Dpse\GA27495 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA27495
Entrez Gene ID	6897832
Full Name	GA27495 gene product from transcript GA27495-RA
Synonyms	GA27495,dpse_GLEANR_6255
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_002137901.2	XP_002137937.1	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA27495 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA27495 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe05831	XM_002137901.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA27495), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA27495 ( XM_002137901.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_002137937.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA27495), mRNA.

Price & Availability↑

CloneID	ODe05831
Clone ID Related Accession (Same CDS sequence)	XM_002137901.2
Accession Version	XM_002137901.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009005). On Dec 22, 2015 this sequence version replaced XM_002137901.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGTCGA TGAAAGCCAA CGAACTGCCG GAGAATCCCC GCGAGACCTC CAATCCCCTG 
TCGTCGCTGA TGTTCTGCTT TGCCATGCCC GTTCTCTTCA AGGGCCGCAA GAAGACCCTG 
GAGCAAAAGG ATCTCTATCA GGCCCTCAAG GAGCACAAGT CAGATTCATT GGGCGATCGA 
TTATGCGCTG CCTGGGACGA GGAGGTGGTC AGAAATAAGA ATCCCCGCCT GGGACGCGTT 
ATGACCAGAG TTTTTGGATG GCATTTGGTG CTCACTGGGG TGCTGCTGTT CCTACAGGAG 
TTCCTCACCA AGGTCACGCA GCCCATCTGT CTGTTTGGAA TAATGGCCTA CTTTGCCGGC 
GATGATACGG ATCTCTTCAA AGCGCAGCTC TATGCCACCG GCCTCATTGC TGCCTCCGTG 
TTCACAGTGT TCTTCGGCCA CCCTTACATG CTCGGCCTCC TTCATCTGGG GATGAAGATG 
CGCATTGCTC TGAGCAGCCT TATCTATCGG AAGTCCCTCC GACTCAGTCG CACTGCCCTG 
GGGGACACGA CGGTGGGGCA GGTGGTGAAT CTGCTGTCCA ACGATGTCGG ACGCTTTGAC 
ATGGTACTTA TCAACGTACA CTATCTCTGG GTGGCTCCAC TGGAGCTGAT AGTCGTCACG 
TATCTCATGT ACATCGAGAT TGGAGTATCT TCGCTGTTCG GCGTTGCCGT GATGCTCCTC 
TTCCTGCCTT TTCAGTCCTA TCTGGGAAAG CGGACGAGCG TCCTCCGACT GATGACTGCC 
CTGCGGACAG ACGAACGCGT GCGGATGATG AACGAGATAA TCTCGGGCAT TCAGGTGATC 
AAAATGTATG CCTGGGAGAA GCCTTTCGGA AAGCTGGTGG AGTTCACGCG CTTCAAGGAG 
ATGCAATGCA TCAAGCAGGT CAACTACATC CGCGGCATCC TCATCTCCTT CGCCATGTTC 
CTGTCTCGCA TCTTCATCTC CACCAGCCTC ATAGCCTTCG TTCTCCTGGG CAACGTCCTG 
ACCGCCGAGA AGGCCTTCTT TGTCACCGCC TACTACAACA TCTTGCGGCG GTCCGTGACG 
ATGTTCTTCC CGCAGGGAAT CTCGCAGTTT GCTGAGCTGC TCGTCTCCAT CCGCCGCCTG 
GAGACGTTCA TGCATCATCC GGAGACGCAA GTGCGCGACA AGTCAAAGAT CCAGAAGGAG 
GAGCCCGTGA TTGGTGATAG CCCAAAGGCC AACGGCCTGC CGGAGAAACT GCTGGACTTT 
AGCGGATTCA CAGCCAGGTG GGATAGCCAG AGCGCAGAGC CCACGCTGGA GAACATCAAT 
CTGCAGCTCG GACGCCGCAA GCTAGTGGCT GTGATAGGGC CCGTGGGCGC TGGGAAATCC 
AGCTTGATTC AGGCAGTGCT TGGGGAGCTG CCCGCGGAGA ATGGCAGCCT GCGGGTGGAC 
GGAAGTTACT CGTACGCCTC GCAGGAGCCG TGGCTCTTCA CGGGCACCGT GCGCCAGAAC 
ATCCTCTTCG GCCTGGAGTG GGACAAGCAT CGCTACCGCA CTGTCGTCAA GAAGTGCGCC 
CTGGAGCGGG ACTTTCAGCT GCTGCCGTAC GGCGACAAGA CGATTGTGGG CGAGAGAGGA 
GCCTCCCTCT CGGGCGGACA GAAGGCGCGC ATCAGCCTCG CTCGGGCAGT GTACCGCAGG 
GCAGACATCT ACCTCCTGGA CGATCCTCTC AGCGCAGTGG ACACGCACGT GGGGCGCCAC 
CTCTTCGATC AGTGCATGCA CGGGTACCTG CGCAGCGAAC TGGTGATCCT CGTCACGCAC 
CAGCTGCAGT TCCTGGAGCA AGCCGACCTC ATTGTTATCA TGGAAAAGGG CCGCATCAGC 
GCAATGGGCA CCTACTCTTC GATGAAGCGC AGTGGTCTGG ACTTTGCCAG GCTCTTGACC 
AATCCCAATA ACGAGGACGA TACAATGGAT GAGCTGGAGG TGGCCGTGGG GGACCAAATG 
GATCGCCTCA GTGTACCGTC GCTGAGCAGA CGTGGCAGCG GCAAAATCAG TCGCCCCACC 
AGCCGCAACA ATAGCTTCAC TTCCCTCAGC TCCATGGCCG AGTCGATGGC CCAGGAGGCA 
GCGCTGCAGA TGGAAGAGCC GCGGGTAGAG GGCAAGATCG GAGTGGGGCT CTACAAGGAG 
TATCTGACGG CAGGCAGCAG CTGGTTTATG ATCTCCTTCA TGTTGTTCCT CTGCCTGGCC 
ACCCAGATTG TTTGCTCTGC GGCGGATATC TTCCTCGCTT ATTGGGTCAA CAAGAACAGC 
AATAAGGCAG AGATGAGCTC GGATCCGGCG GACATGTACT ACTTCGCCGC CTTGAATGTG 
GCCGTCGTGG TCTTCACCCT CGTGCGGACG ATGCTCTTCT ACAAGATGGC GATGCGCAGC 
TCCACAACCC TCCACAACGC CATGTACCGC GGTATCACGC GAGCGGCCAT GTACTTCTTC 
AACACGAATC CCTCCGGCCG CATCCTCAAT CGCTTCTCCA AGGATCTGGG GCAGCTGGAT 
GAGCTGCTGC CCACCGTAAT GCTGGATGTG GTGCAGCTCT TCCTAATTCT CGCCGGCATC 
GTCGTTGTCA TTTGCATCAC GAATCCGTAC TACCTAATCC TGACGCTCAC TCTGGCGATC 
ATTTTCTACT ACATCCGAGA GTTCTACCTG AAGACATCGC GCGATGTGAA GCGGCTGGAG 
GCGGTGGCCA GGTCTCCGAT ATATTCGCAC CTGGGGGCCA CCATCAGTGG ATTGCCCACC 
ATCCGCGCTT TGGGAGCACA GAAGGCGCTC ATTGAAGAGT TCGACAATCT GCAGGATCTG 
CATAGTTCCG GCTACTACGC TTTCCTGGCG ACGAACCGAG CCTTTGGCTA CTACTTGGAC 
TTGTTCTGCA CGCTCTACAT CGTCATCATA ATTCTGAACT ACTTCATCAA TCCGCCGGAG 
AGCTCTGGCG AGGTCGGTCT GGCCATCACC CAGGCCATGG GCATGACCGG AATGGTGCAG 
TGGGCCATGC GGCAATCCGC GGAGCTGGAG AACACAATGA CCGCCGTGGA GCGGGTGTTG 
GAGTACGACG AGATCGAGCC GGAGGGAGAG TTCGAGTCGG ATCCGAAGAA GAAGCCCTGC 
GATACGTGGC CCGAGGAGGG AGAGATTATT GCCGAGGATC TCAGTCTGCG CTACTTCCCC 
GATCCGCAGT CCAAGTACGT GCTTCGGGCC CTGAACTTCC GCATCCGACC ATCGGAGAAG 
GTGGGCATTG TGGGACGCAC AGGAGCGGGC AAATCCTCGC TAATTAACGC CTTATTCCGA 
CTGTCCTACA ACGAAGGAAC CATCCACATC GACCACCGCG ACACGGCCGA CATCGGGCTG 
CACGATCTGC GCAGCAAGCT CTCAATCATT CCCCAGGAAC CGGTGCTCTT CTCGGGCAGT 
ATGCGGTACA ACCTCGATCC CTTCGAGGAG TACAGCGACG CCAAGCTCTG GGATGCCCTG 
GAGGAGGTGG AGCTGAAGCC CCTGATCAGC GAGCTGCCCA GCGGCCTGCA GAGCAAGATA 
TCCGAGGGCG GCCACAACTT CAGTGTGGGC CAGCGGCAGC TCGTATGCCT CGCCCGTGCC 
ATTCTCCGCG AGAACCGCAT CCTAGTGATG GACGAGGCTA CGGCCAATGT GGATCCGCAG 
ACAGATGCCC TCATCCAGGC CACCATCCGC AACAAATTCC GCGATTGCAC GGTGCTAACG 
ATTGCTCATC GCCTGAACAC AATCATGGAC TCGGATCGGG TCCTGGTGAT GGATGCCGGA 
CAGGTGGTGG AGTTTGGGTC ACCATACGAG CTCTTGACCG GCAGTGCCTC AAAGATATTC 
CATGGCATGG TCATGGAAGG TGGCCAGAGC CACTTCGATG GTCTCCTAAA GGTCGCTGAA 
AGGGCCCATC AGGAGTCTCA GAAACCCAAG TCGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002137937.1
CDS	950..4888
Translation	MQSMKANELPENPRETSNPLSSLMFCFAMPVLFKGRKKTLEQKDLYQALKEHKSDSLGDRLCAAWDEEVVRNKNPRLGRVMTRVFGWHLVLTGVLLFLQEFLTKVTQPICLFGIMAYFAGDDTDLFKAQLYATGLIAASVFTVFFGHPYMLGLLHLGMKMRIALSSLIYRKSLRLSRTALGDTTVGQVVNLLSNDVGRFDMVLINVHYLWVAPLELIVVTYLMYIEIGVSSLFGVAVMLLFLPFQSYLGKRTSVLRLMTALRTDERVRMMNEIISGIQVIKMYAWEKPFGKLVEFTRFKEMQCIKQVNYIRGILISFAMFLSRIFISTSLIAFVLLGNVLTAEKAFFVTAYYNILRRSVTMFFPQGISQFAELLVSIRRLETFMHHPETQVRDKSKIQKEEPVIGDSPKANGLPEKLLDFSGFTARWDSQSAEPTLENINLQLGRRKLVAVIGPVGAGKSSLIQAVLGELPAENGSLRVDGSYSYASQEPWLFTGTVRQNILFGLEWDKHRYRTVVKKCALERDFQLLPYGDKTIVGERGASLSGGQKARISLARAVYRRADIYLLDDPLSAVDTHVGRHLFDQCMHGYLRSELVILVTHQLQFLEQADLIVIMEKGRISAMGTYSSMKRSGLDFARLLTNPNNEDDTMDELEVAVGDQMDRLSVPSLSRRGSGKISRPTSRNNSFTSLSSMAESMAQEAALQMEEPRVEGKIGVGLYKEYLTAGSSWFMISFMLFLCLATQIVCSAADIFLAYWVNKNSNKAEMSSDPADMYYFAALNVAVVVFTLVRTMLFYKMAMRSSTTLHNAMYRGITRAAMYFFNTNPSGRILNRFSKDLGQLDELLPTVMLDVVQLFLILAGIVVVICITNPYYLILTLTLAIIFYYIREFYLKTSRDVKRLEAVARSPIYSHLGATISGLPTIRALGAQKALIEEFDNLQDLHSSGYYAFLATNRAFGYYLDLFCTLYIVIIILNYFINPPESSGEVGLAITQAMGMTGMVQWAMRQSAELENTMTAVERVLEYDEIEPEGEFESDPKKKPCDTWPEEGEIIAEDLSLRYFPDPQSKYVLRALNFRIRPSEKVGIVGRTGAGKSSLINALFRLSYNEGTIHIDHRDTADIGLHDLRSKLSIIPQEPVLFSGSMRYNLDPFEEYSDAKLWDALEEVELKPLISELPSGLQSKISEGGHNFSVGQRQLVCLARAILRENRILVMDEATANVDPQTDALIQATIRNKFRDCTVLTIAHRLNTIMDSDRVLVMDAGQVVEFGSPYELLTGSASKIFHGMVMEGGQSHFDGLLKVAERAHQESQKPKSE

Target ORF information:

RefSeq Version	XM_002137901.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA27495), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002137901.2

ORF Insert Sequence:

ATGCAGTCGA TGAAAGCCAA CGAACTGCCG GAGAATCCCC GCGAGACCTC CAATCCCCTG 
TCGTCGCTGA TGTTCTGCTT TGCCATGCCC GTTCTCTTCA AGGGCCGCAA GAAGACCCTG 
GAGCAAAAGG ATCTCTATCA GGCCCTCAAG GAGCACAAGT CAGATTCATT GGGCGATCGA 
TTATGCGCTG CCTGGGACGA GGAGGTGGTC AGAAATAAGA ATCCCCGCCT GGGACGCGTT 
ATGACCAGAG TTTTTGGATG GCATTTGGTG CTCACTGGGG TGCTGCTGTT CCTACAGGAG 
TTCCTCACCA AGGTCACGCA GCCCATCTGT CTGTTTGGAA TAATGGCCTA CTTTGCCGGC 
GATGATACGG ATCTCTTCAA AGCGCAGCTC TATGCCACCG GCCTCATTGC TGCCTCCGTG 
TTCACAGTGT TCTTCGGCCA CCCTTACATG CTCGGCCTCC TTCATCTGGG GATGAAGATG 
CGCATTGCTC TGAGCAGCCT TATCTATCGG AAGTCCCTCC GACTCAGTCG CACTGCCCTG 
GGGGACACGA CGGTGGGGCA GGTGGTGAAT CTGCTGTCCA ACGATGTCGG ACGCTTTGAC 
ATGGTACTTA TCAACGTACA CTATCTCTGG GTGGCTCCAC TGGAGCTGAT AGTCGTCACG 
TATCTCATGT ACATCGAGAT TGGAGTATCT TCGCTGTTCG GCGTTGCCGT GATGCTCCTC 
TTCCTGCCTT TTCAGTCCTA TCTGGGAAAG CGGACGAGCG TCCTCCGACT GATGACTGCC 
CTGCGGACAG ACGAACGCGT GCGGATGATG AACGAGATAA TCTCGGGCAT TCAGGTGATC 
AAAATGTATG CCTGGGAGAA GCCTTTCGGA AAGCTGGTGG AGTTCACGCG CTTCAAGGAG 
ATGCAATGCA TCAAGCAGGT CAACTACATC CGCGGCATCC TCATCTCCTT CGCCATGTTC 
CTGTCTCGCA TCTTCATCTC CACCAGCCTC ATAGCCTTCG TTCTCCTGGG CAACGTCCTG 
ACCGCCGAGA AGGCCTTCTT TGTCACCGCC TACTACAACA TCTTGCGGCG GTCCGTGACG 
ATGTTCTTCC CGCAGGGAAT CTCGCAGTTT GCTGAGCTGC TCGTCTCCAT CCGCCGCCTG 
GAGACGTTCA TGCATCATCC GGAGACGCAA GTGCGCGACA AGTCAAAGAT CCAGAAGGAG 
GAGCCCGTGA TTGGTGATAG CCCAAAGGCC AACGGCCTGC CGGAGAAACT GCTGGACTTT 
AGCGGATTCA CAGCCAGGTG GGATAGCCAG AGCGCAGAGC CCACGCTGGA GAACATCAAT 
CTGCAGCTCG GACGCCGCAA GCTAGTGGCT GTGATAGGGC CCGTGGGCGC TGGGAAATCC 
AGCTTGATTC AGGCAGTGCT TGGGGAGCTG CCCGCGGAGA ATGGCAGCCT GCGGGTGGAC 
GGAAGTTACT CGTACGCCTC GCAGGAGCCG TGGCTCTTCA CGGGCACCGT GCGCCAGAAC 
ATCCTCTTCG GCCTGGAGTG GGACAAGCAT CGCTACCGCA CTGTCGTCAA GAAGTGCGCC 
CTGGAGCGGG ACTTTCAGCT GCTGCCGTAC GGCGACAAGA CGATTGTGGG CGAGAGAGGA 
GCCTCCCTCT CGGGCGGACA GAAGGCGCGC ATCAGCCTCG CTCGGGCAGT GTACCGCAGG 
GCAGACATCT ACCTCCTGGA CGATCCTCTC AGCGCAGTGG ACACGCACGT GGGGCGCCAC 
CTCTTCGATC AGTGCATGCA CGGGTACCTG CGCAGCGAAC TGGTGATCCT CGTCACGCAC 
CAGCTGCAGT TCCTGGAGCA AGCCGACCTC ATTGTTATCA TGGAAAAGGG CCGCATCAGC 
GCAATGGGCA CCTACTCTTC GATGAAGCGC AGTGGTCTGG ACTTTGCCAG GCTCTTGACC 
AATCCCAATA ACGAGGACGA TACAATGGAT GAGCTGGAGG TGGCCGTGGG GGACCAAATG 
GATCGCCTCA GTGTACCGTC GCTGAGCAGA CGTGGCAGCG GCAAAATCAG TCGCCCCACC 
AGCCGCAACA ATAGCTTCAC TTCCCTCAGC TCCATGGCCG AGTCGATGGC CCAGGAGGCA 
GCGCTGCAGA TGGAAGAGCC GCGGGTAGAG GGCAAGATCG GAGTGGGGCT CTACAAGGAG 
TATCTGACGG CAGGCAGCAG CTGGTTTATG ATCTCCTTCA TGTTGTTCCT CTGCCTGGCC 
ACCCAGATTG TTTGCTCTGC GGCGGATATC TTCCTCGCTT ATTGGGTCAA CAAGAACAGC 
AATAAGGCAG AGATGAGCTC GGATCCGGCG GACATGTACT ACTTCGCCGC CTTGAATGTG 
GCCGTCGTGG TCTTCACCCT CGTGCGGACG ATGCTCTTCT ACAAGATGGC GATGCGCAGC 
TCCACAACCC TCCACAACGC CATGTACCGC GGTATCACGC GAGCGGCCAT GTACTTCTTC 
AACACGAATC CCTCCGGCCG CATCCTCAAT CGCTTCTCCA AGGATCTGGG GCAGCTGGAT 
GAGCTGCTGC CCACCGTAAT GCTGGATGTG GTGCAGCTCT TCCTAATTCT CGCCGGCATC 
GTCGTTGTCA TTTGCATCAC GAATCCGTAC TACCTAATCC TGACGCTCAC TCTGGCGATC 
ATTTTCTACT ACATCCGAGA GTTCTACCTG AAGACATCGC GCGATGTGAA GCGGCTGGAG 
GCGGTGGCCA GGTCTCCGAT ATATTCGCAC CTGGGGGCCA CCATCAGTGG ATTGCCCACC 
ATCCGCGCTT TGGGAGCACA GAAGGCGCTC ATTGAAGAGT TCGACAATCT GCAGGATCTG 
CATAGTTCCG GCTACTACGC TTTCCTGGCG ACGAACCGAG CCTTTGGCTA CTACTTGGAC 
TTGTTCTGCA CGCTCTACAT CGTCATCATA ATTCTGAACT ACTTCATCAA TCCGCCGGAG 
AGCTCTGGCG AGGTCGGTCT GGCCATCACC CAGGCCATGG GCATGACCGG AATGGTGCAG 
TGGGCCATGC GGCAATCCGC GGAGCTGGAG AACACAATGA CCGCCGTGGA GCGGGTGTTG 
GAGTACGACG AGATCGAGCC GGAGGGAGAG TTCGAGTCGG ATCCGAAGAA GAAGCCCTGC 
GATACGTGGC CCGAGGAGGG AGAGATTATT GCCGAGGATC TCAGTCTGCG CTACTTCCCC 
GATCCGCAGT CCAAGTACGT GCTTCGGGCC CTGAACTTCC GCATCCGACC ATCGGAGAAG 
GTGGGCATTG TGGGACGCAC AGGAGCGGGC AAATCCTCGC TAATTAACGC CTTATTCCGA 
CTGTCCTACA ACGAAGGAAC CATCCACATC GACCACCGCG ACACGGCCGA CATCGGGCTG 
CACGATCTGC GCAGCAAGCT CTCAATCATT CCCCAGGAAC CGGTGCTCTT CTCGGGCAGT 
ATGCGGTACA ACCTCGATCC CTTCGAGGAG TACAGCGACG CCAAGCTCTG GGATGCCCTG 
GAGGAGGTGG AGCTGAAGCC CCTGATCAGC GAGCTGCCCA GCGGCCTGCA GAGCAAGATA 
TCCGAGGGCG GCCACAACTT CAGTGTGGGC CAGCGGCAGC TCGTATGCCT CGCCCGTGCC 
ATTCTCCGCG AGAACCGCAT CCTAGTGATG GACGAGGCTA CGGCCAATGT GGATCCGCAG 
ACAGATGCCC TCATCCAGGC CACCATCCGC AACAAATTCC GCGATTGCAC GGTGCTAACG 
ATTGCTCATC GCCTGAACAC AATCATGGAC TCGGATCGGG TCCTGGTGAT GGATGCCGGA 
CAGGTGGTGG AGTTTGGGTC ACCATACGAG CTCTTGACCG GCAGTGCCTC AAAGATATTC 
CATGGCATGG TCATGGAAGG TGGCCAGAGC CACTTCGATG GTCTCCTAAA GGTCGCTGAA 
AGGGCCCATC AGGAGTCTCA GAAACCCAAG TCGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA27495 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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