EFL1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	EFL1
Entrez Gene ID	415467
Full Name	elongation factor like GTPase 1
Synonyms	EFTUD1
General protein information	Preferred Names elongation factor like GTPase 1 Names elongation factor-like GTPase 1 elongation factor Tu GTP binding domain containing 1 elongation factor Tu GTP-binding domain-containing protein 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001039256.2	NP_001034345.2	elongation factor-like GTPase 1
XM_025153836.1	XP_025009604.1	elongation factor-like GTPase 1 isoform X1

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os03g0650700	NP_001050786.1
Magnaporthe oryzae (rice blast fungus)	MGG_05265	XP_003712823.1
Schizosaccharomyces pombe (fission yeast)	SPCC553.08c	NP_587766.1
Drosophila melanogaster (fruit fly)	CG33158	NP_788515.1
Arabidopsis thaliana (thale cress)	AT3G22980	NP_188938.1
Eremothecium gossypii	AGOS_AFR031C	NP_985578.2
Bos taurus (cattle)	EFTUD1	XP_002696652.1
Danio rerio (zebrafish)	eftud1	XP_697184.5
Saccharomyces cerevisiae (baker's yeast)	RIA1	NP_014236.1
Pan troglodytes (chimpanzee)	EFTUD1	XP_510546.2
Macaca mulatta (Rhesus monkey)	EFTUD1	XP_002804960.1
Mus musculus (house mouse)	Eftud1	NP_780526.2
Rattus norvegicus (Norway rat)	Eftud1	NP_001101004.1
Gallus gallus (chicken)	EFTUD1	NP_001034345.2
Kluyveromyces lactis	KLLA0D07898g	XP_453415.1
Oryza sativa (rice)	Os02g0543300	NP_001047074.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003001	XP_311881.3
Homo sapiens (human)	EFTUD1	NP_078856.4
Neurospora crassa	NCU06278	XP_962882.2
Canis lupus familiaris (dog)	EFTUD1	XP_850111.1
Xenopus tropicalis (tropical clawed frog)	eftud1	XP_002940146.2

Gene Ontology
Bibliography

Related articles in PubMed

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Caldwell RB, Kierzek AM, Arakawa H, Bezzubov Y, Zaim J, Fiedler P, Kutter S, Blagodatski A, Kostovska D, Koter M, Plachy J, Carninci P, Hayashizaki Y, Buerstedde JM
Genome biology6(1)R6(2005)

A comprehensive collection of chicken cDNAs.
Boardman PE, Sanz-Ezquerro J, Overton IM, Burt DW, Bosch E, Fong WT, Tickle C, Brown WR, Wilson SA, Hubbard SJ
Current biology : CB12(22)1965-9(2002 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EFL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EFL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa143387	XM_025153836.1 Latest version!	Gallus gallus elongation factor like GTPase 1 (EFL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OGa24087	NM_001039256.2 Latest version!	Gallus gallus elongation factor like GTPase 1 (EFL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EFL1 ( XM_025153836.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025009604.1 Gallus gallus elongation factor like GTPase 1 (EFL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa143387
Clone ID Related Accession (Same CDS sequence)	XM_025153836.1
Accession Version	XM_025153836.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	elongation factor-like GTPase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCTTA AGAATATTTT AGAACAGATA AATGCAGTCA CTGGATCACT CTTTACCTCG 
AAAGTCTTAG AAGAAAGAGC TGAAAAAGAA GCCGAAAGTG AAAGTAGTTC TGACACAGCA 
GCTGGAGATC AAATTTATGA CTGGAGTGCT GGGTTGGAAG ATACTGATGA TTCCCACCTC 
TATTTTTCTC CAGACCATGG AAATGTGGTG TTTGCCAGTG CAATTGATGG CTGGGGTTTT 
GGCATTGAGC ATTTTGCAAA ACTGTACAGC CAAAAGATTG GAATCAAACC TGCAGTGCTG 
CTGAAGACTT TATGGGGAGA TTATTATCTA AACACAAAAG CAAAGAAGAT AATGAAAGGT 
GATCAGTCAA AAGGAAAGAA GCCCTTGTTT GTGCAGTTGG TGTTGGACAA CATATGGAGT 
CTATATGAAG CTGTTATGAA AAGAGATAAA GAAAAAATTG AGAAGATAGT TACCTCATTG 
GGCTTGAAAA TTGGTCCTCG GGAGTCACGA CACGCAGACC CCAAAGTTCA CCTCAATGCC 
ATTTGTAGTC AGTGGCTGCC TATATCTGAT GCAGTCCTTT CTATGGTGTG TAGTAAACTT 
CCCAGTCCCC TTGACATCAC TGCAGAGAGA GTGGAAAAGC TGATGTGTGT TGGAGCTAAA 
ACATTTGATT CTTTACCACC GGAAACCCAG GAACTGAAAA GCGCTTTTCT GAGATGCAGT 
AGTGAAGAAA CGGCCCCGGT GATTGTTTTT GTTTCCAAAA TGTTTGCTGT TGACGCAAAG 
GCATTGCCAC ACAATAAACC AAGGCCTTTA ACCCAAGAAG AAATAGCTCA GCGACGTGAA 
CTTGCAAAAC AGAGGCATGC AGGAAAAATG GCAGCAAATC AAGGGAAGGA GGTTTCTGAA 
AAGCCCCCAA GTGAAAATTC ATCTGAAGGG ACTGGAAGCA CTGGTGATAA GAAAGGTGAA 
GAGCAACAGC TCACTGATCA GATGGACGTC GTGACCTCAA AGGCAGCGAA ACAGGAAGCA 
GACAACAAGG AGTCTTTCAT TGCTTTTGCT AGAGTGTTCA GCGGTGTGGT ACGAAGAGGG 
CAAAAGATCT TTGTTTTGGG ACCCAAATAT GATCCTGCTG AGTCTCTGCA TAAGCTGTCA 
CCCCAGTGTT CTGCTACAGA TGATCTGCCA TCAGTACCTC ATATGTCTTG CTGTACATTG 
GAGAATCTAT ACTTGCTCAT GGGAAGAGAA CTTGAAGAGC TTGAAGAAGT ACCTGCAGGA 
AATGTTCTAG GAATAGGAGG CCTGCAAGAT TTTGTTCTAA AGTCAGCAAC TTTGTCAACG 
TCACCAGCTT GCCCACCTTT TACACCTCTC AACTTTGAGG CTACACCAAT TGTACGGGTA 
GCTGTTGAAC CAAAACATCC AAGTGATATG CCTCAGCTGG TGAGAGGAAT GAAGCTTTTA 
AACCAAGCTG ATCCGTGTGT CCAAGTTTTG ATCCAGGAGA CAGGAGAGCA TGTACTTGTG 
ACAGCAGGAG AAGTTCATCT GCAGCGTTGC TTGGATGACC TGAAGGAAAG GTTTGCAAAG 
ATAGAGATTA GTGTGTCAGC ACCTATTATT CCATTTCGAG AAACAATAAC GAGACCTCCA 
AAAGTCGACA TGGTAAATGA AGAAATAGGA AAACAGCAGA AAGTTGCTGT CATACACCAA 
ACAAAAGAGG ACCAAAATAA AATTCCTGAA GGAATTCAGG TCGATTCAGA TGGGCTTGTT 
ACAATATCTA CTCCAAATAA ACAAGCTACT CTCAGTGTAA GAGCATTACC ACTTCCTGAG 
GAGGTAACTC GACTTCTTGA AGAAAACAGT GACTTAATTC GAACTATGGA GCAACTCAGC 
ACGTCTCTGA ATGAAGATAA AAATGCTCAT GAAATAAATC AGAAGACTAT GGACAAAATA 
AAGGAATTCA AACAAAAATT AGAGCAAAAT CTGCAGGGAA GGAAGTGGAG AAATGCAGTT 
GATCATATCT GGTCATTTGG ACCACGGAAA TGTGGGCCTA ATATTTTGTT GCATAAATTT 
GAGGGTTATA AAAATTCTGT GTGGCAGTGC CTTGAGAATA CTGAGAAAGA AGTAAGTAAG 
TATGGAGATT TTGACAACAG CATAGTAAGT GGATTTCAGC TGGCTACGCT TTCAGGTCCC 
ATGTGTGAAG AACCCCTCAT GGGTGTGTGT TTTACAGTGG AAAAATGGGA AATGAATAAG 
GTTGGAGAGA CGCTTCTAAC TAAAAGTCGG GATTCAGGGG AATGTGGTGC TGTAGAACAT 
CAAGAAAGTG AAGATACTGT TTCTCCAACA AGCAATTGCA CTGTTGACAC TTGCAGTGAG 
AATGAACACG AACAGAGCAG TCAAAGTGTT CCAAAGTCAC TTGAAAAAAA TAGTAAAAAC 
AAGGGAGAAG TTTTACTTAC AGATTGTTAT GGTCCCTTCT CTGGACAGCT GATAGCCACC 
ATGAAGGAAG CATGTCGTTA TGCTTTGCAA GCAAAACCAC AGAGGCTTAT GGCAGCAATG 
TATACCTGCG AAATTATGGC TACTGCGGAA GTTCTAGGTC GTGTGTATGC CGTCTTGTCC 
AAAAGAGAAG GCAGAGTGTT ACAAGAGGAG ATGAAAGAAG GAACGGATGT GTTTATTATT 
AAGGCAGTCC TGCCAGTAGC AGAAAGCTTT GGTTTTGCTG ATGAAATCAG GAAGAGAACT 
AGTGGCCTGG CCAGTCCGCA GCTGGTGTTC AGCCACTGGG AGATCATTTC AAGTGATCCA 
TTCTGGGTAC CAACCACCGA AGAAGAATAT TTGCACTTTG GGGAAAAGGC AGACTCTGAG 
AACCAGGCCC GCAAGTACAT GAATGCAGTG AGAAAGCGGA AGGGACTTTA TGTAGAAGAA 
AAAATTGTGG AGCATGCTGA AAAGCAAAGA ACCCTCAGTA GGAATAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025009604.1
CDS	418..3348
Translation	MHLKNILEQINAVTGSLFTSKVLEERAEKEAESESSSDTAAGDQIYDWSAGLEDTDDSHLYFSPDHGNVVFASAIDGWGFGIEHFAKLYSQKIGIKPAVLLKTLWGDYYLNTKAKKIMKGDQSKGKKPLFVQLVLDNIWSLYEAVMKRDKEKIEKIVTSLGLKIGPRESRHADPKVHLNAICSQWLPISDAVLSMVCSKLPSPLDITAERVEKLMCVGAKTFDSLPPETQELKSAFLRCSSEETAPVIVFVSKMFAVDAKALPHNKPRPLTQEEIAQRRELAKQRHAGKMAANQGKEVSEKPPSENSSEGTGSTGDKKGEEQQLTDQMDVVTSKAAKQEADNKESFIAFARVFSGVVRRGQKIFVLGPKYDPAESLHKLSPQCSATDDLPSVPHMSCCTLENLYLLMGRELEELEEVPAGNVLGIGGLQDFVLKSATLSTSPACPPFTPLNFEATPIVRVAVEPKHPSDMPQLVRGMKLLNQADPCVQVLIQETGEHVLVTAGEVHLQRCLDDLKERFAKIEISVSAPIIPFRETITRPPKVDMVNEEIGKQQKVAVIHQTKEDQNKIPEGIQVDSDGLVTISTPNKQATLSVRALPLPEEVTRLLEENSDLIRTMEQLSTSLNEDKNAHEINQKTMDKIKEFKQKLEQNLQGRKWRNAVDHIWSFGPRKCGPNILLHKFEGYKNSVWQCLENTEKEVSKYGDFDNSIVSGFQLATLSGPMCEEPLMGVCFTVEKWEMNKVGETLLTKSRDSGECGAVEHQESEDTVSPTSNCTVDTCSENEHEQSSQSVPKSLEKNSKNKGEVLLTDCYGPFSGQLIATMKEACRYALQAKPQRLMAAMYTCEIMATAEVLGRVYAVLSKREGRVLQEEMKEGTDVFIIKAVLPVAESFGFADEIRKRTSGLASPQLVFSHWEIISSDPFWVPTTEEEYLHFGEKADSENQARKYMNAVRKRKGLYVEEKIVEHAEKQRTLSRNK

Target ORF information:

RefSeq Version	XM_025153836.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus elongation factor like GTPase 1 (EFL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025153836.1

ORF Insert Sequence:

ATGCATCTTA AGAATATTTT AGAACAGATA AATGCAGTCA CTGGATCACT CTTTACCTCG 
AAAGTCTTAG AAGAAAGAGC TGAAAAAGAA GCCGAAAGTG AAAGTAGTTC TGACACAGCA 
GCTGGAGATC AAATTTATGA CTGGAGTGCT GGGTTGGAAG ATACTGATGA TTCCCACCTC 
TATTTTTCTC CAGACCATGG AAATGTGGTG TTTGCCAGTG CAATTGATGG CTGGGGTTTT 
GGCATTGAGC ATTTTGCAAA ACTGTACAGC CAAAAGATTG GAATCAAACC TGCAGTGCTG 
CTGAAGACTT TATGGGGAGA TTATTATCTA AACACAAAAG CAAAGAAGAT AATGAAAGGT 
GATCAGTCAA AAGGAAAGAA GCCCTTGTTT GTGCAGTTGG TGTTGGACAA CATATGGAGT 
CTATATGAAG CTGTTATGAA AAGAGATAAA GAAAAAATTG AGAAGATAGT TACCTCATTG 
GGCTTGAAAA TTGGTCCTCG GGAGTCACGA CACGCAGACC CCAAAGTTCA CCTCAATGCC 
ATTTGTAGTC AGTGGCTGCC TATATCTGAT GCAGTCCTTT CTATGGTGTG TAGTAAACTT 
CCCAGTCCCC TTGACATCAC TGCAGAGAGA GTGGAAAAGC TGATGTGTGT TGGAGCTAAA 
ACATTTGATT CTTTACCACC GGAAACCCAG GAACTGAAAA GCGCTTTTCT GAGATGCAGT 
AGTGAAGAAA CGGCCCCGGT GATTGTTTTT GTTTCCAAAA TGTTTGCTGT TGACGCAAAG 
GCATTGCCAC ACAATAAACC AAGGCCTTTA ACCCAAGAAG AAATAGCTCA GCGACGTGAA 
CTTGCAAAAC AGAGGCATGC AGGAAAAATG GCAGCAAATC AAGGGAAGGA GGTTTCTGAA 
AAGCCCCCAA GTGAAAATTC ATCTGAAGGG ACTGGAAGCA CTGGTGATAA GAAAGGTGAA 
GAGCAACAGC TCACTGATCA GATGGACGTC GTGACCTCAA AGGCAGCGAA ACAGGAAGCA 
GACAACAAGG AGTCTTTCAT TGCTTTTGCT AGAGTGTTCA GCGGTGTGGT ACGAAGAGGG 
CAAAAGATCT TTGTTTTGGG ACCCAAATAT GATCCTGCTG AGTCTCTGCA TAAGCTGTCA 
CCCCAGTGTT CTGCTACAGA TGATCTGCCA TCAGTACCTC ATATGTCTTG CTGTACATTG 
GAGAATCTAT ACTTGCTCAT GGGAAGAGAA CTTGAAGAGC TTGAAGAAGT ACCTGCAGGA 
AATGTTCTAG GAATAGGAGG CCTGCAAGAT TTTGTTCTAA AGTCAGCAAC TTTGTCAACG 
TCACCAGCTT GCCCACCTTT TACACCTCTC AACTTTGAGG CTACACCAAT TGTACGGGTA 
GCTGTTGAAC CAAAACATCC AAGTGATATG CCTCAGCTGG TGAGAGGAAT GAAGCTTTTA 
AACCAAGCTG ATCCGTGTGT CCAAGTTTTG ATCCAGGAGA CAGGAGAGCA TGTACTTGTG 
ACAGCAGGAG AAGTTCATCT GCAGCGTTGC TTGGATGACC TGAAGGAAAG GTTTGCAAAG 
ATAGAGATTA GTGTGTCAGC ACCTATTATT CCATTTCGAG AAACAATAAC GAGACCTCCA 
AAAGTCGACA TGGTAAATGA AGAAATAGGA AAACAGCAGA AAGTTGCTGT CATACACCAA 
ACAAAAGAGG ACCAAAATAA AATTCCTGAA GGAATTCAGG TCGATTCAGA TGGGCTTGTT 
ACAATATCTA CTCCAAATAA ACAAGCTACT CTCAGTGTAA GAGCATTACC ACTTCCTGAG 
GAGGTAACTC GACTTCTTGA AGAAAACAGT GACTTAATTC GAACTATGGA GCAACTCAGC 
ACGTCTCTGA ATGAAGATAA AAATGCTCAT GAAATAAATC AGAAGACTAT GGACAAAATA 
AAGGAATTCA AACAAAAATT AGAGCAAAAT CTGCAGGGAA GGAAGTGGAG AAATGCAGTT 
GATCATATCT GGTCATTTGG ACCACGGAAA TGTGGGCCTA ATATTTTGTT GCATAAATTT 
GAGGGTTATA AAAATTCTGT GTGGCAGTGC CTTGAGAATA CTGAGAAAGA AGTAAGTAAG 
TATGGAGATT TTGACAACAG CATAGTAAGT GGATTTCAGC TGGCTACGCT TTCAGGTCCC 
ATGTGTGAAG AACCCCTCAT GGGTGTGTGT TTTACAGTGG AAAAATGGGA AATGAATAAG 
GTTGGAGAGA CGCTTCTAAC TAAAAGTCGG GATTCAGGGG AATGTGGTGC TGTAGAACAT 
CAAGAAAGTG AAGATACTGT TTCTCCAACA AGCAATTGCA CTGTTGACAC TTGCAGTGAG 
AATGAACACG AACAGAGCAG TCAAAGTGTT CCAAAGTCAC TTGAAAAAAA TAGTAAAAAC 
AAGGGAGAAG TTTTACTTAC AGATTGTTAT GGTCCCTTCT CTGGACAGCT GATAGCCACC 
ATGAAGGAAG CATGTCGTTA TGCTTTGCAA GCAAAACCAC AGAGGCTTAT GGCAGCAATG 
TATACCTGCG AAATTATGGC TACTGCGGAA GTTCTAGGTC GTGTGTATGC CGTCTTGTCC 
AAAAGAGAAG GCAGAGTGTT ACAAGAGGAG ATGAAAGAAG GAACGGATGT GTTTATTATT 
AAGGCAGTCC TGCCAGTAGC AGAAAGCTTT GGTTTTGCTG ATGAAATCAG GAAGAGAACT 
AGTGGCCTGG CCAGTCCGCA GCTGGTGTTC AGCCACTGGG AGATCATTTC AAGTGATCCA 
TTCTGGGTAC CAACCACCGA AGAAGAATAT TTGCACTTTG GGGAAAAGGC AGACTCTGAG 
AACCAGGCCC GCAAGTACAT GAATGCAGTG AGAAAGCGGA AGGGACTTTA TGTAGAAGAA 
AAAATTGTGG AGCATGCTGA AAAGCAAAGA ACCCTCAGTA GGAATAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFL1 ( NM_001039256.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001034345.2 Gallus gallus elongation factor like GTPase 1 (EFL1), mRNA.

Price & Availability↑

CloneID	OGa24087
Clone ID Related Accession (Same CDS sequence)	NM_001039256.2
Accession Version	NM_001039256.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526745600000
Organism	Gallus gallus(chicken)
Product	elongation factor-like GTPase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AJ851470.1, BU240511.1 and BX259610.2. On Apr 9, 2013 this sequence version replaced NM_001039256.1. Sequence Note: This RefSeq record was created from transcript data from different strains because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AJ851470.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTCCTTA CGAGCGTGGG GAAGATGATG GGCCTCCAGC AAAAGGCTGC TGGCATCAGG 
AACATCTGTG TGCTGGCGCA CGTGGATCAC GGCAAAACCA CGCTAGCAGA TTGCTTGATA 
TCCAGCAATG GGATAATCTC CAGCCGCTTA GCAGGAAAGC TGCGGTATCT GGACAGCAGG 
GAGGATGAGC AGGTCCGAGG AATAACCATG AAATCTAGTG CCATTTCGTT GCACTTTGAG 
AAAGGTGGTC AGGAGTATCT GATTAATTTA ATAGACTCCC CAGGGCATGT GGATTTTTCT 
TCAGAAGTAT CTACTGCTGT CCGACTCTGT GATGGTTGCA TCATAGTGGT GGATGCTGTT 
GAAGGCGTCT GTCCACAGAC TCAAGCAGTT CTACGGCAGG CGTGGCTAGA AAATATTCGT 
CCAGTATTGG TGATAAATAA AATTGATCGC TTGATTGTGG AGCTGAAGTT AACCCCTCAG 
GAGGCATACA TGCACCTTAA GAATATTTTA GAACAGATAA ATGCAGTCAC TGGATCACTC 
TTTACCTCGA AAGTCTTAGA AGAAAGAGCT GAAAAAGAAG CCGAAAGTGA AAGTAGTTCT 
GACACAGCAG CTGGAGATCA AATTTATGAC TGGAGTGCTG GGTTGGAAGA TACTGATGAT 
TCCCACCTCT ATTTTTCTCC AGACCATGGA AATGTGGTGT TTGCCAGTGC AATTGATGGC 
TGGGGTTTTG GCATTGAGCA TTTTGCAAAA CTGTACAGCC AAAAGATTGG AATCAAACCT 
GCAGTGCTGC TGAAGACTTT ATGGGGAGAT TATTATCTAA ACACAAAAGC AAAGAAGATA 
ATGAAAGGTG ATCAGTCAAA AGGAAAGAAG CCCTTGTTTG TGCAGTTGGT GTTGGACAAC 
ATATGGAGTC TATATGAAGC TGTTATGAAA AGAGATAAAG AAAAAATTGA GAAGATAGTT 
ACCTCATTGG GCTTGAAAAT TGGTCCTCGG GAGTCACGGC ACGCAGACCC CAAAGTTCAC 
CTCAATGCCA TTTGTAGTCA GTGGCTGCCT ATATCTGATG CAGTCCTTTC TATGGTGTGT 
AGTAAACTTC CCAGTCCCCT TGACATCACT GCAGAGAGAG TGGAAAAGCT GATGTGTGTT 
GGAGCTAAAA CATTTGATTC TTTACCACCG GAAACCCAGG AACTGAAAAG CGCTTTTCTG 
AGATGCAGTA GTGAAGAAAC GGCCCCAGTG ATTGTTTTTG TTTCCAAAAT GTTTGCTGTT 
GACGCAAAGG CATTGCCACA CAATAAACCA AGGCCTTTAA CCCAAGAAGA AATAGCTCAG 
CGACGTGAAC TTGCAAAACA GAGGCATGCA GGAAAAATGG CAGCAAGTCA AGGGAAGGAG 
GTTTCTGAAA AGCCCCCAAG TGAAAATTCA TCTGAAGGGA CTGGAAGCAC TGGTGATAAG 
AAAGGTGAAG AGCAACAGCT CACTGATCAG ATGGACGTCG TGACCTCAAA GGCAGCCAAA 
CAGGAAGCAG ACAACAAGGA GTCTTTCATT GCTTTTGCTA GAGTGTTCAG CGGTGTGGTA 
CGAAGAGGGC AAAAGATCTT TGTTTTGGGA CCCAAATATG ATCCTGCTGA GTCTCTGCAT 
AAGCTGTCAC CCCAGTGTTC TGCTACAGAT GATCTGCCAT CAGTACCTCA TATGTCTTGC 
TGTACATTGG AGAATCTATA CTTGCTCATG GGAAGAGAAC TTGAAGAGCT TGAAGAAGTA 
CCTGCAGGAA ATGTTCTAGG AATAGGAGGC CTGCAAGATT TTGTTCTAAA GTCAGCAACT 
TTGTCAACGT CACCAGCTTG CCCACCTTTT ACACCTCTCA ACTTTGAGGC TACACCAATT 
GTACGGGTAG CTGTTGAACC AAAACATCCA AGTGATATGC CTCAGCTGGT GAGAGGAATG 
AAGCTTTTAA ACCAAGCTGA TCCGTGTGTC CAAGTTTTGA TCCAGGAGAC AGGAGAGCAT 
GTACTTGTGA CAGCAGGAGA AGTTCATCTG CAGCGTTGCT TGGATGACCT GAAGGAAAGG 
TTTGCAAAGA TAGAGATTAG TGTGTCAGCA CCTATAATTC CATTTCGAGA AACAATAACG 
AGACCTCCAA AAGTCGACAT GGTAAATGAA GAAATAGGAA AACAGCAGAA AGTTGCTGTC 
ATACACCAAA CAAAAGAGGA CCAAAATAAA ATTCCTGAAG GAATTCAGGT CGATTCAGAT 
GGGCTTGTTA CAATATCTAC TCCAAATAAA CAAGCTACTC TCAGTGTAAG AGCATTACCA 
CTTCCTGAGG AGGTAACTCG ACTTCTTGAA GAAAACAGTG ACTTAATTCG AACTATGGAG 
CAACTCAGCA CGTCTCTGAA TGAAGATAAA AATGCTCATG AAATAAATCA GAAGACTATG 
GACAAAATAA AGGAATTCAA ACAAAAATTA GAGCAAAATC TGCAGGGAAG GAAGTGGAGA 
AATGCAGTTG ATCATATCTG GTCATTTGGA CCACGGAAAT GTGGGCCTAA TATTTTGTTG 
CATAAATTTG AGGGTTATAA AAATTCTGTG TGGCAGTGCC TTGAGAATAC TGAGAAAGAA 
GTAAGTAAGT ATGGAGATTT TGACAACAGC ATAGTAAGTG GATTTCAGCT GGCTACGCTT 
TCAGGTCCCA TGTGTGAAGA ACCCCTCATG GGTGTGTGTT TTACAGTGGA AAAATGGGAA 
ATGAATAAGG TTGGAGAGAC GCTTCTAACT AAAAGTCGGG ATTCAGGGGA ATGTGGTGCT 
GTAGAACATC AAGAAAGTGA AGATACTGTT TCTCCAACAA GCAATTGCAC TGTTGACACT 
TGCAGTGAGA ATGAACACGA ACAGAGCAGT CAAAGTGTTC CAAAGTCACT TGAAAAAAAT 
AGTAAAAACA AGGGAGAAGT TTTACTTACA GATTGTTATG GTCCCTTCTC TGGACAGCTG 
ATTGCCACCA TGAAGGAAGC ATGTCGTTAT GCTTTGCAAG CAAAACCACA GAGGCTTATG 
GCAGCAATGT ATACCTGCGA AATTATGGCT ACTGCGGAAG TTCTAGGTCG TGTGTATGCC 
GTCTTGTCCA AAAGAGAAGG CAGAGTGTTA CAAGAGGAGA TGAAAGAAGG AACGGATGTG 
TTCATTATTA AGGCAGTCCT GCCAGTAGCA GAAAGCTTTG GTTTTGCTGA TGAAATCAGG 
AAGAGAACTA GTGGCCTGGC CAGTCCGCAG CTGGTGTTCA GCCACTGGGA GATCATTTCA 
AGTGATCCAT TCTGGGTACC AACCACCGAA GAAGAATATT TGCACTTTGG GGAAAAGGCA 
GACTCTGAGA ACCAGGCCCG CAAGTACATG AATGCAGTGA GAAAGCGGAA GGGACTTTAT 
GTAGAAGAAA AAATTGTGGA GCATGCTGAA AAGCAAAGAA CCCTCAGTAG GAATAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034345.2
CDS	66..3485
Translation	MVLTSVGKMMGLQQKAAGIRNICVLAHVDHGKTTLADCLISSNGIISSRLAGKLRYLDSREDEQVRGITMKSSAISLHFEKGGQEYLINLIDSPGHVDFSSEVSTAVRLCDGCIIVVDAVEGVCPQTQAVLRQAWLENIRPVLVINKIDRLIVELKLTPQEAYMHLKNILEQINAVTGSLFTSKVLEERAEKEAESESSSDTAAGDQIYDWSAGLEDTDDSHLYFSPDHGNVVFASAIDGWGFGIEHFAKLYSQKIGIKPAVLLKTLWGDYYLNTKAKKIMKGDQSKGKKPLFVQLVLDNIWSLYEAVMKRDKEKIEKIVTSLGLKIGPRESRHADPKVHLNAICSQWLPISDAVLSMVCSKLPSPLDITAERVEKLMCVGAKTFDSLPPETQELKSAFLRCSSEETAPVIVFVSKMFAVDAKALPHNKPRPLTQEEIAQRRELAKQRHAGKMAASQGKEVSEKPPSENSSEGTGSTGDKKGEEQQLTDQMDVVTSKAAKQEADNKESFIAFARVFSGVVRRGQKIFVLGPKYDPAESLHKLSPQCSATDDLPSVPHMSCCTLENLYLLMGRELEELEEVPAGNVLGIGGLQDFVLKSATLSTSPACPPFTPLNFEATPIVRVAVEPKHPSDMPQLVRGMKLLNQADPCVQVLIQETGEHVLVTAGEVHLQRCLDDLKERFAKIEISVSAPIIPFRETITRPPKVDMVNEEIGKQQKVAVIHQTKEDQNKIPEGIQVDSDGLVTISTPNKQATLSVRALPLPEEVTRLLEENSDLIRTMEQLSTSLNEDKNAHEINQKTMDKIKEFKQKLEQNLQGRKWRNAVDHIWSFGPRKCGPNILLHKFEGYKNSVWQCLENTEKEVSKYGDFDNSIVSGFQLATLSGPMCEEPLMGVCFTVEKWEMNKVGETLLTKSRDSGECGAVEHQESEDTVSPTSNCTVDTCSENEHEQSSQSVPKSLEKNSKNKGEVLLTDCYGPFSGQLIATMKEACRYALQAKPQRLMAAMYTCEIMATAEVLGRVYAVLSKREGRVLQEEMKEGTDVFIIKAVLPVAESFGFADEIRKRTSGLASPQLVFSHWEIISSDPFWVPTTEEEYLHFGEKADSENQARKYMNAVRKRKGLYVEEKIVEHAEKQRTLSRNK

Target ORF information:

RefSeq Version	NM_001039256.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus elongation factor like GTPase 1 (EFL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039256.2

ORF Insert Sequence:

ATGGTCCTTA CGAGCGTGGG GAAGATGATG GGCCTCCAGC AAAAGGCTGC TGGCATCAGG 
AACATCTGTG TGCTGGCGCA CGTGGATCAC GGCAAAACCA CGCTAGCAGA TTGCTTGATA 
TCCAGCAATG GGATAATCTC CAGCCGCTTA GCAGGAAAGC TGCGGTATCT GGACAGCAGG 
GAGGATGAGC AGGTCCGAGG AATAACCATG AAATCTAGTG CCATTTCGTT GCACTTTGAG 
AAAGGTGGTC AGGAGTATCT GATTAATTTA ATAGACTCCC CAGGGCATGT GGATTTTTCT 
TCAGAAGTAT CTACTGCTGT CCGACTCTGT GATGGTTGCA TCATAGTGGT GGATGCTGTT 
GAAGGCGTCT GTCCACAGAC TCAAGCAGTT CTACGGCAGG CGTGGCTAGA AAATATTCGT 
CCAGTATTGG TGATAAATAA AATTGATCGC TTGATTGTGG AGCTGAAGTT AACCCCTCAG 
GAGGCATACA TGCACCTTAA GAATATTTTA GAACAGATAA ATGCAGTCAC TGGATCACTC 
TTTACCTCGA AAGTCTTAGA AGAAAGAGCT GAAAAAGAAG CCGAAAGTGA AAGTAGTTCT 
GACACAGCAG CTGGAGATCA AATTTATGAC TGGAGTGCTG GGTTGGAAGA TACTGATGAT 
TCCCACCTCT ATTTTTCTCC AGACCATGGA AATGTGGTGT TTGCCAGTGC AATTGATGGC 
TGGGGTTTTG GCATTGAGCA TTTTGCAAAA CTGTACAGCC AAAAGATTGG AATCAAACCT 
GCAGTGCTGC TGAAGACTTT ATGGGGAGAT TATTATCTAA ACACAAAAGC AAAGAAGATA 
ATGAAAGGTG ATCAGTCAAA AGGAAAGAAG CCCTTGTTTG TGCAGTTGGT GTTGGACAAC 
ATATGGAGTC TATATGAAGC TGTTATGAAA AGAGATAAAG AAAAAATTGA GAAGATAGTT 
ACCTCATTGG GCTTGAAAAT TGGTCCTCGG GAGTCACGGC ACGCAGACCC CAAAGTTCAC 
CTCAATGCCA TTTGTAGTCA GTGGCTGCCT ATATCTGATG CAGTCCTTTC TATGGTGTGT 
AGTAAACTTC CCAGTCCCCT TGACATCACT GCAGAGAGAG TGGAAAAGCT GATGTGTGTT 
GGAGCTAAAA CATTTGATTC TTTACCACCG GAAACCCAGG AACTGAAAAG CGCTTTTCTG 
AGATGCAGTA GTGAAGAAAC GGCCCCAGTG ATTGTTTTTG TTTCCAAAAT GTTTGCTGTT 
GACGCAAAGG CATTGCCACA CAATAAACCA AGGCCTTTAA CCCAAGAAGA AATAGCTCAG 
CGACGTGAAC TTGCAAAACA GAGGCATGCA GGAAAAATGG CAGCAAGTCA AGGGAAGGAG 
GTTTCTGAAA AGCCCCCAAG TGAAAATTCA TCTGAAGGGA CTGGAAGCAC TGGTGATAAG 
AAAGGTGAAG AGCAACAGCT CACTGATCAG ATGGACGTCG TGACCTCAAA GGCAGCCAAA 
CAGGAAGCAG ACAACAAGGA GTCTTTCATT GCTTTTGCTA GAGTGTTCAG CGGTGTGGTA 
CGAAGAGGGC AAAAGATCTT TGTTTTGGGA CCCAAATATG ATCCTGCTGA GTCTCTGCAT 
AAGCTGTCAC CCCAGTGTTC TGCTACAGAT GATCTGCCAT CAGTACCTCA TATGTCTTGC 
TGTACATTGG AGAATCTATA CTTGCTCATG GGAAGAGAAC TTGAAGAGCT TGAAGAAGTA 
CCTGCAGGAA ATGTTCTAGG AATAGGAGGC CTGCAAGATT TTGTTCTAAA GTCAGCAACT 
TTGTCAACGT CACCAGCTTG CCCACCTTTT ACACCTCTCA ACTTTGAGGC TACACCAATT 
GTACGGGTAG CTGTTGAACC AAAACATCCA AGTGATATGC CTCAGCTGGT GAGAGGAATG 
AAGCTTTTAA ACCAAGCTGA TCCGTGTGTC CAAGTTTTGA TCCAGGAGAC AGGAGAGCAT 
GTACTTGTGA CAGCAGGAGA AGTTCATCTG CAGCGTTGCT TGGATGACCT GAAGGAAAGG 
TTTGCAAAGA TAGAGATTAG TGTGTCAGCA CCTATAATTC CATTTCGAGA AACAATAACG 
AGACCTCCAA AAGTCGACAT GGTAAATGAA GAAATAGGAA AACAGCAGAA AGTTGCTGTC 
ATACACCAAA CAAAAGAGGA CCAAAATAAA ATTCCTGAAG GAATTCAGGT CGATTCAGAT 
GGGCTTGTTA CAATATCTAC TCCAAATAAA CAAGCTACTC TCAGTGTAAG AGCATTACCA 
CTTCCTGAGG AGGTAACTCG ACTTCTTGAA GAAAACAGTG ACTTAATTCG AACTATGGAG 
CAACTCAGCA CGTCTCTGAA TGAAGATAAA AATGCTCATG AAATAAATCA GAAGACTATG 
GACAAAATAA AGGAATTCAA ACAAAAATTA GAGCAAAATC TGCAGGGAAG GAAGTGGAGA 
AATGCAGTTG ATCATATCTG GTCATTTGGA CCACGGAAAT GTGGGCCTAA TATTTTGTTG 
CATAAATTTG AGGGTTATAA AAATTCTGTG TGGCAGTGCC TTGAGAATAC TGAGAAAGAA 
GTAAGTAAGT ATGGAGATTT TGACAACAGC ATAGTAAGTG GATTTCAGCT GGCTACGCTT 
TCAGGTCCCA TGTGTGAAGA ACCCCTCATG GGTGTGTGTT TTACAGTGGA AAAATGGGAA 
ATGAATAAGG TTGGAGAGAC GCTTCTAACT AAAAGTCGGG ATTCAGGGGA ATGTGGTGCT 
GTAGAACATC AAGAAAGTGA AGATACTGTT TCTCCAACAA GCAATTGCAC TGTTGACACT 
TGCAGTGAGA ATGAACACGA ACAGAGCAGT CAAAGTGTTC CAAAGTCACT TGAAAAAAAT 
AGTAAAAACA AGGGAGAAGT TTTACTTACA GATTGTTATG GTCCCTTCTC TGGACAGCTG 
ATTGCCACCA TGAAGGAAGC ATGTCGTTAT GCTTTGCAAG CAAAACCACA GAGGCTTATG 
GCAGCAATGT ATACCTGCGA AATTATGGCT ACTGCGGAAG TTCTAGGTCG TGTGTATGCC 
GTCTTGTCCA AAAGAGAAGG CAGAGTGTTA CAAGAGGAGA TGAAAGAAGG AACGGATGTG 
TTCATTATTA AGGCAGTCCT GCCAGTAGCA GAAAGCTTTG GTTTTGCTGA TGAAATCAGG 
AAGAGAACTA GTGGCCTGGC CAGTCCGCAG CTGGTGTTCA GCCACTGGGA GATCATTTCA 
AGTGATCCAT TCTGGGTACC AACCACCGAA GAAGAATATT TGCACTTTGG GGAAAAGGCA 
GACTCTGAGA ACCAGGCCCG CAAGTACATG AATGCAGTGA GAAAGCGGAA GGGACTTTAT 
GTAGAAGAAA AAATTGTGGA GCATGCTGAA AAGCAAAGAA CCCTCAGTAG GAATAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.
Genome biology6(1)R6(2005)
Caldwell RB,Kierzek AM,Arakawa H,Bezzubov Y,Zaim J,Fiedler P,Kutter S,Blagodatski A,Kostovska D,Koter M,Plachy J,Carninci P,Hayashizaki Y,Buerstedde JM

A comprehensive collection of chicken cDNAs.
Current biology : CB12(22)1965-9(2002 Nov)
Boardman PE,Sanz-Ezquerro J,Overton IM,Burt DW,Bosch E,Fong WT,Tickle C,Brown WR,Wilson SA,Hubbard SJ

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EFL1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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