RpS8 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	RpS8
Entrez Gene ID	43532
Full Name	Ribosomal protein S8
Synonyms	BcDNA:RH06886,BcDNA:SD17528,CG7808,Dmel\CG7808,Rp S8,Rps8,S8,anon-EST:Liang-1.56,anon-EST:fe1A2,chr3R:25688477..25688608,clone 1.56
General protein information	Preferred Names Ribosomal protein S8 Names CG7808 gene product from transcript CG7808-RG CG7808-PC CG7808-PE CG7808-PF CG7808-PG RpS8-PC RpS8-PE RpS8-PF RpS8-PG anon-fast-evolving-1A2 ribosomal protein S8
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 99C4-99C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001260433.3	NP_001247362.1	ribosomal protein S8, isoform E
NM_001300667.1	NP_001287596.1	ribosomal protein S8, isoform F
NM_143483.3	NP_651740.1	ribosomal protein S8, isoform C
NM_001300668.1	NP_001287597.1	ribosomal protein S8, isoform G

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	RpS8	NP_001247362.1
Caenorhabditis elegans (roundworm)	rps-8	NP_501167.1
Xenopus tropicalis (tropical clawed frog)	rps8	NP_001016566.1
Schizosaccharomyces pombe (fission yeast)	rps802	NP_593100.1
Homo sapiens (human)	RPS8	NP_001003.1
Macaca mulatta (Rhesus monkey)	LOC696015	XP_002804244.1
Canis lupus familiaris (dog)	RPS8	XP_862274.2
Rattus norvegicus (Norway rat)	Rps8	NP_113894.1
Kluyveromyces lactis	KLLA0E20461g	XP_454876.1
Eremothecium gossypii	AGOS_AGR375W	NP_987041.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002437	XP_312508.3
Bos taurus (cattle)	RPS8	NP_001020488.1
Arabidopsis thaliana (thale cress)	AT5G59240	NP_200732.2
Danio rerio (zebrafish)	rps8b	NP_001128155.1
Schizosaccharomyces pombe (fission yeast)	rps801	NP_594519.1
Mus musculus (house mouse)	Rps8	NP_033124.1
Gallus gallus (chicken)	RPS8	NP_001239055.1
Saccharomyces cerevisiae (baker's yeast)	RPS8A	NP_009481.1
Magnaporthe oryzae (rice blast fungus)	MGG_03251	XP_003716723.1
Neurospora crassa	NCU08500	XP_963429.1
Saccharomyces cerevisiae (baker's yeast)	RPS8B	NP_011028.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RpS8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RpS8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06557	NM_001300668.1 Latest version!	Drosophila melanogaster ribosomal protein S8, transcript variant G (RpS8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OFa06557	NM_001260433.3 Latest version!	Drosophila melanogaster ribosomal protein S8, transcript variant E (RpS8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OFa06557	NM_143483.3 Latest version!	Drosophila melanogaster ribosomal protein S8, transcript variant C (RpS8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OFa06557	NM_001300667.1 Latest version!	Drosophila melanogaster ribosomal protein S8, transcript variant F (RpS8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RpS8 ( NM_001260433.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247362.1 Drosophila melanogaster ribosomal protein S8, transcript variant E (RpS8), mRNA.

Price & Availability↑

CloneID	OFa06557
Clone ID Related Accession (Same CDS sequence)	NM_001260433.3 , NM_001300667.1 , NM_143483.3 , NM_001300668.1
Accession Version	NM_001260433.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	ribosomal protein S8, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001260433.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTATTA GCCGCGATAG TGCACACAAA CGCCGGGCCA CCGGAGGCAA GCGCAAGTCG 
CTCCGCAAGA AGCGCAAGTT CGAGTTGGGA CGCCCCGCCG CCAACACCAA GCTTGGCTCC 
GGCCGCGTGC ACAAGGTGCG CACCCGTGGT GGAAACACCA AGCTCCGTGC TCTGCGCCTG 
GAAACCGGAA ACTTCGCCTG GGCCTCCGAG GGAGTGGCGC GCAAGACCCG TATCGCCGAT 
GTTGTGTACA ACGCCTCCAA CAACGAGCTG GTGCGAACCA AGACCTTGGT GAAGAACAGC 
ATCGTGGTCA TCGATGCCAC GCCCTTCCGC CAGTGGTACG AGGCTCACTA CGTGCTGCCC 
CTGGGACGCA AGCGTAACCC CAAGCACGCC CAGAAAGAGG ACGAGAACGA CGTGCTGACC 
AAGAAGCGCA GCGAAAAGGT TATGAAGAAG TACCTGGAGC GCCAGAAGTA CGGCAAGGTC 
GAGCAGGCCC TCGAGGATCA GTTCACCTCC GGCCGCATCT TGGCTTGCAT TTCTTCCCGC 
CCCGGACAGT GCGGTCGCTC CGACGGCTAC ATTTTGGAAG GCAAGGAGTT GGAATTCTAC 
CTTAAGAAGA TCAAGTCTAA GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247362.1
CDS	65..691
Translation	MGISRDSAHKRRATGGKRKSLRKKRKFELGRPAANTKLGSGRVHKVRTRGGNTKLRALRLETGNFAWASEGVARKTRIADVVYNASNNELVRTKTLVKNSIVVIDATPFRQWYEAHYVLPLGRKRNPKHAQKEDENDVLTKKRSEKVMKKYLERQKYGKVEQALEDQFTSGRILACISSRPGQCGRSDGYILEGKELEFYLKKIKSKK

Target ORF information:

RefSeq Version	NM_001260433.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ribosomal protein S8, transcript variant E (RpS8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260433.3

ORF Insert Sequence:

ATGGGTATTA GCCGCGATAG TGCACACAAA CGCCGGGCCA CCGGAGGCAA GCGCAAGTCG 
CTCCGCAAGA AGCGCAAGTT CGAGTTGGGA CGCCCCGCCG CCAACACCAA GCTTGGCTCC 
GGCCGCGTGC ACAAGGTGCG CACCCGTGGT GGAAACACCA AGCTCCGTGC TCTGCGCCTG 
GAAACCGGAA ACTTCGCCTG GGCCTCCGAG GGAGTGGCGC GCAAGACCCG TATCGCCGAT 
GTTGTGTACA ACGCCTCCAA CAACGAGCTG GTGCGAACCA AGACCTTGGT GAAGAACAGC 
ATCGTGGTCA TCGATGCCAC GCCCTTCCGC CAGTGGTACG AGGCTCACTA CGTGCTGCCC 
CTGGGACGCA AGCGTAACCC CAAGCACGCC CAGAAAGAGG ACGAGAACGA CGTGCTGACC 
AAGAAGCGCA GCGAAAAGGT TATGAAGAAG TACCTGGAGC GCCAGAAGTA CGGCAAGGTC 
GAGCAGGCCC TCGAGGATCA GTTCACCTCC GGCCGCATCT TGGCTTGCAT TTCTTCCCGC 
CCCGGACAGT GCGGTCGCTC CGACGGCTAC ATTTTGGAAG GCAAGGAGTT GGAATTCTAC 
CTTAAGAAGA TCAAGTCTAA GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RpS8 ( NM_001300667.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287596.1 Drosophila melanogaster ribosomal protein S8, transcript variant F (RpS8), mRNA.

Price & Availability↑

CloneID	OFa06557
Clone ID Related Accession (Same CDS sequence)	NM_001260433.3 , NM_001300667.1 , NM_143483.3 , NM_001300668.1
Accession Version	NM_001300667.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	ribosomal protein S8, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTATTA GCCGCGATAG TGCACACAAA CGCCGGGCCA CCGGAGGCAA GCGCAAGTCG 
CTCCGCAAGA AGCGCAAGTT CGAGTTGGGA CGCCCCGCCG CCAACACCAA GCTTGGCTCC 
GGCCGCGTGC ACAAGGTGCG CACCCGTGGT GGAAACACCA AGCTCCGTGC TCTGCGCCTG 
GAAACCGGAA ACTTCGCCTG GGCCTCCGAG GGAGTGGCGC GCAAGACCCG TATCGCCGAT 
GTTGTGTACA ACGCCTCCAA CAACGAGCTG GTGCGAACCA AGACCTTGGT GAAGAACAGC 
ATCGTGGTCA TCGATGCCAC GCCCTTCCGC CAGTGGTACG AGGCTCACTA CGTGCTGCCC 
CTGGGACGCA AGCGTAACCC CAAGCACGCC CAGAAAGAGG ACGAGAACGA CGTGCTGACC 
AAGAAGCGCA GCGAAAAGGT TATGAAGAAG TACCTGGAGC GCCAGAAGTA CGGCAAGGTC 
GAGCAGGCCC TCGAGGATCA GTTCACCTCC GGCCGCATCT TGGCTTGCAT TTCTTCCCGC 
CCCGGACAGT GCGGTCGCTC CGACGGCTAC ATTTTGGAAG GCAAGGAGTT GGAATTCTAC 
CTTAAGAAGA TCAAGTCTAA GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287596.1
CDS	35..661
Translation	MGISRDSAHKRRATGGKRKSLRKKRKFELGRPAANTKLGSGRVHKVRTRGGNTKLRALRLETGNFAWASEGVARKTRIADVVYNASNNELVRTKTLVKNSIVVIDATPFRQWYEAHYVLPLGRKRNPKHAQKEDENDVLTKKRSEKVMKKYLERQKYGKVEQALEDQFTSGRILACISSRPGQCGRSDGYILEGKELEFYLKKIKSKK

Target ORF information:

RefSeq Version	NM_001300667.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ribosomal protein S8, transcript variant F (RpS8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300667.1

ORF Insert Sequence:

ATGGGTATTA GCCGCGATAG TGCACACAAA CGCCGGGCCA CCGGAGGCAA GCGCAAGTCG 
CTCCGCAAGA AGCGCAAGTT CGAGTTGGGA CGCCCCGCCG CCAACACCAA GCTTGGCTCC 
GGCCGCGTGC ACAAGGTGCG CACCCGTGGT GGAAACACCA AGCTCCGTGC TCTGCGCCTG 
GAAACCGGAA ACTTCGCCTG GGCCTCCGAG GGAGTGGCGC GCAAGACCCG TATCGCCGAT 
GTTGTGTACA ACGCCTCCAA CAACGAGCTG GTGCGAACCA AGACCTTGGT GAAGAACAGC 
ATCGTGGTCA TCGATGCCAC GCCCTTCCGC CAGTGGTACG AGGCTCACTA CGTGCTGCCC 
CTGGGACGCA AGCGTAACCC CAAGCACGCC CAGAAAGAGG ACGAGAACGA CGTGCTGACC 
AAGAAGCGCA GCGAAAAGGT TATGAAGAAG TACCTGGAGC GCCAGAAGTA CGGCAAGGTC 
GAGCAGGCCC TCGAGGATCA GTTCACCTCC GGCCGCATCT TGGCTTGCAT TTCTTCCCGC 
CCCGGACAGT GCGGTCGCTC CGACGGCTAC ATTTTGGAAG GCAAGGAGTT GGAATTCTAC 
CTTAAGAAGA TCAAGTCTAA GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RpS8 ( NM_143483.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651740.1 Drosophila melanogaster ribosomal protein S8, transcript variant C (RpS8), mRNA.

Price & Availability↑

CloneID	OFa06557
Clone ID Related Accession (Same CDS sequence)	NM_001260433.3 , NM_001300667.1 , NM_143483.3 , NM_001300668.1
Accession Version	NM_143483.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	ribosomal protein S8, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143483.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTATTA GCCGCGATAG TGCACACAAA CGCCGGGCCA CCGGAGGCAA GCGCAAGTCG 
CTCCGCAAGA AGCGCAAGTT CGAGTTGGGA CGCCCCGCCG CCAACACCAA GCTTGGCTCC 
GGCCGCGTGC ACAAGGTGCG CACCCGTGGT GGAAACACCA AGCTCCGTGC TCTGCGCCTG 
GAAACCGGAA ACTTCGCCTG GGCCTCCGAG GGAGTGGCGC GCAAGACCCG TATCGCCGAT 
GTTGTGTACA ACGCCTCCAA CAACGAGCTG GTGCGAACCA AGACCTTGGT GAAGAACAGC 
ATCGTGGTCA TCGATGCCAC GCCCTTCCGC CAGTGGTACG AGGCTCACTA CGTGCTGCCC 
CTGGGACGCA AGCGTAACCC CAAGCACGCC CAGAAAGAGG ACGAGAACGA CGTGCTGACC 
AAGAAGCGCA GCGAAAAGGT TATGAAGAAG TACCTGGAGC GCCAGAAGTA CGGCAAGGTC 
GAGCAGGCCC TCGAGGATCA GTTCACCTCC GGCCGCATCT TGGCTTGCAT TTCTTCCCGC 
CCCGGACAGT GCGGTCGCTC CGACGGCTAC ATTTTGGAAG GCAAGGAGTT GGAATTCTAC 
CTTAAGAAGA TCAAGTCTAA GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651740.1
CDS	35..661
Translation	MGISRDSAHKRRATGGKRKSLRKKRKFELGRPAANTKLGSGRVHKVRTRGGNTKLRALRLETGNFAWASEGVARKTRIADVVYNASNNELVRTKTLVKNSIVVIDATPFRQWYEAHYVLPLGRKRNPKHAQKEDENDVLTKKRSEKVMKKYLERQKYGKVEQALEDQFTSGRILACISSRPGQCGRSDGYILEGKELEFYLKKIKSKK

Target ORF information:

RefSeq Version	NM_143483.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ribosomal protein S8, transcript variant C (RpS8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143483.3

ORF Insert Sequence:

ATGGGTATTA GCCGCGATAG TGCACACAAA CGCCGGGCCA CCGGAGGCAA GCGCAAGTCG 
CTCCGCAAGA AGCGCAAGTT CGAGTTGGGA CGCCCCGCCG CCAACACCAA GCTTGGCTCC 
GGCCGCGTGC ACAAGGTGCG CACCCGTGGT GGAAACACCA AGCTCCGTGC TCTGCGCCTG 
GAAACCGGAA ACTTCGCCTG GGCCTCCGAG GGAGTGGCGC GCAAGACCCG TATCGCCGAT 
GTTGTGTACA ACGCCTCCAA CAACGAGCTG GTGCGAACCA AGACCTTGGT GAAGAACAGC 
ATCGTGGTCA TCGATGCCAC GCCCTTCCGC CAGTGGTACG AGGCTCACTA CGTGCTGCCC 
CTGGGACGCA AGCGTAACCC CAAGCACGCC CAGAAAGAGG ACGAGAACGA CGTGCTGACC 
AAGAAGCGCA GCGAAAAGGT TATGAAGAAG TACCTGGAGC GCCAGAAGTA CGGCAAGGTC 
GAGCAGGCCC TCGAGGATCA GTTCACCTCC GGCCGCATCT TGGCTTGCAT TTCTTCCCGC 
CCCGGACAGT GCGGTCGCTC CGACGGCTAC ATTTTGGAAG GCAAGGAGTT GGAATTCTAC 
CTTAAGAAGA TCAAGTCTAA GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RpS8 ( NM_001300668.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287597.1 Drosophila melanogaster ribosomal protein S8, transcript variant G (RpS8), mRNA.

Price & Availability↑

CloneID	OFa06557
Clone ID Related Accession (Same CDS sequence)	NM_001260433.3 , NM_001300667.1 , NM_143483.3 , NM_001300668.1
Accession Version	NM_001300668.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	ribosomal protein S8, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTATTA GCCGCGATAG TGCACACAAA CGCCGGGCCA CCGGAGGCAA GCGCAAGTCG 
CTCCGCAAGA AGCGCAAGTT CGAGTTGGGA CGCCCCGCCG CCAACACCAA GCTTGGCTCC 
GGCCGCGTGC ACAAGGTGCG CACCCGTGGT GGAAACACCA AGCTCCGTGC TCTGCGCCTG 
GAAACCGGAA ACTTCGCCTG GGCCTCCGAG GGAGTGGCGC GCAAGACCCG TATCGCCGAT 
GTTGTGTACA ACGCCTCCAA CAACGAGCTG GTGCGAACCA AGACCTTGGT GAAGAACAGC 
ATCGTGGTCA TCGATGCCAC GCCCTTCCGC CAGTGGTACG AGGCTCACTA CGTGCTGCCC 
CTGGGACGCA AGCGTAACCC CAAGCACGCC CAGAAAGAGG ACGAGAACGA CGTGCTGACC 
AAGAAGCGCA GCGAAAAGGT TATGAAGAAG TACCTGGAGC GCCAGAAGTA CGGCAAGGTC 
GAGCAGGCCC TCGAGGATCA GTTCACCTCC GGCCGCATCT TGGCTTGCAT TTCTTCCCGC 
CCCGGACAGT GCGGTCGCTC CGACGGCTAC ATTTTGGAAG GCAAGGAGTT GGAATTCTAC 
CTTAAGAAGA TCAAGTCTAA GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287597.1
CDS	240..866
Translation	MGISRDSAHKRRATGGKRKSLRKKRKFELGRPAANTKLGSGRVHKVRTRGGNTKLRALRLETGNFAWASEGVARKTRIADVVYNASNNELVRTKTLVKNSIVVIDATPFRQWYEAHYVLPLGRKRNPKHAQKEDENDVLTKKRSEKVMKKYLERQKYGKVEQALEDQFTSGRILACISSRPGQCGRSDGYILEGKELEFYLKKIKSKK

Target ORF information:

RefSeq Version	NM_001300668.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ribosomal protein S8, transcript variant G (RpS8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300668.1

ORF Insert Sequence:

ATGGGTATTA GCCGCGATAG TGCACACAAA CGCCGGGCCA CCGGAGGCAA GCGCAAGTCG 
CTCCGCAAGA AGCGCAAGTT CGAGTTGGGA CGCCCCGCCG CCAACACCAA GCTTGGCTCC 
GGCCGCGTGC ACAAGGTGCG CACCCGTGGT GGAAACACCA AGCTCCGTGC TCTGCGCCTG 
GAAACCGGAA ACTTCGCCTG GGCCTCCGAG GGAGTGGCGC GCAAGACCCG TATCGCCGAT 
GTTGTGTACA ACGCCTCCAA CAACGAGCTG GTGCGAACCA AGACCTTGGT GAAGAACAGC 
ATCGTGGTCA TCGATGCCAC GCCCTTCCGC CAGTGGTACG AGGCTCACTA CGTGCTGCCC 
CTGGGACGCA AGCGTAACCC CAAGCACGCC CAGAAAGAGG ACGAGAACGA CGTGCTGACC 
AAGAAGCGCA GCGAAAAGGT TATGAAGAAG TACCTGGAGC GCCAGAAGTA CGGCAAGGTC 
GAGCAGGCCC TCGAGGATCA GTTCACCTCC GGCCGCATCT TGGCTTGCAT TTCTTCCCGC 
CCCGGACAGT GCGGTCGCTC CGACGGCTAC ATTTTGGAAG GCAAGGAGTT GGAATTCTAC 
CTTAAGAAGA TCAAGTCTAA GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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RpS8 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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