Vps45 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vps45
Entrez Gene ID	41153
Full Name	Vacuolar protein sorting 45
Synonyms	CG8228,DmVps45,Dmel\CG8228,MENE (3R)-C,MENE(3R)-C,VPS45,Vps45p,dVps45
General protein information	Preferred Names Vacuolar protein sorting 45 Names CG8228 gene product from transcript CG8228-RA CG8228-PA Vps45-PA vacuolar protein sorting 45
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 85D25-85D25

mRNA and Protein(s)

mRNA	Protein	Name
NM_141652.2	NP_649909.1	vacuolar protein sorting 45

Pathways from BioSystems

Homologies

Homo sapiens (human)	VPS45	NP_009190.2
Canis lupus familiaris (dog)	VPS45	XP_533042.2
Bos taurus (cattle)	VPS45	NP_001076970.1
Mus musculus (house mouse)	Vps45	NP_038869.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003980	XP_318430.3
Neurospora crassa	NCU06192	XP_963004.1
Schizosaccharomyces pombe (fission yeast)	vps45	NP_593083.1
Danio rerio (zebrafish)	vps45	NP_001243585.1
Eremothecium gossypii	AGOS_AFL053W	NP_985495.2
Rattus norvegicus (Norway rat)	Vps45	NP_742069.1
Saccharomyces cerevisiae (baker's yeast)	VPS45	NP_011420.3
Arabidopsis thaliana (thale cress)	VPS45	NP_565150.1
Magnaporthe oryzae (rice blast fungus)	MGG_14755	XP_003713874.1
Kluyveromyces lactis	KLLA0E18833g	XP_454802.1
Pan troglodytes (chimpanzee)	VPS45	XP_001167625.1
Macaca mulatta (Rhesus monkey)	VPS45	XP_001098282.1
Drosophila melanogaster (fruit fly)	Vps45	NP_649909.1
Caenorhabditis elegans (roundworm)	vps-45	NP_741714.1
Xenopus tropicalis (tropical clawed frog)	vps45	NP_001072504.1
Oryza sativa (rice)	Os02g0437800	NP_001046739.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Vps45 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vps45 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04341	NM_141652.2 Latest version!	Drosophila melanogaster vacuolar protein sorting 45 (Vps45), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vps45 ( NM_141652.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649909.1 Drosophila melanogaster vacuolar protein sorting 45 (Vps45), mRNA.

Price & Availability↑

CloneID	OFa04341
Clone ID Related Accession (Same CDS sequence)	NM_141652.2
Accession Version	NM_141652.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar protein sorting 45
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141652.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCTGA TAAGCGGCAT TAAGTTGTAC ATCGAGAAAA TGTGCTCCGA GTCGGGACCG 
GGCATGAAAA TCATTCTGCT GGACAAGGAG ACGACCAGTA TAATTTCGAT GGCCTTTAGC 
CAATCGGACA TGCTGCAACG GGAGGTGTAC CTATTTGAGC GCCTGGACTC GGGAAGATCC 
AACGAGCGGT TAAAGTATCT AAAATGCATC GTCTTTATAC GCCCCACCAA GCAGAACATC 
CAGCTACTGG CCAACGAGTT GCGGAACCCC AAATACAGCG CCTACTACAT TTATTTCAGC 
AACATCATTC CCCGGACGGA CATTAAATAC TTGGCAGAGT GCGACGAATC CGAATCGGTG 
CGAGAAGTGA AGGAACTGTA TGCAGACTAC CTGTGCGTCA ACCCCAATCT GTTCTCATTA 
GGCATTCCCA ATTGCATGGC GAATCTTAAT TGGCTGCCGG ACGCTCTCAA CCGCAGCATG 
CAGGGCATCA CAGCCGTGCT GTTGTCCCTT AAGCTGAACC CAGTGATCCG GTACCGAGCC 
GGTTCTCAGG CAGCGCAGCT TCTGGCCAAG CTCATATACG AGCAAATCAC AAAAGAGTCC 
TCGCTATTCG ACTTTCGCTC CAACATGGAT GGCGCTGCGC CACCGCTGCT GCTTGTGCTG 
GACCGCAGGG ACGATCCTGT GACGCCGCTT TTGCATCAGT GGACATACCA GGCCATGGTG 
CATGAGTTGC TGCACATAAA AAACAATCGA TTGGACCTGT CCAATTGCCC CAATGTGCCG 
AAAGACTTCA AGGAGCTGGT CTTATCCGGT GATCAGGATG ACTTCTATGG CAACAACATG 
TACGCCAACT ACGGCGAAAT CGGATCTACC ATCAAACAAC TGATGGAGGA GTTCCAGCGC 
AAGGCGAACG ACCACAAGAA AGTGGAGAGC ATAGCAGACA TGAAGAACTT TATTGAGTCA 
TATCCGCAGT TTAAAAAAAT GTCTGGCACT GTGCAAAAGC ATTTGTGTGT GATTGGAGAA 
CTGTCGGCGC TGAGCAACAA GAGGAATCTC TTCGAGGTTT CCGAACTTGA ACAGGAGATC 
GCTTGCAAGG CGGAGCACTC GGCCCAGCTG CAGAGAATCA AAAAACTAAT TGCCGACGAA 
CGGGTTTCCA TCGATGATGC TCTTAAGTTG GTGGCACTTT ATGCATTGCG ATACGAGCGA 
CACGCCAACT GCGACACATC TGGACTGCTG CAAATAATCA AAACGCGCGG TGGACGAGCG 
GCTATAGTGC CATCGTTGAT TGAGTATGCT GGAACCCATG TACGACAAGG AGATCTCTTC 
AATATGGTGC GGATAACGGA CGCCGTAAAG CTGACACGGA ATTTGATTAA GGGCCTTAAG 
GGTGTGGAAA ATGTCTTCAC ACAGCACACT CCTCTCCTGA AGGAAACTTT GGAGGACGTG 
TTTAAGGGCC GCGAGCTGGA TCCCCTCTTC CCGGCCATCA ATTCAGAGCT TGTGCCATTT 
CGACGACCAC CGCAGGAGGT TGTCGTGTTC ATTATTGGCG GTGCCACGTA CGAGGAAGCC 
TTAGCGGTGC ACCAGTTAAA CAACGCCGGC TATAAAGTCA TTCTTGGCGG CACCACCATC 
CATAACTCGC AGAGTTTTAT CCAAGAGGTC GTGGCCGCCA CTAGTGGCAT TCAGTTTAAG 
CACACCAAAT CCATGATCAA GTACTGTTCC ACGGATAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649909.1
CDS	103..1827
Translation	MNLISGIKLYIEKMCSESGPGMKIILLDKETTSIISMAFSQSDMLQREVYLFERLDSGRSNERLKYLKCIVFIRPTKQNIQLLANELRNPKYSAYYIYFSNIIPRTDIKYLAECDESESVREVKELYADYLCVNPNLFSLGIPNCMANLNWLPDALNRSMQGITAVLLSLKLNPVIRYRAGSQAAQLLAKLIYEQITKESSLFDFRSNMDGAAPPLLLVLDRRDDPVTPLLHQWTYQAMVHELLHIKNNRLDLSNCPNVPKDFKELVLSGDQDDFYGNNMYANYGEIGSTIKQLMEEFQRKANDHKKVESIADMKNFIESYPQFKKMSGTVQKHLCVIGELSALSNKRNLFEVSELEQEIACKAEHSAQLQRIKKLIADERVSIDDALKLVALYALRYERHANCDTSGLLQIIKTRGGRAAIVPSLIEYAGTHVRQGDLFNMVRITDAVKLTRNLIKGLKGVENVFTQHTPLLKETLEDVFKGRELDPLFPAINSELVPFRRPPQEVVVFIIGGATYEEALAVHQLNNAGYKVILGGTTIHNSQSFIQEVVAATSGIQFKHTKSMIKYCSTDNI

Target ORF information:

RefSeq Version	NM_141652.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar protein sorting 45 (Vps45), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141652.2

ORF Insert Sequence:

ATGAATCTGA TAAGCGGCAT TAAGTTGTAC ATCGAGAAAA TGTGCTCCGA GTCGGGACCG 
GGCATGAAAA TCATTCTGCT GGACAAGGAG ACGACCAGTA TAATTTCGAT GGCCTTTAGC 
CAATCGGACA TGCTGCAACG GGAGGTGTAC CTATTTGAGC GCCTGGACTC GGGAAGATCC 
AACGAGCGGT TAAAGTATCT AAAATGCATC GTCTTTATAC GCCCCACCAA GCAGAACATC 
CAGCTACTGG CCAACGAGTT GCGGAACCCC AAATACAGCG CCTACTACAT TTATTTCAGC 
AACATCATTC CCCGGACGGA CATTAAATAC TTGGCAGAGT GCGACGAATC CGAATCGGTG 
CGAGAAGTGA AGGAACTGTA TGCAGACTAC CTGTGCGTCA ACCCCAATCT GTTCTCATTA 
GGCATTCCCA ATTGCATGGC GAATCTTAAT TGGCTGCCGG ACGCTCTCAA CCGCAGCATG 
CAGGGCATCA CAGCCGTGCT GTTGTCCCTT AAGCTGAACC CAGTGATCCG GTACCGAGCC 
GGTTCTCAGG CAGCGCAGCT TCTGGCCAAG CTCATATACG AGCAAATCAC AAAAGAGTCC 
TCGCTATTCG ACTTTCGCTC CAACATGGAT GGCGCTGCGC CACCGCTGCT GCTTGTGCTG 
GACCGCAGGG ACGATCCTGT GACGCCGCTT TTGCATCAGT GGACATACCA GGCCATGGTG 
CATGAGTTGC TGCACATAAA AAACAATCGA TTGGACCTGT CCAATTGCCC CAATGTGCCG 
AAAGACTTCA AGGAGCTGGT CTTATCCGGT GATCAGGATG ACTTCTATGG CAACAACATG 
TACGCCAACT ACGGCGAAAT CGGATCTACC ATCAAACAAC TGATGGAGGA GTTCCAGCGC 
AAGGCGAACG ACCACAAGAA AGTGGAGAGC ATAGCAGACA TGAAGAACTT TATTGAGTCA 
TATCCGCAGT TTAAAAAAAT GTCTGGCACT GTGCAAAAGC ATTTGTGTGT GATTGGAGAA 
CTGTCGGCGC TGAGCAACAA GAGGAATCTC TTCGAGGTTT CCGAACTTGA ACAGGAGATC 
GCTTGCAAGG CGGAGCACTC GGCCCAGCTG CAGAGAATCA AAAAACTAAT TGCCGACGAA 
CGGGTTTCCA TCGATGATGC TCTTAAGTTG GTGGCACTTT ATGCATTGCG ATACGAGCGA 
CACGCCAACT GCGACACATC TGGACTGCTG CAAATAATCA AAACGCGCGG TGGACGAGCG 
GCTATAGTGC CATCGTTGAT TGAGTATGCT GGAACCCATG TACGACAAGG AGATCTCTTC 
AATATGGTGC GGATAACGGA CGCCGTAAAG CTGACACGGA ATTTGATTAA GGGCCTTAAG 
GGTGTGGAAA ATGTCTTCAC ACAGCACACT CCTCTCCTGA AGGAAACTTT GGAGGACGTG 
TTTAAGGGCC GCGAGCTGGA TCCCCTCTTC CCGGCCATCA ATTCAGAGCT TGTGCCATTT 
CGACGACCAC CGCAGGAGGT TGTCGTGTTC ATTATTGGCG GTGCCACGTA CGAGGAAGCC 
TTAGCGGTGC ACCAGTTAAA CAACGCCGGC TATAAAGTCA TTCTTGGCGG CACCACCATC 
CATAACTCGC AGAGTTTTAT CCAAGAGGTC GTGGCCGCCA CTAGTGGCAT TCAGTTTAAG 
CACACCAAAT CCATGATCAA GTACTGTTCC ACGGATAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Vps45 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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