CstF64 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CstF64
Entrez Gene ID	42239
Full Name	Cleavage stimulation factor 64 kilodalton subunit
Synonyms	CG7697,CStF 64,CstF 64,CstF64,Dmel\CG7697,dCstF64
General protein information	Preferred Names Cleavage stimulation factor 64 kilodalton subunit Names CG7697 gene product from transcript CG7697-RA CG7697-PA CstF-64-PA cleavage stimulation factor cleavage stimulation factor 64 kilodalton subunit
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 91B8-91B8

mRNA and Protein(s)

mRNA	Protein	Name
NM_058105.5	NP_477453.1	cleavage stimulation factor 64 kD subunit

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CstF-64	NP_477453.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010918	XP_309774.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CstF64 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CstF64 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05327	NM_058105.5 Latest version!	Drosophila melanogaster cleavage stimulation factor 64 kD subunit (CstF64), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CstF64 ( NM_058105.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477453.1 Drosophila melanogaster cleavage stimulation factor 64 kD subunit (CstF64), mRNA.

Price & Availability↑

CloneID	OFa05327
Clone ID Related Accession (Same CDS sequence)	NM_058105.5
Accession Version	NM_058105.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	cleavage stimulation factor 64 kD subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_058105.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGATA AGGCGCAGGA ACAAAGTATA ATGGACAAGT CCATGCGGTC GGTTTTTGTG 
GGGAACATAC CCTACGAGGC CACCGAGGAG AAGCTAAAGG AGATTTTCAG CGAAGTGGGC 
CCGGTCTTGT CACTGAAACT GGTCTTTGAT CGGGAAAGTG GCAAACCCAA GGGCTTTGGC 
TTCTGCGAGT ACAAGGACCA GGAGACGGCT TTAAGTGCCA TGCGGAATCT GAATGGCTAT 
GAGATCGGCG GCAGAACACT GCGAGTGGAC AACGCGTGTA CGGAGAAGTC GCGCATGGAG 
ATGCAGCAGC TGCTTCAGGG ACCCCAGGTG GAGAATCCCT ATGGCGAGCC CTGCGAACCA 
GAGGATGCTC CCGAGCTCAT CACCAAAACG GTGGCTTCCT TGCCGCCGGA GCAAATGTAC 
GAGCTGATGA AGCAGATGAA GCTCTGCATC GTGAGCAATC CATCGGAGGC ACGCCAGATG 
CTAATGCTCA ATCCTCAGTT GGCGTACGCT TTGCTCCAGG CCATGGTGGT TATGAGGATC 
GTGGACCCTC AGCAGGCGCT CGGGATGCTC TTCAAGGCCA ACCAAATGCC CCCCGTCTTG 
GGCGGCAATC CGCACCAGGG GCCGGGGAAT CACACCATGA TGGGTCAGCA ACAGGTTCCG 
CAACAGCAGG TGCAGATCCC GCAGCAACAG CAACAGGCTC CCCAGCCACC AATGCCCGTT 
CCCGGACCTG GCTTCCCGGC CAATGTCCAT CCGAACGATA TCGATTTGCG CATGGTGCCG 
GGTGGACCCA TGCCGATGGA TCCCAGGATG ATGGGCCGTG GCATGGACCA GGATTTGCGA 
GCTTCTCTAC CCAATCCAGT GCCACCGCCG CTGATGGATC CCCGAGCTCG TGCCCAGATG 
CCCCCACAGC AGCAGCAGGG CGTTCCGCAG GCGCCACCGG CCCCCTATCC CAGCGATCCG 
CGCCAGCGTC CCATGGATCC CAGGTTAAGG GCAGGACCTG GTCCTCAGCA GCAGGCGCCG 
CCACAAGGAA TCCCCCAAGC GCCGCCACCG ACACAACAAC AGCAGGCGGC CGCTCAGCAG 
TTGCAGAGCA GACTAGGCGC CCATGGAGTC CTGCCCTCGG ATGCCTCCGA CCAGGAGAAG 
GCTGCCCTCA TCATGCAGGT ACTGCAGCTG TCCGACGAAC AGATTGCGCA GCTGCCCTCC 
GAGCAACGGG TCAGTATTGT CATGCTCAAA GAGCAGATTG CGAAGAGTAC CCAGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477453.1
CDS	88..1347
Translation	MADKAQEQSIMDKSMRSVFVGNIPYEATEEKLKEIFSEVGPVLSLKLVFDRESGKPKGFGFCEYKDQETALSAMRNLNGYEIGGRTLRVDNACTEKSRMEMQQLLQGPQVENPYGEPCEPEDAPELITKTVASLPPEQMYELMKQMKLCIVSNPSEARQMLMLNPQLAYALLQAMVVMRIVDPQQALGMLFKANQMPPVLGGNPHQGPGNHTMMGQQQVPQQQVQIPQQQQQAPQPPMPVPGPGFPANVHPNDIDLRMVPGGPMPMDPRMMGRGMDQDLRASLPNPVPPPLMDPRARAQMPPQQQQGVPQAPPAPYPSDPRQRPMDPRLRAGPGPQQQAPPQGIPQAPPPTQQQQAAAQQLQSRLGAHGVLPSDASDQEKAALIMQVLQLSDEQIAQLPSEQRVSIVMLKEQIAKSTQR

Target ORF information:

RefSeq Version	NM_058105.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cleavage stimulation factor 64 kD subunit (CstF64), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058105.5

ORF Insert Sequence:

ATGGCAGATA AGGCGCAGGA ACAAAGTATA ATGGACAAGT CCATGCGGTC GGTTTTTGTG 
GGGAACATAC CCTACGAGGC CACCGAGGAG AAGCTAAAGG AGATTTTCAG CGAAGTGGGC 
CCGGTCTTGT CACTGAAACT GGTCTTTGAT CGGGAAAGTG GCAAACCCAA GGGCTTTGGC 
TTCTGCGAGT ACAAGGACCA GGAGACGGCT TTAAGTGCCA TGCGGAATCT GAATGGCTAT 
GAGATCGGCG GCAGAACACT GCGAGTGGAC AACGCGTGTA CGGAGAAGTC GCGCATGGAG 
ATGCAGCAGC TGCTTCAGGG ACCCCAGGTG GAGAATCCCT ATGGCGAGCC CTGCGAACCA 
GAGGATGCTC CCGAGCTCAT CACCAAAACG GTGGCTTCCT TGCCGCCGGA GCAAATGTAC 
GAGCTGATGA AGCAGATGAA GCTCTGCATC GTGAGCAATC CATCGGAGGC ACGCCAGATG 
CTAATGCTCA ATCCTCAGTT GGCGTACGCT TTGCTCCAGG CCATGGTGGT TATGAGGATC 
GTGGACCCTC AGCAGGCGCT CGGGATGCTC TTCAAGGCCA ACCAAATGCC CCCCGTCTTG 
GGCGGCAATC CGCACCAGGG GCCGGGGAAT CACACCATGA TGGGTCAGCA ACAGGTTCCG 
CAACAGCAGG TGCAGATCCC GCAGCAACAG CAACAGGCTC CCCAGCCACC AATGCCCGTT 
CCCGGACCTG GCTTCCCGGC CAATGTCCAT CCGAACGATA TCGATTTGCG CATGGTGCCG 
GGTGGACCCA TGCCGATGGA TCCCAGGATG ATGGGCCGTG GCATGGACCA GGATTTGCGA 
GCTTCTCTAC CCAATCCAGT GCCACCGCCG CTGATGGATC CCCGAGCTCG TGCCCAGATG 
CCCCCACAGC AGCAGCAGGG CGTTCCGCAG GCGCCACCGG CCCCCTATCC CAGCGATCCG 
CGCCAGCGTC CCATGGATCC CAGGTTAAGG GCAGGACCTG GTCCTCAGCA GCAGGCGCCG 
CCACAAGGAA TCCCCCAAGC GCCGCCACCG ACACAACAAC AGCAGGCGGC CGCTCAGCAG 
TTGCAGAGCA GACTAGGCGC CCATGGAGTC CTGCCCTCGG ATGCCTCCGA CCAGGAGAAG 
GCTGCCCTCA TCATGCAGGT ACTGCAGCTG TCCGACGAAC AGATTGCGCA GCTGCCCTCC 
GAGCAACGGG TCAGTATTGT CATGCTCAAA GAGCAGATTG CGAAGAGTAC CCAGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CstF64 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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