Prosbeta5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prosbeta5
Entrez Gene ID	45269
Full Name	Proteasome beta5 subunit
Synonyms	Beta-5,CG12323,Dmel\CG12323,LD08717,PSMB5,Pros b5,anon-WO0118547.116,beta-5,beta5,dbeta5
General protein information	Preferred Names Proteasome beta5 subunit Names CG12323 gene product from transcript CG12323-RA 20S proteasome CG12323-PA CG12323-PB Prosbeta5-PA Prosbeta5-PB beta5 proteasome beta5 subunit
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 47C1-47C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_143757.4	NP_652014.1	proteasome beta5 subunit, isoform A
NM_165801.2	NP_724974.1	proteasome beta5 subunit, isoform B

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PSMB5	NP_001032701.1
Drosophila melanogaster (fruit fly)	Prosbeta5	NP_724974.1
Arabidopsis thaliana (thale cress)	PBE1	NP_172765.1
Canis lupus familiaris (dog)	PSMB5	XP_003639276.1
Macaca mulatta (Rhesus monkey)	PSMB5	XP_001105158.1
Mus musculus (house mouse)	Psmb5	NP_035316.1
Danio rerio (zebrafish)	psmb5	NP_571226.1
Xenopus tropicalis (tropical clawed frog)	psmb5	XP_002941560.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010718	XP_559226.2
Homo sapiens (human)	PSMB5	NP_002788.1
Rattus norvegicus (Norway rat)	Psmb5	NP_001099197.2
Arabidopsis thaliana (thale cress)	AT3G26340	NP_189265.1
Oryza sativa (rice)	Os06g0153800	NP_001056842.1
Caenorhabditis elegans (roundworm)	pbs-5	NP_493558.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Prosbeta5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prosbeta5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11388	NM_165801.2 Latest version!	Drosophila melanogaster proteasome beta5 subunit, transcript variant B (Prosbeta5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OFa11388	NM_143757.4 Latest version!	Drosophila melanogaster proteasome beta5 subunit, transcript variant A (Prosbeta5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prosbeta5 ( NM_143757.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_652014.1 Drosophila melanogaster proteasome beta5 subunit, transcript variant A (Prosbeta5), mRNA.

Price & Availability↑

CloneID	OFa11388
Clone ID Related Accession (Same CDS sequence)	NM_143757.4 , NM_165801.2
Accession Version	NM_143757.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	proteasome beta5 subunit, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_143757.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTTTAG CTGAAATCTG CAAGATATCG AATGCTCCGT ACATGCGGCC CAATGCCTGG 
TCATCGGCGG ATGTGGAGGA AGAGCAAAAA GGGCTTATGT GCAATCTGGC CAATCCCTAC 
ACACTGGCTG CTCCGCCATT CGAGAACCCC CTGCACAACC TTAATCAGAT CCAGGCCAAT 
GGCGACAAGA CCGGCGTCAA AATCAACTTC GATCACGGCA CCACAACGTT GGGCTTCAAG 
TTCAAGGGCG GCGTTCTCCT GGCAGTCGAT TCCCGTGCCA CGGGTGGATC GTACATTGGC 
TCCCAGTCGA TGAAGAAGAT CGTGGAGATC AATCAGTTCA TGCTGGGCAC CTTGGCCGGT 
GGCGCAGCCG ATTGCGTTTA CTGGGACAGG GTCCTTTCGA AGGAATGCCG CCTTCACGAG 
CTTCGAAACA AGGAACGCAT CTCGGTGGCC GCCGCCAGCA AGATAATGGC CAACATTGCC 
CACGAATACA AGGGAATGGG TCTGAGCATG GGCATGATGC TGGCCGGTTA CGATAAGCGT 
GGTCCAGGCC TCTACTATGT GGACTCCGAG GGATCTCGCA CGCCTGGCAA TTTGTTCTCT 
GTTGGTAGTG GATCGCTGTA CGCCTACGGT GTCCTGGACT CTGGCTATCA TTGGGACCTG 
GAGGACAAGG AGGCCCAGGA GCTGGGACGT CGCGCCATCT ACCATGCCAC CTTCAGGGAT 
GCCTACTCCG GTGGTATCAT TCGCGTGTAT CACATTAAGG AGGACGGCTG GGTAAACATC 
TCCAACACCG ACTGCATGGA GCTGCACTAC ATGTACCAGG AGCAGTTGAA GCAGCAGGCC 
GCTAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_652014.1
CDS	96..944
Translation	MALAEICKISNAPYMRPNAWSSADVEEEQKGLMCNLANPYTLAAPPFENPLHNLNQIQANGDKTGVKINFDHGTTTLGFKFKGGVLLAVDSRATGGSYIGSQSMKKIVEINQFMLGTLAGGAADCVYWDRVLSKECRLHELRNKERISVAAASKIMANIAHEYKGMGLSMGMMLAGYDKRGPGLYYVDSEGSRTPGNLFSVGSGSLYAYGVLDSGYHWDLEDKEAQELGRRAIYHATFRDAYSGGIIRVYHIKEDGWVNISNTDCMELHYMYQEQLKQQAAK

Target ORF information:

RefSeq Version	NM_143757.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster proteasome beta5 subunit, transcript variant A (Prosbeta5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143757.4

ORF Insert Sequence:

ATGGCTTTAG CTGAAATCTG CAAGATATCG AATGCTCCGT ACATGCGGCC CAATGCCTGG 
TCATCGGCGG ATGTGGAGGA AGAGCAAAAA GGGCTTATGT GCAATCTGGC CAATCCCTAC 
ACACTGGCTG CTCCGCCATT CGAGAACCCC CTGCACAACC TTAATCAGAT CCAGGCCAAT 
GGCGACAAGA CCGGCGTCAA AATCAACTTC GATCACGGCA CCACAACGTT GGGCTTCAAG 
TTCAAGGGCG GCGTTCTCCT GGCAGTCGAT TCCCGTGCCA CGGGTGGATC GTACATTGGC 
TCCCAGTCGA TGAAGAAGAT CGTGGAGATC AATCAGTTCA TGCTGGGCAC CTTGGCCGGT 
GGCGCAGCCG ATTGCGTTTA CTGGGACAGG GTCCTTTCGA AGGAATGCCG CCTTCACGAG 
CTTCGAAACA AGGAACGCAT CTCGGTGGCC GCCGCCAGCA AGATAATGGC CAACATTGCC 
CACGAATACA AGGGAATGGG TCTGAGCATG GGCATGATGC TGGCCGGTTA CGATAAGCGT 
GGTCCAGGCC TCTACTATGT GGACTCCGAG GGATCTCGCA CGCCTGGCAA TTTGTTCTCT 
GTTGGTAGTG GATCGCTGTA CGCCTACGGT GTCCTGGACT CTGGCTATCA TTGGGACCTG 
GAGGACAAGG AGGCCCAGGA GCTGGGACGT CGCGCCATCT ACCATGCCAC CTTCAGGGAT 
GCCTACTCCG GTGGTATCAT TCGCGTGTAT CACATTAAGG AGGACGGCTG GGTAAACATC 
TCCAACACCG ACTGCATGGA GCTGCACTAC ATGTACCAGG AGCAGTTGAA GCAGCAGGCC 
GCTAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prosbeta5 ( NM_165801.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724974.1 Drosophila melanogaster proteasome beta5 subunit, transcript variant B (Prosbeta5), mRNA.

Price & Availability↑

CloneID	OFa11388
Clone ID Related Accession (Same CDS sequence)	NM_143757.4 , NM_165801.2
Accession Version	NM_165801.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	proteasome beta5 subunit, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_165801.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTTTAG CTGAAATCTG CAAGATATCG AATGCTCCGT ACATGCGGCC CAATGCCTGG 
TCATCGGCGG ATGTGGAGGA AGAGCAAAAA GGGCTTATGT GCAATCTGGC CAATCCCTAC 
ACACTGGCTG CTCCGCCATT CGAGAACCCC CTGCACAACC TTAATCAGAT CCAGGCCAAT 
GGCGACAAGA CCGGCGTCAA AATCAACTTC GATCACGGCA CCACAACGTT GGGCTTCAAG 
TTCAAGGGCG GCGTTCTCCT GGCAGTCGAT TCCCGTGCCA CGGGTGGATC GTACATTGGC 
TCCCAGTCGA TGAAGAAGAT CGTGGAGATC AATCAGTTCA TGCTGGGCAC CTTGGCCGGT 
GGCGCAGCCG ATTGCGTTTA CTGGGACAGG GTCCTTTCGA AGGAATGCCG CCTTCACGAG 
CTTCGAAACA AGGAACGCAT CTCGGTGGCC GCCGCCAGCA AGATAATGGC CAACATTGCC 
CACGAATACA AGGGAATGGG TCTGAGCATG GGCATGATGC TGGCCGGTTA CGATAAGCGT 
GGTCCAGGCC TCTACTATGT GGACTCCGAG GGATCTCGCA CGCCTGGCAA TTTGTTCTCT 
GTTGGTAGTG GATCGCTGTA CGCCTACGGT GTCCTGGACT CTGGCTATCA TTGGGACCTG 
GAGGACAAGG AGGCCCAGGA GCTGGGACGT CGCGCCATCT ACCATGCCAC CTTCAGGGAT 
GCCTACTCCG GTGGTATCAT TCGCGTGTAT CACATTAAGG AGGACGGCTG GGTAAACATC 
TCCAACACCG ACTGCATGGA GCTGCACTAC ATGTACCAGG AGCAGTTGAA GCAGCAGGCC 
GCTAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724974.1
CDS	33..881
Translation	MALAEICKISNAPYMRPNAWSSADVEEEQKGLMCNLANPYTLAAPPFENPLHNLNQIQANGDKTGVKINFDHGTTTLGFKFKGGVLLAVDSRATGGSYIGSQSMKKIVEINQFMLGTLAGGAADCVYWDRVLSKECRLHELRNKERISVAAASKIMANIAHEYKGMGLSMGMMLAGYDKRGPGLYYVDSEGSRTPGNLFSVGSGSLYAYGVLDSGYHWDLEDKEAQELGRRAIYHATFRDAYSGGIIRVYHIKEDGWVNISNTDCMELHYMYQEQLKQQAAK

Target ORF information:

RefSeq Version	NM_165801.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster proteasome beta5 subunit, transcript variant B (Prosbeta5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165801.2

ORF Insert Sequence:

ATGGCTTTAG CTGAAATCTG CAAGATATCG AATGCTCCGT ACATGCGGCC CAATGCCTGG 
TCATCGGCGG ATGTGGAGGA AGAGCAAAAA GGGCTTATGT GCAATCTGGC CAATCCCTAC 
ACACTGGCTG CTCCGCCATT CGAGAACCCC CTGCACAACC TTAATCAGAT CCAGGCCAAT 
GGCGACAAGA CCGGCGTCAA AATCAACTTC GATCACGGCA CCACAACGTT GGGCTTCAAG 
TTCAAGGGCG GCGTTCTCCT GGCAGTCGAT TCCCGTGCCA CGGGTGGATC GTACATTGGC 
TCCCAGTCGA TGAAGAAGAT CGTGGAGATC AATCAGTTCA TGCTGGGCAC CTTGGCCGGT 
GGCGCAGCCG ATTGCGTTTA CTGGGACAGG GTCCTTTCGA AGGAATGCCG CCTTCACGAG 
CTTCGAAACA AGGAACGCAT CTCGGTGGCC GCCGCCAGCA AGATAATGGC CAACATTGCC 
CACGAATACA AGGGAATGGG TCTGAGCATG GGCATGATGC TGGCCGGTTA CGATAAGCGT 
GGTCCAGGCC TCTACTATGT GGACTCCGAG GGATCTCGCA CGCCTGGCAA TTTGTTCTCT 
GTTGGTAGTG GATCGCTGTA CGCCTACGGT GTCCTGGACT CTGGCTATCA TTGGGACCTG 
GAGGACAAGG AGGCCCAGGA GCTGGGACGT CGCGCCATCT ACCATGCCAC CTTCAGGGAT 
GCCTACTCCG GTGGTATCAT TCGCGTGTAT CACATTAAGG AGGACGGCTG GGTAAACATC 
TCCAACACCG ACTGCATGGA GCTGCACTAC ATGTACCAGG AGCAGTTGAA GCAGCAGGCC 
GCTAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Prosbeta5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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