Mkk4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mkk4
Entrez Gene ID	41020
Full Name	MAP kinase kinase 4
Synonyms	142758_at,CG9738,CT27508,D-MKK4,DMKK4,Dmel\CG9738,JNKK2,MAPKK4,MKK4,Mpk4,SEK1/MKK4,dMKK4,dMkk4,hep,mkk4
General protein information	Preferred Names MAP kinase kinase 4 Names CG9738 gene product from transcript CG9738-RC CG9738-PA CG9738-PB CG9738-PC JNK kinase 2 MAPK kinase 4 Map kinase kinase 4 Mkk4-PA Mkk4-PB Mkk4-PC drosophila MAP kinase kinase 4
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 85A5-85A5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001170080.2	NP_001163551.1	MAP kinase kinase 4, isoform B
NM_058005.5	NP_477353.1	MAP kinase kinase 4, isoform A
NM_001275446.1	NP_001262375.1	MAP kinase kinase 4, isoform C

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Map2k4	NP_033183.1
Drosophila melanogaster (fruit fly)	Mkk4	NP_001163551.1
Canis lupus familiaris (dog)	MAP2K4	XP_005620383.1
Bos taurus (cattle)	MAP2K4	NP_001092508.1
Rattus norvegicus (Norway rat)	Map2k4	NP_001025194.1
Danio rerio (zebrafish)	map2k4a	NP_991299.2
Homo sapiens (human)	MAP2K4	NP_001268364.1
Pan troglodytes (chimpanzee)	MAP2K4	XP_001163981.1
Macaca mulatta (Rhesus monkey)	MAP2K4	XP_001114292.1
Gallus gallus (chicken)	MAP2K4	XP_415583.4
Xenopus tropicalis (tropical clawed frog)	map2k4	NP_001107157.1
Schizosaccharomyces pombe (fission yeast)	wis1	NP_595457.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003365	XP_314266.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mkk4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mkk4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04250	NM_001275446.1 Latest version!	Drosophila melanogaster MAP kinase kinase 4 (Mkk4), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa04250	NM_058005.5 Latest version!	Drosophila melanogaster MAP kinase kinase 4 (Mkk4), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa04250	NM_001170080.2 Latest version!	Drosophila melanogaster MAP kinase kinase 4 (Mkk4), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mkk4 ( NM_001170080.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163551.1 Drosophila melanogaster MAP kinase kinase 4 (Mkk4), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa04250
Clone ID Related Accession (Same CDS sequence)	NM_001170080.2 , NM_058005.5 , NM_001275446.1
Accession Version	NM_001170080.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	MAP kinase kinase 4, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170080.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGAAC GACCGAAAAA TTTGTTTGCA ACCGGATCCA GTCGCTCCCG CAATCCCCCG 
GATCAGCTAA GCCTGAATAA TCTTAGCATA CGCCATCCGC CCTCGTCCAC GTCGTCCACT 
TCTTCGGGAT CCACCTCCTC GGGCTCAAGT TCCTCATCAC AGCACAACCA TGTGACCCGC 
TGTTTTGGTG CCCAGCAGCC ACAGCAAACC CCGCCGGTGG CCAGCAGTCA AGTGCCACCG 
GTGCCGGCAG CATCGAGCAG CAGCGCTGCC GATCGTCACC GGGAGCGAAT TCGCCAGCAG 
GCCTGTGGCA AGCTACAGTT TGGTGAGGGC GGCGCTAATA CACACACATT TACTTCAGAC 
GATCTCGAGG ATGAGGGCGA AATTGGACGC GGTGCATTTG GGGCAGTCAA CAAGATGACC 
TTTAAGAAAT TGGACAAGGT GATGGCCGTC AAGCGCATTC GATCCACCGT AGACGAGAAG 
GAACAGAAGC AGCTGCTCAT GGATCTCGAG GTGGTCATGA AGTCCAACGA GTGCATTTAC 
ATTGTTCAGT TCTATGGGGC GCTTTTCAAG GAGGGTGACT GCTGGATTTG TATGGAGCTG 
ATGGACACCT CTTTGGACAA GTTTTACAAG TACATCTACG AAAAGCAGCA GCGCCACATT 
CCAGAGTCTA TACTGGCTAA GATAACAGTG GCCACGGTCA ATGCGCTGAA CTATTTGAAG 
GAGGAGCTAA AGATTATCCA TCGGGATGTG AAACCGAGCA ACATTCTGTT GCACCGTCGC 
GGAGACATAA AGCTGTGCGA TTTTGGTATT TCGGGTCAGC TGGTCGACTC TATTGCCAAG 
ACCAAGGATG CGGGCTGCAG GCCCTACATG GCGCCGGAGC GCATTGATCC AGAACGTGCC 
AAGGGCTACG ATGTACGAAG CGATGTTTGG TCATTGGGCA TCACATTAAT GGAGGTGGCG 
ACTGGTAATT TCCCCTATCG TAAATGGGAC TCTGTTTTCG AGCAGTTGTG CCAGGTTGTG 
CAAGGCGAAC CGCCGCGACT TTTGACATCC TATAACGGCA TGGAGTTTTC CAAAGAGTTT 
GTGGACTTTG TCAATACTTG CCTAATTAAA AAGGAGAGCG ATCGACCCAA GTATAGCCGT 
CTGCTGGAGA TGCCCTTCAT ACGTCGTGGC GAGACGAGCC ACACTGATGT TGCCGTGTAT 
GTGGCTGATA TACTGGAGTC GATGGAGAAA GACGGCATTA CGCAGTTTAC GGCCAATCAG 
CAGGCGGAGA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163551.1
CDS	286..1560
Translation	MAERPKNLFATGSSRSRNPPDQLSLNNLSIRHPPSSTSSTSSGSTSSGSSSSSQHNHVTRCFGAQQPQQTPPVASSQVPPVPAASSSSAADRHRERIRQQACGKLQFGEGGANTHTFTSDDLEDEGEIGRGAFGAVNKMTFKKLDKVMAVKRIRSTVDEKEQKQLLMDLEVVMKSNECIYIVQFYGALFKEGDCWICMELMDTSLDKFYKYIYEKQQRHIPESILAKITVATVNALNYLKEELKIIHRDVKPSNILLHRRGDIKLCDFGISGQLVDSIAKTKDAGCRPYMAPERIDPERAKGYDVRSDVWSLGITLMEVATGNFPYRKWDSVFEQLCQVVQGEPPRLLTSYNGMEFSKEFVDFVNTCLIKKESDRPKYSRLLEMPFIRRGETSHTDVAVYVADILESMEKDGITQFTANQQAES

Target ORF information:

RefSeq Version	NM_001170080.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster MAP kinase kinase 4 (Mkk4), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170080.2

ORF Insert Sequence:

ATGGCCGAAC GACCGAAAAA TTTGTTTGCA ACCGGATCCA GTCGCTCCCG CAATCCCCCG 
GATCAGCTAA GCCTGAATAA TCTTAGCATA CGCCATCCGC CCTCGTCCAC GTCGTCCACT 
TCTTCGGGAT CCACCTCCTC GGGCTCAAGT TCCTCATCAC AGCACAACCA TGTGACCCGC 
TGTTTTGGTG CCCAGCAGCC ACAGCAAACC CCGCCGGTGG CCAGCAGTCA AGTGCCACCG 
GTGCCGGCAG CATCGAGCAG CAGCGCTGCC GATCGTCACC GGGAGCGAAT TCGCCAGCAG 
GCCTGTGGCA AGCTACAGTT TGGTGAGGGC GGCGCTAATA CACACACATT TACTTCAGAC 
GATCTCGAGG ATGAGGGCGA AATTGGACGC GGTGCATTTG GGGCAGTCAA CAAGATGACC 
TTTAAGAAAT TGGACAAGGT GATGGCCGTC AAGCGCATTC GATCCACCGT AGACGAGAAG 
GAACAGAAGC AGCTGCTCAT GGATCTCGAG GTGGTCATGA AGTCCAACGA GTGCATTTAC 
ATTGTTCAGT TCTATGGGGC GCTTTTCAAG GAGGGTGACT GCTGGATTTG TATGGAGCTG 
ATGGACACCT CTTTGGACAA GTTTTACAAG TACATCTACG AAAAGCAGCA GCGCCACATT 
CCAGAGTCTA TACTGGCTAA GATAACAGTG GCCACGGTCA ATGCGCTGAA CTATTTGAAG 
GAGGAGCTAA AGATTATCCA TCGGGATGTG AAACCGAGCA ACATTCTGTT GCACCGTCGC 
GGAGACATAA AGCTGTGCGA TTTTGGTATT TCGGGTCAGC TGGTCGACTC TATTGCCAAG 
ACCAAGGATG CGGGCTGCAG GCCCTACATG GCGCCGGAGC GCATTGATCC AGAACGTGCC 
AAGGGCTACG ATGTACGAAG CGATGTTTGG TCATTGGGCA TCACATTAAT GGAGGTGGCG 
ACTGGTAATT TCCCCTATCG TAAATGGGAC TCTGTTTTCG AGCAGTTGTG CCAGGTTGTG 
CAAGGCGAAC CGCCGCGACT TTTGACATCC TATAACGGCA TGGAGTTTTC CAAAGAGTTT 
GTGGACTTTG TCAATACTTG CCTAATTAAA AAGGAGAGCG ATCGACCCAA GTATAGCCGT 
CTGCTGGAGA TGCCCTTCAT ACGTCGTGGC GAGACGAGCC ACACTGATGT TGCCGTGTAT 
GTGGCTGATA TACTGGAGTC GATGGAGAAA GACGGCATTA CGCAGTTTAC GGCCAATCAG 
CAGGCGGAGA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mkk4 ( NM_058005.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477353.1 Drosophila melanogaster MAP kinase kinase 4 (Mkk4), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa04250
Clone ID Related Accession (Same CDS sequence)	NM_001170080.2 , NM_058005.5 , NM_001275446.1
Accession Version	NM_058005.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	MAP kinase kinase 4, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_058005.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGAAC GACCGAAAAA TTTGTTTGCA ACCGGATCCA GTCGCTCCCG CAATCCCCCG 
GATCAGCTAA GCCTGAATAA TCTTAGCATA CGCCATCCGC CCTCGTCCAC GTCGTCCACT 
TCTTCGGGAT CCACCTCCTC GGGCTCAAGT TCCTCATCAC AGCACAACCA TGTGACCCGC 
TGTTTTGGTG CCCAGCAGCC ACAGCAAACC CCGCCGGTGG CCAGCAGTCA AGTGCCACCG 
GTGCCGGCAG CATCGAGCAG CAGCGCTGCC GATCGTCACC GGGAGCGAAT TCGCCAGCAG 
GCCTGTGGCA AGCTACAGTT TGGTGAGGGC GGCGCTAATA CACACACATT TACTTCAGAC 
GATCTCGAGG ATGAGGGCGA AATTGGACGC GGTGCATTTG GGGCAGTCAA CAAGATGACC 
TTTAAGAAAT TGGACAAGGT GATGGCCGTC AAGCGCATTC GATCCACCGT AGACGAGAAG 
GAACAGAAGC AGCTGCTCAT GGATCTCGAG GTGGTCATGA AGTCCAACGA GTGCATTTAC 
ATTGTTCAGT TCTATGGGGC GCTTTTCAAG GAGGGTGACT GCTGGATTTG TATGGAGCTG 
ATGGACACCT CTTTGGACAA GTTTTACAAG TACATCTACG AAAAGCAGCA GCGCCACATT 
CCAGAGTCTA TACTGGCTAA GATAACAGTG GCCACGGTCA ATGCGCTGAA CTATTTGAAG 
GAGGAGCTAA AGATTATCCA TCGGGATGTG AAACCGAGCA ACATTCTGTT GCACCGTCGC 
GGAGACATAA AGCTGTGCGA TTTTGGTATT TCGGGTCAGC TGGTCGACTC TATTGCCAAG 
ACCAAGGATG CGGGCTGCAG GCCCTACATG GCGCCGGAGC GCATTGATCC AGAACGTGCC 
AAGGGCTACG ATGTACGAAG CGATGTTTGG TCATTGGGCA TCACATTAAT GGAGGTGGCG 
ACTGGTAATT TCCCCTATCG TAAATGGGAC TCTGTTTTCG AGCAGTTGTG CCAGGTTGTG 
CAAGGCGAAC CGCCGCGACT TTTGACATCC TATAACGGCA TGGAGTTTTC CAAAGAGTTT 
GTGGACTTTG TCAATACTTG CCTAATTAAA AAGGAGAGCG ATCGACCCAA GTATAGCCGT 
CTGCTGGAGA TGCCCTTCAT ACGTCGTGGC GAGACGAGCC ACACTGATGT TGCCGTGTAT 
GTGGCTGATA TACTGGAGTC GATGGAGAAA GACGGCATTA CGCAGTTTAC GGCCAATCAG 
CAGGCGGAGA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477353.1
CDS	286..1560
Translation	MAERPKNLFATGSSRSRNPPDQLSLNNLSIRHPPSSTSSTSSGSTSSGSSSSSQHNHVTRCFGAQQPQQTPPVASSQVPPVPAASSSSAADRHRERIRQQACGKLQFGEGGANTHTFTSDDLEDEGEIGRGAFGAVNKMTFKKLDKVMAVKRIRSTVDEKEQKQLLMDLEVVMKSNECIYIVQFYGALFKEGDCWICMELMDTSLDKFYKYIYEKQQRHIPESILAKITVATVNALNYLKEELKIIHRDVKPSNILLHRRGDIKLCDFGISGQLVDSIAKTKDAGCRPYMAPERIDPERAKGYDVRSDVWSLGITLMEVATGNFPYRKWDSVFEQLCQVVQGEPPRLLTSYNGMEFSKEFVDFVNTCLIKKESDRPKYSRLLEMPFIRRGETSHTDVAVYVADILESMEKDGITQFTANQQAES

Target ORF information:

RefSeq Version	NM_058005.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster MAP kinase kinase 4 (Mkk4), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058005.5

ORF Insert Sequence:

ATGGCCGAAC GACCGAAAAA TTTGTTTGCA ACCGGATCCA GTCGCTCCCG CAATCCCCCG 
GATCAGCTAA GCCTGAATAA TCTTAGCATA CGCCATCCGC CCTCGTCCAC GTCGTCCACT 
TCTTCGGGAT CCACCTCCTC GGGCTCAAGT TCCTCATCAC AGCACAACCA TGTGACCCGC 
TGTTTTGGTG CCCAGCAGCC ACAGCAAACC CCGCCGGTGG CCAGCAGTCA AGTGCCACCG 
GTGCCGGCAG CATCGAGCAG CAGCGCTGCC GATCGTCACC GGGAGCGAAT TCGCCAGCAG 
GCCTGTGGCA AGCTACAGTT TGGTGAGGGC GGCGCTAATA CACACACATT TACTTCAGAC 
GATCTCGAGG ATGAGGGCGA AATTGGACGC GGTGCATTTG GGGCAGTCAA CAAGATGACC 
TTTAAGAAAT TGGACAAGGT GATGGCCGTC AAGCGCATTC GATCCACCGT AGACGAGAAG 
GAACAGAAGC AGCTGCTCAT GGATCTCGAG GTGGTCATGA AGTCCAACGA GTGCATTTAC 
ATTGTTCAGT TCTATGGGGC GCTTTTCAAG GAGGGTGACT GCTGGATTTG TATGGAGCTG 
ATGGACACCT CTTTGGACAA GTTTTACAAG TACATCTACG AAAAGCAGCA GCGCCACATT 
CCAGAGTCTA TACTGGCTAA GATAACAGTG GCCACGGTCA ATGCGCTGAA CTATTTGAAG 
GAGGAGCTAA AGATTATCCA TCGGGATGTG AAACCGAGCA ACATTCTGTT GCACCGTCGC 
GGAGACATAA AGCTGTGCGA TTTTGGTATT TCGGGTCAGC TGGTCGACTC TATTGCCAAG 
ACCAAGGATG CGGGCTGCAG GCCCTACATG GCGCCGGAGC GCATTGATCC AGAACGTGCC 
AAGGGCTACG ATGTACGAAG CGATGTTTGG TCATTGGGCA TCACATTAAT GGAGGTGGCG 
ACTGGTAATT TCCCCTATCG TAAATGGGAC TCTGTTTTCG AGCAGTTGTG CCAGGTTGTG 
CAAGGCGAAC CGCCGCGACT TTTGACATCC TATAACGGCA TGGAGTTTTC CAAAGAGTTT 
GTGGACTTTG TCAATACTTG CCTAATTAAA AAGGAGAGCG ATCGACCCAA GTATAGCCGT 
CTGCTGGAGA TGCCCTTCAT ACGTCGTGGC GAGACGAGCC ACACTGATGT TGCCGTGTAT 
GTGGCTGATA TACTGGAGTC GATGGAGAAA GACGGCATTA CGCAGTTTAC GGCCAATCAG 
CAGGCGGAGA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mkk4 ( NM_001275446.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262375.1 Drosophila melanogaster MAP kinase kinase 4 (Mkk4), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa04250
Clone ID Related Accession (Same CDS sequence)	NM_001170080.2 , NM_058005.5 , NM_001275446.1
Accession Version	NM_001275446.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	MAP kinase kinase 4, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGAAC GACCGAAAAA TTTGTTTGCA ACCGGATCCA GTCGCTCCCG CAATCCCCCG 
GATCAGCTAA GCCTGAATAA TCTTAGCATA CGCCATCCGC CCTCGTCCAC GTCGTCCACT 
TCTTCGGGAT CCACCTCCTC GGGCTCAAGT TCCTCATCAC AGCACAACCA TGTGACCCGC 
TGTTTTGGTG CCCAGCAGCC ACAGCAAACC CCGCCGGTGG CCAGCAGTCA AGTGCCACCG 
GTGCCGGCAG CATCGAGCAG CAGCGCTGCC GATCGTCACC GGGAGCGAAT TCGCCAGCAG 
GCCTGTGGCA AGCTACAGTT TGGTGAGGGC GGCGCTAATA CACACACATT TACTTCAGAC 
GATCTCGAGG ATGAGGGCGA AATTGGACGC GGTGCATTTG GGGCAGTCAA CAAGATGACC 
TTTAAGAAAT TGGACAAGGT GATGGCCGTC AAGCGCATTC GATCCACCGT AGACGAGAAG 
GAACAGAAGC AGCTGCTCAT GGATCTCGAG GTGGTCATGA AGTCCAACGA GTGCATTTAC 
ATTGTTCAGT TCTATGGGGC GCTTTTCAAG GAGGGTGACT GCTGGATTTG TATGGAGCTG 
ATGGACACCT CTTTGGACAA GTTTTACAAG TACATCTACG AAAAGCAGCA GCGCCACATT 
CCAGAGTCTA TACTGGCTAA GATAACAGTG GCCACGGTCA ATGCGCTGAA CTATTTGAAG 
GAGGAGCTAA AGATTATCCA TCGGGATGTG AAACCGAGCA ACATTCTGTT GCACCGTCGC 
GGAGACATAA AGCTGTGCGA TTTTGGTATT TCGGGTCAGC TGGTCGACTC TATTGCCAAG 
ACCAAGGATG CGGGCTGCAG GCCCTACATG GCGCCGGAGC GCATTGATCC AGAACGTGCC 
AAGGGCTACG ATGTACGAAG CGATGTTTGG TCATTGGGCA TCACATTAAT GGAGGTGGCG 
ACTGGTAATT TCCCCTATCG TAAATGGGAC TCTGTTTTCG AGCAGTTGTG CCAGGTTGTG 
CAAGGCGAAC CGCCGCGACT TTTGACATCC TATAACGGCA TGGAGTTTTC CAAAGAGTTT 
GTGGACTTTG TCAATACTTG CCTAATTAAA AAGGAGAGCG ATCGACCCAA GTATAGCCGT 
CTGCTGGAGA TGCCCTTCAT ACGTCGTGGC GAGACGAGCC ACACTGATGT TGCCGTGTAT 
GTGGCTGATA TACTGGAGTC GATGGAGAAA GACGGCATTA CGCAGTTTAC GGCCAATCAG 
CAGGCGGAGA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262375.1
CDS	286..1560
Translation	MAERPKNLFATGSSRSRNPPDQLSLNNLSIRHPPSSTSSTSSGSTSSGSSSSSQHNHVTRCFGAQQPQQTPPVASSQVPPVPAASSSSAADRHRERIRQQACGKLQFGEGGANTHTFTSDDLEDEGEIGRGAFGAVNKMTFKKLDKVMAVKRIRSTVDEKEQKQLLMDLEVVMKSNECIYIVQFYGALFKEGDCWICMELMDTSLDKFYKYIYEKQQRHIPESILAKITVATVNALNYLKEELKIIHRDVKPSNILLHRRGDIKLCDFGISGQLVDSIAKTKDAGCRPYMAPERIDPERAKGYDVRSDVWSLGITLMEVATGNFPYRKWDSVFEQLCQVVQGEPPRLLTSYNGMEFSKEFVDFVNTCLIKKESDRPKYSRLLEMPFIRRGETSHTDVAVYVADILESMEKDGITQFTANQQAES

Target ORF information:

RefSeq Version	NM_001275446.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster MAP kinase kinase 4 (Mkk4), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275446.1

ORF Insert Sequence:

ATGGCCGAAC GACCGAAAAA TTTGTTTGCA ACCGGATCCA GTCGCTCCCG CAATCCCCCG 
GATCAGCTAA GCCTGAATAA TCTTAGCATA CGCCATCCGC CCTCGTCCAC GTCGTCCACT 
TCTTCGGGAT CCACCTCCTC GGGCTCAAGT TCCTCATCAC AGCACAACCA TGTGACCCGC 
TGTTTTGGTG CCCAGCAGCC ACAGCAAACC CCGCCGGTGG CCAGCAGTCA AGTGCCACCG 
GTGCCGGCAG CATCGAGCAG CAGCGCTGCC GATCGTCACC GGGAGCGAAT TCGCCAGCAG 
GCCTGTGGCA AGCTACAGTT TGGTGAGGGC GGCGCTAATA CACACACATT TACTTCAGAC 
GATCTCGAGG ATGAGGGCGA AATTGGACGC GGTGCATTTG GGGCAGTCAA CAAGATGACC 
TTTAAGAAAT TGGACAAGGT GATGGCCGTC AAGCGCATTC GATCCACCGT AGACGAGAAG 
GAACAGAAGC AGCTGCTCAT GGATCTCGAG GTGGTCATGA AGTCCAACGA GTGCATTTAC 
ATTGTTCAGT TCTATGGGGC GCTTTTCAAG GAGGGTGACT GCTGGATTTG TATGGAGCTG 
ATGGACACCT CTTTGGACAA GTTTTACAAG TACATCTACG AAAAGCAGCA GCGCCACATT 
CCAGAGTCTA TACTGGCTAA GATAACAGTG GCCACGGTCA ATGCGCTGAA CTATTTGAAG 
GAGGAGCTAA AGATTATCCA TCGGGATGTG AAACCGAGCA ACATTCTGTT GCACCGTCGC 
GGAGACATAA AGCTGTGCGA TTTTGGTATT TCGGGTCAGC TGGTCGACTC TATTGCCAAG 
ACCAAGGATG CGGGCTGCAG GCCCTACATG GCGCCGGAGC GCATTGATCC AGAACGTGCC 
AAGGGCTACG ATGTACGAAG CGATGTTTGG TCATTGGGCA TCACATTAAT GGAGGTGGCG 
ACTGGTAATT TCCCCTATCG TAAATGGGAC TCTGTTTTCG AGCAGTTGTG CCAGGTTGTG 
CAAGGCGAAC CGCCGCGACT TTTGACATCC TATAACGGCA TGGAGTTTTC CAAAGAGTTT 
GTGGACTTTG TCAATACTTG CCTAATTAAA AAGGAGAGCG ATCGACCCAA GTATAGCCGT 
CTGCTGGAGA TGCCCTTCAT ACGTCGTGGC GAGACGAGCC ACACTGATGT TGCCGTGTAT 
GTGGCTGATA TACTGGAGTC GATGGAGAAA GACGGCATTA CGCAGTTTAC GGCCAATCAG 
CAGGCGGAGA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Mkk4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

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ORF Insert Sequence:

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ORF Insert Sequence:

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ORF Insert Sequence:

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