MED31 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED31
Entrez Gene ID	40577
Full Name	Mediator complex subunit 31
Synonyms	BcDNA:GM28912,CG1057,Dmel\CG1057,Med31,Trap18,dSOH1,dTRAP18,med31,p36
General protein information	Preferred Names Mediator complex subunit 31 Names CG1057 gene product from transcript CG1057-RA CG1057-PA CG1057-PB CG1057-PC CG1057-PD CG1057-PE MED31-PA MED31-PB MED31-PC MED31-PD MED31-PE mediator mediator complex subunit 31
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 82C5-82C5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001300230.1	NP_001287159.1	mediator complex subunit 31, isoform E
NM_001275329.1	NP_001262258.1	mediator complex subunit 31, isoform D
NM_141226.4	NP_649483.1	mediator complex subunit 31, isoform A
NM_169013.3	NP_730861.1	mediator complex subunit 31, isoform B
NM_001259979.2	NP_001246908.1	mediator complex subunit 31, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MED31 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED31 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa03843	NM_169013.3 Latest version!	Drosophila melanogaster mediator complex subunit 31, transcript variant B (MED31), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OFa03843	NM_141226.4 Latest version!	Drosophila melanogaster mediator complex subunit 31, transcript variant A (MED31), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OFa03843	NM_001259979.2 Latest version!	Drosophila melanogaster mediator complex subunit 31, transcript variant C (MED31), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OFa08052	NM_001275329.1 Latest version!	Drosophila melanogaster mediator complex subunit 31, transcript variant D (MED31), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OFa22043	NM_001300230.1 Latest version!	Drosophila melanogaster mediator complex subunit 31, transcript variant E (MED31), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED31 ( NM_141226.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649483.1 Drosophila melanogaster mediator complex subunit 31, transcript variant A (MED31), mRNA.

Price & Availability↑

CloneID	OFa03843
Clone ID Related Accession (Same CDS sequence)	NM_141226.4 , NM_169013.3 , NM_001259979.2
Accession Version	NM_141226.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	mediator complex subunit 31, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141226.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAAAA TGTACGGAAA GGGTAAGACT GCTATCGAGT CGGAGGAGCT GCAGAAGCGA 
CGCTGGCAGA TCGAGCTGGA GTTCGTGCAG TGCCTGTCGA ACCCCAACTA TCTAAACTTC 
CTTGCACAGC GTGGATTTTT CAAGGACCAG TCGTTCATCA ACTACCTGAA GTATCTGCAG 
TACTGGAAGG AGCCGGACTA TGCGAAATAC CTCATGTACC CAATGTGCCT CTACTTCCTC 
GATCTGCTGC AGTACGAACA CTTCCGCCGG GAGATCGTCA ACTCGCAGTG CTGCAAGTTC 
ATAGACGACC AGGCCATTCT GCAGTGGCAG CACTACACTC GCAAGCGCAT CAAACTGATC 
GAAAACGTTA CGGCCGCCCA GCAGCAACAA CAGCAGCTTC AGCAGCAACA ACAGCAGGCG 
AACGGCATGG AGGCAGCAAC GGGAGGCGAA TCAGCAGCAC CTACCCCCAA CGTGAACGGG 
TCAGCTTCCA CGGCGGATAG TCAACAGACG TCTTCTGCCC TACAGCCTGT CCAAGCACAG 
CCTGGAAATC CCCAGCAGCA GCAGCAAATC AATGGCGTGG CATCGGGGGC CAACATAAAA 
CTAGAGTTAA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649483.1
CDS	439..1053
Translation	MAKMYGKGKTAIESEELQKRRWQIELEFVQCLSNPNYLNFLAQRGFFKDQSFINYLKYLQYWKEPDYAKYLMYPMCLYFLDLLQYEHFRREIVNSQCCKFIDDQAILQWQHYTRKRIKLIENVTAAQQQQQQLQQQQQQANGMEAATGGESAAPTPNVNGSASTADSQQTSSALQPVQAQPGNPQQQQQINGVASGANIKLELN

Target ORF information:

RefSeq Version	NM_141226.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mediator complex subunit 31, transcript variant A (MED31), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141226.4

ORF Insert Sequence:

ATGGCCAAAA TGTACGGAAA GGGTAAGACT GCTATCGAGT CGGAGGAGCT GCAGAAGCGA 
CGCTGGCAGA TCGAGCTGGA GTTCGTGCAG TGCCTGTCGA ACCCCAACTA TCTAAACTTC 
CTTGCACAGC GTGGATTTTT CAAGGACCAG TCGTTCATCA ACTACCTGAA GTATCTGCAG 
TACTGGAAGG AGCCGGACTA TGCGAAATAC CTCATGTACC CAATGTGCCT CTACTTCCTC 
GATCTGCTGC AGTACGAACA CTTCCGCCGG GAGATCGTCA ACTCGCAGTG CTGCAAGTTC 
ATAGACGACC AGGCCATTCT GCAGTGGCAG CACTACACTC GCAAGCGCAT CAAACTGATC 
GAAAACGTTA CGGCCGCCCA GCAGCAACAA CAGCAGCTTC AGCAGCAACA ACAGCAGGCG 
AACGGCATGG AGGCAGCAAC GGGAGGCGAA TCAGCAGCAC CTACCCCCAA CGTGAACGGG 
TCAGCTTCCA CGGCGGATAG TCAACAGACG TCTTCTGCCC TACAGCCTGT CCAAGCACAG 
CCTGGAAATC CCCAGCAGCA GCAGCAAATC AATGGCGTGG CATCGGGGGC CAACATAAAA 
CTAGAGTTAA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED31 ( NM_169013.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730861.1 Drosophila melanogaster mediator complex subunit 31, transcript variant B (MED31), mRNA.

Price & Availability↑

CloneID	OFa03843
Clone ID Related Accession (Same CDS sequence)	NM_141226.4 , NM_169013.3 , NM_001259979.2
Accession Version	NM_169013.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	mediator complex subunit 31, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169013.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAAAA TGTACGGAAA GGGTAAGACT GCTATCGAGT CGGAGGAGCT GCAGAAGCGA 
CGCTGGCAGA TCGAGCTGGA GTTCGTGCAG TGCCTGTCGA ACCCCAACTA TCTAAACTTC 
CTTGCACAGC GTGGATTTTT CAAGGACCAG TCGTTCATCA ACTACCTGAA GTATCTGCAG 
TACTGGAAGG AGCCGGACTA TGCGAAATAC CTCATGTACC CAATGTGCCT CTACTTCCTC 
GATCTGCTGC AGTACGAACA CTTCCGCCGG GAGATCGTCA ACTCGCAGTG CTGCAAGTTC 
ATAGACGACC AGGCCATTCT GCAGTGGCAG CACTACACTC GCAAGCGCAT CAAACTGATC 
GAAAACGTTA CGGCCGCCCA GCAGCAACAA CAGCAGCTTC AGCAGCAACA ACAGCAGGCG 
AACGGCATGG AGGCAGCAAC GGGAGGCGAA TCAGCAGCAC CTACCCCCAA CGTGAACGGG 
TCAGCTTCCA CGGCGGATAG TCAACAGACG TCTTCTGCCC TACAGCCTGT CCAAGCACAG 
CCTGGAAATC CCCAGCAGCA GCAGCAAATC AATGGCGTGG CATCGGGGGC CAACATAAAA 
CTAGAGTTAA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730861.1
CDS	164..778
Translation	MAKMYGKGKTAIESEELQKRRWQIELEFVQCLSNPNYLNFLAQRGFFKDQSFINYLKYLQYWKEPDYAKYLMYPMCLYFLDLLQYEHFRREIVNSQCCKFIDDQAILQWQHYTRKRIKLIENVTAAQQQQQQLQQQQQQANGMEAATGGESAAPTPNVNGSASTADSQQTSSALQPVQAQPGNPQQQQQINGVASGANIKLELN

Target ORF information:

RefSeq Version	NM_169013.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mediator complex subunit 31, transcript variant B (MED31), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169013.3

ORF Insert Sequence:

ATGGCCAAAA TGTACGGAAA GGGTAAGACT GCTATCGAGT CGGAGGAGCT GCAGAAGCGA 
CGCTGGCAGA TCGAGCTGGA GTTCGTGCAG TGCCTGTCGA ACCCCAACTA TCTAAACTTC 
CTTGCACAGC GTGGATTTTT CAAGGACCAG TCGTTCATCA ACTACCTGAA GTATCTGCAG 
TACTGGAAGG AGCCGGACTA TGCGAAATAC CTCATGTACC CAATGTGCCT CTACTTCCTC 
GATCTGCTGC AGTACGAACA CTTCCGCCGG GAGATCGTCA ACTCGCAGTG CTGCAAGTTC 
ATAGACGACC AGGCCATTCT GCAGTGGCAG CACTACACTC GCAAGCGCAT CAAACTGATC 
GAAAACGTTA CGGCCGCCCA GCAGCAACAA CAGCAGCTTC AGCAGCAACA ACAGCAGGCG 
AACGGCATGG AGGCAGCAAC GGGAGGCGAA TCAGCAGCAC CTACCCCCAA CGTGAACGGG 
TCAGCTTCCA CGGCGGATAG TCAACAGACG TCTTCTGCCC TACAGCCTGT CCAAGCACAG 
CCTGGAAATC CCCAGCAGCA GCAGCAAATC AATGGCGTGG CATCGGGGGC CAACATAAAA 
CTAGAGTTAA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED31 ( NM_001259979.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246908.1 Drosophila melanogaster mediator complex subunit 31, transcript variant C (MED31), mRNA.

Price & Availability↑

CloneID	OFa03843
Clone ID Related Accession (Same CDS sequence)	NM_141226.4 , NM_169013.3 , NM_001259979.2
Accession Version	NM_001259979.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	mediator complex subunit 31, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001259979.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAAAA TGTACGGAAA GGGTAAGACT GCTATCGAGT CGGAGGAGCT GCAGAAGCGA 
CGCTGGCAGA TCGAGCTGGA GTTCGTGCAG TGCCTGTCGA ACCCCAACTA TCTAAACTTC 
CTTGCACAGC GTGGATTTTT CAAGGACCAG TCGTTCATCA ACTACCTGAA GTATCTGCAG 
TACTGGAAGG AGCCGGACTA TGCGAAATAC CTCATGTACC CAATGTGCCT CTACTTCCTC 
GATCTGCTGC AGTACGAACA CTTCCGCCGG GAGATCGTCA ACTCGCAGTG CTGCAAGTTC 
ATAGACGACC AGGCCATTCT GCAGTGGCAG CACTACACTC GCAAGCGCAT CAAACTGATC 
GAAAACGTTA CGGCCGCCCA GCAGCAACAA CAGCAGCTTC AGCAGCAACA ACAGCAGGCG 
AACGGCATGG AGGCAGCAAC GGGAGGCGAA TCAGCAGCAC CTACCCCCAA CGTGAACGGG 
TCAGCTTCCA CGGCGGATAG TCAACAGACG TCTTCTGCCC TACAGCCTGT CCAAGCACAG 
CCTGGAAATC CCCAGCAGCA GCAGCAAATC AATGGCGTGG CATCGGGGGC CAACATAAAA 
CTAGAGTTAA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246908.1
CDS	159..773
Translation	MAKMYGKGKTAIESEELQKRRWQIELEFVQCLSNPNYLNFLAQRGFFKDQSFINYLKYLQYWKEPDYAKYLMYPMCLYFLDLLQYEHFRREIVNSQCCKFIDDQAILQWQHYTRKRIKLIENVTAAQQQQQQLQQQQQQANGMEAATGGESAAPTPNVNGSASTADSQQTSSALQPVQAQPGNPQQQQQINGVASGANIKLELN

Target ORF information:

RefSeq Version	NM_001259979.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mediator complex subunit 31, transcript variant C (MED31), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259979.2

ORF Insert Sequence:

ATGGCCAAAA TGTACGGAAA GGGTAAGACT GCTATCGAGT CGGAGGAGCT GCAGAAGCGA 
CGCTGGCAGA TCGAGCTGGA GTTCGTGCAG TGCCTGTCGA ACCCCAACTA TCTAAACTTC 
CTTGCACAGC GTGGATTTTT CAAGGACCAG TCGTTCATCA ACTACCTGAA GTATCTGCAG 
TACTGGAAGG AGCCGGACTA TGCGAAATAC CTCATGTACC CAATGTGCCT CTACTTCCTC 
GATCTGCTGC AGTACGAACA CTTCCGCCGG GAGATCGTCA ACTCGCAGTG CTGCAAGTTC 
ATAGACGACC AGGCCATTCT GCAGTGGCAG CACTACACTC GCAAGCGCAT CAAACTGATC 
GAAAACGTTA CGGCCGCCCA GCAGCAACAA CAGCAGCTTC AGCAGCAACA ACAGCAGGCG 
AACGGCATGG AGGCAGCAAC GGGAGGCGAA TCAGCAGCAC CTACCCCCAA CGTGAACGGG 
TCAGCTTCCA CGGCGGATAG TCAACAGACG TCTTCTGCCC TACAGCCTGT CCAAGCACAG 
CCTGGAAATC CCCAGCAGCA GCAGCAAATC AATGGCGTGG CATCGGGGGC CAACATAAAA 
CTAGAGTTAA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED31 ( NM_001275329.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262258.1 Drosophila melanogaster mediator complex subunit 31, transcript variant D (MED31), mRNA.

Price & Availability↑

CloneID	OFa08052
Clone ID Related Accession (Same CDS sequence)	NM_001275329.1
Accession Version	NM_001275329.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	mediator complex subunit 31, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAAAA TGTACGGAAA GGCTGCTATC GAGTCGGAGG AGCTGCAGAA GCGACGCTGG 
CAGATCGAGC TGGAGTTCGT GCAGTGCCTG TCGAACCCCA ACTATCTAAA CTTCCTTGCA 
CAGCGTGGAT TTTTCAAGGA CCAGTCGTTC ATCAACTACC TGAAGTATCT GCAGTACTGG 
AAGGAGCCGG ACTATGCGAA ATACCTCATG TACCCAATGT GCCTCTACTT CCTCGATCTG 
CTGCAGTACG AACACTTCCG CCGGGAGATC GTCAACTCGC AGTGCTGCAA GTTCATAGAC 
GACCAGGCCA TTCTGCAGTG GCAGCACTAC ACTCGCAAGC GCATCAAACT GATCGAAAAC 
GTTACGGCCG CCCAGCAGCA ACAACAGCAG CTTCAGCAGC AACAACAGCA GGCGAACGGC 
ATGGAGGCAG CAACGGGAGG CGAATCAGCA GCACCTACCC CCAACGTGAA CGGGTCAGCT 
TCCACGGCGG ATAGTCAACA GACGTCTTCT GCCCTACAGC CTGTCCAAGC ACAGCCTGGA 
AATCCCCAGC AGCAGCAGCA AATCAATGGC GTGGCATCGG GGGCCAACAT AAAACTAGAG 
TTAAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262258.1
CDS	439..1047
Translation	MAKMYGKAAIESEELQKRRWQIELEFVQCLSNPNYLNFLAQRGFFKDQSFINYLKYLQYWKEPDYAKYLMYPMCLYFLDLLQYEHFRREIVNSQCCKFIDDQAILQWQHYTRKRIKLIENVTAAQQQQQQLQQQQQQANGMEAATGGESAAPTPNVNGSASTADSQQTSSALQPVQAQPGNPQQQQQINGVASGANIKLELN

Target ORF information:

RefSeq Version	NM_001275329.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mediator complex subunit 31, transcript variant D (MED31), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275329.1

ORF Insert Sequence:

ATGGCCAAAA TGTACGGAAA GGCTGCTATC GAGTCGGAGG AGCTGCAGAA GCGACGCTGG 
CAGATCGAGC TGGAGTTCGT GCAGTGCCTG TCGAACCCCA ACTATCTAAA CTTCCTTGCA 
CAGCGTGGAT TTTTCAAGGA CCAGTCGTTC ATCAACTACC TGAAGTATCT GCAGTACTGG 
AAGGAGCCGG ACTATGCGAA ATACCTCATG TACCCAATGT GCCTCTACTT CCTCGATCTG 
CTGCAGTACG AACACTTCCG CCGGGAGATC GTCAACTCGC AGTGCTGCAA GTTCATAGAC 
GACCAGGCCA TTCTGCAGTG GCAGCACTAC ACTCGCAAGC GCATCAAACT GATCGAAAAC 
GTTACGGCCG CCCAGCAGCA ACAACAGCAG CTTCAGCAGC AACAACAGCA GGCGAACGGC 
ATGGAGGCAG CAACGGGAGG CGAATCAGCA GCACCTACCC CCAACGTGAA CGGGTCAGCT 
TCCACGGCGG ATAGTCAACA GACGTCTTCT GCCCTACAGC CTGTCCAAGC ACAGCCTGGA 
AATCCCCAGC AGCAGCAGCA AATCAATGGC GTGGCATCGG GGGCCAACAT AAAACTAGAG 
TTAAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED31 ( NM_001300230.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287159.1 Drosophila melanogaster mediator complex subunit 31, transcript variant E (MED31), mRNA.

Price & Availability↑

CloneID	OFa22043
Clone ID Related Accession (Same CDS sequence)	NM_001300230.1
Accession Version	NM_001300230.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	mediator complex subunit 31, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACTCCT ACGCTGCTAT CGAGTCGGAG GAGCTGCAGA AGCGACGCTG GCAGATCGAG 
CTGGAGTTCG TGCAGTGCCT GTCGAACCCC AACTATCTAA ACTTCCTTGC ACAGCGTGGA 
TTTTTCAAGG ACCAGTCGTT CATCAACTAC CTGAAGTATC TGCAGTACTG GAAGGAGCCG 
GACTATGCGA AATACCTCAT GTACCCAATG TGCCTCTACT TCCTCGATCT GCTGCAGTAC 
GAACACTTCC GCCGGGAGAT CGTCAACTCG CAGTGCTGCA AGTTCATAGA CGACCAGGCC 
ATTCTGCAGT GGCAGCACTA CACTCGCAAG CGCATCAAAC TGATCGAAAA CGTTACGGCC 
GCCCAGCAGC AACAACAGCA GCTTCAGCAG CAACAACAGC AGGCGAACGG CATGGAGGCA 
GCAACGGGAG GCGAATCAGC AGCACCTACC CCCAACGTGA ACGGGTCAGC TTCCACGGCG 
GATAGTCAAC AGACGTCTTC TGCCCTACAG CCTGTCCAAG CACAGCCTGG AAATCCCCAG 
CAGCAGCAGC AAATCAATGG CGTGGCATCG GGGGCCAACA TAAAACTAGA GTTAAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287159.1
CDS	34..633
Translation	MNSYAAIESEELQKRRWQIELEFVQCLSNPNYLNFLAQRGFFKDQSFINYLKYLQYWKEPDYAKYLMYPMCLYFLDLLQYEHFRREIVNSQCCKFIDDQAILQWQHYTRKRIKLIENVTAAQQQQQQLQQQQQQANGMEAATGGESAAPTPNVNGSASTADSQQTSSALQPVQAQPGNPQQQQQINGVASGANIKLELN

Target ORF information:

RefSeq Version	NM_001300230.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mediator complex subunit 31, transcript variant E (MED31), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300230.1

ORF Insert Sequence:

ATGAACTCCT ACGCTGCTAT CGAGTCGGAG GAGCTGCAGA AGCGACGCTG GCAGATCGAG 
CTGGAGTTCG TGCAGTGCCT GTCGAACCCC AACTATCTAA ACTTCCTTGC ACAGCGTGGA 
TTTTTCAAGG ACCAGTCGTT CATCAACTAC CTGAAGTATC TGCAGTACTG GAAGGAGCCG 
GACTATGCGA AATACCTCAT GTACCCAATG TGCCTCTACT TCCTCGATCT GCTGCAGTAC 
GAACACTTCC GCCGGGAGAT CGTCAACTCG CAGTGCTGCA AGTTCATAGA CGACCAGGCC 
ATTCTGCAGT GGCAGCACTA CACTCGCAAG CGCATCAAAC TGATCGAAAA CGTTACGGCC 
GCCCAGCAGC AACAACAGCA GCTTCAGCAG CAACAACAGC AGGCGAACGG CATGGAGGCA 
GCAACGGGAG GCGAATCAGC AGCACCTACC CCCAACGTGA ACGGGTCAGC TTCCACGGCG 
GATAGTCAAC AGACGTCTTC TGCCCTACAG CCTGTCCAAG CACAGCCTGG AAATCCCCAG 
CAGCAGCAGC AAATCAATGG CGTGGCATCG GGGGCCAACA TAAAACTAGA GTTAAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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MED31 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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