PyK cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	PyK
Entrez Gene ID	42620
Full Name	Pyruvate kinase
Synonyms	CG7070,Dmel\CG7070,PK,PYK,Pk,Pyk,l(3)rQ178,l(3)r[Q178]
General protein information	Preferred Names Pyruvate kinase Names CG7070 gene product from transcript CG7070-RB CG7070-PA CG7070-PB PyK-PA PyK-PB lethal (3) rQ178 pyruvate kinase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 94A9-94A6

mRNA and Protein(s)

mRNA	Protein	Name
NM_079724.3	NP_524448.3	pyruvate kinase, isoform A
NM_170004.2	NP_732723.1	pyruvate kinase, isoform B

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	pyk1	NP_594346.1
Homo sapiens (human)	PKM	NP_001193725.1
Gallus gallus (chicken)	PKM2	NP_990800.1
Drosophila melanogaster (fruit fly)	PyK	NP_524448.3
Arabidopsis thaliana (thale cress)	AT5G63680	NP_201173.1
Arabidopsis thaliana (thale cress)	AT5G08570	NP_196474.1
Kluyveromyces lactis	KLLA0F23397g	XP_456122.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004596	XP_315228.5
Magnaporthe oryzae (rice blast fungus)	MGG_08063	XP_003715015.1
Macaca mulatta (Rhesus monkey)	PKM	XP_001099473.2
Rattus norvegicus (Norway rat)	Pkm	NP_445749.1
Oryza sativa (rice)	Os04g0677500	NP_001054265.1
Neurospora crassa	NCU06075	XP_959838.1
Pan troglodytes (chimpanzee)	PKM	XP_003314790.1
Canis lupus familiaris (dog)	PKM	XP_005638614.1
Caenorhabditis elegans (roundworm)	pyk-1	NP_001251014.1
Caenorhabditis elegans (roundworm)	pyk-2	NP_001255515.1
Saccharomyces cerevisiae (baker's yeast)	CDC19	NP_009362.1
Eremothecium gossypii	AGOS_ADR368W	NP_984464.1
Bos taurus (cattle)	PKM	NP_001192656.1
Mus musculus (house mouse)	Pkm	NP_035229.2
Danio rerio (zebrafish)	pkma	NP_955365.1
Xenopus tropicalis (tropical clawed frog)	pkm	NP_001072532.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PyK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PyK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05680	NM_170004.2 Latest version!	Drosophila melanogaster pyruvate kinase, transcript variant B (PyK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OFa05681	NM_079724.3 Latest version!	Drosophila melanogaster pyruvate kinase, transcript variant A (PyK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PyK ( NM_170004.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732723.1 Drosophila melanogaster pyruvate kinase, transcript variant B (PyK), mRNA.

Price & Availability↑

CloneID	OFa05680
Clone ID Related Accession (Same CDS sequence)	NM_170004.2
Accession Version	NM_170004.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	pyruvate kinase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_170004.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGCAG GAGCTGATAC CCAACTGGAG CACATGTGCC GTCTGCAGTT CGACTCGCCA 
GTGCCCCATG TGCGTCTGTC CGGAATCGTG TGCACCATCG GACCTGCCTC CAGCAGCGTG 
GAGATGCTGG AGAAGATGAT GGCCACCGGC ATGAACATCG CGCGCATGAA CTTCTCTCAC 
GGATCGCACG AGTACCATGC CGCCACCGTG GCCAATGTGC GCCAGGCGGT GAAGAACTAC 
TCGGCCAAGC TGGGCTACGA ACACCCCGTG GCCATTGCCC TGGACACCAA GGGGCCCGAG 
ATCCGTACCG GTCTGATCGG AGGCAGCGGC ACCGCCGAGA TTGAGCTGAA GAAGGGCGAG 
AAGATCAAGC TGACCACCAA CAAGGAATTC CTGGAGAAGG GCTCTCTGGA GATTGTGTAC 
GTGGACTACG AGAACATTGT CAATGTGGTG AAGCCCGGCA ACCGGGTGTT CGTCGATGAC 
GGTCTGATCT CACTGATTGT CCGCGAGGTA GGCAAGGATT CCCTCACCTG CGAGGTGGAG 
AACGGCGGCT CTCTGGGTTC CCGCAAGGGT GTGAACCTGC CAGGCGTGCC CGTCGATCTG 
CCTGCCGTCT CCGAGAAGGA TAAGAGCGAT CTGCTGTTCG GTGTGGAGCA GGAAGTGGAC 
ATGATCTTTG CTTCGTTCAT CCGCAACGCC GCTGCTTTGA CCGAGATCCG TAAGGTTCTT 
GGCGAGAAGG GCAAGAACAT CAAGATCATT TCCAAGATCG AGAACCAGCA GGGCATGCAC 
AACCTGGACG AGATCATCGA GGCCGGTGAT GGCATTATGG TGGCCCGTGG AGATCTGGGT 
ATTGAGATTC CCGCCGAGAA GGTGTTCCTC GCCCAGAAGG CCATGATTGC CCGCTGCAAC 
AAGGCTGGCA AGCCTGTGAT CTGCGCCACT CAGATGTTGG AGTCAATGGT GAAGAAGCCA 
CGTCCCACTC GCGCTGAGAT CTCTGATGTG GCCAACGCTG TGCTCGATGG TGCTGATTGC 
GTCATGTTGT CTGGTGAGAC CGCCAAGGGC GAGTACCCGC TGGAGTGCGT CCTGACCATG 
GCCAAGACCT GCAAGGAGGC CGAGGCTGCC CTCTGGCACC AGAACCTCTT TAACGACTTG 
GTTCGCGGCG CTGGTACCAT CGATGCCTCT CACGCGGCTG CCATCGCTGC CGTTGAGGCT 
GCCACCAAGG CCAAGGCCTC CGCCATCGTG GTGATCACCA CCAGCGGCAA GTCGGCCTTC 
CAGGTGAGCA AGTACCGCCC ACGCTGCCCC ATCATCGCGG TCACCCGTTT CGCGCAGACC 
GCCCGACAGG CCCATCTCTA CCGTGGACTG GTGCCACTCA TCTACAAGGA GCCCGGTCTT 
GGTGACTGGC TGAAGGACGT GGACGTGCGC GTGCAGTTCG GTCTGCAGGT CGGAAAGAAG 
AACGGCTTCA TCAAGACCGG CGATTCCGTC GTGGTGGTTA CCGGCTGGAA GCAGGGCTCC 
GGCTTCACCA ACACCATCCG CATTGTCACC GTCGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732723.1
CDS	155..1693
Translation	MAAGADTQLEHMCRLQFDSPVPHVRLSGIVCTIGPASSSVEMLEKMMATGMNIARMNFSHGSHEYHAATVANVRQAVKNYSAKLGYEHPVAIALDTKGPEIRTGLIGGSGTAEIELKKGEKIKLTTNKEFLEKGSLEIVYVDYENIVNVVKPGNRVFVDDGLISLIVREVGKDSLTCEVENGGSLGSRKGVNLPGVPVDLPAVSEKDKSDLLFGVEQEVDMIFASFIRNAAALTEIRKVLGEKGKNIKIISKIENQQGMHNLDEIIEAGDGIMVARGDLGIEIPAEKVFLAQKAMIARCNKAGKPVICATQMLESMVKKPRPTRAEISDVANAVLDGADCVMLSGETAKGEYPLECVLTMAKTCKEAEAALWHQNLFNDLVRGAGTIDASHAAAIAAVEAATKAKASAIVVITTSGKSAFQVSKYRPRCPIIAVTRFAQTARQAHLYRGLVPLIYKEPGLGDWLKDVDVRVQFGLQVGKKNGFIKTGDSVVVVTGWKQGSGFTNTIRIVTVE

Target ORF information:

RefSeq Version	NM_170004.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pyruvate kinase, transcript variant B (PyK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170004.2

ORF Insert Sequence:

ATGGCCGCAG GAGCTGATAC CCAACTGGAG CACATGTGCC GTCTGCAGTT CGACTCGCCA 
GTGCCCCATG TGCGTCTGTC CGGAATCGTG TGCACCATCG GACCTGCCTC CAGCAGCGTG 
GAGATGCTGG AGAAGATGAT GGCCACCGGC ATGAACATCG CGCGCATGAA CTTCTCTCAC 
GGATCGCACG AGTACCATGC CGCCACCGTG GCCAATGTGC GCCAGGCGGT GAAGAACTAC 
TCGGCCAAGC TGGGCTACGA ACACCCCGTG GCCATTGCCC TGGACACCAA GGGGCCCGAG 
ATCCGTACCG GTCTGATCGG AGGCAGCGGC ACCGCCGAGA TTGAGCTGAA GAAGGGCGAG 
AAGATCAAGC TGACCACCAA CAAGGAATTC CTGGAGAAGG GCTCTCTGGA GATTGTGTAC 
GTGGACTACG AGAACATTGT CAATGTGGTG AAGCCCGGCA ACCGGGTGTT CGTCGATGAC 
GGTCTGATCT CACTGATTGT CCGCGAGGTA GGCAAGGATT CCCTCACCTG CGAGGTGGAG 
AACGGCGGCT CTCTGGGTTC CCGCAAGGGT GTGAACCTGC CAGGCGTGCC CGTCGATCTG 
CCTGCCGTCT CCGAGAAGGA TAAGAGCGAT CTGCTGTTCG GTGTGGAGCA GGAAGTGGAC 
ATGATCTTTG CTTCGTTCAT CCGCAACGCC GCTGCTTTGA CCGAGATCCG TAAGGTTCTT 
GGCGAGAAGG GCAAGAACAT CAAGATCATT TCCAAGATCG AGAACCAGCA GGGCATGCAC 
AACCTGGACG AGATCATCGA GGCCGGTGAT GGCATTATGG TGGCCCGTGG AGATCTGGGT 
ATTGAGATTC CCGCCGAGAA GGTGTTCCTC GCCCAGAAGG CCATGATTGC CCGCTGCAAC 
AAGGCTGGCA AGCCTGTGAT CTGCGCCACT CAGATGTTGG AGTCAATGGT GAAGAAGCCA 
CGTCCCACTC GCGCTGAGAT CTCTGATGTG GCCAACGCTG TGCTCGATGG TGCTGATTGC 
GTCATGTTGT CTGGTGAGAC CGCCAAGGGC GAGTACCCGC TGGAGTGCGT CCTGACCATG 
GCCAAGACCT GCAAGGAGGC CGAGGCTGCC CTCTGGCACC AGAACCTCTT TAACGACTTG 
GTTCGCGGCG CTGGTACCAT CGATGCCTCT CACGCGGCTG CCATCGCTGC CGTTGAGGCT 
GCCACCAAGG CCAAGGCCTC CGCCATCGTG GTGATCACCA CCAGCGGCAA GTCGGCCTTC 
CAGGTGAGCA AGTACCGCCC ACGCTGCCCC ATCATCGCGG TCACCCGTTT CGCGCAGACC 
GCCCGACAGG CCCATCTCTA CCGTGGACTG GTGCCACTCA TCTACAAGGA GCCCGGTCTT 
GGTGACTGGC TGAAGGACGT GGACGTGCGC GTGCAGTTCG GTCTGCAGGT CGGAAAGAAG 
AACGGCTTCA TCAAGACCGG CGATTCCGTC GTGGTGGTTA CCGGCTGGAA GCAGGGCTCC 
GGCTTCACCA ACACCATCCG CATTGTCACC GTCGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PyK ( NM_079724.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524448.3 Drosophila melanogaster pyruvate kinase, transcript variant A (PyK), mRNA.

Price & Availability↑

CloneID	OFa05681
Clone ID Related Accession (Same CDS sequence)	NM_079724.3
Accession Version	NM_079724.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	pyruvate kinase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_079724.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGAACG TAACCATCTA CGATGAGGCA CCGCAGCTGA AGCCCAACGA AGTACCCCAA 
AACATGGCCG CAGGAGCTGA TACCCAACTG GAGCACATGT GCCGTCTGCA GTTCGACTCG 
CCAGTGCCCC ATGTGCGTCT GTCCGGAATC GTGTGCACCA TCGGACCTGC CTCCAGCAGC 
GTGGAGATGC TGGAGAAGAT GATGGCCACC GGCATGAACA TCGCGCGCAT GAACTTCTCT 
CACGGATCGC ACGAGTACCA TGCCGCCACC GTGGCCAATG TGCGCCAGGC GGTGAAGAAC 
TACTCGGCCA AGCTGGGCTA CGAACACCCC GTGGCCATTG CCCTGGACAC CAAGGGGCCC 
GAGATCCGTA CCGGTCTGAT CGGAGGCAGC GGCACCGCCG AGATTGAGCT GAAGAAGGGC 
GAGAAGATCA AGCTGACCAC CAACAAGGAA TTCCTGGAGA AGGGCTCTCT GGAGATTGTG 
TACGTGGACT ACGAGAACAT TGTCAATGTG GTGAAGCCCG GCAACCGGGT GTTCGTCGAT 
GACGGTCTGA TCTCACTGAT TGTCCGCGAG GTAGGCAAGG ATTCCCTCAC CTGCGAGGTG 
GAGAACGGCG GCTCTCTGGG TTCCCGCAAG GGTGTGAACC TGCCAGGCGT GCCCGTCGAT 
CTGCCTGCCG TCTCCGAGAA GGATAAGAGC GATCTGCTGT TCGGTGTGGA GCAGGAAGTG 
GACATGATCT TTGCTTCGTT CATCCGCAAC GCCGCTGCTT TGACCGAGAT CCGTAAGGTT 
CTTGGCGAGA AGGGCAAGAA CATCAAGATC ATTTCCAAGA TCGAGAACCA GCAGGGCATG 
CACAACCTGG ACGAGATCAT CGAGGCCGGT GATGGCATTA TGGTGGCCCG TGGAGATCTG 
GGTATTGAGA TTCCCGCCGA GAAGGTGTTC CTCGCCCAGA AGGCCATGAT TGCCCGCTGC 
AACAAGGCTG GCAAGCCTGT GATCTGCGCC ACTCAGATGT TGGAGTCAAT GGTGAAGAAG 
CCACGTCCCA CTCGCGCTGA GATCTCTGAT GTGGCCAACG CTGTGCTCGA TGGTGCTGAT 
TGCGTCATGT TGTCTGGTGA GACCGCCAAG GGCGAGTACC CGCTGGAGTG CGTCCTGACC 
ATGGCCAAGA CCTGCAAGGA GGCCGAGGCT GCCCTCTGGC ACCAGAACCT CTTTAACGAC 
TTGGTTCGCG GCGCTGGTAC CATCGATGCC TCTCACGCGG CTGCCATCGC TGCCGTTGAG 
GCTGCCACCA AGGCCAAGGC CTCCGCCATC GTGGTGATCA CCACCAGCGG CAAGTCGGCC 
TTCCAGGTGA GCAAGTACCG CCCACGCTGC CCCATCATCG CGGTCACCCG TTTCGCGCAG 
ACCGCCCGAC AGGCCCATCT CTACCGTGGA CTGGTGCCAC TCATCTACAA GGAGCCCGGT 
CTTGGTGACT GGCTGAAGGA CGTGGACGTG CGCGTGCAGT TCGGTCTGCA GGTCGGAAAG 
AAGAACGGCT TCATCAAGAC CGGCGATTCC GTCGTGGTGG TTACCGGCTG GAAGCAGGGC 
TCCGGCTTCA CCAACACCAT CCGCATTGTC ACCGTCGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524448.3
CDS	243..1844
Translation	MVNVTIYDEAPQLKPNEVPQNMAAGADTQLEHMCRLQFDSPVPHVRLSGIVCTIGPASSSVEMLEKMMATGMNIARMNFSHGSHEYHAATVANVRQAVKNYSAKLGYEHPVAIALDTKGPEIRTGLIGGSGTAEIELKKGEKIKLTTNKEFLEKGSLEIVYVDYENIVNVVKPGNRVFVDDGLISLIVREVGKDSLTCEVENGGSLGSRKGVNLPGVPVDLPAVSEKDKSDLLFGVEQEVDMIFASFIRNAAALTEIRKVLGEKGKNIKIISKIENQQGMHNLDEIIEAGDGIMVARGDLGIEIPAEKVFLAQKAMIARCNKAGKPVICATQMLESMVKKPRPTRAEISDVANAVLDGADCVMLSGETAKGEYPLECVLTMAKTCKEAEAALWHQNLFNDLVRGAGTIDASHAAAIAAVEAATKAKASAIVVITTSGKSAFQVSKYRPRCPIIAVTRFAQTARQAHLYRGLVPLIYKEPGLGDWLKDVDVRVQFGLQVGKKNGFIKTGDSVVVVTGWKQGSGFTNTIRIVTVE

Target ORF information:

RefSeq Version	NM_079724.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pyruvate kinase, transcript variant A (PyK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079724.3

ORF Insert Sequence:

ATGGTGAACG TAACCATCTA CGATGAGGCA CCGCAGCTGA AGCCCAACGA AGTACCCCAA 
AACATGGCCG CAGGAGCTGA TACCCAACTG GAGCACATGT GCCGTCTGCA GTTCGACTCG 
CCAGTGCCCC ATGTGCGTCT GTCCGGAATC GTGTGCACCA TCGGACCTGC CTCCAGCAGC 
GTGGAGATGC TGGAGAAGAT GATGGCCACC GGCATGAACA TCGCGCGCAT GAACTTCTCT 
CACGGATCGC ACGAGTACCA TGCCGCCACC GTGGCCAATG TGCGCCAGGC GGTGAAGAAC 
TACTCGGCCA AGCTGGGCTA CGAACACCCC GTGGCCATTG CCCTGGACAC CAAGGGGCCC 
GAGATCCGTA CCGGTCTGAT CGGAGGCAGC GGCACCGCCG AGATTGAGCT GAAGAAGGGC 
GAGAAGATCA AGCTGACCAC CAACAAGGAA TTCCTGGAGA AGGGCTCTCT GGAGATTGTG 
TACGTGGACT ACGAGAACAT TGTCAATGTG GTGAAGCCCG GCAACCGGGT GTTCGTCGAT 
GACGGTCTGA TCTCACTGAT TGTCCGCGAG GTAGGCAAGG ATTCCCTCAC CTGCGAGGTG 
GAGAACGGCG GCTCTCTGGG TTCCCGCAAG GGTGTGAACC TGCCAGGCGT GCCCGTCGAT 
CTGCCTGCCG TCTCCGAGAA GGATAAGAGC GATCTGCTGT TCGGTGTGGA GCAGGAAGTG 
GACATGATCT TTGCTTCGTT CATCCGCAAC GCCGCTGCTT TGACCGAGAT CCGTAAGGTT 
CTTGGCGAGA AGGGCAAGAA CATCAAGATC ATTTCCAAGA TCGAGAACCA GCAGGGCATG 
CACAACCTGG ACGAGATCAT CGAGGCCGGT GATGGCATTA TGGTGGCCCG TGGAGATCTG 
GGTATTGAGA TTCCCGCCGA GAAGGTGTTC CTCGCCCAGA AGGCCATGAT TGCCCGCTGC 
AACAAGGCTG GCAAGCCTGT GATCTGCGCC ACTCAGATGT TGGAGTCAAT GGTGAAGAAG 
CCACGTCCCA CTCGCGCTGA GATCTCTGAT GTGGCCAACG CTGTGCTCGA TGGTGCTGAT 
TGCGTCATGT TGTCTGGTGA GACCGCCAAG GGCGAGTACC CGCTGGAGTG CGTCCTGACC 
ATGGCCAAGA CCTGCAAGGA GGCCGAGGCT GCCCTCTGGC ACCAGAACCT CTTTAACGAC 
TTGGTTCGCG GCGCTGGTAC CATCGATGCC TCTCACGCGG CTGCCATCGC TGCCGTTGAG 
GCTGCCACCA AGGCCAAGGC CTCCGCCATC GTGGTGATCA CCACCAGCGG CAAGTCGGCC 
TTCCAGGTGA GCAAGTACCG CCCACGCTGC CCCATCATCG CGGTCACCCG TTTCGCGCAG 
ACCGCCCGAC AGGCCCATCT CTACCGTGGA CTGGTGCCAC TCATCTACAA GGAGCCCGGT 
CTTGGTGACT GGCTGAAGGA CGTGGACGTG CGCGTGCAGT TCGGTCTGCA GGTCGGAAAG 
AAGAACGGCT TCATCAAGAC CGGCGATTCC GTCGTGGTGG TTACCGGCTG GAAGCAGGGC 
TCCGGCTTCA CCAACACCAT CCGCATTGTC ACCGTCGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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PyK cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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