Irk2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Irk2
Entrez Gene ID	42770
Full Name	Inwardly rectifying potassium channel 2
Synonyms	CG10180,CG4370,Dmel\CG4370,dKir,dKirII,irk2
General protein information	Preferred Names Inwardly rectifying potassium channel 2 Names CG4370 gene product from transcript CG4370-RD CG4370-PA CG4370-PB CG4370-PC CG4370-PD Irk2-PA Irk2-PB Irk2-PC Irk2-PD inwardly rectifying potassium channel 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 95A1-95A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001170229.2	NP_001163700.1	inwardly rectifying potassium channel 2, isoform C
NM_170076.2	NP_732871.1	inwardly rectifying potassium channel 2, isoform B
NM_142892.2	NP_651149.2	inwardly rectifying potassium channel 2, isoform A
NM_001300556.1	NP_001287485.1	inwardly rectifying potassium channel 2, isoform D

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001281	XP_001689370.2
Macaca mulatta (Rhesus monkey)	KCNJ12	XP_002800376.1
Danio rerio (zebrafish)	LOC569146	XP_005162964.1
Pan troglodytes (chimpanzee)	KCNJ12	XP_001154349.1
Caenorhabditis elegans (roundworm)	irk-2	NP_001256977.1
Xenopus tropicalis (tropical clawed frog)	LOC100496092	XP_004917900.1
Homo sapiens (human)	KCNJ12	NP_066292.2
Canis lupus familiaris (dog)	KCNJ12	NP_001229643.1
Rattus norvegicus (Norway rat)	Kcnj12	NP_446433.2
Bos taurus (cattle)	KCNJ12	NP_001019861.2
Mus musculus (house mouse)	Kcnj12	NP_034733.4
Gallus gallus (chicken)	KCNJ12	XP_004945226.1
Drosophila melanogaster (fruit fly)	Irk2	NP_001163700.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001283	XP_003435798.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Irk2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Irk2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05793	NM_142892.2 Latest version!	Drosophila melanogaster inwardly rectifying potassium channel 2, transcript variant A (Irk2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa05794	NM_170076.2 Latest version!	Drosophila melanogaster inwardly rectifying potassium channel 2, transcript variant B (Irk2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa07991	NM_001170229.2 Latest version!	Drosophila melanogaster inwardly rectifying potassium channel 2, transcript variant C (Irk2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa22143	NM_001300556.1 Latest version!	Drosophila melanogaster inwardly rectifying potassium channel 2, transcript variant D (Irk2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Irk2 ( NM_142892.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651149.2 Drosophila melanogaster inwardly rectifying potassium channel 2, transcript variant A (Irk2), mRNA.

Price & Availability↑

CloneID	OFa05793
Clone ID Related Accession (Same CDS sequence)	NM_142892.2
Accession Version	NM_142892.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	inwardly rectifying potassium channel 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Nov 6, 2002 this sequence version replaced NM_142892.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTTTCA ATTTCTCCAC CCACTCGGCG GCCCCCACCT CTACGACCAT ACCAACAACA 
ACACAGGCAA ATGGAGGCGA ACAGGCCACC CAACCGCTCA AGAAGGCCAT CGAAGCGGAT 
CCCGATTCGC TGGACAGTGT GATCAGCAAT CCTCAATCCT ATCCCATCAC AATTGTGCCG 
AATAGGCCGA CCAGCTACTA TGGCCTATCC CAAAATGGCA AGCCGTTCCC CCGCCAACCG 
AGCTGCAGAT CTCGCAATTT CCGGCCTGGC AGCATGAGAC GTGTTCGCCG ACGAGCGGTC 
TTCAAAAATG GTGACTGCAA TGTGGTGCAG AAGCACCTGC AAAGACGCCG AGTTCGTTTC 
CTGCAGGACA TGTACACCAC CATGGTGGAT TGGCAGTGGC GGTGGACTCT CTTGGCCTTC 
GCACTCAGCT TTATCCTGTC TTGGCTCTTC TTTGCTCTCA TCTGGTGGCT GATTATCTAC 
ACACACGGAG ACTTGGAAGA GCCTCACTTG CCAGAAAACC AAGAGGAATC TGGTTGGGCG 
CCTTGTGTTT CGGCCATCGA TGGCTTTACT TCCTGCTTCC TGTTCTCCAT TGAAACGCAG 
CACACTATTG GCTACGGTGT CCGAACCACC TCGCCCGAGT GTCCAGAGGC CATCTTCATG 
ATGTGCTTCC AGTCCATCTA TGGCGTGATG TCTTCAGCAT TTATGGCCGG AATAGTGTTT 
GCCAAGATGA CAAGGGCCAA ACAGCGGGCA CAGACTTTGT TGTTCTCCAA ACACGCCGTA 
ATTTGCCAAC GTGATGGCAC CCTTTCGCTG ATGTTCCGTG TGGGTGACAT GCGAAAATCA 
CACATCATTG GAGCCGGAGT GAGGGCTCAG TTGATCAGGA CAAAGAGCAC CAAGGAGGGT 
GAGGTGATGA CACAGTACTT CACAGAACTG GAGATCGGTA CTGATGACTC CGGCTCTGAT 
TTGTTCTTCA TCTGGCCCAT GGTCATCGAG CACAAGATCG ACGAGAACTC ACCGCTCTAT 
AATCTTAATG CCACTGATAT GCTGCAGGAT AAGTTCGAAA TCGTGGTGAT CCTAGAGGGC 
ACCGTGGAGT CCACTGGACA GAGCACTCAA GCCAGATCCA GCTATATTAA CACTGAGATC 
CTTTGGGGAC ATCGTTTCGA TCCAGTGGTG TTGTACAACA AGGATCTGCA GGCCTATGAA 
ATCGACTACG CTCGCTTTAA CGAAACCACT CAGGTGGACA CTCCGTTGTG CAGTGCTCGG 
GAATTGAACG AGATCTACAA GATCCAGGAG GGATTCCGCA CACCAGAAAC CACCTTTACC 
ATGCGTCAAC TGTCCCACAA TCATTCTGAC CAGGCCTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651149.2
CDS	296..1657
Translation	MRFNFSTHSAAPTSTTIPTTTQANGGEQATQPLKKAIEADPDSLDSVISNPQSYPITIVPNRPTSYYGLSQNGKPFPRQPSCRSRNFRPGSMRRVRRRAVFKNGDCNVVQKHLQRRRVRFLQDMYTTMVDWQWRWTLLAFALSFILSWLFFALIWWLIIYTHGDLEEPHLPENQEESGWAPCVSAIDGFTSCFLFSIETQHTIGYGVRTTSPECPEAIFMMCFQSIYGVMSSAFMAGIVFAKMTRAKQRAQTLLFSKHAVICQRDGTLSLMFRVGDMRKSHIIGAGVRAQLIRTKSTKEGEVMTQYFTELEIGTDDSGSDLFFIWPMVIEHKIDENSPLYNLNATDMLQDKFEIVVILEGTVESTGQSTQARSSYINTEILWGHRFDPVVLYNKDLQAYEIDYARFNETTQVDTPLCSARELNEIYKIQEGFRTPETTFTMRQLSHNHSDQAS

Target ORF information:

RefSeq Version	NM_142892.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster inwardly rectifying potassium channel 2, transcript variant A (Irk2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142892.2

ORF Insert Sequence:

ATGCGTTTCA ATTTCTCCAC CCACTCGGCG GCCCCCACCT CTACGACCAT ACCAACAACA 
ACACAGGCAA ATGGAGGCGA ACAGGCCACC CAACCGCTCA AGAAGGCCAT CGAAGCGGAT 
CCCGATTCGC TGGACAGTGT GATCAGCAAT CCTCAATCCT ATCCCATCAC AATTGTGCCG 
AATAGGCCGA CCAGCTACTA TGGCCTATCC CAAAATGGCA AGCCGTTCCC CCGCCAACCG 
AGCTGCAGAT CTCGCAATTT CCGGCCTGGC AGCATGAGAC GTGTTCGCCG ACGAGCGGTC 
TTCAAAAATG GTGACTGCAA TGTGGTGCAG AAGCACCTGC AAAGACGCCG AGTTCGTTTC 
CTGCAGGACA TGTACACCAC CATGGTGGAT TGGCAGTGGC GGTGGACTCT CTTGGCCTTC 
GCACTCAGCT TTATCCTGTC TTGGCTCTTC TTTGCTCTCA TCTGGTGGCT GATTATCTAC 
ACACACGGAG ACTTGGAAGA GCCTCACTTG CCAGAAAACC AAGAGGAATC TGGTTGGGCG 
CCTTGTGTTT CGGCCATCGA TGGCTTTACT TCCTGCTTCC TGTTCTCCAT TGAAACGCAG 
CACACTATTG GCTACGGTGT CCGAACCACC TCGCCCGAGT GTCCAGAGGC CATCTTCATG 
ATGTGCTTCC AGTCCATCTA TGGCGTGATG TCTTCAGCAT TTATGGCCGG AATAGTGTTT 
GCCAAGATGA CAAGGGCCAA ACAGCGGGCA CAGACTTTGT TGTTCTCCAA ACACGCCGTA 
ATTTGCCAAC GTGATGGCAC CCTTTCGCTG ATGTTCCGTG TGGGTGACAT GCGAAAATCA 
CACATCATTG GAGCCGGAGT GAGGGCTCAG TTGATCAGGA CAAAGAGCAC CAAGGAGGGT 
GAGGTGATGA CACAGTACTT CACAGAACTG GAGATCGGTA CTGATGACTC CGGCTCTGAT 
TTGTTCTTCA TCTGGCCCAT GGTCATCGAG CACAAGATCG ACGAGAACTC ACCGCTCTAT 
AATCTTAATG CCACTGATAT GCTGCAGGAT AAGTTCGAAA TCGTGGTGAT CCTAGAGGGC 
ACCGTGGAGT CCACTGGACA GAGCACTCAA GCCAGATCCA GCTATATTAA CACTGAGATC 
CTTTGGGGAC ATCGTTTCGA TCCAGTGGTG TTGTACAACA AGGATCTGCA GGCCTATGAA 
ATCGACTACG CTCGCTTTAA CGAAACCACT CAGGTGGACA CTCCGTTGTG CAGTGCTCGG 
GAATTGAACG AGATCTACAA GATCCAGGAG GGATTCCGCA CACCAGAAAC CACCTTTACC 
ATGCGTCAAC TGTCCCACAA TCATTCTGAC CAGGCCTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Irk2 ( NM_170076.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732871.1 Drosophila melanogaster inwardly rectifying potassium channel 2, transcript variant B (Irk2), mRNA.

Price & Availability↑

CloneID	OFa05794
Clone ID Related Accession (Same CDS sequence)	NM_170076.2
Accession Version	NM_170076.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	inwardly rectifying potassium channel 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_170076.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGGTCC CGCTCAGCGA TGTTCAGGTC CTGCAGGATC CCACCGACGC GGAAACCCTT 
GTCGGCGGCG ACAAGGAGGA GACCTTTGCC CACATGAAGG AGTGGAAGCG CCAGTACATG 
CGGGGCATCT CGCACACGCC ATCGCAGGCG GGAGTCTACT ACGAGGCCTT GAAGGGTTCC 
AGCCACTCGC TGGCCAAAGG ATCTCGCAAT TTCCGGCCTG GCAGCATGAG ACGTGTTCGC 
CGACGAGCGG TCTTCAAAAA TGGTGACTGC AATGTGGTGC AGAAGCACCT GCAAAGACGC 
CGAGTTCGTT TCCTGCAGGA CATGTACACC ACCATGGTGG ATTGGCAGTG GCGGTGGACT 
CTCTTGGCCT TCGCACTCAG CTTTATCCTG TCTTGGCTCT TCTTTGCTCT CATCTGGTGG 
CTGATTATCT ACACACACGG AGACTTGGAA GAGCCTCACT TGCCAGAAAA CCAAGAGGAA 
TCTGGTTGGG CGCCTTGTGT TTCGGCCATC GATGGCTTTA CTTCCTGCTT CCTGTTCTCC 
ATTGAAACGC AGCACACTAT TGGCTACGGT GTCCGAACCA CCTCGCCCGA GTGTCCAGAG 
GCCATCTTCA TGATGTGCTT CCAGTCCATC TATGGCGTGA TGTCTTCAGC ATTTATGGCC 
GGAATAGTGT TTGCCAAGAT GACAAGGGCC AAACAGCGGG CACAGACTTT GTTGTTCTCC 
AAACACGCCG TAATTTGCCA ACGTGATGGC ACCCTTTCGC TGATGTTCCG TGTGGGTGAC 
ATGCGAAAAT CACACATCAT TGGAGCCGGA GTGAGGGCTC AGTTGATCAG GACAAAGAGC 
ACCAAGGAGG GTGAGGTGAT GACACAGTAC TTCACAGAAC TGGAGATCGG TACTGATGAC 
TCCGGCTCTG ATTTGTTCTT CATCTGGCCC ATGGTCATCG AGCACAAGAT CGACGAGAAC 
TCACCGCTCT ATAATCTTAA TGCCACTGAT ATGCTGCAGG ATAAGTTCGA AATCGTGGTG 
ATCCTAGAGG GCACCGTGGA GTCCACTGGA CAGAGCACTC AAGCCAGATC CAGCTATATT 
AACACTGAGA TCCTTTGGGG ACATCGTTTC GATCCAGTGG TGTTGTACAA CAAGGATCTG 
CAGGCCTATG AAATCGACTA CGCTCGCTTT AACGAAACCA CTCAGGTGGA CACTCCGTTG 
TGCAGTGCTC GGGAATTGAA CGAGATCTAC AAGATCCAGG AGGGATTCCG CACACCAGAA 
ACCACCTTTA CCATGCGTCA ACTGTCCCAC AATCATTCTG ACCAGGCCTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732871.1
CDS	50..1363
Translation	MQVPLSDVQVLQDPTDAETLVGGDKEETFAHMKEWKRQYMRGISHTPSQAGVYYEALKGSSHSLAKGSRNFRPGSMRRVRRRAVFKNGDCNVVQKHLQRRRVRFLQDMYTTMVDWQWRWTLLAFALSFILSWLFFALIWWLIIYTHGDLEEPHLPENQEESGWAPCVSAIDGFTSCFLFSIETQHTIGYGVRTTSPECPEAIFMMCFQSIYGVMSSAFMAGIVFAKMTRAKQRAQTLLFSKHAVICQRDGTLSLMFRVGDMRKSHIIGAGVRAQLIRTKSTKEGEVMTQYFTELEIGTDDSGSDLFFIWPMVIEHKIDENSPLYNLNATDMLQDKFEIVVILEGTVESTGQSTQARSSYINTEILWGHRFDPVVLYNKDLQAYEIDYARFNETTQVDTPLCSARELNEIYKIQEGFRTPETTFTMRQLSHNHSDQAS

Target ORF information:

RefSeq Version	NM_170076.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster inwardly rectifying potassium channel 2, transcript variant B (Irk2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170076.2

ORF Insert Sequence:

ATGCAGGTCC CGCTCAGCGA TGTTCAGGTC CTGCAGGATC CCACCGACGC GGAAACCCTT 
GTCGGCGGCG ACAAGGAGGA GACCTTTGCC CACATGAAGG AGTGGAAGCG CCAGTACATG 
CGGGGCATCT CGCACACGCC ATCGCAGGCG GGAGTCTACT ACGAGGCCTT GAAGGGTTCC 
AGCCACTCGC TGGCCAAAGG ATCTCGCAAT TTCCGGCCTG GCAGCATGAG ACGTGTTCGC 
CGACGAGCGG TCTTCAAAAA TGGTGACTGC AATGTGGTGC AGAAGCACCT GCAAAGACGC 
CGAGTTCGTT TCCTGCAGGA CATGTACACC ACCATGGTGG ATTGGCAGTG GCGGTGGACT 
CTCTTGGCCT TCGCACTCAG CTTTATCCTG TCTTGGCTCT TCTTTGCTCT CATCTGGTGG 
CTGATTATCT ACACACACGG AGACTTGGAA GAGCCTCACT TGCCAGAAAA CCAAGAGGAA 
TCTGGTTGGG CGCCTTGTGT TTCGGCCATC GATGGCTTTA CTTCCTGCTT CCTGTTCTCC 
ATTGAAACGC AGCACACTAT TGGCTACGGT GTCCGAACCA CCTCGCCCGA GTGTCCAGAG 
GCCATCTTCA TGATGTGCTT CCAGTCCATC TATGGCGTGA TGTCTTCAGC ATTTATGGCC 
GGAATAGTGT TTGCCAAGAT GACAAGGGCC AAACAGCGGG CACAGACTTT GTTGTTCTCC 
AAACACGCCG TAATTTGCCA ACGTGATGGC ACCCTTTCGC TGATGTTCCG TGTGGGTGAC 
ATGCGAAAAT CACACATCAT TGGAGCCGGA GTGAGGGCTC AGTTGATCAG GACAAAGAGC 
ACCAAGGAGG GTGAGGTGAT GACACAGTAC TTCACAGAAC TGGAGATCGG TACTGATGAC 
TCCGGCTCTG ATTTGTTCTT CATCTGGCCC ATGGTCATCG AGCACAAGAT CGACGAGAAC 
TCACCGCTCT ATAATCTTAA TGCCACTGAT ATGCTGCAGG ATAAGTTCGA AATCGTGGTG 
ATCCTAGAGG GCACCGTGGA GTCCACTGGA CAGAGCACTC AAGCCAGATC CAGCTATATT 
AACACTGAGA TCCTTTGGGG ACATCGTTTC GATCCAGTGG TGTTGTACAA CAAGGATCTG 
CAGGCCTATG AAATCGACTA CGCTCGCTTT AACGAAACCA CTCAGGTGGA CACTCCGTTG 
TGCAGTGCTC GGGAATTGAA CGAGATCTAC AAGATCCAGG AGGGATTCCG CACACCAGAA 
ACCACCTTTA CCATGCGTCA ACTGTCCCAC AATCATTCTG ACCAGGCCTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Irk2 ( NM_001170229.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163700.1 Drosophila melanogaster inwardly rectifying potassium channel 2, transcript variant C (Irk2), mRNA.

Price & Availability↑

CloneID	OFa07991
Clone ID Related Accession (Same CDS sequence)	NM_001170229.2
Accession Version	NM_001170229.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	inwardly rectifying potassium channel 2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001170229.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGGTCC CGCTCAGCGA TGTTCAGGTC CTGCAGGATC CCACCGACGC GGAAACCCTT 
GTCGGCGGCG ACAAGGAGGA GACCTTTGCC CACATGAAGG AGTGGAAGCG CCAGTACATG 
CGGGGCATCT CGCACACGCC ATCGCAGGCG GGAGTCTACT ACGAGGCCTT GAAGGGTTCC 
AGCCACTCGC TGGCCAAAGG ATCTCGCAAT TTCCGGCCTG GCAGCATGAG ACGTGTTCGC 
CGACGAGCGG TCTTCAAAAA TGGTGACTGC AATGTGGTGC AGAAGCACCT GCAAAGACGC 
CGAGTTCGTT TCCTGCAGGA CATGTACACC ACCATGGTGG ATTGGCAGTG GCGGTGGACT 
CTCTTGGCCT TCGCACTCAG CTTTATCCTG TCTTGGCTCT TCTTTGCTCT CATCTGGTGG 
CTGATTATCT ACACACACGG AGACTTGGAA GAGCCTCACT TGCCAGAAAA CCAAGAGGAA 
TCTGGTTGGG CGCCTTGTGT TTCGGCCATC GATGGCTTTA CTTCCTGCTT CCTGTTCTCC 
ATTGAAACGC AGCACACTAT TGGCTACGGT GTCCGAACCA CCTCGCCCGA GTGTCCAGAG 
GCCATCTTCA TGATGTGCTT CCAGTCCATC TATGGCGTGA TGTCTTCAGC ATTTATGGCC 
GGAATAGTGT TTGCCAAGAT GACAAGGGCC AAACAGCGGG CACAGACTTT GTTGTTCTCC 
AAACACGCCG TAATTTGCCA ACGTGATGGC ACCCTTTCGC TGATGTTCCG TGTGGGTGAC 
ATGCGAAAAT CACACATCAT TGGAGCCGGA GTGAGGGCTC AGTTGATCAG GACAAAGAGC 
ACCAAGGAGG GTGAGGTGAT GACACAGTAC TTCACAGAAC TGGAGATCGG TACTGATGAC 
TCCGGCTCTG ATTTGTTCTT CATCTGGCCC ATGGTCATCG AGCACAAGAT CGACGAGAAC 
TCACCGCTCT ATAATCTTAA TGCCACTGAT ATGCTGCAGG ATAAGTTCGA AATCGTGGTG 
ATCCTAGAGG GCACCGTGGA GTCCACTGGA CAGAGCACTC AAGCCAGATC CAGCTATATT 
AACACTGAGA TCCTTTGGGG ACATCGTTTC GATCCAGTGG TGTTGTACAA CAAGGATCTG 
CAGGCCTATG AAATCGACTA CGCTCGCTTT AACGAAACCA CTCAGGTGGA CACTCCGTTG 
TGCAGTGCTC GGGAATTGAA CGAGATCTAC AAGATCCAGG AGGGATTCCG CACACCAGCT 
TCCCCATCGA CCCAATCGAG GTGCTCCTGC AGTTCCGGCT ATCAGACACC TGGAAGGCAA 
AGCCGCCTGC GTTGGCAGCT CTCGGTGCCC TTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163700.1
CDS	50..1405
Translation	MQVPLSDVQVLQDPTDAETLVGGDKEETFAHMKEWKRQYMRGISHTPSQAGVYYEALKGSSHSLAKGSRNFRPGSMRRVRRRAVFKNGDCNVVQKHLQRRRVRFLQDMYTTMVDWQWRWTLLAFALSFILSWLFFALIWWLIIYTHGDLEEPHLPENQEESGWAPCVSAIDGFTSCFLFSIETQHTIGYGVRTTSPECPEAIFMMCFQSIYGVMSSAFMAGIVFAKMTRAKQRAQTLLFSKHAVICQRDGTLSLMFRVGDMRKSHIIGAGVRAQLIRTKSTKEGEVMTQYFTELEIGTDDSGSDLFFIWPMVIEHKIDENSPLYNLNATDMLQDKFEIVVILEGTVESTGQSTQARSSYINTEILWGHRFDPVVLYNKDLQAYEIDYARFNETTQVDTPLCSARELNEIYKIQEGFRTPASPSTQSRCSCSSGYQTPGRQSRLRWQLSVPL

Target ORF information:

RefSeq Version	NM_001170229.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster inwardly rectifying potassium channel 2, transcript variant C (Irk2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170229.2

ORF Insert Sequence:

ATGCAGGTCC CGCTCAGCGA TGTTCAGGTC CTGCAGGATC CCACCGACGC GGAAACCCTT 
GTCGGCGGCG ACAAGGAGGA GACCTTTGCC CACATGAAGG AGTGGAAGCG CCAGTACATG 
CGGGGCATCT CGCACACGCC ATCGCAGGCG GGAGTCTACT ACGAGGCCTT GAAGGGTTCC 
AGCCACTCGC TGGCCAAAGG ATCTCGCAAT TTCCGGCCTG GCAGCATGAG ACGTGTTCGC 
CGACGAGCGG TCTTCAAAAA TGGTGACTGC AATGTGGTGC AGAAGCACCT GCAAAGACGC 
CGAGTTCGTT TCCTGCAGGA CATGTACACC ACCATGGTGG ATTGGCAGTG GCGGTGGACT 
CTCTTGGCCT TCGCACTCAG CTTTATCCTG TCTTGGCTCT TCTTTGCTCT CATCTGGTGG 
CTGATTATCT ACACACACGG AGACTTGGAA GAGCCTCACT TGCCAGAAAA CCAAGAGGAA 
TCTGGTTGGG CGCCTTGTGT TTCGGCCATC GATGGCTTTA CTTCCTGCTT CCTGTTCTCC 
ATTGAAACGC AGCACACTAT TGGCTACGGT GTCCGAACCA CCTCGCCCGA GTGTCCAGAG 
GCCATCTTCA TGATGTGCTT CCAGTCCATC TATGGCGTGA TGTCTTCAGC ATTTATGGCC 
GGAATAGTGT TTGCCAAGAT GACAAGGGCC AAACAGCGGG CACAGACTTT GTTGTTCTCC 
AAACACGCCG TAATTTGCCA ACGTGATGGC ACCCTTTCGC TGATGTTCCG TGTGGGTGAC 
ATGCGAAAAT CACACATCAT TGGAGCCGGA GTGAGGGCTC AGTTGATCAG GACAAAGAGC 
ACCAAGGAGG GTGAGGTGAT GACACAGTAC TTCACAGAAC TGGAGATCGG TACTGATGAC 
TCCGGCTCTG ATTTGTTCTT CATCTGGCCC ATGGTCATCG AGCACAAGAT CGACGAGAAC 
TCACCGCTCT ATAATCTTAA TGCCACTGAT ATGCTGCAGG ATAAGTTCGA AATCGTGGTG 
ATCCTAGAGG GCACCGTGGA GTCCACTGGA CAGAGCACTC AAGCCAGATC CAGCTATATT 
AACACTGAGA TCCTTTGGGG ACATCGTTTC GATCCAGTGG TGTTGTACAA CAAGGATCTG 
CAGGCCTATG AAATCGACTA CGCTCGCTTT AACGAAACCA CTCAGGTGGA CACTCCGTTG 
TGCAGTGCTC GGGAATTGAA CGAGATCTAC AAGATCCAGG AGGGATTCCG CACACCAGCT 
TCCCCATCGA CCCAATCGAG GTGCTCCTGC AGTTCCGGCT ATCAGACACC TGGAAGGCAA 
AGCCGCCTGC GTTGGCAGCT CTCGGTGCCC TTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Irk2 ( NM_001300556.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287485.1 Drosophila melanogaster inwardly rectifying potassium channel 2, transcript variant D (Irk2), mRNA.

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CloneID	OFa22143
Clone ID Related Accession (Same CDS sequence)	NM_001300556.1
Accession Version	NM_001300556.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	inwardly rectifying potassium channel 2, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTTTCA ATTTCTCCAC CCACTCGGCG GCCCCCACCT CTACGACCAT ACCAACAACA 
ACACAGGCAA ATGGAGGCGA ACAGGCCACC CAACCGCTCA AGAAGGCCAT CGAAGCGGAT 
CCCGATTCGC TGGACAGTGT GATCAGCAAT CCTCAATCCT ATCCCATCAC AATTGTGCCG 
AATAGGCCGA CCAGCTACTA TGGCCTATCC CAAAATGGCA AGCCGTTCCC CCGCCAACCG 
AGCTGCAGAT CTCGCAATTT CCGGCCTGGC AGCATGAGAC GTGTTCGCCG ACGAGCGGTC 
TTCAAAAATG GTGACTGCAA TGTGGTGCAG AAGCACCTGC AAAGACGCCG AGTTCGTTTC 
CTGCAGGACA TGTACACCAC CATGGTGGAT TGGCAGTGGC GGTGGACTCT CTTGGCCTTC 
GCACTCAGCT TTATCCTGTC TTGGCTCTTC TTTGCTCTCA TCTGGTGGCT GATTATCTAC 
ACACACGGAG ACTTGGAAGA GCCTCACTTG CCAGAAAACC AAGAGGAATC TGGTTGGGCG 
CCTTGTGTTT CGGCCATCGA TGGCTTTACT TCCTGCTTCC TGTTCTCCAT TGAAACGCAG 
CACACTATTG GCTACGGTGT CCGAACCACC TCGCCCGAGT GTCCAGAGGC CATCTTCATG 
ATGTGCTTCC AGTCCATCTA TGGCGTGATG TCTTCAGCAT TTATGGCCGG AATAGTGTTT 
GCCAAGATGA CAAGGGCCAA ACAGCGGGCA CAGACTTTGT TGTTCTCCAA ACACGCCGTA 
ATTTGCCAAC GTGATGGCAC CCTTTCGCTG ATGTTCCGTG TGGGTGACAT GCGAAAATCA 
CACATCATTG GAGCCGGAGT GAGGGCTCAG TTGATCAGGA CAAAGAGCAC CAAGGAGGGT 
GAGGTGATGA CACAGTACTT CACAGAACTG GAGATCGGTA CTGATGACTC CGGCTCTGAT 
TTGTTCTTCA TCTGGCCCAT GGTCATCGAG CACAAGATCG ACGAGAACTC ACCGCTCTAT 
AATCTTAATG CCACTGATAT GCTGCAGGAT AAGTTCGAAA TCGTGGTGAT CCTAGAGGGC 
ACCGTGGAGT CCACTGGACA GAGCACTCAA GCCAGATCCA GCTATATTAA CACTGAGATC 
CTTTGGGGAC ATCGTTTCGA TCCAGTGGTG TTGTACAACA AGGATCTGCA GGCCTATGAA 
ATCGACTACG CTCGCTTTAA CGAAACCACT CAGGTGGACA CTCCGTTGTG CAGTGCTCGG 
GAATTGAACG AGATCTACAA GATCCAGGAG GGATTCCGCA CACCAGCAGA AACCACCTTT 
ACCATGCGTC AACTGTCCCA CAATCATTCT GACCAGGCCT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287485.1
CDS	296..1660
Translation	MRFNFSTHSAAPTSTTIPTTTQANGGEQATQPLKKAIEADPDSLDSVISNPQSYPITIVPNRPTSYYGLSQNGKPFPRQPSCRSRNFRPGSMRRVRRRAVFKNGDCNVVQKHLQRRRVRFLQDMYTTMVDWQWRWTLLAFALSFILSWLFFALIWWLIIYTHGDLEEPHLPENQEESGWAPCVSAIDGFTSCFLFSIETQHTIGYGVRTTSPECPEAIFMMCFQSIYGVMSSAFMAGIVFAKMTRAKQRAQTLLFSKHAVICQRDGTLSLMFRVGDMRKSHIIGAGVRAQLIRTKSTKEGEVMTQYFTELEIGTDDSGSDLFFIWPMVIEHKIDENSPLYNLNATDMLQDKFEIVVILEGTVESTGQSTQARSSYINTEILWGHRFDPVVLYNKDLQAYEIDYARFNETTQVDTPLCSARELNEIYKIQEGFRTPAETTFTMRQLSHNHSDQAS

Target ORF information:

RefSeq Version	NM_001300556.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster inwardly rectifying potassium channel 2, transcript variant D (Irk2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300556.1

ORF Insert Sequence:

ATGCGTTTCA ATTTCTCCAC CCACTCGGCG GCCCCCACCT CTACGACCAT ACCAACAACA 
ACACAGGCAA ATGGAGGCGA ACAGGCCACC CAACCGCTCA AGAAGGCCAT CGAAGCGGAT 
CCCGATTCGC TGGACAGTGT GATCAGCAAT CCTCAATCCT ATCCCATCAC AATTGTGCCG 
AATAGGCCGA CCAGCTACTA TGGCCTATCC CAAAATGGCA AGCCGTTCCC CCGCCAACCG 
AGCTGCAGAT CTCGCAATTT CCGGCCTGGC AGCATGAGAC GTGTTCGCCG ACGAGCGGTC 
TTCAAAAATG GTGACTGCAA TGTGGTGCAG AAGCACCTGC AAAGACGCCG AGTTCGTTTC 
CTGCAGGACA TGTACACCAC CATGGTGGAT TGGCAGTGGC GGTGGACTCT CTTGGCCTTC 
GCACTCAGCT TTATCCTGTC TTGGCTCTTC TTTGCTCTCA TCTGGTGGCT GATTATCTAC 
ACACACGGAG ACTTGGAAGA GCCTCACTTG CCAGAAAACC AAGAGGAATC TGGTTGGGCG 
CCTTGTGTTT CGGCCATCGA TGGCTTTACT TCCTGCTTCC TGTTCTCCAT TGAAACGCAG 
CACACTATTG GCTACGGTGT CCGAACCACC TCGCCCGAGT GTCCAGAGGC CATCTTCATG 
ATGTGCTTCC AGTCCATCTA TGGCGTGATG TCTTCAGCAT TTATGGCCGG AATAGTGTTT 
GCCAAGATGA CAAGGGCCAA ACAGCGGGCA CAGACTTTGT TGTTCTCCAA ACACGCCGTA 
ATTTGCCAAC GTGATGGCAC CCTTTCGCTG ATGTTCCGTG TGGGTGACAT GCGAAAATCA 
CACATCATTG GAGCCGGAGT GAGGGCTCAG TTGATCAGGA CAAAGAGCAC CAAGGAGGGT 
GAGGTGATGA CACAGTACTT CACAGAACTG GAGATCGGTA CTGATGACTC CGGCTCTGAT 
TTGTTCTTCA TCTGGCCCAT GGTCATCGAG CACAAGATCG ACGAGAACTC ACCGCTCTAT 
AATCTTAATG CCACTGATAT GCTGCAGGAT AAGTTCGAAA TCGTGGTGAT CCTAGAGGGC 
ACCGTGGAGT CCACTGGACA GAGCACTCAA GCCAGATCCA GCTATATTAA CACTGAGATC 
CTTTGGGGAC ATCGTTTCGA TCCAGTGGTG TTGTACAACA AGGATCTGCA GGCCTATGAA 
ATCGACTACG CTCGCTTTAA CGAAACCACT CAGGTGGACA CTCCGTTGTG CAGTGCTCGG 
GAATTGAACG AGATCTACAA GATCCAGGAG GGATTCCGCA CACCAGCAGA AACCACCTTT 
ACCATGCGTC AACTGTCCCA CAATCATTCT GACCAGGCCT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Irk2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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