ISM1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	ISM1
Entrez Gene ID	416740
Full Name	isthmin 1
Synonyms	C20orf82,ISM,Isthmin,bA149I18.1,dJ1077I2.1,isthmin-1
General protein information	Preferred Names isthmin 1 Names isthmin-1 isthmin 1 homolog isthmin 1, angiogenesis inhibitor
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015283524.1	XP_015139010.1	isthmin-1 isoform X1
XM_015283525.2	XP_015139011.1	isthmin-1 isoform X2
XM_415036.5	XP_415036.5	isthmin-1 isoform X1
XM_015283524.2	XP_015139010.1	isthmin-1 isoform X1
XM_015283525.1	XP_015139011.1	isthmin-1 isoform X2
XM_415036.6	XP_415036.5	isthmin-1 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ism1	NP_001263418.1
Pan troglodytes (chimpanzee)	ISM1	XP_525268.1
Bos taurus (cattle)	ISM1	NP_001157407.1
Gallus gallus (chicken)	ISM1	XP_415036.4
Xenopus tropicalis (tropical clawed frog)	LOC100487287	XP_002931787.1
Homo sapiens (human)	ISM1	NP_543016.1
Canis lupus familiaris (dog)	ISM1	XP_542888.2
Macaca mulatta (Rhesus monkey)	ISM1	XP_001083877.1
Rattus norvegicus (Norway rat)	Ism1	NP_001128024.1
Danio rerio (zebrafish)	ism1	NP_001012376.1

Gene Ontology

The following ISM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ISM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa33241	XM_415036.6 Latest version!	Gallus gallus isthmin 1 (ISM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OGa33241	XM_015283524.2 Latest version!	Gallus gallus isthmin 1 (ISM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OGa33242	XM_015283525.2 Latest version!	Gallus gallus isthmin 1 (ISM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OGa33241	XM_015283524.1	Gallus gallus isthmin 1, angiogenesis inhibitor (ISM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OGa33242	XM_015283525.1	Gallus gallus isthmin 1, angiogenesis inhibitor (ISM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OGa33241	XM_415036.5	Gallus gallus isthmin 1, angiogenesis inhibitor (ISM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ISM1 ( XM_015283524.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139010.1 Gallus gallus isthmin 1, angiogenesis inhibitor (ISM1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa33241
Clone ID Related Accession (Same CDS sequence)	XM_015283524.1 , XM_415036.5 , XM_015283524.2 , XM_415036.6
Accession Version	XM_015283524.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	isthmin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455832.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGGGG GACGGCAGGC AGCGGCGGAG CTGCGGAGAG AGAGAAGGGA AAGGGGGGGA 
GCGAGCACGG GGGAGGACAG CAGGGGAGGG AGGCAGGGCA CTATAAAAGC ATCCGCCGCA 
CACCTCTCCC GGCGCAGAGC GAGGGGGCGA GAAAGTTGGT GCGGGAGCAG CAGCGGCAGC 
GGGACGAGAG CCGACCGCCC GCCCGCACAC ACGCACGCAC GGTGGCAGGA GAGAGTGCGG 
CGCTGCCCCT GCCCAGCCCT AGGAGACAGA GAGAAGGGAT GGAGCCAGGT ACCCAGCTCC 
CATCGAGGGC AGCCGGCTGA GCCTCCCGCA GTCCCGCGGC CCGGGGAGCC CTCAGCAAGG 
AGGAGGAGGA AGGAGAGGAG GAGGAGGAGG AGGAAGAGGA GGGGGTGGGG GGGCTGCCCC 
TCATTGCCCC CCTCTCGCCG GGAGGGGCCG GGCCGGGAGC CGCGGGGCGC CCTGACGGAG 
GGAGGGAGGG ACGGGGGGAG CGGCGGGGCG GGCGAGGACA CGGCGGCGGG GGGGCAGCTC 
CGGGGCCACC CCCATCCCCT CGCGAGGGGG GAGCCGAGAG CCCCCCGCTC TCTCCTCCCC 
TCGGGGCTTC CCCGCCATGT GAGAGCCGCC CCCCGCCTCT CCTCCCTCCC GCGCACACAC 
GCATACACAC GCGCGCGGCT CCCGGCTCCC CCCGGCCCCG CGGCCCGCTC GCTCCGTCCC 
TCCGACGGCC CACGGTGCCT CTGGCTTGCA GGGCTGCTCG CGGAGACGCC GCCTGCCCGC 
GGCCCCGCCG CTACCTGCCG CCCGCCGCGG GGCAGCCACG GGATTCCCGC CGCCGCCTCC 
GCCGCGTCCC GCCCCGACGT GGCTCCGCCG TGGTGTGGCG GCCCCCGCTG CTGCTGCTGC 
TGCTGCTGCC GCCGCTGCCC CGCCGACGCC GAGAGCGGCG CGGTCCCGGC GGCCGACCCC 
GGGGCTGCTC CGGCGGCGGC GGCGGCTCCG GCGGCGGCGG CCGCGGCTCC TCCTCCTCCT 
CCGCCTCCTC CTCGGCCGCC GCCGCCGCGG GGCCCTAAAG CCGCCCGGCT CAAGAGGATG 
GTGCGGCTGG CGGCGGAGCT GCTGCTGCTG CTGGGGCTGC TCCTGCTCAC CTTGCACATC 
ACCGTGCTGC GCGGCGGCGG CGGAGAGCCG CAGGGCCCCG CGGGCGCCGC CAACCACAGC 
CAGCGGCAGA ACACGCTCAC TGCAGATGAC CATTCTCCTG AAGACAGCTT TACCCTGAAC 
CCTGAGGACA GAAGAGAATA CCCTGATCTC CAGGCTGCCC ACCGGCCATT CCCTAAGCAA 
CGATTCAGGC AGGAAAATGG GCATACATCC TTACAAAGAG ATGGACCTAG ATCTTTCCTC 
CTGGATCTCC CAAACTTTCC TGACCTGTCA AAAGCAGATC TCAACGGGCA GAATCCCAAC 
ATTCAGGTTA CCATTGAAGT GGTGGATGGC CCCGACACAG AGACCGACAA GGACCTTCAG 
AAAGAGAGCA GCAAGTCTAG CTGGCCAGTC TCATCACCTG ACTGGAGAAA CTGGTGGCAG 
AGGTCCTCCA CCTTGACTCG CATGAGCAGC GGTGACCAGG ACTACAAGTA TGACAGCACT 
ACTGAGGACA GCAACTTCCT GAACCCTCTT GGTGGGTGGG ATGGTCATGC ACCCAGTCAC 
CGGACATTTG AGTCCAAGGA GCAGCCAGAG TATGACTACA TTGATGGTGA GGGAGACTGG 
AGCCCATGGT CCCTTTGTAG CGTCACCTGT GGAAGCGGCA GTCAGAAGCG GACGAGGTCC 
TGTGGGTATG CCTGTACTGC CACCGAGTCA CGGACCTGCG ACAGGCCAAA CTGCCCAGGG 
ATTGAAGATA CCTTCCGGAC TGCAGCCACA GAAGTGAGCT TACTCGCTGG GAGCGAAGAC 
TTCAATGCTA CCAAACTGTT TGAAGTTGAT ACTGATAGCT GTGAAAGATG GATGAGCTGC 
AAAAGTGAGT TCTTGAAGAA ATACATGCAC AAAGTGGTCA ACGATCTTCC CAGCTGTCCT 
TGCTCCTACC CCAGAGAAGT TGCTTACAGC ACTGCTGACA TCTACGATCG GATTAAGAGG 
AAAAACTTTC GTTGGAAAGA TGCAAGTGGT CCAAAAGAAA AACTGGAGAT CTATAAGCCT 
ACTGCACGGT ACTGCATCCG CTCCATGCTC TCTTTGGAGA GCACAACCCT GGCAGCTCAA 
CACTGCTGCT ATGATGATAA CATGCAGCTG ATTACCAGAG GGAAAGGGGC AGGTACACCA 
AACCTGATCA GCATCGAATT CTCAGCAGAA CTCCATTACA AAGTGGACAT TCTGCCCTGG 
ATTATATGCA AAGGTGACTG GAGCCGGTAC AATGAAGCAC GGCCTCCAAA CAATGGGCAG 
AAGTGTACAG AAAACCCTAC AGATGAAGAC TATTACAAGC AATTTCAAGA AGCAAGGGAA 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139010.1
CDS	11051..13516
Translation	MGGGRQAAAELRRERRERGGASTGEDSRGGRQGTIKASAAHLSRRRARGRESWCGSSSGSGTRADRPPAHTHARWQERVRRCPCPALGDREKGWSQVPSSHRGQPAEPPAVPRPGEPSARRRRKERRRRRRKRRGWGGCPSLPPSRREGPGREPRGALTEGGRDGGSGGAGEDTAAGGQLRGHPHPLARGEPRAPRSLLPSGLPRHVRAAPRLSSLPRTHAYTRARLPAPPGPAARSLRPSDGPRCLWLAGLLAETPPARGPAATCRPPRGSHGIPAAASAASRPDVAPPWCGGPRCCCCCCCRRCPADAESGAVPAADPGAAPAAAAAPAAAAAAPPPPPPPPRPPPPRGPKAARLKRMVRLAAELLLLLGLLLLTLHITVLRGGGGEPQGPAGAANHSQRQNTLTADDHSPEDSFTLNPEDRREYPDLQAAHRPFPKQRFRQENGHTSLQRDGPRSFLLDLPNFPDLSKADLNGQNPNIQVTIEVVDGPDTETDKDLQKESSKSSWPVSSPDWRNWWQRSSTLTRMSSGDQDYKYDSTTEDSNFLNPLGGWDGHAPSHRTFESKEQPEYDYIDGEGDWSPWSLCSVTCGSGSQKRTRSCGYACTATESRTCDRPNCPGIEDTFRTAATEVSLLAGSEDFNATKLFEVDTDSCERWMSCKSEFLKKYMHKVVNDLPSCPCSYPREVAYSTADIYDRIKRKNFRWKDASGPKEKLEIYKPTARYCIRSMLSLESTTLAAQHCCYDDNMQLITRGKGAGTPNLISIEFSAELHYKVDILPWIICKGDWSRYNEARPPNNGQKCTENPTDEDYYKQFQEAREY

Target ORF information:

RefSeq Version	XM_015283524.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus isthmin 1, angiogenesis inhibitor (ISM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015283524.1

ORF Insert Sequence:

ATGGGAGGGG GACGGCAGGC AGCGGCGGAG CTGCGGAGAG AGAGAAGGGA AAGGGGGGGA 
GCGAGCACGG GGGAGGACAG CAGGGGAGGG AGGCAGGGCA CTATAAAAGC ATCCGCCGCA 
CACCTCTCCC GGCGCAGAGC GAGGGGGCGA GAAAGTTGGT GCGGGAGCAG CAGCGGCAGC 
GGGACGAGAG CCGACCGCCC GCCCGCACAC ACGCACGCAC GGTGGCAGGA GAGAGTGCGG 
CGCTGCCCCT GCCCAGCCCT AGGAGACAGA GAGAAGGGAT GGAGCCAGGT ACCCAGCTCC 
CATCGAGGGC AGCCGGCTGA GCCTCCCGCA GTCCCGCGGC CCGGGGAGCC CTCAGCAAGG 
AGGAGGAGGA AGGAGAGGAG GAGGAGGAGG AGGAAGAGGA GGGGGTGGGG GGGCTGCCCC 
TCATTGCCCC CCTCTCGCCG GGAGGGGCCG GGCCGGGAGC CGCGGGGCGC CCTGACGGAG 
GGAGGGAGGG ACGGGGGGAG CGGCGGGGCG GGCGAGGACA CGGCGGCGGG GGGGCAGCTC 
CGGGGCCACC CCCATCCCCT CGCGAGGGGG GAGCCGAGAG CCCCCCGCTC TCTCCTCCCC 
TCGGGGCTTC CCCGCCATGT GAGAGCCGCC CCCCGCCTCT CCTCCCTCCC GCGCACACAC 
GCATACACAC GCGCGCGGCT CCCGGCTCCC CCCGGCCCCG CGGCCCGCTC GCTCCGTCCC 
TCCGACGGCC CACGGTGCCT CTGGCTTGCA GGGCTGCTCG CGGAGACGCC GCCTGCCCGC 
GGCCCCGCCG CTACCTGCCG CCCGCCGCGG GGCAGCCACG GGATTCCCGC CGCCGCCTCC 
GCCGCGTCCC GCCCCGACGT GGCTCCGCCG TGGTGTGGCG GCCCCCGCTG CTGCTGCTGC 
TGCTGCTGCC GCCGCTGCCC CGCCGACGCC GAGAGCGGCG CGGTCCCGGC GGCCGACCCC 
GGGGCTGCTC CGGCGGCGGC GGCGGCTCCG GCGGCGGCGG CCGCGGCTCC TCCTCCTCCT 
CCGCCTCCTC CTCGGCCGCC GCCGCCGCGG GGCCCTAAAG CCGCCCGGCT CAAGAGGATG 
GTGCGGCTGG CGGCGGAGCT GCTGCTGCTG CTGGGGCTGC TCCTGCTCAC CTTGCACATC 
ACCGTGCTGC GCGGCGGCGG CGGAGAGCCG CAGGGCCCCG CGGGCGCCGC CAACCACAGC 
CAGCGGCAGA ACACGCTCAC TGCAGATGAC CATTCTCCTG AAGACAGCTT TACCCTGAAC 
CCTGAGGACA GAAGAGAATA CCCTGATCTC CAGGCTGCCC ACCGGCCATT CCCTAAGCAA 
CGATTCAGGC AGGAAAATGG GCATACATCC TTACAAAGAG ATGGACCTAG ATCTTTCCTC 
CTGGATCTCC CAAACTTTCC TGACCTGTCA AAAGCAGATC TCAACGGGCA GAATCCCAAC 
ATTCAGGTTA CCATTGAAGT GGTGGATGGC CCCGACACAG AGACCGACAA GGACCTTCAG 
AAAGAGAGCA GCAAGTCTAG CTGGCCAGTC TCATCACCTG ACTGGAGAAA CTGGTGGCAG 
AGGTCCTCCA CCTTGACTCG CATGAGCAGC GGTGACCAGG ACTACAAGTA TGACAGCACT 
ACTGAGGACA GCAACTTCCT GAACCCTCTT GGTGGGTGGG ATGGTCATGC ACCCAGTCAC 
CGGACATTTG AGTCCAAGGA GCAGCCAGAG TATGACTACA TTGATGGTGA GGGAGACTGG 
AGCCCATGGT CCCTTTGTAG CGTCACCTGT GGAAGCGGCA GTCAGAAGCG GACGAGGTCC 
TGTGGGTATG CCTGTACTGC CACCGAGTCA CGGACCTGCG ACAGGCCAAA CTGCCCAGGG 
ATTGAAGATA CCTTCCGGAC TGCAGCCACA GAAGTGAGCT TACTCGCTGG GAGCGAAGAC 
TTCAATGCTA CCAAACTGTT TGAAGTTGAT ACTGATAGCT GTGAAAGATG GATGAGCTGC 
AAAAGTGAGT TCTTGAAGAA ATACATGCAC AAAGTGGTCA ACGATCTTCC CAGCTGTCCT 
TGCTCCTACC CCAGAGAAGT TGCTTACAGC ACTGCTGACA TCTACGATCG GATTAAGAGG 
AAAAACTTTC GTTGGAAAGA TGCAAGTGGT CCAAAAGAAA AACTGGAGAT CTATAAGCCT 
ACTGCACGGT ACTGCATCCG CTCCATGCTC TCTTTGGAGA GCACAACCCT GGCAGCTCAA 
CACTGCTGCT ATGATGATAA CATGCAGCTG ATTACCAGAG GGAAAGGGGC AGGTACACCA 
AACCTGATCA GCATCGAATT CTCAGCAGAA CTCCATTACA AAGTGGACAT TCTGCCCTGG 
ATTATATGCA AAGGTGACTG GAGCCGGTAC AATGAAGCAC GGCCTCCAAA CAATGGGCAG 
AAGTGTACAG AAAACCCTAC AGATGAAGAC TATTACAAGC AATTTCAAGA AGCAAGGGAA 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ISM1 ( XM_415036.5 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_415036.5 Gallus gallus isthmin 1, angiogenesis inhibitor (ISM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa33241
Clone ID Related Accession (Same CDS sequence)	XM_015283524.1 , XM_415036.5 , XM_015283524.2 , XM_415036.6
Accession Version	XM_415036.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	isthmin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455832.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_415036.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGGGG GACGGCAGGC AGCGGCGGAG CTGCGGAGAG AGAGAAGGGA AAGGGGGGGA 
GCGAGCACGG GGGAGGACAG CAGGGGAGGG AGGCAGGGCA CTATAAAAGC ATCCGCCGCA 
CACCTCTCCC GGCGCAGAGC GAGGGGGCGA GAAAGTTGGT GCGGGAGCAG CAGCGGCAGC 
GGGACGAGAG CCGACCGCCC GCCCGCACAC ACGCACGCAC GGTGGCAGGA GAGAGTGCGG 
CGCTGCCCCT GCCCAGCCCT AGGAGACAGA GAGAAGGGAT GGAGCCAGGT ACCCAGCTCC 
CATCGAGGGC AGCCGGCTGA GCCTCCCGCA GTCCCGCGGC CCGGGGAGCC CTCAGCAAGG 
AGGAGGAGGA AGGAGAGGAG GAGGAGGAGG AGGAAGAGGA GGGGGTGGGG GGGCTGCCCC 
TCATTGCCCC CCTCTCGCCG GGAGGGGCCG GGCCGGGAGC CGCGGGGCGC CCTGACGGAG 
GGAGGGAGGG ACGGGGGGAG CGGCGGGGCG GGCGAGGACA CGGCGGCGGG GGGGCAGCTC 
CGGGGCCACC CCCATCCCCT CGCGAGGGGG GAGCCGAGAG CCCCCCGCTC TCTCCTCCCC 
TCGGGGCTTC CCCGCCATGT GAGAGCCGCC CCCCGCCTCT CCTCCCTCCC GCGCACACAC 
GCATACACAC GCGCGCGGCT CCCGGCTCCC CCCGGCCCCG CGGCCCGCTC GCTCCGTCCC 
TCCGACGGCC CACGGTGCCT CTGGCTTGCA GGGCTGCTCG CGGAGACGCC GCCTGCCCGC 
GGCCCCGCCG CTACCTGCCG CCCGCCGCGG GGCAGCCACG GGATTCCCGC CGCCGCCTCC 
GCCGCGTCCC GCCCCGACGT GGCTCCGCCG TGGTGTGGCG GCCCCCGCTG CTGCTGCTGC 
TGCTGCTGCC GCCGCTGCCC CGCCGACGCC GAGAGCGGCG CGGTCCCGGC GGCCGACCCC 
GGGGCTGCTC CGGCGGCGGC GGCGGCTCCG GCGGCGGCGG CCGCGGCTCC TCCTCCTCCT 
CCGCCTCCTC CTCGGCCGCC GCCGCCGCGG GGCCCTAAAG CCGCCCGGCT CAAGAGGATG 
GTGCGGCTGG CGGCGGAGCT GCTGCTGCTG CTGGGGCTGC TCCTGCTCAC CTTGCACATC 
ACCGTGCTGC GCGGCGGCGG CGGAGAGCCG CAGGGCCCCG CGGGCGCCGC CAACCACAGC 
CAGCGGCAGA ACACGCTCAC TGCAGATGAC CATTCTCCTG AAGACAGCTT TACCCTGAAC 
CCTGAGGACA GAAGAGAATA CCCTGATCTC CAGGCTGCCC ACCGGCCATT CCCTAAGCAA 
CGATTCAGGC AGGAAAATGG GCATACATCC TTACAAAGAG ATGGACCTAG ATCTTTCCTC 
CTGGATCTCC CAAACTTTCC TGACCTGTCA AAAGCAGATC TCAACGGGCA GAATCCCAAC 
ATTCAGGTTA CCATTGAAGT GGTGGATGGC CCCGACACAG AGACCGACAA GGACCTTCAG 
AAAGAGAGCA GCAAGTCTAG CTGGCCAGTC TCATCACCTG ACTGGAGAAA CTGGTGGCAG 
AGGTCCTCCA CCTTGACTCG CATGAGCAGC GGTGACCAGG ACTACAAGTA TGACAGCACT 
ACTGAGGACA GCAACTTCCT GAACCCTCTT GGTGGGTGGG ATGGTCATGC ACCCAGTCAC 
CGGACATTTG AGTCCAAGGA GCAGCCAGAG TATGACTACA TTGATGGTGA GGGAGACTGG 
AGCCCATGGT CCCTTTGTAG CGTCACCTGT GGAAGCGGCA GTCAGAAGCG GACGAGGTCC 
TGTGGGTATG CCTGTACTGC CACCGAGTCA CGGACCTGCG ACAGGCCAAA CTGCCCAGGG 
ATTGAAGATA CCTTCCGGAC TGCAGCCACA GAAGTGAGCT TACTCGCTGG GAGCGAAGAC 
TTCAATGCTA CCAAACTGTT TGAAGTTGAT ACTGATAGCT GTGAAAGATG GATGAGCTGC 
AAAAGTGAGT TCTTGAAGAA ATACATGCAC AAAGTGGTCA ACGATCTTCC CAGCTGTCCT 
TGCTCCTACC CCAGAGAAGT TGCTTACAGC ACTGCTGACA TCTACGATCG GATTAAGAGG 
AAAAACTTTC GTTGGAAAGA TGCAAGTGGT CCAAAAGAAA AACTGGAGAT CTATAAGCCT 
ACTGCACGGT ACTGCATCCG CTCCATGCTC TCTTTGGAGA GCACAACCCT GGCAGCTCAA 
CACTGCTGCT ATGATGATAA CATGCAGCTG ATTACCAGAG GGAAAGGGGC AGGTACACCA 
AACCTGATCA GCATCGAATT CTCAGCAGAA CTCCATTACA AAGTGGACAT TCTGCCCTGG 
ATTATATGCA AAGGTGACTG GAGCCGGTAC AATGAAGCAC GGCCTCCAAA CAATGGGCAG 
AAGTGTACAG AAAACCCTAC AGATGAAGAC TATTACAAGC AATTTCAAGA AGCAAGGGAA 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_415036.5
CDS	12206..14671
Translation	MGGGRQAAAELRRERRERGGASTGEDSRGGRQGTIKASAAHLSRRRARGRESWCGSSSGSGTRADRPPAHTHARWQERVRRCPCPALGDREKGWSQVPSSHRGQPAEPPAVPRPGEPSARRRRKERRRRRRKRRGWGGCPSLPPSRREGPGREPRGALTEGGRDGGSGGAGEDTAAGGQLRGHPHPLARGEPRAPRSLLPSGLPRHVRAAPRLSSLPRTHAYTRARLPAPPGPAARSLRPSDGPRCLWLAGLLAETPPARGPAATCRPPRGSHGIPAAASAASRPDVAPPWCGGPRCCCCCCCRRCPADAESGAVPAADPGAAPAAAAAPAAAAAAPPPPPPPPRPPPPRGPKAARLKRMVRLAAELLLLLGLLLLTLHITVLRGGGGEPQGPAGAANHSQRQNTLTADDHSPEDSFTLNPEDRREYPDLQAAHRPFPKQRFRQENGHTSLQRDGPRSFLLDLPNFPDLSKADLNGQNPNIQVTIEVVDGPDTETDKDLQKESSKSSWPVSSPDWRNWWQRSSTLTRMSSGDQDYKYDSTTEDSNFLNPLGGWDGHAPSHRTFESKEQPEYDYIDGEGDWSPWSLCSVTCGSGSQKRTRSCGYACTATESRTCDRPNCPGIEDTFRTAATEVSLLAGSEDFNATKLFEVDTDSCERWMSCKSEFLKKYMHKVVNDLPSCPCSYPREVAYSTADIYDRIKRKNFRWKDASGPKEKLEIYKPTARYCIRSMLSLESTTLAAQHCCYDDNMQLITRGKGAGTPNLISIEFSAELHYKVDILPWIICKGDWSRYNEARPPNNGQKCTENPTDEDYYKQFQEAREY

Target ORF information:

RefSeq Version	XM_415036.5
Organism	Gallus gallus(chicken)
Definition	Gallus gallus isthmin 1, angiogenesis inhibitor (ISM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_415036.5

ORF Insert Sequence:

ATGGGAGGGG GACGGCAGGC AGCGGCGGAG CTGCGGAGAG AGAGAAGGGA AAGGGGGGGA 
GCGAGCACGG GGGAGGACAG CAGGGGAGGG AGGCAGGGCA CTATAAAAGC ATCCGCCGCA 
CACCTCTCCC GGCGCAGAGC GAGGGGGCGA GAAAGTTGGT GCGGGAGCAG CAGCGGCAGC 
GGGACGAGAG CCGACCGCCC GCCCGCACAC ACGCACGCAC GGTGGCAGGA GAGAGTGCGG 
CGCTGCCCCT GCCCAGCCCT AGGAGACAGA GAGAAGGGAT GGAGCCAGGT ACCCAGCTCC 
CATCGAGGGC AGCCGGCTGA GCCTCCCGCA GTCCCGCGGC CCGGGGAGCC CTCAGCAAGG 
AGGAGGAGGA AGGAGAGGAG GAGGAGGAGG AGGAAGAGGA GGGGGTGGGG GGGCTGCCCC 
TCATTGCCCC CCTCTCGCCG GGAGGGGCCG GGCCGGGAGC CGCGGGGCGC CCTGACGGAG 
GGAGGGAGGG ACGGGGGGAG CGGCGGGGCG GGCGAGGACA CGGCGGCGGG GGGGCAGCTC 
CGGGGCCACC CCCATCCCCT CGCGAGGGGG GAGCCGAGAG CCCCCCGCTC TCTCCTCCCC 
TCGGGGCTTC CCCGCCATGT GAGAGCCGCC CCCCGCCTCT CCTCCCTCCC GCGCACACAC 
GCATACACAC GCGCGCGGCT CCCGGCTCCC CCCGGCCCCG CGGCCCGCTC GCTCCGTCCC 
TCCGACGGCC CACGGTGCCT CTGGCTTGCA GGGCTGCTCG CGGAGACGCC GCCTGCCCGC 
GGCCCCGCCG CTACCTGCCG CCCGCCGCGG GGCAGCCACG GGATTCCCGC CGCCGCCTCC 
GCCGCGTCCC GCCCCGACGT GGCTCCGCCG TGGTGTGGCG GCCCCCGCTG CTGCTGCTGC 
TGCTGCTGCC GCCGCTGCCC CGCCGACGCC GAGAGCGGCG CGGTCCCGGC GGCCGACCCC 
GGGGCTGCTC CGGCGGCGGC GGCGGCTCCG GCGGCGGCGG CCGCGGCTCC TCCTCCTCCT 
CCGCCTCCTC CTCGGCCGCC GCCGCCGCGG GGCCCTAAAG CCGCCCGGCT CAAGAGGATG 
GTGCGGCTGG CGGCGGAGCT GCTGCTGCTG CTGGGGCTGC TCCTGCTCAC CTTGCACATC 
ACCGTGCTGC GCGGCGGCGG CGGAGAGCCG CAGGGCCCCG CGGGCGCCGC CAACCACAGC 
CAGCGGCAGA ACACGCTCAC TGCAGATGAC CATTCTCCTG AAGACAGCTT TACCCTGAAC 
CCTGAGGACA GAAGAGAATA CCCTGATCTC CAGGCTGCCC ACCGGCCATT CCCTAAGCAA 
CGATTCAGGC AGGAAAATGG GCATACATCC TTACAAAGAG ATGGACCTAG ATCTTTCCTC 
CTGGATCTCC CAAACTTTCC TGACCTGTCA AAAGCAGATC TCAACGGGCA GAATCCCAAC 
ATTCAGGTTA CCATTGAAGT GGTGGATGGC CCCGACACAG AGACCGACAA GGACCTTCAG 
AAAGAGAGCA GCAAGTCTAG CTGGCCAGTC TCATCACCTG ACTGGAGAAA CTGGTGGCAG 
AGGTCCTCCA CCTTGACTCG CATGAGCAGC GGTGACCAGG ACTACAAGTA TGACAGCACT 
ACTGAGGACA GCAACTTCCT GAACCCTCTT GGTGGGTGGG ATGGTCATGC ACCCAGTCAC 
CGGACATTTG AGTCCAAGGA GCAGCCAGAG TATGACTACA TTGATGGTGA GGGAGACTGG 
AGCCCATGGT CCCTTTGTAG CGTCACCTGT GGAAGCGGCA GTCAGAAGCG GACGAGGTCC 
TGTGGGTATG CCTGTACTGC CACCGAGTCA CGGACCTGCG ACAGGCCAAA CTGCCCAGGG 
ATTGAAGATA CCTTCCGGAC TGCAGCCACA GAAGTGAGCT TACTCGCTGG GAGCGAAGAC 
TTCAATGCTA CCAAACTGTT TGAAGTTGAT ACTGATAGCT GTGAAAGATG GATGAGCTGC 
AAAAGTGAGT TCTTGAAGAA ATACATGCAC AAAGTGGTCA ACGATCTTCC CAGCTGTCCT 
TGCTCCTACC CCAGAGAAGT TGCTTACAGC ACTGCTGACA TCTACGATCG GATTAAGAGG 
AAAAACTTTC GTTGGAAAGA TGCAAGTGGT CCAAAAGAAA AACTGGAGAT CTATAAGCCT 
ACTGCACGGT ACTGCATCCG CTCCATGCTC TCTTTGGAGA GCACAACCCT GGCAGCTCAA 
CACTGCTGCT ATGATGATAA CATGCAGCTG ATTACCAGAG GGAAAGGGGC AGGTACACCA 
AACCTGATCA GCATCGAATT CTCAGCAGAA CTCCATTACA AAGTGGACAT TCTGCCCTGG 
ATTATATGCA AAGGTGACTG GAGCCGGTAC AATGAAGCAC GGCCTCCAAA CAATGGGCAG 
AAGTGTACAG AAAACCCTAC AGATGAAGAC TATTACAAGC AATTTCAAGA AGCAAGGGAA 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ISM1 ( XM_015283524.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139010.1 Gallus gallus isthmin 1 (ISM1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa33241
Clone ID Related Accession (Same CDS sequence)	XM_015283524.1 , XM_415036.5 , XM_015283524.2 , XM_415036.6
Accession Version	XM_015283524.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	isthmin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015283524.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGGGG GACGGCAGGC AGCGGCGGAG CTGCGGAGAG AGAGAAGGGA AAGGGGGGGA 
GCGAGCACGG GGGAGGACAG CAGGGGAGGG AGGCAGGGCA CTATAAAAGC ATCCGCCGCA 
CACCTCTCCC GGCGCAGAGC GAGGGGGCGA GAAAGTTGGT GCGGGAGCAG CAGCGGCAGC 
GGGACGAGAG CCGACCGCCC GCCCGCACAC ACGCACGCAC GGTGGCAGGA GAGAGTGCGG 
CGCTGCCCCT GCCCAGCCCT AGGAGACAGA GAGAAGGGAT GGAGCCAGGT ACCCAGCTCC 
CATCGAGGGC AGCCGGCTGA GCCTCCCGCA GTCCCGCGGC CCGGGGAGCC CTCAGCAAGG 
AGGAGGAGGA AGGAGAGGAG GAGGAGGAGG AGGAAGAGGA GGGGGTGGGG GGGCTGCCCC 
TCATTGCCCC CCTCTCGCCG GGAGGGGCCG GGCCGGGAGC CGCGGGGCGC CCTGACGGAG 
GGAGGGAGGG ACGGGGGGAG CGGCGGGGCG GGCGAGGACA CGGCGGCGGG GGGGCAGCTC 
CGGGGCCACC CCCATCCCCT CGCGAGGGGG GAGCCGAGAG CCCCCCGCTC TCTCCTCCCC 
TCGGGGCTTC CCCGCCATGT GAGAGCCGCC CCCCGCCTCT CCTCCCTCCC GCGCACACAC 
GCATACACAC GCGCGCGGCT CCCGGCTCCC CCCGGCCCCG CGGCCCGCTC GCTCCGTCCC 
TCCGACGGCC CACGGTGCCT CTGGCTTGCA GGGCTGCTCG CGGAGACGCC GCCTGCCCGC 
GGCCCCGCCG CTACCTGCCG CCCGCCGCGG GGCAGCCACG GGATTCCCGC CGCCGCCTCC 
GCCGCGTCCC GCCCCGACGT GGCTCCGCCG TGGTGTGGCG GCCCCCGCTG CTGCTGCTGC 
TGCTGCTGCC GCCGCTGCCC CGCCGACGCC GAGAGCGGCG CGGTCCCGGC GGCCGACCCC 
GGGGCTGCTC CGGCGGCGGC GGCGGCTCCG GCGGCGGCGG CCGCGGCTCC TCCTCCTCCT 
CCGCCTCCTC CTCGGCCGCC GCCGCCGCGG GGCCCTAAAG CCGCCCGGCT CAAGAGGATG 
GTGCGGCTGG CGGCGGAGCT GCTGCTGCTG CTGGGGCTGC TCCTGCTCAC CTTGCACATC 
ACCGTGCTGC GCGGCGGCGG CGGAGAGCCG CAGGGCCCCG CGGGCGCCGC CAACCACAGC 
CAGCGGCAGA ACACGCTCAC TGCAGATGAC CATTCTCCTG AAGACAGCTT TACCCTGAAC 
CCTGAGGACA GAAGAGAATA CCCTGATCTC CAGGCTGCCC ACCGGCCATT CCCTAAGCAA 
CGATTCAGGC AGGAAAATGG GCATACATCC TTACAAAGAG ATGGACCTAG ATCTTTCCTC 
CTGGATCTCC CAAACTTTCC TGACCTGTCA AAAGCAGATC TCAACGGGCA GAATCCCAAC 
ATTCAGGTTA CCATTGAAGT GGTGGATGGC CCCGACACAG AGACCGACAA GGACCTTCAG 
AAAGAGAGCA GCAAGTCTAG CTGGCCAGTC TCATCACCTG ACTGGAGAAA CTGGTGGCAG 
AGGTCCTCCA CCTTGACTCG CATGAGCAGC GGTGACCAGG ACTACAAGTA TGACAGCACT 
ACTGAGGACA GCAACTTCCT GAACCCTCTT GGTGGGTGGG ATGGTCATGC ACCCAGTCAC 
CGGACATTTG AGTCCAAGGA GCAGCCAGAG TATGACTACA TTGATGGTGA GGGAGACTGG 
AGCCCATGGT CCCTTTGTAG CGTCACCTGT GGAAGCGGCA GTCAGAAGCG GACGAGGTCC 
TGTGGGTATG CCTGTACTGC CACCGAGTCA CGGACCTGCG ACAGGCCAAA CTGCCCAGGG 
ATTGAAGATA CCTTCCGGAC TGCAGCCACA GAAGTGAGCT TACTCGCTGG GAGCGAAGAC 
TTCAATGCTA CCAAACTGTT TGAAGTTGAT ACTGATAGCT GTGAAAGATG GATGAGCTGC 
AAAAGTGAGT TCTTGAAGAA ATACATGCAC AAAGTGGTCA ACGATCTTCC CAGCTGTCCT 
TGCTCCTACC CCAGAGAAGT TGCTTACAGC ACTGCTGACA TCTACGATCG GATTAAGAGG 
AAAAACTTTC GTTGGAAAGA TGCAAGTGGT CCAAAAGAAA AACTGGAGAT CTATAAGCCT 
ACTGCACGGT ACTGCATCCG CTCCATGCTC TCTTTGGAGA GCACAACCCT GGCAGCTCAA 
CACTGCTGCT ATGATGATAA CATGCAGCTG ATTACCAGAG GGAAAGGGGC AGGTACACCA 
AACCTGATCA GCATCGAATT CTCAGCAGAA CTCCATTACA AAGTGGACAT TCTGCCCTGG 
ATTATATGCA AAGGTGACTG GAGCCGGTAC AATGAAGCAC GGCCTCCAAA CAATGGGCAG 
AAGTGTACAG AAAACCCTAC AGATGAAGAC TATTACAAGC AATTTCAAGA AGCAAGGGAA 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139010.1
CDS	11478..13943
Translation	MGGGRQAAAELRRERRERGGASTGEDSRGGRQGTIKASAAHLSRRRARGRESWCGSSSGSGTRADRPPAHTHARWQERVRRCPCPALGDREKGWSQVPSSHRGQPAEPPAVPRPGEPSARRRRKERRRRRRKRRGWGGCPSLPPSRREGPGREPRGALTEGGRDGGSGGAGEDTAAGGQLRGHPHPLARGEPRAPRSLLPSGLPRHVRAAPRLSSLPRTHAYTRARLPAPPGPAARSLRPSDGPRCLWLAGLLAETPPARGPAATCRPPRGSHGIPAAASAASRPDVAPPWCGGPRCCCCCCCRRCPADAESGAVPAADPGAAPAAAAAPAAAAAAPPPPPPPPRPPPPRGPKAARLKRMVRLAAELLLLLGLLLLTLHITVLRGGGGEPQGPAGAANHSQRQNTLTADDHSPEDSFTLNPEDRREYPDLQAAHRPFPKQRFRQENGHTSLQRDGPRSFLLDLPNFPDLSKADLNGQNPNIQVTIEVVDGPDTETDKDLQKESSKSSWPVSSPDWRNWWQRSSTLTRMSSGDQDYKYDSTTEDSNFLNPLGGWDGHAPSHRTFESKEQPEYDYIDGEGDWSPWSLCSVTCGSGSQKRTRSCGYACTATESRTCDRPNCPGIEDTFRTAATEVSLLAGSEDFNATKLFEVDTDSCERWMSCKSEFLKKYMHKVVNDLPSCPCSYPREVAYSTADIYDRIKRKNFRWKDASGPKEKLEIYKPTARYCIRSMLSLESTTLAAQHCCYDDNMQLITRGKGAGTPNLISIEFSAELHYKVDILPWIICKGDWSRYNEARPPNNGQKCTENPTDEDYYKQFQEAREY

Target ORF information:

RefSeq Version	XM_015283524.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus isthmin 1 (ISM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015283524.2

ORF Insert Sequence:

ATGGGAGGGG GACGGCAGGC AGCGGCGGAG CTGCGGAGAG AGAGAAGGGA AAGGGGGGGA 
GCGAGCACGG GGGAGGACAG CAGGGGAGGG AGGCAGGGCA CTATAAAAGC ATCCGCCGCA 
CACCTCTCCC GGCGCAGAGC GAGGGGGCGA GAAAGTTGGT GCGGGAGCAG CAGCGGCAGC 
GGGACGAGAG CCGACCGCCC GCCCGCACAC ACGCACGCAC GGTGGCAGGA GAGAGTGCGG 
CGCTGCCCCT GCCCAGCCCT AGGAGACAGA GAGAAGGGAT GGAGCCAGGT ACCCAGCTCC 
CATCGAGGGC AGCCGGCTGA GCCTCCCGCA GTCCCGCGGC CCGGGGAGCC CTCAGCAAGG 
AGGAGGAGGA AGGAGAGGAG GAGGAGGAGG AGGAAGAGGA GGGGGTGGGG GGGCTGCCCC 
TCATTGCCCC CCTCTCGCCG GGAGGGGCCG GGCCGGGAGC CGCGGGGCGC CCTGACGGAG 
GGAGGGAGGG ACGGGGGGAG CGGCGGGGCG GGCGAGGACA CGGCGGCGGG GGGGCAGCTC 
CGGGGCCACC CCCATCCCCT CGCGAGGGGG GAGCCGAGAG CCCCCCGCTC TCTCCTCCCC 
TCGGGGCTTC CCCGCCATGT GAGAGCCGCC CCCCGCCTCT CCTCCCTCCC GCGCACACAC 
GCATACACAC GCGCGCGGCT CCCGGCTCCC CCCGGCCCCG CGGCCCGCTC GCTCCGTCCC 
TCCGACGGCC CACGGTGCCT CTGGCTTGCA GGGCTGCTCG CGGAGACGCC GCCTGCCCGC 
GGCCCCGCCG CTACCTGCCG CCCGCCGCGG GGCAGCCACG GGATTCCCGC CGCCGCCTCC 
GCCGCGTCCC GCCCCGACGT GGCTCCGCCG TGGTGTGGCG GCCCCCGCTG CTGCTGCTGC 
TGCTGCTGCC GCCGCTGCCC CGCCGACGCC GAGAGCGGCG CGGTCCCGGC GGCCGACCCC 
GGGGCTGCTC CGGCGGCGGC GGCGGCTCCG GCGGCGGCGG CCGCGGCTCC TCCTCCTCCT 
CCGCCTCCTC CTCGGCCGCC GCCGCCGCGG GGCCCTAAAG CCGCCCGGCT CAAGAGGATG 
GTGCGGCTGG CGGCGGAGCT GCTGCTGCTG CTGGGGCTGC TCCTGCTCAC CTTGCACATC 
ACCGTGCTGC GCGGCGGCGG CGGAGAGCCG CAGGGCCCCG CGGGCGCCGC CAACCACAGC 
CAGCGGCAGA ACACGCTCAC TGCAGATGAC CATTCTCCTG AAGACAGCTT TACCCTGAAC 
CCTGAGGACA GAAGAGAATA CCCTGATCTC CAGGCTGCCC ACCGGCCATT CCCTAAGCAA 
CGATTCAGGC AGGAAAATGG GCATACATCC TTACAAAGAG ATGGACCTAG ATCTTTCCTC 
CTGGATCTCC CAAACTTTCC TGACCTGTCA AAAGCAGATC TCAACGGGCA GAATCCCAAC 
ATTCAGGTTA CCATTGAAGT GGTGGATGGC CCCGACACAG AGACCGACAA GGACCTTCAG 
AAAGAGAGCA GCAAGTCTAG CTGGCCAGTC TCATCACCTG ACTGGAGAAA CTGGTGGCAG 
AGGTCCTCCA CCTTGACTCG CATGAGCAGC GGTGACCAGG ACTACAAGTA TGACAGCACT 
ACTGAGGACA GCAACTTCCT GAACCCTCTT GGTGGGTGGG ATGGTCATGC ACCCAGTCAC 
CGGACATTTG AGTCCAAGGA GCAGCCAGAG TATGACTACA TTGATGGTGA GGGAGACTGG 
AGCCCATGGT CCCTTTGTAG CGTCACCTGT GGAAGCGGCA GTCAGAAGCG GACGAGGTCC 
TGTGGGTATG CCTGTACTGC CACCGAGTCA CGGACCTGCG ACAGGCCAAA CTGCCCAGGG 
ATTGAAGATA CCTTCCGGAC TGCAGCCACA GAAGTGAGCT TACTCGCTGG GAGCGAAGAC 
TTCAATGCTA CCAAACTGTT TGAAGTTGAT ACTGATAGCT GTGAAAGATG GATGAGCTGC 
AAAAGTGAGT TCTTGAAGAA ATACATGCAC AAAGTGGTCA ACGATCTTCC CAGCTGTCCT 
TGCTCCTACC CCAGAGAAGT TGCTTACAGC ACTGCTGACA TCTACGATCG GATTAAGAGG 
AAAAACTTTC GTTGGAAAGA TGCAAGTGGT CCAAAAGAAA AACTGGAGAT CTATAAGCCT 
ACTGCACGGT ACTGCATCCG CTCCATGCTC TCTTTGGAGA GCACAACCCT GGCAGCTCAA 
CACTGCTGCT ATGATGATAA CATGCAGCTG ATTACCAGAG GGAAAGGGGC AGGTACACCA 
AACCTGATCA GCATCGAATT CTCAGCAGAA CTCCATTACA AAGTGGACAT TCTGCCCTGG 
ATTATATGCA AAGGTGACTG GAGCCGGTAC AATGAAGCAC GGCCTCCAAA CAATGGGCAG 
AAGTGTACAG AAAACCCTAC AGATGAAGAC TATTACAAGC AATTTCAAGA AGCAAGGGAA 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ISM1 ( XM_415036.6 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_415036.5 Gallus gallus isthmin 1 (ISM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa33241
Clone ID Related Accession (Same CDS sequence)	XM_015283524.1 , XM_415036.5 , XM_015283524.2 , XM_415036.6
Accession Version	XM_415036.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	isthmin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_415036.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGGGG GACGGCAGGC AGCGGCGGAG CTGCGGAGAG AGAGAAGGGA AAGGGGGGGA 
GCGAGCACGG GGGAGGACAG CAGGGGAGGG AGGCAGGGCA CTATAAAAGC ATCCGCCGCA 
CACCTCTCCC GGCGCAGAGC GAGGGGGCGA GAAAGTTGGT GCGGGAGCAG CAGCGGCAGC 
GGGACGAGAG CCGACCGCCC GCCCGCACAC ACGCACGCAC GGTGGCAGGA GAGAGTGCGG 
CGCTGCCCCT GCCCAGCCCT AGGAGACAGA GAGAAGGGAT GGAGCCAGGT ACCCAGCTCC 
CATCGAGGGC AGCCGGCTGA GCCTCCCGCA GTCCCGCGGC CCGGGGAGCC CTCAGCAAGG 
AGGAGGAGGA AGGAGAGGAG GAGGAGGAGG AGGAAGAGGA GGGGGTGGGG GGGCTGCCCC 
TCATTGCCCC CCTCTCGCCG GGAGGGGCCG GGCCGGGAGC CGCGGGGCGC CCTGACGGAG 
GGAGGGAGGG ACGGGGGGAG CGGCGGGGCG GGCGAGGACA CGGCGGCGGG GGGGCAGCTC 
CGGGGCCACC CCCATCCCCT CGCGAGGGGG GAGCCGAGAG CCCCCCGCTC TCTCCTCCCC 
TCGGGGCTTC CCCGCCATGT GAGAGCCGCC CCCCGCCTCT CCTCCCTCCC GCGCACACAC 
GCATACACAC GCGCGCGGCT CCCGGCTCCC CCCGGCCCCG CGGCCCGCTC GCTCCGTCCC 
TCCGACGGCC CACGGTGCCT CTGGCTTGCA GGGCTGCTCG CGGAGACGCC GCCTGCCCGC 
GGCCCCGCCG CTACCTGCCG CCCGCCGCGG GGCAGCCACG GGATTCCCGC CGCCGCCTCC 
GCCGCGTCCC GCCCCGACGT GGCTCCGCCG TGGTGTGGCG GCCCCCGCTG CTGCTGCTGC 
TGCTGCTGCC GCCGCTGCCC CGCCGACGCC GAGAGCGGCG CGGTCCCGGC GGCCGACCCC 
GGGGCTGCTC CGGCGGCGGC GGCGGCTCCG GCGGCGGCGG CCGCGGCTCC TCCTCCTCCT 
CCGCCTCCTC CTCGGCCGCC GCCGCCGCGG GGCCCTAAAG CCGCCCGGCT CAAGAGGATG 
GTGCGGCTGG CGGCGGAGCT GCTGCTGCTG CTGGGGCTGC TCCTGCTCAC CTTGCACATC 
ACCGTGCTGC GCGGCGGCGG CGGAGAGCCG CAGGGCCCCG CGGGCGCCGC CAACCACAGC 
CAGCGGCAGA ACACGCTCAC TGCAGATGAC CATTCTCCTG AAGACAGCTT TACCCTGAAC 
CCTGAGGACA GAAGAGAATA CCCTGATCTC CAGGCTGCCC ACCGGCCATT CCCTAAGCAA 
CGATTCAGGC AGGAAAATGG GCATACATCC TTACAAAGAG ATGGACCTAG ATCTTTCCTC 
CTGGATCTCC CAAACTTTCC TGACCTGTCA AAAGCAGATC TCAACGGGCA GAATCCCAAC 
ATTCAGGTTA CCATTGAAGT GGTGGATGGC CCCGACACAG AGACCGACAA GGACCTTCAG 
AAAGAGAGCA GCAAGTCTAG CTGGCCAGTC TCATCACCTG ACTGGAGAAA CTGGTGGCAG 
AGGTCCTCCA CCTTGACTCG CATGAGCAGC GGTGACCAGG ACTACAAGTA TGACAGCACT 
ACTGAGGACA GCAACTTCCT GAACCCTCTT GGTGGGTGGG ATGGTCATGC ACCCAGTCAC 
CGGACATTTG AGTCCAAGGA GCAGCCAGAG TATGACTACA TTGATGGTGA GGGAGACTGG 
AGCCCATGGT CCCTTTGTAG CGTCACCTGT GGAAGCGGCA GTCAGAAGCG GACGAGGTCC 
TGTGGGTATG CCTGTACTGC CACCGAGTCA CGGACCTGCG ACAGGCCAAA CTGCCCAGGG 
ATTGAAGATA CCTTCCGGAC TGCAGCCACA GAAGTGAGCT TACTCGCTGG GAGCGAAGAC 
TTCAATGCTA CCAAACTGTT TGAAGTTGAT ACTGATAGCT GTGAAAGATG GATGAGCTGC 
AAAAGTGAGT TCTTGAAGAA ATACATGCAC AAAGTGGTCA ACGATCTTCC CAGCTGTCCT 
TGCTCCTACC CCAGAGAAGT TGCTTACAGC ACTGCTGACA TCTACGATCG GATTAAGAGG 
AAAAACTTTC GTTGGAAAGA TGCAAGTGGT CCAAAAGAAA AACTGGAGAT CTATAAGCCT 
ACTGCACGGT ACTGCATCCG CTCCATGCTC TCTTTGGAGA GCACAACCCT GGCAGCTCAA 
CACTGCTGCT ATGATGATAA CATGCAGCTG ATTACCAGAG GGAAAGGGGC AGGTACACCA 
AACCTGATCA GCATCGAATT CTCAGCAGAA CTCCATTACA AAGTGGACAT TCTGCCCTGG 
ATTATATGCA AAGGTGACTG GAGCCGGTAC AATGAAGCAC GGCCTCCAAA CAATGGGCAG 
AAGTGTACAG AAAACCCTAC AGATGAAGAC TATTACAAGC AATTTCAAGA AGCAAGGGAA 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_415036.5
CDS	15023..17488
Translation	MGGGRQAAAELRRERRERGGASTGEDSRGGRQGTIKASAAHLSRRRARGRESWCGSSSGSGTRADRPPAHTHARWQERVRRCPCPALGDREKGWSQVPSSHRGQPAEPPAVPRPGEPSARRRRKERRRRRRKRRGWGGCPSLPPSRREGPGREPRGALTEGGRDGGSGGAGEDTAAGGQLRGHPHPLARGEPRAPRSLLPSGLPRHVRAAPRLSSLPRTHAYTRARLPAPPGPAARSLRPSDGPRCLWLAGLLAETPPARGPAATCRPPRGSHGIPAAASAASRPDVAPPWCGGPRCCCCCCCRRCPADAESGAVPAADPGAAPAAAAAPAAAAAAPPPPPPPPRPPPPRGPKAARLKRMVRLAAELLLLLGLLLLTLHITVLRGGGGEPQGPAGAANHSQRQNTLTADDHSPEDSFTLNPEDRREYPDLQAAHRPFPKQRFRQENGHTSLQRDGPRSFLLDLPNFPDLSKADLNGQNPNIQVTIEVVDGPDTETDKDLQKESSKSSWPVSSPDWRNWWQRSSTLTRMSSGDQDYKYDSTTEDSNFLNPLGGWDGHAPSHRTFESKEQPEYDYIDGEGDWSPWSLCSVTCGSGSQKRTRSCGYACTATESRTCDRPNCPGIEDTFRTAATEVSLLAGSEDFNATKLFEVDTDSCERWMSCKSEFLKKYMHKVVNDLPSCPCSYPREVAYSTADIYDRIKRKNFRWKDASGPKEKLEIYKPTARYCIRSMLSLESTTLAAQHCCYDDNMQLITRGKGAGTPNLISIEFSAELHYKVDILPWIICKGDWSRYNEARPPNNGQKCTENPTDEDYYKQFQEAREY

Target ORF information:

RefSeq Version	XM_415036.6
Organism	Gallus gallus(chicken)
Definition	Gallus gallus isthmin 1 (ISM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_415036.6

ORF Insert Sequence:

ATGGGAGGGG GACGGCAGGC AGCGGCGGAG CTGCGGAGAG AGAGAAGGGA AAGGGGGGGA 
GCGAGCACGG GGGAGGACAG CAGGGGAGGG AGGCAGGGCA CTATAAAAGC ATCCGCCGCA 
CACCTCTCCC GGCGCAGAGC GAGGGGGCGA GAAAGTTGGT GCGGGAGCAG CAGCGGCAGC 
GGGACGAGAG CCGACCGCCC GCCCGCACAC ACGCACGCAC GGTGGCAGGA GAGAGTGCGG 
CGCTGCCCCT GCCCAGCCCT AGGAGACAGA GAGAAGGGAT GGAGCCAGGT ACCCAGCTCC 
CATCGAGGGC AGCCGGCTGA GCCTCCCGCA GTCCCGCGGC CCGGGGAGCC CTCAGCAAGG 
AGGAGGAGGA AGGAGAGGAG GAGGAGGAGG AGGAAGAGGA GGGGGTGGGG GGGCTGCCCC 
TCATTGCCCC CCTCTCGCCG GGAGGGGCCG GGCCGGGAGC CGCGGGGCGC CCTGACGGAG 
GGAGGGAGGG ACGGGGGGAG CGGCGGGGCG GGCGAGGACA CGGCGGCGGG GGGGCAGCTC 
CGGGGCCACC CCCATCCCCT CGCGAGGGGG GAGCCGAGAG CCCCCCGCTC TCTCCTCCCC 
TCGGGGCTTC CCCGCCATGT GAGAGCCGCC CCCCGCCTCT CCTCCCTCCC GCGCACACAC 
GCATACACAC GCGCGCGGCT CCCGGCTCCC CCCGGCCCCG CGGCCCGCTC GCTCCGTCCC 
TCCGACGGCC CACGGTGCCT CTGGCTTGCA GGGCTGCTCG CGGAGACGCC GCCTGCCCGC 
GGCCCCGCCG CTACCTGCCG CCCGCCGCGG GGCAGCCACG GGATTCCCGC CGCCGCCTCC 
GCCGCGTCCC GCCCCGACGT GGCTCCGCCG TGGTGTGGCG GCCCCCGCTG CTGCTGCTGC 
TGCTGCTGCC GCCGCTGCCC CGCCGACGCC GAGAGCGGCG CGGTCCCGGC GGCCGACCCC 
GGGGCTGCTC CGGCGGCGGC GGCGGCTCCG GCGGCGGCGG CCGCGGCTCC TCCTCCTCCT 
CCGCCTCCTC CTCGGCCGCC GCCGCCGCGG GGCCCTAAAG CCGCCCGGCT CAAGAGGATG 
GTGCGGCTGG CGGCGGAGCT GCTGCTGCTG CTGGGGCTGC TCCTGCTCAC CTTGCACATC 
ACCGTGCTGC GCGGCGGCGG CGGAGAGCCG CAGGGCCCCG CGGGCGCCGC CAACCACAGC 
CAGCGGCAGA ACACGCTCAC TGCAGATGAC CATTCTCCTG AAGACAGCTT TACCCTGAAC 
CCTGAGGACA GAAGAGAATA CCCTGATCTC CAGGCTGCCC ACCGGCCATT CCCTAAGCAA 
CGATTCAGGC AGGAAAATGG GCATACATCC TTACAAAGAG ATGGACCTAG ATCTTTCCTC 
CTGGATCTCC CAAACTTTCC TGACCTGTCA AAAGCAGATC TCAACGGGCA GAATCCCAAC 
ATTCAGGTTA CCATTGAAGT GGTGGATGGC CCCGACACAG AGACCGACAA GGACCTTCAG 
AAAGAGAGCA GCAAGTCTAG CTGGCCAGTC TCATCACCTG ACTGGAGAAA CTGGTGGCAG 
AGGTCCTCCA CCTTGACTCG CATGAGCAGC GGTGACCAGG ACTACAAGTA TGACAGCACT 
ACTGAGGACA GCAACTTCCT GAACCCTCTT GGTGGGTGGG ATGGTCATGC ACCCAGTCAC 
CGGACATTTG AGTCCAAGGA GCAGCCAGAG TATGACTACA TTGATGGTGA GGGAGACTGG 
AGCCCATGGT CCCTTTGTAG CGTCACCTGT GGAAGCGGCA GTCAGAAGCG GACGAGGTCC 
TGTGGGTATG CCTGTACTGC CACCGAGTCA CGGACCTGCG ACAGGCCAAA CTGCCCAGGG 
ATTGAAGATA CCTTCCGGAC TGCAGCCACA GAAGTGAGCT TACTCGCTGG GAGCGAAGAC 
TTCAATGCTA CCAAACTGTT TGAAGTTGAT ACTGATAGCT GTGAAAGATG GATGAGCTGC 
AAAAGTGAGT TCTTGAAGAA ATACATGCAC AAAGTGGTCA ACGATCTTCC CAGCTGTCCT 
TGCTCCTACC CCAGAGAAGT TGCTTACAGC ACTGCTGACA TCTACGATCG GATTAAGAGG 
AAAAACTTTC GTTGGAAAGA TGCAAGTGGT CCAAAAGAAA AACTGGAGAT CTATAAGCCT 
ACTGCACGGT ACTGCATCCG CTCCATGCTC TCTTTGGAGA GCACAACCCT GGCAGCTCAA 
CACTGCTGCT ATGATGATAA CATGCAGCTG ATTACCAGAG GGAAAGGGGC AGGTACACCA 
AACCTGATCA GCATCGAATT CTCAGCAGAA CTCCATTACA AAGTGGACAT TCTGCCCTGG 
ATTATATGCA AAGGTGACTG GAGCCGGTAC AATGAAGCAC GGCCTCCAAA CAATGGGCAG 
AAGTGTACAG AAAACCCTAC AGATGAAGAC TATTACAAGC AATTTCAAGA AGCAAGGGAA 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ISM1 ( XM_015283525.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139011.1 Gallus gallus isthmin 1 (ISM1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa33242
Clone ID Related Accession (Same CDS sequence)	XM_015283525.2 , XM_015283525.1
Accession Version	XM_015283525.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	isthmin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015283525.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCAG GGCTGCTCGC GGAGACGCCG CCTGCCCGCG GCCCCGCCGC TACCTGCCGC 
CCGCCGCGGG GCAGCCACGG GATTCCCGCC GCCGCCTCCG CCGCGTCCCG CCCCGACGTG 
GCTCCGCCGT GGTGTGGCGG CCCCCGCTGC TGCTGCTGCT GCTGCTGCCG CCGCTGCCCC 
GCCGACGCCG AGAGCGGCGC GGTCCCGGCG GCCGACCCCG GGGCTGCTCC GGCGGCGGCG 
GCGGCTCCGG CGGCGGCGGC CGCGGCTCCT CCTCCTCCTC CGCCTCCTCC TCGGCCGCCG 
CCGCCGCGGG GCCCTAAAGC CGCCCGGCTC AAGAGGATGG TGCGGCTGGC GGCGGAGCTG 
CTGCTGCTGC TGGGGCTGCT CCTGCTCACC TTGCACATCA CCGTGCTGCG CGGCGGCGGC 
GGAGAGCCGC AGGGCCCCGC GGGCGCCGCC AACCACAGCC AGCGGCAGAA CACGCTCACT 
GCAGATGACC ATTCTCCTGA AGACAGCTTT ACCCTGAACC CTGAGGACAG AAGAGAATAC 
CCTGATCTCC AGGCTGCCCA CCGGCCATTC CCTAAGCAAC GATTCAGGCA GGAAAATGGG 
CATACATCCT TACAAAGAGA TGGACCTAGA TCTTTCCTCC TGGATCTCCC AAACTTTCCT 
GACCTGTCAA AAGCAGATCT CAACGGGCAG AATCCCAACA TTCAGGTTAC CATTGAAGTG 
GTGGATGGCC CCGACACAGA GACCGACAAG GACCTTCAGA AAGAGAGCAG CAAGTCTAGC 
TGGCCAGTCT CATCACCTGA CTGGAGAAAC TGGTGGCAGA GGTCCTCCAC CTTGACTCGC 
ATGAGCAGCG GTGACCAGGA CTACAAGTAT GACAGCACTA CTGAGGACAG CAACTTCCTG 
AACCCTCTTG GTGGGTGGGA TGGTCATGCA CCCAGTCACC GGACATTTGA GTCCAAGGAG 
CAGCCAGAGT ATGACTACAT TGATGGTGAG GGAGACTGGA GCCCATGGTC CCTTTGTAGC 
GTCACCTGTG GAAGCGGCAG TCAGAAGCGG ACGAGGTCCT GTGGGTATGC CTGTACTGCC 
ACCGAGTCAC GGACCTGCGA CAGGCCAAAC TGCCCAGGGA TTGAAGATAC CTTCCGGACT 
GCAGCCACAG AAGTGAGCTT ACTCGCTGGG AGCGAAGACT TCAATGCTAC CAAACTGTTT 
GAAGTTGATA CTGATAGCTG TGAAAGATGG ATGAGCTGCA AAAGTGAGTT CTTGAAGAAA 
TACATGCACA AAGTGGTCAA CGATCTTCCC AGCTGTCCTT GCTCCTACCC CAGAGAAGTT 
GCTTACAGCA CTGCTGACAT CTACGATCGG ATTAAGAGGA AAAACTTTCG TTGGAAAGAT 
GCAAGTGGTC CAAAAGAAAA ACTGGAGATC TATAAGCCTA CTGCACGGTA CTGCATCCGC 
TCCATGCTCT CTTTGGAGAG CACAACCCTG GCAGCTCAAC ACTGCTGCTA TGATGATAAC 
ATGCAGCTGA TTACCAGAGG GAAAGGGGCA GGTACACCAA ACCTGATCAG CATCGAATTC 
TCAGCAGAAC TCCATTACAA AGTGGACATT CTGCCCTGGA TTATATGCAA AGGTGACTGG 
AGCCGGTACA ATGAAGCACG GCCTCCAAAC AATGGGCAGA AGTGTACAGA AAACCCTACA 
GATGAAGACT ATTACAAGCA ATTTCAAGAA GCAAGGGAAT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139011.1
CDS	132..1856
Translation	MEPGLLAETPPARGPAATCRPPRGSHGIPAAASAASRPDVAPPWCGGPRCCCCCCCRRCPADAESGAVPAADPGAAPAAAAAPAAAAAAPPPPPPPPRPPPPRGPKAARLKRMVRLAAELLLLLGLLLLTLHITVLRGGGGEPQGPAGAANHSQRQNTLTADDHSPEDSFTLNPEDRREYPDLQAAHRPFPKQRFRQENGHTSLQRDGPRSFLLDLPNFPDLSKADLNGQNPNIQVTIEVVDGPDTETDKDLQKESSKSSWPVSSPDWRNWWQRSSTLTRMSSGDQDYKYDSTTEDSNFLNPLGGWDGHAPSHRTFESKEQPEYDYIDGEGDWSPWSLCSVTCGSGSQKRTRSCGYACTATESRTCDRPNCPGIEDTFRTAATEVSLLAGSEDFNATKLFEVDTDSCERWMSCKSEFLKKYMHKVVNDLPSCPCSYPREVAYSTADIYDRIKRKNFRWKDASGPKEKLEIYKPTARYCIRSMLSLESTTLAAQHCCYDDNMQLITRGKGAGTPNLISIEFSAELHYKVDILPWIICKGDWSRYNEARPPNNGQKCTENPTDEDYYKQFQEAREY

Target ORF information:

RefSeq Version	XM_015283525.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus isthmin 1 (ISM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015283525.2

ORF Insert Sequence:

ATGGAGCCAG GGCTGCTCGC GGAGACGCCG CCTGCCCGCG GCCCCGCCGC TACCTGCCGC 
CCGCCGCGGG GCAGCCACGG GATTCCCGCC GCCGCCTCCG CCGCGTCCCG CCCCGACGTG 
GCTCCGCCGT GGTGTGGCGG CCCCCGCTGC TGCTGCTGCT GCTGCTGCCG CCGCTGCCCC 
GCCGACGCCG AGAGCGGCGC GGTCCCGGCG GCCGACCCCG GGGCTGCTCC GGCGGCGGCG 
GCGGCTCCGG CGGCGGCGGC CGCGGCTCCT CCTCCTCCTC CGCCTCCTCC TCGGCCGCCG 
CCGCCGCGGG GCCCTAAAGC CGCCCGGCTC AAGAGGATGG TGCGGCTGGC GGCGGAGCTG 
CTGCTGCTGC TGGGGCTGCT CCTGCTCACC TTGCACATCA CCGTGCTGCG CGGCGGCGGC 
GGAGAGCCGC AGGGCCCCGC GGGCGCCGCC AACCACAGCC AGCGGCAGAA CACGCTCACT 
GCAGATGACC ATTCTCCTGA AGACAGCTTT ACCCTGAACC CTGAGGACAG AAGAGAATAC 
CCTGATCTCC AGGCTGCCCA CCGGCCATTC CCTAAGCAAC GATTCAGGCA GGAAAATGGG 
CATACATCCT TACAAAGAGA TGGACCTAGA TCTTTCCTCC TGGATCTCCC AAACTTTCCT 
GACCTGTCAA AAGCAGATCT CAACGGGCAG AATCCCAACA TTCAGGTTAC CATTGAAGTG 
GTGGATGGCC CCGACACAGA GACCGACAAG GACCTTCAGA AAGAGAGCAG CAAGTCTAGC 
TGGCCAGTCT CATCACCTGA CTGGAGAAAC TGGTGGCAGA GGTCCTCCAC CTTGACTCGC 
ATGAGCAGCG GTGACCAGGA CTACAAGTAT GACAGCACTA CTGAGGACAG CAACTTCCTG 
AACCCTCTTG GTGGGTGGGA TGGTCATGCA CCCAGTCACC GGACATTTGA GTCCAAGGAG 
CAGCCAGAGT ATGACTACAT TGATGGTGAG GGAGACTGGA GCCCATGGTC CCTTTGTAGC 
GTCACCTGTG GAAGCGGCAG TCAGAAGCGG ACGAGGTCCT GTGGGTATGC CTGTACTGCC 
ACCGAGTCAC GGACCTGCGA CAGGCCAAAC TGCCCAGGGA TTGAAGATAC CTTCCGGACT 
GCAGCCACAG AAGTGAGCTT ACTCGCTGGG AGCGAAGACT TCAATGCTAC CAAACTGTTT 
GAAGTTGATA CTGATAGCTG TGAAAGATGG ATGAGCTGCA AAAGTGAGTT CTTGAAGAAA 
TACATGCACA AAGTGGTCAA CGATCTTCCC AGCTGTCCTT GCTCCTACCC CAGAGAAGTT 
GCTTACAGCA CTGCTGACAT CTACGATCGG ATTAAGAGGA AAAACTTTCG TTGGAAAGAT 
GCAAGTGGTC CAAAAGAAAA ACTGGAGATC TATAAGCCTA CTGCACGGTA CTGCATCCGC 
TCCATGCTCT CTTTGGAGAG CACAACCCTG GCAGCTCAAC ACTGCTGCTA TGATGATAAC 
ATGCAGCTGA TTACCAGAGG GAAAGGGGCA GGTACACCAA ACCTGATCAG CATCGAATTC 
TCAGCAGAAC TCCATTACAA AGTGGACATT CTGCCCTGGA TTATATGCAA AGGTGACTGG 
AGCCGGTACA ATGAAGCACG GCCTCCAAAC AATGGGCAGA AGTGTACAGA AAACCCTACA 
GATGAAGACT ATTACAAGCA ATTTCAAGAA GCAAGGGAAT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ISM1 ( XM_015283525.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015139011.1 Gallus gallus isthmin 1, angiogenesis inhibitor (ISM1), transcript variant X3, mRNA.

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CloneID	OGa33242
Clone ID Related Accession (Same CDS sequence)	XM_015283525.2 , XM_015283525.1
Accession Version	XM_015283525.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	isthmin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455832.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCAG GGCTGCTCGC GGAGACGCCG CCTGCCCGCG GCCCCGCCGC TACCTGCCGC 
CCGCCGCGGG GCAGCCACGG GATTCCCGCC GCCGCCTCCG CCGCGTCCCG CCCCGACGTG 
GCTCCGCCGT GGTGTGGCGG CCCCCGCTGC TGCTGCTGCT GCTGCTGCCG CCGCTGCCCC 
GCCGACGCCG AGAGCGGCGC GGTCCCGGCG GCCGACCCCG GGGCTGCTCC GGCGGCGGCG 
GCGGCTCCGG CGGCGGCGGC CGCGGCTCCT CCTCCTCCTC CGCCTCCTCC TCGGCCGCCG 
CCGCCGCGGG GCCCTAAAGC CGCCCGGCTC AAGAGGATGG TGCGGCTGGC GGCGGAGCTG 
CTGCTGCTGC TGGGGCTGCT CCTGCTCACC TTGCACATCA CCGTGCTGCG CGGCGGCGGC 
GGAGAGCCGC AGGGCCCCGC GGGCGCCGCC AACCACAGCC AGCGGCAGAA CACGCTCACT 
GCAGATGACC ATTCTCCTGA AGACAGCTTT ACCCTGAACC CTGAGGACAG AAGAGAATAC 
CCTGATCTCC AGGCTGCCCA CCGGCCATTC CCTAAGCAAC GATTCAGGCA GGAAAATGGG 
CATACATCCT TACAAAGAGA TGGACCTAGA TCTTTCCTCC TGGATCTCCC AAACTTTCCT 
GACCTGTCAA AAGCAGATCT CAACGGGCAG AATCCCAACA TTCAGGTTAC CATTGAAGTG 
GTGGATGGCC CCGACACAGA GACCGACAAG GACCTTCAGA AAGAGAGCAG CAAGTCTAGC 
TGGCCAGTCT CATCACCTGA CTGGAGAAAC TGGTGGCAGA GGTCCTCCAC CTTGACTCGC 
ATGAGCAGCG GTGACCAGGA CTACAAGTAT GACAGCACTA CTGAGGACAG CAACTTCCTG 
AACCCTCTTG GTGGGTGGGA TGGTCATGCA CCCAGTCACC GGACATTTGA GTCCAAGGAG 
CAGCCAGAGT ATGACTACAT TGATGGTGAG GGAGACTGGA GCCCATGGTC CCTTTGTAGC 
GTCACCTGTG GAAGCGGCAG TCAGAAGCGG ACGAGGTCCT GTGGGTATGC CTGTACTGCC 
ACCGAGTCAC GGACCTGCGA CAGGCCAAAC TGCCCAGGGA TTGAAGATAC CTTCCGGACT 
GCAGCCACAG AAGTGAGCTT ACTCGCTGGG AGCGAAGACT TCAATGCTAC CAAACTGTTT 
GAAGTTGATA CTGATAGCTG TGAAAGATGG ATGAGCTGCA AAAGTGAGTT CTTGAAGAAA 
TACATGCACA AAGTGGTCAA CGATCTTCCC AGCTGTCCTT GCTCCTACCC CAGAGAAGTT 
GCTTACAGCA CTGCTGACAT CTACGATCGG ATTAAGAGGA AAAACTTTCG TTGGAAAGAT 
GCAAGTGGTC CAAAAGAAAA ACTGGAGATC TATAAGCCTA CTGCACGGTA CTGCATCCGC 
TCCATGCTCT CTTTGGAGAG CACAACCCTG GCAGCTCAAC ACTGCTGCTA TGATGATAAC 
ATGCAGCTGA TTACCAGAGG GAAAGGGGCA GGTACACCAA ACCTGATCAG CATCGAATTC 
TCAGCAGAAC TCCATTACAA AGTGGACATT CTGCCCTGGA TTATATGCAA AGGTGACTGG 
AGCCGGTACA ATGAAGCACG GCCTCCAAAC AATGGGCAGA AGTGTACAGA AAACCCTACA 
GATGAAGACT ATTACAAGCA ATTTCAAGAA GCAAGGGAAT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015139011.1
CDS	62..1786
Translation	MEPGLLAETPPARGPAATCRPPRGSHGIPAAASAASRPDVAPPWCGGPRCCCCCCCRRCPADAESGAVPAADPGAAPAAAAAPAAAAAAPPPPPPPPRPPPPRGPKAARLKRMVRLAAELLLLLGLLLLTLHITVLRGGGGEPQGPAGAANHSQRQNTLTADDHSPEDSFTLNPEDRREYPDLQAAHRPFPKQRFRQENGHTSLQRDGPRSFLLDLPNFPDLSKADLNGQNPNIQVTIEVVDGPDTETDKDLQKESSKSSWPVSSPDWRNWWQRSSTLTRMSSGDQDYKYDSTTEDSNFLNPLGGWDGHAPSHRTFESKEQPEYDYIDGEGDWSPWSLCSVTCGSGSQKRTRSCGYACTATESRTCDRPNCPGIEDTFRTAATEVSLLAGSEDFNATKLFEVDTDSCERWMSCKSEFLKKYMHKVVNDLPSCPCSYPREVAYSTADIYDRIKRKNFRWKDASGPKEKLEIYKPTARYCIRSMLSLESTTLAAQHCCYDDNMQLITRGKGAGTPNLISIEFSAELHYKVDILPWIICKGDWSRYNEARPPNNGQKCTENPTDEDYYKQFQEAREY

Target ORF information:

RefSeq Version	XM_015283525.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus isthmin 1, angiogenesis inhibitor (ISM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015283525.1

ORF Insert Sequence:

ATGGAGCCAG GGCTGCTCGC GGAGACGCCG CCTGCCCGCG GCCCCGCCGC TACCTGCCGC 
CCGCCGCGGG GCAGCCACGG GATTCCCGCC GCCGCCTCCG CCGCGTCCCG CCCCGACGTG 
GCTCCGCCGT GGTGTGGCGG CCCCCGCTGC TGCTGCTGCT GCTGCTGCCG CCGCTGCCCC 
GCCGACGCCG AGAGCGGCGC GGTCCCGGCG GCCGACCCCG GGGCTGCTCC GGCGGCGGCG 
GCGGCTCCGG CGGCGGCGGC CGCGGCTCCT CCTCCTCCTC CGCCTCCTCC TCGGCCGCCG 
CCGCCGCGGG GCCCTAAAGC CGCCCGGCTC AAGAGGATGG TGCGGCTGGC GGCGGAGCTG 
CTGCTGCTGC TGGGGCTGCT CCTGCTCACC TTGCACATCA CCGTGCTGCG CGGCGGCGGC 
GGAGAGCCGC AGGGCCCCGC GGGCGCCGCC AACCACAGCC AGCGGCAGAA CACGCTCACT 
GCAGATGACC ATTCTCCTGA AGACAGCTTT ACCCTGAACC CTGAGGACAG AAGAGAATAC 
CCTGATCTCC AGGCTGCCCA CCGGCCATTC CCTAAGCAAC GATTCAGGCA GGAAAATGGG 
CATACATCCT TACAAAGAGA TGGACCTAGA TCTTTCCTCC TGGATCTCCC AAACTTTCCT 
GACCTGTCAA AAGCAGATCT CAACGGGCAG AATCCCAACA TTCAGGTTAC CATTGAAGTG 
GTGGATGGCC CCGACACAGA GACCGACAAG GACCTTCAGA AAGAGAGCAG CAAGTCTAGC 
TGGCCAGTCT CATCACCTGA CTGGAGAAAC TGGTGGCAGA GGTCCTCCAC CTTGACTCGC 
ATGAGCAGCG GTGACCAGGA CTACAAGTAT GACAGCACTA CTGAGGACAG CAACTTCCTG 
AACCCTCTTG GTGGGTGGGA TGGTCATGCA CCCAGTCACC GGACATTTGA GTCCAAGGAG 
CAGCCAGAGT ATGACTACAT TGATGGTGAG GGAGACTGGA GCCCATGGTC CCTTTGTAGC 
GTCACCTGTG GAAGCGGCAG TCAGAAGCGG ACGAGGTCCT GTGGGTATGC CTGTACTGCC 
ACCGAGTCAC GGACCTGCGA CAGGCCAAAC TGCCCAGGGA TTGAAGATAC CTTCCGGACT 
GCAGCCACAG AAGTGAGCTT ACTCGCTGGG AGCGAAGACT TCAATGCTAC CAAACTGTTT 
GAAGTTGATA CTGATAGCTG TGAAAGATGG ATGAGCTGCA AAAGTGAGTT CTTGAAGAAA 
TACATGCACA AAGTGGTCAA CGATCTTCCC AGCTGTCCTT GCTCCTACCC CAGAGAAGTT 
GCTTACAGCA CTGCTGACAT CTACGATCGG ATTAAGAGGA AAAACTTTCG TTGGAAAGAT 
GCAAGTGGTC CAAAAGAAAA ACTGGAGATC TATAAGCCTA CTGCACGGTA CTGCATCCGC 
TCCATGCTCT CTTTGGAGAG CACAACCCTG GCAGCTCAAC ACTGCTGCTA TGATGATAAC 
ATGCAGCTGA TTACCAGAGG GAAAGGGGCA GGTACACCAA ACCTGATCAG CATCGAATTC 
TCAGCAGAAC TCCATTACAA AGTGGACATT CTGCCCTGGA TTATATGCAA AGGTGACTGG 
AGCCGGTACA ATGAAGCACG GCCTCCAAAC AATGGGCAGA AGTGTACAGA AAACCCTACA 
GATGAAGACT ATTACAAGCA ATTTCAAGAA GCAAGGGAAT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ISM1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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