Vha100-2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vha100-2
Entrez Gene ID	42216
Full Name	Vacuolar H[+] ATPase 100kD subunit 2
Synonyms	BcDNA:LD21735,CG18617,CG7679,Dmel\CG18617,anon-WO0118547.296,vha100-2
General protein information	Preferred Names Vacuolar H[+] ATPase 100kD subunit 2 Names CG18617 gene product from transcript CG18617-RB CG18617-PA CG18617-PB Vha100-2-PA Vha100-2-PB vacuolar H + ATPase subunit 100-2 vacuolar H[+] ATPase 100kD subunit 2 vacuolar H[+] ATPase subunit 100-2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 91A5-91A5

mRNA and Protein(s)

mRNA	Protein	Name
NM_169810.2	NP_732337.1	vacuolar H[+] ATPase 100kD subunit 2, isoform A
NM_142465.2	NP_650722.1	vacuolar H[+] ATPase 100kD subunit 2, isoform B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ATP6V0A4	XP_539895.2
Rattus norvegicus (Norway rat)	Atp6v0a4	NP_001100061.1
Pan troglodytes (chimpanzee)	ATP6V0A4	XP_003318871.1
Bos taurus (cattle)	ATP6V0A4	NP_001192780.1
Mus musculus (house mouse)	Atp6v0a4	NP_536715.3
Xenopus tropicalis (tropical clawed frog)	atp6v0a4	NP_001107162.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001587	XP_321521.2
Gallus gallus (chicken)	ATP6V0A4	NP_001073571.2
Arabidopsis thaliana (thale cress)	VHA-A3	NP_568051.1
Arabidopsis thaliana (thale cress)	VHA-A2	NP_179736.1
Oryza sativa (rice)	Os03g0251500	NP_001049572.1
Homo sapiens (human)	ATP6V0A4	NP_570856.2
Macaca mulatta (Rhesus monkey)	ATP6V0A4	XP_002803521.1
Drosophila melanogaster (fruit fly)	Vha100-2	NP_650722.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Vha100-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vha100-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05303	NM_142465.2 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 2, transcript variant B (Vha100-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OFa05303	NM_169810.2 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 2, transcript variant A (Vha100-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vha100-2 ( NM_169810.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732337.1 Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 2, transcript variant A (Vha100-2), mRNA.

Price & Availability↑

CloneID	OFa05303
Clone ID Related Accession (Same CDS sequence)	NM_169810.2 , NM_142465.2
Accession Version	NM_169810.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 100kD subunit 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169810.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGGGACA TGTTCCGTAG TGAGGAGATG GCACTCTGCC AGATGTTCAT TCAGCCGGAG 
GCCGCGTATA CCTCCGTATC TGAGCTGGGC GAAACCGGCT GCGTGCAGTT CCGCGACTTG 
AATGTGAACG TGAACGCCTT CCAGCGCAAG TTCGTCACCG AGGTGCGTCG CTGCGATGAG 
CTGGAGCGCA AGATCCGCTA CATCGAGACG GAGATCAAGA AGGACGGCAT CGTCCTGCCC 
GACATCCAGG ATGACATTCC GCGTGCGCCC AATCCACGCG AGATCATCGA TCTGGAGGCG 
CATCTGGAGA AGACCGAGTC GGAGATGATC GAGCTGGCCC AGAACGAGGT GAACATGAAG 
TCCAACTATC TGGAGCTGAC CGAGCTGCGC AAGGTGCTGG AGAACACGCA GGGCTTCTTC 
TCCGACCAGG AGGTTCTCAA TCTGGACTCC TCCAACCGAG CTGGAGGAGA CAACGATGCT 
GCTGCTCAAC ACCGTGGCCG GCTTGGATTC GTTGCCGGTG TAATTAACCG GGAGCGAGTG 
TTTGCCTTTG AGCGTATGCT GTGGCGCATC TCCAGGGGCA ATGTCTTCCT CAAGCGCTCC 
GATCTGGACG AGCCGCTGAA CGATCCGGCC ACCGGACATC CCATCTACAA GACCGTCTTC 
GTGGCCTTCT TCCAGGGCGA GCAACTGAAG AACCGTATCA AGAAGGTGTG CACTGGCTTC 
CACGCCTCGC TGTATCCCTG TCCCAGCTCG CACAACGAGC GCGAGGAAAT GGTTCGCAAT 
GTGCGCACCC GCCTGGAGGA TCTGAAGCTG GTCCTTAGCC AGACGGAGGA TCATCGTAGC 
CGCGTCCTGG CCACCGTGTC CAAGAATCTG CCCTCGTGGT CGATCATGGT CAAGAAGATG 
AAGGCCATTT ACCACACGCT GAATCTGTTC AACATGGACG TGACCAAGAA GTGCCTGATT 
GGCGAGTGTT GGGTGCCCAC CAATGATTTG CCCGTTGTCC AAAAGGCTCT GTCCGATGGA 
TCTGCTGCAG TGGGCAGCAC CATACCCTCG TTCCTGAACG TGATCGACAC CAACGAGCAG 
CCGCCGACCT TTAACAGGAC TAACAAGTTT ACCCGTGGCT TCCAGAATCT GATTGATGCC 
TACGGAGTGG CCTCGTACAG AGAGTGCAAT CCCGCCCTGT ACACCTGCAT CACCTTCCCC 
TTCCTTTTCG CTGTGATGTT CGGCGATTTG GGTCACGGCC TTATTCTGGT TTTGTTTGGA 
GCTTGGATGG TTTTGTGCGA GCGCAAGCTG GCTCGCATCC GCAACGGTGG TGAGATCTGG 
AACATCTTCT TCGGCGGTCG CTATATCATT CTGCTGATGG GTCTGTTTGC CATGTACACT 
GGTTTGGTTT ACAACGATGT CTTCTCCAAG TCGATGAACC TGTTTGGATC ACGTTGGTTC 
AACAACTACA ACACAACGAC TGTCCTGACC AACCCGAATC TGCAGTTGCC GCCCAACAGC 
TCCGCCGTGG GTGTCTATCC CTTCGGAATG GATCCCGTGT GGCAGTTGGC TGATAACAAG 
ATCATCTTCC TCAACAGTTT CAAGATGAAG CTCTCGATCA TCTTCGGAGT GCTGCACATG 
GTCTTCGGCG TGTGCATGTC GGTCGTTAAC TTCACCCACT TCAAGCGTTA TGCCTCCATT 
TTCCTGGAGT TCGTGCCCCA AATTCTGTTC CTGCTACTGC TCTTCGGCTA CATGGTGTTC 
ATGATGTTCT TCAAGTGGTT CAGCTATAAC GCTAGGACTA GCTTCCAGCC AGAAACTCCT 
GGATGCGCTC CCTCCGTGTT GATCATGTTC ATCAACATGA TGCTGTTCAA GAACACTGAG 
CCACCAAAGG GTTGCAACGA GTTCATGTTC GAGTCACAGC CCCAGTTGCA GAAGGCCTTT 
GTGCTCATCG CCCTGTGCTG CATTCCTTGG ATGCTTCTGG GCAAGCCCCT GTACATCAAG 
TTCACTCGCA AAAACAAGGC TCATGCCAAT CACAATGGTC AGTTGACCGG CAACATTGAA 
CTGGCCGAAG GCGAGACTCC TCTGCCCACA GGATTCTCTG GAAACGAGGA GAATGCCGGG 
GGTGCCCATG GCCATGACGA TGAGCCCATG AGCGAAATCT ACATCCATCA AGCCATCCAC 
ACCATCGAAT ATGTGCTCAG TACCATCTCG CACACGGCGT CCTATCTGCG TCTCTGGGCT 
CTGTCCCTGG CTCACGCCCA GCTCTCCGAG GTGCTGTGGC AAATGGTGTT GTCCCTGGGC 
CTTAAGATGT CCGGCGTGGG CGGTGCCATT GGTCTGTTCA TCATCTTCGG CGCCTGGTGC 
TTGTTCACCC TGGCTATCCT GGTCCTCATG GAGGGTCTGT CCGCCTTCCT GCACACTCTG 
CGTCTGCACT GGGTGGAGTT CATGAGCAAG TTCTACGAGG GAATGGGCTA CGCCTTCCAG 
CCGTTCAGCT TCAAGGCCAT TCTCGATGGC GAAGAGGAGG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732337.1
CDS	238..2742
Translation	MGDMFRSEEMALCQMFIQPEAAYTSVSELGETGCVQFRDLNVNVNAFQRKFVTEVRRCDELERKIRYIETEIKKDGIVLPDIQDDIPRAPNPREIIDLEAHLEKTESEMIELAQNEVNMKSNYLELTELRKVLENTQGFFSDQEVLNLDSSNRAGGDNDAAAQHRGRLGFVAGVINRERVFAFERMLWRISRGNVFLKRSDLDEPLNDPATGHPIYKTVFVAFFQGEQLKNRIKKVCTGFHASLYPCPSSHNEREEMVRNVRTRLEDLKLVLSQTEDHRSRVLATVSKNLPSWSIMVKKMKAIYHTLNLFNMDVTKKCLIGECWVPTNDLPVVQKALSDGSAAVGSTIPSFLNVIDTNEQPPTFNRTNKFTRGFQNLIDAYGVASYRECNPALYTCITFPFLFAVMFGDLGHGLILVLFGAWMVLCERKLARIRNGGEIWNIFFGGRYIILLMGLFAMYTGLVYNDVFSKSMNLFGSRWFNNYNTTTVLTNPNLQLPPNSSAVGVYPFGMDPVWQLADNKIIFLNSFKMKLSIIFGVLHMVFGVCMSVVNFTHFKRYASIFLEFVPQILFLLLLFGYMVFMMFFKWFSYNARTSFQPETPGCAPSVLIMFINMMLFKNTEPPKGCNEFMFESQPQLQKAFVLIALCCIPWMLLGKPLYIKFTRKNKAHANHNGQLTGNIELAEGETPLPTGFSGNEENAGGAHGHDDEPMSEIYIHQAIHTIEYVLSTISHTASYLRLWALSLAHAQLSEVLWQMVLSLGLKMSGVGGAIGLFIIFGAWCLFTLAILVLMEGLSAFLHTLRLHWVEFMSKFYEGMGYAFQPFSFKAILDGEEEE

Target ORF information:

RefSeq Version	NM_169810.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 2, transcript variant A (Vha100-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169810.2

ORF Insert Sequence:

ATGGGGGACA TGTTCCGTAG TGAGGAGATG GCACTCTGCC AGATGTTCAT TCAGCCGGAG 
GCCGCGTATA CCTCCGTATC TGAGCTGGGC GAAACCGGCT GCGTGCAGTT CCGCGACTTG 
AATGTGAACG TGAACGCCTT CCAGCGCAAG TTCGTCACCG AGGTGCGTCG CTGCGATGAG 
CTGGAGCGCA AGATCCGCTA CATCGAGACG GAGATCAAGA AGGACGGCAT CGTCCTGCCC 
GACATCCAGG ATGACATTCC GCGTGCGCCC AATCCACGCG AGATCATCGA TCTGGAGGCG 
CATCTGGAGA AGACCGAGTC GGAGATGATC GAGCTGGCCC AGAACGAGGT GAACATGAAG 
TCCAACTATC TGGAGCTGAC CGAGCTGCGC AAGGTGCTGG AGAACACGCA GGGCTTCTTC 
TCCGACCAGG AGGTTCTCAA TCTGGACTCC TCCAACCGAG CTGGAGGAGA CAACGATGCT 
GCTGCTCAAC ACCGTGGCCG GCTTGGATTC GTTGCCGGTG TAATTAACCG GGAGCGAGTG 
TTTGCCTTTG AGCGTATGCT GTGGCGCATC TCCAGGGGCA ATGTCTTCCT CAAGCGCTCC 
GATCTGGACG AGCCGCTGAA CGATCCGGCC ACCGGACATC CCATCTACAA GACCGTCTTC 
GTGGCCTTCT TCCAGGGCGA GCAACTGAAG AACCGTATCA AGAAGGTGTG CACTGGCTTC 
CACGCCTCGC TGTATCCCTG TCCCAGCTCG CACAACGAGC GCGAGGAAAT GGTTCGCAAT 
GTGCGCACCC GCCTGGAGGA TCTGAAGCTG GTCCTTAGCC AGACGGAGGA TCATCGTAGC 
CGCGTCCTGG CCACCGTGTC CAAGAATCTG CCCTCGTGGT CGATCATGGT CAAGAAGATG 
AAGGCCATTT ACCACACGCT GAATCTGTTC AACATGGACG TGACCAAGAA GTGCCTGATT 
GGCGAGTGTT GGGTGCCCAC CAATGATTTG CCCGTTGTCC AAAAGGCTCT GTCCGATGGA 
TCTGCTGCAG TGGGCAGCAC CATACCCTCG TTCCTGAACG TGATCGACAC CAACGAGCAG 
CCGCCGACCT TTAACAGGAC TAACAAGTTT ACCCGTGGCT TCCAGAATCT GATTGATGCC 
TACGGAGTGG CCTCGTACAG AGAGTGCAAT CCCGCCCTGT ACACCTGCAT CACCTTCCCC 
TTCCTTTTCG CTGTGATGTT CGGCGATTTG GGTCACGGCC TTATTCTGGT TTTGTTTGGA 
GCTTGGATGG TTTTGTGCGA GCGCAAGCTG GCTCGCATCC GCAACGGTGG TGAGATCTGG 
AACATCTTCT TCGGCGGTCG CTATATCATT CTGCTGATGG GTCTGTTTGC CATGTACACT 
GGTTTGGTTT ACAACGATGT CTTCTCCAAG TCGATGAACC TGTTTGGATC ACGTTGGTTC 
AACAACTACA ACACAACGAC TGTCCTGACC AACCCGAATC TGCAGTTGCC GCCCAACAGC 
TCCGCCGTGG GTGTCTATCC CTTCGGAATG GATCCCGTGT GGCAGTTGGC TGATAACAAG 
ATCATCTTCC TCAACAGTTT CAAGATGAAG CTCTCGATCA TCTTCGGAGT GCTGCACATG 
GTCTTCGGCG TGTGCATGTC GGTCGTTAAC TTCACCCACT TCAAGCGTTA TGCCTCCATT 
TTCCTGGAGT TCGTGCCCCA AATTCTGTTC CTGCTACTGC TCTTCGGCTA CATGGTGTTC 
ATGATGTTCT TCAAGTGGTT CAGCTATAAC GCTAGGACTA GCTTCCAGCC AGAAACTCCT 
GGATGCGCTC CCTCCGTGTT GATCATGTTC ATCAACATGA TGCTGTTCAA GAACACTGAG 
CCACCAAAGG GTTGCAACGA GTTCATGTTC GAGTCACAGC CCCAGTTGCA GAAGGCCTTT 
GTGCTCATCG CCCTGTGCTG CATTCCTTGG ATGCTTCTGG GCAAGCCCCT GTACATCAAG 
TTCACTCGCA AAAACAAGGC TCATGCCAAT CACAATGGTC AGTTGACCGG CAACATTGAA 
CTGGCCGAAG GCGAGACTCC TCTGCCCACA GGATTCTCTG GAAACGAGGA GAATGCCGGG 
GGTGCCCATG GCCATGACGA TGAGCCCATG AGCGAAATCT ACATCCATCA AGCCATCCAC 
ACCATCGAAT ATGTGCTCAG TACCATCTCG CACACGGCGT CCTATCTGCG TCTCTGGGCT 
CTGTCCCTGG CTCACGCCCA GCTCTCCGAG GTGCTGTGGC AAATGGTGTT GTCCCTGGGC 
CTTAAGATGT CCGGCGTGGG CGGTGCCATT GGTCTGTTCA TCATCTTCGG CGCCTGGTGC 
TTGTTCACCC TGGCTATCCT GGTCCTCATG GAGGGTCTGT CCGCCTTCCT GCACACTCTG 
CGTCTGCACT GGGTGGAGTT CATGAGCAAG TTCTACGAGG GAATGGGCTA CGCCTTCCAG 
CCGTTCAGCT TCAAGGCCAT TCTCGATGGC GAAGAGGAGG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vha100-2 ( NM_142465.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650722.1 Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 2, transcript variant B (Vha100-2), mRNA.

Price & Availability↑

CloneID	OFa05303
Clone ID Related Accession (Same CDS sequence)	NM_169810.2 , NM_142465.2
Accession Version	NM_142465.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 100kD subunit 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142465.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGGGACA TGTTCCGTAG TGAGGAGATG GCACTCTGCC AGATGTTCAT TCAGCCGGAG 
GCCGCGTATA CCTCCGTATC TGAGCTGGGC GAAACCGGCT GCGTGCAGTT CCGCGACTTG 
AATGTGAACG TGAACGCCTT CCAGCGCAAG TTCGTCACCG AGGTGCGTCG CTGCGATGAG 
CTGGAGCGCA AGATCCGCTA CATCGAGACG GAGATCAAGA AGGACGGCAT CGTCCTGCCC 
GACATCCAGG ATGACATTCC GCGTGCGCCC AATCCACGCG AGATCATCGA TCTGGAGGCG 
CATCTGGAGA AGACCGAGTC GGAGATGATC GAGCTGGCCC AGAACGAGGT GAACATGAAG 
TCCAACTATC TGGAGCTGAC CGAGCTGCGC AAGGTGCTGG AGAACACGCA GGGCTTCTTC 
TCCGACCAGG AGGTTCTCAA TCTGGACTCC TCCAACCGAG CTGGAGGAGA CAACGATGCT 
GCTGCTCAAC ACCGTGGCCG GCTTGGATTC GTTGCCGGTG TAATTAACCG GGAGCGAGTG 
TTTGCCTTTG AGCGTATGCT GTGGCGCATC TCCAGGGGCA ATGTCTTCCT CAAGCGCTCC 
GATCTGGACG AGCCGCTGAA CGATCCGGCC ACCGGACATC CCATCTACAA GACCGTCTTC 
GTGGCCTTCT TCCAGGGCGA GCAACTGAAG AACCGTATCA AGAAGGTGTG CACTGGCTTC 
CACGCCTCGC TGTATCCCTG TCCCAGCTCG CACAACGAGC GCGAGGAAAT GGTTCGCAAT 
GTGCGCACCC GCCTGGAGGA TCTGAAGCTG GTCCTTAGCC AGACGGAGGA TCATCGTAGC 
CGCGTCCTGG CCACCGTGTC CAAGAATCTG CCCTCGTGGT CGATCATGGT CAAGAAGATG 
AAGGCCATTT ACCACACGCT GAATCTGTTC AACATGGACG TGACCAAGAA GTGCCTGATT 
GGCGAGTGTT GGGTGCCCAC CAATGATTTG CCCGTTGTCC AAAAGGCTCT GTCCGATGGA 
TCTGCTGCAG TGGGCAGCAC CATACCCTCG TTCCTGAACG TGATCGACAC CAACGAGCAG 
CCGCCGACCT TTAACAGGAC TAACAAGTTT ACCCGTGGCT TCCAGAATCT GATTGATGCC 
TACGGAGTGG CCTCGTACAG AGAGTGCAAT CCCGCCCTGT ACACCTGCAT CACCTTCCCC 
TTCCTTTTCG CTGTGATGTT CGGCGATTTG GGTCACGGCC TTATTCTGGT TTTGTTTGGA 
GCTTGGATGG TTTTGTGCGA GCGCAAGCTG GCTCGCATCC GCAACGGTGG TGAGATCTGG 
AACATCTTCT TCGGCGGTCG CTATATCATT CTGCTGATGG GTCTGTTTGC CATGTACACT 
GGTTTGGTTT ACAACGATGT CTTCTCCAAG TCGATGAACC TGTTTGGATC ACGTTGGTTC 
AACAACTACA ACACAACGAC TGTCCTGACC AACCCGAATC TGCAGTTGCC GCCCAACAGC 
TCCGCCGTGG GTGTCTATCC CTTCGGAATG GATCCCGTGT GGCAGTTGGC TGATAACAAG 
ATCATCTTCC TCAACAGTTT CAAGATGAAG CTCTCGATCA TCTTCGGAGT GCTGCACATG 
GTCTTCGGCG TGTGCATGTC GGTCGTTAAC TTCACCCACT TCAAGCGTTA TGCCTCCATT 
TTCCTGGAGT TCGTGCCCCA AATTCTGTTC CTGCTACTGC TCTTCGGCTA CATGGTGTTC 
ATGATGTTCT TCAAGTGGTT CAGCTATAAC GCTAGGACTA GCTTCCAGCC AGAAACTCCT 
GGATGCGCTC CCTCCGTGTT GATCATGTTC ATCAACATGA TGCTGTTCAA GAACACTGAG 
CCACCAAAGG GTTGCAACGA GTTCATGTTC GAGTCACAGC CCCAGTTGCA GAAGGCCTTT 
GTGCTCATCG CCCTGTGCTG CATTCCTTGG ATGCTTCTGG GCAAGCCCCT GTACATCAAG 
TTCACTCGCA AAAACAAGGC TCATGCCAAT CACAATGGTC AGTTGACCGG CAACATTGAA 
CTGGCCGAAG GCGAGACTCC TCTGCCCACA GGATTCTCTG GAAACGAGGA GAATGCCGGG 
GGTGCCCATG GCCATGACGA TGAGCCCATG AGCGAAATCT ACATCCATCA AGCCATCCAC 
ACCATCGAAT ATGTGCTCAG TACCATCTCG CACACGGCGT CCTATCTGCG TCTCTGGGCT 
CTGTCCCTGG CTCACGCCCA GCTCTCCGAG GTGCTGTGGC AAATGGTGTT GTCCCTGGGC 
CTTAAGATGT CCGGCGTGGG CGGTGCCATT GGTCTGTTCA TCATCTTCGG CGCCTGGTGC 
TTGTTCACCC TGGCTATCCT GGTCCTCATG GAGGGTCTGT CCGCCTTCCT GCACACTCTG 
CGTCTGCACT GGGTGGAGTT CATGAGCAAG TTCTACGAGG GAATGGGCTA CGCCTTCCAG 
CCGTTCAGCT TCAAGGCCAT TCTCGATGGC GAAGAGGAGG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650722.1
CDS	200..2704
Translation	MGDMFRSEEMALCQMFIQPEAAYTSVSELGETGCVQFRDLNVNVNAFQRKFVTEVRRCDELERKIRYIETEIKKDGIVLPDIQDDIPRAPNPREIIDLEAHLEKTESEMIELAQNEVNMKSNYLELTELRKVLENTQGFFSDQEVLNLDSSNRAGGDNDAAAQHRGRLGFVAGVINRERVFAFERMLWRISRGNVFLKRSDLDEPLNDPATGHPIYKTVFVAFFQGEQLKNRIKKVCTGFHASLYPCPSSHNEREEMVRNVRTRLEDLKLVLSQTEDHRSRVLATVSKNLPSWSIMVKKMKAIYHTLNLFNMDVTKKCLIGECWVPTNDLPVVQKALSDGSAAVGSTIPSFLNVIDTNEQPPTFNRTNKFTRGFQNLIDAYGVASYRECNPALYTCITFPFLFAVMFGDLGHGLILVLFGAWMVLCERKLARIRNGGEIWNIFFGGRYIILLMGLFAMYTGLVYNDVFSKSMNLFGSRWFNNYNTTTVLTNPNLQLPPNSSAVGVYPFGMDPVWQLADNKIIFLNSFKMKLSIIFGVLHMVFGVCMSVVNFTHFKRYASIFLEFVPQILFLLLLFGYMVFMMFFKWFSYNARTSFQPETPGCAPSVLIMFINMMLFKNTEPPKGCNEFMFESQPQLQKAFVLIALCCIPWMLLGKPLYIKFTRKNKAHANHNGQLTGNIELAEGETPLPTGFSGNEENAGGAHGHDDEPMSEIYIHQAIHTIEYVLSTISHTASYLRLWALSLAHAQLSEVLWQMVLSLGLKMSGVGGAIGLFIIFGAWCLFTLAILVLMEGLSAFLHTLRLHWVEFMSKFYEGMGYAFQPFSFKAILDGEEEE

Target ORF information:

RefSeq Version	NM_142465.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 2, transcript variant B (Vha100-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142465.2

ORF Insert Sequence:

ATGGGGGACA TGTTCCGTAG TGAGGAGATG GCACTCTGCC AGATGTTCAT TCAGCCGGAG 
GCCGCGTATA CCTCCGTATC TGAGCTGGGC GAAACCGGCT GCGTGCAGTT CCGCGACTTG 
AATGTGAACG TGAACGCCTT CCAGCGCAAG TTCGTCACCG AGGTGCGTCG CTGCGATGAG 
CTGGAGCGCA AGATCCGCTA CATCGAGACG GAGATCAAGA AGGACGGCAT CGTCCTGCCC 
GACATCCAGG ATGACATTCC GCGTGCGCCC AATCCACGCG AGATCATCGA TCTGGAGGCG 
CATCTGGAGA AGACCGAGTC GGAGATGATC GAGCTGGCCC AGAACGAGGT GAACATGAAG 
TCCAACTATC TGGAGCTGAC CGAGCTGCGC AAGGTGCTGG AGAACACGCA GGGCTTCTTC 
TCCGACCAGG AGGTTCTCAA TCTGGACTCC TCCAACCGAG CTGGAGGAGA CAACGATGCT 
GCTGCTCAAC ACCGTGGCCG GCTTGGATTC GTTGCCGGTG TAATTAACCG GGAGCGAGTG 
TTTGCCTTTG AGCGTATGCT GTGGCGCATC TCCAGGGGCA ATGTCTTCCT CAAGCGCTCC 
GATCTGGACG AGCCGCTGAA CGATCCGGCC ACCGGACATC CCATCTACAA GACCGTCTTC 
GTGGCCTTCT TCCAGGGCGA GCAACTGAAG AACCGTATCA AGAAGGTGTG CACTGGCTTC 
CACGCCTCGC TGTATCCCTG TCCCAGCTCG CACAACGAGC GCGAGGAAAT GGTTCGCAAT 
GTGCGCACCC GCCTGGAGGA TCTGAAGCTG GTCCTTAGCC AGACGGAGGA TCATCGTAGC 
CGCGTCCTGG CCACCGTGTC CAAGAATCTG CCCTCGTGGT CGATCATGGT CAAGAAGATG 
AAGGCCATTT ACCACACGCT GAATCTGTTC AACATGGACG TGACCAAGAA GTGCCTGATT 
GGCGAGTGTT GGGTGCCCAC CAATGATTTG CCCGTTGTCC AAAAGGCTCT GTCCGATGGA 
TCTGCTGCAG TGGGCAGCAC CATACCCTCG TTCCTGAACG TGATCGACAC CAACGAGCAG 
CCGCCGACCT TTAACAGGAC TAACAAGTTT ACCCGTGGCT TCCAGAATCT GATTGATGCC 
TACGGAGTGG CCTCGTACAG AGAGTGCAAT CCCGCCCTGT ACACCTGCAT CACCTTCCCC 
TTCCTTTTCG CTGTGATGTT CGGCGATTTG GGTCACGGCC TTATTCTGGT TTTGTTTGGA 
GCTTGGATGG TTTTGTGCGA GCGCAAGCTG GCTCGCATCC GCAACGGTGG TGAGATCTGG 
AACATCTTCT TCGGCGGTCG CTATATCATT CTGCTGATGG GTCTGTTTGC CATGTACACT 
GGTTTGGTTT ACAACGATGT CTTCTCCAAG TCGATGAACC TGTTTGGATC ACGTTGGTTC 
AACAACTACA ACACAACGAC TGTCCTGACC AACCCGAATC TGCAGTTGCC GCCCAACAGC 
TCCGCCGTGG GTGTCTATCC CTTCGGAATG GATCCCGTGT GGCAGTTGGC TGATAACAAG 
ATCATCTTCC TCAACAGTTT CAAGATGAAG CTCTCGATCA TCTTCGGAGT GCTGCACATG 
GTCTTCGGCG TGTGCATGTC GGTCGTTAAC TTCACCCACT TCAAGCGTTA TGCCTCCATT 
TTCCTGGAGT TCGTGCCCCA AATTCTGTTC CTGCTACTGC TCTTCGGCTA CATGGTGTTC 
ATGATGTTCT TCAAGTGGTT CAGCTATAAC GCTAGGACTA GCTTCCAGCC AGAAACTCCT 
GGATGCGCTC CCTCCGTGTT GATCATGTTC ATCAACATGA TGCTGTTCAA GAACACTGAG 
CCACCAAAGG GTTGCAACGA GTTCATGTTC GAGTCACAGC CCCAGTTGCA GAAGGCCTTT 
GTGCTCATCG CCCTGTGCTG CATTCCTTGG ATGCTTCTGG GCAAGCCCCT GTACATCAAG 
TTCACTCGCA AAAACAAGGC TCATGCCAAT CACAATGGTC AGTTGACCGG CAACATTGAA 
CTGGCCGAAG GCGAGACTCC TCTGCCCACA GGATTCTCTG GAAACGAGGA GAATGCCGGG 
GGTGCCCATG GCCATGACGA TGAGCCCATG AGCGAAATCT ACATCCATCA AGCCATCCAC 
ACCATCGAAT ATGTGCTCAG TACCATCTCG CACACGGCGT CCTATCTGCG TCTCTGGGCT 
CTGTCCCTGG CTCACGCCCA GCTCTCCGAG GTGCTGTGGC AAATGGTGTT GTCCCTGGGC 
CTTAAGATGT CCGGCGTGGG CGGTGCCATT GGTCTGTTCA TCATCTTCGG CGCCTGGTGC 
TTGTTCACCC TGGCTATCCT GGTCCTCATG GAGGGTCTGT CCGCCTTCCT GCACACTCTG 
CGTCTGCACT GGGTGGAGTT CATGAGCAAG TTCTACGAGG GAATGGGCTA CGCCTTCCAG 
CCGTTCAGCT TCAAGGCCAT TCTCGATGGC GAAGAGGAGG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Vha100-2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

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ORF Insert Sequence:

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