EFL1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	EFL1
Entrez Gene ID	479061
Full Name	elongation factor like GTPase 1
General protein information	Preferred Names elongation factor like GTPase 1 Names elongation factor-like GTPase 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_845018.4	XP_850111.1	elongation factor Tu GTP-binding domain-containing protein 1
XM_845018.5	XP_850111.1	elongation factor-like GTPase 1

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os03g0650700	NP_001050786.1
Magnaporthe oryzae (rice blast fungus)	MGG_05265	XP_003712823.1
Schizosaccharomyces pombe (fission yeast)	SPCC553.08c	NP_587766.1
Drosophila melanogaster (fruit fly)	CG33158	NP_788515.1
Arabidopsis thaliana (thale cress)	AT3G22980	NP_188938.1
Eremothecium gossypii	AGOS_AFR031C	NP_985578.2
Bos taurus (cattle)	EFTUD1	XP_002696652.1
Danio rerio (zebrafish)	eftud1	XP_697184.5
Saccharomyces cerevisiae (baker's yeast)	RIA1	NP_014236.1
Pan troglodytes (chimpanzee)	EFTUD1	XP_510546.2
Macaca mulatta (Rhesus monkey)	EFTUD1	XP_002804960.1
Mus musculus (house mouse)	Eftud1	NP_780526.2
Rattus norvegicus (Norway rat)	Eftud1	NP_001101004.1
Gallus gallus (chicken)	EFTUD1	NP_001034345.2
Kluyveromyces lactis	KLLA0D07898g	XP_453415.1
Oryza sativa (rice)	Os02g0543300	NP_001047074.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003001	XP_311881.3
Homo sapiens (human)	EFTUD1	NP_078856.4
Neurospora crassa	NCU06278	XP_962882.2
Canis lupus familiaris (dog)	EFTUD1	XP_850111.1
Xenopus tropicalis (tropical clawed frog)	eftud1	XP_002940146.2

Gene Ontology

The following EFL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EFL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe04340	XM_845018.5 Latest version!	Canis lupus familiaris elongation factor like GTPase 1 (EFL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCe04340	XM_845018.4	Canis lupus familiaris elongation factor Tu GTP binding domain containing 1 (EFTUD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EFL1 ( XM_845018.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_850111.1 Canis lupus familiaris elongation factor Tu GTP binding domain containing 1 (EFTUD1), mRNA.

Price & Availability↑

CloneID	OCe04340
Clone ID Related Accession (Same CDS sequence)	XM_845018.4 , XM_845018.5
Accession Version	XM_845018.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	elongation factor Tu GTP-binding domain-containing protein 1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_845018.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTCA ATAGTTTGGA GAAGATGATT CACCTGCAGA AAAACACTGC TAACATCAGG 
AACATCTGCG TGTTAGCTCA CGTCGACCAC GGAAAAACTA CTCTAGCTGA CTGTCTTATA 
TCCAGCAATG GAATCATTTC TAGCCGCCTC GCAGGCAAGT TAAGGTACAT GGACAGCAGA 
GAAGATGAAC AGGTTCGAGG GATCACTATG AAATCCAGTG CCATTTCACT ACATTATGCA 
AAAGATAATG AGGATTACCT GATCAATCTC ATAGACTCTC CGGGGCACGT GGACTTCTCC 
TCAGAAGTGT CAACAGCTGT GCGCATCTGT GACGGGTGTA TCATCGTGGT CGATGCCGTG 
GAAGGAGTGT GTCCACAGAC ACAAGCTGTT CTGCGACAAG CCTGGCTCGA AAACATCCGC 
CCGGTTTTGG TGATTAATAA GCTAGATCGT TTGATAGTGG AACTGAAATT TACCCCCCAG 
GAGGCCTATT CTCACCTCAA GAATATTTTA GAGCAGATTA ATGCACTCAC AGGAACTCTT 
TTTACTTCTA AAGTCCTAGA AGAAAGAGCA GAGAGAGAGA CTGAATCGCA GGTGAATCCC 
AGTTCTGAAC CAGGAGAGCA AGTATACGAC TGGAGCACTG GCTTGGAAGA CACGGATGAT 
TCTCACCTCT ACTTCTCTCC AGATCAGGGC AATGTGGTGT TTACAAGTGC ATTAGATGGG 
TGGGGCTTTG GGGTTGAGCA CTTTGCCACA ATCTACAGTC AAAAAATTGG CATCAAAAAG 
GAAGTTCTTC TGAAAACCTT GTGGGGAGAT TATTATATCA ATATGAAGGC TAAGAAGATC 
ATGAAGGTTG ATCAGGCAAA AGGAAAGAAA CCTTTATTTG TGCAGCTGAT CCTGGAGAAT 
ATATGGAGTT TGTATGATGC CGTCTTGAAA AAGGACAGAG AAAAAATTGA TAAAATAGTG 
ACTTCTTTAG GATTGAAAAT TGGAGCCCGA GAGGCACGAC ATTCAGATCC TAAAGTTCAG 
ATCAATGCCA TCTGCAGCCA GTGGCTGCCC ATCTCCCATG CTGTGCTCGC TATGGTATGC 
CAGAAGCTTC CCAGCCCCCT GGATATGACA GCCGAGAGGG TGGAGAGACT GATGTGCACA 
GGATCACAGA CGTTTGACTC TCTACCGCCG GAAACCCAGG CCCTGAAAGC AGCTTTCCTG 
AAATGTGGAA GCGAGGACAC TGCTCCAGTT ATTATCTTTG TTTCCAAAAT GTTTGCAGTT 
GATGCCAAGG CTCTGCCCCA GAATAAACCC AGACCTCTCA CTCAGGAAGA AATTGCTCAG 
AGGCGTGAGC GTGCAAAGCA GAGGCACGCG GAGAAGCTGG CAGCAGCGCA GGGCCAGGCA 
CCCATGGAGC GCCCCCAGGG TGGGAGTGCC CTTGAAACAA GTCCACAGGC AGAAGAGCCA 
AAAGGCGATG AGCATCAGCT GGAGACTGTG ACCCTTAAAT CTCTGCCCCA GGAAGGAAAC 
AACCAAGAGT CTTTTATTGC ATTTGCTCGC GTGTTCAGTG GTGTGGCTCG AAGAGGAAAG 
AAAATTTTTG TCTTGGGGCC TAAATACAGT CCCGTTGAGT TTTTGCAGCG GGTGCCATTA 
GGCTTCTCGG CTCCTCTAGA GGACCTTCCC CCGGTTCCCC ACATGGCATG CTGCACTCTA 
GAAAACCTGT ATCTTCTGAT GGGGAGAGAA CTGGAAGACC TAGAGGAGGT ACCTCCAGGG 
AATGTGCTAG GCATCGGGGG CCTTCAGGAC TTGGTGCTGA AGTCAGCAAC ACTGTGCAGT 
GTGCCATCTT GCCCGCCGTT CATACCGCTC AACTTCGAGG CCACCCCTAT TGTGAGGGTT 
GCTGTTGAAC CGAAACACCC AAGTGAAATG CCTCAGCTCG TCAGAGGAAT GAAACTCTTA 
AACCAGGCTG ACCCCTGTGT CCAGATCTTA ATTCAAGAAA CAGGAGAGCA CGTCTTAGTG 
ACAGCAGGGG AGGTCCACCT TCAGCGGTGC CTGGATGACT TGAAAGAAAG GTTTGCTAGG 
ATTCAAATCA GTGTATCTGA ACCCATTATT CCATTCAGAG AAACAATCAC CAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATAGGC AAACAACAAA AAGTTGCAGT TATCCACCAA 
ACAAAAGAAG ATCAGAGCAA AATCCCTGAA GGAATCCAAG TTGACTCTGA CGGGCTCATC 
ACCATAACAA CCCCCAATAA GCTTGCCACA CTCAGTGTTC GAGCCATGCC CCTTCCAGAA 
GAAGTCACAC AGATCCTGGA AGAAAATAGT GATTTGATTC GTTCTATGGA GCAGTTGACA 
TCCTCTTTGA ATGAGGGCAG ACATAATCAG ATGATGCACC AGAAGACCCA AGAAAAAATG 
TGGGAATTCA AAGGGAGACT AGAGCAGCAC CTAACAGGGA GGAAATGGAG GAACACTGTT 
GACCAGATCT GGTCATTTGG CCCAAGGAAA TGCGGGCCCA ACATATTAGT AAATAAGAGT 
GAAGACTTTC AGAACTCAGT ATGGACAGGC CCAGCCATCA AAGCTTCAAA AGAGACCAGT 
AGATACCGAG ATTTTGGCAA TAGCATCGTG AGTGGTTTCC AGCTAGCAAC CCTCTCTGGC 
CCCATGTGTG AGGAGCCTCT CATGGGTGTC TGTTTCGTTC TGGAAAAATG GGACCTAAGT 
AAATTTGAGG AACAAGGGGC GAGTGATAAA CACAATCCAG AGCAAAGAGA CTTGGTGCAG 
GAGGGACAGG GGGGAGGAGA AGCCTGTTCC AGCAAAGGTG AAAACCAGGG ACTCCAAGAT 
GGCAGCCTTG AGCCCATGGA GAAGAAGACT TCCCAGAGAG GAGAGTCTTC ACTCACTGAC 
TGCTACGGAC CCTTCTCAGG ACAGCTCATC GCCACCATGA AGGAAGCCTG TCGCTATGCC 
CTGCAGGTGA AACCTCAGCG TCTGATGGCA GCAATGTACA CGTGTGACAT CATGGCCACC 
GGTGATGTTC TTGGTCGTGT CTATGCCGTC TTGTCAAAGA GAGAAGGTCG AGTGCTTCAA 
GAAGAAATGA AAGAAGGGAC AGACATGTTC ATCATCAAGG CGGTGCTGCC CGTTGCTGAG 
AGCTTCGGCT TTGCGGATGA GATCAGGAAG CGGACGAGTG GCTTGGCCAG CCCGCAGCTG 
GTGTTCAGCC ACTGGGAGAT CATTCCCAGC GACCCCTTCT GGGTGCCAAC CACCGAGGAA 
GAATACTTGC ACTTTGGGGA AAAGGCTGAT TCTGAGAACC AGGCCCGGAA GTACATGAAC 
GCCGTGCGCA AACGGAAGGG TCTTTACGTG GAAGAGAAGA TCGTGGAGCA CGCAGAAAAG 
CAGAGAACCC TCAGCAAAAA TAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_850111.1
CDS	71..3457
Translation	MVLNSLEKMIHLQKNTANIRNICVLAHVDHGKTTLADCLISSNGIISSRLAGKLRYMDSREDEQVRGITMKSSAISLHYAKDNEDYLINLIDSPGHVDFSSEVSTAVRICDGCIIVVDAVEGVCPQTQAVLRQAWLENIRPVLVINKLDRLIVELKFTPQEAYSHLKNILEQINALTGTLFTSKVLEERAERETESQVNPSSEPGEQVYDWSTGLEDTDDSHLYFSPDQGNVVFTSALDGWGFGVEHFATIYSQKIGIKKEVLLKTLWGDYYINMKAKKIMKVDQAKGKKPLFVQLILENIWSLYDAVLKKDREKIDKIVTSLGLKIGAREARHSDPKVQINAICSQWLPISHAVLAMVCQKLPSPLDMTAERVERLMCTGSQTFDSLPPETQALKAAFLKCGSEDTAPVIIFVSKMFAVDAKALPQNKPRPLTQEEIAQRRERAKQRHAEKLAAAQGQAPMERPQGGSALETSPQAEEPKGDEHQLETVTLKSLPQEGNNQESFIAFARVFSGVARRGKKIFVLGPKYSPVEFLQRVPLGFSAPLEDLPPVPHMACCTLENLYLLMGRELEDLEEVPPGNVLGIGGLQDLVLKSATLCSVPSCPPFIPLNFEATPIVRVAVEPKHPSEMPQLVRGMKLLNQADPCVQILIQETGEHVLVTAGEVHLQRCLDDLKERFARIQISVSEPIIPFRETITKPPKVDMVNEEIGKQQKVAVIHQTKEDQSKIPEGIQVDSDGLITITTPNKLATLSVRAMPLPEEVTQILEENSDLIRSMEQLTSSLNEGRHNQMMHQKTQEKMWEFKGRLEQHLTGRKWRNTVDQIWSFGPRKCGPNILVNKSEDFQNSVWTGPAIKASKETSRYRDFGNSIVSGFQLATLSGPMCEEPLMGVCFVLEKWDLSKFEEQGASDKHNPEQRDLVQEGQGGGEACSSKGENQGLQDGSLEPMEKKTSQRGESSLTDCYGPFSGQLIATMKEACRYALQVKPQRLMAAMYTCDIMATGDVLGRVYAVLSKREGRVLQEEMKEGTDMFIIKAVLPVAESFGFADEIRKRTSGLASPQLVFSHWEIIPSDPFWVPTTEEEYLHFGEKADSENQARKYMNAVRKRKGLYVEEKIVEHAEKQRTLSKNK

Target ORF information:

RefSeq Version	XM_845018.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris elongation factor Tu GTP binding domain containing 1 (EFTUD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_845018.4

ORF Insert Sequence:

ATGGTGCTCA ATAGTTTGGA GAAGATGATT CACCTGCAGA AAAACACTGC TAACATCAGG 
AACATCTGCG TGTTAGCTCA CGTCGACCAC GGAAAAACTA CTCTAGCTGA CTGTCTTATA 
TCCAGCAATG GAATCATTTC TAGCCGCCTC GCAGGCAAGT TAAGGTACAT GGACAGCAGA 
GAAGATGAAC AGGTTCGAGG GATCACTATG AAATCCAGTG CCATTTCACT ACATTATGCA 
AAAGATAATG AGGATTACCT GATCAATCTC ATAGACTCTC CGGGGCACGT GGACTTCTCC 
TCAGAAGTGT CAACAGCTGT GCGCATCTGT GACGGGTGTA TCATCGTGGT CGATGCCGTG 
GAAGGAGTGT GTCCACAGAC ACAAGCTGTT CTGCGACAAG CCTGGCTCGA AAACATCCGC 
CCGGTTTTGG TGATTAATAA GCTAGATCGT TTGATAGTGG AACTGAAATT TACCCCCCAG 
GAGGCCTATT CTCACCTCAA GAATATTTTA GAGCAGATTA ATGCACTCAC AGGAACTCTT 
TTTACTTCTA AAGTCCTAGA AGAAAGAGCA GAGAGAGAGA CTGAATCGCA GGTGAATCCC 
AGTTCTGAAC CAGGAGAGCA AGTATACGAC TGGAGCACTG GCTTGGAAGA CACGGATGAT 
TCTCACCTCT ACTTCTCTCC AGATCAGGGC AATGTGGTGT TTACAAGTGC ATTAGATGGG 
TGGGGCTTTG GGGTTGAGCA CTTTGCCACA ATCTACAGTC AAAAAATTGG CATCAAAAAG 
GAAGTTCTTC TGAAAACCTT GTGGGGAGAT TATTATATCA ATATGAAGGC TAAGAAGATC 
ATGAAGGTTG ATCAGGCAAA AGGAAAGAAA CCTTTATTTG TGCAGCTGAT CCTGGAGAAT 
ATATGGAGTT TGTATGATGC CGTCTTGAAA AAGGACAGAG AAAAAATTGA TAAAATAGTG 
ACTTCTTTAG GATTGAAAAT TGGAGCCCGA GAGGCACGAC ATTCAGATCC TAAAGTTCAG 
ATCAATGCCA TCTGCAGCCA GTGGCTGCCC ATCTCCCATG CTGTGCTCGC TATGGTATGC 
CAGAAGCTTC CCAGCCCCCT GGATATGACA GCCGAGAGGG TGGAGAGACT GATGTGCACA 
GGATCACAGA CGTTTGACTC TCTACCGCCG GAAACCCAGG CCCTGAAAGC AGCTTTCCTG 
AAATGTGGAA GCGAGGACAC TGCTCCAGTT ATTATCTTTG TTTCCAAAAT GTTTGCAGTT 
GATGCCAAGG CTCTGCCCCA GAATAAACCC AGACCTCTCA CTCAGGAAGA AATTGCTCAG 
AGGCGTGAGC GTGCAAAGCA GAGGCACGCG GAGAAGCTGG CAGCAGCGCA GGGCCAGGCA 
CCCATGGAGC GCCCCCAGGG TGGGAGTGCC CTTGAAACAA GTCCACAGGC AGAAGAGCCA 
AAAGGCGATG AGCATCAGCT GGAGACTGTG ACCCTTAAAT CTCTGCCCCA GGAAGGAAAC 
AACCAAGAGT CTTTTATTGC ATTTGCTCGC GTGTTCAGTG GTGTGGCTCG AAGAGGAAAG 
AAAATTTTTG TCTTGGGGCC TAAATACAGT CCCGTTGAGT TTTTGCAGCG GGTGCCATTA 
GGCTTCTCGG CTCCTCTAGA GGACCTTCCC CCGGTTCCCC ACATGGCATG CTGCACTCTA 
GAAAACCTGT ATCTTCTGAT GGGGAGAGAA CTGGAAGACC TAGAGGAGGT ACCTCCAGGG 
AATGTGCTAG GCATCGGGGG CCTTCAGGAC TTGGTGCTGA AGTCAGCAAC ACTGTGCAGT 
GTGCCATCTT GCCCGCCGTT CATACCGCTC AACTTCGAGG CCACCCCTAT TGTGAGGGTT 
GCTGTTGAAC CGAAACACCC AAGTGAAATG CCTCAGCTCG TCAGAGGAAT GAAACTCTTA 
AACCAGGCTG ACCCCTGTGT CCAGATCTTA ATTCAAGAAA CAGGAGAGCA CGTCTTAGTG 
ACAGCAGGGG AGGTCCACCT TCAGCGGTGC CTGGATGACT TGAAAGAAAG GTTTGCTAGG 
ATTCAAATCA GTGTATCTGA ACCCATTATT CCATTCAGAG AAACAATCAC CAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATAGGC AAACAACAAA AAGTTGCAGT TATCCACCAA 
ACAAAAGAAG ATCAGAGCAA AATCCCTGAA GGAATCCAAG TTGACTCTGA CGGGCTCATC 
ACCATAACAA CCCCCAATAA GCTTGCCACA CTCAGTGTTC GAGCCATGCC CCTTCCAGAA 
GAAGTCACAC AGATCCTGGA AGAAAATAGT GATTTGATTC GTTCTATGGA GCAGTTGACA 
TCCTCTTTGA ATGAGGGCAG ACATAATCAG ATGATGCACC AGAAGACCCA AGAAAAAATG 
TGGGAATTCA AAGGGAGACT AGAGCAGCAC CTAACAGGGA GGAAATGGAG GAACACTGTT 
GACCAGATCT GGTCATTTGG CCCAAGGAAA TGCGGGCCCA ACATATTAGT AAATAAGAGT 
GAAGACTTTC AGAACTCAGT ATGGACAGGC CCAGCCATCA AAGCTTCAAA AGAGACCAGT 
AGATACCGAG ATTTTGGCAA TAGCATCGTG AGTGGTTTCC AGCTAGCAAC CCTCTCTGGC 
CCCATGTGTG AGGAGCCTCT CATGGGTGTC TGTTTCGTTC TGGAAAAATG GGACCTAAGT 
AAATTTGAGG AACAAGGGGC GAGTGATAAA CACAATCCAG AGCAAAGAGA CTTGGTGCAG 
GAGGGACAGG GGGGAGGAGA AGCCTGTTCC AGCAAAGGTG AAAACCAGGG ACTCCAAGAT 
GGCAGCCTTG AGCCCATGGA GAAGAAGACT TCCCAGAGAG GAGAGTCTTC ACTCACTGAC 
TGCTACGGAC CCTTCTCAGG ACAGCTCATC GCCACCATGA AGGAAGCCTG TCGCTATGCC 
CTGCAGGTGA AACCTCAGCG TCTGATGGCA GCAATGTACA CGTGTGACAT CATGGCCACC 
GGTGATGTTC TTGGTCGTGT CTATGCCGTC TTGTCAAAGA GAGAAGGTCG AGTGCTTCAA 
GAAGAAATGA AAGAAGGGAC AGACATGTTC ATCATCAAGG CGGTGCTGCC CGTTGCTGAG 
AGCTTCGGCT TTGCGGATGA GATCAGGAAG CGGACGAGTG GCTTGGCCAG CCCGCAGCTG 
GTGTTCAGCC ACTGGGAGAT CATTCCCAGC GACCCCTTCT GGGTGCCAAC CACCGAGGAA 
GAATACTTGC ACTTTGGGGA AAAGGCTGAT TCTGAGAACC AGGCCCGGAA GTACATGAAC 
GCCGTGCGCA AACGGAAGGG TCTTTACGTG GAAGAGAAGA TCGTGGAGCA CGCAGAAAAG 
CAGAGAACCC TCAGCAAAAA TAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFL1 ( XM_845018.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_850111.1 Canis lupus familiaris elongation factor like GTPase 1 (EFL1), mRNA.

Price & Availability↑

CloneID	OCe04340
Clone ID Related Accession (Same CDS sequence)	XM_845018.4 , XM_845018.5
Accession Version	XM_845018.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	elongation factor-like GTPase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_845018.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTCA ATAGTTTGGA GAAGATGATT CACCTGCAGA AAAACACTGC TAACATCAGG 
AACATCTGCG TGTTAGCTCA CGTCGACCAC GGAAAAACTA CTCTAGCTGA CTGTCTTATA 
TCCAGCAATG GAATCATTTC TAGCCGCCTC GCAGGCAAGT TAAGGTACAT GGACAGCAGA 
GAAGATGAAC AGGTTCGAGG GATCACTATG AAATCCAGTG CCATTTCACT ACATTATGCA 
AAAGATAATG AGGATTACCT GATCAATCTC ATAGACTCTC CGGGGCACGT GGACTTCTCC 
TCAGAAGTGT CAACAGCTGT GCGCATCTGT GACGGGTGTA TCATCGTGGT CGATGCCGTG 
GAAGGAGTGT GTCCACAGAC ACAAGCTGTT CTGCGACAAG CCTGGCTCGA AAACATCCGC 
CCGGTTTTGG TGATTAATAA GCTAGATCGT TTGATAGTGG AACTGAAATT TACCCCCCAG 
GAGGCCTATT CTCACCTCAA GAATATTTTA GAGCAGATTA ATGCACTCAC AGGAACTCTT 
TTTACTTCTA AAGTCCTAGA AGAAAGAGCA GAGAGAGAGA CTGAATCGCA GGTGAATCCC 
AGTTCTGAAC CAGGAGAGCA AGTATACGAC TGGAGCACTG GCTTGGAAGA CACGGATGAT 
TCTCACCTCT ACTTCTCTCC AGATCAGGGC AATGTGGTGT TTACAAGTGC ATTAGATGGG 
TGGGGCTTTG GGGTTGAGCA CTTTGCCACA ATCTACAGTC AAAAAATTGG CATCAAAAAG 
GAAGTTCTTC TGAAAACCTT GTGGGGAGAT TATTATATCA ATATGAAGGC TAAGAAGATC 
ATGAAGGTTG ATCAGGCAAA AGGAAAGAAA CCTTTATTTG TGCAGCTGAT CCTGGAGAAT 
ATATGGAGTT TGTATGATGC CGTCTTGAAA AAGGACAGAG AAAAAATTGA TAAAATAGTG 
ACTTCTTTAG GATTGAAAAT TGGAGCCCGA GAGGCACGAC ATTCAGATCC TAAAGTTCAG 
ATCAATGCCA TCTGCAGCCA GTGGCTGCCC ATCTCCCATG CTGTGCTCGC TATGGTATGC 
CAGAAGCTTC CCAGCCCCCT GGATATGACA GCCGAGAGGG TGGAGAGACT GATGTGCACA 
GGATCACAGA CGTTTGACTC TCTACCGCCG GAAACCCAGG CCCTGAAAGC AGCTTTCCTG 
AAATGTGGAA GCGAGGACAC TGCTCCAGTT ATTATCTTTG TTTCCAAAAT GTTTGCAGTT 
GATGCCAAGG CTCTGCCCCA GAATAAACCC AGACCTCTCA CTCAGGAAGA AATTGCTCAG 
AGGCGTGAGC GTGCAAAGCA GAGGCACGCG GAGAAGCTGG CAGCAGCGCA GGGCCAGGCA 
CCCATGGAGC GCCCCCAGGG TGGGAGTGCC CTTGAAACAA GTCCACAGGC AGAAGAGCCA 
AAAGGCGATG AGCATCAGCT GGAGACTGTG ACCCTTAAAT CTCTGCCCCA GGAAGGAAAC 
AACCAAGAGT CTTTTATTGC ATTTGCTCGC GTGTTCAGTG GTGTGGCTCG AAGAGGAAAG 
AAAATTTTTG TCTTGGGGCC TAAATACAGT CCCGTTGAGT TTTTGCAGCG GGTGCCATTA 
GGCTTCTCGG CTCCTCTAGA GGACCTTCCC CCGGTTCCCC ACATGGCATG CTGCACTCTA 
GAAAACCTGT ATCTTCTGAT GGGGAGAGAA CTGGAAGACC TAGAGGAGGT ACCTCCAGGG 
AATGTGCTAG GCATCGGGGG CCTTCAGGAC TTGGTGCTGA AGTCAGCAAC ACTGTGCAGT 
GTGCCATCTT GCCCGCCGTT CATACCGCTC AACTTCGAGG CCACCCCTAT TGTGAGGGTT 
GCTGTTGAAC CGAAACACCC AAGTGAAATG CCTCAGCTCG TCAGAGGAAT GAAACTCTTA 
AACCAGGCTG ACCCCTGTGT CCAGATCTTA ATTCAAGAAA CAGGAGAGCA CGTCTTAGTG 
ACAGCAGGGG AGGTCCACCT TCAGCGGTGC CTGGATGACT TGAAAGAAAG GTTTGCTAGG 
ATTCAAATCA GTGTATCTGA ACCCATTATT CCATTCAGAG AAACAATCAC CAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATAGGC AAACAACAAA AAGTTGCAGT TATCCACCAA 
ACAAAAGAAG ATCAGAGCAA AATCCCTGAA GGAATCCAAG TTGACTCTGA CGGGCTCATC 
ACCATAACAA CCCCCAATAA GCTTGCCACA CTCAGTGTTC GAGCCATGCC CCTTCCAGAA 
GAAGTCACAC AGATCCTGGA AGAAAATAGT GATTTGATTC GTTCTATGGA GCAGTTGACA 
TCCTCTTTGA ATGAGGGCAG ACATAATCAG ATGATGCACC AGAAGACCCA AGAAAAAATG 
TGGGAATTCA AAGGGAGACT AGAGCAGCAC CTAACAGGGA GGAAATGGAG GAACACTGTT 
GACCAGATCT GGTCATTTGG CCCAAGGAAA TGCGGGCCCA ACATATTAGT AAATAAGAGT 
GAAGACTTTC AGAACTCAGT ATGGACAGGC CCAGCCATCA AAGCTTCAAA AGAGACCAGT 
AGATACCGAG ATTTTGGCAA TAGCATCGTG AGTGGTTTCC AGCTAGCAAC CCTCTCTGGC 
CCCATGTGTG AGGAGCCTCT CATGGGTGTC TGTTTCGTTC TGGAAAAATG GGACCTAAGT 
AAATTTGAGG AACAAGGGGC GAGTGATAAA CACAATCCAG AGCAAAGAGA CTTGGTGCAG 
GAGGGACAGG GGGGAGGAGA AGCCTGTTCC AGCAAAGGTG AAAACCAGGG ACTCCAAGAT 
GGCAGCCTTG AGCCCATGGA GAAGAAGACT TCCCAGAGAG GAGAGTCTTC ACTCACTGAC 
TGCTACGGAC CCTTCTCAGG ACAGCTCATC GCCACCATGA AGGAAGCCTG TCGCTATGCC 
CTGCAGGTGA AACCTCAGCG TCTGATGGCA GCAATGTACA CGTGTGACAT CATGGCCACC 
GGTGATGTTC TTGGTCGTGT CTATGCCGTC TTGTCAAAGA GAGAAGGTCG AGTGCTTCAA 
GAAGAAATGA AAGAAGGGAC AGACATGTTC ATCATCAAGG CGGTGCTGCC CGTTGCTGAG 
AGCTTCGGCT TTGCGGATGA GATCAGGAAG CGGACGAGTG GCTTGGCCAG CCCGCAGCTG 
GTGTTCAGCC ACTGGGAGAT CATTCCCAGC GACCCCTTCT GGGTGCCAAC CACCGAGGAA 
GAATACTTGC ACTTTGGGGA AAAGGCTGAT TCTGAGAACC AGGCCCGGAA GTACATGAAC 
GCCGTGCGCA AACGGAAGGG TCTTTACGTG GAAGAGAAGA TCGTGGAGCA CGCAGAAAAG 
CAGAGAACCC TCAGCAAAAA TAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_850111.1
CDS	65..3451
Translation	MVLNSLEKMIHLQKNTANIRNICVLAHVDHGKTTLADCLISSNGIISSRLAGKLRYMDSREDEQVRGITMKSSAISLHYAKDNEDYLINLIDSPGHVDFSSEVSTAVRICDGCIIVVDAVEGVCPQTQAVLRQAWLENIRPVLVINKLDRLIVELKFTPQEAYSHLKNILEQINALTGTLFTSKVLEERAERETESQVNPSSEPGEQVYDWSTGLEDTDDSHLYFSPDQGNVVFTSALDGWGFGVEHFATIYSQKIGIKKEVLLKTLWGDYYINMKAKKIMKVDQAKGKKPLFVQLILENIWSLYDAVLKKDREKIDKIVTSLGLKIGAREARHSDPKVQINAICSQWLPISHAVLAMVCQKLPSPLDMTAERVERLMCTGSQTFDSLPPETQALKAAFLKCGSEDTAPVIIFVSKMFAVDAKALPQNKPRPLTQEEIAQRRERAKQRHAEKLAAAQGQAPMERPQGGSALETSPQAEEPKGDEHQLETVTLKSLPQEGNNQESFIAFARVFSGVARRGKKIFVLGPKYSPVEFLQRVPLGFSAPLEDLPPVPHMACCTLENLYLLMGRELEDLEEVPPGNVLGIGGLQDLVLKSATLCSVPSCPPFIPLNFEATPIVRVAVEPKHPSEMPQLVRGMKLLNQADPCVQILIQETGEHVLVTAGEVHLQRCLDDLKERFARIQISVSEPIIPFRETITKPPKVDMVNEEIGKQQKVAVIHQTKEDQSKIPEGIQVDSDGLITITTPNKLATLSVRAMPLPEEVTQILEENSDLIRSMEQLTSSLNEGRHNQMMHQKTQEKMWEFKGRLEQHLTGRKWRNTVDQIWSFGPRKCGPNILVNKSEDFQNSVWTGPAIKASKETSRYRDFGNSIVSGFQLATLSGPMCEEPLMGVCFVLEKWDLSKFEEQGASDKHNPEQRDLVQEGQGGGEACSSKGENQGLQDGSLEPMEKKTSQRGESSLTDCYGPFSGQLIATMKEACRYALQVKPQRLMAAMYTCDIMATGDVLGRVYAVLSKREGRVLQEEMKEGTDMFIIKAVLPVAESFGFADEIRKRTSGLASPQLVFSHWEIIPSDPFWVPTTEEEYLHFGEKADSENQARKYMNAVRKRKGLYVEEKIVEHAEKQRTLSKNK

Target ORF information:

RefSeq Version	XM_845018.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris elongation factor like GTPase 1 (EFL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_845018.5

ORF Insert Sequence:

ATGGTGCTCA ATAGTTTGGA GAAGATGATT CACCTGCAGA AAAACACTGC TAACATCAGG 
AACATCTGCG TGTTAGCTCA CGTCGACCAC GGAAAAACTA CTCTAGCTGA CTGTCTTATA 
TCCAGCAATG GAATCATTTC TAGCCGCCTC GCAGGCAAGT TAAGGTACAT GGACAGCAGA 
GAAGATGAAC AGGTTCGAGG GATCACTATG AAATCCAGTG CCATTTCACT ACATTATGCA 
AAAGATAATG AGGATTACCT GATCAATCTC ATAGACTCTC CGGGGCACGT GGACTTCTCC 
TCAGAAGTGT CAACAGCTGT GCGCATCTGT GACGGGTGTA TCATCGTGGT CGATGCCGTG 
GAAGGAGTGT GTCCACAGAC ACAAGCTGTT CTGCGACAAG CCTGGCTCGA AAACATCCGC 
CCGGTTTTGG TGATTAATAA GCTAGATCGT TTGATAGTGG AACTGAAATT TACCCCCCAG 
GAGGCCTATT CTCACCTCAA GAATATTTTA GAGCAGATTA ATGCACTCAC AGGAACTCTT 
TTTACTTCTA AAGTCCTAGA AGAAAGAGCA GAGAGAGAGA CTGAATCGCA GGTGAATCCC 
AGTTCTGAAC CAGGAGAGCA AGTATACGAC TGGAGCACTG GCTTGGAAGA CACGGATGAT 
TCTCACCTCT ACTTCTCTCC AGATCAGGGC AATGTGGTGT TTACAAGTGC ATTAGATGGG 
TGGGGCTTTG GGGTTGAGCA CTTTGCCACA ATCTACAGTC AAAAAATTGG CATCAAAAAG 
GAAGTTCTTC TGAAAACCTT GTGGGGAGAT TATTATATCA ATATGAAGGC TAAGAAGATC 
ATGAAGGTTG ATCAGGCAAA AGGAAAGAAA CCTTTATTTG TGCAGCTGAT CCTGGAGAAT 
ATATGGAGTT TGTATGATGC CGTCTTGAAA AAGGACAGAG AAAAAATTGA TAAAATAGTG 
ACTTCTTTAG GATTGAAAAT TGGAGCCCGA GAGGCACGAC ATTCAGATCC TAAAGTTCAG 
ATCAATGCCA TCTGCAGCCA GTGGCTGCCC ATCTCCCATG CTGTGCTCGC TATGGTATGC 
CAGAAGCTTC CCAGCCCCCT GGATATGACA GCCGAGAGGG TGGAGAGACT GATGTGCACA 
GGATCACAGA CGTTTGACTC TCTACCGCCG GAAACCCAGG CCCTGAAAGC AGCTTTCCTG 
AAATGTGGAA GCGAGGACAC TGCTCCAGTT ATTATCTTTG TTTCCAAAAT GTTTGCAGTT 
GATGCCAAGG CTCTGCCCCA GAATAAACCC AGACCTCTCA CTCAGGAAGA AATTGCTCAG 
AGGCGTGAGC GTGCAAAGCA GAGGCACGCG GAGAAGCTGG CAGCAGCGCA GGGCCAGGCA 
CCCATGGAGC GCCCCCAGGG TGGGAGTGCC CTTGAAACAA GTCCACAGGC AGAAGAGCCA 
AAAGGCGATG AGCATCAGCT GGAGACTGTG ACCCTTAAAT CTCTGCCCCA GGAAGGAAAC 
AACCAAGAGT CTTTTATTGC ATTTGCTCGC GTGTTCAGTG GTGTGGCTCG AAGAGGAAAG 
AAAATTTTTG TCTTGGGGCC TAAATACAGT CCCGTTGAGT TTTTGCAGCG GGTGCCATTA 
GGCTTCTCGG CTCCTCTAGA GGACCTTCCC CCGGTTCCCC ACATGGCATG CTGCACTCTA 
GAAAACCTGT ATCTTCTGAT GGGGAGAGAA CTGGAAGACC TAGAGGAGGT ACCTCCAGGG 
AATGTGCTAG GCATCGGGGG CCTTCAGGAC TTGGTGCTGA AGTCAGCAAC ACTGTGCAGT 
GTGCCATCTT GCCCGCCGTT CATACCGCTC AACTTCGAGG CCACCCCTAT TGTGAGGGTT 
GCTGTTGAAC CGAAACACCC AAGTGAAATG CCTCAGCTCG TCAGAGGAAT GAAACTCTTA 
AACCAGGCTG ACCCCTGTGT CCAGATCTTA ATTCAAGAAA CAGGAGAGCA CGTCTTAGTG 
ACAGCAGGGG AGGTCCACCT TCAGCGGTGC CTGGATGACT TGAAAGAAAG GTTTGCTAGG 
ATTCAAATCA GTGTATCTGA ACCCATTATT CCATTCAGAG AAACAATCAC CAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATAGGC AAACAACAAA AAGTTGCAGT TATCCACCAA 
ACAAAAGAAG ATCAGAGCAA AATCCCTGAA GGAATCCAAG TTGACTCTGA CGGGCTCATC 
ACCATAACAA CCCCCAATAA GCTTGCCACA CTCAGTGTTC GAGCCATGCC CCTTCCAGAA 
GAAGTCACAC AGATCCTGGA AGAAAATAGT GATTTGATTC GTTCTATGGA GCAGTTGACA 
TCCTCTTTGA ATGAGGGCAG ACATAATCAG ATGATGCACC AGAAGACCCA AGAAAAAATG 
TGGGAATTCA AAGGGAGACT AGAGCAGCAC CTAACAGGGA GGAAATGGAG GAACACTGTT 
GACCAGATCT GGTCATTTGG CCCAAGGAAA TGCGGGCCCA ACATATTAGT AAATAAGAGT 
GAAGACTTTC AGAACTCAGT ATGGACAGGC CCAGCCATCA AAGCTTCAAA AGAGACCAGT 
AGATACCGAG ATTTTGGCAA TAGCATCGTG AGTGGTTTCC AGCTAGCAAC CCTCTCTGGC 
CCCATGTGTG AGGAGCCTCT CATGGGTGTC TGTTTCGTTC TGGAAAAATG GGACCTAAGT 
AAATTTGAGG AACAAGGGGC GAGTGATAAA CACAATCCAG AGCAAAGAGA CTTGGTGCAG 
GAGGGACAGG GGGGAGGAGA AGCCTGTTCC AGCAAAGGTG AAAACCAGGG ACTCCAAGAT 
GGCAGCCTTG AGCCCATGGA GAAGAAGACT TCCCAGAGAG GAGAGTCTTC ACTCACTGAC 
TGCTACGGAC CCTTCTCAGG ACAGCTCATC GCCACCATGA AGGAAGCCTG TCGCTATGCC 
CTGCAGGTGA AACCTCAGCG TCTGATGGCA GCAATGTACA CGTGTGACAT CATGGCCACC 
GGTGATGTTC TTGGTCGTGT CTATGCCGTC TTGTCAAAGA GAGAAGGTCG AGTGCTTCAA 
GAAGAAATGA AAGAAGGGAC AGACATGTTC ATCATCAAGG CGGTGCTGCC CGTTGCTGAG 
AGCTTCGGCT TTGCGGATGA GATCAGGAAG CGGACGAGTG GCTTGGCCAG CCCGCAGCTG 
GTGTTCAGCC ACTGGGAGAT CATTCCCAGC GACCCCTTCT GGGTGCCAAC CACCGAGGAA 
GAATACTTGC ACTTTGGGGA AAAGGCTGAT TCTGAGAACC AGGCCCGGAA GTACATGAAC 
GCCGTGCGCA AACGGAAGGG TCTTTACGTG GAAGAGAAGA TCGTGGAGCA CGCAGAAAAG 
CAGAGAACCC TCAGCAAAAA TAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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EFL1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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