GatA cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	GatA
Entrez Gene ID	42283
Full Name	Glutamyl-tRNA amidotransferase, subunit A
Synonyms	0929/02,CG6007,Dmel\CG6007,bene,benedict,gatA,l(3)S092902
General protein information	Preferred Names Glutamyl-tRNA amidotransferase, subunit A Names CG6007 gene product from transcript CG6007-RA CG6007-PA GatA-PA Glutamyl-tRNA(Gln) amidotransferase, subunit A benedict gatA glutamyl-tRNA amidotransferase A glutamyl-tRNA amidotransferase subunit A lethal (3) S092902
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 91E4-91E4

mRNA and Protein(s)

mRNA	Protein	Name
NM_142518.4	NP_650775.2	Glutamyl-tRNA amidotransferase, subunit A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	QRSL1	XP_518663.2
Canis lupus familiaris (dog)	QRSL1	XP_532250.3
Mus musculus (house mouse)	Qrsl1	NP_001074523.1
Rattus norvegicus (Norway rat)	Qrsl1	NP_001014056.1
Danio rerio (zebrafish)	qrsl1	NP_001112370.1
Caenorhabditis elegans (roundworm)	Y41D4A.6	NP_500129.1
Saccharomyces cerevisiae (baker's yeast)	HER2	NP_014021.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004057	XP_309611.3
Xenopus tropicalis (tropical clawed frog)	qrsl1	NP_001072779.1
Kluyveromyces lactis	KLLA0A09845g	XP_451430.1
Neurospora crassa	NCU00660	XP_965800.1
Magnaporthe oryzae (rice blast fungus)	MGG_13483	XP_003716212.1
Macaca mulatta (Rhesus monkey)	QRSL1	XP_001089760.1
Drosophila melanogaster (fruit fly)	gatA	NP_650775.2
Homo sapiens (human)	QRSL1	NP_060762.3
Bos taurus (cattle)	QRSL1	NP_001039801.1
Gallus gallus (chicken)	QRSL1	XP_419807.2
Arabidopsis thaliana (thale cress)	AT3G25660	NP_189194.1
Eremothecium gossypii	AGOS_ABR140C	NP_983087.2
Schizosaccharomyces pombe (fission yeast)	SPBC646.03	NP_595361.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GatA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GatA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05366	NM_142518.4 Latest version!	Drosophila melanogaster Glutamyl-tRNA amidotransferase, subunit A (GatA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GatA ( NM_142518.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650775.2 Drosophila melanogaster Glutamyl-tRNA amidotransferase, subunit A (GatA), mRNA.

Price & Availability↑

CloneID	OFa05366
Clone ID Related Accession (Same CDS sequence)	NM_142518.4
Accession Version	NM_142518.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Glutamyl-tRNA amidotransferase, subunit A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_142518.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGGCGTC ACTTACAGTG GAGCATTAAG CAGCTAACAG CGAGTTACTC AGATGGTCAG 
CTGTCTCCTC GCCGGGTGAC CGAAGATGCT CTGCAAGATG CCCTGCGGTG GAAGACACTG 
AATGCCTTTG TGCGCCTCAC GCCGGAACAG GCGGGGCAGC AGGCCCAGGA CGCGGAGCAG 
CGCTATAAGC TAAGGCAACC GATTAGTGAT CTTGATGGAG TCACCATTGC CATCAAGGAC 
AACTTTTGCA CCAAAGATGT ACACACCACC TGTGCCTCAC GGATGCTGCA AGACTTTGTG 
CCTCCCTATG ATGCCACTGT GTGCTCCAGA CTTAAACAAG CCGGCGCTGT AATTCTGGGC 
AAGACGAATA TGGATCAGTT TGCCATGGGC GCCGGCACTG TGGACTCCCT GTACGGGCCG 
ACTAAGAACA TCTGGAGCGA GGACCTTAAC AAGGATCACT GGCGCATCGC GGGCGGCAGT 
TCTGGAGGCT CTGCATCCGC CGTAGCCGCG GGTCTTTGTT ATGCAGCAAT TGGTTCGGAC 
ACGGGTGGCT CCACCCGAAA CCCGGCTTCG TACTGCGGCG TGGTGGGTCT AAAGCCCACC 
TACGGCCTCG TCTCCCGCCA CGGTCTTATT CCGCTGGTCA ATTCCATGGA CGTGCCCGGC 
ATTTTTGCCC GCTCCGTCAG CGATTGCGTG GCGGTACTGA ATACAGTGGC AGGGCCGGAT 
AAACTGGACT CCACAACCAT CAGGCAGCCT TTTACCAGGT TACATCTGCC GGAAGTTGGG 
CAAATTGACC TGAGCACTGT GCGCATAGGA ATACCAAAGG AATACCATTG CCACGGTCTT 
TCCGCCGAAG TTCTGGAAAC GTGGTCTAAG GTGGCCGATT TGCTGGAGTG TAGCGGTGCC 
TCCGTCAGAC AGGTTTCCCT GCCCAATACG GCAGCAAGTA TATTCGTGTA CACCATTCTT 
AATCAGTGCG AGGTGGCCAG CAACATGGCA AGGTACGATG GAATCGAGTA CGGCCATCGG 
GCAACAGACG AGCGCAGCAC GGAGCAATTG TATGCCCTAT CCAGGGCCGA GGGATTCAAC 
GATGTGGTTA AGACACGCAT ACTCACTGGA AACTTTCTGC TGCTGAAAAA GAACTATGAT 
CACTACTTTG AGAAGGCTTT GCGGGTGCGC CGCCTTATTG CAGAGGACTT TGCAAGGGTG 
TTTGATTCCT CGGCCAAAGA GGAGCGCGTG GACATCCTTC TCACGCCGAC AACTCTTACA 
GAGGCTCCGC TGTACAAGGA CTTCGCCTCC CTGACCAACA GGGATCAGTG TGCCGTGCAG 
GACTTTTGCA CTCAGCCAGC CAACATGGCT GGAATTCCCG CCGTGTCTAT ACCGATTCGC 
CTGTCCCAGG CCGGATTGCC CTTGAGCCTT CAGCTGATGA GCAACAGCCT CAACGAGCAG 
TTACTGCTCA CAGTGGCACG ATGGATAGAG GCGCAGGTGG AATTCGACAG CCTAGAGCAC 
TCGCAACAGT ATAAGGCCAG TTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650775.2
CDS	107..1633
Translation	MRRHLQWSIKQLTASYSDGQLSPRRVTEDALQDALRWKTLNAFVRLTPEQAGQQAQDAEQRYKLRQPISDLDGVTIAIKDNFCTKDVHTTCASRMLQDFVPPYDATVCSRLKQAGAVILGKTNMDQFAMGAGTVDSLYGPTKNIWSEDLNKDHWRIAGGSSGGSASAVAAGLCYAAIGSDTGGSTRNPASYCGVVGLKPTYGLVSRHGLIPLVNSMDVPGIFARSVSDCVAVLNTVAGPDKLDSTTIRQPFTRLHLPEVGQIDLSTVRIGIPKEYHCHGLSAEVLETWSKVADLLECSGASVRQVSLPNTAASIFVYTILNQCEVASNMARYDGIEYGHRATDERSTEQLYALSRAEGFNDVVKTRILTGNFLLLKKNYDHYFEKALRVRRLIAEDFARVFDSSAKEERVDILLTPTTLTEAPLYKDFASLTNRDQCAVQDFCTQPANMAGIPAVSIPIRLSQAGLPLSLQLMSNSLNEQLLLTVARWIEAQVEFDSLEHSQQYKASL

Target ORF information:

RefSeq Version	NM_142518.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Glutamyl-tRNA amidotransferase, subunit A (GatA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142518.4

ORF Insert Sequence:

ATGCGGCGTC ACTTACAGTG GAGCATTAAG CAGCTAACAG CGAGTTACTC AGATGGTCAG 
CTGTCTCCTC GCCGGGTGAC CGAAGATGCT CTGCAAGATG CCCTGCGGTG GAAGACACTG 
AATGCCTTTG TGCGCCTCAC GCCGGAACAG GCGGGGCAGC AGGCCCAGGA CGCGGAGCAG 
CGCTATAAGC TAAGGCAACC GATTAGTGAT CTTGATGGAG TCACCATTGC CATCAAGGAC 
AACTTTTGCA CCAAAGATGT ACACACCACC TGTGCCTCAC GGATGCTGCA AGACTTTGTG 
CCTCCCTATG ATGCCACTGT GTGCTCCAGA CTTAAACAAG CCGGCGCTGT AATTCTGGGC 
AAGACGAATA TGGATCAGTT TGCCATGGGC GCCGGCACTG TGGACTCCCT GTACGGGCCG 
ACTAAGAACA TCTGGAGCGA GGACCTTAAC AAGGATCACT GGCGCATCGC GGGCGGCAGT 
TCTGGAGGCT CTGCATCCGC CGTAGCCGCG GGTCTTTGTT ATGCAGCAAT TGGTTCGGAC 
ACGGGTGGCT CCACCCGAAA CCCGGCTTCG TACTGCGGCG TGGTGGGTCT AAAGCCCACC 
TACGGCCTCG TCTCCCGCCA CGGTCTTATT CCGCTGGTCA ATTCCATGGA CGTGCCCGGC 
ATTTTTGCCC GCTCCGTCAG CGATTGCGTG GCGGTACTGA ATACAGTGGC AGGGCCGGAT 
AAACTGGACT CCACAACCAT CAGGCAGCCT TTTACCAGGT TACATCTGCC GGAAGTTGGG 
CAAATTGACC TGAGCACTGT GCGCATAGGA ATACCAAAGG AATACCATTG CCACGGTCTT 
TCCGCCGAAG TTCTGGAAAC GTGGTCTAAG GTGGCCGATT TGCTGGAGTG TAGCGGTGCC 
TCCGTCAGAC AGGTTTCCCT GCCCAATACG GCAGCAAGTA TATTCGTGTA CACCATTCTT 
AATCAGTGCG AGGTGGCCAG CAACATGGCA AGGTACGATG GAATCGAGTA CGGCCATCGG 
GCAACAGACG AGCGCAGCAC GGAGCAATTG TATGCCCTAT CCAGGGCCGA GGGATTCAAC 
GATGTGGTTA AGACACGCAT ACTCACTGGA AACTTTCTGC TGCTGAAAAA GAACTATGAT 
CACTACTTTG AGAAGGCTTT GCGGGTGCGC CGCCTTATTG CAGAGGACTT TGCAAGGGTG 
TTTGATTCCT CGGCCAAAGA GGAGCGCGTG GACATCCTTC TCACGCCGAC AACTCTTACA 
GAGGCTCCGC TGTACAAGGA CTTCGCCTCC CTGACCAACA GGGATCAGTG TGCCGTGCAG 
GACTTTTGCA CTCAGCCAGC CAACATGGCT GGAATTCCCG CCGTGTCTAT ACCGATTCGC 
CTGTCCCAGG CCGGATTGCC CTTGAGCCTT CAGCTGATGA GCAACAGCCT CAACGAGCAG 
TTACTGCTCA CAGTGGCACG ATGGATAGAG GCGCAGGTGG AATTCGACAG CCTAGAGCAC 
TCGCAACAGT ATAAGGCCAG TTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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GatA cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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