Ns1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ns1
Entrez Gene ID	42060
Full Name	Nucleostemin 1
Synonyms	CG3983,Dmel\CG3983,NS1,dmNS1,ns1
General protein information	Preferred Names Nucleostemin 1 Names CG3983 gene product from transcript CG3983-RA CG3983-PA Ns1-PA nucleostemin 1 nucleostemin ortholog 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 89E11-89E11

mRNA and Protein(s)

mRNA	Protein	Name
NM_169742.3	NP_732199.2	nucleostemin 1

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os01g0375000	NP_001043076.1
Drosophila melanogaster (fruit fly)	ns1	NP_732199.2
Danio rerio (zebrafish)	gnl3l	NP_001002875.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004514	XP_313813.5
Caenorhabditis elegans (roundworm)	nst-1	NP_495749.1
Arabidopsis thaliana (thale cress)	AT3G07050	NP_187361.1
Xenopus tropicalis (tropical clawed frog)	LOC100489738	XP_002935538.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ns1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ns1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05188	NM_169742.3 Latest version!	Drosophila melanogaster nucleostemin 1 (Ns1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ns1 ( NM_169742.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732199.2 Drosophila melanogaster nucleostemin 1 (Ns1), mRNA.

Price & Availability↑

CloneID	OFa05188
Clone ID Related Accession (Same CDS sequence)	NM_169742.3
Accession Version	NM_169742.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	nucleostemin 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169742.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTTTAA AAAGGTTGAA GACCAAGAAA TCCAAGAGGC TCACCGGTCG CTTGAAGCAC 
AAGATCGAAA AGAAGGTGCG GGACCACAAC AAAAAGGAAC GTCGCGCAGC CAAGAAGAAT 
CCAAAGAAGG GTAGCAAGAA ACAGAAGCTC ATCCAGATCC CAAACATCTG TCCCTTCAAG 
GATGACATCC TCAAGGAGGT GGAGGAGGCC AAGCAACGTC AGGAGGCCGA ACGTTTGGCT 
CGCCGCGAAG CCTTTAAGGC GGAACGCGAA CAGAACAAGT TCAAGACGCT GGAGTCCATG 
GTCGAGGATG CAGACATGCG GAGCACCGTG CACGGAATAA TGCACGAAAA CGATGCCCAG 
GATCAGGACG AAAAGAAGTA CAAGAACGCC GTGACCAAAG AGCAGTCATT GAAGCAGTAC 
TTCAAGGAAT TCCGCAAGGT GATAGAAAAT GCCGACGTTG TCCTGGAGGT GGTTGATGCA 
CGTGACCCCC TGGGAACCCG CTGCAATGAG GTTGAGCGCG CCGTTCGCGG TGCTCCGGGC 
AACAAGCGAC TGGTCCTGGT GCTCAACAAA GCCGACCTGG TGCCGCGAGA GAATCTGAAC 
AACTGGATCA AGTACTTCCG TCGCAGCGGA CCTGTCACCG CCTTCAAGGC TTCCACACAG 
GATCAGGCCA ACCGGCTGGG ACGCCGCAAA CTCCGCGAAA TGAAAACCGA GAAGGCTATG 
CAGGGTTCTG TCTGCATTGG CGCTGAGTTG CTTATGTCCA TGCTGGGCAA CTACTGCCGC 
AACAAGGGCA TCAAGACCTC GATTCGCGTT GGCGTCGTTG GAATTCCTAA TGTAGGCAAG 
AGCTCCATCA TAAACTCGTT GACCCGCGGC CGGTCCTGCA TGGTGGGCAG CACGCCAGGA 
GTGACCAAAT CAATGCAAGA AGTAGAACTG GATTCGAAAA TCAAACTGAT CGATTGTCCT 
GGAATCGTAT TTACCAGCGG AGGTGAAAAC TCGCATGCAG TTCTGAAGAA TGCACAGCGT 
GTGGGCGATG TAAAGGATCC TTTCACCATT GCCGAGAGCG TATTGAAGCG TGCCAGCAAG 
GAATACTTCT GTACGATGTA CGATATTACA AACTACGACA CATTTGAGGA ATTCTTCGCC 
AAAAAGGCAG CCAGAATGGG CAAATTTCTA AAGAAGGGAG TGCCAGATGT TGTGGCTGCA 
GCAAGAAGCG TTCTCAACGA TTGGAACACG GGCAAAATTA AATACTGCAC ACAGCCTCCG 
GAAGTTCAGG AAGGGCAAAG TGTGCATATA AGTGCCTCGA TAGTACACTC AGAGGCTCGC 
GAATTTGATG TGGAGAACTT TGAGTCCATG GAGACGGAAA TCTTAGAGCA TTGCGCCGTG 
AAAACGGATG ACATTATGGA GATAACGTCT ACGGGGCCTT TAGAGATTCG ACAGCCGCGT 
GAGGAAGCCG AACCTGCAGA TAAAATAACT GCCAGTTTGG TAATAGACGA GAAGGAAAAG 
CCCGCAAAGG GTCGCAAACG AAAACTGGAT GAGGAAAAGG AGAAGGTTGA TCCCAGTCTA 
CTCCTAGAAG AAAACCAATC ACTGAATAAA GGCATAAAGC AGATGCAAAA GCTAAAAAAG 
AAACAGAATG TTCGCAACGA GAAGAAAATA TCCAAAATTA CAGACGTTCT GGATAGCTTT 
AGCTTAGGTC CATCCTCGTC CAAGGCAGAA AAATACGATT TTGATGAGGA CTATGTGATT 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732199.2
CDS	73..1818
Translation	MALKRLKTKKSKRLTGRLKHKIEKKVRDHNKKERRAAKKNPKKGSKKQKLIQIPNICPFKDDILKEVEEAKQRQEAERLARREAFKAEREQNKFKTLESMVEDADMRSTVHGIMHENDAQDQDEKKYKNAVTKEQSLKQYFKEFRKVIENADVVLEVVDARDPLGTRCNEVERAVRGAPGNKRLVLVLNKADLVPRENLNNWIKYFRRSGPVTAFKASTQDQANRLGRRKLREMKTEKAMQGSVCIGAELLMSMLGNYCRNKGIKTSIRVGVVGIPNVGKSSIINSLTRGRSCMVGSTPGVTKSMQEVELDSKIKLIDCPGIVFTSGGENSHAVLKNAQRVGDVKDPFTIAESVLKRASKEYFCTMYDITNYDTFEEFFAKKAARMGKFLKKGVPDVVAAARSVLNDWNTGKIKYCTQPPEVQEGQSVHISASIVHSEAREFDVENFESMETEILEHCAVKTDDIMEITSTGPLEIRQPREEAEPADKITASLVIDEKEKPAKGRKRKLDEEKEKVDPSLLLEENQSLNKGIKQMQKLKKKQNVRNEKKISKITDVLDSFSLGPSSSKAEKYDFDEDYVIE

Target ORF information:

RefSeq Version	NM_169742.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleostemin 1 (Ns1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169742.3

ORF Insert Sequence:

ATGGCTTTAA AAAGGTTGAA GACCAAGAAA TCCAAGAGGC TCACCGGTCG CTTGAAGCAC 
AAGATCGAAA AGAAGGTGCG GGACCACAAC AAAAAGGAAC GTCGCGCAGC CAAGAAGAAT 
CCAAAGAAGG GTAGCAAGAA ACAGAAGCTC ATCCAGATCC CAAACATCTG TCCCTTCAAG 
GATGACATCC TCAAGGAGGT GGAGGAGGCC AAGCAACGTC AGGAGGCCGA ACGTTTGGCT 
CGCCGCGAAG CCTTTAAGGC GGAACGCGAA CAGAACAAGT TCAAGACGCT GGAGTCCATG 
GTCGAGGATG CAGACATGCG GAGCACCGTG CACGGAATAA TGCACGAAAA CGATGCCCAG 
GATCAGGACG AAAAGAAGTA CAAGAACGCC GTGACCAAAG AGCAGTCATT GAAGCAGTAC 
TTCAAGGAAT TCCGCAAGGT GATAGAAAAT GCCGACGTTG TCCTGGAGGT GGTTGATGCA 
CGTGACCCCC TGGGAACCCG CTGCAATGAG GTTGAGCGCG CCGTTCGCGG TGCTCCGGGC 
AACAAGCGAC TGGTCCTGGT GCTCAACAAA GCCGACCTGG TGCCGCGAGA GAATCTGAAC 
AACTGGATCA AGTACTTCCG TCGCAGCGGA CCTGTCACCG CCTTCAAGGC TTCCACACAG 
GATCAGGCCA ACCGGCTGGG ACGCCGCAAA CTCCGCGAAA TGAAAACCGA GAAGGCTATG 
CAGGGTTCTG TCTGCATTGG CGCTGAGTTG CTTATGTCCA TGCTGGGCAA CTACTGCCGC 
AACAAGGGCA TCAAGACCTC GATTCGCGTT GGCGTCGTTG GAATTCCTAA TGTAGGCAAG 
AGCTCCATCA TAAACTCGTT GACCCGCGGC CGGTCCTGCA TGGTGGGCAG CACGCCAGGA 
GTGACCAAAT CAATGCAAGA AGTAGAACTG GATTCGAAAA TCAAACTGAT CGATTGTCCT 
GGAATCGTAT TTACCAGCGG AGGTGAAAAC TCGCATGCAG TTCTGAAGAA TGCACAGCGT 
GTGGGCGATG TAAAGGATCC TTTCACCATT GCCGAGAGCG TATTGAAGCG TGCCAGCAAG 
GAATACTTCT GTACGATGTA CGATATTACA AACTACGACA CATTTGAGGA ATTCTTCGCC 
AAAAAGGCAG CCAGAATGGG CAAATTTCTA AAGAAGGGAG TGCCAGATGT TGTGGCTGCA 
GCAAGAAGCG TTCTCAACGA TTGGAACACG GGCAAAATTA AATACTGCAC ACAGCCTCCG 
GAAGTTCAGG AAGGGCAAAG TGTGCATATA AGTGCCTCGA TAGTACACTC AGAGGCTCGC 
GAATTTGATG TGGAGAACTT TGAGTCCATG GAGACGGAAA TCTTAGAGCA TTGCGCCGTG 
AAAACGGATG ACATTATGGA GATAACGTCT ACGGGGCCTT TAGAGATTCG ACAGCCGCGT 
GAGGAAGCCG AACCTGCAGA TAAAATAACT GCCAGTTTGG TAATAGACGA GAAGGAAAAG 
CCCGCAAAGG GTCGCAAACG AAAACTGGAT GAGGAAAAGG AGAAGGTTGA TCCCAGTCTA 
CTCCTAGAAG AAAACCAATC ACTGAATAAA GGCATAAAGC AGATGCAAAA GCTAAAAAAG 
AAACAGAATG TTCGCAACGA GAAGAAAATA TCCAAAATTA CAGACGTTCT GGATAGCTTT 
AGCTTAGGTC CATCCTCGTC CAAGGCAGAA AAATACGATT TTGATGAGGA CTATGTGATT 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ns1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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