCTC CATTGTTCAT GCAGGAGTCA ATCAAAAGCC AATCTTGACT 
AAAATACCCA GCAGAGGTAA ATTTTCTCCA CAAAAAAACA AGATGACAGA GAGTGAGGAG 
GCACCTGTAG CTACTTCAGT GACCCCTGCA ACCCTTGAAA AATCACCTGC TCTTCCACCT 
GTAAAGCTTT CAAGACTCAA AAAGGCACAA AGTCCACCTC GTAATTTTGA CTCAAAAGTT 
GACTTCCAAG TTCCAAAGCC CCCTGCCCAC CCCCTGCCCA GCTCAAAGCG TCCGAGCAGA 
GGCAATGGGA CAAAGTATCC AAAGAGTCAG AGTCCAGCAT CACCACTGTT GCCTCAGCAC 
CTTATAGAGC CTACTGACTA TGGAGAACCT CCAACTAGAG ACTCTAATCA TGATTTAGCA 
ACTGTAGAAG CCAGGTCGCC ATCCCCACCC CTTCCTCCCG GCAGGTCAAC GTCCCTATTG 
ATTCGTCCTG ATTATGCTCA TTCACAACCT GCGGCTGTGA AGTTAGGAGG AGCTGTTCCA 
GGTGAAACAG CAAAAAATAT ACTGAAACCA TCACCATTAA AAGGAACTGC AAACTCTGGC 
ATCCATAATC AATCTAGTCA TGATATGCAA GCAAAAAGTC TATCTTCTGG GGCCAAAATT 
GAGTTATCTT ACCTTCAAGA AAATTCTGAA GCAATTATGC AAAATAAATG TATATCTCAG 
CAACCCCATA GTGCATGCCA AGGACTTTCC AAAGTTTCCA CAAAGCAAGT CTGCCTGAAA 
AACACCAATC AGATGGGTCT CCACAGGAAC CGTGAGTTTC TGAGCATGGA TTCTCAGACA 
GGCAGGTCCA TTCCTCTGGG TCCGTCACTG GAAACAACTT CACAAGAAGT GCATTCCAGC 
AAGAAGGATA TTTCCAGTGA CAGTGGGGTT GATCAGCCAC AAAAGATGCC AAACATTCTC 
CCTGCAACTC CTCAATGTCA TACAGCAAGC GTGAGTGAGC CCTTGCTATC TCAGGTAAAC 
AATTCTCCAT TGTCAACACT GTTTCATGGT AGTGACACTG CAAATGCCAC ACAAAACTCT 
AATGAGAAAG GAGTGAAAAC CCGTCTCCCT GTAGGACTGA AATTATTTGT CAAATCTCCT 
CAACTTCTCC GAAAGAGCTC CACCGTCCCA GGGAAGCAGG AAAAAGATAG TATAAATGCA 
GCTTCCAAAA GCCACGTGAG TACAAGTAAG CACAAGCAAA ATGAACGTGT GGCCACCAAA 
GGTGCAATAG ATGCTGAATT AAAGGACTCT AAGTCTGATA CCGAAATAAA AGATAATTTT 
TCTTTGAGAC TTAGTGTGGA TACAGCATCA CCTCGTTCAC ATGAAAATGG CAGCTTGGTG 
GTAGACAGAG TAGATGAATT AGAAAGCAAA CTAGTTAAGA GATCTTTTTC TTCTAGCAAC 
AAGTCACACT TGAAACCAGC TCTAGGCATG AATGGAGCGA AAGCTCGAAG TCAGAGTTTC 
AGCGTTCATA CAGGAGAAAA GTCATCTACC CTTGTGACAG AAGGTCTTGG AAAAGTACGG 
ACTCAGATTA TTACAAACAC TTCTGACAGA GGAAATTCTC TAACAAGGCA GAACTCAGCC 
ATGGAAGGAC TTCAAATCAA GGCCATTCCT GGGTCAGGAG CAACACAAGA GACTCTTTCA 
AATACTTCAA AAAACACAGA AAATGCTTAC TCTAGACAAG GTTCTCTTGG AAGTAATAGC 
AATAACCAGC ATGGAAGCCC AAGCAAACTG CCATTCAGAT CATCTCCAAA AGTAGACTTA 
TTTCGAAACA CTTCAAAATA TGATGGAAAC AAATCATTTT CTATGAAAGA TGCACGGAGT 
CTATCTGTCC AGAGTGAGAA AAGCAGTGAA AATTTAAAAC ACGAAGCTCT AAATAAAAAG 
AGTGTTTTGC CAGCAGAATT GGAGTTAGAT ACATCAGCTA CTGTATCTTC CAAACAGATT 
TCATCAATTC ATAGCTTGGG TGGAATAAAA TGTCCGCATA AACTGGAAGC AATAAAATCA 
CAGAGACAGC AGATGTCTTT AAAGTTAGCA GAAGCCCCTC AGGTTACTGA TGTTTTTAGT 
TCACCTGCTC TGCAACCTAC AATAGAGGAG AAGGTCATGT TGTGCATCCA AGAAAATGTC 
CAGAAGGGTC AGGAACAAAA CAAATCTCCC ACTGTAGAGA CTAAACAAAA GACTGGGCCC 
TCTATAGCTA GTTGGTTTGG CTTTCGAAAG AGTAAGCTGC CAGCACTGAG CAGCAGGAAA 
CCTGACGTTC CTAAAACAAA GGTAGAAAAA AAAGAACCAA GAGTTTCAGG ATTTGGAATT 
AAGCAGTCGA AATTAGAGAG AAGAAAAGAA AAAAAGCAAA CTGAACAACA CTGTGAAGTA 
GAAAATGAAA TTAACAGGAA AACGGACAGC AGTGACTTTT TAGATGATGC TATGGAGTTT 
AAAATTTTGA AGCCATCACG AAACACGTCT GGCAAGATTG GATGTGAACA ACTCAGAGGT 
TCCTCCATAG CTACATATTC ATCAAAAGAC AGTTTTATGA AGGAACTTCT AAACAGAGTT 
GATAAGAAAG CAGCTCAGCA GACAGAAAGT GGATCAAATA ATGTTTCCTA CAGGAGTGTG 
TCGAAGGGCA GCTCCCAGGG CTCCTCTCTT CACAGCAACT CTATCAGCTC TCAAGGAAAC 
CACAAGAAAA ACAGCAACAC AAAAGCTGAT ATGGAAATAC AGAATCAGAC CCTGGCTAAC 
GTAGTCGCAG AAAACCTGCA AGAAGAAGAG GAGGACACCA TGACAAGGAC TACATGTCAG 
AGTCATGTGA TAGAGTCTTG CTGCCAGATG AGGACGTTGG ACAGCGGGAT TGGAACCTTC 
CCACTCCCCG ACTCAGGGAA CCGATCTGCG GGGCGACACG TGTCTAAGCA GGAATTAACC 
TTGGAAACAG AAGCCCTAGC ACCGTCTGAG CAAGTTCTTC TTTCAGCTCC TTCAGTAAAA 
GCCAAAACTC TTGAGCGAGA AGTGCCTTCA ACAGCTACGA GTCAGGAGTC TGTGGAAAGC 
ATGATTAGTC ACTCAACATC TGATCCAGCC ATGACTGCCA AAGGTATACG ACCTTTTCAA 
AGTCGCCTTC CAAAACCAGA TTCCTCAGGA ATAATTAACC TTGCAAAGCA AAGTGAACAA 
GAACCAAGTT CTGTGACCTC TACCTCATTA CAGCTTACTG AAGAAACTGT AGAAAATAGA 
AAAGTTCTTT CAGATTGGAC CACCAAGAAA ACTACAAAAG CAAAGGACAG AACTCTAAGA 
GTGTGCACTT ACTCTGCCAG CAGCAGTGAC GCTGAACCGG AGCCAGAGTA TGGAACAAAT 
TATTTTCAAA CTGCAGAGGA GATGTTAGTA GAGTTAACAA AGAGCAACAA ACAAGCTGAG 
CAGGAAAAAC AGAAGCAGAG AAAGTCAGTT TTGGGGAACC CGATGTCAAT CTTGGATTTA 
TACCAGCACA GTCTCTATGG CCATTTTGGA GAGGATGGAC CTGAACAATT AACACATTTC 
AGTTTAATTG AGCAGCTGAG TGAACCTTCC GGTAAAGACA GCAAGGACAA AGAGAGCCCA 
AGTAACCTGA AGCAAACTGA AGAAACAAAA GAAGATCCTC AGACTAGATT ATCTAAAATT 
TCCCTGGAGT CTCTCAATAA ATTTAACAGC AATAACGTCC TTTTATTAGA AAAGGAGAAG 
AACTGTCTGA ACAAAGCTGA AGGACAAAAG GAAGAAAGCG GAAAGAAGGA AGCATCTTCC 
TTAAACAGCT CCGATAGGCA GGACATGGAC AATTTGGAGT CCCTGAGCGA TTCTTTGTAC 
GACAGCTTCT CCTCTTGTGC CAGCCAAGGC TCTAACGACG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145412.1
CDS	5358..12182
Translation	MATQLFDLGLTRRALQKQSSQHGRIYEARQPARPNGALPPQQAASPSHASDGPEAAPPLGERRGPYCAALPPGDFGGGRASPGAAPARDEPPAPAPPPQTMAAPARPPPQPPPRRGEPLLPPHGCRSHSVAADRDRGRWAGSDGPVLRGCPGPGRAARAEPPPLAPARSAAPACRASRGRGGSGALRSATKRRRRAAAGGGGQPGGRGPAPLVEAAAGAALPGPGAGRPRPPQHAPPGSREKLSVARLQREVAQSKSEGTMREKLIHELEEERHLRLESEKRLREVTLESERNRAQMRGLQEQFSRMEETVRNLLQSQGSPGQNGEGTVNIMKAYQEKLSEDSRKCKEVMEKNHTAVDEDSRNESNSTEEEKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECIKLKTRVFDLEQQNQTLSVLFQQRVKPASDLLLQKLHSRILDLSSGDLLSEVDRNRSSVPSRPADAQIHECQQNVKSTIPVLKCHSQLNVTGPSRLYPRSSCSSSELSLSSACSEYSSGSSYTWNDGKTCSKRSSVSWDKRISIGSSLPSNLSSPADDLPPTRIKENHILEGLKKLQRQKVLLESPSVIPKWGYKDCMNSNEGIYSPGVKCGSHHEQTHCKPMEIGSSCIEHNKTFSYDSDSHDDADDDSSSLLLLQEVPSKDCRIYCNKLTHSVSDSLFHWDPNRKHLPERSSYFNSKERPEKLTSFVSGFQAEVESCTRTKLPDLQLDQSHANIGWKDLNFQLSDTDDNEVLDELHIESSDEKSPSDLLATPFTEKYTKNSDVLIVTENSQFISSDKEERQTSAHSDIRPKTCNFIKQQKVIKKTSSEECITVIFDAEDGEPIEFSSHQIGVVTVTRNEISVNQPESRSSAEYTELIPQGIANLQAGTNARDYSVLEGPEDKTKKQTLEDNTGNKNMVVTTTCSESSPCGRIILQNAPRQKLVKPVYDVSYKTNSSSIVHAGVNQKPILTKIPSRGKFSPQKNKMTESEEAPVATSVTPATLEKSPALPPVKLSRLKKAQSPPRNFDSKVDFQVPKPPAHPLPSSKRPSRGNGTKYPKSQSPASPLLPQHLIEPTDYGEPPTRDSNHDLATVEARSPSPPLPPGRSTSLLIRPDYAHSQPAAVKLGGAVPGETAKNILKPSPLKGTANSGIHNQSSHDMQAKSLSSGAKIELSYLQENSEAIMQNKCISQQPHSACQGLSKVSTKQVCLKNTNQMGLHRNREFLSMDSQTGRSIPLGPSLETTSQEVHSSKKDISSDSGVDQPQKMPNILPATPQCHTASVSEPLLSQVNNSPLSTLFHGSDTANATQNSNEKGVKTRLPVGLKLFVKSPQLLRKSSTVPGKQEKDSINAASKSHVSTSKHKQNERVATKGAIDAELKDSKSDTEIKDNFSLRLSVDTASPRSHENGSLVVDRVDELESKLVKRSFSSSNKSHLKPALGMNGAKARSQSFSVHTGEKSSTLVTEGLGKVRTQIITNTSDRGNSLTRQNSAMEGLQIKAIPGSGATQETLSNTSKNTENAYSRQGSLGSNSNNQHGSPSKLPFRSSPKVDLFRNTSKYDGNKSFSMKDARSLSVQSEKSSENLKHEALNKKSVLPAELELDTSATVSSKQISSIHSLGGIKCPHKLEAIKSQRQQMSLKLAEAPQVTDVFSSPALQPTIEEKVMLCIQENVQKGQEQNKSPTVETKQKTGPSIASWFGFRKSKLPALSSRKPDVPKTKVEKKEPRVSGFGIKQSKLERRKEKKQTEQHCEVENEINRKTDSSDFLDDAMEFKILKPSRNTSGKIGCEQLRGSSIATYSSKDSFMKELLNRVDKKAAQQTESGSNNVSYRSVSKGSSQGSSLHSNSISSQGNHKKNSNTKADMEIQNQTLANVVAENLQEEEEDTMTRTTCQSHVIESCCQMRTLDSGIGTFPLPDSGNRSAGRHVSKQELTLETEALAPSEQVLLSAPSVKAKTLEREVPSTATSQESVESMISHSTSDPAMTAKGIRPFQSRLPKPDSSGIINLAKQSEQEPSSVTSTSLQLTEETVENRKVLSDWTTKKTTKAKDRTLRVCTYSASSSDAEPEPEYGTNYFQTAEEMLVELTKSNKQAEQEKQKQRKSVLGNPMSILDLYQHSLYGHFGEDGPEQLTHFSLIEQLSEPSGKDSKDKESPSNLKQTEETKEDPQTRLSKISLESLNKFNSNNVLLLEKEKNCLNKAEGQKEESGKKEASSLNSSDRQDMDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_015289926.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NCK-associated protein 5 (NCKAP5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289926.1

ORF Insert Sequence:

ATGGCCACAC AACTCTTTGA CCTCGGGCTG ACGAGGCGGG CTCTCCAGAA GCAATCTTCC 
CAGCACGGCA GGATTTACGA GGCACGCCAG CCGGCCAGAC CCAACGGTGC GCTGCCCCCT 
CAGCAGGCCG CGTCTCCTTC CCACGCTTCC GACGGCCCCG AGGCGGCACC TCCGCTCGGG 
GAGCGCCGCG GGCCGTACTG TGCGGCGCTG CCCCCGGGGG ATTTCGGCGG CGGGAGAGCC 
TCGCCAGGGG CAGCCCCGGC CCGGGACGAG CCGCCGGCCC CGGCCCCGCC GCCGCAGACA 
ATGGCCGCCC CCGCCCGACC CCCCCCCCAA CCCCCTCCCC GCCGCGGCGA GCCCCTGCTG 
CCGCCGCACG GTTGCCGGAG CCATTCAGTC GCGGCCGATC GCGACCGCGG CAGATGGGCA 
GGATCTGACG GCCCGGTGCT CCGCGGCTGC CCGGGGCCGG GGCGGGCGGC TCGGGCGGAG 
CCCCCCCCCC TCGCCCCCGC GCGAAGTGCC GCCCCCGCAT GCCGAGCGTC CCGCGGCCGC 
GGAGGGAGCG GGGCACTCCG CAGCGCGACG AAGCGGAGGA GGCGCGCGGC GGCCGGCGGC 
GGGGGGCAGC CCGGGGGGCG CGGCCCCGCA CCGCTCGTCG AGGCGGCGGC CGGCGCCGCG 
CTCCCCGGGC CCGGCGCGGG CCGCCCCCGG CCGCCGCAGC ATGCCCCGCC GGGCAGCAGG 
GAGAAGCTGT CTGTGGCTCG GCTGCAGCGA GAAGTTGCGC AGAGTAAGAG CGAAGGAACC 
ATGCGCGAGA AACTGATACA TGAGTTGGAG GAAGAAAGAC ATTTAAGACT TGAGAGTGAA 
AAACGATTAC GGGAGGTAAC ACTGGAGTCA GAGCGTAACA GAGCTCAGAT GCGTGGCCTA 
CAAGAGCAGT TTTCCAGAAT GGAGGAAACG GTGAGAAATC TACTACAGAG CCAAGGATCC 
CCAGGCCAGA ATGGAGAAGG AACTGTCAAT ATCATGAAGG CATATCAGGA AAAGCTGTCA 
GAAGACAGTA GGAAATGCAA AGAAGTCATG GAAAAAAATC ACACAGCAGT AGATGAAGAT 
TCAAGAAATG AAAGCAACAG CACTGAAGAG GAAAAAGAAA AGACAAAATT GCTACTGGAG 
CGTTTGAAAG CTCTGGAGGC AGAGAATTCA GCACTGGCTC TGGAAAATGA AAATCAGAGG 
GAACAGTATG AAAGATGTCT TGATGAGGTA GCTAATCAGG TCGTCCAGGC TCTACTTACT 
CAAAAGGATC TGAGGGAGGA ATGTATAAAG CTGAAAACGA GAGTTTTTGA TTTGGAGCAA 
CAGAATCAAA CATTAAGTGT GCTTTTCCAG CAGAGAGTCA AACCAGCTTC TGACCTCCTC 
CTTCAGAAAC TCCATTCGCG CATCTTAGAC CTCTCATCTG GAGATTTGCT GTCAGAAGTA 
GATCGAAACA GAAGCTCGGT GCCGTCACGA CCTGCTGATG CTCAGATTCA CGAATGCCAG 
CAGAATGTGA AATCCACTAT ACCTGTCTTG AAATGCCACA GTCAATTAAA TGTGACAGGT 
CCTAGCCGCC TGTATCCCCG GAGCAGCTGC AGTAGCAGTG AACTCTCCCT TTCAAGTGCT 
TGCAGTGAAT ATTCCAGTGG TTCCTCCTAC ACTTGGAATG ATGGGAAGAC GTGCAGCAAA 
AGGTCATCCG TGAGCTGGGA TAAAAGAATA AGCATTGGTT CTTCACTCCC AAGCAACCTC 
TCTAGTCCTG CAGATGATCT GCCTCCAACT CGTATCAAGG AAAACCATAT CCTAGAAGGA 
CTGAAGAAAT TACAGAGGCA AAAAGTATTA CTTGAATCAC CTTCAGTAAT ACCGAAATGG 
GGTTACAAAG ATTGCATGAA CTCCAATGAA GGAATATATT CCCCTGGAGT TAAATGTGGC 
AGCCATCATG AGCAGACTCA TTGTAAGCCA ATGGAAATAG GAAGTAGTTG CATTGAACAT 
AACAAAACTT TCTCTTATGA TTCAGACTCA CATGATGATG CAGATGATGA TTCTTCATCT 
TTATTATTGC TGCAGGAAGT ACCAAGCAAA GACTGCAGGA TCTATTGTAA CAAATTAACT 
CACAGTGTTT CTGACAGTCT CTTTCACTGG GATCCTAATA GGAAACATTT GCCAGAAAGA 
AGTTCATATT TTAATTCAAA GGAAAGACCA GAAAAATTGA CCAGTTTTGT CAGTGGATTT 
CAGGCAGAAG TAGAATCGTG CACCAGAACA AAGCTGCCTG ATTTACAGTT AGATCAAAGC 
CATGCTAATA TAGGCTGGAA GGACCTTAAT TTTCAGCTTT CAGATACTGA TGACAATGAA 
GTCTTGGATG AATTGCATAT AGAAAGCAGT GATGAGAAAA GTCCATCAGA TTTATTAGCA 
ACTCCTTTTA CTGAGAAGTA CACAAAGAAC TCTGATGTGC TCATAGTCAC AGAGAATTCT 
CAGTTTATAT CTAGTGACAA AGAGGAAAGA CAGACATCTG CTCACTCAGA CATTAGGCCA 
AAGACATGTA ATTTCATTAA ACAACAAAAA GTCATAAAAA AGACTTCTTC TGAGGAGTGC 
ATTACAGTGA TATTTGATGC TGAGGACGGT GAGCCTATTG AATTCAGCTC GCATCAAATT 
GGAGTTGTCA CTGTAACAAG AAATGAGATA TCAGTCAACC AACCAGAAAG TAGGTCCAGT 
GCAGAATATA CTGAACTTAT ACCTCAGGGA ATAGCTAATT TGCAGGCAGG AACAAATGCT 
AGAGACTACA GTGTTTTGGA AGGACCTGAA GACAAAACTA AAAAACAGAC TCTTGAAGAT 
AATACAGGGA ATAAAAATAT GGTTGTCACC ACAACCTGCA GTGAATCTTC ACCCTGTGGA 
AGAATCATAC TGCAAAATGC ACCACGACAA AAACTAGTGA AGCCAGTGTA TGATGTGTCG 
TACAAGACCA ATTCAAGCTC CATTGTTCAT GCAGGAGTCA ATCAAAAGCC AATCTTGACT 
AAAATACCCA GCAGAGGTAA ATTTTCTCCA CAAAAAAACA AGATGACAGA GAGTGAGGAG 
GCACCTGTAG CTACTTCAGT GACCCCTGCA ACCCTTGAAA AATCACCTGC TCTTCCACCT 
GTAAAGCTTT CAAGACTCAA AAAGGCACAA AGTCCACCTC GTAATTTTGA CTCAAAAGTT 
GACTTCCAAG TTCCAAAGCC CCCTGCCCAC CCCCTGCCCA GCTCAAAGCG TCCGAGCAGA 
GGCAATGGGA CAAAGTATCC AAAGAGTCAG AGTCCAGCAT CACCACTGTT GCCTCAGCAC 
CTTATAGAGC CTACTGACTA TGGAGAACCT CCAACTAGAG ACTCTAATCA TGATTTAGCA 
ACTGTAGAAG CCAGGTCGCC ATCCCCACCC CTTCCTCCCG GCAGGTCAAC GTCCCTATTG 
ATTCGTCCTG ATTATGCTCA TTCACAACCT GCGGCTGTGA AGTTAGGAGG AGCTGTTCCA 
GGTGAAACAG CAAAAAATAT ACTGAAACCA TCACCATTAA AAGGAACTGC AAACTCTGGC 
ATCCATAATC AATCTAGTCA TGATATGCAA GCAAAAAGTC TATCTTCTGG GGCCAAAATT 
GAGTTATCTT ACCTTCAAGA AAATTCTGAA GCAATTATGC AAAATAAATG TATATCTCAG 
CAACCCCATA GTGCATGCCA AGGACTTTCC AAAGTTTCCA CAAAGCAAGT CTGCCTGAAA 
AACACCAATC AGATGGGTCT CCACAGGAAC CGTGAGTTTC TGAGCATGGA TTCTCAGACA 
GGCAGGTCCA TTCCTCTGGG TCCGTCACTG GAAACAACTT CACAAGAAGT GCATTCCAGC 
AAGAAGGATA TTTCCAGTGA CAGTGGGGTT GATCAGCCAC AAAAGATGCC AAACATTCTC 
CCTGCAACTC CTCAATGTCA TACAGCAAGC GTGAGTGAGC CCTTGCTATC TCAGGTAAAC 
AATTCTCCAT TGTCAACACT GTTTCATGGT AGTGACACTG CAAATGCCAC ACAAAACTCT 
AATGAGAAAG GAGTGAAAAC CCGTCTCCCT GTAGGACTGA AATTATTTGT CAAATCTCCT 
CAACTTCTCC GAAAGAGCTC CACCGTCCCA GGGAAGCAGG AAAAAGATAG TATAAATGCA 
GCTTCCAAAA GCCACGTGAG TACAAGTAAG CACAAGCAAA ATGAACGTGT GGCCACCAAA 
GGTGCAATAG ATGCTGAATT AAAGGACTCT AAGTCTGATA CCGAAATAAA AGATAATTTT 
TCTTTGAGAC TTAGTGTGGA TACAGCATCA CCTCGTTCAC ATGAAAATGG CAGCTTGGTG 
GTAGACAGAG TAGATGAATT AGAAAGCAAA CTAGTTAAGA GATCTTTTTC TTCTAGCAAC 
AAGTCACACT TGAAACCAGC TCTAGGCATG AATGGAGCGA AAGCTCGAAG TCAGAGTTTC 
AGCGTTCATA CAGGAGAAAA GTCATCTACC CTTGTGACAG AAGGTCTTGG AAAAGTACGG 
ACTCAGATTA TTACAAACAC TTCTGACAGA GGAAATTCTC TAACAAGGCA GAACTCAGCC 
ATGGAAGGAC TTCAAATCAA GGCCATTCCT GGGTCAGGAG CAACACAAGA GACTCTTTCA 
AATACTTCAA AAAACACAGA AAATGCTTAC TCTAGACAAG GTTCTCTTGG AAGTAATAGC 
AATAACCAGC ATGGAAGCCC AAGCAAACTG CCATTCAGAT CATCTCCAAA AGTAGACTTA 
TTTCGAAACA CTTCAAAATA TGATGGAAAC AAATCATTTT CTATGAAAGA TGCACGGAGT 
CTATCTGTCC AGAGTGAGAA AAGCAGTGAA AATTTAAAAC ACGAAGCTCT AAATAAAAAG 
AGTGTTTTGC CAGCAGAATT GGAGTTAGAT ACATCAGCTA CTGTATCTTC CAAACAGATT 
TCATCAATTC ATAGCTTGGG TGGAATAAAA TGTCCGCATA AACTGGAAGC AATAAAATCA 
CAGAGACAGC AGATGTCTTT AAAGTTAGCA GAAGCCCCTC AGGTTACTGA TGTTTTTAGT 
TCACCTGCTC TGCAACCTAC AATAGAGGAG AAGGTCATGT TGTGCATCCA AGAAAATGTC 
CAGAAGGGTC AGGAACAAAA CAAATCTCCC ACTGTAGAGA CTAAACAAAA GACTGGGCCC 
TCTATAGCTA GTTGGTTTGG CTTTCGAAAG AGTAAGCTGC CAGCACTGAG CAGCAGGAAA 
CCTGACGTTC CTAAAACAAA GGTAGAAAAA AAAGAACCAA GAGTTTCAGG ATTTGGAATT 
AAGCAGTCGA AATTAGAGAG AAGAAAAGAA AAAAAGCAAA CTGAACAACA CTGTGAAGTA 
GAAAATGAAA TTAACAGGAA AACGGACAGC AGTGACTTTT TAGATGATGC TATGGAGTTT 
AAAATTTTGA AGCCATCACG AAACACGTCT GGCAAGATTG GATGTGAACA ACTCAGAGGT 
TCCTCCATAG CTACATATTC ATCAAAAGAC AGTTTTATGA AGGAACTTCT AAACAGAGTT 
GATAAGAAAG CAGCTCAGCA GACAGAAAGT GGATCAAATA ATGTTTCCTA CAGGAGTGTG 
TCGAAGGGCA GCTCCCAGGG CTCCTCTCTT CACAGCAACT CTATCAGCTC TCAAGGAAAC 
CACAAGAAAA ACAGCAACAC AAAAGCTGAT ATGGAAATAC AGAATCAGAC CCTGGCTAAC 
GTAGTCGCAG AAAACCTGCA AGAAGAAGAG GAGGACACCA TGACAAGGAC TACATGTCAG 
AGTCATGTGA TAGAGTCTTG CTGCCAGATG AGGACGTTGG ACAGCGGGAT TGGAACCTTC 
CCACTCCCCG ACTCAGGGAA CCGATCTGCG GGGCGACACG TGTCTAAGCA GGAATTAACC 
TTGGAAACAG AAGCCCTAGC ACCGTCTGAG CAAGTTCTTC TTTCAGCTCC TTCAGTAAAA 
GCCAAAACTC TTGAGCGAGA AGTGCCTTCA ACAGCTACGA GTCAGGAGTC TGTGGAAAGC 
ATGATTAGTC ACTCAACATC TGATCCAGCC ATGACTGCCA AAGGTATACG ACCTTTTCAA 
AGTCGCCTTC CAAAACCAGA TTCCTCAGGA ATAATTAACC TTGCAAAGCA AAGTGAACAA 
GAACCAAGTT CTGTGACCTC TACCTCATTA CAGCTTACTG AAGAAACTGT AGAAAATAGA 
AAAGTTCTTT CAGATTGGAC CACCAAGAAA ACTACAAAAG CAAAGGACAG AACTCTAAGA 
GTGTGCACTT ACTCTGCCAG CAGCAGTGAC GCTGAACCGG AGCCAGAGTA TGGAACAAAT 
TATTTTCAAA CTGCAGAGGA GATGTTAGTA GAGTTAACAA AGAGCAACAA ACAAGCTGAG 
CAGGAAAAAC AGAAGCAGAG AAAGTCAGTT TTGGGGAACC CGATGTCAAT CTTGGATTTA 
TACCAGCACA GTCTCTATGG CCATTTTGGA GAGGATGGAC CTGAACAATT AACACATTTC 
AGTTTAATTG AGCAGCTGAG TGAACCTTCC GGTAAAGACA GCAAGGACAA AGAGAGCCCA 
AGTAACCTGA AGCAAACTGA AGAAACAAAA GAAGATCCTC AGACTAGATT ATCTAAAATT 
TCCCTGGAGT CTCTCAATAA ATTTAACAGC AATAACGTCC TTTTATTAGA AAAGGAGAAG 
AACTGTCTGA ACAAAGCTGA AGGACAAAAG GAAGAAAGCG GAAAGAAGGA AGCATCTTCC 
TTAAACAGCT CCGATAGGCA GGACATGGAC AATTTGGAGT CCCTGAGCGA TTCTTTGTAC 
GACAGCTTCT CCTCTTGTGC CAGCCAAGGC TCTAACGACG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_015289927.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145413.1 Gallus gallus NCK-associated protein 5 (NCKAP5), transcript variant X2, mRNA.

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CloneID	OGa38636
Clone ID Related Accession (Same CDS sequence)	XM_015289927.1
Accession Version	XM_015289927.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	nck-associated protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAC AACTCTTTGA CCTCGGGCTG ACGAGGCGGG CTCTCCAGAA GCAATCTTCC 
CAGCACGGCA GGATTTACGA GGCACGCCAG CCGGCCAGAC CCAACGGTGC GCTGCCCCCT 
CAGCAGGCCG CGTCTCCTTC CCACGCTTCC GACGGCCCCG AGGCGGCACC TCCGCTCGGG 
GAGCGCCGCG GGCCGTACTG TGCGGCGCTG CCCCCGGGGG ATTTCGGCGG CGGGAGAGCC 
TCGCCAGGGG CAGCCCCGGC CCGGGACGAG CCGCCGGCCC CGGCCCCGCC GCCGCAGACA 
ATGGCCGCCC CCGCCCGACC CCCCCCCCAA CCCCCTCCCC GCCGCGGCGA GCCCCTGCTG 
CCGCCGCACG GTTGCCGGAG CCATTCAGTC GCGGCCGATC GCGACCGCGG CAGATGGGCA 
GGATCTGACG GCCCGGTGCT CCGCGGCTGC CCGGGGCCGG GGCGGGCGGC TCGGGCGGAG 
CCCCCCCCCC TCGCCCCCGC GCGAAGTGCC GCCCCCGCAT GCCGAGCGTC CCGCGGCCGC 
GGAGGGAGCG GGGCACTCCG CAGCGCGACG AAGCGGAGGA GGCGCGCGGC GGCCGGCGGC 
GGGGGGCAGC CCGGGGGGCG CGGCCCCGCA CCGCTCGTCG AGGCGGCGGC CGGCGCCGCG 
CTCCCCGGGC CCGGCGCGGG CCGCCCCCGG CCGCCGCAGC ATGCCCCGCC GGGCAGCAGG 
GAGAAGCTGT CTGTGGCTCG GCTGCAGCGA GAAGTTGCGC AGAGTAAGAG CGAAGGAACC 
ATGCGCGAGA AACTGATACA TGAGTTGGAG GAAGAAAGAC ATTTAAGACT TGAGAGTGAA 
AAACGATTAC GGGAGGTAAC ACTGGAGTCA GAGCGTAACA GAGCTCAGAT GCGTGGCCTA 
CAAGAGCAGT TTTCCAGAAT GGAGGAAACG GTGAGAAATC TACTACAGAG CCAAGGATCC 
CCAGGCCAGA ATGGAGAAGG AACTGTCAAT ATCATGAAGG CATATCAGGA AAAGCTGTCA 
GAAGACAGTA GGAAATGCAA AGAAGTCATG GAAAAAAATC ACACAGCAGT AGATGAAGAT 
TCAAGAAATG AAAGCAACAG CACTGAAGAG GAAAAAGAAA AGACAAAATT GCTACTGGAG 
CGTTTGAAAG CTCTGGAGGC AGAGAATTCA GCACTGGCTC TGGAAAATGA AAATCAGAGG 
GAACAGTATG AAAGATGTCT TGATGAGGTA GCTAATCAGG TCGTCCAGGC TCTACTTACT 
CAAAAGGATC TGAGGGAGGA ATGTATAAAG CTGAAAACGA GAGTTTTTGA TTTGGAGCAA 
CAGAATCAAA CATTAAGTGT GCTTTTCCAG CAGAGAGTCA AACCAGCTTC TGACCTCCTC 
CTTCAGAAAC TCCATTCGCG CATCTTAGAC CTCTCATCTG GAGATTTGCT GTCAGAAGTA 
GATCGAAACA GAAGCTCGGT GCCGTCACGA CCTGCTGATG CTCAGATTCA CGAATGCCAG 
CAGAATGTGA AATCCACTAT ACCTGTCTTG AAATGCCACA GTCAATTAAA TGTGACAGGT 
CCTAGCCGCC TGTATCCCCG GAGCAGCTGC AGTAGCAGTG AACTCTCCCT TTCAAGTGCT 
TGCAGTGAAT ATTCCAGTGG TTCCTCCTAC ACTTGGAATG ATGGGAAGAC GTGCAGCAAA 
AGGTCATCCG TGAGCTGGGA TAAAAGAATA AGCATTGGTT CTTCACTCCC AAGCAACCTC 
TCTAGTCCTG CAGATGATCT GCCTCCAACT CGTATCAAGG AAAACCATAT CCTAGAAGGA 
CTGAAGAAAT TACAGAGGCA AAAAGTATTA CTTGAATCAC CTTCAGTAAT ACCGAAATGG 
GGTTACAAAG ATTGCATGAA CTCCAATGAA GGAATATATT CCCCTGGAGT TAAATGTGGC 
AGCCATCATG AGCAGACTCA TTGTAAGCCA ATGGAAATAG GAAGTAGTTG CATTGAACAT 
AACAAAACTT TCTCTTATGA TTCAGACTCA CATGATGATG CAGATGATGA TTCTTCATCT 
TTATTATTGC TGCAGGAAGT ACCAAGCAAA GACTGCAGGA TCTATTGTAA CAAATTAACT 
CACAGTGTTT CTGACAGTCT CTTTCACTGG GATCCTAATA GGAAACATTT GCCAGAAAGA 
AGTTCATATT TTAATTCAAA GGAAAGACCA GAAAAATTGA CCAGTTTTGT CAGTGGATTT 
CAGGCAGAAG TAGAATCGTG CACCAGAACA AAGCTGCCTG ATTTACAGTT AGATCAAAGC 
CATGCTAATA TAGGCTGGAA GGACCTTAAT TTTCAGCTTT CAGATACTGA TGACAATGAA 
GTCTTGGATG AATTGCATAT AGAAAGCAGT GATGAGAAAA GTCCATCAGA TTTATTAGCA 
ACTCCTTTTA CTGAGAAGTA CACAAAGAAC TCTGATGTGC TCATAGTCAC AGAGAATTCT 
CAGTTTATAT CTAGTGACAA AGAGGAAAGA CAGACATCTG CTCACTCAGA CATTAGGCCA 
AAGACATGTA ATTTCATTAA ACAACAAAAA GTCATAAAAA AGACTTCTTC TGAGGAGTGC 
ATTACAGTGA TATTTGATGC TGAGGACGGT GAGCCTATTG AATTCAGCTC GCATCAAATT 
GGAGTTGTCA CTGTAACAAG AAATGAGATA TCAGTCAACC AACCAGAAAG TAGGTCCAGT 
GCAGAATATA CTGAACTTAT ACCTCAGGGA ATAGCTAATT TGCAGGCAGG AACAAATGCT 
AGAGACTACA GTGTTTTGGA AGGACCTGAA GACAAAACTA AAAAACAGAC TCTTGAAGAT 
AATACAGGGA ATAAAAATAT GGTTGTCACC ACAACCTGCA GTGAATCTTC ACCCTGTGGA 
AGAATCATAC TGCAAAATGC ACCACGACAA AAACTAGTGA AGCCAGTGTA TGATGTGTCG 
TACAAGACCA ATTCAAGCTC CATTGTTCAT GCAGGAGTCA ATCAAAAGCC AATCTTGACT 
AAAATACCCA GCAGAGGTAA ATTTTCTCCA CAAAAAAACA AGATGACAGA GAGTGAGGAG 
GCACCTGTAG CTACTTCAGT GACCCCTGCA ACCCTTGAAA AATCACCTGC TCTTCCACCT 
GTAAAGCTTT CAAGACTCAA AAAGGCACAA AGTCCACCTC GTAATTTTGA CTCAAAAGTT 
GACTTCCAAG TTCCAAAGCC CCCTGCCCAC CCCCTGCCCA GCTCAAAGCG TCCGAGCAGA 
GGCAATGGGA CAAAGTATCC AAAGAGTCAG AGTCCAGCAT CACCACTGTT GCCTCAGCAC 
CTTATAGAGC CTACTGACTA TGGAGAACCT CCAACTAGAG ACTCTAATCA TGATTTAGCA 
ACTGTAGAAG CCAGGTCGCC ATCCCCACCC CTTCCTCCCG GCAGGTCAAC GTCCCTATTG 
ATTCGTCCTG ATTATGCTCA TTCACAACCT GCGGCTGTGA AGTTAGGAGG AGCTGTTCCA 
GGTGAAACAG CAAAAAATAT ACTGAAACCA TCACCATTAA AAGGAACTGC AAACTCTGGC 
ATCCATAATC AATCTAGTCA TGATATGCAA GCAAAAAGTC TATCTTCTGG GGCCAAAATT 
GAGTTATCTT ACCTTCAAGA AAATTCTGAA GCAATTATGC AAAATAAATG TATATCTCAG 
CAACCCCATA GTGCATGCCA AGGACTTTCC AAAGTTTCCA CAAAGCAAGT CTGCCTGAAA 
AACACCAATC AGATGGGTCT CCACAGGAAC CGTGAGTTTC TGAGCATGGA TTCTCAGACA 
GGCAGGTCCA TTCCTCTGGG TCCGTCACTG GAAACAACTT CACAAGAAGT GCATTCCAGC 
AAGAAGGATA TTTCCAGTGA CAGTGGGGTT GATCAGCCAC AAAAGATGCC AAACATTCTC 
CCTGCAACTC CTCAATGTCA TACAGCAAGC GTGAGTGAGC CCTTGCTATC TCAGGTAAAC 
AATTCTCCAT TGTCAACACT GTTTCATGGT AGTGACACTG CAAATGCCAC ACAAAACTCT 
AATGAGAAAG GAGTGAAAAC CCGTCTCCCT GTAGGACTGA AATTATTTGT CAAATCTCCT 
CAACTTCTCC GAAAGAGCTC CACCGTCCCA GGGAAGCAGG AAAAAGATAG TATAAATGCA 
GCTTCCAAAA GCCACGTGAG TACAAGTAAG CACAAGCAAA ATGAACGTGT GGCCACCAAA 
GGTGCAATAG ATGCTGAATT AAAGGACTCT AAGTCTGATA CCGAAATAAA AGATAATTTT 
TCTTTGAGAC TTAGTGTGGA TACAGCATCA CCTCGTTCAC ATGAAAATGG CAGCTTGGTG 
GTAGACAGAG TAGATGAATT AGAAAGCAAA CTAGTTAAGA GATCTTTTTC TTCTAGCAAC 
AAGTCACACT TGAAACCAGC TCTAGGCATG AATGGAGCGA AAGCTCGAAG TCAGAGTTTC 
AGCGTTCATA CAGGAGAAAA GTCATCTACC CTTGTGACAG AAGGTCTTGG AAAAGTACGG 
ACTCAGATTA TTACAAACAC TTCTGACAGA GGAAATTCTC TAACAAGGCA GAACTCAGCC 
ATGGAAGGAC TTCAAATCAA GGCCATTCCT GGGTCAGGAG CAACACAAGA GACTCTTTCA 
AATACTTCAA AAAACACAGA AAATGCTTAC TCTAGACAAG GTTCTCTTGG AAGTAATAGC 
AATAACCAGC ATGGAAGCCC AAGCAAACTG CCATTCAGAT CATCTCCAAA AGTAGACTTA 
TTTCGAAACA CTTCAAAATA TGATGGAAAC AAATCATTTT CTATGAAAGA TGCACGGAGT 
CTATCTGTCC AGAGTGAGAA AAGCAGTGAA AATTTAAAAC ACGAAGCTCT AAATAAAAAG 
AGTGTTTTGC CAGCAGAATT GGAGTTAGAT ACATCAGCTA CTGTATCTTC CAAACAGATT 
TCATCAATTC ATAGCTTGGG TGGAATAAAA TGTCCGCATA AACTGGAAGC AATAAAATCA 
CAGAGACAGC AGATGTCTTT AAAGTTAGCA GAAGCCCCTC AGGTTACTGA TGTTTTTAGT 
TCACCTGCTC TGCAACCTAC AATAGAGGAG AAGGTCATGT TGTGCATCCA AGAAAATGTC 
CAGAAGGGTC AGGAACAAAA CAAATCTCCC ACTGTAGAGA CTAAACAAAA GACTGGGCCC 
TCTATAGCTA GTTGGTTTGG CTTTCGAAAG AGTAAGCTGC CAGCACTGAG CAGCAGGAAA 
CCTGACGTTC CTAAAACAAA GGTAGAAAAA AAAGAACCAA GAGTTTCAGG ATTTGGAATT 
AAGCAGTCGA AATTAGAGAG AAGAAAAGAA AAAAAGCAAA CTGAACAACA CTGTGAAGTA 
GAAAATGAAA TTAACAGGAA AACGGACAGC AGTGACTTTT TAGATGATGC TATGGAGTTT 
AAAATTTTGA AGCCATCACG AAACACGTCT GGCAAGATTG GATGTGAACA ACTCAGAGGT 
TCCTCCATAG CTACATATTC ATCAAAAGAC AGTTTTATGA AGGAACTTCT AAACAGAGTT 
GATAAGAAAG CAGCTCAGCA GACAGAAAGT GGATCAAATA ATGTTTCCTA CAGGAGTGTG 
TCGAAGGGCA GCTCCCAGGG CTCCTCTCTT CACAGCAACT CTATCAGCTC TCAAGGAAAC 
CACAAGAAAA ACAGCAACAC AAAAGCTGAT ATGGAAATAC AGAATCAGAC CCTGGCTAAC 
GTAGTCGCAG AAAACCTGCA AGAAGAAGAG GAGGACACCA TGACAAGGAC TACATGTCAG 
AGTCATGTGA TAGAGTCTTG CTGCCAGATG AGGACGTTGG ACAGCGGGAT TGGAACCTTC 
CCACTCCCCG ACTCAGGGAA CCGATCTGCG GGGCGACACG TGTCTAAGCA GGAATTAACC 
TTGGAAACAG AAGCCCTAGC ACCGTCTGAG CAAGTTCTTC TTTCAGCTCC TTCAGTAAAA 
GCCAAAACTC TTGAGCGAGA AGTGCCTTCA ACAGCTACGA GTCAGGAGTC TGTGGAAAGC 
ATGATTAGTC ACTCAACATC TGATCCAGCC ATGACTGCCA AAGGAATAAT TAACCTTGCA 
AAGCAAAGTG AACAAGAACC AAGTTCTGTG ACCTCTACCT CATTACAGCT TACTGAAGAA 
ACTGTAGAAA ATAGAAAAGT TCTTTCAGAT TGGACCACCA AGAAAACTAC AAAAGCAAAG 
GACAGAACTC TAAGAGTGTG CACTTACTCT GCCAGCAGCA GTGACGCTGA ACCGGAGCCA 
GAGTATGGAA CAAATTATTT TCAAACTGCA GAGGAGATGT TAGTAGAGTT AACAAAGAGC 
AACAAACAAG CTGAGCAGGA AAAACAGAAG CAGAGAAAGT CAGTTTTGGG GAACCCGATG 
TCAATCTTGG ATTTATACCA GCACAGTCTC TATGGCCATT TTGGAGAGGA TGGACCTGAA 
CAATTAACAC ATTTCAGTTT AATTGAGCAG CTGAGTGAAC CTTCCGGTAA AGACAGCAAG 
GACAAAGAGA GCCCAAGTAA CCTGAAGCAA ACTGAAGAAA CAAAAGAAGA TCCTCAGACT 
AGATTATCTA AAATTTCCCT GGAGTCTCTC AATAAATTTA ACAGCAATAA CGTCCTTTTA 
TTAGAAAAGG AGAAGAACTG TCTGAACAAA GCTGAAGGAC AAAAGGAAGA AAGCGGAAAG 
AAGGAAGCAT CTTCCTTAAA CAGCTCCGAT AGGCAGGACA TGGACAATTT GGAGTCCCTG 
AGCGATTCTT TGTACGACAG CTTCTCCTCT TGTGCCAGCC AAGGCTCTAA CGACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145413.1
CDS	5360..12139
Translation	MATQLFDLGLTRRALQKQSSQHGRIYEARQPARPNGALPPQQAASPSHASDGPEAAPPLGERRGPYCAALPPGDFGGGRASPGAAPARDEPPAPAPPPQTMAAPARPPPQPPPRRGEPLLPPHGCRSHSVAADRDRGRWAGSDGPVLRGCPGPGRAARAEPPPLAPARSAAPACRASRGRGGSGALRSATKRRRRAAAGGGGQPGGRGPAPLVEAAAGAALPGPGAGRPRPPQHAPPGSREKLSVARLQREVAQSKSEGTMREKLIHELEEERHLRLESEKRLREVTLESERNRAQMRGLQEQFSRMEETVRNLLQSQGSPGQNGEGTVNIMKAYQEKLSEDSRKCKEVMEKNHTAVDEDSRNESNSTEEEKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECIKLKTRVFDLEQQNQTLSVLFQQRVKPASDLLLQKLHSRILDLSSGDLLSEVDRNRSSVPSRPADAQIHECQQNVKSTIPVLKCHSQLNVTGPSRLYPRSSCSSSELSLSSACSEYSSGSSYTWNDGKTCSKRSSVSWDKRISIGSSLPSNLSSPADDLPPTRIKENHILEGLKKLQRQKVLLESPSVIPKWGYKDCMNSNEGIYSPGVKCGSHHEQTHCKPMEIGSSCIEHNKTFSYDSDSHDDADDDSSSLLLLQEVPSKDCRIYCNKLTHSVSDSLFHWDPNRKHLPERSSYFNSKERPEKLTSFVSGFQAEVESCTRTKLPDLQLDQSHANIGWKDLNFQLSDTDDNEVLDELHIESSDEKSPSDLLATPFTEKYTKNSDVLIVTENSQFISSDKEERQTSAHSDIRPKTCNFIKQQKVIKKTSSEECITVIFDAEDGEPIEFSSHQIGVVTVTRNEISVNQPESRSSAEYTELIPQGIANLQAGTNARDYSVLEGPEDKTKKQTLEDNTGNKNMVVTTTCSESSPCGRIILQNAPRQKLVKPVYDVSYKTNSSSIVHAGVNQKPILTKIPSRGKFSPQKNKMTESEEAPVATSVTPATLEKSPALPPVKLSRLKKAQSPPRNFDSKVDFQVPKPPAHPLPSSKRPSRGNGTKYPKSQSPASPLLPQHLIEPTDYGEPPTRDSNHDLATVEARSPSPPLPPGRSTSLLIRPDYAHSQPAAVKLGGAVPGETAKNILKPSPLKGTANSGIHNQSSHDMQAKSLSSGAKIELSYLQENSEAIMQNKCISQQPHSACQGLSKVSTKQVCLKNTNQMGLHRNREFLSMDSQTGRSIPLGPSLETTSQEVHSSKKDISSDSGVDQPQKMPNILPATPQCHTASVSEPLLSQVNNSPLSTLFHGSDTANATQNSNEKGVKTRLPVGLKLFVKSPQLLRKSSTVPGKQEKDSINAASKSHVSTSKHKQNERVATKGAIDAELKDSKSDTEIKDNFSLRLSVDTASPRSHENGSLVVDRVDELESKLVKRSFSSSNKSHLKPALGMNGAKARSQSFSVHTGEKSSTLVTEGLGKVRTQIITNTSDRGNSLTRQNSAMEGLQIKAIPGSGATQETLSNTSKNTENAYSRQGSLGSNSNNQHGSPSKLPFRSSPKVDLFRNTSKYDGNKSFSMKDARSLSVQSEKSSENLKHEALNKKSVLPAELELDTSATVSSKQISSIHSLGGIKCPHKLEAIKSQRQQMSLKLAEAPQVTDVFSSPALQPTIEEKVMLCIQENVQKGQEQNKSPTVETKQKTGPSIASWFGFRKSKLPALSSRKPDVPKTKVEKKEPRVSGFGIKQSKLERRKEKKQTEQHCEVENEINRKTDSSDFLDDAMEFKILKPSRNTSGKIGCEQLRGSSIATYSSKDSFMKELLNRVDKKAAQQTESGSNNVSYRSVSKGSSQGSSLHSNSISSQGNHKKNSNTKADMEIQNQTLANVVAENLQEEEEDTMTRTTCQSHVIESCCQMRTLDSGIGTFPLPDSGNRSAGRHVSKQELTLETEALAPSEQVLLSAPSVKAKTLEREVPSTATSQESVESMISHSTSDPAMTAKGIINLAKQSEQEPSSVTSTSLQLTEETVENRKVLSDWTTKKTTKAKDRTLRVCTYSASSSDAEPEPEYGTNYFQTAEEMLVELTKSNKQAEQEKQKQRKSVLGNPMSILDLYQHSLYGHFGEDGPEQLTHFSLIEQLSEPSGKDSKDKESPSNLKQTEETKEDPQTRLSKISLESLNKFNSNNVLLLEKEKNCLNKAEGQKEESGKKEASSLNSSDRQDMDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_015289927.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NCK-associated protein 5 (NCKAP5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289927.1

ORF Insert Sequence:

ATGGCCACAC AACTCTTTGA CCTCGGGCTG ACGAGGCGGG CTCTCCAGAA GCAATCTTCC 
CAGCACGGCA GGATTTACGA GGCACGCCAG CCGGCCAGAC CCAACGGTGC GCTGCCCCCT 
CAGCAGGCCG CGTCTCCTTC CCACGCTTCC GACGGCCCCG AGGCGGCACC TCCGCTCGGG 
GAGCGCCGCG GGCCGTACTG TGCGGCGCTG CCCCCGGGGG ATTTCGGCGG CGGGAGAGCC 
TCGCCAGGGG CAGCCCCGGC CCGGGACGAG CCGCCGGCCC CGGCCCCGCC GCCGCAGACA 
ATGGCCGCCC CCGCCCGACC CCCCCCCCAA CCCCCTCCCC GCCGCGGCGA GCCCCTGCTG 
CCGCCGCACG GTTGCCGGAG CCATTCAGTC GCGGCCGATC GCGACCGCGG CAGATGGGCA 
GGATCTGACG GCCCGGTGCT CCGCGGCTGC CCGGGGCCGG GGCGGGCGGC TCGGGCGGAG 
CCCCCCCCCC TCGCCCCCGC GCGAAGTGCC GCCCCCGCAT GCCGAGCGTC CCGCGGCCGC 
GGAGGGAGCG GGGCACTCCG CAGCGCGACG AAGCGGAGGA GGCGCGCGGC GGCCGGCGGC 
GGGGGGCAGC CCGGGGGGCG CGGCCCCGCA CCGCTCGTCG AGGCGGCGGC CGGCGCCGCG 
CTCCCCGGGC CCGGCGCGGG CCGCCCCCGG CCGCCGCAGC ATGCCCCGCC GGGCAGCAGG 
GAGAAGCTGT CTGTGGCTCG GCTGCAGCGA GAAGTTGCGC AGAGTAAGAG CGAAGGAACC 
ATGCGCGAGA AACTGATACA TGAGTTGGAG GAAGAAAGAC ATTTAAGACT TGAGAGTGAA 
AAACGATTAC GGGAGGTAAC ACTGGAGTCA GAGCGTAACA GAGCTCAGAT GCGTGGCCTA 
CAAGAGCAGT TTTCCAGAAT GGAGGAAACG GTGAGAAATC TACTACAGAG CCAAGGATCC 
CCAGGCCAGA ATGGAGAAGG AACTGTCAAT ATCATGAAGG CATATCAGGA AAAGCTGTCA 
GAAGACAGTA GGAAATGCAA AGAAGTCATG GAAAAAAATC ACACAGCAGT AGATGAAGAT 
TCAAGAAATG AAAGCAACAG CACTGAAGAG GAAAAAGAAA AGACAAAATT GCTACTGGAG 
CGTTTGAAAG CTCTGGAGGC AGAGAATTCA GCACTGGCTC TGGAAAATGA AAATCAGAGG 
GAACAGTATG AAAGATGTCT TGATGAGGTA GCTAATCAGG TCGTCCAGGC TCTACTTACT 
CAAAAGGATC TGAGGGAGGA ATGTATAAAG CTGAAAACGA GAGTTTTTGA TTTGGAGCAA 
CAGAATCAAA CATTAAGTGT GCTTTTCCAG CAGAGAGTCA AACCAGCTTC TGACCTCCTC 
CTTCAGAAAC TCCATTCGCG CATCTTAGAC CTCTCATCTG GAGATTTGCT GTCAGAAGTA 
GATCGAAACA GAAGCTCGGT GCCGTCACGA CCTGCTGATG CTCAGATTCA CGAATGCCAG 
CAGAATGTGA AATCCACTAT ACCTGTCTTG AAATGCCACA GTCAATTAAA TGTGACAGGT 
CCTAGCCGCC TGTATCCCCG GAGCAGCTGC AGTAGCAGTG AACTCTCCCT TTCAAGTGCT 
TGCAGTGAAT ATTCCAGTGG TTCCTCCTAC ACTTGGAATG ATGGGAAGAC GTGCAGCAAA 
AGGTCATCCG TGAGCTGGGA TAAAAGAATA AGCATTGGTT CTTCACTCCC AAGCAACCTC 
TCTAGTCCTG CAGATGATCT GCCTCCAACT CGTATCAAGG AAAACCATAT CCTAGAAGGA 
CTGAAGAAAT TACAGAGGCA AAAAGTATTA CTTGAATCAC CTTCAGTAAT ACCGAAATGG 
GGTTACAAAG ATTGCATGAA CTCCAATGAA GGAATATATT CCCCTGGAGT TAAATGTGGC 
AGCCATCATG AGCAGACTCA TTGTAAGCCA ATGGAAATAG GAAGTAGTTG CATTGAACAT 
AACAAAACTT TCTCTTATGA TTCAGACTCA CATGATGATG CAGATGATGA TTCTTCATCT 
TTATTATTGC TGCAGGAAGT ACCAAGCAAA GACTGCAGGA TCTATTGTAA CAAATTAACT 
CACAGTGTTT CTGACAGTCT CTTTCACTGG GATCCTAATA GGAAACATTT GCCAGAAAGA 
AGTTCATATT TTAATTCAAA GGAAAGACCA GAAAAATTGA CCAGTTTTGT CAGTGGATTT 
CAGGCAGAAG TAGAATCGTG CACCAGAACA AAGCTGCCTG ATTTACAGTT AGATCAAAGC 
CATGCTAATA TAGGCTGGAA GGACCTTAAT TTTCAGCTTT CAGATACTGA TGACAATGAA 
GTCTTGGATG AATTGCATAT AGAAAGCAGT GATGAGAAAA GTCCATCAGA TTTATTAGCA 
ACTCCTTTTA CTGAGAAGTA CACAAAGAAC TCTGATGTGC TCATAGTCAC AGAGAATTCT 
CAGTTTATAT CTAGTGACAA AGAGGAAAGA CAGACATCTG CTCACTCAGA CATTAGGCCA 
AAGACATGTA ATTTCATTAA ACAACAAAAA GTCATAAAAA AGACTTCTTC TGAGGAGTGC 
ATTACAGTGA TATTTGATGC TGAGGACGGT GAGCCTATTG AATTCAGCTC GCATCAAATT 
GGAGTTGTCA CTGTAACAAG AAATGAGATA TCAGTCAACC AACCAGAAAG TAGGTCCAGT 
GCAGAATATA CTGAACTTAT ACCTCAGGGA ATAGCTAATT TGCAGGCAGG AACAAATGCT 
AGAGACTACA GTGTTTTGGA AGGACCTGAA GACAAAACTA AAAAACAGAC TCTTGAAGAT 
AATACAGGGA ATAAAAATAT GGTTGTCACC ACAACCTGCA GTGAATCTTC ACCCTGTGGA 
AGAATCATAC TGCAAAATGC ACCACGACAA AAACTAGTGA AGCCAGTGTA TGATGTGTCG 
TACAAGACCA ATTCAAGCTC CATTGTTCAT GCAGGAGTCA ATCAAAAGCC AATCTTGACT 
AAAATACCCA GCAGAGGTAA ATTTTCTCCA CAAAAAAACA AGATGACAGA GAGTGAGGAG 
GCACCTGTAG CTACTTCAGT GACCCCTGCA ACCCTTGAAA AATCACCTGC TCTTCCACCT 
GTAAAGCTTT CAAGACTCAA AAAGGCACAA AGTCCACCTC GTAATTTTGA CTCAAAAGTT 
GACTTCCAAG TTCCAAAGCC CCCTGCCCAC CCCCTGCCCA GCTCAAAGCG TCCGAGCAGA 
GGCAATGGGA CAAAGTATCC AAAGAGTCAG AGTCCAGCAT CACCACTGTT GCCTCAGCAC 
CTTATAGAGC CTACTGACTA TGGAGAACCT CCAACTAGAG ACTCTAATCA TGATTTAGCA 
ACTGTAGAAG CCAGGTCGCC ATCCCCACCC CTTCCTCCCG GCAGGTCAAC GTCCCTATTG 
ATTCGTCCTG ATTATGCTCA TTCACAACCT GCGGCTGTGA AGTTAGGAGG AGCTGTTCCA 
GGTGAAACAG CAAAAAATAT ACTGAAACCA TCACCATTAA AAGGAACTGC AAACTCTGGC 
ATCCATAATC AATCTAGTCA TGATATGCAA GCAAAAAGTC TATCTTCTGG GGCCAAAATT 
GAGTTATCTT ACCTTCAAGA AAATTCTGAA GCAATTATGC AAAATAAATG TATATCTCAG 
CAACCCCATA GTGCATGCCA AGGACTTTCC AAAGTTTCCA CAAAGCAAGT CTGCCTGAAA 
AACACCAATC AGATGGGTCT CCACAGGAAC CGTGAGTTTC TGAGCATGGA TTCTCAGACA 
GGCAGGTCCA TTCCTCTGGG TCCGTCACTG GAAACAACTT CACAAGAAGT GCATTCCAGC 
AAGAAGGATA TTTCCAGTGA CAGTGGGGTT GATCAGCCAC AAAAGATGCC AAACATTCTC 
CCTGCAACTC CTCAATGTCA TACAGCAAGC GTGAGTGAGC CCTTGCTATC TCAGGTAAAC 
AATTCTCCAT TGTCAACACT GTTTCATGGT AGTGACACTG CAAATGCCAC ACAAAACTCT 
AATGAGAAAG GAGTGAAAAC CCGTCTCCCT GTAGGACTGA AATTATTTGT CAAATCTCCT 
CAACTTCTCC GAAAGAGCTC CACCGTCCCA GGGAAGCAGG AAAAAGATAG TATAAATGCA 
GCTTCCAAAA GCCACGTGAG TACAAGTAAG CACAAGCAAA ATGAACGTGT GGCCACCAAA 
GGTGCAATAG ATGCTGAATT AAAGGACTCT AAGTCTGATA CCGAAATAAA AGATAATTTT 
TCTTTGAGAC TTAGTGTGGA TACAGCATCA CCTCGTTCAC ATGAAAATGG CAGCTTGGTG 
GTAGACAGAG TAGATGAATT AGAAAGCAAA CTAGTTAAGA GATCTTTTTC TTCTAGCAAC 
AAGTCACACT TGAAACCAGC TCTAGGCATG AATGGAGCGA AAGCTCGAAG TCAGAGTTTC 
AGCGTTCATA CAGGAGAAAA GTCATCTACC CTTGTGACAG AAGGTCTTGG AAAAGTACGG 
ACTCAGATTA TTACAAACAC TTCTGACAGA GGAAATTCTC TAACAAGGCA GAACTCAGCC 
ATGGAAGGAC TTCAAATCAA GGCCATTCCT GGGTCAGGAG CAACACAAGA GACTCTTTCA 
AATACTTCAA AAAACACAGA AAATGCTTAC TCTAGACAAG GTTCTCTTGG AAGTAATAGC 
AATAACCAGC ATGGAAGCCC AAGCAAACTG CCATTCAGAT CATCTCCAAA AGTAGACTTA 
TTTCGAAACA CTTCAAAATA TGATGGAAAC AAATCATTTT CTATGAAAGA TGCACGGAGT 
CTATCTGTCC AGAGTGAGAA AAGCAGTGAA AATTTAAAAC ACGAAGCTCT AAATAAAAAG 
AGTGTTTTGC CAGCAGAATT GGAGTTAGAT ACATCAGCTA CTGTATCTTC CAAACAGATT 
TCATCAATTC ATAGCTTGGG TGGAATAAAA TGTCCGCATA AACTGGAAGC AATAAAATCA 
CAGAGACAGC AGATGTCTTT AAAGTTAGCA GAAGCCCCTC AGGTTACTGA TGTTTTTAGT 
TCACCTGCTC TGCAACCTAC AATAGAGGAG AAGGTCATGT TGTGCATCCA AGAAAATGTC 
CAGAAGGGTC AGGAACAAAA CAAATCTCCC ACTGTAGAGA CTAAACAAAA GACTGGGCCC 
TCTATAGCTA GTTGGTTTGG CTTTCGAAAG AGTAAGCTGC CAGCACTGAG CAGCAGGAAA 
CCTGACGTTC CTAAAACAAA GGTAGAAAAA AAAGAACCAA GAGTTTCAGG ATTTGGAATT 
AAGCAGTCGA AATTAGAGAG AAGAAAAGAA AAAAAGCAAA CTGAACAACA CTGTGAAGTA 
GAAAATGAAA TTAACAGGAA AACGGACAGC AGTGACTTTT TAGATGATGC TATGGAGTTT 
AAAATTTTGA AGCCATCACG AAACACGTCT GGCAAGATTG GATGTGAACA ACTCAGAGGT 
TCCTCCATAG CTACATATTC ATCAAAAGAC AGTTTTATGA AGGAACTTCT AAACAGAGTT 
GATAAGAAAG CAGCTCAGCA GACAGAAAGT GGATCAAATA ATGTTTCCTA CAGGAGTGTG 
TCGAAGGGCA GCTCCCAGGG CTCCTCTCTT CACAGCAACT CTATCAGCTC TCAAGGAAAC 
CACAAGAAAA ACAGCAACAC AAAAGCTGAT ATGGAAATAC AGAATCAGAC CCTGGCTAAC 
GTAGTCGCAG AAAACCTGCA AGAAGAAGAG GAGGACACCA TGACAAGGAC TACATGTCAG 
AGTCATGTGA TAGAGTCTTG CTGCCAGATG AGGACGTTGG ACAGCGGGAT TGGAACCTTC 
CCACTCCCCG ACTCAGGGAA CCGATCTGCG GGGCGACACG TGTCTAAGCA GGAATTAACC 
TTGGAAACAG AAGCCCTAGC ACCGTCTGAG CAAGTTCTTC TTTCAGCTCC TTCAGTAAAA 
GCCAAAACTC TTGAGCGAGA AGTGCCTTCA ACAGCTACGA GTCAGGAGTC TGTGGAAAGC 
ATGATTAGTC ACTCAACATC TGATCCAGCC ATGACTGCCA AAGGAATAAT TAACCTTGCA 
AAGCAAAGTG AACAAGAACC AAGTTCTGTG ACCTCTACCT CATTACAGCT TACTGAAGAA 
ACTGTAGAAA ATAGAAAAGT TCTTTCAGAT TGGACCACCA AGAAAACTAC AAAAGCAAAG 
GACAGAACTC TAAGAGTGTG CACTTACTCT GCCAGCAGCA GTGACGCTGA ACCGGAGCCA 
GAGTATGGAA CAAATTATTT TCAAACTGCA GAGGAGATGT TAGTAGAGTT AACAAAGAGC 
AACAAACAAG CTGAGCAGGA AAAACAGAAG CAGAGAAAGT CAGTTTTGGG GAACCCGATG 
TCAATCTTGG ATTTATACCA GCACAGTCTC TATGGCCATT TTGGAGAGGA TGGACCTGAA 
CAATTAACAC ATTTCAGTTT AATTGAGCAG CTGAGTGAAC CTTCCGGTAA AGACAGCAAG 
GACAAAGAGA GCCCAAGTAA CCTGAAGCAA ACTGAAGAAA CAAAAGAAGA TCCTCAGACT 
AGATTATCTA AAATTTCCCT GGAGTCTCTC AATAAATTTA ACAGCAATAA CGTCCTTTTA 
TTAGAAAAGG AGAAGAACTG TCTGAACAAA GCTGAAGGAC AAAAGGAAGA AAGCGGAAAG 
AAGGAAGCAT CTTCCTTAAA CAGCTCCGAT AGGCAGGACA TGGACAATTT GGAGTCCCTG 
AGCGATTCTT TGTACGACAG CTTCTCCTCT TGTGCCAGCC AAGGCTCTAA CGACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_015289928.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145414.1 Gallus gallus NCK-associated protein 5 (NCKAP5), transcript variant X3, mRNA.

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CloneID	OGa38637
Clone ID Related Accession (Same CDS sequence)	XM_015289928.1
Accession Version	XM_015289928.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	nck-associated protein 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAC AACTCTTTGA CCTCGGGCTG ACGAGGCGGG CTCTCCAGAA GCAATCTTCC 
CAGCACGGCA GGATTTACGA GGCACGCCAG CCGGCCAGAC CCAACGGTGC GCTGCCCCCT 
CAGCAGGCCG CGTCTCCTTC CCACGCTTCC GACGGCCCCG AGGCGGCACC TCCGCTCGGG 
GAGCGCCGCG GGCCGTACTG TGCGGCGCTG CCCCCGGGGG ATTTCGGCGG CGGGAGAGCC 
TCGCCAGGGG CAGCCCCGGC CCGGGACGAG CCGCCGGCCC CGGCCCCGCC GCCGCAGACA 
ATGGCCGCCC CCGCCCGACC CCCCCCCCAA CCCCCTCCCC GCCGCGGCGA GCCCCTGCTG 
CCGCCGCACG GTTGCCGGAG CCATTCAGTC GCGGCCGATC GCGACCGCGG CAGATGGGCA 
GGATCTGACG GCCCGGTGCT CCGCGGCTGC CCGGGGCCGG GGCGGGCGGC TCGGGCGGAG 
CCCCCCCCCC TCGCCCCCGC GCGAAGTGCC GCCCCCGCAT GCCGAGCGTC CCGCGGCCGC 
GGAGGGAGCG GGGCACTCCG CAGCGCGACG AAGCGGAGGA GGCGCGCGGC GGCCGGCGGC 
GGGGGGCAGC CCGGGGGGCG CGGCCCCGCA CCGCTCGTCG AGGCGGCGGC CGGCGCCGCG 
CTCCCCGGGC CCGGCGCGGG CCGCCCCCGG CCGCCGCAGC ATGCCCCGCC GGGCAGCAGG 
GAGAAGCTGT CTGTGGCTCG GCTGCAGCGA GAAGTTGCGC AGAGTAAGAG CGAAGGAACC 
ATGCGCGAGA AACTGATACA TGAGTTGGAG GAAGAAAGAC ATTTAAGACT TGAGAGTGAA 
AAACGATTAC GGGAGGTAAC ACTGGAGTCA GAGCGTAACA GAGCTCAGAT GCGTGGCCTA 
CAAGAGCAGT TTTCCAGAAT GGAGGAAACG GTGAGAAATC TACTACAGAG CCAAGGATCC 
CCAGGCCAGA ATGGAGAAGG AACTGTCAAT ATCATGAAGG CATATCAGGA AAAGCTGTCA 
GAAGACAGTA GGAAATGCAA AGAAGTCATG GAAAAAAATC ACACAGCAGT AGATGAAGAT 
TCAAGAAATG AAAGCAACAG CACTGAAGAG GAAAAAGAAA AGACAAAATT GCTACTGGAG 
CGTTTGAAAG CTCTGGAGGC AGAGAATTCA GCACTGGCTC TGGAAAATGA AAATCAGAGG 
GAACAGTATG AAAGATGTCT TGATGAGGTA GCTAATCAGG TCGTCCAGGC TCTACTTACT 
CAAAAGGATC TGAGGGAGGA ATGTATAAAG CTGAAAACGA GAGTTTTTGA TTTGGAGCAA 
CAGAATCAAA CATTAAGTGT GCTTTTCCAG CAGAGAGTCA AACCAGCTTC TGACCTCCTC 
CTTCAGAAAC TCCATTCGCG CATCTTAGAC CTCTCATCTG GAGATTTGCT GTCAGAAGTA 
GATCGAAACA GAAGCTCGGT GCCGTCACGA CCTGCTGATG CTCAGATTCA CGAATGCCAG 
CAGAATGTGA AATCCACTAT ACCTGTCTTG AAATGCCACA GTCAATTAAA TGTGACAGGT 
CCTAGCCGCC TGTATCCCCG GAGCAGCTGC AGTAGCAGTG AACTCTCCCT TTCAAGTGCT 
TGCAGTGAAT ATTCCAGTGG TTCCTCCTAC ACTTGGAATG ATGGGAAGAC GTGCAGCAAA 
AGGTCATCCG TGAGCTGGGA TAAAAGAATA AGCATTGGTT CTTCACTCCC AAGCAACCTC 
TCTAGTCCTG CAGATGATCT GCCTCCAACT CGTATCAAGG AAAACCATAT CCTAGAAGGA 
CTGAAGAAAT TACAGAGGCA AAAAGTATTA CTTGAATCAC CTTCAGTAAT ACCGAAATGG 
GGTTACAAAG ATTGCATGAA CTCCAATGAA GGAATATATT CCCCTGGAGT TAAATGTGGC 
AGCCATCATG AGCAGACTCA TTGTAAGCCA ATGGAAATAG GAAGTAGTTG CATTGAACAT 
AACAAAACTT TCTCTTATGA TTCAGACTCA CATGATGATG CAGATGATGA TTCTTCATCT 
TTATTATTGC TGCAGGAAGT ACCAAGCAAA GACTGCAGGA TCTATTGTAA CAAATTAACT 
CACAGTGTTT CTGACAGTCT CTTTCACTGG GATCCTAATA GGAAACATTT GCCAGAAAGA 
AGTTCATATT TTAATTCAAA GGAAAGACCA GAAAAATTGA CCAGTTTTGT CAGTGGATTT 
CAGGCAGAAG TAGAATCGTG CACCAGAACA AAGCTGCCTG ATTTACAGTT AGATCAAAGC 
CATGCTAATA TAGGCTGGAA GGACCTTAAT TTTCAGCTTT CAGATACTGA TGACAATGAA 
GTCTTGGATG AATTGCATAT AGAAAGCAGT GATGAGAAAA GTCCATCAGA TTTATTAGCA 
ACTCCTTTTA CTGAGAAGTA CACAAAGAAC TCTGATGTGC TCATAGTCAC AGAGAATTCT 
CAGTTTATAT CTAGTGACAA AGAGGAAAGA CAGACATCTG CTCACTCAGA CATTAGGCCA 
AAGACATGTA ATTTCATTAA ACAACAAAAA GTCATAAAAA AGACTTCTTC TGAGGAGTGC 
ATTACAGTGA TATTTGATGC TGAGGACGGT GAGCCTATTG AATTCAGCTC GCATCAAATT 
GGAGTTGTCA CTGTAACAAG AAATGAGATA TCAGTCAACC AACCAGAAAG TAGGTCCAGT 
GCAGAATATA CTGAACTTAT ACCTCAGGGA ATAGCTAATT TGCAGGCAGG AACAAATGCT 
AGAGACTACA GTGTTTTGGA AGGACCTGAA GACAAAACTA AAAAACAGAC TCTTGAAGAT 
AATACAGGGA ATAAAAATAT GGTTGTCACC ACAACCTGCA GTGAATCTTC ACCCTGTGGA 
AGAATCATAC TGCAAAATGC ACCACGACAA AAACTAGTGA AGCCAGTGTA TGATGTGTCG 
TACAAGACCA ATTCAAGCTC CATTGTTCAT GCAGGAGTCA ATCAAAAGCC AATCTTGACT 
AAAATACCCA GCAGAGGTAA ATTTTCTCCA CAAAAAAACA AGATGACAGA GAGTGAGGAG 
GCACCTGTAG CTACTTCAGT GACCCCTGCA ACCCTTGAAA AATCACCTGC TCTTCCACCT 
GTAAAGCTTT CAAGACTCAA AAAGGCACAA AGTCCACCTC GTAATTTTGA CTCAAAAGTT 
GACTTCCAAG TTCCAAAGCC CCCTGCCCAC CCCCTGCCCA GCTCAAAGCG TCCGAGCAGA 
GGCAATGGGA CAAAGTATCC AAAGAGTCAG AGTCCAGCAT CACCACTGTT GCCTCAGCAC 
CTTATAGAGC CTACTGACTA TGGAGAACCT CCAACTAGAG ACTCTAATCA TGATTTAGCA 
ACTGTAGAAG CCAGGTCGCC ATCCCCACCC CTTCCTCCCG GCAGGTCAAC GTCCCTATTG 
ATTCGTCCTG ATTATGCTCA TTCACAACCT GCGGCTGTGA AGTTAGGAGG AGCTGTTCCA 
GGTGAAACAG CAAAAAATAT ACTGAAACCA TCACCATTAA AAGGAACTGC AAACTCTGGC 
ATCCATAATC AATCTAGTCA TGATATGCAA GCAAAAAGTC TATCTTCTGG GGCCAAAATT 
GAGTTATCTT ACCTTCAAGA AAATTCTGAA GCAATTATGC AAAATAAATG TATATCTCAG 
CAACCCCATA GTGCATGCCA AGGACTTTCC AAAGTTTCCA CAAAGCAAGT CTGCCTGAAA 
AACACCAATC AGATGGGTCT CCACAGGAAC CGTGAGTTTC TGAGCATGGA TTCTCAGACA 
GGCAGGTCCA TTCCTCTGGG TCCGTCACTG GAAACAACTT CACAAGAAGT GCATTCCAGC 
AAGAAGGATA TTTCCAGTGA CAGTGGGGTT GATCAGCCAC AAAAGATGCC AAACATTCTC 
CCTGCAACTC CTCAATGTCA TACAGCAAGC GTGAGTGAGC CCTTGCTATC TCAGGTAAAC 
AATTCTCCAT TGTCAACACT GTTTCATGGT AGTGACACTG CAAATGCCAC ACAAAACTCT 
AATGAGAAAG GAGTGAAAAC CCGTCTCCCT GTAGGACTGA AATTATTTGT CAAATCTCCT 
CAACTTCTCC GAAAGAGCTC CACCGTCCCA GGGAAGCAGG AAAAAGATAG TATAAATGCA 
GCTTCCAAAA GCCACGTGAG TACAAGTAAG CACAAGCAAA ATGAACGTGT GGCCACCAAA 
GGTGCAATAG ATGCTGAATT AAAGGACTCT AAGTCTGATA CCGAAATAAA AGATAATTTT 
TCTTTGAGAC TTAGTGTGGA TACAGCATCA CCTCGTTCAC ATGAAAATGG CAGCTTGGTG 
GTAGACAGAG TAGATGAATT AGAAAGCAAA CTAGTTAAGA GATCTTTTTC TTCTAGCAAC 
AAGTCACACT TGAAACCAGC TCTAGGCATG AATGGAGCGA AAGCTCGAAG TCAGAGTTTC 
AGCGTTCATA CAGGAGAAAA GTCATCTACC CTTGTGACAG AAGGTCTTGG AAAAGTACGG 
ACTCAGATTA TTACAAACAC TTCTGACAGA GGAAATTCTC TAACAAGGCA GAACTCAGCC 
ATGGAAGGAC TTCAAATCAA GGCCATTCCT GGGTCAGGAG CAACACAAGA GACTCTTTCA 
AATACTTCAA AAAACACAGA AAATGCTTAC TCTAGACAAG GTTCTCTTGG AAGTAATAGC 
AATAACCAGC ATGGAAGCCC AAGCAAACTG CCATTCAGAT CATCTCCAAA AGTAGACTTA 
TTTCGAAACA CTTCAAAATA TGATGGAAAC AAATCATTTT CTATGAAAGA TGCACGGAGT 
CTATCTGTCC AGAGTGAGAA AAGCAGTGAA AATTTAAAAC ACGAAGCTCT AAATAAAAAG 
AGTGTTTTGC CAGCAGAATT GGAGTTAGAT ACATCAGCTA CTGTATCTTC CAAACAGATT 
TCATCAATTC ATAGCTTGGG TGGAATAAAA TGTCCGCATA AACTGGAAGC AATAAAATCA 
CAGAGACAGC AGATGTCTTT AAAGTTAGCA GAAGCCCCTC AGGTTACTGA TGTTTTTAGT 
TCACCTGCTC TGCAACCTAC AATAGAGGAG AAGGTCATGT TGTGCATCCA AGAAAATGTC 
CAGAAGGGTC AGGAACAAAA CAAATCTCCC ACTGTAGAGA CTAAACAAAA GACTGGGCCC 
TCTATAGCTA GTTGGTTTGG CTTTCGAAAG AGTAAGCTGC CAGCACTGAG CAGCAGGAAA 
CCTGACGTTC CTAAAACAAA GGTAGAAAAA AAAGAACCAA GAGTTTCAGG ATTTGGAATT 
AAGCAGTCGA AATTAGAGAG AAGAAAAGAA AAAAAGCAAA CTGAACAACA CTGTGAAGTA 
GAAAATGAAA TTAACAGGAA AACGGACAGC AGTGACTTTT TAGATGATGC TATGGAGTTT 
AAAATTTTGA AGCCATCACG AAACACGTCT GGCAAGATTG GATGTGAACA ACTCAGAGGT 
TCCTCCATAG CTACATATTC ATCAAAAGAC AGTTTTATGA AGGAACTTCT AAACAGGAGT 
GTGTCGAAGG GCAGCTCCCA GGGCTCCTCT CTTCACAGCA ACTCTATCAG CTCTCAAGGA 
AACCACAAGA AAAACAGCAA CACAAAAGCT GATATGGAAA TACAGAATCA GACCCTGGCT 
AACGTAGTCG CAGAAAACCT GCAAGAAGAA GAGGAGGACA CCATGACAAG GACTACATGT 
CAGAGTCATG TGATAGAGTC TTGCTGCCAG ATGAGGACGT TGGACAGCGG GATTGGAACC 
TTCCCACTCC CCGACTCAGG GAACCGATCT GCGGGGCGAC ACGTGTCTAA GCAGGAATTA 
ACCTTGGAAA CAGAAGCCCT AGCACCGTCT GAGCAAGTTC TTCTTTCAGC TCCTTCAGTA 
AAAGCCAAAA CTCTTGAGCG AGAAGTGCCT TCAACAGCTA CGAGTCAGGA GTCTGTGGAA 
AGCATGATTA GTCACTCAAC ATCTGATCCA GCCATGACTG CCAAAGGTAT ACGACCTTTT 
CAAAGTCGCC TTCCAAAACC AGATTCCTCA GGAATAATTA ACCTTGCAAA GCAAAGTGAA 
CAAGAACCAA GTTCTGTGAC CTCTACCTCA TTACAGCTTA CTGAAGAAAC TGTAGAAAAT 
AGAAAAGTTC TTTCAGATTG GACCACCAAG AAAACTACAA AAGCAAAGGA CAGAACTCTA 
AGAGTGTGCA CTTACTCTGC CAGCAGCAGT GACGCTGAAC CGGAGCCAGA GTATGGAACA 
AATTATTTTC AAACTGCAGA GGAGATGTTA GTAGAGTTAA CAAAGAGCAA CAAACAAGCT 
GAGCAGGAAA AACAGAAGCA GAGAAAGTCA GTTTTGGGGA ACCCGATGTC AATCTTGGAT 
TTATACCAGC ACAGTCTCTA TGGCCATTTT GGAGAGGATG GACCTGAACA ATTAACACAT 
TTCAGTTTAA TTGAGCAGCT GAGTGAACCT TCCGGTAAAG ACAGCAAGGA CAAAGAGAGC 
CCAAGTAACC TGAAGCAAAC TGAAGAAACA AAAGAAGATC CTCAGACTAG ATTATCTAAA 
ATTTCCCTGG AGTCTCTCAA TAAATTTAAC AGCAATAACG TCCTTTTATT AGAAAAGGAG 
AAGAACTGTC TGAACAAAGC TGAAGGACAA AAGGAAGAAA GCGGAAAGAA GGAAGCATCT 
TCCTTAAACA GCTCCGATAG GCAGGACATG GACAATTTGG AGTCCCTGAG CGATTCTTTG 
TACGACAGCT TCTCCTCTTG TGCCAGCCAA GGCTCTAACG ACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145414.1
CDS	5357..12124
Translation	MATQLFDLGLTRRALQKQSSQHGRIYEARQPARPNGALPPQQAASPSHASDGPEAAPPLGERRGPYCAALPPGDFGGGRASPGAAPARDEPPAPAPPPQTMAAPARPPPQPPPRRGEPLLPPHGCRSHSVAADRDRGRWAGSDGPVLRGCPGPGRAARAEPPPLAPARSAAPACRASRGRGGSGALRSATKRRRRAAAGGGGQPGGRGPAPLVEAAAGAALPGPGAGRPRPPQHAPPGSREKLSVARLQREVAQSKSEGTMREKLIHELEEERHLRLESEKRLREVTLESERNRAQMRGLQEQFSRMEETVRNLLQSQGSPGQNGEGTVNIMKAYQEKLSEDSRKCKEVMEKNHTAVDEDSRNESNSTEEEKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECIKLKTRVFDLEQQNQTLSVLFQQRVKPASDLLLQKLHSRILDLSSGDLLSEVDRNRSSVPSRPADAQIHECQQNVKSTIPVLKCHSQLNVTGPSRLYPRSSCSSSELSLSSACSEYSSGSSYTWNDGKTCSKRSSVSWDKRISIGSSLPSNLSSPADDLPPTRIKENHILEGLKKLQRQKVLLESPSVIPKWGYKDCMNSNEGIYSPGVKCGSHHEQTHCKPMEIGSSCIEHNKTFSYDSDSHDDADDDSSSLLLLQEVPSKDCRIYCNKLTHSVSDSLFHWDPNRKHLPERSSYFNSKERPEKLTSFVSGFQAEVESCTRTKLPDLQLDQSHANIGWKDLNFQLSDTDDNEVLDELHIESSDEKSPSDLLATPFTEKYTKNSDVLIVTENSQFISSDKEERQTSAHSDIRPKTCNFIKQQKVIKKTSSEECITVIFDAEDGEPIEFSSHQIGVVTVTRNEISVNQPESRSSAEYTELIPQGIANLQAGTNARDYSVLEGPEDKTKKQTLEDNTGNKNMVVTTTCSESSPCGRIILQNAPRQKLVKPVYDVSYKTNSSSIVHAGVNQKPILTKIPSRGKFSPQKNKMTESEEAPVATSVTPATLEKSPALPPVKLSRLKKAQSPPRNFDSKVDFQVPKPPAHPLPSSKRPSRGNGTKYPKSQSPASPLLPQHLIEPTDYGEPPTRDSNHDLATVEARSPSPPLPPGRSTSLLIRPDYAHSQPAAVKLGGAVPGETAKNILKPSPLKGTANSGIHNQSSHDMQAKSLSSGAKIELSYLQENSEAIMQNKCISQQPHSACQGLSKVSTKQVCLKNTNQMGLHRNREFLSMDSQTGRSIPLGPSLETTSQEVHSSKKDISSDSGVDQPQKMPNILPATPQCHTASVSEPLLSQVNNSPLSTLFHGSDTANATQNSNEKGVKTRLPVGLKLFVKSPQLLRKSSTVPGKQEKDSINAASKSHVSTSKHKQNERVATKGAIDAELKDSKSDTEIKDNFSLRLSVDTASPRSHENGSLVVDRVDELESKLVKRSFSSSNKSHLKPALGMNGAKARSQSFSVHTGEKSSTLVTEGLGKVRTQIITNTSDRGNSLTRQNSAMEGLQIKAIPGSGATQETLSNTSKNTENAYSRQGSLGSNSNNQHGSPSKLPFRSSPKVDLFRNTSKYDGNKSFSMKDARSLSVQSEKSSENLKHEALNKKSVLPAELELDTSATVSSKQISSIHSLGGIKCPHKLEAIKSQRQQMSLKLAEAPQVTDVFSSPALQPTIEEKVMLCIQENVQKGQEQNKSPTVETKQKTGPSIASWFGFRKSKLPALSSRKPDVPKTKVEKKEPRVSGFGIKQSKLERRKEKKQTEQHCEVENEINRKTDSSDFLDDAMEFKILKPSRNTSGKIGCEQLRGSSIATYSSKDSFMKELLNRSVSKGSSQGSSLHSNSISSQGNHKKNSNTKADMEIQNQTLANVVAENLQEEEEDTMTRTTCQSHVIESCCQMRTLDSGIGTFPLPDSGNRSAGRHVSKQELTLETEALAPSEQVLLSAPSVKAKTLEREVPSTATSQESVESMISHSTSDPAMTAKGIRPFQSRLPKPDSSGIINLAKQSEQEPSSVTSTSLQLTEETVENRKVLSDWTTKKTTKAKDRTLRVCTYSASSSDAEPEPEYGTNYFQTAEEMLVELTKSNKQAEQEKQKQRKSVLGNPMSILDLYQHSLYGHFGEDGPEQLTHFSLIEQLSEPSGKDSKDKESPSNLKQTEETKEDPQTRLSKISLESLNKFNSNNVLLLEKEKNCLNKAEGQKEESGKKEASSLNSSDRQDMDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_015289928.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NCK-associated protein 5 (NCKAP5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289928.1

ORF Insert Sequence:

ATGGCCACAC AACTCTTTGA CCTCGGGCTG ACGAGGCGGG CTCTCCAGAA GCAATCTTCC 
CAGCACGGCA GGATTTACGA GGCACGCCAG CCGGCCAGAC CCAACGGTGC GCTGCCCCCT 
CAGCAGGCCG CGTCTCCTTC CCACGCTTCC GACGGCCCCG AGGCGGCACC TCCGCTCGGG 
GAGCGCCGCG GGCCGTACTG TGCGGCGCTG CCCCCGGGGG ATTTCGGCGG CGGGAGAGCC 
TCGCCAGGGG CAGCCCCGGC CCGGGACGAG CCGCCGGCCC CGGCCCCGCC GCCGCAGACA 
ATGGCCGCCC CCGCCCGACC CCCCCCCCAA CCCCCTCCCC GCCGCGGCGA GCCCCTGCTG 
CCGCCGCACG GTTGCCGGAG CCATTCAGTC GCGGCCGATC GCGACCGCGG CAGATGGGCA 
GGATCTGACG GCCCGGTGCT CCGCGGCTGC CCGGGGCCGG GGCGGGCGGC TCGGGCGGAG 
CCCCCCCCCC TCGCCCCCGC GCGAAGTGCC GCCCCCGCAT GCCGAGCGTC CCGCGGCCGC 
GGAGGGAGCG GGGCACTCCG CAGCGCGACG AAGCGGAGGA GGCGCGCGGC GGCCGGCGGC 
GGGGGGCAGC CCGGGGGGCG CGGCCCCGCA CCGCTCGTCG AGGCGGCGGC CGGCGCCGCG 
CTCCCCGGGC CCGGCGCGGG CCGCCCCCGG CCGCCGCAGC ATGCCCCGCC GGGCAGCAGG 
GAGAAGCTGT CTGTGGCTCG GCTGCAGCGA GAAGTTGCGC AGAGTAAGAG CGAAGGAACC 
ATGCGCGAGA AACTGATACA TGAGTTGGAG GAAGAAAGAC ATTTAAGACT TGAGAGTGAA 
AAACGATTAC GGGAGGTAAC ACTGGAGTCA GAGCGTAACA GAGCTCAGAT GCGTGGCCTA 
CAAGAGCAGT TTTCCAGAAT GGAGGAAACG GTGAGAAATC TACTACAGAG CCAAGGATCC 
CCAGGCCAGA ATGGAGAAGG AACTGTCAAT ATCATGAAGG CATATCAGGA AAAGCTGTCA 
GAAGACAGTA GGAAATGCAA AGAAGTCATG GAAAAAAATC ACACAGCAGT AGATGAAGAT 
TCAAGAAATG AAAGCAACAG CACTGAAGAG GAAAAAGAAA AGACAAAATT GCTACTGGAG 
CGTTTGAAAG CTCTGGAGGC AGAGAATTCA GCACTGGCTC TGGAAAATGA AAATCAGAGG 
GAACAGTATG AAAGATGTCT TGATGAGGTA GCTAATCAGG TCGTCCAGGC TCTACTTACT 
CAAAAGGATC TGAGGGAGGA ATGTATAAAG CTGAAAACGA GAGTTTTTGA TTTGGAGCAA 
CAGAATCAAA CATTAAGTGT GCTTTTCCAG CAGAGAGTCA AACCAGCTTC TGACCTCCTC 
CTTCAGAAAC TCCATTCGCG CATCTTAGAC CTCTCATCTG GAGATTTGCT GTCAGAAGTA 
GATCGAAACA GAAGCTCGGT GCCGTCACGA CCTGCTGATG CTCAGATTCA CGAATGCCAG 
CAGAATGTGA AATCCACTAT ACCTGTCTTG AAATGCCACA GTCAATTAAA TGTGACAGGT 
CCTAGCCGCC TGTATCCCCG GAGCAGCTGC AGTAGCAGTG AACTCTCCCT TTCAAGTGCT 
TGCAGTGAAT ATTCCAGTGG TTCCTCCTAC ACTTGGAATG ATGGGAAGAC GTGCAGCAAA 
AGGTCATCCG TGAGCTGGGA TAAAAGAATA AGCATTGGTT CTTCACTCCC AAGCAACCTC 
TCTAGTCCTG CAGATGATCT GCCTCCAACT CGTATCAAGG AAAACCATAT CCTAGAAGGA 
CTGAAGAAAT TACAGAGGCA AAAAGTATTA CTTGAATCAC CTTCAGTAAT ACCGAAATGG 
GGTTACAAAG ATTGCATGAA CTCCAATGAA GGAATATATT CCCCTGGAGT TAAATGTGGC 
AGCCATCATG AGCAGACTCA TTGTAAGCCA ATGGAAATAG GAAGTAGTTG CATTGAACAT 
AACAAAACTT TCTCTTATGA TTCAGACTCA CATGATGATG CAGATGATGA TTCTTCATCT 
TTATTATTGC TGCAGGAAGT ACCAAGCAAA GACTGCAGGA TCTATTGTAA CAAATTAACT 
CACAGTGTTT CTGACAGTCT CTTTCACTGG GATCCTAATA GGAAACATTT GCCAGAAAGA 
AGTTCATATT TTAATTCAAA GGAAAGACCA GAAAAATTGA CCAGTTTTGT CAGTGGATTT 
CAGGCAGAAG TAGAATCGTG CACCAGAACA AAGCTGCCTG ATTTACAGTT AGATCAAAGC 
CATGCTAATA TAGGCTGGAA GGACCTTAAT TTTCAGCTTT CAGATACTGA TGACAATGAA 
GTCTTGGATG AATTGCATAT AGAAAGCAGT GATGAGAAAA GTCCATCAGA TTTATTAGCA 
ACTCCTTTTA CTGAGAAGTA CACAAAGAAC TCTGATGTGC TCATAGTCAC AGAGAATTCT 
CAGTTTATAT CTAGTGACAA AGAGGAAAGA CAGACATCTG CTCACTCAGA CATTAGGCCA 
AAGACATGTA ATTTCATTAA ACAACAAAAA GTCATAAAAA AGACTTCTTC TGAGGAGTGC 
ATTACAGTGA TATTTGATGC TGAGGACGGT GAGCCTATTG AATTCAGCTC GCATCAAATT 
GGAGTTGTCA CTGTAACAAG AAATGAGATA TCAGTCAACC AACCAGAAAG TAGGTCCAGT 
GCAGAATATA CTGAACTTAT ACCTCAGGGA ATAGCTAATT TGCAGGCAGG AACAAATGCT 
AGAGACTACA GTGTTTTGGA AGGACCTGAA GACAAAACTA AAAAACAGAC TCTTGAAGAT 
AATACAGGGA ATAAAAATAT GGTTGTCACC ACAACCTGCA GTGAATCTTC ACCCTGTGGA 
AGAATCATAC TGCAAAATGC ACCACGACAA AAACTAGTGA AGCCAGTGTA TGATGTGTCG 
TACAAGACCA ATTCAAGCTC CATTGTTCAT GCAGGAGTCA ATCAAAAGCC AATCTTGACT 
AAAATACCCA GCAGAGGTAA ATTTTCTCCA CAAAAAAACA AGATGACAGA GAGTGAGGAG 
GCACCTGTAG CTACTTCAGT GACCCCTGCA ACCCTTGAAA AATCACCTGC TCTTCCACCT 
GTAAAGCTTT CAAGACTCAA AAAGGCACAA AGTCCACCTC GTAATTTTGA CTCAAAAGTT 
GACTTCCAAG TTCCAAAGCC CCCTGCCCAC CCCCTGCCCA GCTCAAAGCG TCCGAGCAGA 
GGCAATGGGA CAAAGTATCC AAAGAGTCAG AGTCCAGCAT CACCACTGTT GCCTCAGCAC 
CTTATAGAGC CTACTGACTA TGGAGAACCT CCAACTAGAG ACTCTAATCA TGATTTAGCA 
ACTGTAGAAG CCAGGTCGCC ATCCCCACCC CTTCCTCCCG GCAGGTCAAC GTCCCTATTG 
ATTCGTCCTG ATTATGCTCA TTCACAACCT GCGGCTGTGA AGTTAGGAGG AGCTGTTCCA 
GGTGAAACAG CAAAAAATAT ACTGAAACCA TCACCATTAA AAGGAACTGC AAACTCTGGC 
ATCCATAATC AATCTAGTCA TGATATGCAA GCAAAAAGTC TATCTTCTGG GGCCAAAATT 
GAGTTATCTT ACCTTCAAGA AAATTCTGAA GCAATTATGC AAAATAAATG TATATCTCAG 
CAACCCCATA GTGCATGCCA AGGACTTTCC AAAGTTTCCA CAAAGCAAGT CTGCCTGAAA 
AACACCAATC AGATGGGTCT CCACAGGAAC CGTGAGTTTC TGAGCATGGA TTCTCAGACA 
GGCAGGTCCA TTCCTCTGGG TCCGTCACTG GAAACAACTT CACAAGAAGT GCATTCCAGC 
AAGAAGGATA TTTCCAGTGA CAGTGGGGTT GATCAGCCAC AAAAGATGCC AAACATTCTC 
CCTGCAACTC CTCAATGTCA TACAGCAAGC GTGAGTGAGC CCTTGCTATC TCAGGTAAAC 
AATTCTCCAT TGTCAACACT GTTTCATGGT AGTGACACTG CAAATGCCAC ACAAAACTCT 
AATGAGAAAG GAGTGAAAAC CCGTCTCCCT GTAGGACTGA AATTATTTGT CAAATCTCCT 
CAACTTCTCC GAAAGAGCTC CACCGTCCCA GGGAAGCAGG AAAAAGATAG TATAAATGCA 
GCTTCCAAAA GCCACGTGAG TACAAGTAAG CACAAGCAAA ATGAACGTGT GGCCACCAAA 
GGTGCAATAG ATGCTGAATT AAAGGACTCT AAGTCTGATA CCGAAATAAA AGATAATTTT 
TCTTTGAGAC TTAGTGTGGA TACAGCATCA CCTCGTTCAC ATGAAAATGG CAGCTTGGTG 
GTAGACAGAG TAGATGAATT AGAAAGCAAA CTAGTTAAGA GATCTTTTTC TTCTAGCAAC 
AAGTCACACT TGAAACCAGC TCTAGGCATG AATGGAGCGA AAGCTCGAAG TCAGAGTTTC 
AGCGTTCATA CAGGAGAAAA GTCATCTACC CTTGTGACAG AAGGTCTTGG AAAAGTACGG 
ACTCAGATTA TTACAAACAC TTCTGACAGA GGAAATTCTC TAACAAGGCA GAACTCAGCC 
ATGGAAGGAC TTCAAATCAA GGCCATTCCT GGGTCAGGAG CAACACAAGA GACTCTTTCA 
AATACTTCAA AAAACACAGA AAATGCTTAC TCTAGACAAG GTTCTCTTGG AAGTAATAGC 
AATAACCAGC ATGGAAGCCC AAGCAAACTG CCATTCAGAT CATCTCCAAA AGTAGACTTA 
TTTCGAAACA CTTCAAAATA TGATGGAAAC AAATCATTTT CTATGAAAGA TGCACGGAGT 
CTATCTGTCC AGAGTGAGAA AAGCAGTGAA AATTTAAAAC ACGAAGCTCT AAATAAAAAG 
AGTGTTTTGC CAGCAGAATT GGAGTTAGAT ACATCAGCTA CTGTATCTTC CAAACAGATT 
TCATCAATTC ATAGCTTGGG TGGAATAAAA TGTCCGCATA AACTGGAAGC AATAAAATCA 
CAGAGACAGC AGATGTCTTT AAAGTTAGCA GAAGCCCCTC AGGTTACTGA TGTTTTTAGT 
TCACCTGCTC TGCAACCTAC AATAGAGGAG AAGGTCATGT TGTGCATCCA AGAAAATGTC 
CAGAAGGGTC AGGAACAAAA CAAATCTCCC ACTGTAGAGA CTAAACAAAA GACTGGGCCC 
TCTATAGCTA GTTGGTTTGG CTTTCGAAAG AGTAAGCTGC CAGCACTGAG CAGCAGGAAA 
CCTGACGTTC CTAAAACAAA GGTAGAAAAA AAAGAACCAA GAGTTTCAGG ATTTGGAATT 
AAGCAGTCGA AATTAGAGAG AAGAAAAGAA AAAAAGCAAA CTGAACAACA CTGTGAAGTA 
GAAAATGAAA TTAACAGGAA AACGGACAGC AGTGACTTTT TAGATGATGC TATGGAGTTT 
AAAATTTTGA AGCCATCACG AAACACGTCT GGCAAGATTG GATGTGAACA ACTCAGAGGT 
TCCTCCATAG CTACATATTC ATCAAAAGAC AGTTTTATGA AGGAACTTCT AAACAGGAGT 
GTGTCGAAGG GCAGCTCCCA GGGCTCCTCT CTTCACAGCA ACTCTATCAG CTCTCAAGGA 
AACCACAAGA AAAACAGCAA CACAAAAGCT GATATGGAAA TACAGAATCA GACCCTGGCT 
AACGTAGTCG CAGAAAACCT GCAAGAAGAA GAGGAGGACA CCATGACAAG GACTACATGT 
CAGAGTCATG TGATAGAGTC TTGCTGCCAG ATGAGGACGT TGGACAGCGG GATTGGAACC 
TTCCCACTCC CCGACTCAGG GAACCGATCT GCGGGGCGAC ACGTGTCTAA GCAGGAATTA 
ACCTTGGAAA CAGAAGCCCT AGCACCGTCT GAGCAAGTTC TTCTTTCAGC TCCTTCAGTA 
AAAGCCAAAA CTCTTGAGCG AGAAGTGCCT TCAACAGCTA CGAGTCAGGA GTCTGTGGAA 
AGCATGATTA GTCACTCAAC ATCTGATCCA GCCATGACTG CCAAAGGTAT ACGACCTTTT 
CAAAGTCGCC TTCCAAAACC AGATTCCTCA GGAATAATTA ACCTTGCAAA GCAAAGTGAA 
CAAGAACCAA GTTCTGTGAC CTCTACCTCA TTACAGCTTA CTGAAGAAAC TGTAGAAAAT 
AGAAAAGTTC TTTCAGATTG GACCACCAAG AAAACTACAA AAGCAAAGGA CAGAACTCTA 
AGAGTGTGCA CTTACTCTGC CAGCAGCAGT GACGCTGAAC CGGAGCCAGA GTATGGAACA 
AATTATTTTC AAACTGCAGA GGAGATGTTA GTAGAGTTAA CAAAGAGCAA CAAACAAGCT 
GAGCAGGAAA AACAGAAGCA GAGAAAGTCA GTTTTGGGGA ACCCGATGTC AATCTTGGAT 
TTATACCAGC ACAGTCTCTA TGGCCATTTT GGAGAGGATG GACCTGAACA ATTAACACAT 
TTCAGTTTAA TTGAGCAGCT GAGTGAACCT TCCGGTAAAG ACAGCAAGGA CAAAGAGAGC 
CCAAGTAACC TGAAGCAAAC TGAAGAAACA AAAGAAGATC CTCAGACTAG ATTATCTAAA 
ATTTCCCTGG AGTCTCTCAA TAAATTTAAC AGCAATAACG TCCTTTTATT AGAAAAGGAG 
AAGAACTGTC TGAACAAAGC TGAAGGACAA AAGGAAGAAA GCGGAAAGAA GGAAGCATCT 
TCCTTAAACA GCTCCGATAG GCAGGACATG GACAATTTGG AGTCCCTGAG CGATTCTTTG 
TACGACAGCT TCTCCTCTTG TGCCAGCCAA GGCTCTAACG ACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_015289930.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145416.1 Gallus gallus NCK-associated protein 5 (NCKAP5), transcript variant X4, mRNA.

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CloneID	OGa38638
Clone ID Related Accession (Same CDS sequence)	XM_015289930.1
Accession Version	XM_015289930.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	nck-associated protein 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAC AACTCTTTGA CCTCGGGCTG ACGAGGCGGG CTCTCCAGAA GCAATCTTCC 
CAGCACGGCA GGATTTACGA GGCACGCCAG CCGGCCAGAC CCAACGGTGC GCTGCCCCCT 
CAGCAGGCCG CGTCTCCTTC CCACGCTTCC GACGGCCCCG AGGCGGCACC TCCGCTCGGG 
GAGCGCCGCG GGCCGTACTG TGCGGCGCTG CCCCCGGGGG ATTTCGGCGG CGGGAGAGCC 
TCGCCAGGGG CAGCCCCGGC CCGGGACGAG CCGCCGGCCC CGGCCCCGCC GCCGCAGACA 
ATGGCCGCCC CCGCCCGACC CCCCCCCCAA CCCCCTCCCC GCCGCGGCGA GCCCCTGCTG 
CCGCCGCACG GTTGCCGGAG CCATTCAGTC GCGGCCGATC GCGACCGCGG CAGATGGGCA 
GGATCTGACG GCCCGGTGCT CCGCGGCTGC CCGGGGCCGG GGCGGGCGGC TCGGGCGGAG 
CCCCCCCCCC TCGCCCCCGC GCGAAGTGCC GCCCCCGCAT GCCGAGCGTC CCGCGGCCGC 
GGAGGGAGCG GGGCACTCCG CAGCGCGACG AAGCGGAGGA GGCGCGCGGC GGCCGGCGGC 
GGGGGGCAGC CCGGGGGGCG CGGCCCCGCA CCGCTCGTCG AGGCGGCGGC CGGCGCCGCG 
CTCCCCGGGC CCGGCGCGGG CCGCCCCCGG CCGCCGCAGC ATGCCCCGCC GGGCAGCAGG 
GAGAAGCTGT CTGTGGCTCG GCTGCAGCGA GAAGTTGCGC AGAGTAAGAG CGAAGGAACC 
ATGCGCGAGA AACTGATACA TGAGTTGGAG GAAGAAAGAC ATTTAAGACT TGAGAGTGAA 
AAACGATTAC GGGAGGTAAC ACTGGAGTCA GAGCGTAACA GAGCTCAGAT GCGTGGCCTA 
CAAGAGCAGT TTTCCAGAAT GGAGGAAACG GTGAGAAATC TACTACAGAG CCAAGGATCC 
CCAGGCCAGA ATGGAGAAGG AACTGTCAAT ATCATGAAGG CATATCAGGA AAAGCTGTCA 
GAAGACAGTA GGAAATGCAA AGAAGTCATG GAAAAAAATC ACACAGCAGT AGATGAAGAT 
TCAAGAAATG AAAGCAACAG CACTGAAGAG GAAAAAGAAA AGACAAAATT GCTACTGGAG 
CGTTTGAAAG CTCTGGAGGC AGAGAATTCA GCACTGGCTC TGGAAAATGA AAATCAGAGG 
GAACAGTATG AAAGATGTCT TGATGAGGTA GCTAATCAGG TCGTCCAGGC TCTACTTACT 
CAAAAGGATC TGAGGGAGGA ATGTATAAAG CTGAAAACGA GAGTTTTTGA TTTGGAGCAA 
CAGAATCAAA CATTAAGTGT GCTTTTCCAG CAGAGAGTCA AACCAGCTTC TGACCTCCTC 
CTTCAGAAAC TCCATTCGCG CATCTTAGAC CTCTCATCTG GAGATTTGCT GTCAGAAGTA 
GATCGAAACA GAAGCTCGGT GCCGTCACGA CCTGCTGATG CTCAGATTCA CGAATGCCAG 
CAGAATGTGA AATCCACTAT ACCTGTCTTG AAATGCCACA GTCAATTAAA TGTGACAGGT 
CCTAGCCGCC TGTATCCCCG GAGCAGCTGC AGTAGCAGTG AACTCTCCCT TTCAAGTGCT 
TGCAGTGAAT ATTCCAGTGG TTCCTCCTAC ACTTGGAATG ATGGGAAGAC GTGCAGCAAA 
AGGTCATCCG TGAGCTGGGA TAAAAGAATA AGCATTGGTT CTTCACTCCC AAGCAACCTC 
TCTAGTCCTG CAGATGATCT GCCTCCAACT CGTATCAAGG AAAACCATAT CCTAGAAGGA 
CTGAAGAAAT TACAGAGGCA AAAAGTATTA CTTGAATCAC CTTCAGTAAT ACCGAAATGG 
GGTTACAAAG ATTGCATGAA CTCCAATGAA GGAATATATT CCCCTGGAGT TAAATGTGGC 
AGCCATCATG AGCAGACTCA TTGTAAGCCA ATGGAAATAG GAAGTAGTTG CATTGAACAT 
AACAAAACTT TCTCTTATGA TTCAGACTCA CATGATGATG CAGATGATGA TTCTTCATCT 
TTATTATTGC TGCAGGAAGT ACCAAGCAAA GACTGCAGGA TCTATTGTAA CAAATTAACT 
CACAGTGTTT CTGACAGTCT CTTTCACTGG GATCCTAATA GGAAACATTT GCCAGAAAGA 
AGTTCATATT TTAATTCAAA GGAAAGACCA GAAAAATTGA CCAGTTTTGT CAGTGGATTT 
CAGGCAGAAG TAGAATCGTG CACCAGAACA AAGCTGCCTG ATTTACAGTT AGATCAAAGC 
CATGCTAATA TAGGCTGGAA GGACCTTAAT TTTCAGCTTT CAGATACTGA TGACAATGAA 
GTCTTGGATG AATTGCATAT AGAAAGCAGT GATGAGAAAA GTCCATCAGA TTTATTAGCA 
ACTCCTTTTA CTGAGAAGTA CACAAAGAAC TCTGATGTGC TCATAGTCAC AGAGAATTCT 
CAGTTTATAT CTAGTGACAA AGAGGAAAGA CAGACATCTG CTCACTCAGA CATTAGGCCA 
AAGACATGTA ATTTCATTAA ACAACAAAAA GTCATAAAAA AGACTTCTTC TGAGGAGTGC 
ATTACAGTGA TATTTGATGC TGAGGACGGT GAGCCTATTG AATTCAGCTC GCATCAAATT 
GGAGTTGTCA CTGTAACAAG AAATGAGATA TCAGTCAACC AACCAGAAAG TAGGTCCAGT 
GCAGAATATA CTGAACTTAT ACCTCAGGGA ATAGCTAATT TGCAGGCAGG AACAAATGCT 
AGAGACTACA GTGTTTTGGA AGGACCTGAA GACAAAACTA AAAAACAGAC TCTTGAAGAT 
AATACAGGGA ATAAAAATAT GGTTGTCACC ACAACCTGCA GTGAATCTTC ACCCTGTGGA 
AGAATCATAC TGCAAAATGC ACCACGACAA AAACTAGTGA AGCCAGTGTA TGATGTGTCG 
TACAAGACCA ATTCAAGCTC CATTGTTCAT GCAGGAGTCA ATCAAAAGCC AATCTTGACT 
AAAATACCCA GCAGAGGTAA ATTTTCTCCA CAAAAAAACA AGATGACAGA GAGTGAGGAG 
GCACCTGTAG CTACTTCAGT GACCCCTGCA ACCCTTGAAA AATCACCTGC TCTTCCACCT 
GTAAAGCTTT CAAGACTCAA AAAGGCACAA AGTCCACCTC GTAATTTTGA CTCAAAAGTT 
GACTTCCAAG TTCCAAAGCC CCCTGCCCAC CCCCTGCCCA GCTCAAAGCG TCCGAGCAGA 
GGCAATGGGA CAAAGTATCC AAAGAGTCAG AGTCCAGCAT CACCACTGTT GCCTCAGCAC 
CTTATAGAGC CTACTGACTA TGGAGAACCT CCAACTAGAG ACTCTAATCA TGATTTAGCA 
ACTGTAGAAG CCAGGTCGCC ATCCCCACCC CTTCCTCCCG GCAGGTCAAC GTCCCTATTG 
ATTCGTCCTG ATTATGCTCA TTCACAACCT GCGGCTGTGA AGTTAGGAGG AGCTGTTCCA 
GGTGAAACAG CAAAAAATAT ACTGAAACCA TCACCATTAA AAGGAACTGC AAACTCTGGC 
ATCCATAATC AATCTAGTCA TGATATGCAA GCAAAAAGTC TATCTTCTGG GGCCAAAATT 
GAGTTATCTT ACCTTCAAGA AAATTCTGAA GCAATTATGC AAAATAAATG TATATCTCAG 
CAACCCCATA GTGCATGCCA AGGACTTTCC AAAGTTTCCA CAAAGCAAGT CTGCCTGAAA 
AACACCAATC AGATGGGTCT CCACAGGAAC CGTGAGTTTC TGAGCATGGA TTCTCAGACA 
GGCAGGTCCA TTCCTCTGGG TCCGTCACTG GAAACAACTT CACAAGAAGT GCATTCCAGC 
AAGAAGGATA TTTCCAGTGA CAGTGGGGTT GATCAGCCAC AAAAGATGCC AAACATTCTC 
CCTGCAACTC CTCAATGTCA TACAGCAAGC GTGAGTGAGC CCTTGCTATC TCAGGTAAAC 
AATTCTCCAT TGTCAACACT GTTTCATGGT AGTGACACTG CAAATGCCAC ACAAAACTCT 
AATGAGAAAG GAGTGAAAAC CCGTCTCCCT GTAGGACTGA AATTATTTGT CAAATCTCCT 
CAACTTCTCC GAAAGAGCTC CACCGTCCCA GGGAAGCAGG AAAAAGATAG TATAAATGCA 
GCTTCCAAAA GCCACGTGAG TACAAGTAAG CACAAGCAAA ATGAACGTGT GGCCACCAAA 
GGTGCAATAG ATGCTGAATT AAAGGACTCT AAGTCTGATA CCGAAATAAA AGATAATTTT 
TCTTTGAGAC TTAGTGTGGA TACAGCATCA CCTCGTTCAC ATGAAAATGG CAGCTTGGTG 
GTAGACAGAG TAGATGAATT AGAAAGCAAA CTAGTTAAGA GATCTTTTTC TTCTAGCAAC 
AAGTCACACT TGAAACCAGC TCTAGGCATG AATGGAGCGA AAGCTCGAAG TCAGAGTTTC 
AGCGTTCATA CAGGAGAAAA GTCATCTACC CTTGTGACAG AAGGTCTTGG AAAAGTACGG 
ACTCAGATTA TTACAAACAC TTCTGACAGA GGAAATTCTC TAACAAGGCA GAACTCAGCC 
ATGGAAGGAC TTCAAATCAA GGCCATTCCT GGGTCAGGAG CAACACAAGA GACTCTTTCA 
AATACTTCAA AAAACACAGA AAATGCTTAC TCTAGACAAG GTTCTCTTGG AAGTAATAGC 
AATAACCAGC ATGGAAGCCC AAGCAAACTG CCATTCAGAT CATCTCCAAA AGTAGACTTA 
TTTCGAAACA CTTCAAAATA TGATGGAAAC AAATCATTTT CTATGAAAGA TGCACGGAGT 
CTATCTGTCC AGAGTGAGAA AAGCAGTGAA AATTTAAAAC ACGAAGCTCT AAATAAAAAG 
AGTGTTTTGC CAGCAGAATT GGAGTTAGAT ACATCAGCTA CTGTATCTTC CAAACAGATT 
TCATCAATTC ATAGCTTGGG TGGAATAAAA TGTCCGCATA AACTGGAAGC AATAAAATCA 
CAGAGACAGC AGATGTCTTT AAAGTTAGCA GAAGCCCCTC AGGTTACTGA TGTTTTTAGT 
TCACCTGCTC TGCAACCTAC AATAGAGGAG AAGGTCATGT TGTGCATCCA AGAAAATGTC 
CAGAAGGGTC AGGAACAAAA CAAATCTCCC ACTGTAGAGA CTAAACAAAA GACTGGGCCC 
TCTATAGCTA GTTGGTTTGG CTTTCGAAAG AGTAAGCTGC CAGCACTGAG CAGCAGGAAA 
CCTGACGTTC CTAAAACAAA GGTAGAAAAA AAAGAACCAA GAGTTTCAGG ATTTGGAATT 
AAGCAGTCGA AATTAGAGAG AAGAAAAGAA AAAAAGCAAA CTGAACAACA CTGTGAAGTA 
GAAAATGAAA TTAACAGGAA AACGGACAGC AGTGACTTTT TAGATGATGC TATGGAGTTT 
AAAATTTTGA AGCCATCACG AAACACGTCT GGCAAGATTG GATGTGAACA ACTCAGAGGT 
TCCTCCATAG CTACATATTC ATCAAAAGAC AGTTTTATGA AGGAACTTCT AAACAGAGTT 
GATAAGAAAG CAGCTCAGCA GACAGAAAGT GGATCAAATA ATGTTTCCTA CAGGAGTGTG 
TCGAAGGGCA GCTCCCAGGG CTCCTCTCTT CACAGCAACT CTATCAGCTC TCAAGGAAAC 
CACAAGAAAA ACAGCAACAC AAAAGCTGAT ATGGAAATAC AGAATCAGAC CCTGGCTAAC 
GTAGTCGCAG AAAACCTGCA AGAAGAAGAG GAGGACACCA TGACAAGGAC TACATGTCAG 
AGTCATGTGA TAGAGTCTTG CTGCCAGATG AGGACGTTGG ACAGCGGGAT TGGAACCTTC 
CCACTCCCCG ACTCAGGGAA CCGATCTGCG GGGCGACACG TGTCTAAGCA GGAATTAACC 
TTGGAAACAG AAGCCCTAGC ACCGTCTGAG CAAGTTCTTC TTTCAGCTCC TTCAGTAAAA 
GCCAAAACTC TTGAGCGAGA AGTGCCTTCA ACAGCTACGA GTCAGGAGTC TGTGGAAAGC 
ATGATTAGTC ACTCAACATC TGATCCAGCC ATGACTGCCA AAGGTATACG ACCTTTTCAA 
AGTCGCCTTC CAAAACCAGA TTCCTCAGGA ATAATTAACC TTGCAAAGCA AAGTGAACAA 
GAACCAAGTT CTGTGACCTC TACCTCATTA CAGCTTACTG AAGAAACTGT AGAAAATAGA 
AAAGTTCTTT CAGATTGGAC CACCAAGAAA ACTACAAAAG CAAAGGACAG AACTCTAAGA 
GTGTGCACTT ACTCTGCCAG CAGCAGTGAC GCTGAACCGG AGCCAGAGTA TGGAACAAAT 
TATTTTCAAA CTGCAGAGGA GATGTTAGTA GAGTTAACAA AGAGCAACAA ACAAGCTGAG 
CAGGAAAAAC AGAAGCAGAG AAAGTCAGTT TTGGGGAACC CGATGTCAAT CTTGGATTTA 
TACCAGCACA GTCTCTATGG CCATTTTGGA GAGGATGGAC CTGAACAATT AACACATTTC 
AGTTTAATTG AGCAGCTGAG TGAACCTTCC GGTAAAGACA GCAAGGACAA AGAGAGCCCA 
AGTGTGAAGC AAGTGAAGCT TCTTTCACTT CTGAGAGCTG TAGAAAAACA GCATGCAGCG 
GAGAGTGGTT CAGCAACAAG GAGGGTGATA ACATTTTCTA TAACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145416.1
CDS	5360..12007
Translation	MATQLFDLGLTRRALQKQSSQHGRIYEARQPARPNGALPPQQAASPSHASDGPEAAPPLGERRGPYCAALPPGDFGGGRASPGAAPARDEPPAPAPPPQTMAAPARPPPQPPPRRGEPLLPPHGCRSHSVAADRDRGRWAGSDGPVLRGCPGPGRAARAEPPPLAPARSAAPACRASRGRGGSGALRSATKRRRRAAAGGGGQPGGRGPAPLVEAAAGAALPGPGAGRPRPPQHAPPGSREKLSVARLQREVAQSKSEGTMREKLIHELEEERHLRLESEKRLREVTLESERNRAQMRGLQEQFSRMEETVRNLLQSQGSPGQNGEGTVNIMKAYQEKLSEDSRKCKEVMEKNHTAVDEDSRNESNSTEEEKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECIKLKTRVFDLEQQNQTLSVLFQQRVKPASDLLLQKLHSRILDLSSGDLLSEVDRNRSSVPSRPADAQIHECQQNVKSTIPVLKCHSQLNVTGPSRLYPRSSCSSSELSLSSACSEYSSGSSYTWNDGKTCSKRSSVSWDKRISIGSSLPSNLSSPADDLPPTRIKENHILEGLKKLQRQKVLLESPSVIPKWGYKDCMNSNEGIYSPGVKCGSHHEQTHCKPMEIGSSCIEHNKTFSYDSDSHDDADDDSSSLLLLQEVPSKDCRIYCNKLTHSVSDSLFHWDPNRKHLPERSSYFNSKERPEKLTSFVSGFQAEVESCTRTKLPDLQLDQSHANIGWKDLNFQLSDTDDNEVLDELHIESSDEKSPSDLLATPFTEKYTKNSDVLIVTENSQFISSDKEERQTSAHSDIRPKTCNFIKQQKVIKKTSSEECITVIFDAEDGEPIEFSSHQIGVVTVTRNEISVNQPESRSSAEYTELIPQGIANLQAGTNARDYSVLEGPEDKTKKQTLEDNTGNKNMVVTTTCSESSPCGRIILQNAPRQKLVKPVYDVSYKTNSSSIVHAGVNQKPILTKIPSRGKFSPQKNKMTESEEAPVATSVTPATLEKSPALPPVKLSRLKKAQSPPRNFDSKVDFQVPKPPAHPLPSSKRPSRGNGTKYPKSQSPASPLLPQHLIEPTDYGEPPTRDSNHDLATVEARSPSPPLPPGRSTSLLIRPDYAHSQPAAVKLGGAVPGETAKNILKPSPLKGTANSGIHNQSSHDMQAKSLSSGAKIELSYLQENSEAIMQNKCISQQPHSACQGLSKVSTKQVCLKNTNQMGLHRNREFLSMDSQTGRSIPLGPSLETTSQEVHSSKKDISSDSGVDQPQKMPNILPATPQCHTASVSEPLLSQVNNSPLSTLFHGSDTANATQNSNEKGVKTRLPVGLKLFVKSPQLLRKSSTVPGKQEKDSINAASKSHVSTSKHKQNERVATKGAIDAELKDSKSDTEIKDNFSLRLSVDTASPRSHENGSLVVDRVDELESKLVKRSFSSSNKSHLKPALGMNGAKARSQSFSVHTGEKSSTLVTEGLGKVRTQIITNTSDRGNSLTRQNSAMEGLQIKAIPGSGATQETLSNTSKNTENAYSRQGSLGSNSNNQHGSPSKLPFRSSPKVDLFRNTSKYDGNKSFSMKDARSLSVQSEKSSENLKHEALNKKSVLPAELELDTSATVSSKQISSIHSLGGIKCPHKLEAIKSQRQQMSLKLAEAPQVTDVFSSPALQPTIEEKVMLCIQENVQKGQEQNKSPTVETKQKTGPSIASWFGFRKSKLPALSSRKPDVPKTKVEKKEPRVSGFGIKQSKLERRKEKKQTEQHCEVENEINRKTDSSDFLDDAMEFKILKPSRNTSGKIGCEQLRGSSIATYSSKDSFMKELLNRVDKKAAQQTESGSNNVSYRSVSKGSSQGSSLHSNSISSQGNHKKNSNTKADMEIQNQTLANVVAENLQEEEEDTMTRTTCQSHVIESCCQMRTLDSGIGTFPLPDSGNRSAGRHVSKQELTLETEALAPSEQVLLSAPSVKAKTLEREVPSTATSQESVESMISHSTSDPAMTAKGIRPFQSRLPKPDSSGIINLAKQSEQEPSSVTSTSLQLTEETVENRKVLSDWTTKKTTKAKDRTLRVCTYSASSSDAEPEPEYGTNYFQTAEEMLVELTKSNKQAEQEKQKQRKSVLGNPMSILDLYQHSLYGHFGEDGPEQLTHFSLIEQLSEPSGKDSKDKESPSVKQVKLLSLLRAVEKQHAAESGSATRRVITFSIT

Target ORF information:

RefSeq Version	XM_015289930.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NCK-associated protein 5 (NCKAP5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289930.1

ORF Insert Sequence:

ATGGCCACAC AACTCTTTGA CCTCGGGCTG ACGAGGCGGG CTCTCCAGAA GCAATCTTCC 
CAGCACGGCA GGATTTACGA GGCACGCCAG CCGGCCAGAC CCAACGGTGC GCTGCCCCCT 
CAGCAGGCCG CGTCTCCTTC CCACGCTTCC GACGGCCCCG AGGCGGCACC TCCGCTCGGG 
GAGCGCCGCG GGCCGTACTG TGCGGCGCTG CCCCCGGGGG ATTTCGGCGG CGGGAGAGCC 
TCGCCAGGGG CAGCCCCGGC CCGGGACGAG CCGCCGGCCC CGGCCCCGCC GCCGCAGACA 
ATGGCCGCCC CCGCCCGACC CCCCCCCCAA CCCCCTCCCC GCCGCGGCGA GCCCCTGCTG 
CCGCCGCACG GTTGCCGGAG CCATTCAGTC GCGGCCGATC GCGACCGCGG CAGATGGGCA 
GGATCTGACG GCCCGGTGCT CCGCGGCTGC CCGGGGCCGG GGCGGGCGGC TCGGGCGGAG 
CCCCCCCCCC TCGCCCCCGC GCGAAGTGCC GCCCCCGCAT GCCGAGCGTC CCGCGGCCGC 
GGAGGGAGCG GGGCACTCCG CAGCGCGACG AAGCGGAGGA GGCGCGCGGC GGCCGGCGGC 
GGGGGGCAGC CCGGGGGGCG CGGCCCCGCA CCGCTCGTCG AGGCGGCGGC CGGCGCCGCG 
CTCCCCGGGC CCGGCGCGGG CCGCCCCCGG CCGCCGCAGC ATGCCCCGCC GGGCAGCAGG 
GAGAAGCTGT CTGTGGCTCG GCTGCAGCGA GAAGTTGCGC AGAGTAAGAG CGAAGGAACC 
ATGCGCGAGA AACTGATACA TGAGTTGGAG GAAGAAAGAC ATTTAAGACT TGAGAGTGAA 
AAACGATTAC GGGAGGTAAC ACTGGAGTCA GAGCGTAACA GAGCTCAGAT GCGTGGCCTA 
CAAGAGCAGT TTTCCAGAAT GGAGGAAACG GTGAGAAATC TACTACAGAG CCAAGGATCC 
CCAGGCCAGA ATGGAGAAGG AACTGTCAAT ATCATGAAGG CATATCAGGA AAAGCTGTCA 
GAAGACAGTA GGAAATGCAA AGAAGTCATG GAAAAAAATC ACACAGCAGT AGATGAAGAT 
TCAAGAAATG AAAGCAACAG CACTGAAGAG GAAAAAGAAA AGACAAAATT GCTACTGGAG 
CGTTTGAAAG CTCTGGAGGC AGAGAATTCA GCACTGGCTC TGGAAAATGA AAATCAGAGG 
GAACAGTATG AAAGATGTCT TGATGAGGTA GCTAATCAGG TCGTCCAGGC TCTACTTACT 
CAAAAGGATC TGAGGGAGGA ATGTATAAAG CTGAAAACGA GAGTTTTTGA TTTGGAGCAA 
CAGAATCAAA CATTAAGTGT GCTTTTCCAG CAGAGAGTCA AACCAGCTTC TGACCTCCTC 
CTTCAGAAAC TCCATTCGCG CATCTTAGAC CTCTCATCTG GAGATTTGCT GTCAGAAGTA 
GATCGAAACA GAAGCTCGGT GCCGTCACGA CCTGCTGATG CTCAGATTCA CGAATGCCAG 
CAGAATGTGA AATCCACTAT ACCTGTCTTG AAATGCCACA GTCAATTAAA TGTGACAGGT 
CCTAGCCGCC TGTATCCCCG GAGCAGCTGC AGTAGCAGTG AACTCTCCCT TTCAAGTGCT 
TGCAGTGAAT ATTCCAGTGG TTCCTCCTAC ACTTGGAATG ATGGGAAGAC GTGCAGCAAA 
AGGTCATCCG TGAGCTGGGA TAAAAGAATA AGCATTGGTT CTTCACTCCC AAGCAACCTC 
TCTAGTCCTG CAGATGATCT GCCTCCAACT CGTATCAAGG AAAACCATAT CCTAGAAGGA 
CTGAAGAAAT TACAGAGGCA AAAAGTATTA CTTGAATCAC CTTCAGTAAT ACCGAAATGG 
GGTTACAAAG ATTGCATGAA CTCCAATGAA GGAATATATT CCCCTGGAGT TAAATGTGGC 
AGCCATCATG AGCAGACTCA TTGTAAGCCA ATGGAAATAG GAAGTAGTTG CATTGAACAT 
AACAAAACTT TCTCTTATGA TTCAGACTCA CATGATGATG CAGATGATGA TTCTTCATCT 
TTATTATTGC TGCAGGAAGT ACCAAGCAAA GACTGCAGGA TCTATTGTAA CAAATTAACT 
CACAGTGTTT CTGACAGTCT CTTTCACTGG GATCCTAATA GGAAACATTT GCCAGAAAGA 
AGTTCATATT TTAATTCAAA GGAAAGACCA GAAAAATTGA CCAGTTTTGT CAGTGGATTT 
CAGGCAGAAG TAGAATCGTG CACCAGAACA AAGCTGCCTG ATTTACAGTT AGATCAAAGC 
CATGCTAATA TAGGCTGGAA GGACCTTAAT TTTCAGCTTT CAGATACTGA TGACAATGAA 
GTCTTGGATG AATTGCATAT AGAAAGCAGT GATGAGAAAA GTCCATCAGA TTTATTAGCA 
ACTCCTTTTA CTGAGAAGTA CACAAAGAAC TCTGATGTGC TCATAGTCAC AGAGAATTCT 
CAGTTTATAT CTAGTGACAA AGAGGAAAGA CAGACATCTG CTCACTCAGA CATTAGGCCA 
AAGACATGTA ATTTCATTAA ACAACAAAAA GTCATAAAAA AGACTTCTTC TGAGGAGTGC 
ATTACAGTGA TATTTGATGC TGAGGACGGT GAGCCTATTG AATTCAGCTC GCATCAAATT 
GGAGTTGTCA CTGTAACAAG AAATGAGATA TCAGTCAACC AACCAGAAAG TAGGTCCAGT 
GCAGAATATA CTGAACTTAT ACCTCAGGGA ATAGCTAATT TGCAGGCAGG AACAAATGCT 
AGAGACTACA GTGTTTTGGA AGGACCTGAA GACAAAACTA AAAAACAGAC TCTTGAAGAT 
AATACAGGGA ATAAAAATAT GGTTGTCACC ACAACCTGCA GTGAATCTTC ACCCTGTGGA 
AGAATCATAC TGCAAAATGC ACCACGACAA AAACTAGTGA AGCCAGTGTA TGATGTGTCG 
TACAAGACCA ATTCAAGCTC CATTGTTCAT GCAGGAGTCA ATCAAAAGCC AATCTTGACT 
AAAATACCCA GCAGAGGTAA ATTTTCTCCA CAAAAAAACA AGATGACAGA GAGTGAGGAG 
GCACCTGTAG CTACTTCAGT GACCCCTGCA ACCCTTGAAA AATCACCTGC TCTTCCACCT 
GTAAAGCTTT CAAGACTCAA AAAGGCACAA AGTCCACCTC GTAATTTTGA CTCAAAAGTT 
GACTTCCAAG TTCCAAAGCC CCCTGCCCAC CCCCTGCCCA GCTCAAAGCG TCCGAGCAGA 
GGCAATGGGA CAAAGTATCC AAAGAGTCAG AGTCCAGCAT CACCACTGTT GCCTCAGCAC 
CTTATAGAGC CTACTGACTA TGGAGAACCT CCAACTAGAG ACTCTAATCA TGATTTAGCA 
ACTGTAGAAG CCAGGTCGCC ATCCCCACCC CTTCCTCCCG GCAGGTCAAC GTCCCTATTG 
ATTCGTCCTG ATTATGCTCA TTCACAACCT GCGGCTGTGA AGTTAGGAGG AGCTGTTCCA 
GGTGAAACAG CAAAAAATAT ACTGAAACCA TCACCATTAA AAGGAACTGC AAACTCTGGC 
ATCCATAATC AATCTAGTCA TGATATGCAA GCAAAAAGTC TATCTTCTGG GGCCAAAATT 
GAGTTATCTT ACCTTCAAGA AAATTCTGAA GCAATTATGC AAAATAAATG TATATCTCAG 
CAACCCCATA GTGCATGCCA AGGACTTTCC AAAGTTTCCA CAAAGCAAGT CTGCCTGAAA 
AACACCAATC AGATGGGTCT CCACAGGAAC CGTGAGTTTC TGAGCATGGA TTCTCAGACA 
GGCAGGTCCA TTCCTCTGGG TCCGTCACTG GAAACAACTT CACAAGAAGT GCATTCCAGC 
AAGAAGGATA TTTCCAGTGA CAGTGGGGTT GATCAGCCAC AAAAGATGCC AAACATTCTC 
CCTGCAACTC CTCAATGTCA TACAGCAAGC GTGAGTGAGC CCTTGCTATC TCAGGTAAAC 
AATTCTCCAT TGTCAACACT GTTTCATGGT AGTGACACTG CAAATGCCAC ACAAAACTCT 
AATGAGAAAG GAGTGAAAAC CCGTCTCCCT GTAGGACTGA AATTATTTGT CAAATCTCCT 
CAACTTCTCC GAAAGAGCTC CACCGTCCCA GGGAAGCAGG AAAAAGATAG TATAAATGCA 
GCTTCCAAAA GCCACGTGAG TACAAGTAAG CACAAGCAAA ATGAACGTGT GGCCACCAAA 
GGTGCAATAG ATGCTGAATT AAAGGACTCT AAGTCTGATA CCGAAATAAA AGATAATTTT 
TCTTTGAGAC TTAGTGTGGA TACAGCATCA CCTCGTTCAC ATGAAAATGG CAGCTTGGTG 
GTAGACAGAG TAGATGAATT AGAAAGCAAA CTAGTTAAGA GATCTTTTTC TTCTAGCAAC 
AAGTCACACT TGAAACCAGC TCTAGGCATG AATGGAGCGA AAGCTCGAAG TCAGAGTTTC 
AGCGTTCATA CAGGAGAAAA GTCATCTACC CTTGTGACAG AAGGTCTTGG AAAAGTACGG 
ACTCAGATTA TTACAAACAC TTCTGACAGA GGAAATTCTC TAACAAGGCA GAACTCAGCC 
ATGGAAGGAC TTCAAATCAA GGCCATTCCT GGGTCAGGAG CAACACAAGA GACTCTTTCA 
AATACTTCAA AAAACACAGA AAATGCTTAC TCTAGACAAG GTTCTCTTGG AAGTAATAGC 
AATAACCAGC ATGGAAGCCC AAGCAAACTG CCATTCAGAT CATCTCCAAA AGTAGACTTA 
TTTCGAAACA CTTCAAAATA TGATGGAAAC AAATCATTTT CTATGAAAGA TGCACGGAGT 
CTATCTGTCC AGAGTGAGAA AAGCAGTGAA AATTTAAAAC ACGAAGCTCT AAATAAAAAG 
AGTGTTTTGC CAGCAGAATT GGAGTTAGAT ACATCAGCTA CTGTATCTTC CAAACAGATT 
TCATCAATTC ATAGCTTGGG TGGAATAAAA TGTCCGCATA AACTGGAAGC AATAAAATCA 
CAGAGACAGC AGATGTCTTT AAAGTTAGCA GAAGCCCCTC AGGTTACTGA TGTTTTTAGT 
TCACCTGCTC TGCAACCTAC AATAGAGGAG AAGGTCATGT TGTGCATCCA AGAAAATGTC 
CAGAAGGGTC AGGAACAAAA CAAATCTCCC ACTGTAGAGA CTAAACAAAA GACTGGGCCC 
TCTATAGCTA GTTGGTTTGG CTTTCGAAAG AGTAAGCTGC CAGCACTGAG CAGCAGGAAA 
CCTGACGTTC CTAAAACAAA GGTAGAAAAA AAAGAACCAA GAGTTTCAGG ATTTGGAATT 
AAGCAGTCGA AATTAGAGAG AAGAAAAGAA AAAAAGCAAA CTGAACAACA CTGTGAAGTA 
GAAAATGAAA TTAACAGGAA AACGGACAGC AGTGACTTTT TAGATGATGC TATGGAGTTT 
AAAATTTTGA AGCCATCACG AAACACGTCT GGCAAGATTG GATGTGAACA ACTCAGAGGT 
TCCTCCATAG CTACATATTC ATCAAAAGAC AGTTTTATGA AGGAACTTCT AAACAGAGTT 
GATAAGAAAG CAGCTCAGCA GACAGAAAGT GGATCAAATA ATGTTTCCTA CAGGAGTGTG 
TCGAAGGGCA GCTCCCAGGG CTCCTCTCTT CACAGCAACT CTATCAGCTC TCAAGGAAAC 
CACAAGAAAA ACAGCAACAC AAAAGCTGAT ATGGAAATAC AGAATCAGAC CCTGGCTAAC 
GTAGTCGCAG AAAACCTGCA AGAAGAAGAG GAGGACACCA TGACAAGGAC TACATGTCAG 
AGTCATGTGA TAGAGTCTTG CTGCCAGATG AGGACGTTGG ACAGCGGGAT TGGAACCTTC 
CCACTCCCCG ACTCAGGGAA CCGATCTGCG GGGCGACACG TGTCTAAGCA GGAATTAACC 
TTGGAAACAG AAGCCCTAGC ACCGTCTGAG CAAGTTCTTC TTTCAGCTCC TTCAGTAAAA 
GCCAAAACTC TTGAGCGAGA AGTGCCTTCA ACAGCTACGA GTCAGGAGTC TGTGGAAAGC 
ATGATTAGTC ACTCAACATC TGATCCAGCC ATGACTGCCA AAGGTATACG ACCTTTTCAA 
AGTCGCCTTC CAAAACCAGA TTCCTCAGGA ATAATTAACC TTGCAAAGCA AAGTGAACAA 
GAACCAAGTT CTGTGACCTC TACCTCATTA CAGCTTACTG AAGAAACTGT AGAAAATAGA 
AAAGTTCTTT CAGATTGGAC CACCAAGAAA ACTACAAAAG CAAAGGACAG AACTCTAAGA 
GTGTGCACTT ACTCTGCCAG CAGCAGTGAC GCTGAACCGG AGCCAGAGTA TGGAACAAAT 
TATTTTCAAA CTGCAGAGGA GATGTTAGTA GAGTTAACAA AGAGCAACAA ACAAGCTGAG 
CAGGAAAAAC AGAAGCAGAG AAAGTCAGTT TTGGGGAACC CGATGTCAAT CTTGGATTTA 
TACCAGCACA GTCTCTATGG CCATTTTGGA GAGGATGGAC CTGAACAATT AACACATTTC 
AGTTTAATTG AGCAGCTGAG TGAACCTTCC GGTAAAGACA GCAAGGACAA AGAGAGCCCA 
AGTGTGAAGC AAGTGAAGCT TCTTTCACTT CTGAGAGCTG TAGAAAAACA GCATGCAGCG 
GAGAGTGGTT CAGCAACAAG GAGGGTGATA ACATTTTCTA TAACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_015289931.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145417.1 Gallus gallus NCK-associated protein 5 (NCKAP5), transcript variant X5, mRNA.

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CloneID	OGa38639
Clone ID Related Accession (Same CDS sequence)	XM_015289931.1
Accession Version	XM_015289931.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	nck-associated protein 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAC AACTCTTTGA CCTCGGGCTG ACGAGGCGGG CTCTCCAGAA GCAATCTTCC 
CAGCACGGCA GGATTTACGA GGCACGCCAG CCGGCCAGAC CCAACGGTGC GCTGCCCCCT 
CAGCAGGCCG CGTCTCCTTC CCACGCTTCC GACGGCCCCG AGGCGGCACC TCCGCTCGGG 
GAGCGCCGCG GGCCGTACTG TGCGGCGCTG CCCCCGGGGG ATTTCGGCGG CGGGAGAGCC 
TCGCCAGGGG CAGCCCCGGC CCGGGACGAG CCGCCGGCCC CGGCCCCGCC GCCGCAGACA 
ATGGCCGCCC CCGCCCGACC CCCCCCCCAA CCCCCTCCCC GCCGCGGCGA GCCCCTGCTG 
CCGCCGCACG GTTGCCGGAG CCATTCAGTC GCGGCCGATC GCGACCGCGG CAGATGGGCA 
GGATCTGACG GCCCGGTGCT CCGCGGCTGC CCGGGGCCGG GGCGGGCGGC TCGGGCGGAG 
CCCCCCCCCC TCGCCCCCGC GCGAAGTGCC GCCCCCGCAT GCCGAGCGTC CCGCGGCCGC 
GGAGGGAGCG GGGCACTCCG CAGCGCGACG AAGCGGAGGA GGCGCGCGGC GGCCGGCGGC 
GGGGGGCAGC CCGGGGGGCG CGGCCCCGCA CCGCTCGTCG AGGCGGCGGC CGGCGCCGCG 
CTCCCCGGGC CCGGCGCGGG CCGCCCCCGG CCGCCGCAGC ATGCCCCGCC GGGCAGCAGG 
GAGAAGCTGT CTGTGGCTCG GCTGCAGCGA GAAGTTGCGC AGAGTAAGAG CGAAGGAACC 
ATGCGCGAGA AACTGATACA TGAGTTGGAG GAAGAAAGAC ATTTAAGACT TGAGAGTGAA 
AAACGATTAC GGGAGGTAAC ACTGGAGTCA GAGCGTAACA GAGCTCAGAT GCGTGGCCTA 
CAAGAGCAGT TTTCCAGAAT GGAGGAAACG GTGAGAAATC TACTACAGAG CCAAGGATCC 
CCAGGCCAGA ATGGAGAAGG AACTGTCAAT ATCATGAAGG CATATCAGGA AAAGCTGTCA 
GAAGACAGTA GGAAATGCAA AGAAGTCATG GAAAAAAATC ACACAGCAGT AGATGAAGAT 
TCAAGAAATG AAAGCAACAG CACTGAAGAG GAAAAAGAAA AGACAAAATT GCTACTGGAG 
CGTTTGAAAG CTCTGGAGGC AGAGAATTCA GCACTGGCTC TGGAAAATGA AAATCAGAGG 
GAACAGTATG AAAGATGTCT TGATGAGGTA GCTAATCAGG TCGTCCAGGC TCTACTTACT 
CAAAAGGATC TGAGGGAGGA ATGTATAAAG CTGAAAACGA GAGTTTTTGA TTTGGAGCAA 
CAGAATCAAA CATTAAGTGT GCTTTTCCAG CAGAGAGTCA AACCAGCTTC TGACCTCCTC 
CTTCAGAAAC TCCATTCGCG CATCTTAGAC CTCTCATCTG GAGATTTGCT GTCAGAAGTA 
GATCGAAACA GAAGCTCGGT GCCGTCACGA CCTGCTGATG CTCAGATTCA CGAATGCCAG 
CAGAATGTGA AATCCACTAT ACCTGTCTTG AAATGCCACA GTCAATTAAA TGTGACAGGT 
CCTAGCCGCC TGTATCCCCG GAGCAGCTGC AGTAGCAGTG AACTCTCCCT TTCAAGTGCT 
TGCAGTGAAT ATTCCAGTGG TTCCTCCTAC ACTTGGAATG ATGGGAAGAC GTGCAGCAAA 
AGGTCATCCG TGAGCTGGGA TAAAAGAATA AGCATTGGTT CTTCACTCCC AAGCAACCTC 
TCTAGTCCTG CAGATGATCT GCCTCCAACT CGTATCAAGG AAAACCATAT CCTAGAAGGA 
CTGAAGAAAT TACAGAGGCA AAAAGTATTA CTTGAATCAC CTTCAGTAAT ACCGAAATGG 
GGTTACAAAG ATTGCATGAA CTCCAATGAA GGAATATATT CCCCTGGAGT TAAATGTGGC 
AGCCATCATG AGCAGACTCA TTGTAAGCCA ATGGAAATAG GAAGTAGTTG CATTGAACAT 
AACAAAACTT TCTCTTATGA TTCAGACTCA CATGATGATG CAGATGATGA TTCTTCATCT 
TTATTATTGC TGCAGGAAGT ACCAAGCAAA GACTGCAGGA TCTATTGTAA CAAATTAACT 
CACAGTGTTT CTGACAGTCT CTTTCACTGG GATCCTAATA GGAAACATTT GCCAGAAAGA 
AGTTCATATT TTAATTCAAA GGAAAGACCA GAAAAATTGA CCAGTTTTGT CAGTGGATTT 
CAGGCAGAAG TAGAATCGTG CACCAGAACA AAGCTGCCTG ATTTACAGTT AGATCAAAGC 
CATGCTAATA TAGGCTGGAA GGACCTTAAT TTTCAGCTTT CAGATACTGA TGACAATGAA 
GTCTTGGATG AATTGCATAT AGAAAGCAGT GATGAGAAAA GTCCATCAGA TTTATTAGCA 
ACTCCTTTTA CTGAGAAGTA CACAAAGAAC TCTGATGTGC TCATAGTCAC AGAGAATTCT 
CAGTTTATAT CTAGTGACAA AGAGGAAAGA CAGACATCTG CTCACTCAGA CATTAGGCCA 
AAGACATGTA ATTTCATTAA ACAACAAAAA GTCATAAAAA AGACTTCTTC TGAGGAGTGC 
ATTACAGTGA TATTTGATGC TGAGGACGGT GAGCCTATTG AATTCAGCTC GCATCAAATT 
GGAGTTGTCA CTGTAACAAG AAATGAGATA TCAGTCAACC AACCAGAAAG TAGGTCCAGT 
GCAGAATATA CTGAACTTAT ACCTCAGGGA ATAGCTAATT TGCAGGCAGG AACAAATGCT 
AGAGACTACA GTGTTTTGGA AGGACCTGAA GACAAAACTA AAAAACAGAC TCTTGAAGAT 
AATACAGGGA ATAAAAATAT GGTTGTCACC ACAACCTGCA GTGAATCTTC ACCCTGTGGA 
AGAATCATAC TGCAAAATGC ACCACGACAA AAACTAGTGA AGCCAGTGTA TGATGTGTCG 
TACAAGACCA ATTCAAGCTC CATTGTTCAT GCAGGAGTCA ATCAAAAGCC AATCTTGACT 
AAAATACCCA GCAGAGGTAA ATTTTCTCCA CAAAAAAACA AGATGACAGA GAGTGAGGAG 
GCACCTGTAG CTACTTCAGT GACCCCTGCA ACCCTTGAAA AATCACCTGC TCTTCCACCT 
GTAAAGCTTT CAAGACTCAA AAAGGCACAA AGTCCACCTC GTAATTTTGA CTCAAAAGTT 
GACTTCCAAG TTCCAAAGCC CCCTGCCCAC CCCCTGCCCA GCTCAAAGCG TCCGAGCAGA 
GGCAATGGGA CAAAGTATCC AAAGAGTCAG AGTCCAGCAT CACCACTGTT GCCTCAGCAC 
CTTATAGAGC CTACTGACTA TGGAGAACCT CCAACTAGAG ACTCTAATCA TGATTTAGCA 
ACTGTAGAAG CCAGGTCGCC ATCCCCACCC CTTCCTCCCG GCAGGTCAAC GTCCCTATTG 
ATTCGTCCTG ATTATGCTCA TTCACAACCT GCGGCTGTGA AGTTAGGAGG AGCTGTTCCA 
GGTGAAACAG CAAAAAATAT ACTGAAACCA TCACCATTAA AAGGAACTGC AAACTCTGGC 
ATCCATAATC AATCTAGTCA TGATATGCAA GCAAAAAGTC TATCTTCTGG GGCCAAAATT 
GAGTTATCTT ACCTTCAAGA AAATTCTGAA GCAATTATGC AAAATAAATG TATATCTCAG 
CAACCCCATA GTGCATGCCA AGGACTTTCC AAAGTTTCCA CAAAGCAAGT CTGCCTGAAA 
AACACCAATC AGATGGGTCT CCACAGGAAC CGTGAGTTTC TGAGCATGGA TTCTCAGACA 
GGCAGGTCCA TTCCTCTGGG TCCGTCACTG GAAACAACTT CACAAGAAGT GCATTCCAGC 
AAGAAGGATA TTTCCAGTGA CAGTGGGGTT GATCAGCCAC AAAAGATGCC AAACATTCTC 
CCTGCAACTC CTCAATGTCA TACAGCAAGC GTGAGTGAGC CCTTGCTATC TCAGGTAAAC 
AATTCTCCAT TGTCAACACT GTTTCATGGT AGTGACACTG CAAATGCCAC ACAAAACTCT 
AATGAGAAAG GAGTGAAAAC CCGTCTCCCT GTAGGACTGA AATTATTTGT CAAATCTCCT 
CAACTTCTCC GAAAGAGCTC CACCGTCCCA GGGAAGCAGG AAAAAGATAG TATAAATGCA 
GCTTCCAAAA GCCACGTGAG TACAAGTAAG CACAAGCAAA ATGAACGTGT GGCCACCAAA 
GGTGCAATAG ATGCTGAATT AAAGGACTCT AAGTCTGATA CCGAAATAAA AGATAATTTT 
TCTTTGAGAC TTAGTGTGGA TACAGCATCA CCTCGTTCAC ATGAAAATGG CAGCTTGGTG 
GTAGACAGAG TAGATGAATT AGAAAGCAAA CTAGTTAAGA GATCTTTTTC TTCTAGCAAC 
AAGTCACACT TGAAACCAGC TCTAGGCATG AATGGAGCGA AAGCTCGAAG TCAGAGTTTC 
AGCGTTCATA CAGGAGAAAA GTCATCTACC CTTGTGACAG AAGGTCTTGG AAAAGTACGG 
ACTCAGATTA TTACAAACAC TTCTGACAGA GGAAATTCTC TAACAAGGCA GAACTCAGCC 
ATGGAAGGAC TTCAAATCAA GGCCATTCCT GGGTCAGGAG CAACACAAGA GACTCTTTCA 
AATACTTCAA AAAACACAGA AAATGCTTAC TCTAGACAAG GTTCTCTTGG AAGTAATAGC 
AATAACCAGC ATGGAAGCCC AAGCAAACTG CCATTCAGAT CATCTCCAAA AGTAGACTTA 
TTTCGAAACA CTTCAAAATA TGATGGAAAC AAATCATTTT CTATGAAAGA TGCACGGAGT 
CTATCTGTCC AGAGTGAGAA AAGCAGTGAA AATTTAAAAC ACGAAGCTCT AAATAAAAAG 
AGTGTTTTGC CAGCAGAATT GGAGTTAGAT ACATCAGCTA CTGTATCTTC CAAACAGATT 
TCATCAATTC ATAGCTTGGG TGGAATAAAA TGTCCGCATA AACTGGAAGC AATAAAATCA 
CAGAGACAGC AGATGTCTTT AAAGTTAGCA GAAGCCCCTC AGGTTACTGA TGTTTTTAGT 
TCACCTGCTC TGCAACCTAC AATAGAGGAG AAGGTCATGT TGTGCATCCA AGAAAATGTC 
CAGAAGGGTC AGGAACAAAA CAAATCTCCC ACTGTAGAGA CTAAACAAAA GACTGGGCCC 
TCTATAGCTA GTTGGTTTGG CTTTCGAAAG AGTAAGCTGC CAGCACTGAG CAGCAGGAAA 
CCTGACGTTC CTAAAACAAA GGTAGAAAAA AAAGAACCAA GAGTTTCAGG ATTTGGAATT 
AAGCAGTCGA AATTAGAGAG AAGAAAAGAA AAAAAGCAAA CTGAACAACA CTGTGAAGTA 
GAAAATGAAA TTAACAGGAA AACGGACAGC AGTGACTTTT TAGATGATGC TATGGAGTTT 
AAAATTTTGA AGCCATCACG AAACACGTCT GGCAAGATTG GATGTGAACA ACTCAGAGGT 
TCCTCCATAG CTACATATTC ATCAAAAGAC AGTTTTATGA AGGAACTTCT AAACAGAGTT 
GATAAGAAAG CAGCTCAGCA GACAGAAAGT GGATCAAATA ATGTTTCCTA CAGGAGTGTG 
TCGAAGGGCA GCTCCCAGGG CTCCTCTCTT CACAGCAACT CTATCAGCTC TCAAGGAAAC 
CACAAGAAAA ACAGCAACAC AAAAGCTGAT ATGGAAATAC AGAATCAGAC CCTGGCTAAC 
GTAGTCGCAG AAAACCTGCA AGAAGAAGAG GAGGACACCA TGACAAGGAC TACATGTCAG 
AGTCATGTGA TAGAGTCTTG CTGCCAGATG AGGACGTTGG ACAGCGGGAT TGGAACCTTC 
CCACTCCCCG ACTCAGGGAA CCGATCTGCG GGGCGACACG TGTCTAAGCA GGAATTAACC 
TTGGAAACAG AAGCCCTAGC ACCGTCTGAG CAAGTTCTTC TTTCAGCTCC TTCAGTAAAA 
GCCAAAACTC TTGAGCGAGA AGTGCCTTCA ACAGCTACGA GTCAGGAGTC TGTGGAAAGC 
ATGATTAGTC ACTCAACATC TGATCCAGCC ATGACTGCCA AAGGTATACG ACCTTTTCAA 
AGTCGCCTTC CAAAACCAGA TTCCTCAGGA ATAATTAACC TTGCAAAGCA AAGTGAACAA 
GAACCAAGTT CTGTGACCTC TACCTCATTA CAGCTTACTG AAGAAACTGT AGAAAATAGA 
AAAGTTCTTT CAGATTGGAC CACCAAGAAA ACTACAAAAG CAAAGAACTC TAAGAGTGTG 
CACTTACTCT GCCAGCAGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145417.1
CDS	5363..11626
Translation	MATQLFDLGLTRRALQKQSSQHGRIYEARQPARPNGALPPQQAASPSHASDGPEAAPPLGERRGPYCAALPPGDFGGGRASPGAAPARDEPPAPAPPPQTMAAPARPPPQPPPRRGEPLLPPHGCRSHSVAADRDRGRWAGSDGPVLRGCPGPGRAARAEPPPLAPARSAAPACRASRGRGGSGALRSATKRRRRAAAGGGGQPGGRGPAPLVEAAAGAALPGPGAGRPRPPQHAPPGSREKLSVARLQREVAQSKSEGTMREKLIHELEEERHLRLESEKRLREVTLESERNRAQMRGLQEQFSRMEETVRNLLQSQGSPGQNGEGTVNIMKAYQEKLSEDSRKCKEVMEKNHTAVDEDSRNESNSTEEEKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECIKLKTRVFDLEQQNQTLSVLFQQRVKPASDLLLQKLHSRILDLSSGDLLSEVDRNRSSVPSRPADAQIHECQQNVKSTIPVLKCHSQLNVTGPSRLYPRSSCSSSELSLSSACSEYSSGSSYTWNDGKTCSKRSSVSWDKRISIGSSLPSNLSSPADDLPPTRIKENHILEGLKKLQRQKVLLESPSVIPKWGYKDCMNSNEGIYSPGVKCGSHHEQTHCKPMEIGSSCIEHNKTFSYDSDSHDDADDDSSSLLLLQEVPSKDCRIYCNKLTHSVSDSLFHWDPNRKHLPERSSYFNSKERPEKLTSFVSGFQAEVESCTRTKLPDLQLDQSHANIGWKDLNFQLSDTDDNEVLDELHIESSDEKSPSDLLATPFTEKYTKNSDVLIVTENSQFISSDKEERQTSAHSDIRPKTCNFIKQQKVIKKTSSEECITVIFDAEDGEPIEFSSHQIGVVTVTRNEISVNQPESRSSAEYTELIPQGIANLQAGTNARDYSVLEGPEDKTKKQTLEDNTGNKNMVVTTTCSESSPCGRIILQNAPRQKLVKPVYDVSYKTNSSSIVHAGVNQKPILTKIPSRGKFSPQKNKMTESEEAPVATSVTPATLEKSPALPPVKLSRLKKAQSPPRNFDSKVDFQVPKPPAHPLPSSKRPSRGNGTKYPKSQSPASPLLPQHLIEPTDYGEPPTRDSNHDLATVEARSPSPPLPPGRSTSLLIRPDYAHSQPAAVKLGGAVPGETAKNILKPSPLKGTANSGIHNQSSHDMQAKSLSSGAKIELSYLQENSEAIMQNKCISQQPHSACQGLSKVSTKQVCLKNTNQMGLHRNREFLSMDSQTGRSIPLGPSLETTSQEVHSSKKDISSDSGVDQPQKMPNILPATPQCHTASVSEPLLSQVNNSPLSTLFHGSDTANATQNSNEKGVKTRLPVGLKLFVKSPQLLRKSSTVPGKQEKDSINAASKSHVSTSKHKQNERVATKGAIDAELKDSKSDTEIKDNFSLRLSVDTASPRSHENGSLVVDRVDELESKLVKRSFSSSNKSHLKPALGMNGAKARSQSFSVHTGEKSSTLVTEGLGKVRTQIITNTSDRGNSLTRQNSAMEGLQIKAIPGSGATQETLSNTSKNTENAYSRQGSLGSNSNNQHGSPSKLPFRSSPKVDLFRNTSKYDGNKSFSMKDARSLSVQSEKSSENLKHEALNKKSVLPAELELDTSATVSSKQISSIHSLGGIKCPHKLEAIKSQRQQMSLKLAEAPQVTDVFSSPALQPTIEEKVMLCIQENVQKGQEQNKSPTVETKQKTGPSIASWFGFRKSKLPALSSRKPDVPKTKVEKKEPRVSGFGIKQSKLERRKEKKQTEQHCEVENEINRKTDSSDFLDDAMEFKILKPSRNTSGKIGCEQLRGSSIATYSSKDSFMKELLNRVDKKAAQQTESGSNNVSYRSVSKGSSQGSSLHSNSISSQGNHKKNSNTKADMEIQNQTLANVVAENLQEEEEDTMTRTTCQSHVIESCCQMRTLDSGIGTFPLPDSGNRSAGRHVSKQELTLETEALAPSEQVLLSAPSVKAKTLEREVPSTATSQESVESMISHSTSDPAMTAKGIRPFQSRLPKPDSSGIINLAKQSEQEPSSVTSTSLQLTEETVENRKVLSDWTTKKTTKAKNSKSVHLLCQQQ

Target ORF information:

RefSeq Version	XM_015289931.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NCK-associated protein 5 (NCKAP5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289931.1

ORF Insert Sequence:

ATGGCCACAC AACTCTTTGA CCTCGGGCTG ACGAGGCGGG CTCTCCAGAA GCAATCTTCC 
CAGCACGGCA GGATTTACGA GGCACGCCAG CCGGCCAGAC CCAACGGTGC GCTGCCCCCT 
CAGCAGGCCG CGTCTCCTTC CCACGCTTCC GACGGCCCCG AGGCGGCACC TCCGCTCGGG 
GAGCGCCGCG GGCCGTACTG TGCGGCGCTG CCCCCGGGGG ATTTCGGCGG CGGGAGAGCC 
TCGCCAGGGG CAGCCCCGGC CCGGGACGAG CCGCCGGCCC CGGCCCCGCC GCCGCAGACA 
ATGGCCGCCC CCGCCCGACC CCCCCCCCAA CCCCCTCCCC GCCGCGGCGA GCCCCTGCTG 
CCGCCGCACG GTTGCCGGAG CCATTCAGTC GCGGCCGATC GCGACCGCGG CAGATGGGCA 
GGATCTGACG GCCCGGTGCT CCGCGGCTGC CCGGGGCCGG GGCGGGCGGC TCGGGCGGAG 
CCCCCCCCCC TCGCCCCCGC GCGAAGTGCC GCCCCCGCAT GCCGAGCGTC CCGCGGCCGC 
GGAGGGAGCG GGGCACTCCG CAGCGCGACG AAGCGGAGGA GGCGCGCGGC GGCCGGCGGC 
GGGGGGCAGC CCGGGGGGCG CGGCCCCGCA CCGCTCGTCG AGGCGGCGGC CGGCGCCGCG 
CTCCCCGGGC CCGGCGCGGG CCGCCCCCGG CCGCCGCAGC ATGCCCCGCC GGGCAGCAGG 
GAGAAGCTGT CTGTGGCTCG GCTGCAGCGA GAAGTTGCGC AGAGTAAGAG CGAAGGAACC 
ATGCGCGAGA AACTGATACA TGAGTTGGAG GAAGAAAGAC ATTTAAGACT TGAGAGTGAA 
AAACGATTAC GGGAGGTAAC ACTGGAGTCA GAGCGTAACA GAGCTCAGAT GCGTGGCCTA 
CAAGAGCAGT TTTCCAGAAT GGAGGAAACG GTGAGAAATC TACTACAGAG CCAAGGATCC 
CCAGGCCAGA ATGGAGAAGG AACTGTCAAT ATCATGAAGG CATATCAGGA AAAGCTGTCA 
GAAGACAGTA GGAAATGCAA AGAAGTCATG GAAAAAAATC ACACAGCAGT AGATGAAGAT 
TCAAGAAATG AAAGCAACAG CACTGAAGAG GAAAAAGAAA AGACAAAATT GCTACTGGAG 
CGTTTGAAAG CTCTGGAGGC AGAGAATTCA GCACTGGCTC TGGAAAATGA AAATCAGAGG 
GAACAGTATG AAAGATGTCT TGATGAGGTA GCTAATCAGG TCGTCCAGGC TCTACTTACT 
CAAAAGGATC TGAGGGAGGA ATGTATAAAG CTGAAAACGA GAGTTTTTGA TTTGGAGCAA 
CAGAATCAAA CATTAAGTGT GCTTTTCCAG CAGAGAGTCA AACCAGCTTC TGACCTCCTC 
CTTCAGAAAC TCCATTCGCG CATCTTAGAC CTCTCATCTG GAGATTTGCT GTCAGAAGTA 
GATCGAAACA GAAGCTCGGT GCCGTCACGA CCTGCTGATG CTCAGATTCA CGAATGCCAG 
CAGAATGTGA AATCCACTAT ACCTGTCTTG AAATGCCACA GTCAATTAAA TGTGACAGGT 
CCTAGCCGCC TGTATCCCCG GAGCAGCTGC AGTAGCAGTG AACTCTCCCT TTCAAGTGCT 
TGCAGTGAAT ATTCCAGTGG TTCCTCCTAC ACTTGGAATG ATGGGAAGAC GTGCAGCAAA 
AGGTCATCCG TGAGCTGGGA TAAAAGAATA AGCATTGGTT CTTCACTCCC AAGCAACCTC 
TCTAGTCCTG CAGATGATCT GCCTCCAACT CGTATCAAGG AAAACCATAT CCTAGAAGGA 
CTGAAGAAAT TACAGAGGCA AAAAGTATTA CTTGAATCAC CTTCAGTAAT ACCGAAATGG 
GGTTACAAAG ATTGCATGAA CTCCAATGAA GGAATATATT CCCCTGGAGT TAAATGTGGC 
AGCCATCATG AGCAGACTCA TTGTAAGCCA ATGGAAATAG GAAGTAGTTG CATTGAACAT 
AACAAAACTT TCTCTTATGA TTCAGACTCA CATGATGATG CAGATGATGA TTCTTCATCT 
TTATTATTGC TGCAGGAAGT ACCAAGCAAA GACTGCAGGA TCTATTGTAA CAAATTAACT 
CACAGTGTTT CTGACAGTCT CTTTCACTGG GATCCTAATA GGAAACATTT GCCAGAAAGA 
AGTTCATATT TTAATTCAAA GGAAAGACCA GAAAAATTGA CCAGTTTTGT CAGTGGATTT 
CAGGCAGAAG TAGAATCGTG CACCAGAACA AAGCTGCCTG ATTTACAGTT AGATCAAAGC 
CATGCTAATA TAGGCTGGAA GGACCTTAAT TTTCAGCTTT CAGATACTGA TGACAATGAA 
GTCTTGGATG AATTGCATAT AGAAAGCAGT GATGAGAAAA GTCCATCAGA TTTATTAGCA 
ACTCCTTTTA CTGAGAAGTA CACAAAGAAC TCTGATGTGC TCATAGTCAC AGAGAATTCT 
CAGTTTATAT CTAGTGACAA AGAGGAAAGA CAGACATCTG CTCACTCAGA CATTAGGCCA 
AAGACATGTA ATTTCATTAA ACAACAAAAA GTCATAAAAA AGACTTCTTC TGAGGAGTGC 
ATTACAGTGA TATTTGATGC TGAGGACGGT GAGCCTATTG AATTCAGCTC GCATCAAATT 
GGAGTTGTCA CTGTAACAAG AAATGAGATA TCAGTCAACC AACCAGAAAG TAGGTCCAGT 
GCAGAATATA CTGAACTTAT ACCTCAGGGA ATAGCTAATT TGCAGGCAGG AACAAATGCT 
AGAGACTACA GTGTTTTGGA AGGACCTGAA GACAAAACTA AAAAACAGAC TCTTGAAGAT 
AATACAGGGA ATAAAAATAT GGTTGTCACC ACAACCTGCA GTGAATCTTC ACCCTGTGGA 
AGAATCATAC TGCAAAATGC ACCACGACAA AAACTAGTGA AGCCAGTGTA TGATGTGTCG 
TACAAGACCA ATTCAAGCTC CATTGTTCAT GCAGGAGTCA ATCAAAAGCC AATCTTGACT 
AAAATACCCA GCAGAGGTAA ATTTTCTCCA CAAAAAAACA AGATGACAGA GAGTGAGGAG 
GCACCTGTAG CTACTTCAGT GACCCCTGCA ACCCTTGAAA AATCACCTGC TCTTCCACCT 
GTAAAGCTTT CAAGACTCAA AAAGGCACAA AGTCCACCTC GTAATTTTGA CTCAAAAGTT 
GACTTCCAAG TTCCAAAGCC CCCTGCCCAC CCCCTGCCCA GCTCAAAGCG TCCGAGCAGA 
GGCAATGGGA CAAAGTATCC AAAGAGTCAG AGTCCAGCAT CACCACTGTT GCCTCAGCAC 
CTTATAGAGC CTACTGACTA TGGAGAACCT CCAACTAGAG ACTCTAATCA TGATTTAGCA 
ACTGTAGAAG CCAGGTCGCC ATCCCCACCC CTTCCTCCCG GCAGGTCAAC GTCCCTATTG 
ATTCGTCCTG ATTATGCTCA TTCACAACCT GCGGCTGTGA AGTTAGGAGG AGCTGTTCCA 
GGTGAAACAG CAAAAAATAT ACTGAAACCA TCACCATTAA AAGGAACTGC AAACTCTGGC 
ATCCATAATC AATCTAGTCA TGATATGCAA GCAAAAAGTC TATCTTCTGG GGCCAAAATT 
GAGTTATCTT ACCTTCAAGA AAATTCTGAA GCAATTATGC AAAATAAATG TATATCTCAG 
CAACCCCATA GTGCATGCCA AGGACTTTCC AAAGTTTCCA CAAAGCAAGT CTGCCTGAAA 
AACACCAATC AGATGGGTCT CCACAGGAAC CGTGAGTTTC TGAGCATGGA TTCTCAGACA 
GGCAGGTCCA TTCCTCTGGG TCCGTCACTG GAAACAACTT CACAAGAAGT GCATTCCAGC 
AAGAAGGATA TTTCCAGTGA CAGTGGGGTT GATCAGCCAC AAAAGATGCC AAACATTCTC 
CCTGCAACTC CTCAATGTCA TACAGCAAGC GTGAGTGAGC CCTTGCTATC TCAGGTAAAC 
AATTCTCCAT TGTCAACACT GTTTCATGGT AGTGACACTG CAAATGCCAC ACAAAACTCT 
AATGAGAAAG GAGTGAAAAC CCGTCTCCCT GTAGGACTGA AATTATTTGT CAAATCTCCT 
CAACTTCTCC GAAAGAGCTC CACCGTCCCA GGGAAGCAGG AAAAAGATAG TATAAATGCA 
GCTTCCAAAA GCCACGTGAG TACAAGTAAG CACAAGCAAA ATGAACGTGT GGCCACCAAA 
GGTGCAATAG ATGCTGAATT AAAGGACTCT AAGTCTGATA CCGAAATAAA AGATAATTTT 
TCTTTGAGAC TTAGTGTGGA TACAGCATCA CCTCGTTCAC ATGAAAATGG CAGCTTGGTG 
GTAGACAGAG TAGATGAATT AGAAAGCAAA CTAGTTAAGA GATCTTTTTC TTCTAGCAAC 
AAGTCACACT TGAAACCAGC TCTAGGCATG AATGGAGCGA AAGCTCGAAG TCAGAGTTTC 
AGCGTTCATA CAGGAGAAAA GTCATCTACC CTTGTGACAG AAGGTCTTGG AAAAGTACGG 
ACTCAGATTA TTACAAACAC TTCTGACAGA GGAAATTCTC TAACAAGGCA GAACTCAGCC 
ATGGAAGGAC TTCAAATCAA GGCCATTCCT GGGTCAGGAG CAACACAAGA GACTCTTTCA 
AATACTTCAA AAAACACAGA AAATGCTTAC TCTAGACAAG GTTCTCTTGG AAGTAATAGC 
AATAACCAGC ATGGAAGCCC AAGCAAACTG CCATTCAGAT CATCTCCAAA AGTAGACTTA 
TTTCGAAACA CTTCAAAATA TGATGGAAAC AAATCATTTT CTATGAAAGA TGCACGGAGT 
CTATCTGTCC AGAGTGAGAA AAGCAGTGAA AATTTAAAAC ACGAAGCTCT AAATAAAAAG 
AGTGTTTTGC CAGCAGAATT GGAGTTAGAT ACATCAGCTA CTGTATCTTC CAAACAGATT 
TCATCAATTC ATAGCTTGGG TGGAATAAAA TGTCCGCATA AACTGGAAGC AATAAAATCA 
CAGAGACAGC AGATGTCTTT AAAGTTAGCA GAAGCCCCTC AGGTTACTGA TGTTTTTAGT 
TCACCTGCTC TGCAACCTAC AATAGAGGAG AAGGTCATGT TGTGCATCCA AGAAAATGTC 
CAGAAGGGTC AGGAACAAAA CAAATCTCCC ACTGTAGAGA CTAAACAAAA GACTGGGCCC 
TCTATAGCTA GTTGGTTTGG CTTTCGAAAG AGTAAGCTGC CAGCACTGAG CAGCAGGAAA 
CCTGACGTTC CTAAAACAAA GGTAGAAAAA AAAGAACCAA GAGTTTCAGG ATTTGGAATT 
AAGCAGTCGA AATTAGAGAG AAGAAAAGAA AAAAAGCAAA CTGAACAACA CTGTGAAGTA 
GAAAATGAAA TTAACAGGAA AACGGACAGC AGTGACTTTT TAGATGATGC TATGGAGTTT 
AAAATTTTGA AGCCATCACG AAACACGTCT GGCAAGATTG GATGTGAACA ACTCAGAGGT 
TCCTCCATAG CTACATATTC ATCAAAAGAC AGTTTTATGA AGGAACTTCT AAACAGAGTT 
GATAAGAAAG CAGCTCAGCA GACAGAAAGT GGATCAAATA ATGTTTCCTA CAGGAGTGTG 
TCGAAGGGCA GCTCCCAGGG CTCCTCTCTT CACAGCAACT CTATCAGCTC TCAAGGAAAC 
CACAAGAAAA ACAGCAACAC AAAAGCTGAT ATGGAAATAC AGAATCAGAC CCTGGCTAAC 
GTAGTCGCAG AAAACCTGCA AGAAGAAGAG GAGGACACCA TGACAAGGAC TACATGTCAG 
AGTCATGTGA TAGAGTCTTG CTGCCAGATG AGGACGTTGG ACAGCGGGAT TGGAACCTTC 
CCACTCCCCG ACTCAGGGAA CCGATCTGCG GGGCGACACG TGTCTAAGCA GGAATTAACC 
TTGGAAACAG AAGCCCTAGC ACCGTCTGAG CAAGTTCTTC TTTCAGCTCC TTCAGTAAAA 
GCCAAAACTC TTGAGCGAGA AGTGCCTTCA ACAGCTACGA GTCAGGAGTC TGTGGAAAGC 
ATGATTAGTC ACTCAACATC TGATCCAGCC ATGACTGCCA AAGGTATACG ACCTTTTCAA 
AGTCGCCTTC CAAAACCAGA TTCCTCAGGA ATAATTAACC TTGCAAAGCA AAGTGAACAA 
GAACCAAGTT CTGTGACCTC TACCTCATTA CAGCTTACTG AAGAAACTGT AGAAAATAGA 
AAAGTTCTTT CAGATTGGAC CACCAAGAAA ACTACAAAAG CAAAGAACTC TAAGAGTGTG 
CACTTACTCT GCCAGCAGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_015289932.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145418.1 Gallus gallus NCK-associated protein 5 (NCKAP5), transcript variant X6, mRNA.

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CloneID	OGa38640
Clone ID Related Accession (Same CDS sequence)	XM_015289932.1 , XM_015289933.1 , XM_015289932.2 , XM_015289933.2
Accession Version	XM_015289932.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	nck-associated protein 5 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAA AGAGACTACT ACAAAAAAGG GAAATTGGGA AAAGACTTTC TCTGGACAGC 
AGCCTTGTGG ACTATATGGA CTCAAACAAA TACATAGAGC ATCTAGTGAC ACAGCTAGAG 
GAACAGCGCT GGAATTTATG GAGGGAGAAG CTGTCTGTGG CTCGGCTGCA GCGAGAAGTT 
GCGCAGAGTA AGAGCGAAGG AACCATGCGC GAGAAACTGA TACATGAGTT GGAGGAAGAA 
AGACATTTAA GACTTGAGAG TGAAAAACGA TTACGGGAGG TAACACTGGA GTCAGAGCGT 
AACAGAGCTC AGATGCGTGG CCTACAAGAG CAGTTTTCCA GAATGGAGGA AACGGTGAGA 
AATCTACTAC AGAGCCAAGG ATCCCCAGGC CAGAATGGAG AAGGAACTGT CAATATCATG 
AAGGCATATC AGGAAAAGCT GTCAGAAGAC AGTAGGAAAT GCAAAGAAGT CATGGAAAAA 
AATCACACAG CAGTAGATGA AGATTCAAGA AATGAAAGCA ACAGCACTGA AGAGGAAAAA 
GAAAAGACAA AATTGCTACT GGAGCGTTTG AAAGCTCTGG AGGCAGAGAA TTCAGCACTG 
GCTCTGGAAA ATGAAAATCA GAGGGAACAG TATGAAAGAT GTCTTGATGA GGTAGCTAAT 
CAGGTCGTCC AGGCTCTACT TACTCAAAAG GATCTGAGGG AGGAATGTAT AAAGCTGAAA 
ACGAGAGTTT TTGATTTGGA GCAACAGAAT CAAACATTAA GTGTGCTTTT CCAGCAGAGA 
GTCAAACCAG CTTCTGACCT CCTCCTTCAG AAACTCCATT CGCGCATCTT AGACCTCTCA 
TCTGGAGATT TGCTGTCAGA AGTAGATCGA AACAGAAGCT CGGTGCCGTC ACGACCTGCT 
GATGCTCAGA TTCACGAATG CCAGCAGAAT GTGAAATCCA CTATACCTGT CTTGAAATGC 
CACAGTCAAT TAAATGTGAC AGGTCCTAGC CGCCTGTATC CCCGGAGCAG CTGCAGTAGC 
AGTGAACTCT CCCTTTCAAG TGCTTGCAGT GAATATTCCA GTGGTTCCTC CTACACTTGG 
AATGATGGGA AGACGTGCAG CAAAAGGTCA TCCGTGAGCT GGGATAAAAG AATAAGCATT 
GGTTCTTCAC TCCCAAGCAA CCTCTCTAGT CCTGCAGATG ATCTGCCTCC AACTCGTATC 
AAGGAAAACC ATATCCTAGA AGGACTGAAG AAATTACAGA GGCAAAAAGT ATTACTTGAA 
TCACCTTCAG TAATACCGAA ATGGGGTTAC AAAGATTGCA TGAACTCCAA TGAAGGAATA 
TATTCCCCTG GAGTTAAATG TGGCAGCCAT CATGAGCAGA CTCATTGTAA GCCAATGGAA 
ATAGGAAGTA GTTGCATTGA ACATAACAAA ACTTTCTCTT ATGATTCAGA CTCACATGAT 
GATGCAGATG ATGATTCTTC ATCTTTATTA TTGCTGCAGG AAGTACCAAG CAAAGACTGC 
AGGATCTATT GTAACAAATT AACTCACAGT GTTTCTGACA GTCTCTTTCA CTGGGATCCT 
AATAGGAAAC ATTTGCCAGA AAGAAGTTCA TATTTTAATT CAAAGGAAAG ACCAGAAAAA 
TTGACCAGTT TTGTCAGTGG ATTTCAGGCA GAAGTAGAAT CGTGCACCAG AACAAAGCTG 
CCTGATTTAC AGTTAGATCA AAGCCATGCT AATATAGGCT GGAAGGACCT TAATTTTCAG 
CTTTCAGATA CTGATGACAA TGAAGTCTTG GATGAATTGC ATATAGAAAG CAGTGATGAG 
AAAAGTCCAT CAGATTTATT AGCAACTCCT TTTACTGAGA AGTACACAAA GAACTCTGAT 
GTGCTCATAG TCACAGAGAA TTCTCAGTTT ATATCTAGTG ACAAAGAGGA AAGACAGACA 
TCTGCTCACT CAGACATTAG GCCAAAGACA TGTAATTTCA TTAAACAACA AAAAGTCATA 
AAAAAGACTT CTTCTGAGGA GTGCATTACA GTGATATTTG ATGCTGAGGA CGGTGAGCCT 
ATTGAATTCA GCTCGCATCA AATTGGAGTT GTCACTGTAA CAAGAAATGA GATATCAGTC 
AACCAACCAG AAAGTAGGTC CAGTGCAGAA TATACTGAAC TTATACCTCA GGGAATAGCT 
AATTTGCAGG CAGGAACAAA TGCTAGAGAC TACAGTGTTT TGGAAGGACC TGAAGACAAA 
ACTAAAAAAC AGACTCTTGA AGATAATACA GGGAATAAAA ATATGGTTGT CACCACAACC 
TGCAGTGAAT CTTCACCCTG TGGAAGAATC ATACTGCAAA ATGCACCACG ACAAAAACTA 
GTGAAGCCAG TGTATGATGT GTCGTACAAG ACCAATTCAA GCTCCATTGT TCATGCAGGA 
GTCAATCAAA AGCCAATCTT GACTAAAATA CCCAGCAGAG GTAAATTTTC TCCACAAAAA 
AACAAGATGA CAGAGAGTGA GGAGGCACCT GTAGCTACTT CAGTGACCCC TGCAACCCTT 
GAAAAATCAC CTGCTCTTCC ACCTGTAAAG CTTTCAAGAC TCAAAAAGGC ACAAAGTCCA 
CCTCGTAATT TTGACTCAAA AGTTGACTTC CAAGTTCCAA AGCCCCCTGC CCACCCCCTG 
CCCAGCTCAA AGCGTCCGAG CAGAGGCAAT GGGACAAAGT ATCCAAAGAG TCAGAGTCCA 
GCATCACCAC TGTTGCCTCA GCACCTTATA GAGCCTACTG ACTATGGAGA ACCTCCAACT 
AGAGACTCTA ATCATGATTT AGCAACTGTA GAAGCCAGGT CGCCATCCCC ACCCCTTCCT 
CCCGGCAGGT CAACGTCCCT ATTGATTCGT CCTGATTATG CTCATTCACA ACCTGCGGCT 
GTGAAGTTAG GAGGAGCTGT TCCAGGTGAA ACAGCAAAAA ATATACTGAA ACCATCACCA 
TTAAAAGGAA CTGCAAACTC TGGCATCCAT AATCAATCTA GTCATGATAT GCAAGCAAAA 
AGTCTATCTT CTGGGGCCAA AATTGAGTTA TCTTACCTTC AAGAAAATTC TGAAGCAATT 
ATGCAAAATA AATGTATATC TCAGCAACCC CATAGTGCAT GCCAAGGACT TTCCAAAGTT 
TCCACAAAGC AAGTCTGCCT GAAAAACACC AATCAGATGG GTCTCCACAG GAACCGTGAG 
TTTCTGAGCA TGGATTCTCA GACAGGCAGG TCCATTCCTC TGGGTCCGTC ACTGGAAACA 
ACTTCACAAG AAGTGCATTC CAGCAAGAAG GATATTTCCA GTGACAGTGG GGTTGATCAG 
CCACAAAAGA TGCCAAACAT TCTCCCTGCA ACTCCTCAAT GTCATACAGC AAGCGTGAGT 
GAGCCCTTGC TATCTCAGGT AAACAATTCT CCATTGTCAA CACTGTTTCA TGGTAGTGAC 
ACTGCAAATG CCACACAAAA CTCTAATGAG AAAGGAGTGA AAACCCGTCT CCCTGTAGGA 
CTGAAATTAT TTGTCAAATC TCCTCAACTT CTCCGAAAGA GCTCCACCGT CCCAGGGAAG 
CAGGAAAAAG ATAGTATAAA TGCAGCTTCC AAAAGCCACG TGAGTACAAG TAAGCACAAG 
CAAAATGAAC GTGTGGCCAC CAAAGGTGCA ATAGATGCTG AATTAAAGGA CTCTAAGTCT 
GATACCGAAA TAAAAGATAA TTTTTCTTTG AGACTTAGTG TGGATACAGC ATCACCTCGT 
TCACATGAAA ATGGCAGCTT GGTGGTAGAC AGAGTAGATG AATTAGAAAG CAAACTAGTT 
AAGAGATCTT TTTCTTCTAG CAACAAGTCA CACTTGAAAC CAGCTCTAGG CATGAATGGA 
GCGAAAGCTC GAAGTCAGAG TTTCAGCGTT CATACAGGAG AAAAGTCATC TACCCTTGTG 
ACAGAAGGTC TTGGAAAAGT ACGGACTCAG ATTATTACAA ACACTTCTGA CAGAGGAAAT 
TCTCTAACAA GGCAGAACTC AGCCATGGAA GGACTTCAAA TCAAGGCCAT TCCTGGGTCA 
GGAGCAACAC AAGAGACTCT TTCAAATACT TCAAAAAACA CAGAAAATGC TTACTCTAGA 
CAAGGTTCTC TTGGAAGTAA TAGCAATAAC CAGCATGGAA GCCCAAGCAA ACTGCCATTC 
AGATCATCTC CAAAAGTAGA CTTATTTCGA AACACTTCAA AATATGATGG AAACAAATCA 
TTTTCTATGA AAGATGCACG GAGTCTATCT GTCCAGAGTG AGAAAAGCAG TGAAAATTTA 
AAACACGAAG CTCTAAATAA AAAGAGTGTT TTGCCAGCAG AATTGGAGTT AGATACATCA 
GCTACTGTAT CTTCCAAACA GATTTCATCA ATTCATAGCT TGGGTGGAAT AAAATGTCCG 
CATAAACTGG AAGCAATAAA ATCACAGAGA CAGCAGATGT CTTTAAAGTT AGCAGAAGCC 
CCTCAGGTTA CTGATGTTTT TAGTTCACCT GCTCTGCAAC CTACAATAGA GGAGAAGGTC 
ATGTTGTGCA TCCAAGAAAA TGTCCAGAAG GGTCAGGAAC AAAACAAATC TCCCACTGTA 
GAGACTAAAC AAAAGACTGG GCCCTCTATA GCTAGTTGGT TTGGCTTTCG AAAGAGTAAG 
CTGCCAGCAC TGAGCAGCAG GAAACCTGAC GTTCCTAAAA CAAAGGTAGA AAAAAAAGAA 
CCAAGAGTTT CAGGATTTGG AATTAAGCAG TCGAAATTAG AGAGAAGAAA AGAAAAAAAG 
CAAACTGAAC AACACTGTGA AGTAGAAAAT GAAATTAACA GGAAAACGGA CAGCAGTGAC 
TTTTTAGATG ATGCTATGGA GTTTAAAATT TTGAAGCCAT CACGAAACAC GTCTGGCAAG 
ATTGGATGTG AACAACTCAG AGGTTCCTCC ATAGCTACAT ATTCATCAAA AGACAGTTTT 
ATGAAGGAAC TTCTAAACAG AGTTGATAAG AAAGCAGCTC AGCAGACAGA AAGTGGATCA 
AATAATGTTT CCTACAGGAG TGTGTCGAAG GGCAGCTCCC AGGGCTCCTC TCTTCACAGC 
AACTCTATCA GCTCTCAAGG AAACCACAAG AAAAACAGCA ACACAAAAGC TGATATGGAA 
ATACAGAATC AGACCCTGGC TAACGTAGTC GCAGAAAACC TGCAAGAAGA AGAGGAGGAC 
ACCATGACAA GGACTACATG TCAGAGTCAT GTGATAGAGT CTTGCTGCCA GATGAGGACG 
TTGGACAGCG GGATTGGAAC CTTCCCACTC CCCGACTCAG GGAACCGATC TGCGGGGCGA 
CACGTGTCTA AGCAGGAATT AACCTTGGAA ACAGAAGCCC TAGCACCGTC TGAGCAAGTT 
CTTCTTTCAG CTCCTTCAGT AAAAGCCAAA ACTCTTGAGC GAGAAGTGCC TTCAACAGCT 
ACGAGTCAGG AGTCTGTGGA AAGCATGATT AGTCACTCAA CATCTGATCC AGCCATGACT 
GCCAAAGGTA TACGACCTTT TCAAAGTCGC CTTCCAAAAC CAGATTCCTC AGGAATAATT 
AACCTTGCAA AGCAAAGTGA ACAAGAACCA AGTTCTGTGA CCTCTACCTC ATTACAGCTT 
ACTGAAGAAA CTGTAGAAAA TAGAAAAGTT CTTTCAGATT GGACCACCAA GAAAACTACA 
AAAGCAAAGG ACAGAACTCT AAGAGTGTGC ACTTACTCTG CCAGCAGCAG TGACGCTGAA 
CCGGAGCCAG AGTATGGAAC AAATTATTTT CAAACTGCAG AGGAGATGTT AGTAGAGTTA 
ACAAAGAGCA ACAAACAAGC TGAGCAGGAA AAACAGAAGC AGAGAAAGTC AGTTTTGGGG 
AACCCGATGT CAATCTTGGA TTTATACCAG CACAGTCTCT ATGGCCATTT TGGAGAGGAT 
GGACCTGAAC AATTAACACA TTTCAGTTTA ATTGAGCAGC TGAGTGAACC TTCCGGTAAA 
GACAGCAAGG ACAAAGAGAG CCCAAGTAAC CTGAAGCAAA CTGAAGAAAC AAAAGAAGAT 
CCTCAGACTA GATTATCTAA AATTTCCCTG GAGTCTCTCA ATAAATTTAA CAGCAATAAC 
GTCCTTTTAT TAGAAAAGGA GAAGAACTGT CTGAACAAAG CTGAAGGACA AAAGGAAGAA 
AGCGGAAAGA AGGAAGCATC TTCCTTAAAC AGCTCCGATA GGCAGGACAT GGACAATTTG 
GAGTCCCTGA GCGATTCTTT GTACGACAGC TTCTCCTCTT GTGCCAGCCA AGGCTCTAAC 
GACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145418.1
CDS	290..6538
Translation	MERKRLLQKREIGKRLSLDSSLVDYMDSNKYIEHLVTQLEEQRWNLWREKLSVARLQREVAQSKSEGTMREKLIHELEEERHLRLESEKRLREVTLESERNRAQMRGLQEQFSRMEETVRNLLQSQGSPGQNGEGTVNIMKAYQEKLSEDSRKCKEVMEKNHTAVDEDSRNESNSTEEEKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECIKLKTRVFDLEQQNQTLSVLFQQRVKPASDLLLQKLHSRILDLSSGDLLSEVDRNRSSVPSRPADAQIHECQQNVKSTIPVLKCHSQLNVTGPSRLYPRSSCSSSELSLSSACSEYSSGSSYTWNDGKTCSKRSSVSWDKRISIGSSLPSNLSSPADDLPPTRIKENHILEGLKKLQRQKVLLESPSVIPKWGYKDCMNSNEGIYSPGVKCGSHHEQTHCKPMEIGSSCIEHNKTFSYDSDSHDDADDDSSSLLLLQEVPSKDCRIYCNKLTHSVSDSLFHWDPNRKHLPERSSYFNSKERPEKLTSFVSGFQAEVESCTRTKLPDLQLDQSHANIGWKDLNFQLSDTDDNEVLDELHIESSDEKSPSDLLATPFTEKYTKNSDVLIVTENSQFISSDKEERQTSAHSDIRPKTCNFIKQQKVIKKTSSEECITVIFDAEDGEPIEFSSHQIGVVTVTRNEISVNQPESRSSAEYTELIPQGIANLQAGTNARDYSVLEGPEDKTKKQTLEDNTGNKNMVVTTTCSESSPCGRIILQNAPRQKLVKPVYDVSYKTNSSSIVHAGVNQKPILTKIPSRGKFSPQKNKMTESEEAPVATSVTPATLEKSPALPPVKLSRLKKAQSPPRNFDSKVDFQVPKPPAHPLPSSKRPSRGNGTKYPKSQSPASPLLPQHLIEPTDYGEPPTRDSNHDLATVEARSPSPPLPPGRSTSLLIRPDYAHSQPAAVKLGGAVPGETAKNILKPSPLKGTANSGIHNQSSHDMQAKSLSSGAKIELSYLQENSEAIMQNKCISQQPHSACQGLSKVSTKQVCLKNTNQMGLHRNREFLSMDSQTGRSIPLGPSLETTSQEVHSSKKDISSDSGVDQPQKMPNILPATPQCHTASVSEPLLSQVNNSPLSTLFHGSDTANATQNSNEKGVKTRLPVGLKLFVKSPQLLRKSSTVPGKQEKDSINAASKSHVSTSKHKQNERVATKGAIDAELKDSKSDTEIKDNFSLRLSVDTASPRSHENGSLVVDRVDELESKLVKRSFSSSNKSHLKPALGMNGAKARSQSFSVHTGEKSSTLVTEGLGKVRTQIITNTSDRGNSLTRQNSAMEGLQIKAIPGSGATQETLSNTSKNTENAYSRQGSLGSNSNNQHGSPSKLPFRSSPKVDLFRNTSKYDGNKSFSMKDARSLSVQSEKSSENLKHEALNKKSVLPAELELDTSATVSSKQISSIHSLGGIKCPHKLEAIKSQRQQMSLKLAEAPQVTDVFSSPALQPTIEEKVMLCIQENVQKGQEQNKSPTVETKQKTGPSIASWFGFRKSKLPALSSRKPDVPKTKVEKKEPRVSGFGIKQSKLERRKEKKQTEQHCEVENEINRKTDSSDFLDDAMEFKILKPSRNTSGKIGCEQLRGSSIATYSSKDSFMKELLNRVDKKAAQQTESGSNNVSYRSVSKGSSQGSSLHSNSISSQGNHKKNSNTKADMEIQNQTLANVVAENLQEEEEDTMTRTTCQSHVIESCCQMRTLDSGIGTFPLPDSGNRSAGRHVSKQELTLETEALAPSEQVLLSAPSVKAKTLEREVPSTATSQESVESMISHSTSDPAMTAKGIRPFQSRLPKPDSSGIINLAKQSEQEPSSVTSTSLQLTEETVENRKVLSDWTTKKTTKAKDRTLRVCTYSASSSDAEPEPEYGTNYFQTAEEMLVELTKSNKQAEQEKQKQRKSVLGNPMSILDLYQHSLYGHFGEDGPEQLTHFSLIEQLSEPSGKDSKDKESPSNLKQTEETKEDPQTRLSKISLESLNKFNSNNVLLLEKEKNCLNKAEGQKEESGKKEASSLNSSDRQDMDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_015289932.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NCK-associated protein 5 (NCKAP5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289932.1

ORF Insert Sequence:

ATGGAGAGAA AGAGACTACT ACAAAAAAGG GAAATTGGGA AAAGACTTTC TCTGGACAGC 
AGCCTTGTGG ACTATATGGA CTCAAACAAA TACATAGAGC ATCTAGTGAC ACAGCTAGAG 
GAACAGCGCT GGAATTTATG GAGGGAGAAG CTGTCTGTGG CTCGGCTGCA GCGAGAAGTT 
GCGCAGAGTA AGAGCGAAGG AACCATGCGC GAGAAACTGA TACATGAGTT GGAGGAAGAA 
AGACATTTAA GACTTGAGAG TGAAAAACGA TTACGGGAGG TAACACTGGA GTCAGAGCGT 
AACAGAGCTC AGATGCGTGG CCTACAAGAG CAGTTTTCCA GAATGGAGGA AACGGTGAGA 
AATCTACTAC AGAGCCAAGG ATCCCCAGGC CAGAATGGAG AAGGAACTGT CAATATCATG 
AAGGCATATC AGGAAAAGCT GTCAGAAGAC AGTAGGAAAT GCAAAGAAGT CATGGAAAAA 
AATCACACAG CAGTAGATGA AGATTCAAGA AATGAAAGCA ACAGCACTGA AGAGGAAAAA 
GAAAAGACAA AATTGCTACT GGAGCGTTTG AAAGCTCTGG AGGCAGAGAA TTCAGCACTG 
GCTCTGGAAA ATGAAAATCA GAGGGAACAG TATGAAAGAT GTCTTGATGA GGTAGCTAAT 
CAGGTCGTCC AGGCTCTACT TACTCAAAAG GATCTGAGGG AGGAATGTAT AAAGCTGAAA 
ACGAGAGTTT TTGATTTGGA GCAACAGAAT CAAACATTAA GTGTGCTTTT CCAGCAGAGA 
GTCAAACCAG CTTCTGACCT CCTCCTTCAG AAACTCCATT CGCGCATCTT AGACCTCTCA 
TCTGGAGATT TGCTGTCAGA AGTAGATCGA AACAGAAGCT CGGTGCCGTC ACGACCTGCT 
GATGCTCAGA TTCACGAATG CCAGCAGAAT GTGAAATCCA CTATACCTGT CTTGAAATGC 
CACAGTCAAT TAAATGTGAC AGGTCCTAGC CGCCTGTATC CCCGGAGCAG CTGCAGTAGC 
AGTGAACTCT CCCTTTCAAG TGCTTGCAGT GAATATTCCA GTGGTTCCTC CTACACTTGG 
AATGATGGGA AGACGTGCAG CAAAAGGTCA TCCGTGAGCT GGGATAAAAG AATAAGCATT 
GGTTCTTCAC TCCCAAGCAA CCTCTCTAGT CCTGCAGATG ATCTGCCTCC AACTCGTATC 
AAGGAAAACC ATATCCTAGA AGGACTGAAG AAATTACAGA GGCAAAAAGT ATTACTTGAA 
TCACCTTCAG TAATACCGAA ATGGGGTTAC AAAGATTGCA TGAACTCCAA TGAAGGAATA 
TATTCCCCTG GAGTTAAATG TGGCAGCCAT CATGAGCAGA CTCATTGTAA GCCAATGGAA 
ATAGGAAGTA GTTGCATTGA ACATAACAAA ACTTTCTCTT ATGATTCAGA CTCACATGAT 
GATGCAGATG ATGATTCTTC ATCTTTATTA TTGCTGCAGG AAGTACCAAG CAAAGACTGC 
AGGATCTATT GTAACAAATT AACTCACAGT GTTTCTGACA GTCTCTTTCA CTGGGATCCT 
AATAGGAAAC ATTTGCCAGA AAGAAGTTCA TATTTTAATT CAAAGGAAAG ACCAGAAAAA 
TTGACCAGTT TTGTCAGTGG ATTTCAGGCA GAAGTAGAAT CGTGCACCAG AACAAAGCTG 
CCTGATTTAC AGTTAGATCA AAGCCATGCT AATATAGGCT GGAAGGACCT TAATTTTCAG 
CTTTCAGATA CTGATGACAA TGAAGTCTTG GATGAATTGC ATATAGAAAG CAGTGATGAG 
AAAAGTCCAT CAGATTTATT AGCAACTCCT TTTACTGAGA AGTACACAAA GAACTCTGAT 
GTGCTCATAG TCACAGAGAA TTCTCAGTTT ATATCTAGTG ACAAAGAGGA AAGACAGACA 
TCTGCTCACT CAGACATTAG GCCAAAGACA TGTAATTTCA TTAAACAACA AAAAGTCATA 
AAAAAGACTT CTTCTGAGGA GTGCATTACA GTGATATTTG ATGCTGAGGA CGGTGAGCCT 
ATTGAATTCA GCTCGCATCA AATTGGAGTT GTCACTGTAA CAAGAAATGA GATATCAGTC 
AACCAACCAG AAAGTAGGTC CAGTGCAGAA TATACTGAAC TTATACCTCA GGGAATAGCT 
AATTTGCAGG CAGGAACAAA TGCTAGAGAC TACAGTGTTT TGGAAGGACC TGAAGACAAA 
ACTAAAAAAC AGACTCTTGA AGATAATACA GGGAATAAAA ATATGGTTGT CACCACAACC 
TGCAGTGAAT CTTCACCCTG TGGAAGAATC ATACTGCAAA ATGCACCACG ACAAAAACTA 
GTGAAGCCAG TGTATGATGT GTCGTACAAG ACCAATTCAA GCTCCATTGT TCATGCAGGA 
GTCAATCAAA AGCCAATCTT GACTAAAATA CCCAGCAGAG GTAAATTTTC TCCACAAAAA 
AACAAGATGA CAGAGAGTGA GGAGGCACCT GTAGCTACTT CAGTGACCCC TGCAACCCTT 
GAAAAATCAC CTGCTCTTCC ACCTGTAAAG CTTTCAAGAC TCAAAAAGGC ACAAAGTCCA 
CCTCGTAATT TTGACTCAAA AGTTGACTTC CAAGTTCCAA AGCCCCCTGC CCACCCCCTG 
CCCAGCTCAA AGCGTCCGAG CAGAGGCAAT GGGACAAAGT ATCCAAAGAG TCAGAGTCCA 
GCATCACCAC TGTTGCCTCA GCACCTTATA GAGCCTACTG ACTATGGAGA ACCTCCAACT 
AGAGACTCTA ATCATGATTT AGCAACTGTA GAAGCCAGGT CGCCATCCCC ACCCCTTCCT 
CCCGGCAGGT CAACGTCCCT ATTGATTCGT CCTGATTATG CTCATTCACA ACCTGCGGCT 
GTGAAGTTAG GAGGAGCTGT TCCAGGTGAA ACAGCAAAAA ATATACTGAA ACCATCACCA 
TTAAAAGGAA CTGCAAACTC TGGCATCCAT AATCAATCTA GTCATGATAT GCAAGCAAAA 
AGTCTATCTT CTGGGGCCAA AATTGAGTTA TCTTACCTTC AAGAAAATTC TGAAGCAATT 
ATGCAAAATA AATGTATATC TCAGCAACCC CATAGTGCAT GCCAAGGACT TTCCAAAGTT 
TCCACAAAGC AAGTCTGCCT GAAAAACACC AATCAGATGG GTCTCCACAG GAACCGTGAG 
TTTCTGAGCA TGGATTCTCA GACAGGCAGG TCCATTCCTC TGGGTCCGTC ACTGGAAACA 
ACTTCACAAG AAGTGCATTC CAGCAAGAAG GATATTTCCA GTGACAGTGG GGTTGATCAG 
CCACAAAAGA TGCCAAACAT TCTCCCTGCA ACTCCTCAAT GTCATACAGC AAGCGTGAGT 
GAGCCCTTGC TATCTCAGGT AAACAATTCT CCATTGTCAA CACTGTTTCA TGGTAGTGAC 
ACTGCAAATG CCACACAAAA CTCTAATGAG AAAGGAGTGA AAACCCGTCT CCCTGTAGGA 
CTGAAATTAT TTGTCAAATC TCCTCAACTT CTCCGAAAGA GCTCCACCGT CCCAGGGAAG 
CAGGAAAAAG ATAGTATAAA TGCAGCTTCC AAAAGCCACG TGAGTACAAG TAAGCACAAG 
CAAAATGAAC GTGTGGCCAC CAAAGGTGCA ATAGATGCTG AATTAAAGGA CTCTAAGTCT 
GATACCGAAA TAAAAGATAA TTTTTCTTTG AGACTTAGTG TGGATACAGC ATCACCTCGT 
TCACATGAAA ATGGCAGCTT GGTGGTAGAC AGAGTAGATG AATTAGAAAG CAAACTAGTT 
AAGAGATCTT TTTCTTCTAG CAACAAGTCA CACTTGAAAC CAGCTCTAGG CATGAATGGA 
GCGAAAGCTC GAAGTCAGAG TTTCAGCGTT CATACAGGAG AAAAGTCATC TACCCTTGTG 
ACAGAAGGTC TTGGAAAAGT ACGGACTCAG ATTATTACAA ACACTTCTGA CAGAGGAAAT 
TCTCTAACAA GGCAGAACTC AGCCATGGAA GGACTTCAAA TCAAGGCCAT TCCTGGGTCA 
GGAGCAACAC AAGAGACTCT TTCAAATACT TCAAAAAACA CAGAAAATGC TTACTCTAGA 
CAAGGTTCTC TTGGAAGTAA TAGCAATAAC CAGCATGGAA GCCCAAGCAA ACTGCCATTC 
AGATCATCTC CAAAAGTAGA CTTATTTCGA AACACTTCAA AATATGATGG AAACAAATCA 
TTTTCTATGA AAGATGCACG GAGTCTATCT GTCCAGAGTG AGAAAAGCAG TGAAAATTTA 
AAACACGAAG CTCTAAATAA AAAGAGTGTT TTGCCAGCAG AATTGGAGTT AGATACATCA 
GCTACTGTAT CTTCCAAACA GATTTCATCA ATTCATAGCT TGGGTGGAAT AAAATGTCCG 
CATAAACTGG AAGCAATAAA ATCACAGAGA CAGCAGATGT CTTTAAAGTT AGCAGAAGCC 
CCTCAGGTTA CTGATGTTTT TAGTTCACCT GCTCTGCAAC CTACAATAGA GGAGAAGGTC 
ATGTTGTGCA TCCAAGAAAA TGTCCAGAAG GGTCAGGAAC AAAACAAATC TCCCACTGTA 
GAGACTAAAC AAAAGACTGG GCCCTCTATA GCTAGTTGGT TTGGCTTTCG AAAGAGTAAG 
CTGCCAGCAC TGAGCAGCAG GAAACCTGAC GTTCCTAAAA CAAAGGTAGA AAAAAAAGAA 
CCAAGAGTTT CAGGATTTGG AATTAAGCAG TCGAAATTAG AGAGAAGAAA AGAAAAAAAG 
CAAACTGAAC AACACTGTGA AGTAGAAAAT GAAATTAACA GGAAAACGGA CAGCAGTGAC 
TTTTTAGATG ATGCTATGGA GTTTAAAATT TTGAAGCCAT CACGAAACAC GTCTGGCAAG 
ATTGGATGTG AACAACTCAG AGGTTCCTCC ATAGCTACAT ATTCATCAAA AGACAGTTTT 
ATGAAGGAAC TTCTAAACAG AGTTGATAAG AAAGCAGCTC AGCAGACAGA AAGTGGATCA 
AATAATGTTT CCTACAGGAG TGTGTCGAAG GGCAGCTCCC AGGGCTCCTC TCTTCACAGC 
AACTCTATCA GCTCTCAAGG AAACCACAAG AAAAACAGCA ACACAAAAGC TGATATGGAA 
ATACAGAATC AGACCCTGGC TAACGTAGTC GCAGAAAACC TGCAAGAAGA AGAGGAGGAC 
ACCATGACAA GGACTACATG TCAGAGTCAT GTGATAGAGT CTTGCTGCCA GATGAGGACG 
TTGGACAGCG GGATTGGAAC CTTCCCACTC CCCGACTCAG GGAACCGATC TGCGGGGCGA 
CACGTGTCTA AGCAGGAATT AACCTTGGAA ACAGAAGCCC TAGCACCGTC TGAGCAAGTT 
CTTCTTTCAG CTCCTTCAGT AAAAGCCAAA ACTCTTGAGC GAGAAGTGCC TTCAACAGCT 
ACGAGTCAGG AGTCTGTGGA AAGCATGATT AGTCACTCAA CATCTGATCC AGCCATGACT 
GCCAAAGGTA TACGACCTTT TCAAAGTCGC CTTCCAAAAC CAGATTCCTC AGGAATAATT 
AACCTTGCAA AGCAAAGTGA ACAAGAACCA AGTTCTGTGA CCTCTACCTC ATTACAGCTT 
ACTGAAGAAA CTGTAGAAAA TAGAAAAGTT CTTTCAGATT GGACCACCAA GAAAACTACA 
AAAGCAAAGG ACAGAACTCT AAGAGTGTGC ACTTACTCTG CCAGCAGCAG TGACGCTGAA 
CCGGAGCCAG AGTATGGAAC AAATTATTTT CAAACTGCAG AGGAGATGTT AGTAGAGTTA 
ACAAAGAGCA ACAAACAAGC TGAGCAGGAA AAACAGAAGC AGAGAAAGTC AGTTTTGGGG 
AACCCGATGT CAATCTTGGA TTTATACCAG CACAGTCTCT ATGGCCATTT TGGAGAGGAT 
GGACCTGAAC AATTAACACA TTTCAGTTTA ATTGAGCAGC TGAGTGAACC TTCCGGTAAA 
GACAGCAAGG ACAAAGAGAG CCCAAGTAAC CTGAAGCAAA CTGAAGAAAC AAAAGAAGAT 
CCTCAGACTA GATTATCTAA AATTTCCCTG GAGTCTCTCA ATAAATTTAA CAGCAATAAC 
GTCCTTTTAT TAGAAAAGGA GAAGAACTGT CTGAACAAAG CTGAAGGACA AAAGGAAGAA 
AGCGGAAAGA AGGAAGCATC TTCCTTAAAC AGCTCCGATA GGCAGGACAT GGACAATTTG 
GAGTCCCTGA GCGATTCTTT GTACGACAGC TTCTCCTCTT GTGCCAGCCA AGGCTCTAAC 
GACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_015289933.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145419.1 Gallus gallus NCK-associated protein 5 (NCKAP5), transcript variant X7, mRNA.

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CloneID	OGa38640
Clone ID Related Accession (Same CDS sequence)	XM_015289932.1 , XM_015289933.1 , XM_015289932.2 , XM_015289933.2
Accession Version	XM_015289933.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	nck-associated protein 5 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAA AGAGACTACT ACAAAAAAGG GAAATTGGGA AAAGACTTTC TCTGGACAGC 
AGCCTTGTGG ACTATATGGA CTCAAACAAA TACATAGAGC ATCTAGTGAC ACAGCTAGAG 
GAACAGCGCT GGAATTTATG GAGGGAGAAG CTGTCTGTGG CTCGGCTGCA GCGAGAAGTT 
GCGCAGAGTA AGAGCGAAGG AACCATGCGC GAGAAACTGA TACATGAGTT GGAGGAAGAA 
AGACATTTAA GACTTGAGAG TGAAAAACGA TTACGGGAGG TAACACTGGA GTCAGAGCGT 
AACAGAGCTC AGATGCGTGG CCTACAAGAG CAGTTTTCCA GAATGGAGGA AACGGTGAGA 
AATCTACTAC AGAGCCAAGG ATCCCCAGGC CAGAATGGAG AAGGAACTGT CAATATCATG 
AAGGCATATC AGGAAAAGCT GTCAGAAGAC AGTAGGAAAT GCAAAGAAGT CATGGAAAAA 
AATCACACAG CAGTAGATGA AGATTCAAGA AATGAAAGCA ACAGCACTGA AGAGGAAAAA 
GAAAAGACAA AATTGCTACT GGAGCGTTTG AAAGCTCTGG AGGCAGAGAA TTCAGCACTG 
GCTCTGGAAA ATGAAAATCA GAGGGAACAG TATGAAAGAT GTCTTGATGA GGTAGCTAAT 
CAGGTCGTCC AGGCTCTACT TACTCAAAAG GATCTGAGGG AGGAATGTAT AAAGCTGAAA 
ACGAGAGTTT TTGATTTGGA GCAACAGAAT CAAACATTAA GTGTGCTTTT CCAGCAGAGA 
GTCAAACCAG CTTCTGACCT CCTCCTTCAG AAACTCCATT CGCGCATCTT AGACCTCTCA 
TCTGGAGATT TGCTGTCAGA AGTAGATCGA AACAGAAGCT CGGTGCCGTC ACGACCTGCT 
GATGCTCAGA TTCACGAATG CCAGCAGAAT GTGAAATCCA CTATACCTGT CTTGAAATGC 
CACAGTCAAT TAAATGTGAC AGGTCCTAGC CGCCTGTATC CCCGGAGCAG CTGCAGTAGC 
AGTGAACTCT CCCTTTCAAG TGCTTGCAGT GAATATTCCA GTGGTTCCTC CTACACTTGG 
AATGATGGGA AGACGTGCAG CAAAAGGTCA TCCGTGAGCT GGGATAAAAG AATAAGCATT 
GGTTCTTCAC TCCCAAGCAA CCTCTCTAGT CCTGCAGATG ATCTGCCTCC AACTCGTATC 
AAGGAAAACC ATATCCTAGA AGGACTGAAG AAATTACAGA GGCAAAAAGT ATTACTTGAA 
TCACCTTCAG TAATACCGAA ATGGGGTTAC AAAGATTGCA TGAACTCCAA TGAAGGAATA 
TATTCCCCTG GAGTTAAATG TGGCAGCCAT CATGAGCAGA CTCATTGTAA GCCAATGGAA 
ATAGGAAGTA GTTGCATTGA ACATAACAAA ACTTTCTCTT ATGATTCAGA CTCACATGAT 
GATGCAGATG ATGATTCTTC ATCTTTATTA TTGCTGCAGG AAGTACCAAG CAAAGACTGC 
AGGATCTATT GTAACAAATT AACTCACAGT GTTTCTGACA GTCTCTTTCA CTGGGATCCT 
AATAGGAAAC ATTTGCCAGA AAGAAGTTCA TATTTTAATT CAAAGGAAAG ACCAGAAAAA 
TTGACCAGTT TTGTCAGTGG ATTTCAGGCA GAAGTAGAAT CGTGCACCAG AACAAAGCTG 
CCTGATTTAC AGTTAGATCA AAGCCATGCT AATATAGGCT GGAAGGACCT TAATTTTCAG 
CTTTCAGATA CTGATGACAA TGAAGTCTTG GATGAATTGC ATATAGAAAG CAGTGATGAG 
AAAAGTCCAT CAGATTTATT AGCAACTCCT TTTACTGAGA AGTACACAAA GAACTCTGAT 
GTGCTCATAG TCACAGAGAA TTCTCAGTTT ATATCTAGTG ACAAAGAGGA AAGACAGACA 
TCTGCTCACT CAGACATTAG GCCAAAGACA TGTAATTTCA TTAAACAACA AAAAGTCATA 
AAAAAGACTT CTTCTGAGGA GTGCATTACA GTGATATTTG ATGCTGAGGA CGGTGAGCCT 
ATTGAATTCA GCTCGCATCA AATTGGAGTT GTCACTGTAA CAAGAAATGA GATATCAGTC 
AACCAACCAG AAAGTAGGTC CAGTGCAGAA TATACTGAAC TTATACCTCA GGGAATAGCT 
AATTTGCAGG CAGGAACAAA TGCTAGAGAC TACAGTGTTT TGGAAGGACC TGAAGACAAA 
ACTAAAAAAC AGACTCTTGA AGATAATACA GGGAATAAAA ATATGGTTGT CACCACAACC 
TGCAGTGAAT CTTCACCCTG TGGAAGAATC ATACTGCAAA ATGCACCACG ACAAAAACTA 
GTGAAGCCAG TGTATGATGT GTCGTACAAG ACCAATTCAA GCTCCATTGT TCATGCAGGA 
GTCAATCAAA AGCCAATCTT GACTAAAATA CCCAGCAGAG GTAAATTTTC TCCACAAAAA 
AACAAGATGA CAGAGAGTGA GGAGGCACCT GTAGCTACTT CAGTGACCCC TGCAACCCTT 
GAAAAATCAC CTGCTCTTCC ACCTGTAAAG CTTTCAAGAC TCAAAAAGGC ACAAAGTCCA 
CCTCGTAATT TTGACTCAAA AGTTGACTTC CAAGTTCCAA AGCCCCCTGC CCACCCCCTG 
CCCAGCTCAA AGCGTCCGAG CAGAGGCAAT GGGACAAAGT ATCCAAAGAG TCAGAGTCCA 
GCATCACCAC TGTTGCCTCA GCACCTTATA GAGCCTACTG ACTATGGAGA ACCTCCAACT 
AGAGACTCTA ATCATGATTT AGCAACTGTA GAAGCCAGGT CGCCATCCCC ACCCCTTCCT 
CCCGGCAGGT CAACGTCCCT ATTGATTCGT CCTGATTATG CTCATTCACA ACCTGCGGCT 
GTGAAGTTAG GAGGAGCTGT TCCAGGTGAA ACAGCAAAAA ATATACTGAA ACCATCACCA 
TTAAAAGGAA CTGCAAACTC TGGCATCCAT AATCAATCTA GTCATGATAT GCAAGCAAAA 
AGTCTATCTT CTGGGGCCAA AATTGAGTTA TCTTACCTTC AAGAAAATTC TGAAGCAATT 
ATGCAAAATA AATGTATATC TCAGCAACCC CATAGTGCAT GCCAAGGACT TTCCAAAGTT 
TCCACAAAGC AAGTCTGCCT GAAAAACACC AATCAGATGG GTCTCCACAG GAACCGTGAG 
TTTCTGAGCA TGGATTCTCA GACAGGCAGG TCCATTCCTC TGGGTCCGTC ACTGGAAACA 
ACTTCACAAG AAGTGCATTC CAGCAAGAAG GATATTTCCA GTGACAGTGG GGTTGATCAG 
CCACAAAAGA TGCCAAACAT TCTCCCTGCA ACTCCTCAAT GTCATACAGC AAGCGTGAGT 
GAGCCCTTGC TATCTCAGGT AAACAATTCT CCATTGTCAA CACTGTTTCA TGGTAGTGAC 
ACTGCAAATG CCACACAAAA CTCTAATGAG AAAGGAGTGA AAACCCGTCT CCCTGTAGGA 
CTGAAATTAT TTGTCAAATC TCCTCAACTT CTCCGAAAGA GCTCCACCGT CCCAGGGAAG 
CAGGAAAAAG ATAGTATAAA TGCAGCTTCC AAAAGCCACG TGAGTACAAG TAAGCACAAG 
CAAAATGAAC GTGTGGCCAC CAAAGGTGCA ATAGATGCTG AATTAAAGGA CTCTAAGTCT 
GATACCGAAA TAAAAGATAA TTTTTCTTTG AGACTTAGTG TGGATACAGC ATCACCTCGT 
TCACATGAAA ATGGCAGCTT GGTGGTAGAC AGAGTAGATG AATTAGAAAG CAAACTAGTT 
AAGAGATCTT TTTCTTCTAG CAACAAGTCA CACTTGAAAC CAGCTCTAGG CATGAATGGA 
GCGAAAGCTC GAAGTCAGAG TTTCAGCGTT CATACAGGAG AAAAGTCATC TACCCTTGTG 
ACAGAAGGTC TTGGAAAAGT ACGGACTCAG ATTATTACAA ACACTTCTGA CAGAGGAAAT 
TCTCTAACAA GGCAGAACTC AGCCATGGAA GGACTTCAAA TCAAGGCCAT TCCTGGGTCA 
GGAGCAACAC AAGAGACTCT TTCAAATACT TCAAAAAACA CAGAAAATGC TTACTCTAGA 
CAAGGTTCTC TTGGAAGTAA TAGCAATAAC CAGCATGGAA GCCCAAGCAA ACTGCCATTC 
AGATCATCTC CAAAAGTAGA CTTATTTCGA AACACTTCAA AATATGATGG AAACAAATCA 
TTTTCTATGA AAGATGCACG GAGTCTATCT GTCCAGAGTG AGAAAAGCAG TGAAAATTTA 
AAACACGAAG CTCTAAATAA AAAGAGTGTT TTGCCAGCAG AATTGGAGTT AGATACATCA 
GCTACTGTAT CTTCCAAACA GATTTCATCA ATTCATAGCT TGGGTGGAAT AAAATGTCCG 
CATAAACTGG AAGCAATAAA ATCACAGAGA CAGCAGATGT CTTTAAAGTT AGCAGAAGCC 
CCTCAGGTTA CTGATGTTTT TAGTTCACCT GCTCTGCAAC CTACAATAGA GGAGAAGGTC 
ATGTTGTGCA TCCAAGAAAA TGTCCAGAAG GGTCAGGAAC AAAACAAATC TCCCACTGTA 
GAGACTAAAC AAAAGACTGG GCCCTCTATA GCTAGTTGGT TTGGCTTTCG AAAGAGTAAG 
CTGCCAGCAC TGAGCAGCAG GAAACCTGAC GTTCCTAAAA CAAAGGTAGA AAAAAAAGAA 
CCAAGAGTTT CAGGATTTGG AATTAAGCAG TCGAAATTAG AGAGAAGAAA AGAAAAAAAG 
CAAACTGAAC AACACTGTGA AGTAGAAAAT GAAATTAACA GGAAAACGGA CAGCAGTGAC 
TTTTTAGATG ATGCTATGGA GTTTAAAATT TTGAAGCCAT CACGAAACAC GTCTGGCAAG 
ATTGGATGTG AACAACTCAG AGGTTCCTCC ATAGCTACAT ATTCATCAAA AGACAGTTTT 
ATGAAGGAAC TTCTAAACAG AGTTGATAAG AAAGCAGCTC AGCAGACAGA AAGTGGATCA 
AATAATGTTT CCTACAGGAG TGTGTCGAAG GGCAGCTCCC AGGGCTCCTC TCTTCACAGC 
AACTCTATCA GCTCTCAAGG AAACCACAAG AAAAACAGCA ACACAAAAGC TGATATGGAA 
ATACAGAATC AGACCCTGGC TAACGTAGTC GCAGAAAACC TGCAAGAAGA AGAGGAGGAC 
ACCATGACAA GGACTACATG TCAGAGTCAT GTGATAGAGT CTTGCTGCCA GATGAGGACG 
TTGGACAGCG GGATTGGAAC CTTCCCACTC CCCGACTCAG GGAACCGATC TGCGGGGCGA 
CACGTGTCTA AGCAGGAATT AACCTTGGAA ACAGAAGCCC TAGCACCGTC TGAGCAAGTT 
CTTCTTTCAG CTCCTTCAGT AAAAGCCAAA ACTCTTGAGC GAGAAGTGCC TTCAACAGCT 
ACGAGTCAGG AGTCTGTGGA AAGCATGATT AGTCACTCAA CATCTGATCC AGCCATGACT 
GCCAAAGGTA TACGACCTTT TCAAAGTCGC CTTCCAAAAC CAGATTCCTC AGGAATAATT 
AACCTTGCAA AGCAAAGTGA ACAAGAACCA AGTTCTGTGA CCTCTACCTC ATTACAGCTT 
ACTGAAGAAA CTGTAGAAAA TAGAAAAGTT CTTTCAGATT GGACCACCAA GAAAACTACA 
AAAGCAAAGG ACAGAACTCT AAGAGTGTGC ACTTACTCTG CCAGCAGCAG TGACGCTGAA 
CCGGAGCCAG AGTATGGAAC AAATTATTTT CAAACTGCAG AGGAGATGTT AGTAGAGTTA 
ACAAAGAGCA ACAAACAAGC TGAGCAGGAA AAACAGAAGC AGAGAAAGTC AGTTTTGGGG 
AACCCGATGT CAATCTTGGA TTTATACCAG CACAGTCTCT ATGGCCATTT TGGAGAGGAT 
GGACCTGAAC AATTAACACA TTTCAGTTTA ATTGAGCAGC TGAGTGAACC TTCCGGTAAA 
GACAGCAAGG ACAAAGAGAG CCCAAGTAAC CTGAAGCAAA CTGAAGAAAC AAAAGAAGAT 
CCTCAGACTA GATTATCTAA AATTTCCCTG GAGTCTCTCA ATAAATTTAA CAGCAATAAC 
GTCCTTTTAT TAGAAAAGGA GAAGAACTGT CTGAACAAAG CTGAAGGACA AAAGGAAGAA 
AGCGGAAAGA AGGAAGCATC TTCCTTAAAC AGCTCCGATA GGCAGGACAT GGACAATTTG 
GAGTCCCTGA GCGATTCTTT GTACGACAGC TTCTCCTCTT GTGCCAGCCA AGGCTCTAAC 
GACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145419.1
CDS	183..6431
Translation	MERKRLLQKREIGKRLSLDSSLVDYMDSNKYIEHLVTQLEEQRWNLWREKLSVARLQREVAQSKSEGTMREKLIHELEEERHLRLESEKRLREVTLESERNRAQMRGLQEQFSRMEETVRNLLQSQGSPGQNGEGTVNIMKAYQEKLSEDSRKCKEVMEKNHTAVDEDSRNESNSTEEEKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECIKLKTRVFDLEQQNQTLSVLFQQRVKPASDLLLQKLHSRILDLSSGDLLSEVDRNRSSVPSRPADAQIHECQQNVKSTIPVLKCHSQLNVTGPSRLYPRSSCSSSELSLSSACSEYSSGSSYTWNDGKTCSKRSSVSWDKRISIGSSLPSNLSSPADDLPPTRIKENHILEGLKKLQRQKVLLESPSVIPKWGYKDCMNSNEGIYSPGVKCGSHHEQTHCKPMEIGSSCIEHNKTFSYDSDSHDDADDDSSSLLLLQEVPSKDCRIYCNKLTHSVSDSLFHWDPNRKHLPERSSYFNSKERPEKLTSFVSGFQAEVESCTRTKLPDLQLDQSHANIGWKDLNFQLSDTDDNEVLDELHIESSDEKSPSDLLATPFTEKYTKNSDVLIVTENSQFISSDKEERQTSAHSDIRPKTCNFIKQQKVIKKTSSEECITVIFDAEDGEPIEFSSHQIGVVTVTRNEISVNQPESRSSAEYTELIPQGIANLQAGTNARDYSVLEGPEDKTKKQTLEDNTGNKNMVVTTTCSESSPCGRIILQNAPRQKLVKPVYDVSYKTNSSSIVHAGVNQKPILTKIPSRGKFSPQKNKMTESEEAPVATSVTPATLEKSPALPPVKLSRLKKAQSPPRNFDSKVDFQVPKPPAHPLPSSKRPSRGNGTKYPKSQSPASPLLPQHLIEPTDYGEPPTRDSNHDLATVEARSPSPPLPPGRSTSLLIRPDYAHSQPAAVKLGGAVPGETAKNILKPSPLKGTANSGIHNQSSHDMQAKSLSSGAKIELSYLQENSEAIMQNKCISQQPHSACQGLSKVSTKQVCLKNTNQMGLHRNREFLSMDSQTGRSIPLGPSLETTSQEVHSSKKDISSDSGVDQPQKMPNILPATPQCHTASVSEPLLSQVNNSPLSTLFHGSDTANATQNSNEKGVKTRLPVGLKLFVKSPQLLRKSSTVPGKQEKDSINAASKSHVSTSKHKQNERVATKGAIDAELKDSKSDTEIKDNFSLRLSVDTASPRSHENGSLVVDRVDELESKLVKRSFSSSNKSHLKPALGMNGAKARSQSFSVHTGEKSSTLVTEGLGKVRTQIITNTSDRGNSLTRQNSAMEGLQIKAIPGSGATQETLSNTSKNTENAYSRQGSLGSNSNNQHGSPSKLPFRSSPKVDLFRNTSKYDGNKSFSMKDARSLSVQSEKSSENLKHEALNKKSVLPAELELDTSATVSSKQISSIHSLGGIKCPHKLEAIKSQRQQMSLKLAEAPQVTDVFSSPALQPTIEEKVMLCIQENVQKGQEQNKSPTVETKQKTGPSIASWFGFRKSKLPALSSRKPDVPKTKVEKKEPRVSGFGIKQSKLERRKEKKQTEQHCEVENEINRKTDSSDFLDDAMEFKILKPSRNTSGKIGCEQLRGSSIATYSSKDSFMKELLNRVDKKAAQQTESGSNNVSYRSVSKGSSQGSSLHSNSISSQGNHKKNSNTKADMEIQNQTLANVVAENLQEEEEDTMTRTTCQSHVIESCCQMRTLDSGIGTFPLPDSGNRSAGRHVSKQELTLETEALAPSEQVLLSAPSVKAKTLEREVPSTATSQESVESMISHSTSDPAMTAKGIRPFQSRLPKPDSSGIINLAKQSEQEPSSVTSTSLQLTEETVENRKVLSDWTTKKTTKAKDRTLRVCTYSASSSDAEPEPEYGTNYFQTAEEMLVELTKSNKQAEQEKQKQRKSVLGNPMSILDLYQHSLYGHFGEDGPEQLTHFSLIEQLSEPSGKDSKDKESPSNLKQTEETKEDPQTRLSKISLESLNKFNSNNVLLLEKEKNCLNKAEGQKEESGKKEASSLNSSDRQDMDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_015289933.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NCK-associated protein 5 (NCKAP5), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289933.1

ORF Insert Sequence:

ATGGAGAGAA AGAGACTACT ACAAAAAAGG GAAATTGGGA AAAGACTTTC TCTGGACAGC 
AGCCTTGTGG ACTATATGGA CTCAAACAAA TACATAGAGC ATCTAGTGAC ACAGCTAGAG 
GAACAGCGCT GGAATTTATG GAGGGAGAAG CTGTCTGTGG CTCGGCTGCA GCGAGAAGTT 
GCGCAGAGTA AGAGCGAAGG AACCATGCGC GAGAAACTGA TACATGAGTT GGAGGAAGAA 
AGACATTTAA GACTTGAGAG TGAAAAACGA TTACGGGAGG TAACACTGGA GTCAGAGCGT 
AACAGAGCTC AGATGCGTGG CCTACAAGAG CAGTTTTCCA GAATGGAGGA AACGGTGAGA 
AATCTACTAC AGAGCCAAGG ATCCCCAGGC CAGAATGGAG AAGGAACTGT CAATATCATG 
AAGGCATATC AGGAAAAGCT GTCAGAAGAC AGTAGGAAAT GCAAAGAAGT CATGGAAAAA 
AATCACACAG CAGTAGATGA AGATTCAAGA AATGAAAGCA ACAGCACTGA AGAGGAAAAA 
GAAAAGACAA AATTGCTACT GGAGCGTTTG AAAGCTCTGG AGGCAGAGAA TTCAGCACTG 
GCTCTGGAAA ATGAAAATCA GAGGGAACAG TATGAAAGAT GTCTTGATGA GGTAGCTAAT 
CAGGTCGTCC AGGCTCTACT TACTCAAAAG GATCTGAGGG AGGAATGTAT AAAGCTGAAA 
ACGAGAGTTT TTGATTTGGA GCAACAGAAT CAAACATTAA GTGTGCTTTT CCAGCAGAGA 
GTCAAACCAG CTTCTGACCT CCTCCTTCAG AAACTCCATT CGCGCATCTT AGACCTCTCA 
TCTGGAGATT TGCTGTCAGA AGTAGATCGA AACAGAAGCT CGGTGCCGTC ACGACCTGCT 
GATGCTCAGA TTCACGAATG CCAGCAGAAT GTGAAATCCA CTATACCTGT CTTGAAATGC 
CACAGTCAAT TAAATGTGAC AGGTCCTAGC CGCCTGTATC CCCGGAGCAG CTGCAGTAGC 
AGTGAACTCT CCCTTTCAAG TGCTTGCAGT GAATATTCCA GTGGTTCCTC CTACACTTGG 
AATGATGGGA AGACGTGCAG CAAAAGGTCA TCCGTGAGCT GGGATAAAAG AATAAGCATT 
GGTTCTTCAC TCCCAAGCAA CCTCTCTAGT CCTGCAGATG ATCTGCCTCC AACTCGTATC 
AAGGAAAACC ATATCCTAGA AGGACTGAAG AAATTACAGA GGCAAAAAGT ATTACTTGAA 
TCACCTTCAG TAATACCGAA ATGGGGTTAC AAAGATTGCA TGAACTCCAA TGAAGGAATA 
TATTCCCCTG GAGTTAAATG TGGCAGCCAT CATGAGCAGA CTCATTGTAA GCCAATGGAA 
ATAGGAAGTA GTTGCATTGA ACATAACAAA ACTTTCTCTT ATGATTCAGA CTCACATGAT 
GATGCAGATG ATGATTCTTC ATCTTTATTA TTGCTGCAGG AAGTACCAAG CAAAGACTGC 
AGGATCTATT GTAACAAATT AACTCACAGT GTTTCTGACA GTCTCTTTCA CTGGGATCCT 
AATAGGAAAC ATTTGCCAGA AAGAAGTTCA TATTTTAATT CAAAGGAAAG ACCAGAAAAA 
TTGACCAGTT TTGTCAGTGG ATTTCAGGCA GAAGTAGAAT CGTGCACCAG AACAAAGCTG 
CCTGATTTAC AGTTAGATCA AAGCCATGCT AATATAGGCT GGAAGGACCT TAATTTTCAG 
CTTTCAGATA CTGATGACAA TGAAGTCTTG GATGAATTGC ATATAGAAAG CAGTGATGAG 
AAAAGTCCAT CAGATTTATT AGCAACTCCT TTTACTGAGA AGTACACAAA GAACTCTGAT 
GTGCTCATAG TCACAGAGAA TTCTCAGTTT ATATCTAGTG ACAAAGAGGA AAGACAGACA 
TCTGCTCACT CAGACATTAG GCCAAAGACA TGTAATTTCA TTAAACAACA AAAAGTCATA 
AAAAAGACTT CTTCTGAGGA GTGCATTACA GTGATATTTG ATGCTGAGGA CGGTGAGCCT 
ATTGAATTCA GCTCGCATCA AATTGGAGTT GTCACTGTAA CAAGAAATGA GATATCAGTC 
AACCAACCAG AAAGTAGGTC CAGTGCAGAA TATACTGAAC TTATACCTCA GGGAATAGCT 
AATTTGCAGG CAGGAACAAA TGCTAGAGAC TACAGTGTTT TGGAAGGACC TGAAGACAAA 
ACTAAAAAAC AGACTCTTGA AGATAATACA GGGAATAAAA ATATGGTTGT CACCACAACC 
TGCAGTGAAT CTTCACCCTG TGGAAGAATC ATACTGCAAA ATGCACCACG ACAAAAACTA 
GTGAAGCCAG TGTATGATGT GTCGTACAAG ACCAATTCAA GCTCCATTGT TCATGCAGGA 
GTCAATCAAA AGCCAATCTT GACTAAAATA CCCAGCAGAG GTAAATTTTC TCCACAAAAA 
AACAAGATGA CAGAGAGTGA GGAGGCACCT GTAGCTACTT CAGTGACCCC TGCAACCCTT 
GAAAAATCAC CTGCTCTTCC ACCTGTAAAG CTTTCAAGAC TCAAAAAGGC ACAAAGTCCA 
CCTCGTAATT TTGACTCAAA AGTTGACTTC CAAGTTCCAA AGCCCCCTGC CCACCCCCTG 
CCCAGCTCAA AGCGTCCGAG CAGAGGCAAT GGGACAAAGT ATCCAAAGAG TCAGAGTCCA 
GCATCACCAC TGTTGCCTCA GCACCTTATA GAGCCTACTG ACTATGGAGA ACCTCCAACT 
AGAGACTCTA ATCATGATTT AGCAACTGTA GAAGCCAGGT CGCCATCCCC ACCCCTTCCT 
CCCGGCAGGT CAACGTCCCT ATTGATTCGT CCTGATTATG CTCATTCACA ACCTGCGGCT 
GTGAAGTTAG GAGGAGCTGT TCCAGGTGAA ACAGCAAAAA ATATACTGAA ACCATCACCA 
TTAAAAGGAA CTGCAAACTC TGGCATCCAT AATCAATCTA GTCATGATAT GCAAGCAAAA 
AGTCTATCTT CTGGGGCCAA AATTGAGTTA TCTTACCTTC AAGAAAATTC TGAAGCAATT 
ATGCAAAATA AATGTATATC TCAGCAACCC CATAGTGCAT GCCAAGGACT TTCCAAAGTT 
TCCACAAAGC AAGTCTGCCT GAAAAACACC AATCAGATGG GTCTCCACAG GAACCGTGAG 
TTTCTGAGCA TGGATTCTCA GACAGGCAGG TCCATTCCTC TGGGTCCGTC ACTGGAAACA 
ACTTCACAAG AAGTGCATTC CAGCAAGAAG GATATTTCCA GTGACAGTGG GGTTGATCAG 
CCACAAAAGA TGCCAAACAT TCTCCCTGCA ACTCCTCAAT GTCATACAGC AAGCGTGAGT 
GAGCCCTTGC TATCTCAGGT AAACAATTCT CCATTGTCAA CACTGTTTCA TGGTAGTGAC 
ACTGCAAATG CCACACAAAA CTCTAATGAG AAAGGAGTGA AAACCCGTCT CCCTGTAGGA 
CTGAAATTAT TTGTCAAATC TCCTCAACTT CTCCGAAAGA GCTCCACCGT CCCAGGGAAG 
CAGGAAAAAG ATAGTATAAA TGCAGCTTCC AAAAGCCACG TGAGTACAAG TAAGCACAAG 
CAAAATGAAC GTGTGGCCAC CAAAGGTGCA ATAGATGCTG AATTAAAGGA CTCTAAGTCT 
GATACCGAAA TAAAAGATAA TTTTTCTTTG AGACTTAGTG TGGATACAGC ATCACCTCGT 
TCACATGAAA ATGGCAGCTT GGTGGTAGAC AGAGTAGATG AATTAGAAAG CAAACTAGTT 
AAGAGATCTT TTTCTTCTAG CAACAAGTCA CACTTGAAAC CAGCTCTAGG CATGAATGGA 
GCGAAAGCTC GAAGTCAGAG TTTCAGCGTT CATACAGGAG AAAAGTCATC TACCCTTGTG 
ACAGAAGGTC TTGGAAAAGT ACGGACTCAG ATTATTACAA ACACTTCTGA CAGAGGAAAT 
TCTCTAACAA GGCAGAACTC AGCCATGGAA GGACTTCAAA TCAAGGCCAT TCCTGGGTCA 
GGAGCAACAC AAGAGACTCT TTCAAATACT TCAAAAAACA CAGAAAATGC TTACTCTAGA 
CAAGGTTCTC TTGGAAGTAA TAGCAATAAC CAGCATGGAA GCCCAAGCAA ACTGCCATTC 
AGATCATCTC CAAAAGTAGA CTTATTTCGA AACACTTCAA AATATGATGG AAACAAATCA 
TTTTCTATGA AAGATGCACG GAGTCTATCT GTCCAGAGTG AGAAAAGCAG TGAAAATTTA 
AAACACGAAG CTCTAAATAA AAAGAGTGTT TTGCCAGCAG AATTGGAGTT AGATACATCA 
GCTACTGTAT CTTCCAAACA GATTTCATCA ATTCATAGCT TGGGTGGAAT AAAATGTCCG 
CATAAACTGG AAGCAATAAA ATCACAGAGA CAGCAGATGT CTTTAAAGTT AGCAGAAGCC 
CCTCAGGTTA CTGATGTTTT TAGTTCACCT GCTCTGCAAC CTACAATAGA GGAGAAGGTC 
ATGTTGTGCA TCCAAGAAAA TGTCCAGAAG GGTCAGGAAC AAAACAAATC TCCCACTGTA 
GAGACTAAAC AAAAGACTGG GCCCTCTATA GCTAGTTGGT TTGGCTTTCG AAAGAGTAAG 
CTGCCAGCAC TGAGCAGCAG GAAACCTGAC GTTCCTAAAA CAAAGGTAGA AAAAAAAGAA 
CCAAGAGTTT CAGGATTTGG AATTAAGCAG TCGAAATTAG AGAGAAGAAA AGAAAAAAAG 
CAAACTGAAC AACACTGTGA AGTAGAAAAT GAAATTAACA GGAAAACGGA CAGCAGTGAC 
TTTTTAGATG ATGCTATGGA GTTTAAAATT TTGAAGCCAT CACGAAACAC GTCTGGCAAG 
ATTGGATGTG AACAACTCAG AGGTTCCTCC ATAGCTACAT ATTCATCAAA AGACAGTTTT 
ATGAAGGAAC TTCTAAACAG AGTTGATAAG AAAGCAGCTC AGCAGACAGA AAGTGGATCA 
AATAATGTTT CCTACAGGAG TGTGTCGAAG GGCAGCTCCC AGGGCTCCTC TCTTCACAGC 
AACTCTATCA GCTCTCAAGG AAACCACAAG AAAAACAGCA ACACAAAAGC TGATATGGAA 
ATACAGAATC AGACCCTGGC TAACGTAGTC GCAGAAAACC TGCAAGAAGA AGAGGAGGAC 
ACCATGACAA GGACTACATG TCAGAGTCAT GTGATAGAGT CTTGCTGCCA GATGAGGACG 
TTGGACAGCG GGATTGGAAC CTTCCCACTC CCCGACTCAG GGAACCGATC TGCGGGGCGA 
CACGTGTCTA AGCAGGAATT AACCTTGGAA ACAGAAGCCC TAGCACCGTC TGAGCAAGTT 
CTTCTTTCAG CTCCTTCAGT AAAAGCCAAA ACTCTTGAGC GAGAAGTGCC TTCAACAGCT 
ACGAGTCAGG AGTCTGTGGA AAGCATGATT AGTCACTCAA CATCTGATCC AGCCATGACT 
GCCAAAGGTA TACGACCTTT TCAAAGTCGC CTTCCAAAAC CAGATTCCTC AGGAATAATT 
AACCTTGCAA AGCAAAGTGA ACAAGAACCA AGTTCTGTGA CCTCTACCTC ATTACAGCTT 
ACTGAAGAAA CTGTAGAAAA TAGAAAAGTT CTTTCAGATT GGACCACCAA GAAAACTACA 
AAAGCAAAGG ACAGAACTCT AAGAGTGTGC ACTTACTCTG CCAGCAGCAG TGACGCTGAA 
CCGGAGCCAG AGTATGGAAC AAATTATTTT CAAACTGCAG AGGAGATGTT AGTAGAGTTA 
ACAAAGAGCA ACAAACAAGC TGAGCAGGAA AAACAGAAGC AGAGAAAGTC AGTTTTGGGG 
AACCCGATGT CAATCTTGGA TTTATACCAG CACAGTCTCT ATGGCCATTT TGGAGAGGAT 
GGACCTGAAC AATTAACACA TTTCAGTTTA ATTGAGCAGC TGAGTGAACC TTCCGGTAAA 
GACAGCAAGG ACAAAGAGAG CCCAAGTAAC CTGAAGCAAA CTGAAGAAAC AAAAGAAGAT 
CCTCAGACTA GATTATCTAA AATTTCCCTG GAGTCTCTCA ATAAATTTAA CAGCAATAAC 
GTCCTTTTAT TAGAAAAGGA GAAGAACTGT CTGAACAAAG CTGAAGGACA AAAGGAAGAA 
AGCGGAAAGA AGGAAGCATC TTCCTTAAAC AGCTCCGATA GGCAGGACAT GGACAATTTG 
GAGTCCCTGA GCGATTCTTT GTACGACAGC TTCTCCTCTT GTGCCAGCCA AGGCTCTAAC 
GACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_015289932.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145418.1 Gallus gallus NCK associated protein 5 (NCKAP5), transcript variant X1, mRNA.

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CloneID	OGa38640
Clone ID Related Accession (Same CDS sequence)	XM_015289932.1 , XM_015289933.1 , XM_015289932.2 , XM_015289933.2
Accession Version	XM_015289932.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	nck-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289932.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAA AGAGACTACT ACAAAAAAGG GAAATTGGGA AAAGACTTTC TCTGGACAGC 
AGCCTTGTGG ACTATATGGA CTCAAACAAA TACATAGAGC ATCTAGTGAC ACAGCTAGAG 
GAACAGCGCT GGAATTTATG GAGGGAGAAG CTGTCTGTGG CTCGGCTGCA GCGAGAAGTT 
GCGCAGAGTA AGAGCGAAGG AACCATGCGC GAGAAACTGA TACATGAGTT GGAGGAAGAA 
AGACATTTAA GACTTGAGAG TGAAAAACGA TTACGGGAGG TAACACTGGA GTCAGAGCGT 
AACAGAGCTC AGATGCGTGG CCTACAAGAG CAGTTTTCCA GAATGGAGGA AACGGTGAGA 
AATCTACTAC AGAGCCAAGG ATCCCCAGGC CAGAATGGAG AAGGAACTGT CAATATCATG 
AAGGCATATC AGGAAAAGCT GTCAGAAGAC AGTAGGAAAT GCAAAGAAGT CATGGAAAAA 
AATCACACAG CAGTAGATGA AGATTCAAGA AATGAAAGCA ACAGCACTGA AGAGGAAAAA 
GAAAAGACAA AATTGCTACT GGAGCGTTTG AAAGCTCTGG AGGCAGAGAA TTCAGCACTG 
GCTCTGGAAA ATGAAAATCA GAGGGAACAG TATGAAAGAT GTCTTGATGA GGTAGCTAAT 
CAGGTCGTCC AGGCTCTACT TACTCAAAAG GATCTGAGGG AGGAATGTAT AAAGCTGAAA 
ACGAGAGTTT TTGATTTGGA GCAACAGAAT CAAACATTAA GTGTGCTTTT CCAGCAGAGA 
GTCAAACCAG CTTCTGACCT CCTCCTTCAG AAACTCCATT CGCGCATCTT AGACCTCTCA 
TCTGGAGATT TGCTGTCAGA AGTAGATCGA AACAGAAGCT CGGTGCCGTC ACGACCTGCT 
GATGCTCAGA TTCACGAATG CCAGCAGAAT GTGAAATCCA CTATACCTGT CTTGAAATGC 
CACAGTCAAT TAAATGTGAC AGGTCCTAGC CGCCTGTATC CCCGGAGCAG CTGCAGTAGC 
AGTGAACTCT CCCTTTCAAG TGCTTGCAGT GAATATTCCA GTGGTTCCTC CTACACTTGG 
AATGATGGGA AGACGTGCAG CAAAAGGTCA TCCGTGAGCT GGGATAAAAG AATAAGCATT 
GGTTCTTCAC TCCCAAGCAA CCTCTCTAGT CCTGCAGATG ATCTGCCTCC AACTCGTATC 
AAGGAAAACC ATATCCTAGA AGGACTGAAG AAATTACAGA GGCAAAAAGT ATTACTTGAA 
TCACCTTCAG TAATACCGAA ATGGGGTTAC AAAGATTGCA TGAACTCCAA TGAAGGAATA 
TATTCCCCTG GAGTTAAATG TGGCAGCCAT CATGAGCAGA CTCATTGTAA GCCAATGGAA 
ATAGGAAGTA GTTGCATTGA ACATAACAAA ACTTTCTCTT ATGATTCAGA CTCACATGAT 
GATGCAGATG ATGATTCTTC ATCTTTATTA TTGCTGCAGG AAGTACCAAG CAAAGACTGC 
AGGATCTATT GTAACAAATT AACTCACAGT GTTTCTGACA GTCTCTTTCA CTGGGATCCT 
AATAGGAAAC ATTTGCCAGA AAGAAGTTCA TATTTTAATT CAAAGGAAAG ACCAGAAAAA 
TTGACCAGTT TTGTCAGTGG ATTTCAGGCA GAAGTAGAAT CGTGCACCAG AACAAAGCTG 
CCTGATTTAC AGTTAGATCA AAGCCATGCT AATATAGGCT GGAAGGACCT TAATTTTCAG 
CTTTCAGATA CTGATGACAA TGAAGTCTTG GATGAATTGC ATATAGAAAG CAGTGATGAG 
AAAAGTCCAT CAGATTTATT AGCAACTCCT TTTACTGAGA AGTACACAAA GAACTCTGAT 
GTGCTCATAG TCACAGAGAA TTCTCAGTTT ATATCTAGTG ACAAAGAGGA AAGACAGACA 
TCTGCTCACT CAGACATTAG GCCAAAGACA TGTAATTTCA TTAAACAACA AAAAGTCATA 
AAAAAGACTT CTTCTGAGGA GTGCATTACA GTGATATTTG ATGCTGAGGA CGGTGAGCCT 
ATTGAATTCA GCTCGCATCA AATTGGAGTT GTCACTGTAA CAAGAAATGA GATATCAGTC 
AACCAACCAG AAAGTAGGTC CAGTGCAGAA TATACTGAAC TTATACCTCA GGGAATAGCT 
AATTTGCAGG CAGGAACAAA TGCTAGAGAC TACAGTGTTT TGGAAGGACC TGAAGACAAA 
ACTAAAAAAC AGACTCTTGA AGATAATACA GGGAATAAAA ATATGGTTGT CACCACAACC 
TGCAGTGAAT CTTCACCCTG TGGAAGAATC ATACTGCAAA ATGCACCACG ACAAAAACTA 
GTGAAGCCAG TGTATGATGT GTCGTACAAG ACCAATTCAA GCTCCATTGT TCATGCAGGA 
GTCAATCAAA AGCCAATCTT GACTAAAATA CCCAGCAGAG GTAAATTTTC TCCACAAAAA 
AACAAGATGA CAGAGAGTGA GGAGGCACCT GTAGCTACTT CAGTGACCCC TGCAACCCTT 
GAAAAATCAC CTGCTCTTCC ACCTGTAAAG CTTTCAAGAC TCAAAAAGGC ACAAAGTCCA 
CCTCGTAATT TTGACTCAAA AGTTGACTTC CAAGTTCCAA AGCCCCCTGC CCACCCCCTG 
CCCAGCTCAA AGCGTCCGAG CAGAGGCAAT GGGACAAAGT ATCCAAAGAG TCAGAGTCCA 
GCATCACCAC TGTTGCCTCA GCACCTTATA GAGCCTACTG ACTATGGAGA ACCTCCAACT 
AGAGACTCTA ATCATGATTT AGCAACTGTA GAAGCCAGGT CGCCATCCCC ACCCCTTCCT 
CCCGGCAGGT CAACGTCCCT ATTGATTCGT CCTGATTATG CTCATTCACA ACCTGCGGCT 
GTGAAGTTAG GAGGAGCTGT TCCAGGTGAA ACAGCAAAAA ATATACTGAA ACCATCACCA 
TTAAAAGGAA CTGCAAACTC TGGCATCCAT AATCAATCTA GTCATGATAT GCAAGCAAAA 
AGTCTATCTT CTGGGGCCAA AATTGAGTTA TCTTACCTTC AAGAAAATTC TGAAGCAATT 
ATGCAAAATA AATGTATATC TCAGCAACCC CATAGTGCAT GCCAAGGACT TTCCAAAGTT 
TCCACAAAGC AAGTCTGCCT GAAAAACACC AATCAGATGG GTCTCCACAG GAACCGTGAG 
TTTCTGAGCA TGGATTCTCA GACAGGCAGG TCCATTCCTC TGGGTCCGTC ACTGGAAACA 
ACTTCACAAG AAGTGCATTC CAGCAAGAAG GATATTTCCA GTGACAGTGG GGTTGATCAG 
CCACAAAAGA TGCCAAACAT TCTCCCTGCA ACTCCTCAAT GTCATACAGC AAGCGTGAGT 
GAGCCCTTGC TATCTCAGGT AAACAATTCT CCATTGTCAA CACTGTTTCA TGGTAGTGAC 
ACTGCAAATG CCACACAAAA CTCTAATGAG AAAGGAGTGA AAACCCGTCT CCCTGTAGGA 
CTGAAATTAT TTGTCAAATC TCCTCAACTT CTCCGAAAGA GCTCCACCGT CCCAGGGAAG 
CAGGAAAAAG ATAGTATAAA TGCAGCTTCC AAAAGCCACG TGAGTACAAG TAAGCACAAG 
CAAAATGAAC GTGTGGCCAC CAAAGGTGCA ATAGATGCTG AATTAAAGGA CTCTAAGTCT 
GATACCGAAA TAAAAGATAA TTTTTCTTTG AGACTTAGTG TGGATACAGC ATCACCTCGT 
TCACATGAAA ATGGCAGCTT GGTGGTAGAC AGAGTAGATG AATTAGAAAG CAAACTAGTT 
AAGAGATCTT TTTCTTCTAG CAACAAGTCA CACTTGAAAC CAGCTCTAGG CATGAATGGA 
GCGAAAGCTC GAAGTCAGAG TTTCAGCGTT CATACAGGAG AAAAGTCATC TACCCTTGTG 
ACAGAAGGTC TTGGAAAAGT ACGGACTCAG ATTATTACAA ACACTTCTGA CAGAGGAAAT 
TCTCTAACAA GGCAGAACTC AGCCATGGAA GGACTTCAAA TCAAGGCCAT TCCTGGGTCA 
GGAGCAACAC AAGAGACTCT TTCAAATACT TCAAAAAACA CAGAAAATGC TTACTCTAGA 
CAAGGTTCTC TTGGAAGTAA TAGCAATAAC CAGCATGGAA GCCCAAGCAA ACTGCCATTC 
AGATCATCTC CAAAAGTAGA CTTATTTCGA AACACTTCAA AATATGATGG AAACAAATCA 
TTTTCTATGA AAGATGCACG GAGTCTATCT GTCCAGAGTG AGAAAAGCAG TGAAAATTTA 
AAACACGAAG CTCTAAATAA AAAGAGTGTT TTGCCAGCAG AATTGGAGTT AGATACATCA 
GCTACTGTAT CTTCCAAACA GATTTCATCA ATTCATAGCT TGGGTGGAAT AAAATGTCCG 
CATAAACTGG AAGCAATAAA ATCACAGAGA CAGCAGATGT CTTTAAAGTT AGCAGAAGCC 
CCTCAGGTTA CTGATGTTTT TAGTTCACCT GCTCTGCAAC CTACAATAGA GGAGAAGGTC 
ATGTTGTGCA TCCAAGAAAA TGTCCAGAAG GGTCAGGAAC AAAACAAATC TCCCACTGTA 
GAGACTAAAC AAAAGACTGG GCCCTCTATA GCTAGTTGGT TTGGCTTTCG AAAGAGTAAG 
CTGCCAGCAC TGAGCAGCAG GAAACCTGAC GTTCCTAAAA CAAAGGTAGA AAAAAAAGAA 
CCAAGAGTTT CAGGATTTGG AATTAAGCAG TCGAAATTAG AGAGAAGAAA AGAAAAAAAG 
CAAACTGAAC AACACTGTGA AGTAGAAAAT GAAATTAACA GGAAAACGGA CAGCAGTGAC 
TTTTTAGATG ATGCTATGGA GTTTAAAATT TTGAAGCCAT CACGAAACAC GTCTGGCAAG 
ATTGGATGTG AACAACTCAG AGGTTCCTCC ATAGCTACAT ATTCATCAAA AGACAGTTTT 
ATGAAGGAAC TTCTAAACAG AGTTGATAAG AAAGCAGCTC AGCAGACAGA AAGTGGATCA 
AATAATGTTT CCTACAGGAG TGTGTCGAAG GGCAGCTCCC AGGGCTCCTC TCTTCACAGC 
AACTCTATCA GCTCTCAAGG AAACCACAAG AAAAACAGCA ACACAAAAGC TGATATGGAA 
ATACAGAATC AGACCCTGGC TAACGTAGTC GCAGAAAACC TGCAAGAAGA AGAGGAGGAC 
ACCATGACAA GGACTACATG TCAGAGTCAT GTGATAGAGT CTTGCTGCCA GATGAGGACG 
TTGGACAGCG GGATTGGAAC CTTCCCACTC CCCGACTCAG GGAACCGATC TGCGGGGCGA 
CACGTGTCTA AGCAGGAATT AACCTTGGAA ACAGAAGCCC TAGCACCGTC TGAGCAAGTT 
CTTCTTTCAG CTCCTTCAGT AAAAGCCAAA ACTCTTGAGC GAGAAGTGCC TTCAACAGCT 
ACGAGTCAGG AGTCTGTGGA AAGCATGATT AGTCACTCAA CATCTGATCC AGCCATGACT 
GCCAAAGGTA TACGACCTTT TCAAAGTCGC CTTCCAAAAC CAGATTCCTC AGGAATAATT 
AACCTTGCAA AGCAAAGTGA ACAAGAACCA AGTTCTGTGA CCTCTACCTC ATTACAGCTT 
ACTGAAGAAA CTGTAGAAAA TAGAAAAGTT CTTTCAGATT GGACCACCAA GAAAACTACA 
AAAGCAAAGG ACAGAACTCT AAGAGTGTGC ACTTACTCTG CCAGCAGCAG TGACGCTGAA 
CCGGAGCCAG AGTATGGAAC AAATTATTTT CAAACTGCAG AGGAGATGTT AGTAGAGTTA 
ACAAAGAGCA ACAAACAAGC TGAGCAGGAA AAACAGAAGC AGAGAAAGTC AGTTTTGGGG 
AACCCGATGT CAATCTTGGA TTTATACCAG CACAGTCTCT ATGGCCATTT TGGAGAGGAT 
GGACCTGAAC AATTAACACA TTTCAGTTTA ATTGAGCAGC TGAGTGAACC TTCCGGTAAA 
GACAGCAAGG ACAAAGAGAG CCCAAGTAAC CTGAAGCAAA CTGAAGAAAC AAAAGAAGAT 
CCTCAGACTA GATTATCTAA AATTTCCCTG GAGTCTCTCA ATAAATTTAA CAGCAATAAC 
GTCCTTTTAT TAGAAAAGGA GAAGAACTGT CTGAACAAAG CTGAAGGACA AAAGGAAGAA 
AGCGGAAAGA AGGAAGCATC TTCCTTAAAC AGCTCCGATA GGCAGGACAT GGACAATTTG 
GAGTCCCTGA GCGATTCTTT GTACGACAGC TTCTCCTCTT GTGCCAGCCA AGGCTCTAAC 
GACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145418.1
CDS	286..6534
Translation	MERKRLLQKREIGKRLSLDSSLVDYMDSNKYIEHLVTQLEEQRWNLWREKLSVARLQREVAQSKSEGTMREKLIHELEEERHLRLESEKRLREVTLESERNRAQMRGLQEQFSRMEETVRNLLQSQGSPGQNGEGTVNIMKAYQEKLSEDSRKCKEVMEKNHTAVDEDSRNESNSTEEEKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECIKLKTRVFDLEQQNQTLSVLFQQRVKPASDLLLQKLHSRILDLSSGDLLSEVDRNRSSVPSRPADAQIHECQQNVKSTIPVLKCHSQLNVTGPSRLYPRSSCSSSELSLSSACSEYSSGSSYTWNDGKTCSKRSSVSWDKRISIGSSLPSNLSSPADDLPPTRIKENHILEGLKKLQRQKVLLESPSVIPKWGYKDCMNSNEGIYSPGVKCGSHHEQTHCKPMEIGSSCIEHNKTFSYDSDSHDDADDDSSSLLLLQEVPSKDCRIYCNKLTHSVSDSLFHWDPNRKHLPERSSYFNSKERPEKLTSFVSGFQAEVESCTRTKLPDLQLDQSHANIGWKDLNFQLSDTDDNEVLDELHIESSDEKSPSDLLATPFTEKYTKNSDVLIVTENSQFISSDKEERQTSAHSDIRPKTCNFIKQQKVIKKTSSEECITVIFDAEDGEPIEFSSHQIGVVTVTRNEISVNQPESRSSAEYTELIPQGIANLQAGTNARDYSVLEGPEDKTKKQTLEDNTGNKNMVVTTTCSESSPCGRIILQNAPRQKLVKPVYDVSYKTNSSSIVHAGVNQKPILTKIPSRGKFSPQKNKMTESEEAPVATSVTPATLEKSPALPPVKLSRLKKAQSPPRNFDSKVDFQVPKPPAHPLPSSKRPSRGNGTKYPKSQSPASPLLPQHLIEPTDYGEPPTRDSNHDLATVEARSPSPPLPPGRSTSLLIRPDYAHSQPAAVKLGGAVPGETAKNILKPSPLKGTANSGIHNQSSHDMQAKSLSSGAKIELSYLQENSEAIMQNKCISQQPHSACQGLSKVSTKQVCLKNTNQMGLHRNREFLSMDSQTGRSIPLGPSLETTSQEVHSSKKDISSDSGVDQPQKMPNILPATPQCHTASVSEPLLSQVNNSPLSTLFHGSDTANATQNSNEKGVKTRLPVGLKLFVKSPQLLRKSSTVPGKQEKDSINAASKSHVSTSKHKQNERVATKGAIDAELKDSKSDTEIKDNFSLRLSVDTASPRSHENGSLVVDRVDELESKLVKRSFSSSNKSHLKPALGMNGAKARSQSFSVHTGEKSSTLVTEGLGKVRTQIITNTSDRGNSLTRQNSAMEGLQIKAIPGSGATQETLSNTSKNTENAYSRQGSLGSNSNNQHGSPSKLPFRSSPKVDLFRNTSKYDGNKSFSMKDARSLSVQSEKSSENLKHEALNKKSVLPAELELDTSATVSSKQISSIHSLGGIKCPHKLEAIKSQRQQMSLKLAEAPQVTDVFSSPALQPTIEEKVMLCIQENVQKGQEQNKSPTVETKQKTGPSIASWFGFRKSKLPALSSRKPDVPKTKVEKKEPRVSGFGIKQSKLERRKEKKQTEQHCEVENEINRKTDSSDFLDDAMEFKILKPSRNTSGKIGCEQLRGSSIATYSSKDSFMKELLNRVDKKAAQQTESGSNNVSYRSVSKGSSQGSSLHSNSISSQGNHKKNSNTKADMEIQNQTLANVVAENLQEEEEDTMTRTTCQSHVIESCCQMRTLDSGIGTFPLPDSGNRSAGRHVSKQELTLETEALAPSEQVLLSAPSVKAKTLEREVPSTATSQESVESMISHSTSDPAMTAKGIRPFQSRLPKPDSSGIINLAKQSEQEPSSVTSTSLQLTEETVENRKVLSDWTTKKTTKAKDRTLRVCTYSASSSDAEPEPEYGTNYFQTAEEMLVELTKSNKQAEQEKQKQRKSVLGNPMSILDLYQHSLYGHFGEDGPEQLTHFSLIEQLSEPSGKDSKDKESPSNLKQTEETKEDPQTRLSKISLESLNKFNSNNVLLLEKEKNCLNKAEGQKEESGKKEASSLNSSDRQDMDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_015289932.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NCK associated protein 5 (NCKAP5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289932.2

ORF Insert Sequence:

ATGGAGAGAA AGAGACTACT ACAAAAAAGG GAAATTGGGA AAAGACTTTC TCTGGACAGC 
AGCCTTGTGG ACTATATGGA CTCAAACAAA TACATAGAGC ATCTAGTGAC ACAGCTAGAG 
GAACAGCGCT GGAATTTATG GAGGGAGAAG CTGTCTGTGG CTCGGCTGCA GCGAGAAGTT 
GCGCAGAGTA AGAGCGAAGG AACCATGCGC GAGAAACTGA TACATGAGTT GGAGGAAGAA 
AGACATTTAA GACTTGAGAG TGAAAAACGA TTACGGGAGG TAACACTGGA GTCAGAGCGT 
AACAGAGCTC AGATGCGTGG CCTACAAGAG CAGTTTTCCA GAATGGAGGA AACGGTGAGA 
AATCTACTAC AGAGCCAAGG ATCCCCAGGC CAGAATGGAG AAGGAACTGT CAATATCATG 
AAGGCATATC AGGAAAAGCT GTCAGAAGAC AGTAGGAAAT GCAAAGAAGT CATGGAAAAA 
AATCACACAG CAGTAGATGA AGATTCAAGA AATGAAAGCA ACAGCACTGA AGAGGAAAAA 
GAAAAGACAA AATTGCTACT GGAGCGTTTG AAAGCTCTGG AGGCAGAGAA TTCAGCACTG 
GCTCTGGAAA ATGAAAATCA GAGGGAACAG TATGAAAGAT GTCTTGATGA GGTAGCTAAT 
CAGGTCGTCC AGGCTCTACT TACTCAAAAG GATCTGAGGG AGGAATGTAT AAAGCTGAAA 
ACGAGAGTTT TTGATTTGGA GCAACAGAAT CAAACATTAA GTGTGCTTTT CCAGCAGAGA 
GTCAAACCAG CTTCTGACCT CCTCCTTCAG AAACTCCATT CGCGCATCTT AGACCTCTCA 
TCTGGAGATT TGCTGTCAGA AGTAGATCGA AACAGAAGCT CGGTGCCGTC ACGACCTGCT 
GATGCTCAGA TTCACGAATG CCAGCAGAAT GTGAAATCCA CTATACCTGT CTTGAAATGC 
CACAGTCAAT TAAATGTGAC AGGTCCTAGC CGCCTGTATC CCCGGAGCAG CTGCAGTAGC 
AGTGAACTCT CCCTTTCAAG TGCTTGCAGT GAATATTCCA GTGGTTCCTC CTACACTTGG 
AATGATGGGA AGACGTGCAG CAAAAGGTCA TCCGTGAGCT GGGATAAAAG AATAAGCATT 
GGTTCTTCAC TCCCAAGCAA CCTCTCTAGT CCTGCAGATG ATCTGCCTCC AACTCGTATC 
AAGGAAAACC ATATCCTAGA AGGACTGAAG AAATTACAGA GGCAAAAAGT ATTACTTGAA 
TCACCTTCAG TAATACCGAA ATGGGGTTAC AAAGATTGCA TGAACTCCAA TGAAGGAATA 
TATTCCCCTG GAGTTAAATG TGGCAGCCAT CATGAGCAGA CTCATTGTAA GCCAATGGAA 
ATAGGAAGTA GTTGCATTGA ACATAACAAA ACTTTCTCTT ATGATTCAGA CTCACATGAT 
GATGCAGATG ATGATTCTTC ATCTTTATTA TTGCTGCAGG AAGTACCAAG CAAAGACTGC 
AGGATCTATT GTAACAAATT AACTCACAGT GTTTCTGACA GTCTCTTTCA CTGGGATCCT 
AATAGGAAAC ATTTGCCAGA AAGAAGTTCA TATTTTAATT CAAAGGAAAG ACCAGAAAAA 
TTGACCAGTT TTGTCAGTGG ATTTCAGGCA GAAGTAGAAT CGTGCACCAG AACAAAGCTG 
CCTGATTTAC AGTTAGATCA AAGCCATGCT AATATAGGCT GGAAGGACCT TAATTTTCAG 
CTTTCAGATA CTGATGACAA TGAAGTCTTG GATGAATTGC ATATAGAAAG CAGTGATGAG 
AAAAGTCCAT CAGATTTATT AGCAACTCCT TTTACTGAGA AGTACACAAA GAACTCTGAT 
GTGCTCATAG TCACAGAGAA TTCTCAGTTT ATATCTAGTG ACAAAGAGGA AAGACAGACA 
TCTGCTCACT CAGACATTAG GCCAAAGACA TGTAATTTCA TTAAACAACA AAAAGTCATA 
AAAAAGACTT CTTCTGAGGA GTGCATTACA GTGATATTTG ATGCTGAGGA CGGTGAGCCT 
ATTGAATTCA GCTCGCATCA AATTGGAGTT GTCACTGTAA CAAGAAATGA GATATCAGTC 
AACCAACCAG AAAGTAGGTC CAGTGCAGAA TATACTGAAC TTATACCTCA GGGAATAGCT 
AATTTGCAGG CAGGAACAAA TGCTAGAGAC TACAGTGTTT TGGAAGGACC TGAAGACAAA 
ACTAAAAAAC AGACTCTTGA AGATAATACA GGGAATAAAA ATATGGTTGT CACCACAACC 
TGCAGTGAAT CTTCACCCTG TGGAAGAATC ATACTGCAAA ATGCACCACG ACAAAAACTA 
GTGAAGCCAG TGTATGATGT GTCGTACAAG ACCAATTCAA GCTCCATTGT TCATGCAGGA 
GTCAATCAAA AGCCAATCTT GACTAAAATA CCCAGCAGAG GTAAATTTTC TCCACAAAAA 
AACAAGATGA CAGAGAGTGA GGAGGCACCT GTAGCTACTT CAGTGACCCC TGCAACCCTT 
GAAAAATCAC CTGCTCTTCC ACCTGTAAAG CTTTCAAGAC TCAAAAAGGC ACAAAGTCCA 
CCTCGTAATT TTGACTCAAA AGTTGACTTC CAAGTTCCAA AGCCCCCTGC CCACCCCCTG 
CCCAGCTCAA AGCGTCCGAG CAGAGGCAAT GGGACAAAGT ATCCAAAGAG TCAGAGTCCA 
GCATCACCAC TGTTGCCTCA GCACCTTATA GAGCCTACTG ACTATGGAGA ACCTCCAACT 
AGAGACTCTA ATCATGATTT AGCAACTGTA GAAGCCAGGT CGCCATCCCC ACCCCTTCCT 
CCCGGCAGGT CAACGTCCCT ATTGATTCGT CCTGATTATG CTCATTCACA ACCTGCGGCT 
GTGAAGTTAG GAGGAGCTGT TCCAGGTGAA ACAGCAAAAA ATATACTGAA ACCATCACCA 
TTAAAAGGAA CTGCAAACTC TGGCATCCAT AATCAATCTA GTCATGATAT GCAAGCAAAA 
AGTCTATCTT CTGGGGCCAA AATTGAGTTA TCTTACCTTC AAGAAAATTC TGAAGCAATT 
ATGCAAAATA AATGTATATC TCAGCAACCC CATAGTGCAT GCCAAGGACT TTCCAAAGTT 
TCCACAAAGC AAGTCTGCCT GAAAAACACC AATCAGATGG GTCTCCACAG GAACCGTGAG 
TTTCTGAGCA TGGATTCTCA GACAGGCAGG TCCATTCCTC TGGGTCCGTC ACTGGAAACA 
ACTTCACAAG AAGTGCATTC CAGCAAGAAG GATATTTCCA GTGACAGTGG GGTTGATCAG 
CCACAAAAGA TGCCAAACAT TCTCCCTGCA ACTCCTCAAT GTCATACAGC AAGCGTGAGT 
GAGCCCTTGC TATCTCAGGT AAACAATTCT CCATTGTCAA CACTGTTTCA TGGTAGTGAC 
ACTGCAAATG CCACACAAAA CTCTAATGAG AAAGGAGTGA AAACCCGTCT CCCTGTAGGA 
CTGAAATTAT TTGTCAAATC TCCTCAACTT CTCCGAAAGA GCTCCACCGT CCCAGGGAAG 
CAGGAAAAAG ATAGTATAAA TGCAGCTTCC AAAAGCCACG TGAGTACAAG TAAGCACAAG 
CAAAATGAAC GTGTGGCCAC CAAAGGTGCA ATAGATGCTG AATTAAAGGA CTCTAAGTCT 
GATACCGAAA TAAAAGATAA TTTTTCTTTG AGACTTAGTG TGGATACAGC ATCACCTCGT 
TCACATGAAA ATGGCAGCTT GGTGGTAGAC AGAGTAGATG AATTAGAAAG CAAACTAGTT 
AAGAGATCTT TTTCTTCTAG CAACAAGTCA CACTTGAAAC CAGCTCTAGG CATGAATGGA 
GCGAAAGCTC GAAGTCAGAG TTTCAGCGTT CATACAGGAG AAAAGTCATC TACCCTTGTG 
ACAGAAGGTC TTGGAAAAGT ACGGACTCAG ATTATTACAA ACACTTCTGA CAGAGGAAAT 
TCTCTAACAA GGCAGAACTC AGCCATGGAA GGACTTCAAA TCAAGGCCAT TCCTGGGTCA 
GGAGCAACAC AAGAGACTCT TTCAAATACT TCAAAAAACA CAGAAAATGC TTACTCTAGA 
CAAGGTTCTC TTGGAAGTAA TAGCAATAAC CAGCATGGAA GCCCAAGCAA ACTGCCATTC 
AGATCATCTC CAAAAGTAGA CTTATTTCGA AACACTTCAA AATATGATGG AAACAAATCA 
TTTTCTATGA AAGATGCACG GAGTCTATCT GTCCAGAGTG AGAAAAGCAG TGAAAATTTA 
AAACACGAAG CTCTAAATAA AAAGAGTGTT TTGCCAGCAG AATTGGAGTT AGATACATCA 
GCTACTGTAT CTTCCAAACA GATTTCATCA ATTCATAGCT TGGGTGGAAT AAAATGTCCG 
CATAAACTGG AAGCAATAAA ATCACAGAGA CAGCAGATGT CTTTAAAGTT AGCAGAAGCC 
CCTCAGGTTA CTGATGTTTT TAGTTCACCT GCTCTGCAAC CTACAATAGA GGAGAAGGTC 
ATGTTGTGCA TCCAAGAAAA TGTCCAGAAG GGTCAGGAAC AAAACAAATC TCCCACTGTA 
GAGACTAAAC AAAAGACTGG GCCCTCTATA GCTAGTTGGT TTGGCTTTCG AAAGAGTAAG 
CTGCCAGCAC TGAGCAGCAG GAAACCTGAC GTTCCTAAAA CAAAGGTAGA AAAAAAAGAA 
CCAAGAGTTT CAGGATTTGG AATTAAGCAG TCGAAATTAG AGAGAAGAAA AGAAAAAAAG 
CAAACTGAAC AACACTGTGA AGTAGAAAAT GAAATTAACA GGAAAACGGA CAGCAGTGAC 
TTTTTAGATG ATGCTATGGA GTTTAAAATT TTGAAGCCAT CACGAAACAC GTCTGGCAAG 
ATTGGATGTG AACAACTCAG AGGTTCCTCC ATAGCTACAT ATTCATCAAA AGACAGTTTT 
ATGAAGGAAC TTCTAAACAG AGTTGATAAG AAAGCAGCTC AGCAGACAGA AAGTGGATCA 
AATAATGTTT CCTACAGGAG TGTGTCGAAG GGCAGCTCCC AGGGCTCCTC TCTTCACAGC 
AACTCTATCA GCTCTCAAGG AAACCACAAG AAAAACAGCA ACACAAAAGC TGATATGGAA 
ATACAGAATC AGACCCTGGC TAACGTAGTC GCAGAAAACC TGCAAGAAGA AGAGGAGGAC 
ACCATGACAA GGACTACATG TCAGAGTCAT GTGATAGAGT CTTGCTGCCA GATGAGGACG 
TTGGACAGCG GGATTGGAAC CTTCCCACTC CCCGACTCAG GGAACCGATC TGCGGGGCGA 
CACGTGTCTA AGCAGGAATT AACCTTGGAA ACAGAAGCCC TAGCACCGTC TGAGCAAGTT 
CTTCTTTCAG CTCCTTCAGT AAAAGCCAAA ACTCTTGAGC GAGAAGTGCC TTCAACAGCT 
ACGAGTCAGG AGTCTGTGGA AAGCATGATT AGTCACTCAA CATCTGATCC AGCCATGACT 
GCCAAAGGTA TACGACCTTT TCAAAGTCGC CTTCCAAAAC CAGATTCCTC AGGAATAATT 
AACCTTGCAA AGCAAAGTGA ACAAGAACCA AGTTCTGTGA CCTCTACCTC ATTACAGCTT 
ACTGAAGAAA CTGTAGAAAA TAGAAAAGTT CTTTCAGATT GGACCACCAA GAAAACTACA 
AAAGCAAAGG ACAGAACTCT AAGAGTGTGC ACTTACTCTG CCAGCAGCAG TGACGCTGAA 
CCGGAGCCAG AGTATGGAAC AAATTATTTT CAAACTGCAG AGGAGATGTT AGTAGAGTTA 
ACAAAGAGCA ACAAACAAGC TGAGCAGGAA AAACAGAAGC AGAGAAAGTC AGTTTTGGGG 
AACCCGATGT CAATCTTGGA TTTATACCAG CACAGTCTCT ATGGCCATTT TGGAGAGGAT 
GGACCTGAAC AATTAACACA TTTCAGTTTA ATTGAGCAGC TGAGTGAACC TTCCGGTAAA 
GACAGCAAGG ACAAAGAGAG CCCAAGTAAC CTGAAGCAAA CTGAAGAAAC AAAAGAAGAT 
CCTCAGACTA GATTATCTAA AATTTCCCTG GAGTCTCTCA ATAAATTTAA CAGCAATAAC 
GTCCTTTTAT TAGAAAAGGA GAAGAACTGT CTGAACAAAG CTGAAGGACA AAAGGAAGAA 
AGCGGAAAGA AGGAAGCATC TTCCTTAAAC AGCTCCGATA GGCAGGACAT GGACAATTTG 
GAGTCCCTGA GCGATTCTTT GTACGACAGC TTCTCCTCTT GTGCCAGCCA AGGCTCTAAC 
GACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_015289933.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145419.1 Gallus gallus NCK associated protein 5 (NCKAP5), transcript variant X2, mRNA.

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CloneID	OGa38640
Clone ID Related Accession (Same CDS sequence)	XM_015289932.1 , XM_015289933.1 , XM_015289932.2 , XM_015289933.2
Accession Version	XM_015289933.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	nck-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289933.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAA AGAGACTACT ACAAAAAAGG GAAATTGGGA AAAGACTTTC TCTGGACAGC 
AGCCTTGTGG ACTATATGGA CTCAAACAAA TACATAGAGC ATCTAGTGAC ACAGCTAGAG 
GAACAGCGCT GGAATTTATG GAGGGAGAAG CTGTCTGTGG CTCGGCTGCA GCGAGAAGTT 
GCGCAGAGTA AGAGCGAAGG AACCATGCGC GAGAAACTGA TACATGAGTT GGAGGAAGAA 
AGACATTTAA GACTTGAGAG TGAAAAACGA TTACGGGAGG TAACACTGGA GTCAGAGCGT 
AACAGAGCTC AGATGCGTGG CCTACAAGAG CAGTTTTCCA GAATGGAGGA AACGGTGAGA 
AATCTACTAC AGAGCCAAGG ATCCCCAGGC CAGAATGGAG AAGGAACTGT CAATATCATG 
AAGGCATATC AGGAAAAGCT GTCAGAAGAC AGTAGGAAAT GCAAAGAAGT CATGGAAAAA 
AATCACACAG CAGTAGATGA AGATTCAAGA AATGAAAGCA ACAGCACTGA AGAGGAAAAA 
GAAAAGACAA AATTGCTACT GGAGCGTTTG AAAGCTCTGG AGGCAGAGAA TTCAGCACTG 
GCTCTGGAAA ATGAAAATCA GAGGGAACAG TATGAAAGAT GTCTTGATGA GGTAGCTAAT 
CAGGTCGTCC AGGCTCTACT TACTCAAAAG GATCTGAGGG AGGAATGTAT AAAGCTGAAA 
ACGAGAGTTT TTGATTTGGA GCAACAGAAT CAAACATTAA GTGTGCTTTT CCAGCAGAGA 
GTCAAACCAG CTTCTGACCT CCTCCTTCAG AAACTCCATT CGCGCATCTT AGACCTCTCA 
TCTGGAGATT TGCTGTCAGA AGTAGATCGA AACAGAAGCT CGGTGCCGTC ACGACCTGCT 
GATGCTCAGA TTCACGAATG CCAGCAGAAT GTGAAATCCA CTATACCTGT CTTGAAATGC 
CACAGTCAAT TAAATGTGAC AGGTCCTAGC CGCCTGTATC CCCGGAGCAG CTGCAGTAGC 
AGTGAACTCT CCCTTTCAAG TGCTTGCAGT GAATATTCCA GTGGTTCCTC CTACACTTGG 
AATGATGGGA AGACGTGCAG CAAAAGGTCA TCCGTGAGCT GGGATAAAAG AATAAGCATT 
GGTTCTTCAC TCCCAAGCAA CCTCTCTAGT CCTGCAGATG ATCTGCCTCC AACTCGTATC 
AAGGAAAACC ATATCCTAGA AGGACTGAAG AAATTACAGA GGCAAAAAGT ATTACTTGAA 
TCACCTTCAG TAATACCGAA ATGGGGTTAC AAAGATTGCA TGAACTCCAA TGAAGGAATA 
TATTCCCCTG GAGTTAAATG TGGCAGCCAT CATGAGCAGA CTCATTGTAA GCCAATGGAA 
ATAGGAAGTA GTTGCATTGA ACATAACAAA ACTTTCTCTT ATGATTCAGA CTCACATGAT 
GATGCAGATG ATGATTCTTC ATCTTTATTA TTGCTGCAGG AAGTACCAAG CAAAGACTGC 
AGGATCTATT GTAACAAATT AACTCACAGT GTTTCTGACA GTCTCTTTCA CTGGGATCCT 
AATAGGAAAC ATTTGCCAGA AAGAAGTTCA TATTTTAATT CAAAGGAAAG ACCAGAAAAA 
TTGACCAGTT TTGTCAGTGG ATTTCAGGCA GAAGTAGAAT CGTGCACCAG AACAAAGCTG 
CCTGATTTAC AGTTAGATCA AAGCCATGCT AATATAGGCT GGAAGGACCT TAATTTTCAG 
CTTTCAGATA CTGATGACAA TGAAGTCTTG GATGAATTGC ATATAGAAAG CAGTGATGAG 
AAAAGTCCAT CAGATTTATT AGCAACTCCT TTTACTGAGA AGTACACAAA GAACTCTGAT 
GTGCTCATAG TCACAGAGAA TTCTCAGTTT ATATCTAGTG ACAAAGAGGA AAGACAGACA 
TCTGCTCACT CAGACATTAG GCCAAAGACA TGTAATTTCA TTAAACAACA AAAAGTCATA 
AAAAAGACTT CTTCTGAGGA GTGCATTACA GTGATATTTG ATGCTGAGGA CGGTGAGCCT 
ATTGAATTCA GCTCGCATCA AATTGGAGTT GTCACTGTAA CAAGAAATGA GATATCAGTC 
AACCAACCAG AAAGTAGGTC CAGTGCAGAA TATACTGAAC TTATACCTCA GGGAATAGCT 
AATTTGCAGG CAGGAACAAA TGCTAGAGAC TACAGTGTTT TGGAAGGACC TGAAGACAAA 
ACTAAAAAAC AGACTCTTGA AGATAATACA GGGAATAAAA ATATGGTTGT CACCACAACC 
TGCAGTGAAT CTTCACCCTG TGGAAGAATC ATACTGCAAA ATGCACCACG ACAAAAACTA 
GTGAAGCCAG TGTATGATGT GTCGTACAAG ACCAATTCAA GCTCCATTGT TCATGCAGGA 
GTCAATCAAA AGCCAATCTT GACTAAAATA CCCAGCAGAG GTAAATTTTC TCCACAAAAA 
AACAAGATGA CAGAGAGTGA GGAGGCACCT GTAGCTACTT CAGTGACCCC TGCAACCCTT 
GAAAAATCAC CTGCTCTTCC ACCTGTAAAG CTTTCAAGAC TCAAAAAGGC ACAAAGTCCA 
CCTCGTAATT TTGACTCAAA AGTTGACTTC CAAGTTCCAA AGCCCCCTGC CCACCCCCTG 
CCCAGCTCAA AGCGTCCGAG CAGAGGCAAT GGGACAAAGT ATCCAAAGAG TCAGAGTCCA 
GCATCACCAC TGTTGCCTCA GCACCTTATA GAGCCTACTG ACTATGGAGA ACCTCCAACT 
AGAGACTCTA ATCATGATTT AGCAACTGTA GAAGCCAGGT CGCCATCCCC ACCCCTTCCT 
CCCGGCAGGT CAACGTCCCT ATTGATTCGT CCTGATTATG CTCATTCACA ACCTGCGGCT 
GTGAAGTTAG GAGGAGCTGT TCCAGGTGAA ACAGCAAAAA ATATACTGAA ACCATCACCA 
TTAAAAGGAA CTGCAAACTC TGGCATCCAT AATCAATCTA GTCATGATAT GCAAGCAAAA 
AGTCTATCTT CTGGGGCCAA AATTGAGTTA TCTTACCTTC AAGAAAATTC TGAAGCAATT 
ATGCAAAATA AATGTATATC TCAGCAACCC CATAGTGCAT GCCAAGGACT TTCCAAAGTT 
TCCACAAAGC AAGTCTGCCT GAAAAACACC AATCAGATGG GTCTCCACAG GAACCGTGAG 
TTTCTGAGCA TGGATTCTCA GACAGGCAGG TCCATTCCTC TGGGTCCGTC ACTGGAAACA 
ACTTCACAAG AAGTGCATTC CAGCAAGAAG GATATTTCCA GTGACAGTGG GGTTGATCAG 
CCACAAAAGA TGCCAAACAT TCTCCCTGCA ACTCCTCAAT GTCATACAGC AAGCGTGAGT 
GAGCCCTTGC TATCTCAGGT AAACAATTCT CCATTGTCAA CACTGTTTCA TGGTAGTGAC 
ACTGCAAATG CCACACAAAA CTCTAATGAG AAAGGAGTGA AAACCCGTCT CCCTGTAGGA 
CTGAAATTAT TTGTCAAATC TCCTCAACTT CTCCGAAAGA GCTCCACCGT CCCAGGGAAG 
CAGGAAAAAG ATAGTATAAA TGCAGCTTCC AAAAGCCACG TGAGTACAAG TAAGCACAAG 
CAAAATGAAC GTGTGGCCAC CAAAGGTGCA ATAGATGCTG AATTAAAGGA CTCTAAGTCT 
GATACCGAAA TAAAAGATAA TTTTTCTTTG AGACTTAGTG TGGATACAGC ATCACCTCGT 
TCACATGAAA ATGGCAGCTT GGTGGTAGAC AGAGTAGATG AATTAGAAAG CAAACTAGTT 
AAGAGATCTT TTTCTTCTAG CAACAAGTCA CACTTGAAAC CAGCTCTAGG CATGAATGGA 
GCGAAAGCTC GAAGTCAGAG TTTCAGCGTT CATACAGGAG AAAAGTCATC TACCCTTGTG 
ACAGAAGGTC TTGGAAAAGT ACGGACTCAG ATTATTACAA ACACTTCTGA CAGAGGAAAT 
TCTCTAACAA GGCAGAACTC AGCCATGGAA GGACTTCAAA TCAAGGCCAT TCCTGGGTCA 
GGAGCAACAC AAGAGACTCT TTCAAATACT TCAAAAAACA CAGAAAATGC TTACTCTAGA 
CAAGGTTCTC TTGGAAGTAA TAGCAATAAC CAGCATGGAA GCCCAAGCAA ACTGCCATTC 
AGATCATCTC CAAAAGTAGA CTTATTTCGA AACACTTCAA AATATGATGG AAACAAATCA 
TTTTCTATGA AAGATGCACG GAGTCTATCT GTCCAGAGTG AGAAAAGCAG TGAAAATTTA 
AAACACGAAG CTCTAAATAA AAAGAGTGTT TTGCCAGCAG AATTGGAGTT AGATACATCA 
GCTACTGTAT CTTCCAAACA GATTTCATCA ATTCATAGCT TGGGTGGAAT AAAATGTCCG 
CATAAACTGG AAGCAATAAA ATCACAGAGA CAGCAGATGT CTTTAAAGTT AGCAGAAGCC 
CCTCAGGTTA CTGATGTTTT TAGTTCACCT GCTCTGCAAC CTACAATAGA GGAGAAGGTC 
ATGTTGTGCA TCCAAGAAAA TGTCCAGAAG GGTCAGGAAC AAAACAAATC TCCCACTGTA 
GAGACTAAAC AAAAGACTGG GCCCTCTATA GCTAGTTGGT TTGGCTTTCG AAAGAGTAAG 
CTGCCAGCAC TGAGCAGCAG GAAACCTGAC GTTCCTAAAA CAAAGGTAGA AAAAAAAGAA 
CCAAGAGTTT CAGGATTTGG AATTAAGCAG TCGAAATTAG AGAGAAGAAA AGAAAAAAAG 
CAAACTGAAC AACACTGTGA AGTAGAAAAT GAAATTAACA GGAAAACGGA CAGCAGTGAC 
TTTTTAGATG ATGCTATGGA GTTTAAAATT TTGAAGCCAT CACGAAACAC GTCTGGCAAG 
ATTGGATGTG AACAACTCAG AGGTTCCTCC ATAGCTACAT ATTCATCAAA AGACAGTTTT 
ATGAAGGAAC TTCTAAACAG AGTTGATAAG AAAGCAGCTC AGCAGACAGA AAGTGGATCA 
AATAATGTTT CCTACAGGAG TGTGTCGAAG GGCAGCTCCC AGGGCTCCTC TCTTCACAGC 
AACTCTATCA GCTCTCAAGG AAACCACAAG AAAAACAGCA ACACAAAAGC TGATATGGAA 
ATACAGAATC AGACCCTGGC TAACGTAGTC GCAGAAAACC TGCAAGAAGA AGAGGAGGAC 
ACCATGACAA GGACTACATG TCAGAGTCAT GTGATAGAGT CTTGCTGCCA GATGAGGACG 
TTGGACAGCG GGATTGGAAC CTTCCCACTC CCCGACTCAG GGAACCGATC TGCGGGGCGA 
CACGTGTCTA AGCAGGAATT AACCTTGGAA ACAGAAGCCC TAGCACCGTC TGAGCAAGTT 
CTTCTTTCAG CTCCTTCAGT AAAAGCCAAA ACTCTTGAGC GAGAAGTGCC TTCAACAGCT 
ACGAGTCAGG AGTCTGTGGA AAGCATGATT AGTCACTCAA CATCTGATCC AGCCATGACT 
GCCAAAGGTA TACGACCTTT TCAAAGTCGC CTTCCAAAAC CAGATTCCTC AGGAATAATT 
AACCTTGCAA AGCAAAGTGA ACAAGAACCA AGTTCTGTGA CCTCTACCTC ATTACAGCTT 
ACTGAAGAAA CTGTAGAAAA TAGAAAAGTT CTTTCAGATT GGACCACCAA GAAAACTACA 
AAAGCAAAGG ACAGAACTCT AAGAGTGTGC ACTTACTCTG CCAGCAGCAG TGACGCTGAA 
CCGGAGCCAG AGTATGGAAC AAATTATTTT CAAACTGCAG AGGAGATGTT AGTAGAGTTA 
ACAAAGAGCA ACAAACAAGC TGAGCAGGAA AAACAGAAGC AGAGAAAGTC AGTTTTGGGG 
AACCCGATGT CAATCTTGGA TTTATACCAG CACAGTCTCT ATGGCCATTT TGGAGAGGAT 
GGACCTGAAC AATTAACACA TTTCAGTTTA ATTGAGCAGC TGAGTGAACC TTCCGGTAAA 
GACAGCAAGG ACAAAGAGAG CCCAAGTAAC CTGAAGCAAA CTGAAGAAAC AAAAGAAGAT 
CCTCAGACTA GATTATCTAA AATTTCCCTG GAGTCTCTCA ATAAATTTAA CAGCAATAAC 
GTCCTTTTAT TAGAAAAGGA GAAGAACTGT CTGAACAAAG CTGAAGGACA AAAGGAAGAA 
AGCGGAAAGA AGGAAGCATC TTCCTTAAAC AGCTCCGATA GGCAGGACAT GGACAATTTG 
GAGTCCCTGA GCGATTCTTT GTACGACAGC TTCTCCTCTT GTGCCAGCCA AGGCTCTAAC 
GACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145419.1
CDS	184..6432
Translation	MERKRLLQKREIGKRLSLDSSLVDYMDSNKYIEHLVTQLEEQRWNLWREKLSVARLQREVAQSKSEGTMREKLIHELEEERHLRLESEKRLREVTLESERNRAQMRGLQEQFSRMEETVRNLLQSQGSPGQNGEGTVNIMKAYQEKLSEDSRKCKEVMEKNHTAVDEDSRNESNSTEEEKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECIKLKTRVFDLEQQNQTLSVLFQQRVKPASDLLLQKLHSRILDLSSGDLLSEVDRNRSSVPSRPADAQIHECQQNVKSTIPVLKCHSQLNVTGPSRLYPRSSCSSSELSLSSACSEYSSGSSYTWNDGKTCSKRSSVSWDKRISIGSSLPSNLSSPADDLPPTRIKENHILEGLKKLQRQKVLLESPSVIPKWGYKDCMNSNEGIYSPGVKCGSHHEQTHCKPMEIGSSCIEHNKTFSYDSDSHDDADDDSSSLLLLQEVPSKDCRIYCNKLTHSVSDSLFHWDPNRKHLPERSSYFNSKERPEKLTSFVSGFQAEVESCTRTKLPDLQLDQSHANIGWKDLNFQLSDTDDNEVLDELHIESSDEKSPSDLLATPFTEKYTKNSDVLIVTENSQFISSDKEERQTSAHSDIRPKTCNFIKQQKVIKKTSSEECITVIFDAEDGEPIEFSSHQIGVVTVTRNEISVNQPESRSSAEYTELIPQGIANLQAGTNARDYSVLEGPEDKTKKQTLEDNTGNKNMVVTTTCSESSPCGRIILQNAPRQKLVKPVYDVSYKTNSSSIVHAGVNQKPILTKIPSRGKFSPQKNKMTESEEAPVATSVTPATLEKSPALPPVKLSRLKKAQSPPRNFDSKVDFQVPKPPAHPLPSSKRPSRGNGTKYPKSQSPASPLLPQHLIEPTDYGEPPTRDSNHDLATVEARSPSPPLPPGRSTSLLIRPDYAHSQPAAVKLGGAVPGETAKNILKPSPLKGTANSGIHNQSSHDMQAKSLSSGAKIELSYLQENSEAIMQNKCISQQPHSACQGLSKVSTKQVCLKNTNQMGLHRNREFLSMDSQTGRSIPLGPSLETTSQEVHSSKKDISSDSGVDQPQKMPNILPATPQCHTASVSEPLLSQVNNSPLSTLFHGSDTANATQNSNEKGVKTRLPVGLKLFVKSPQLLRKSSTVPGKQEKDSINAASKSHVSTSKHKQNERVATKGAIDAELKDSKSDTEIKDNFSLRLSVDTASPRSHENGSLVVDRVDELESKLVKRSFSSSNKSHLKPALGMNGAKARSQSFSVHTGEKSSTLVTEGLGKVRTQIITNTSDRGNSLTRQNSAMEGLQIKAIPGSGATQETLSNTSKNTENAYSRQGSLGSNSNNQHGSPSKLPFRSSPKVDLFRNTSKYDGNKSFSMKDARSLSVQSEKSSENLKHEALNKKSVLPAELELDTSATVSSKQISSIHSLGGIKCPHKLEAIKSQRQQMSLKLAEAPQVTDVFSSPALQPTIEEKVMLCIQENVQKGQEQNKSPTVETKQKTGPSIASWFGFRKSKLPALSSRKPDVPKTKVEKKEPRVSGFGIKQSKLERRKEKKQTEQHCEVENEINRKTDSSDFLDDAMEFKILKPSRNTSGKIGCEQLRGSSIATYSSKDSFMKELLNRVDKKAAQQTESGSNNVSYRSVSKGSSQGSSLHSNSISSQGNHKKNSNTKADMEIQNQTLANVVAENLQEEEEDTMTRTTCQSHVIESCCQMRTLDSGIGTFPLPDSGNRSAGRHVSKQELTLETEALAPSEQVLLSAPSVKAKTLEREVPSTATSQESVESMISHSTSDPAMTAKGIRPFQSRLPKPDSSGIINLAKQSEQEPSSVTSTSLQLTEETVENRKVLSDWTTKKTTKAKDRTLRVCTYSASSSDAEPEPEYGTNYFQTAEEMLVELTKSNKQAEQEKQKQRKSVLGNPMSILDLYQHSLYGHFGEDGPEQLTHFSLIEQLSEPSGKDSKDKESPSNLKQTEETKEDPQTRLSKISLESLNKFNSNNVLLLEKEKNCLNKAEGQKEESGKKEASSLNSSDRQDMDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_015289933.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NCK associated protein 5 (NCKAP5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289933.2

ORF Insert Sequence:

ATGGAGAGAA AGAGACTACT ACAAAAAAGG GAAATTGGGA AAAGACTTTC TCTGGACAGC 
AGCCTTGTGG ACTATATGGA CTCAAACAAA TACATAGAGC ATCTAGTGAC ACAGCTAGAG 
GAACAGCGCT GGAATTTATG GAGGGAGAAG CTGTCTGTGG CTCGGCTGCA GCGAGAAGTT 
GCGCAGAGTA AGAGCGAAGG AACCATGCGC GAGAAACTGA TACATGAGTT GGAGGAAGAA 
AGACATTTAA GACTTGAGAG TGAAAAACGA TTACGGGAGG TAACACTGGA GTCAGAGCGT 
AACAGAGCTC AGATGCGTGG CCTACAAGAG CAGTTTTCCA GAATGGAGGA AACGGTGAGA 
AATCTACTAC AGAGCCAAGG ATCCCCAGGC CAGAATGGAG AAGGAACTGT CAATATCATG 
AAGGCATATC AGGAAAAGCT GTCAGAAGAC AGTAGGAAAT GCAAAGAAGT CATGGAAAAA 
AATCACACAG CAGTAGATGA AGATTCAAGA AATGAAAGCA ACAGCACTGA AGAGGAAAAA 
GAAAAGACAA AATTGCTACT GGAGCGTTTG AAAGCTCTGG AGGCAGAGAA TTCAGCACTG 
GCTCTGGAAA ATGAAAATCA GAGGGAACAG TATGAAAGAT GTCTTGATGA GGTAGCTAAT 
CAGGTCGTCC AGGCTCTACT TACTCAAAAG GATCTGAGGG AGGAATGTAT AAAGCTGAAA 
ACGAGAGTTT TTGATTTGGA GCAACAGAAT CAAACATTAA GTGTGCTTTT CCAGCAGAGA 
GTCAAACCAG CTTCTGACCT CCTCCTTCAG AAACTCCATT CGCGCATCTT AGACCTCTCA 
TCTGGAGATT TGCTGTCAGA AGTAGATCGA AACAGAAGCT CGGTGCCGTC ACGACCTGCT 
GATGCTCAGA TTCACGAATG CCAGCAGAAT GTGAAATCCA CTATACCTGT CTTGAAATGC 
CACAGTCAAT TAAATGTGAC AGGTCCTAGC CGCCTGTATC CCCGGAGCAG CTGCAGTAGC 
AGTGAACTCT CCCTTTCAAG TGCTTGCAGT GAATATTCCA GTGGTTCCTC CTACACTTGG 
AATGATGGGA AGACGTGCAG CAAAAGGTCA TCCGTGAGCT GGGATAAAAG AATAAGCATT 
GGTTCTTCAC TCCCAAGCAA CCTCTCTAGT CCTGCAGATG ATCTGCCTCC AACTCGTATC 
AAGGAAAACC ATATCCTAGA AGGACTGAAG AAATTACAGA GGCAAAAAGT ATTACTTGAA 
TCACCTTCAG TAATACCGAA ATGGGGTTAC AAAGATTGCA TGAACTCCAA TGAAGGAATA 
TATTCCCCTG GAGTTAAATG TGGCAGCCAT CATGAGCAGA CTCATTGTAA GCCAATGGAA 
ATAGGAAGTA GTTGCATTGA ACATAACAAA ACTTTCTCTT ATGATTCAGA CTCACATGAT 
GATGCAGATG ATGATTCTTC ATCTTTATTA TTGCTGCAGG AAGTACCAAG CAAAGACTGC 
AGGATCTATT GTAACAAATT AACTCACAGT GTTTCTGACA GTCTCTTTCA CTGGGATCCT 
AATAGGAAAC ATTTGCCAGA AAGAAGTTCA TATTTTAATT CAAAGGAAAG ACCAGAAAAA 
TTGACCAGTT TTGTCAGTGG ATTTCAGGCA GAAGTAGAAT CGTGCACCAG AACAAAGCTG 
CCTGATTTAC AGTTAGATCA AAGCCATGCT AATATAGGCT GGAAGGACCT TAATTTTCAG 
CTTTCAGATA CTGATGACAA TGAAGTCTTG GATGAATTGC ATATAGAAAG CAGTGATGAG 
AAAAGTCCAT CAGATTTATT AGCAACTCCT TTTACTGAGA AGTACACAAA GAACTCTGAT 
GTGCTCATAG TCACAGAGAA TTCTCAGTTT ATATCTAGTG ACAAAGAGGA AAGACAGACA 
TCTGCTCACT CAGACATTAG GCCAAAGACA TGTAATTTCA TTAAACAACA AAAAGTCATA 
AAAAAGACTT CTTCTGAGGA GTGCATTACA GTGATATTTG ATGCTGAGGA CGGTGAGCCT 
ATTGAATTCA GCTCGCATCA AATTGGAGTT GTCACTGTAA CAAGAAATGA GATATCAGTC 
AACCAACCAG AAAGTAGGTC CAGTGCAGAA TATACTGAAC TTATACCTCA GGGAATAGCT 
AATTTGCAGG CAGGAACAAA TGCTAGAGAC TACAGTGTTT TGGAAGGACC TGAAGACAAA 
ACTAAAAAAC AGACTCTTGA AGATAATACA GGGAATAAAA ATATGGTTGT CACCACAACC 
TGCAGTGAAT CTTCACCCTG TGGAAGAATC ATACTGCAAA ATGCACCACG ACAAAAACTA 
GTGAAGCCAG TGTATGATGT GTCGTACAAG ACCAATTCAA GCTCCATTGT TCATGCAGGA 
GTCAATCAAA AGCCAATCTT GACTAAAATA CCCAGCAGAG GTAAATTTTC TCCACAAAAA 
AACAAGATGA CAGAGAGTGA GGAGGCACCT GTAGCTACTT CAGTGACCCC TGCAACCCTT 
GAAAAATCAC CTGCTCTTCC ACCTGTAAAG CTTTCAAGAC TCAAAAAGGC ACAAAGTCCA 
CCTCGTAATT TTGACTCAAA AGTTGACTTC CAAGTTCCAA AGCCCCCTGC CCACCCCCTG 
CCCAGCTCAA AGCGTCCGAG CAGAGGCAAT GGGACAAAGT ATCCAAAGAG TCAGAGTCCA 
GCATCACCAC TGTTGCCTCA GCACCTTATA GAGCCTACTG ACTATGGAGA ACCTCCAACT 
AGAGACTCTA ATCATGATTT AGCAACTGTA GAAGCCAGGT CGCCATCCCC ACCCCTTCCT 
CCCGGCAGGT CAACGTCCCT ATTGATTCGT CCTGATTATG CTCATTCACA ACCTGCGGCT 
GTGAAGTTAG GAGGAGCTGT TCCAGGTGAA ACAGCAAAAA ATATACTGAA ACCATCACCA 
TTAAAAGGAA CTGCAAACTC TGGCATCCAT AATCAATCTA GTCATGATAT GCAAGCAAAA 
AGTCTATCTT CTGGGGCCAA AATTGAGTTA TCTTACCTTC AAGAAAATTC TGAAGCAATT 
ATGCAAAATA AATGTATATC TCAGCAACCC CATAGTGCAT GCCAAGGACT TTCCAAAGTT 
TCCACAAAGC AAGTCTGCCT GAAAAACACC AATCAGATGG GTCTCCACAG GAACCGTGAG 
TTTCTGAGCA TGGATTCTCA GACAGGCAGG TCCATTCCTC TGGGTCCGTC ACTGGAAACA 
ACTTCACAAG AAGTGCATTC CAGCAAGAAG GATATTTCCA GTGACAGTGG GGTTGATCAG 
CCACAAAAGA TGCCAAACAT TCTCCCTGCA ACTCCTCAAT GTCATACAGC AAGCGTGAGT 
GAGCCCTTGC TATCTCAGGT AAACAATTCT CCATTGTCAA CACTGTTTCA TGGTAGTGAC 
ACTGCAAATG CCACACAAAA CTCTAATGAG AAAGGAGTGA AAACCCGTCT CCCTGTAGGA 
CTGAAATTAT TTGTCAAATC TCCTCAACTT CTCCGAAAGA GCTCCACCGT CCCAGGGAAG 
CAGGAAAAAG ATAGTATAAA TGCAGCTTCC AAAAGCCACG TGAGTACAAG TAAGCACAAG 
CAAAATGAAC GTGTGGCCAC CAAAGGTGCA ATAGATGCTG AATTAAAGGA CTCTAAGTCT 
GATACCGAAA TAAAAGATAA TTTTTCTTTG AGACTTAGTG TGGATACAGC ATCACCTCGT 
TCACATGAAA ATGGCAGCTT GGTGGTAGAC AGAGTAGATG AATTAGAAAG CAAACTAGTT 
AAGAGATCTT TTTCTTCTAG CAACAAGTCA CACTTGAAAC CAGCTCTAGG CATGAATGGA 
GCGAAAGCTC GAAGTCAGAG TTTCAGCGTT CATACAGGAG AAAAGTCATC TACCCTTGTG 
ACAGAAGGTC TTGGAAAAGT ACGGACTCAG ATTATTACAA ACACTTCTGA CAGAGGAAAT 
TCTCTAACAA GGCAGAACTC AGCCATGGAA GGACTTCAAA TCAAGGCCAT TCCTGGGTCA 
GGAGCAACAC AAGAGACTCT TTCAAATACT TCAAAAAACA CAGAAAATGC TTACTCTAGA 
CAAGGTTCTC TTGGAAGTAA TAGCAATAAC CAGCATGGAA GCCCAAGCAA ACTGCCATTC 
AGATCATCTC CAAAAGTAGA CTTATTTCGA AACACTTCAA AATATGATGG AAACAAATCA 
TTTTCTATGA AAGATGCACG GAGTCTATCT GTCCAGAGTG AGAAAAGCAG TGAAAATTTA 
AAACACGAAG CTCTAAATAA AAAGAGTGTT TTGCCAGCAG AATTGGAGTT AGATACATCA 
GCTACTGTAT CTTCCAAACA GATTTCATCA ATTCATAGCT TGGGTGGAAT AAAATGTCCG 
CATAAACTGG AAGCAATAAA ATCACAGAGA CAGCAGATGT CTTTAAAGTT AGCAGAAGCC 
CCTCAGGTTA CTGATGTTTT TAGTTCACCT GCTCTGCAAC CTACAATAGA GGAGAAGGTC 
ATGTTGTGCA TCCAAGAAAA TGTCCAGAAG GGTCAGGAAC AAAACAAATC TCCCACTGTA 
GAGACTAAAC AAAAGACTGG GCCCTCTATA GCTAGTTGGT TTGGCTTTCG AAAGAGTAAG 
CTGCCAGCAC TGAGCAGCAG GAAACCTGAC GTTCCTAAAA CAAAGGTAGA AAAAAAAGAA 
CCAAGAGTTT CAGGATTTGG AATTAAGCAG TCGAAATTAG AGAGAAGAAA AGAAAAAAAG 
CAAACTGAAC AACACTGTGA AGTAGAAAAT GAAATTAACA GGAAAACGGA CAGCAGTGAC 
TTTTTAGATG ATGCTATGGA GTTTAAAATT TTGAAGCCAT CACGAAACAC GTCTGGCAAG 
ATTGGATGTG AACAACTCAG AGGTTCCTCC ATAGCTACAT ATTCATCAAA AGACAGTTTT 
ATGAAGGAAC TTCTAAACAG AGTTGATAAG AAAGCAGCTC AGCAGACAGA AAGTGGATCA 
AATAATGTTT CCTACAGGAG TGTGTCGAAG GGCAGCTCCC AGGGCTCCTC TCTTCACAGC 
AACTCTATCA GCTCTCAAGG AAACCACAAG AAAAACAGCA ACACAAAAGC TGATATGGAA 
ATACAGAATC AGACCCTGGC TAACGTAGTC GCAGAAAACC TGCAAGAAGA AGAGGAGGAC 
ACCATGACAA GGACTACATG TCAGAGTCAT GTGATAGAGT CTTGCTGCCA GATGAGGACG 
TTGGACAGCG GGATTGGAAC CTTCCCACTC CCCGACTCAG GGAACCGATC TGCGGGGCGA 
CACGTGTCTA AGCAGGAATT AACCTTGGAA ACAGAAGCCC TAGCACCGTC TGAGCAAGTT 
CTTCTTTCAG CTCCTTCAGT AAAAGCCAAA ACTCTTGAGC GAGAAGTGCC TTCAACAGCT 
ACGAGTCAGG AGTCTGTGGA AAGCATGATT AGTCACTCAA CATCTGATCC AGCCATGACT 
GCCAAAGGTA TACGACCTTT TCAAAGTCGC CTTCCAAAAC CAGATTCCTC AGGAATAATT 
AACCTTGCAA AGCAAAGTGA ACAAGAACCA AGTTCTGTGA CCTCTACCTC ATTACAGCTT 
ACTGAAGAAA CTGTAGAAAA TAGAAAAGTT CTTTCAGATT GGACCACCAA GAAAACTACA 
AAAGCAAAGG ACAGAACTCT AAGAGTGTGC ACTTACTCTG CCAGCAGCAG TGACGCTGAA 
CCGGAGCCAG AGTATGGAAC AAATTATTTT CAAACTGCAG AGGAGATGTT AGTAGAGTTA 
ACAAAGAGCA ACAAACAAGC TGAGCAGGAA AAACAGAAGC AGAGAAAGTC AGTTTTGGGG 
AACCCGATGT CAATCTTGGA TTTATACCAG CACAGTCTCT ATGGCCATTT TGGAGAGGAT 
GGACCTGAAC AATTAACACA TTTCAGTTTA ATTGAGCAGC TGAGTGAACC TTCCGGTAAA 
GACAGCAAGG ACAAAGAGAG CCCAAGTAAC CTGAAGCAAA CTGAAGAAAC AAAAGAAGAT 
CCTCAGACTA GATTATCTAA AATTTCCCTG GAGTCTCTCA ATAAATTTAA CAGCAATAAC 
GTCCTTTTAT TAGAAAAGGA GAAGAACTGT CTGAACAAAG CTGAAGGACA AAAGGAAGAA 
AGCGGAAAGA AGGAAGCATC TTCCTTAAAC AGCTCCGATA GGCAGGACAT GGACAATTTG 
GAGTCCCTGA GCGATTCTTT GTACGACAGC TTCTCCTCTT GTGCCAGCCA AGGCTCTAAC 
GACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP5 ( XM_015289934.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015145420.1 Gallus gallus NCK associated protein 5 (NCKAP5), transcript variant X6, mRNA.

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CloneID	OGa38641
Clone ID Related Accession (Same CDS sequence)	XM_015289934.1
Accession Version	XM_015289934.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	nck-associated protein 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGAGA AACTGATACA TGAGTTGGAG GAAGAAAGAC ATTTAAGACT TGAGAGTGAA 
AAACGATTAC GGGAGGTAAC ACTGGAGTCA GAGCGTAACA GAGCTCAGAT GCGTGGCCTA 
CAAGAGCAGT TTTCCAGAAT GGAGGAAACG GTGAGAAATC TACTACAGAG CCAAGGATCC 
CCAGGCCAGA ATGGAGAAGG AACTGTCAAT ATCATGAAGG CATATCAGGA AAAGCTGTCA 
GAAGACAGTA GGAAATGCAA AGAAGTCATG GAAAAAAATC ACACAGCAGT AGATGAAGAT 
TCAAGAAATG AAAGCAACAG CACTGAAGAG GAAAAAGAAA AGACAAAATT GCTACTGGAG 
CGTTTGAAAG CTCTGGAGGC AGAGAATTCA GCACTGGCTC TGGAAAATGA AAATCAGAGG 
GAACAGTATG AAAGATGTCT TGATGAGGTA GCTAATCAGG TCGTCCAGGC TCTACTTACT 
CAAAAGGATC TGAGGGAGGA ATGTATAAAG CTGAAAACGA GAGTTTTTGA TTTGGAGCAA 
CAGAATCAAA CATTAAGTGT GCTTTTCCAG CAGAGAGTCA AACCAGCTTC TGACCTCCTC 
CTTCAGAAAC TCCATTCGCG CATCTTAGAC CTCTCATCTG GAGATTTGCT GTCAGAAGTA 
GATCGAAACA GAAGCTCGGT GCCGTCACGA CCTGCTGATG CTCAGATTCA CGAATGCCAG 
CAGAATGTGA AATCCACTAT ACCTGTCTTG AAATGCCACA GTCAATTAAA TGTGACAGGT 
CCTAGCCGCC TGTATCCCCG GAGCAGCTGC AGTAGCAGTG AACTCTCCCT TTCAAGTGCT 
TGCAGTGAAT ATTCCAGTGG TTCCTCCTAC ACTTGGAATG ATGGGAAGAC GTGCAGCAAA 
AGGTCATCCG TGAGCTGGGA TAAAAGAATA AGCATTGGTT CTTCACTCCC AAGCAACCTC 
TCTAGTCCTG CAGATGATCT GCCTCCAACT CGTATCAAGG AAAACCATAT CCTAGAAGGA 
CTGAAGAAAT TACAGAGGCA AAAAGTATTA CTTGAATCAC CTTCAGTAAT ACCGAAATGG 
GGTTACAAAG ATTGCATGAA CTCCAATGAA GGAATATATT CCCCTGGAGT TAAATGTGGC 
AGCCATCATG AGCAGACTCA TTGTAAGCCA ATGGAAATAG GAAGTAGTTG CATTGAACAT 
AACAAAACTT TCTCTTATGA TTCAGACTCA CATGATGATG CAGATGATGA TTCTTCATCT 
TTATTATTGC TGCAGGAAGT ACCAAGCAAA GACTGCAGGA TCTATTGTAA CAAATTAACT 
CACAGTGTTT CTGACAGTCT CTTTCACTGG GATCCTAATA GGAAACATTT GCCAGAAAGA 
AGTTCATATT TTAATTCAAA GGAAAGACCA GAAAAATTGA CCAGTTTTGT CAGTGGATTT 
CAGGCAGAAG TAGAATCGTG CACCAGAACA AAGCTGCCTG ATTTACAGTT AGATCAAAGC 
CATGCTAATA TAGGCTGGAA GGACCTTAAT TTTCAGCTTT CAGATACTGA TGACAATGAA 
GTCTTGGATG AATTGCATAT AGAAAGCAGT GATGAGAAAA GTCCATCAGA TTTATTAGCA 
ACTCCTTTTA CTGAGAAGTA CACAAAGAAC TCTGATGTGC TCATAGTCAC AGAGAATTCT 
CAGTTTATAT CTAGTGACAA AGAGGAAAGA CAGACATCTG CTCACTCAGA CATTAGGCCA 
AAGACATGTA ATTTCATTAA ACAACAAAAA GTCATAAAAA AGACTTCTTC TGAGGAGTGC 
ATTACAGTGA TATTTGATGC TGAGGACGGT GAGCCTATTG AATTCAGCTC GCATCAAATT 
GGAGTTGTCA CTGTAACAAG AAATGAGATA TCAGTCAACC AACCAGAAAG TAGGTCCAGT 
GCAGAATATA CTGAACTTAT ACCTCAGGGA ATAGCTAATT TGCAGGCAGG AACAAATGCT 
AGAGACTACA GTGTTTTGGA AGGACCTGAA GACAAAACTA AAAAACAGAC TCTTGAAGAT 
AATACAGGGA ATAAAAATAT GGTTGTCACC ACAACCTGCA GTGAATCTTC ACCCTGTGGA 
AGAATCATAC TGCAAAATGC ACCACGACAA AAACTAGTGA AGCCAGTGTA TGATGTGTCG 
TACAAGACCA ATTCAAGCTC CATTGTTCAT GCAGGAGTCA ATCAAAAGCC AATCTTGACT 
AAAATACCCA GCAGAGGTAA ATTTTCTCCA CAAAAAAACA AGATGACAGA GAGTGAGGAG 
GCACCTGTAG CTACTTCAGT GACCCCTGCA ACCCTTGAAA AATCACCTGC TCTTCCACCT 
GTAAAGCTTT CAAGACTCAA AAAGGCACAA AGTCCACCTC GTAATTTTGA CTCAAAAGTT 
GACTTCCAAG TTCCAAAGCC CCCTGCCCAC CCCCTGCCCA GCTCAAAGCG TCCGAGCAGA 
GGCAATGGGA CAAAGTATCC AAAGAGTCAG AGTCCAGCAT CACCACTGTT GCCTCAGCAC 
CTTATAGAGC CTACTGACTA TGGAGAACCT CCAACTAGAG ACTCTAATCA TGATTTAGCA 
ACTGTAGAAG CCAGGTCGCC ATCCCCACCC CTTCCTCCCG GCAGGTCAAC GTCCCTATTG 
ATTCGTCCTG ATTATGCTCA TTCACAACCT GCGGCTGTGA AGTTAGGAGG AGCTGTTCCA 
GGTGAAACAG CAAAAAATAT ACTGAAACCA TCACCATTAA AAGGAACTGC AAACTCTGGC 
ATCCATAATC AATCTAGTCA TGATATGCAA GCAAAAAGTC TATCTTCTGG GGCCAAAATT 
GAGTTATCTT ACCTTCAAGA AAATTCTGAA GCAATTATGC AAAATAAATG TATATCTCAG 
CAACCCCATA GTGCATGCCA AGGACTTTCC AAAGTTTCCA CAAAGCAAGT CTGCCTGAAA 
AACACCAATC AGATGGGTCT CCACAGGAAC CGTGAGTTTC TGAGCATGGA TTCTCAGACA 
GGCAGGTCCA TTCCTCTGGG TCCGTCACTG GAAACAACTT CACAAGAAGT GCATTCCAGC 
AAGAAGGATA TTTCCAGTGA CAGTGGGGTT GATCAGCCAC AAAAGATGCC AAACATTCTC 
CCTGCAACTC CTCAATGTCA TACAGCAAGC GTGAGTGAGC CCTTGCTATC TCAGGTAAAC 
AATTCTCCAT TGTCAACACT GTTTCATGGT AGTGACACTG CAAATGCCAC ACAAAACTCT 
AATGAGAAAG GAGTGAAAAC CCGTCTCCCT GTAGGACTGA AATTATTTGT CAAATCTCCT 
CAACTTCTCC GAAAGAGCTC CACCGTCCCA GGGAAGCAGG AAAAAGATAG TATAAATGCA 
GCTTCCAAAA GCCACGTGAG TACAAGTAAG CACAAGCAAA ATGAACGTGT GGCCACCAAA 
GGTGCAATAG ATGCTGAATT AAAGGACTCT AAGTCTGATA CCGAAATAAA AGATAATTTT 
TCTTTGAGAC TTAGTGTGGA TACAGCATCA CCTCGTTCAC ATGAAAATGG CAGCTTGGTG 
GTAGACAGAG TAGATGAATT AGAAAGCAAA CTAGTTAAGA GATCTTTTTC TTCTAGCAAC 
AAGTCACACT TGAAACCAGC TCTAGGCATG AATGGAGCGA AAGCTCGAAG TCAGAGTTTC 
AGCGTTCATA CAGGAGAAAA GTCATCTACC CTTGTGACAG AAGGTCTTGG AAAAGTACGG 
ACTCAGATTA TTACAAACAC TTCTGACAGA GGAAATTCTC TAACAAGGCA GAACTCAGCC 
ATGGAAGGAC TTCAAATCAA GGCCATTCCT GGGTCAGGAG CAACACAAGA GACTCTTTCA 
AATACTTCAA AAAACACAGA AAATGCTTAC TCTAGACAAG GTTCTCTTGG AAGTAATAGC 
AATAACCAGC ATGGAAGCCC AAGCAAACTG CCATTCAGAT CATCTCCAAA AGTAGACTTA 
TTTCGAAACA CTTCAAAATA TGATGGAAAC AAATCATTTT CTATGAAAGA TGCACGGAGT 
CTATCTGTCC AGAGTGAGAA AAGCAGTGAA AATTTAAAAC ACGAAGCTCT AAATAAAAAG 
AGTGTTTTGC CAGCAGAATT GGAGTTAGAT ACATCAGCTA CTGTATCTTC CAAACAGATT 
TCATCAATTC ATAGCTTGGG TGGAATAAAA TGTCCGCATA AACTGGAAGC AATAAAATCA 
CAGAGACAGC AGATGTCTTT AAAGTTAGCA GAAGCCCCTC AGGTTACTGA TGTTTTTAGT 
TCACCTGCTC TGCAACCTAC AATAGAGGAG AAGGTCATGT TGTGCATCCA AGAAAATGTC 
CAGAAGGGTC AGGAACAAAA CAAATCTCCC ACTGTAGAGA CTAAACAAAA GACTGGGCCC 
TCTATAGCTA GTTGGTTTGG CTTTCGAAAG AGTAAGCTGC CAGCACTGAG CAGCAGGAAA 
CCTGACGTTC CTAAAACAAA GGTAGAAAAA AAAGAACCAA GAGTTTCAGG ATTTGGAATT 
AAGCAGTCGA AATTAGAGAG AAGAAAAGAA AAAAAGCAAA CTGAACAACA CTGTGAAGTA 
GAAAATGAAA TTAACAGGAA AACGGACAGC AGTGACTTTT TAGATGATGC TATGGAGTTT 
AAAATTTTGA AGCCATCACG AAACACGTCT GGCAAGATTG GATGTGAACA ACTCAGAGGT 
TCCTCCATAG CTACATATTC ATCAAAAGAC AGTTTTATGA AGGAACTTCT AAACAGAGTT 
GATAAGAAAG CAGCTCAGCA GACAGAAAGT GGATCAAATA ATGTTTCCTA CAGGAGTGTG 
TCGAAGGGCA GCTCCCAGGG CTCCTCTCTT CACAGCAACT CTATCAGCTC TCAAGGAAAC 
CACAAGAAAA ACAGCAACAC AAAAGCTGAT ATGGAAATAC AGAATCAGAC CCTGGCTAAC 
GTAGTCGCAG AAAACCTGCA AGAAGAAGAG GAGGACACCA TGACAAGGAC TACATGTCAG 
AGTCATGTGA TAGAGTCTTG CTGCCAGATG AGGACGTTGG ACAGCGGGAT TGGAACCTTC 
CCACTCCCCG ACTCAGGGAA CCGATCTGCG GGGCGACACG TGTCTAAGCA GGAATTAACC 
TTGGAAACAG AAGCCCTAGC ACCGTCTGAG CAAGTTCTTC TTTCAGCTCC TTCAGTAAAA 
GCCAAAACTC TTGAGCGAGA AGTGCCTTCA ACAGCTACGA GTCAGGAGTC TGTGGAAAGC 
ATGATTAGTC ACTCAACATC TGATCCAGCC ATGACTGCCA AAGGTATACG ACCTTTTCAA 
AGTCGCCTTC CAAAACCAGA TTCCTCAGGA ATAATTAACC TTGCAAAGCA AAGTGAACAA 
GAACCAAGTT CTGTGACCTC TACCTCATTA CAGCTTACTG AAGAAACTGT AGAAAATAGA 
AAAGTTCTTT CAGATTGGAC CACCAAGAAA ACTACAAAAG CAAAGGACAG AACTCTAAGA 
GTGTGCACTT ACTCTGCCAG CAGCAGTGAC GCTGAACCGG AGCCAGAGTA TGGAACAAAT 
TATTTTCAAA CTGCAGAGGA GATGTTAGTA GAGTTAACAA AGAGCAACAA ACAAGCTGAG 
CAGGAAAAAC AGAAGCAGAG AAAGTCAGTT TTGGGGAACC CGATGTCAAT CTTGGATTTA 
TACCAGCACA GTCTCTATGG CCATTTTGGA GAGGATGGAC CTGAACAATT AACACATTTC 
AGTTTAATTG AGCAGCTGAG TGAACCTTCC GGTAAAGACA GCAAGGACAA AGAGAGCCCA 
AGTAACCTGA AGCAAACTGA AGAAACAAAA GAAGATCCTC AGACTAGATT ATCTAAAATT 
TCCCTGGAGT CTCTCAATAA ATTTAACAGC AATAACGTCC TTTTATTAGA AAAGGAGAAG 
AACTGTCTGA ACAAAGCTGA AGGACAAAAG GAAGAAAGCG GAAAGAAGGA AGCATCTTCC 
TTAAACAGCT CCGATAGGCA GGACATGGAC AATTTGGAGT CCCTGAGCGA TTCTTTGTAC 
GACAGCTTCT CCTCTTGTGC CAGCCAAGGC TCTAACGACG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015145420.1
CDS	8..6052
Translation	MREKLIHELEEERHLRLESEKRLREVTLESERNRAQMRGLQEQFSRMEETVRNLLQSQGSPGQNGEGTVNIMKAYQEKLSEDSRKCKEVMEKNHTAVDEDSRNESNSTEEEKEKTKLLLERLKALEAENSALALENENQREQYERCLDEVANQVVQALLTQKDLREECIKLKTRVFDLEQQNQTLSVLFQQRVKPASDLLLQKLHSRILDLSSGDLLSEVDRNRSSVPSRPADAQIHECQQNVKSTIPVLKCHSQLNVTGPSRLYPRSSCSSSELSLSSACSEYSSGSSYTWNDGKTCSKRSSVSWDKRISIGSSLPSNLSSPADDLPPTRIKENHILEGLKKLQRQKVLLESPSVIPKWGYKDCMNSNEGIYSPGVKCGSHHEQTHCKPMEIGSSCIEHNKTFSYDSDSHDDADDDSSSLLLLQEVPSKDCRIYCNKLTHSVSDSLFHWDPNRKHLPERSSYFNSKERPEKLTSFVSGFQAEVESCTRTKLPDLQLDQSHANIGWKDLNFQLSDTDDNEVLDELHIESSDEKSPSDLLATPFTEKYTKNSDVLIVTENSQFISSDKEERQTSAHSDIRPKTCNFIKQQKVIKKTSSEECITVIFDAEDGEPIEFSSHQIGVVTVTRNEISVNQPESRSSAEYTELIPQGIANLQAGTNARDYSVLEGPEDKTKKQTLEDNTGNKNMVVTTTCSESSPCGRIILQNAPRQKLVKPVYDVSYKTNSSSIVHAGVNQKPILTKIPSRGKFSPQKNKMTESEEAPVATSVTPATLEKSPALPPVKLSRLKKAQSPPRNFDSKVDFQVPKPPAHPLPSSKRPSRGNGTKYPKSQSPASPLLPQHLIEPTDYGEPPTRDSNHDLATVEARSPSPPLPPGRSTSLLIRPDYAHSQPAAVKLGGAVPGETAKNILKPSPLKGTANSGIHNQSSHDMQAKSLSSGAKIELSYLQENSEAIMQNKCISQQPHSACQGLSKVSTKQVCLKNTNQMGLHRNREFLSMDSQTGRSIPLGPSLETTSQEVHSSKKDISSDSGVDQPQKMPNILPATPQCHTASVSEPLLSQVNNSPLSTLFHGSDTANATQNSNEKGVKTRLPVGLKLFVKSPQLLRKSSTVPGKQEKDSINAASKSHVSTSKHKQNERVATKGAIDAELKDSKSDTEIKDNFSLRLSVDTASPRSHENGSLVVDRVDELESKLVKRSFSSSNKSHLKPALGMNGAKARSQSFSVHTGEKSSTLVTEGLGKVRTQIITNTSDRGNSLTRQNSAMEGLQIKAIPGSGATQETLSNTSKNTENAYSRQGSLGSNSNNQHGSPSKLPFRSSPKVDLFRNTSKYDGNKSFSMKDARSLSVQSEKSSENLKHEALNKKSVLPAELELDTSATVSSKQISSIHSLGGIKCPHKLEAIKSQRQQMSLKLAEAPQVTDVFSSPALQPTIEEKVMLCIQENVQKGQEQNKSPTVETKQKTGPSIASWFGFRKSKLPALSSRKPDVPKTKVEKKEPRVSGFGIKQSKLERRKEKKQTEQHCEVENEINRKTDSSDFLDDAMEFKILKPSRNTSGKIGCEQLRGSSIATYSSKDSFMKELLNRVDKKAAQQTESGSNNVSYRSVSKGSSQGSSLHSNSISSQGNHKKNSNTKADMEIQNQTLANVVAENLQEEEEDTMTRTTCQSHVIESCCQMRTLDSGIGTFPLPDSGNRSAGRHVSKQELTLETEALAPSEQVLLSAPSVKAKTLEREVPSTATSQESVESMISHSTSDPAMTAKGIRPFQSRLPKPDSSGIINLAKQSEQEPSSVTSTSLQLTEETVENRKVLSDWTTKKTTKAKDRTLRVCTYSASSSDAEPEPEYGTNYFQTAEEMLVELTKSNKQAEQEKQKQRKSVLGNPMSILDLYQHSLYGHFGEDGPEQLTHFSLIEQLSEPSGKDSKDKESPSNLKQTEETKEDPQTRLSKISLESLNKFNSNNVLLLEKEKNCLNKAEGQKEESGKKEASSLNSSDRQDMDNLESLSDSLYDSFSSCASQGSNDV

Target ORF information:

RefSeq Version	XM_015289934.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus NCK associated protein 5 (NCKAP5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015289934.1

ORF Insert Sequence:

ATGCGCGAGA AACTGATACA TGAGTTGGAG GAAGAAAGAC ATTTAAGACT TGAGAGTGAA 
AAACGATTAC GGGAGGTAAC ACTGGAGTCA GAGCGTAACA GAGCTCAGAT GCGTGGCCTA 
CAAGAGCAGT TTTCCAGAAT GGAGGAAACG GTGAGAAATC TACTACAGAG CCAAGGATCC 
CCAGGCCAGA ATGGAGAAGG AACTGTCAAT ATCATGAAGG CATATCAGGA AAAGCTGTCA 
GAAGACAGTA GGAAATGCAA AGAAGTCATG GAAAAAAATC ACACAGCAGT AGATGAAGAT 
TCAAGAAATG AAAGCAACAG CACTGAAGAG GAAAAAGAAA AGACAAAATT GCTACTGGAG 
CGTTTGAAAG CTCTGGAGGC AGAGAATTCA GCACTGGCTC TGGAAAATGA AAATCAGAGG 
GAACAGTATG AAAGATGTCT TGATGAGGTA GCTAATCAGG TCGTCCAGGC TCTACTTACT 
CAAAAGGATC TGAGGGAGGA ATGTATAAAG CTGAAAACGA GAGTTTTTGA TTTGGAGCAA 
CAGAATCAAA CATTAAGTGT GCTTTTCCAG CAGAGAGTCA AACCAGCTTC TGACCTCCTC 
CTTCAGAAAC TCCATTCGCG CATCTTAGAC CTCTCATCTG GAGATTTGCT GTCAGAAGTA 
GATCGAAACA GAAGCTCGGT GCCGTCACGA CCTGCTGATG CTCAGATTCA CGAATGCCAG 
CAGAATGTGA AATCCACTAT ACCTGTCTTG AAATGCCACA GTCAATTAAA TGTGACAGGT 
CCTAGCCGCC TGTATCCCCG GAGCAGCTGC AGTAGCAGTG AACTCTCCCT TTCAAGTGCT 
TGCAGTGAAT ATTCCAGTGG TTCCTCCTAC ACTTGGAATG ATGGGAAGAC GTGCAGCAAA 
AGGTCATCCG TGAGCTGGGA TAAAAGAATA AGCATTGGTT CTTCACTCCC AAGCAACCTC 
TCTAGTCCTG CAGATGATCT GCCTCCAACT CGTATCAAGG AAAACCATAT CCTAGAAGGA 
CTGAAGAAAT TACAGAGGCA AAAAGTATTA CTTGAATCAC CTTCAGTAAT ACCGAAATGG 
GGTTACAAAG ATTGCATGAA CTCCAATGAA GGAATATATT CCCCTGGAGT TAAATGTGGC 
AGCCATCATG AGCAGACTCA TTGTAAGCCA ATGGAAATAG GAAGTAGTTG CATTGAACAT 
AACAAAACTT TCTCTTATGA TTCAGACTCA CATGATGATG CAGATGATGA TTCTTCATCT 
TTATTATTGC TGCAGGAAGT ACCAAGCAAA GACTGCAGGA TCTATTGTAA CAAATTAACT 
CACAGTGTTT CTGACAGTCT CTTTCACTGG GATCCTAATA GGAAACATTT GCCAGAAAGA 
AGTTCATATT TTAATTCAAA GGAAAGACCA GAAAAATTGA CCAGTTTTGT CAGTGGATTT 
CAGGCAGAAG TAGAATCGTG CACCAGAACA AAGCTGCCTG ATTTACAGTT AGATCAAAGC 
CATGCTAATA TAGGCTGGAA GGACCTTAAT TTTCAGCTTT CAGATACTGA TGACAATGAA 
GTCTTGGATG AATTGCATAT AGAAAGCAGT GATGAGAAAA GTCCATCAGA TTTATTAGCA 
ACTCCTTTTA CTGAGAAGTA CACAAAGAAC TCTGATGTGC TCATAGTCAC AGAGAATTCT 
CAGTTTATAT CTAGTGACAA AGAGGAAAGA CAGACATCTG CTCACTCAGA CATTAGGCCA 
AAGACATGTA ATTTCATTAA ACAACAAAAA GTCATAAAAA AGACTTCTTC TGAGGAGTGC 
ATTACAGTGA TATTTGATGC TGAGGACGGT GAGCCTATTG AATTCAGCTC GCATCAAATT 
GGAGTTGTCA CTGTAACAAG AAATGAGATA TCAGTCAACC AACCAGAAAG TAGGTCCAGT 
GCAGAATATA CTGAACTTAT ACCTCAGGGA ATAGCTAATT TGCAGGCAGG AACAAATGCT 
AGAGACTACA GTGTTTTGGA AGGACCTGAA GACAAAACTA AAAAACAGAC TCTTGAAGAT 
AATACAGGGA ATAAAAATAT GGTTGTCACC ACAACCTGCA GTGAATCTTC ACCCTGTGGA 
AGAATCATAC TGCAAAATGC ACCACGACAA AAACTAGTGA AGCCAGTGTA TGATGTGTCG 
TACAAGACCA ATTCAAGCTC CATTGTTCAT GCAGGAGTCA ATCAAAAGCC AATCTTGACT 
AAAATACCCA GCAGAGGTAA ATTTTCTCCA CAAAAAAACA AGATGACAGA GAGTGAGGAG 
GCACCTGTAG CTACTTCAGT GACCCCTGCA ACCCTTGAAA AATCACCTGC TCTTCCACCT 
GTAAAGCTTT CAAGACTCAA AAAGGCACAA AGTCCACCTC GTAATTTTGA CTCAAAAGTT 
GACTTCCAAG TTCCAAAGCC CCCTGCCCAC CCCCTGCCCA GCTCAAAGCG TCCGAGCAGA 
GGCAATGGGA CAAAGTATCC AAAGAGTCAG AGTCCAGCAT CACCACTGTT GCCTCAGCAC 
CTTATAGAGC CTACTGACTA TGGAGAACCT CCAACTAGAG ACTCTAATCA TGATTTAGCA 
ACTGTAGAAG CCAGGTCGCC ATCCCCACCC CTTCCTCCCG GCAGGTCAAC GTCCCTATTG 
ATTCGTCCTG ATTATGCTCA TTCACAACCT GCGGCTGTGA AGTTAGGAGG AGCTGTTCCA 
GGTGAAACAG CAAAAAATAT ACTGAAACCA TCACCATTAA AAGGAACTGC AAACTCTGGC 
ATCCATAATC AATCTAGTCA TGATATGCAA GCAAAAAGTC TATCTTCTGG GGCCAAAATT 
GAGTTATCTT ACCTTCAAGA AAATTCTGAA GCAATTATGC AAAATAAATG TATATCTCAG 
CAACCCCATA GTGCATGCCA AGGACTTTCC AAAGTTTCCA CAAAGCAAGT CTGCCTGAAA 
AACACCAATC AGATGGGTCT CCACAGGAAC CGTGAGTTTC TGAGCATGGA TTCTCAGACA 
GGCAGGTCCA TTCCTCTGGG TCCGTCACTG GAAACAACTT CACAAGAAGT GCATTCCAGC 
AAGAAGGATA TTTCCAGTGA CAGTGGGGTT GATCAGCCAC AAAAGATGCC AAACATTCTC 
CCTGCAACTC CTCAATGTCA TACAGCAAGC GTGAGTGAGC CCTTGCTATC TCAGGTAAAC 
AATTCTCCAT TGTCAACACT GTTTCATGGT AGTGACACTG CAAATGCCAC ACAAAACTCT 
AATGAGAAAG GAGTGAAAAC CCGTCTCCCT GTAGGACTGA AATTATTTGT CAAATCTCCT 
CAACTTCTCC GAAAGAGCTC CACCGTCCCA GGGAAGCAGG AAAAAGATAG TATAAATGCA 
GCTTCCAAAA GCCACGTGAG TACAAGTAAG CACAAGCAAA ATGAACGTGT GGCCACCAAA 
GGTGCAATAG ATGCTGAATT AAAGGACTCT AAGTCTGATA CCGAAATAAA AGATAATTTT 
TCTTTGAGAC TTAGTGTGGA TACAGCATCA CCTCGTTCAC ATGAAAATGG CAGCTTGGTG 
GTAGACAGAG TAGATGAATT AGAAAGCAAA CTAGTTAAGA GATCTTTTTC TTCTAGCAAC 
AAGTCACACT TGAAACCAGC TCTAGGCATG AATGGAGCGA AAGCTCGAAG TCAGAGTTTC 
AGCGTTCATA CAGGAGAAAA GTCATCTACC CTTGTGACAG AAGGTCTTGG AAAAGTACGG 
ACTCAGATTA TTACAAACAC TTCTGACAGA GGAAATTCTC TAACAAGGCA GAACTCAGCC 
ATGGAAGGAC TTCAAATCAA GGCCATTCCT GGGTCAGGAG CAACACAAGA GACTCTTTCA 
AATACTTCAA AAAACACAGA AAATGCTTAC TCTAGACAAG GTTCTCTTGG AAGTAATAGC 
AATAACCAGC ATGGAAGCCC AAGCAAACTG CCATTCAGAT CATCTCCAAA AGTAGACTTA 
TTTCGAAACA CTTCAAAATA TGATGGAAAC AAATCATTTT CTATGAAAGA TGCACGGAGT 
CTATCTGTCC AGAGTGAGAA AAGCAGTGAA AATTTAAAAC ACGAAGCTCT AAATAAAAAG 
AGTGTTTTGC CAGCAGAATT GGAGTTAGAT ACATCAGCTA CTGTATCTTC CAAACAGATT 
TCATCAATTC ATAGCTTGGG TGGAATAAAA TGTCCGCATA AACTGGAAGC AATAAAATCA 
CAGAGACAGC AGATGTCTTT AAAGTTAGCA GAAGCCCCTC AGGTTACTGA TGTTTTTAGT 
TCACCTGCTC TGCAACCTAC AATAGAGGAG AAGGTCATGT TGTGCATCCA AGAAAATGTC 
CAGAAGGGTC AGGAACAAAA CAAATCTCCC ACTGTAGAGA CTAAACAAAA GACTGGGCCC 
TCTATAGCTA GTTGGTTTGG CTTTCGAAAG AGTAAGCTGC CAGCACTGAG CAGCAGGAAA 
CCTGACGTTC CTAAAACAAA GGTAGAAAAA AAAGAACCAA GAGTTTCAGG ATTTGGAATT 
AAGCAGTCGA AATTAGAGAG AAGAAAAGAA AAAAAGCAAA CTGAACAACA CTGTGAAGTA 
GAAAATGAAA TTAACAGGAA AACGGACAGC AGTGACTTTT TAGATGATGC TATGGAGTTT 
AAAATTTTGA AGCCATCACG AAACACGTCT GGCAAGATTG GATGTGAACA ACTCAGAGGT 
TCCTCCATAG CTACATATTC ATCAAAAGAC AGTTTTATGA AGGAACTTCT AAACAGAGTT 
GATAAGAAAG CAGCTCAGCA GACAGAAAGT GGATCAAATA ATGTTTCCTA CAGGAGTGTG 
TCGAAGGGCA GCTCCCAGGG CTCCTCTCTT CACAGCAACT CTATCAGCTC TCAAGGAAAC 
CACAAGAAAA ACAGCAACAC AAAAGCTGAT ATGGAAATAC AGAATCAGAC CCTGGCTAAC 
GTAGTCGCAG AAAACCTGCA AGAAGAAGAG GAGGACACCA TGACAAGGAC TACATGTCAG 
AGTCATGTGA TAGAGTCTTG CTGCCAGATG AGGACGTTGG ACAGCGGGAT TGGAACCTTC 
CCACTCCCCG ACTCAGGGAA CCGATCTGCG GGGCGACACG TGTCTAAGCA GGAATTAACC 
TTGGAAACAG AAGCCCTAGC ACCGTCTGAG CAAGTTCTTC TTTCAGCTCC TTCAGTAAAA 
GCCAAAACTC TTGAGCGAGA AGTGCCTTCA ACAGCTACGA GTCAGGAGTC TGTGGAAAGC 
ATGATTAGTC ACTCAACATC TGATCCAGCC ATGACTGCCA AAGGTATACG ACCTTTTCAA 
AGTCGCCTTC CAAAACCAGA TTCCTCAGGA ATAATTAACC TTGCAAAGCA AAGTGAACAA 
GAACCAAGTT CTGTGACCTC TACCTCATTA CAGCTTACTG AAGAAACTGT AGAAAATAGA 
AAAGTTCTTT CAGATTGGAC CACCAAGAAA ACTACAAAAG CAAAGGACAG AACTCTAAGA 
GTGTGCACTT ACTCTGCCAG CAGCAGTGAC GCTGAACCGG AGCCAGAGTA TGGAACAAAT 
TATTTTCAAA CTGCAGAGGA GATGTTAGTA GAGTTAACAA AGAGCAACAA ACAAGCTGAG 
CAGGAAAAAC AGAAGCAGAG AAAGTCAGTT TTGGGGAACC CGATGTCAAT CTTGGATTTA 
TACCAGCACA GTCTCTATGG CCATTTTGGA GAGGATGGAC CTGAACAATT AACACATTTC 
AGTTTAATTG AGCAGCTGAG TGAACCTTCC GGTAAAGACA GCAAGGACAA AGAGAGCCCA 
AGTAACCTGA AGCAAACTGA AGAAACAAAA GAAGATCCTC AGACTAGATT ATCTAAAATT 
TCCCTGGAGT CTCTCAATAA ATTTAACAGC AATAACGTCC TTTTATTAGA AAAGGAGAAG 
AACTGTCTGA ACAAAGCTGA AGGACAAAAG GAAGAAAGCG GAAAGAAGGA AGCATCTTCC 
TTAAACAGCT CCGATAGGCA GGACATGGAC AATTTGGAGT CCCTGAGCGA TTCTTTGTAC 
GACAGCTTCT CCTCTTGTGC CAGCCAAGGC TCTAACGACG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NCKAP5
Entrez Gene ID	424283
Full Name	NCK associated protein 5
General protein information	Preferred Names NCK associated protein 5 Names nck-associated protein 5
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Homo sapiens (human)	NCKAP5	NP_997246.2
Canis lupus familiaris (dog)	NCKAP5	XP_005631978.1
Rattus norvegicus (Norway rat)	Nckap5	XP_344135.4
Gallus gallus (chicken)	NCKAP5	XP_422129.4
Bos taurus (cattle)	NCKAP5	XP_002685464.2
Pan troglodytes (chimpanzee)	NCKAP5	XP_001151197.2
Macaca mulatta (Rhesus monkey)	NCKAP5	XP_001102812.2
Mus musculus (house mouse)	Nckap5	NP_001075225.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NCKAP5 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

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