DICER1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	DICER1
Entrez Gene ID	423437
Full Name	dicer 1, ribonuclease III
Synonyms	dicer
General protein information	Preferred Names dicer 1, ribonuclease III Names endoribonuclease Dicer dicer 1, ribonuclease type III helicase-moi
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001040465.1	NP_001035555.1	endoribonuclease Dicer

Pathways from BioSystems

Homologies

Homo sapiens (human)	DICER1	NP_085124.2
Rattus norvegicus (Norway rat)	Dicer1	XP_006240573.1
Danio rerio (zebrafish)	dicer1	NP_001154925.1
Magnaporthe oryzae (rice blast fungus)	MGG_01541	XP_003714515.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002836	XP_003436256.1
Neurospora crassa	NCU08270	XP_961898.1
Macaca mulatta (Rhesus monkey)	DICER1	XP_001100868.1
Gallus gallus (chicken)	DICER1	NP_001035555.1
Drosophila melanogaster (fruit fly)	Dcr-1	NP_524453.1
Caenorhabditis elegans (roundworm)	dcr-1	NP_498761.2
Xenopus tropicalis (tropical clawed frog)	dicer1	NP_001123390.2
Schizosaccharomyces pombe (fission yeast)	dcr1	NP_588215.2
Canis lupus familiaris (dog)	DICER1	XP_868526.3
Bos taurus (cattle)	DICER1	NP_976235.1
Pan troglodytes (chimpanzee)	DICER1	XP_001154369.1
Mus musculus (house mouse)	Dicer1	NP_683750.2

Gene Ontology
Bibliography

Related articles in PubMed

Dicer is essential for formation of the heterochromatin structure in vertebrate cells.
Fukagawa T, Nogami M, Yoshikawa M, Ikeno M, Okazaki T, Takami Y, Nakayama T, Oshimura M
Nature cell biology6(8)784-91(2004 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DICER1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DICER1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa28117	NM_001040465.1 Latest version!	Gallus gallus dicer 1, ribonuclease III (DICER1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DICER1 ( NM_001040465.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001035555.1 Gallus gallus dicer 1, ribonuclease III (DICER1), mRNA.

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CloneID	OGa28117
Clone ID Related Accession (Same CDS sequence)	NM_001040465.1
Accession Version	NM_001040465.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1544112000000
Organism	Gallus gallus(chicken)
Product	endoribonuclease Dicer
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB253768.1. On May 9, 2006 this sequence version replaced XM_421346.1. ##Evidence-Data-START## Transcript exon combination :: AB253768.1 [ECO:0000332] ##Evidence-Data-END##

ATGAAAAGCC CTGCTTTGCA ATCCCTCAGC ATGGCGGGAC TGCAGCTCAT GACCCCTGCT 
TCCTCCCCAA TGGGTCCATT TTTTGGACTA CCGTGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTTTGGA TCATAATACA 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACTTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG GGATTTCAAC AAAAACGGAA AAAGAACTGT GTTCTTGGTC 
AACTCAGCAA ATCAAGTTGC TCAGCAAGTG TCAGCTGTCA GGACACATTC AGATCTTAAA 
GTGGGAGAGT ATTCAAGTTT AGAGGTAACT GAATCATGGA CAAAAGAGAA ATGGAGTCAG 
GAATTCTCTA AGCATCAGGT TCTGGTTATG ACATGTCATG TTGCTTTGAC TGTTTTGAGA 
AATGAGTATT TATCCCTATC AAATATTAAT CTACTGGTGT TTGATGAGTG CCATCTTGCA 
ATCCAGGACC ATCCATACCG TGAAATTATG AAGATCTGTG AGGATTACCC ATCTTGCCCT 
CGAATCTTGG GATTAACTGC TTCCATTTTA AATGGGAAGT GTGATCCTGC TGAGTTAGAA 
GAAAAGATAA AGAAACTGGA GAAAATACTA AAGAGCAATG CTGAAACTGC AACTGATTTG 
GTGGTCTTAG ATAGATATAC TTCTCAGCCA TGTGAGATTG TTGTAGACTG TGGACCATAT 
ACTGACAAAA GTGGATTGTA CGGAAGACTA TTAAGGGAAT TAGATGAAGC ACTTCATTTT 
CTAAATGACT GCAACATATC TGTACATTCA AAGGAAAGAG ATTCTACGTT AATTTCTAAA 
CAGATATTGT CAGACTGTCG AGCTGTGTTG GTTGTTCTGG GACCCTGGTG TGCAGATAAG 
GTAGCTGGGA TGATGGTGAG AGAGCTACAG AAGTATATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTTAGGA AAATACATGC ACTCTGTGAA 
GAGCATTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCAAAAGT AATAAAGCTG 
CTTGAAATCT TGCGCAAATA CAAACCATAT GAACGGCAGC AGTTCGAAAG TGTTGAATGG 
TATAACAATA GAAATCAGGA TAATTATGTA TCATGGAGTG ATTCTGAAGA TGATGATGAA 
GATGAAGAGA TTGAGGAGAA AGAGAAGCCA GAGACTAATT TCCCATCTCC TTTTACGAAT 
ATTTTATGTG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTTCT AAATAGGTTG 
ATAAAAGAAG CTGGCAAACA GGATCCAGAA CTGGCTTACA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGCAAGAA TCAGCCTCGT AACAAGCAGA TGGAAGTAGA ATTCAGAAAG 
CAGGAAGAGG TTCTTAGAAA ATTTCGAGCA CACGAGACCA ATCTTCTTAT CGCTACCAGT 
ATTGTAGAAG AGGGTGTTGA CATACCAAAA TGCAATTTGG TGGTGCGTTT TGATTTGCCC 
ACAGAATACC GTTCCTATGT GCAGTCAAAA GGAAGAGCTA GAGCACCAAT TTCCAATTAT 
ATAATGTTAG CAGATACAGA CAAAATTAAA AGTTTTGAGG AAGATCTTAA GACATATAAA 
GCAATTGAGA AGATCCTGAG GAATAAATGC TCAAAATCTG TTGATACCAG CGAAACCGAA 
ACTGAACCCA TTGTTGATGA TGATGATGTG TTTCCACCCT ATGTGCTGAG GACAGATGAG 
AACAGTCCAA GAGTTACTAT CAACACTGCA ATTGGACACA TAAATAGGTA CTGCGCTAGA 
TTACCAAGTG ATCCATTTAC ACACCTAGCT CCCAAGTGTA AAACCCGAGA ACTACCTGAT 
CATACATTCT ACTCTACTCT CTACCTGCCA ATCAACTCAC CTCTTCGAGC ATCAATTGTT 
GGTCCTCCAA TGAGTTGTGC AAGATTGGCT GAGAGAGTTG TAGCTCTTAT TTGCTGTGAA 
AAACTGCACA AAATCGGTGA ACTGGATGAC CATTTGATGC CAGTTGGAAA AGAAACTGTT 
AAATATGAGG AAGAGCTTGA TTTACATGAT GAAGAAGAAA CCAGTGTGCC AGGAAGACCA 
GGCTCTACAA AAAGACGACA GTGCTACCCT AAAGCTATTC CAGAATGTTT GAGGGATAGT 
TATCCCAAAC CTGATCAGCC CTGTTACCTG TATGTAATAG GAATGGTGTT AACTACTCCA 
TTGCCTGATG AGCTCAACTT TAGGAGACGA AAGCTATATC CTCCTGAGGA TACGACGAGA 
TGTTTTGGCA TATTGACAGC CAAACCTATA CCTCAGATTC CTCACTTTCC TGTGTATACT 
CGCTCTGGAG AGGTTACAAT ATCTATTGAG CTTAAGAAAT CTGGTTTTAC TTTGTCTTTG 
CAAATGCTTG AGCTGATAAC ACGACTCCAC CAGTACATTT TCTCACATAT TCTTCGTCTT 
GAGAAACCTG CACTAGAGTT CAAACCCACA GAAGCTGATT CAGCCTATTG TGTTCTACCT 
CTTAACATTG TTGATGACTC CAGCACTTTG GACATTGACT TTAAGTTTAT GGAAGACATT 
GAAAAGTCTG AAGCGCGTAC AGGCATTCCC AGTACACAGT ATACAAAAGA AATGCCTTTT 
ATTTTCAAGT TAGAAGACTA CCAGGATGCA GTTATCATTC CACGGTATCG AAATTTTGAT 
CAGCCTCATC GATTCTATGT AGCTGATGTA TACACTGATC TTACTCCACT CAGTAAATTC 
CCTTCCCCTG AATATGAAAC TTTTGCTGAA TATTATAAAA CAAAATATAA TCTTGACCTG 
ACCAATCTCA ACCAGCCGCT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACTCCTCGCC ATTTGAATCA GAAGGGGAAA GCACTTCCAC TAAGCAGTGC TGAGAAGAGG 
AAAGCAAAAT GGGAAAGTCT GCAGAATAAG CAGATACTGG TTCCTGAGCT ATGTGCTATA 
CATCCTATTC CAGCATCGTT GTGGAGAAAA GCAGTTTGCC TCCCCAGCAT ACTTTATCGC 
CTGCACTGCC TTTTGACAGC AGAGGAACTA AGAGCTCAAA CAGCCACTGA TGCTGGCGTA 
GGGGTTAAAT CACTTCCTGC AGATTTCAGA TATCCTAACC TGGACTTCGG ATGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCCATA CCTAGCTCCT CTTTGGTAGA GAATGAAAAT 
TACTGTAAGC ACAGCACAAT TGTAGTCCCT GAAAATGCTG CACATCAAGG TGCTAATAGA 
ACCTCTTCTG CAGAAAAACA TGACCAAATG TCTGTGAGTT ACAGAACACT GCTTGATGAG 
TCACCCAGTA AACTCCAAAT TGATGTCTCA GCAGAACTTG CAGCAATTAA TGGTGTTTCT 
TATAATAAAA ATCTTGCCAA TGGCAATTGT GATTTAGTTA ACAGAGACTT TTGCCAAGGA 
AATCAGCTGA ATTACTGCAG GCAGGAAATA CCTGTACAAC CAACTACCTC ATATCCCATT 
CAGAATTTAT ACAGCAGTGA GAACCAGCCC AAGCCCAGCA ATGAATGTAC TCTACTGAGT 
AATAAATACC TTGATGGAAA TGCTAACAGA TCTACCTCAG ATGGATGCCC CAAAATGACA 
GTGACCACCA GTACTTCAAC AGCTCTTAAT CTTTCAAAAG ACAAAGTGGA TTCTGAAAAG 
AACACTTCCA GTGGGTACTC CTCAAAAACT CTTGGTCCTA ATCCTGGTCT CATTCTTCAG 
GCTTTGACTC TTTCAAATGC TAGTGATGGA TTTAATTTGG AGCGGCTAGA AATGCTTGGT 
GATTCATTTT TAAAGCATGC CATCACTACA TACTTGTTTT GCACTTACCC TGATGCACAC 
GAGGGACGGC TGTCATACAT GAGAAGTAAA AAAGTCAGCA ACTGTAATTT GTATCGCCTT 
GGAAAAAAGA AAGGACTGCC TAGTCGAATG GTGGTATCGA TTTTTGATCC CCCTGTCAAT 
TGGCTTCCTC CTGGTTACAT AGTAAACCAG GACAAGAGCA ACACTGACAA ATGGGAGAAA 
GAAGAAACGA CAAAGGAGAA TTTGTTGGCT AATGGTAAAC TTGATTATGA TGATGATGAC 
GAAGAGGATG AAGACCTAAT GTGGAGGTTG CCCAAGGAAG AAACAGACTT TGAAGATGAT 
TTTTTGGAGT ATGATCAAGA GCATATCAAA TTCATAGATA GTATGTTAAT GGGATCGGGG 
GCTTTTGTGA AGAAAATTTC TCTCTCTCAC TTTTCAACCA CTGATTCAAA CTATGAATGG 
AAAGCACCCA AAAAGTCATC CCTGGGTAAT GTTCCGTTTT CATCAGATTT TGATGATTTT 
GACTACAGCT CTTGGGATGC TATGTGCTAT CTGGATCCTA GCAAGGCTGT TGAAGAGGAT 
GATTTTGTAG TGGGTTTCTG GAATCCATCA GAAGAAAACT GTGGTGTCGA TGCAGGAAAA 
CAGTCTATCT CATATGATTT GCATACAGAG CAGTGTATTG CTGACAAAAG CATTGCAGAC 
TGTGTGGAAG CCCTGTTGGG CTGCTATCTG ACCAGCTGTG GTGAAAGAGC TGCTCAGCTT 
TTCCTGTGTT CACTGGGGCT GAAGGTGCTC CCTGTAATTA AAAAGACTGA TTGGGAAAGC 
ACTCTGTGTG CGACTGGAGA GAACTGTAAC AGTGAGCAGA AGAATCTTTC ACCAAACTCT 
GTTTCTGCTT GTATAGTTAA TTCAGAGCCT TCTCTTTATA AAGACCTGGA GTATGGTTGT 
TTGAAGATCC CACCAAGGTG TATGTTTGAT CACCCAGATG CTGAAAAAAC CCTGAATCAC 
CTTATTTCTG GTTTTGAAAA CTTTGAGAAG AAAATTAATT ACAGTTTTAA GAACAAGGCG 
TATCTTCTTC AGGCATTTAC TCATGCCTCA TACCATTATA ATACCATTAC TGATTGTTAC 
CAGCGCTTAG AATTCCTGGG AGATGCAATT TTGGACTACC TCATAACCAA GCACCTTTAT 
GAAGACCCAC GGCAACACTC ACCAGGAGTT CTTACTGACC TGCGATCTGC TCTAGTCAAT 
AATACCATTT TTGCATCGCT AGCTGTAAAG TATGACTACC ACAAGTACTT CAAAGCTGTC 
TCTCCTGAAC TGTTTCATGT TATTGATGAC TTTGTGCAGT TTCAAATGGA AAAGAATGAA 
ATGCAAGGAA TGGATTCAGA GCTCAGAAGA TCTGAAGAAG ATGAAGAAAA GGAGGAAGAC 
ATTGAAGTAC CAAAGGCCAT GGGGGATATA TTTGAGTCCC TTGCTGGTGC CATTTATATG 
GATAGTGGAA TGTCTTTGGA AATGGTTTGG CAAGTGTACT ATCCAATGAT GAGGCCATTA 
ATAGAAAAGT TTTCTGCTAA TGTGCCCCGT TCCCCAGTGC GAGAGCTGCT TGAGATGGAG 
CCAGAGACGG CCAAGTTTAG TCCTGCAGAA AGAACTTACG ATGGGAAGGT CAGAGTAACT 
GTGGAAGTTG TAGGAAAGGG AAAGTTTAAA GGTGTTGGCA GAAGCTACAG GATTGCCAAA 
TCTGCAGCTG CGAGAAGAGC ACTACGAAGC CTCAAAGCAA ATCAACCTCA GGTCCCAAAC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035555.1
CDS	22..5787
Translation	MKSPALQSLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNKNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSSLEVTESWTKEKWSQEFSKHQVLVMTCHVALTVLRNEYLSLSNINLLVFDECHLAIQDHPYREIMKICEDYPSCPRILGLTASILNGKCDPAELEEKIKKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPYTDKSGLYGRLLRELDEALHFLNDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEVEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTSETETEPIVDDDDVFPPYVLRTDENSPRVTINTAIGHINRYCARLPSDPFTHLAPKCKTRELPDHTFYSTLYLPINSPLRASIVGPPMSCARLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPKPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPTEADSAYCVLPLNIVDDSSTLDIDFKFMEDIEKSEARTGIPSTQYTKEMPFIFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTATDAGVGVKSLPADFRYPNLDFGWKKSIDSKSFISIPSSSLVENENYCKHSTIVVPENAAHQGANRTSSAEKHDQMSVSYRTLLDESPSKLQIDVSAELAAINGVSYNKNLANGNCDLVNRDFCQGNQLNYCRQEIPVQPTTSYPIQNLYSSENQPKPSNECTLLSNKYLDGNANRSTSDGCPKMTVTTSTSTALNLSKDKVDSEKNTSSGYSSKTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYIVNQDKSNTDKWEKEETTKENLLANGKLDYDDDDEEDEDLMWRLPKEETDFEDDFLEYDQEHIKFIDSMLMGSGAFVKKISLSHFSTTDSNYEWKAPKKSSLGNVPFSSDFDDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDAGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKKTDWESTLCATGENCNSEQKNLSPNSVSACIVNSEPSLYKDLEYGCLKIPPRCMFDHPDAEKTLNHLISGFENFEKKINYSFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQMEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLEMVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	NM_001040465.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus dicer 1, ribonuclease III (DICER1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001040465.1

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ATCCCTCAGC ATGGCGGGAC TGCAGCTCAT GACCCCTGCT 
TCCTCCCCAA TGGGTCCATT TTTTGGACTA CCGTGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTTTGGA TCATAATACA 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACTTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCCTATC AGATCAGGGG GGATTTCAAC AAAAACGGAA AAAGAACTGT GTTCTTGGTC 
AACTCAGCAA ATCAAGTTGC TCAGCAAGTG TCAGCTGTCA GGACACATTC AGATCTTAAA 
GTGGGAGAGT ATTCAAGTTT AGAGGTAACT GAATCATGGA CAAAAGAGAA ATGGAGTCAG 
GAATTCTCTA AGCATCAGGT TCTGGTTATG ACATGTCATG TTGCTTTGAC TGTTTTGAGA 
AATGAGTATT TATCCCTATC AAATATTAAT CTACTGGTGT TTGATGAGTG CCATCTTGCA 
ATCCAGGACC ATCCATACCG TGAAATTATG AAGATCTGTG AGGATTACCC ATCTTGCCCT 
CGAATCTTGG GATTAACTGC TTCCATTTTA AATGGGAAGT GTGATCCTGC TGAGTTAGAA 
GAAAAGATAA AGAAACTGGA GAAAATACTA AAGAGCAATG CTGAAACTGC AACTGATTTG 
GTGGTCTTAG ATAGATATAC TTCTCAGCCA TGTGAGATTG TTGTAGACTG TGGACCATAT 
ACTGACAAAA GTGGATTGTA CGGAAGACTA TTAAGGGAAT TAGATGAAGC ACTTCATTTT 
CTAAATGACT GCAACATATC TGTACATTCA AAGGAAAGAG ATTCTACGTT AATTTCTAAA 
CAGATATTGT CAGACTGTCG AGCTGTGTTG GTTGTTCTGG GACCCTGGTG TGCAGATAAG 
GTAGCTGGGA TGATGGTGAG AGAGCTACAG AAGTATATCA AACATGAGCA AGAGGAGCTG 
CACAGGAAAT TTTTATTGTT TACAGACACT TTCCTTAGGA AAATACATGC ACTCTGTGAA 
GAGCATTTCT CACCTGCCTC ACTTGACCTG AAATTTGTAA CTCCAAAAGT AATAAAGCTG 
CTTGAAATCT TGCGCAAATA CAAACCATAT GAACGGCAGC AGTTCGAAAG TGTTGAATGG 
TATAACAATA GAAATCAGGA TAATTATGTA TCATGGAGTG ATTCTGAAGA TGATGATGAA 
GATGAAGAGA TTGAGGAGAA AGAGAAGCCA GAGACTAATT TCCCATCTCC TTTTACGAAT 
ATTTTATGTG GAATTATTTT TGTGGAAAGA AGATACACAG CAGTTGTTCT AAATAGGTTG 
ATAAAAGAAG CTGGCAAACA GGATCCAGAA CTGGCTTACA TCAGTAGCAA TTTCATAACT 
GGACATGGCA TTGGCAAGAA TCAGCCTCGT AACAAGCAGA TGGAAGTAGA ATTCAGAAAG 
CAGGAAGAGG TTCTTAGAAA ATTTCGAGCA CACGAGACCA ATCTTCTTAT CGCTACCAGT 
ATTGTAGAAG AGGGTGTTGA CATACCAAAA TGCAATTTGG TGGTGCGTTT TGATTTGCCC 
ACAGAATACC GTTCCTATGT GCAGTCAAAA GGAAGAGCTA GAGCACCAAT TTCCAATTAT 
ATAATGTTAG CAGATACAGA CAAAATTAAA AGTTTTGAGG AAGATCTTAA GACATATAAA 
GCAATTGAGA AGATCCTGAG GAATAAATGC TCAAAATCTG TTGATACCAG CGAAACCGAA 
ACTGAACCCA TTGTTGATGA TGATGATGTG TTTCCACCCT ATGTGCTGAG GACAGATGAG 
AACAGTCCAA GAGTTACTAT CAACACTGCA ATTGGACACA TAAATAGGTA CTGCGCTAGA 
TTACCAAGTG ATCCATTTAC ACACCTAGCT CCCAAGTGTA AAACCCGAGA ACTACCTGAT 
CATACATTCT ACTCTACTCT CTACCTGCCA ATCAACTCAC CTCTTCGAGC ATCAATTGTT 
GGTCCTCCAA TGAGTTGTGC AAGATTGGCT GAGAGAGTTG TAGCTCTTAT TTGCTGTGAA 
AAACTGCACA AAATCGGTGA ACTGGATGAC CATTTGATGC CAGTTGGAAA AGAAACTGTT 
AAATATGAGG AAGAGCTTGA TTTACATGAT GAAGAAGAAA CCAGTGTGCC AGGAAGACCA 
GGCTCTACAA AAAGACGACA GTGCTACCCT AAAGCTATTC CAGAATGTTT GAGGGATAGT 
TATCCCAAAC CTGATCAGCC CTGTTACCTG TATGTAATAG GAATGGTGTT AACTACTCCA 
TTGCCTGATG AGCTCAACTT TAGGAGACGA AAGCTATATC CTCCTGAGGA TACGACGAGA 
TGTTTTGGCA TATTGACAGC CAAACCTATA CCTCAGATTC CTCACTTTCC TGTGTATACT 
CGCTCTGGAG AGGTTACAAT ATCTATTGAG CTTAAGAAAT CTGGTTTTAC TTTGTCTTTG 
CAAATGCTTG AGCTGATAAC ACGACTCCAC CAGTACATTT TCTCACATAT TCTTCGTCTT 
GAGAAACCTG CACTAGAGTT CAAACCCACA GAAGCTGATT CAGCCTATTG TGTTCTACCT 
CTTAACATTG TTGATGACTC CAGCACTTTG GACATTGACT TTAAGTTTAT GGAAGACATT 
GAAAAGTCTG AAGCGCGTAC AGGCATTCCC AGTACACAGT ATACAAAAGA AATGCCTTTT 
ATTTTCAAGT TAGAAGACTA CCAGGATGCA GTTATCATTC CACGGTATCG AAATTTTGAT 
CAGCCTCATC GATTCTATGT AGCTGATGTA TACACTGATC TTACTCCACT CAGTAAATTC 
CCTTCCCCTG AATATGAAAC TTTTGCTGAA TATTATAAAA CAAAATATAA TCTTGACCTG 
ACCAATCTCA ACCAGCCGCT GCTGGATGTG GACCACACAT CTTCAAGACT TAATCTTTTG 
ACTCCTCGCC ATTTGAATCA GAAGGGGAAA GCACTTCCAC TAAGCAGTGC TGAGAAGAGG 
AAAGCAAAAT GGGAAAGTCT GCAGAATAAG CAGATACTGG TTCCTGAGCT ATGTGCTATA 
CATCCTATTC CAGCATCGTT GTGGAGAAAA GCAGTTTGCC TCCCCAGCAT ACTTTATCGC 
CTGCACTGCC TTTTGACAGC AGAGGAACTA AGAGCTCAAA CAGCCACTGA TGCTGGCGTA 
GGGGTTAAAT CACTTCCTGC AGATTTCAGA TATCCTAACC TGGACTTCGG ATGGAAAAAA 
TCTATTGACA GCAAATCTTT CATCTCCATA CCTAGCTCCT CTTTGGTAGA GAATGAAAAT 
TACTGTAAGC ACAGCACAAT TGTAGTCCCT GAAAATGCTG CACATCAAGG TGCTAATAGA 
ACCTCTTCTG CAGAAAAACA TGACCAAATG TCTGTGAGTT ACAGAACACT GCTTGATGAG 
TCACCCAGTA AACTCCAAAT TGATGTCTCA GCAGAACTTG CAGCAATTAA TGGTGTTTCT 
TATAATAAAA ATCTTGCCAA TGGCAATTGT GATTTAGTTA ACAGAGACTT TTGCCAAGGA 
AATCAGCTGA ATTACTGCAG GCAGGAAATA CCTGTACAAC CAACTACCTC ATATCCCATT 
CAGAATTTAT ACAGCAGTGA GAACCAGCCC AAGCCCAGCA ATGAATGTAC TCTACTGAGT 
AATAAATACC TTGATGGAAA TGCTAACAGA TCTACCTCAG ATGGATGCCC CAAAATGACA 
GTGACCACCA GTACTTCAAC AGCTCTTAAT CTTTCAAAAG ACAAAGTGGA TTCTGAAAAG 
AACACTTCCA GTGGGTACTC CTCAAAAACT CTTGGTCCTA ATCCTGGTCT CATTCTTCAG 
GCTTTGACTC TTTCAAATGC TAGTGATGGA TTTAATTTGG AGCGGCTAGA AATGCTTGGT 
GATTCATTTT TAAAGCATGC CATCACTACA TACTTGTTTT GCACTTACCC TGATGCACAC 
GAGGGACGGC TGTCATACAT GAGAAGTAAA AAAGTCAGCA ACTGTAATTT GTATCGCCTT 
GGAAAAAAGA AAGGACTGCC TAGTCGAATG GTGGTATCGA TTTTTGATCC CCCTGTCAAT 
TGGCTTCCTC CTGGTTACAT AGTAAACCAG GACAAGAGCA ACACTGACAA ATGGGAGAAA 
GAAGAAACGA CAAAGGAGAA TTTGTTGGCT AATGGTAAAC TTGATTATGA TGATGATGAC 
GAAGAGGATG AAGACCTAAT GTGGAGGTTG CCCAAGGAAG AAACAGACTT TGAAGATGAT 
TTTTTGGAGT ATGATCAAGA GCATATCAAA TTCATAGATA GTATGTTAAT GGGATCGGGG 
GCTTTTGTGA AGAAAATTTC TCTCTCTCAC TTTTCAACCA CTGATTCAAA CTATGAATGG 
AAAGCACCCA AAAAGTCATC CCTGGGTAAT GTTCCGTTTT CATCAGATTT TGATGATTTT 
GACTACAGCT CTTGGGATGC TATGTGCTAT CTGGATCCTA GCAAGGCTGT TGAAGAGGAT 
GATTTTGTAG TGGGTTTCTG GAATCCATCA GAAGAAAACT GTGGTGTCGA TGCAGGAAAA 
CAGTCTATCT CATATGATTT GCATACAGAG CAGTGTATTG CTGACAAAAG CATTGCAGAC 
TGTGTGGAAG CCCTGTTGGG CTGCTATCTG ACCAGCTGTG GTGAAAGAGC TGCTCAGCTT 
TTCCTGTGTT CACTGGGGCT GAAGGTGCTC CCTGTAATTA AAAAGACTGA TTGGGAAAGC 
ACTCTGTGTG CGACTGGAGA GAACTGTAAC AGTGAGCAGA AGAATCTTTC ACCAAACTCT 
GTTTCTGCTT GTATAGTTAA TTCAGAGCCT TCTCTTTATA AAGACCTGGA GTATGGTTGT 
TTGAAGATCC CACCAAGGTG TATGTTTGAT CACCCAGATG CTGAAAAAAC CCTGAATCAC 
CTTATTTCTG GTTTTGAAAA CTTTGAGAAG AAAATTAATT ACAGTTTTAA GAACAAGGCG 
TATCTTCTTC AGGCATTTAC TCATGCCTCA TACCATTATA ATACCATTAC TGATTGTTAC 
CAGCGCTTAG AATTCCTGGG AGATGCAATT TTGGACTACC TCATAACCAA GCACCTTTAT 
GAAGACCCAC GGCAACACTC ACCAGGAGTT CTTACTGACC TGCGATCTGC TCTAGTCAAT 
AATACCATTT TTGCATCGCT AGCTGTAAAG TATGACTACC ACAAGTACTT CAAAGCTGTC 
TCTCCTGAAC TGTTTCATGT TATTGATGAC TTTGTGCAGT TTCAAATGGA AAAGAATGAA 
ATGCAAGGAA TGGATTCAGA GCTCAGAAGA TCTGAAGAAG ATGAAGAAAA GGAGGAAGAC 
ATTGAAGTAC CAAAGGCCAT GGGGGATATA TTTGAGTCCC TTGCTGGTGC CATTTATATG 
GATAGTGGAA TGTCTTTGGA AATGGTTTGG CAAGTGTACT ATCCAATGAT GAGGCCATTA 
ATAGAAAAGT TTTCTGCTAA TGTGCCCCGT TCCCCAGTGC GAGAGCTGCT TGAGATGGAG 
CCAGAGACGG CCAAGTTTAG TCCTGCAGAA AGAACTTACG ATGGGAAGGT CAGAGTAACT 
GTGGAAGTTG TAGGAAAGGG AAAGTTTAAA GGTGTTGGCA GAAGCTACAG GATTGCCAAA 
TCTGCAGCTG CGAGAAGAGC ACTACGAAGC CTCAAAGCAA ATCAACCTCA GGTCCCAAAC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dicer is essential for formation of the heterochromatin structure in vertebrate cells.
Nature cell biology6(8)784-91(2004 Aug)
Fukagawa T,Nogami M,Yoshikawa M,Ikeno M,Okazaki T,Takami Y,Nakayama T,Oshimura M

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DICER1 cDNA ORF clone, Gallus gallus(chicken)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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