Vps16A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vps16A
Entrez Gene ID	41107
Full Name	Vacuolar protein sorting 16A
Synonyms	CG8454,DmVps16,Dmel\CG8454,V16,Vps16,dVps16A,vps16A
General protein information	Preferred Names Vacuolar protein sorting 16A Names CG8454 gene product from transcript CG8454-RA CG8454-PA Vps16 Vps16A-PA vacuolar protein sorting 16A
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 85D7-85D7

mRNA and Protein(s)

mRNA	Protein	Name
NM_141620.3	NP_649877.1	vacuolar protein sorting 16A

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	VCL1	NP_565879.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000529	XP_310557.3
Rattus norvegicus (Norway rat)	Vps16	NP_001005541.1
Danio rerio (zebrafish)	vps16	NP_001091659.1
Drosophila melanogaster (fruit fly)	Vps16A	NP_649877.1
Neurospora crassa	NCU06268	XP_962872.2
Schizosaccharomyces pombe (fission yeast)	vps16	NP_593444.1
Oryza sativa (rice)	Os01g0666800	NP_001043804.2
Homo sapiens (human)	VPS16	NP_072097.2
Bos taurus (cattle)	VPS16	NP_001015522.1
Gallus gallus (chicken)	VPS16	NP_001258079.1
Xenopus tropicalis (tropical clawed frog)	vps16	NP_001004976.1
Magnaporthe oryzae (rice blast fungus)	MGG_05256	XP_003712813.1
Mus musculus (house mouse)	Vps16	NP_085036.3
Canis lupus familiaris (dog)	VPS16	XP_003639898.1
Macaca mulatta (Rhesus monkey)	VPS16	XP_002798262.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Vps16A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vps16A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08185	NM_141620.3 Latest version!	Drosophila melanogaster vacuolar protein sorting 16A (Vps16A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vps16A ( NM_141620.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649877.1 Drosophila melanogaster vacuolar protein sorting 16A (Vps16A), mRNA.

Price & Availability↑

CloneID	OFa08185
Clone ID Related Accession (Same CDS sequence)	NM_141620.3
Accession Version	NM_141620.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar protein sorting 16A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_141620.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCTATCA TGTACAACAC GGGGGAGTGG TTCAAGGTGC GACCGGACTA CTACAGAAAA 
GTGGAACTGG CTACGCCTGA TTGGCCTCTG GACCTGGACT TGGAGTATAT GTGGGTGGAA 
GTGGCTCCCT ATGGAGGACC ACTGGCGGTC ACTCGTGATC CGACCAAATT GGTGCCGGTA 
AAGGGTAACG CTAGACCCAT GATTCGCATC TTTGATACCA CCGGCAGGGA AATGGGTCAC 
ATATTGTGGA ACCATGGCAA ACTTATTGCC ATGGGCTGGT CAGACATGGA GGAGCTTATC 
TGCATCCAGG AGAACGCCAC CGTCTTTGTC TACGACATGT TTGGTCGGGA AAAGGAGTCC 
TACAGCATCG GCGATGAGGC CAGTGTCACA AAGATTGTGG AGGGCAAGGT GTTCCAGTCC 
TCAGCTGGCA CAGGAGTGGC TGTAATGACC ACATCCGGTC GGGTTTTCCT CAAGCAGAAC 
AGCAGCAAGA CTGAAAGAAA GCTGCCGGAT ATTCCCAATT CCAGCATGAA CTGTTCCTGC 
TGGGAGATCG TAACCGAGGG ACGCAACAGC TACTGTCTGC TGGGAAGGGA TCGAGAGGTC 
ATAAAGCTGT TTCCAGGCGA AACTGTGGGA ACTATTACCG CCAATCTCTT TGAGAAACCG 
CACGAGCGAA TCATAAAGAT ATCTGTGTCG TACAACCATC AGCACTTGGC ACTGTACACC 
AACACCGGTC TTCTTTGGCT GGGCAGCGTA GATATGCGGC AAAAGTATTG TGAATTCGAC 
ACCGGACGTA AAGATATGCC CCTCCAAATG GAGTGGATCA TGAACTCTGA CAACAGCGAG 
GCGGATGCGG TGGTCATTTC ATATCCATCG TACCTCTTAA TTGTAAATCG TAACGCTGAT 
CGCAGTGACT TTCCCTACGA TCCGGTGATG TTTCTTGTGG CTGAAATGGA TGGCGTTCGC 
ATTATTACTC AGAGTTCGCA TGAAATAATA CAGCGCCTGC CAAAATGCGT TGAGAACATA 
TTTGCAGTGA ATAGCCAGGA ACCGGCTAGC TATCTCTTTG AGGCACAGAA AAAGTTTGAG 
GAGAAGTCCT ACAAGTCGGA TGAGTATCTC AGCATGTGTC GGGAAAAGAT CGATCTGGCA 
GTGTCCGAAT GTATTGAGGC GGCTTCATAT GAGTTTTGTC CGGAAACTCA AAAGAGCTTG 
CTTAGGACGG CCTATTTTGG AAAAGGCTTT ATTCGTAACC ACAATCCAGA TGAATATATG 
CGTATTATGC GAATACTGAG GGTTCTTAAC ACCTTGCGGC ATGAGCGAAT TGCCATGCCC 
ATCACCTTCA AACAATTCTC ACATCTTAAT ACCGAGGTCA TACTCAGCCG TTTGGTATTC 
CGCAAACACT ATGCCATTGC GATACAAGTG GCCAAGCATT TAAATCTGCC CGAATCGTGG 
ATCTTGGAAC ACTGGGCTTA CCATAAAGTG ATGAATGATC CCAATGATAC TGAAGTGGCG 
CGTAAGATAA CTGAAAAGTT CAAGAATCCG TCGGTCGAGG GAATTTCTTT CTGCAATATT 
GCGTCAAAGG CGCATCAAGC TGGACGAGAT GACCTGGCCA TTAAGCTGCT AGAGCTCGAG 
TCTCGTGCTT CGCTTCACGT ACCACTACTT CTGAAGATGC GCAAGTTTGA TCGGGCGGTG 
GGCAGTGCCA CACAGTCCGG CGATACAGAG TTGATCACCC AAGTTCTCTT GGAGATGAAA 
ATGCACATGA TGCTGTCCAA TTTGCACATG ACTATTAGGG ACCATCCACT GGCTCTAAAC 
ATATACAAAA AGATAATGCG GGAGTCCAAC AGAGCTGGAC TCTATGGGAT TTACAACACC 
GAAGACGACC AAAAGGCCAT CGCTGAGTAT CACTTCCAGA ATGCCATTGA AACTGAAGGA 
TTGGAGTCGA ATCTTTCGAT GATTGGAAAC GCCTATGCCC AGGGCCGTTG CACATTGGAA 
GCGGAGCTTT GTGCGGATAC CAGCCGATTG ATTAAGCTGC AAAAGACTCT AAGCACCAAG 
TATAATGTCA GCTTAAATGG CTTGTCAATA CATGAGACCA TTCAAGAATT ACTGCTGATC 
GGCGAATTGA AAGAGGCAGA GCGAATCAGA AGCGAATTTA AAGTGCCCGA TCGTCGGTTC 
TGGTGGCTAC GAATCCTCAC ACTTTCTAGT CAACACAAGT GGGAGGAGTT GGAAAAGTTA 
GCTAAAAGTA AAAAGAGCCC CATTGGCTAC GATCCTTTTG TTGAGGTCTG CTTAAAACAG 
GACAATGTAA TGGAGGCCCG AAAGTATATA CCAAGGTGTC GGGACAATCG CCGGGTCGTC 
TGGTATATGC GAGCAAACCT ATTTAACGAA GCCATAGATT CCGCCTTCGA GCAGCGTGAT 
GTGCACAGTC TCTTTGAGTT GCAAAAGAAC CAGGCGATCG TTAACAATGG CACACTGTTG 
AGCAAAGTTT GCGATTGCAT TGCCATGCTG GAGGGAAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649877.1
CDS	104..2605
Translation	MPIMYNTGEWFKVRPDYYRKVELATPDWPLDLDLEYMWVEVAPYGGPLAVTRDPTKLVPVKGNARPMIRIFDTTGREMGHILWNHGKLIAMGWSDMEELICIQENATVFVYDMFGREKESYSIGDEASVTKIVEGKVFQSSAGTGVAVMTTSGRVFLKQNSSKTERKLPDIPNSSMNCSCWEIVTEGRNSYCLLGRDREVIKLFPGETVGTITANLFEKPHERIIKISVSYNHQHLALYTNTGLLWLGSVDMRQKYCEFDTGRKDMPLQMEWIMNSDNSEADAVVISYPSYLLIVNRNADRSDFPYDPVMFLVAEMDGVRIITQSSHEIIQRLPKCVENIFAVNSQEPASYLFEAQKKFEEKSYKSDEYLSMCREKIDLAVSECIEAASYEFCPETQKSLLRTAYFGKGFIRNHNPDEYMRIMRILRVLNTLRHERIAMPITFKQFSHLNTEVILSRLVFRKHYAIAIQVAKHLNLPESWILEHWAYHKVMNDPNDTEVARKITEKFKNPSVEGISFCNIASKAHQAGRDDLAIKLLELESRASLHVPLLLKMRKFDRAVGSATQSGDTELITQVLLEMKMHMMLSNLHMTIRDHPLALNIYKKIMRESNRAGLYGIYNTEDDQKAIAEYHFQNAIETEGLESNLSMIGNAYAQGRCTLEAELCADTSRLIKLQKTLSTKYNVSLNGLSIHETIQELLLIGELKEAERIRSEFKVPDRRFWWLRILTLSSQHKWEELEKLAKSKKSPIGYDPFVEVCLKQDNVMEARKYIPRCRDNRRVVWYMRANLFNEAIDSAFEQRDVHSLFELQKNQAIVNNGTLLSKVCDCIAMLEGK

Target ORF information:

RefSeq Version	NM_141620.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar protein sorting 16A (Vps16A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141620.3

ORF Insert Sequence:

ATGCCTATCA TGTACAACAC GGGGGAGTGG TTCAAGGTGC GACCGGACTA CTACAGAAAA 
GTGGAACTGG CTACGCCTGA TTGGCCTCTG GACCTGGACT TGGAGTATAT GTGGGTGGAA 
GTGGCTCCCT ATGGAGGACC ACTGGCGGTC ACTCGTGATC CGACCAAATT GGTGCCGGTA 
AAGGGTAACG CTAGACCCAT GATTCGCATC TTTGATACCA CCGGCAGGGA AATGGGTCAC 
ATATTGTGGA ACCATGGCAA ACTTATTGCC ATGGGCTGGT CAGACATGGA GGAGCTTATC 
TGCATCCAGG AGAACGCCAC CGTCTTTGTC TACGACATGT TTGGTCGGGA AAAGGAGTCC 
TACAGCATCG GCGATGAGGC CAGTGTCACA AAGATTGTGG AGGGCAAGGT GTTCCAGTCC 
TCAGCTGGCA CAGGAGTGGC TGTAATGACC ACATCCGGTC GGGTTTTCCT CAAGCAGAAC 
AGCAGCAAGA CTGAAAGAAA GCTGCCGGAT ATTCCCAATT CCAGCATGAA CTGTTCCTGC 
TGGGAGATCG TAACCGAGGG ACGCAACAGC TACTGTCTGC TGGGAAGGGA TCGAGAGGTC 
ATAAAGCTGT TTCCAGGCGA AACTGTGGGA ACTATTACCG CCAATCTCTT TGAGAAACCG 
CACGAGCGAA TCATAAAGAT ATCTGTGTCG TACAACCATC AGCACTTGGC ACTGTACACC 
AACACCGGTC TTCTTTGGCT GGGCAGCGTA GATATGCGGC AAAAGTATTG TGAATTCGAC 
ACCGGACGTA AAGATATGCC CCTCCAAATG GAGTGGATCA TGAACTCTGA CAACAGCGAG 
GCGGATGCGG TGGTCATTTC ATATCCATCG TACCTCTTAA TTGTAAATCG TAACGCTGAT 
CGCAGTGACT TTCCCTACGA TCCGGTGATG TTTCTTGTGG CTGAAATGGA TGGCGTTCGC 
ATTATTACTC AGAGTTCGCA TGAAATAATA CAGCGCCTGC CAAAATGCGT TGAGAACATA 
TTTGCAGTGA ATAGCCAGGA ACCGGCTAGC TATCTCTTTG AGGCACAGAA AAAGTTTGAG 
GAGAAGTCCT ACAAGTCGGA TGAGTATCTC AGCATGTGTC GGGAAAAGAT CGATCTGGCA 
GTGTCCGAAT GTATTGAGGC GGCTTCATAT GAGTTTTGTC CGGAAACTCA AAAGAGCTTG 
CTTAGGACGG CCTATTTTGG AAAAGGCTTT ATTCGTAACC ACAATCCAGA TGAATATATG 
CGTATTATGC GAATACTGAG GGTTCTTAAC ACCTTGCGGC ATGAGCGAAT TGCCATGCCC 
ATCACCTTCA AACAATTCTC ACATCTTAAT ACCGAGGTCA TACTCAGCCG TTTGGTATTC 
CGCAAACACT ATGCCATTGC GATACAAGTG GCCAAGCATT TAAATCTGCC CGAATCGTGG 
ATCTTGGAAC ACTGGGCTTA CCATAAAGTG ATGAATGATC CCAATGATAC TGAAGTGGCG 
CGTAAGATAA CTGAAAAGTT CAAGAATCCG TCGGTCGAGG GAATTTCTTT CTGCAATATT 
GCGTCAAAGG CGCATCAAGC TGGACGAGAT GACCTGGCCA TTAAGCTGCT AGAGCTCGAG 
TCTCGTGCTT CGCTTCACGT ACCACTACTT CTGAAGATGC GCAAGTTTGA TCGGGCGGTG 
GGCAGTGCCA CACAGTCCGG CGATACAGAG TTGATCACCC AAGTTCTCTT GGAGATGAAA 
ATGCACATGA TGCTGTCCAA TTTGCACATG ACTATTAGGG ACCATCCACT GGCTCTAAAC 
ATATACAAAA AGATAATGCG GGAGTCCAAC AGAGCTGGAC TCTATGGGAT TTACAACACC 
GAAGACGACC AAAAGGCCAT CGCTGAGTAT CACTTCCAGA ATGCCATTGA AACTGAAGGA 
TTGGAGTCGA ATCTTTCGAT GATTGGAAAC GCCTATGCCC AGGGCCGTTG CACATTGGAA 
GCGGAGCTTT GTGCGGATAC CAGCCGATTG ATTAAGCTGC AAAAGACTCT AAGCACCAAG 
TATAATGTCA GCTTAAATGG CTTGTCAATA CATGAGACCA TTCAAGAATT ACTGCTGATC 
GGCGAATTGA AAGAGGCAGA GCGAATCAGA AGCGAATTTA AAGTGCCCGA TCGTCGGTTC 
TGGTGGCTAC GAATCCTCAC ACTTTCTAGT CAACACAAGT GGGAGGAGTT GGAAAAGTTA 
GCTAAAAGTA AAAAGAGCCC CATTGGCTAC GATCCTTTTG TTGAGGTCTG CTTAAAACAG 
GACAATGTAA TGGAGGCCCG AAAGTATATA CCAAGGTGTC GGGACAATCG CCGGGTCGTC 
TGGTATATGC GAGCAAACCT ATTTAACGAA GCCATAGATT CCGCCTTCGA GCAGCGTGAT 
GTGCACAGTC TCTTTGAGTT GCAAAAGAAC CAGGCGATCG TTAACAATGG CACACTGTTG 
AGCAAAGTTT GCGATTGCAT TGCCATGCTG GAGGGAAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Vps16A cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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